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swarming motility

Tricia A Van Laar, Saika Esani, Tyler J Birges, Bethany Hazen, Jason M Thomas, Mamta Rawat
Pseudomonas aeruginosa is a ubiquitous Gram-negative bacterium that can cause severe opportunistic infections. The principal redox buffer employed by this organism is glutathione (GSH). To assess the role of GSH in the virulence of P. aeruginosa , a number of analyses were performed using a mutant strain deficient in gshA , which does not produce GSH. The mutant strain exhibited a growth delay in minimal medium compared to the wild-type strain. Furthermore, the gshA mutant was defective in biofilm and persister cell formation and in swimming and swarming motility and produced reduced levels of pyocyanin, a key virulence factor...
April 25, 2018: MSphere
Sina Rütschlin, Sandra Gunesch, Thomas Boettcher
Bacteria compete for ferric iron by producing siderophores and some microbes engage in piracy by scavenging sidero-phores of their competitors. The macrocyclic hydroxamate siderophore avaroferrin of Shewanella algae inhibits swarm-ing of Vibrio alginolyticus by evading this piracy. Avaroferrin as well as related putrebactin and bisucaberin are produced by the IucC-like synthetases AvbD, PubC, and BibCC. Here, we established that they are capable of synthesizing not only their native product but also other siderophores...
April 13, 2018: ACS Chemical Biology
Yiyang Yu, Fang Yan, Yinghao He, Yuxuan Qin, Yun Chen, Yunrong Chai, Jian-Hua Guo
ATP-dependent proteases play essential roles in both protein quality control and the regulation of protein activities in bacteria. ClpYQ (also known as HslVU) is one of several highly conserved ATP-dependent proteases in bacteria. The regulation and biological function of ClpYQ have been well studied in Gram-negative bacteria, but are poorly understood in Gram-positive species. In this study, we showed that in the Gram-positive bacterium Bacillus subtilis, the ΔclpYQ deletion mutant formed early and robust biofilms, while swarming motility was severely impaired...
April 9, 2018: Microbiology
Wioletta Lisicka, Jakub Fikowicz-Krosko, Sylwia Jafra, Magdalena Narajczyk, Paulina Czaplewska, Robert Czajkowski
Dickeya solani is a Gram-negative necrotrophic, plant pathogenic bacterium able to cause symptoms in a variety of plant species worldwide. As a facultative anaerobe, D. solani is able to infect hosts under a broad range of oxygen concentrations found in plant environments. However, little is known about oxygen-dependent gene expression in Dickeya spp. that might contribute to its success as a pathogen. Using a Tn5 transposon, harboring a promoterless gusA reporter gene, 146 mutants of D. solani IPO2222 were identified that exhibited oxygen-regulated expression of the gene into which the insertion had occurred...
2018: Frontiers in Plant Science
Devin B Holman, Shawn M D Bearson, Bradley L Bearson, Brian W Brunelle
Background: Multidrug-resistant (MDR) Salmonella enterica serovar Typhimurium ( S . Typhimurium) is a serious public health threat as infections caused by these strains are more difficult and expensive to treat. Livestock serve as a reservoir for MDR Salmonella , and the antibiotics chlortetracycline and florfenicol are frequently administrated to food-producing animals to treat and prevent various diseases. Therefore, we evaluated the response of MDR S . Typhimurium after exposure to these two antibiotics...
2018: Gut Pathogens
Yongjin Liu, Steven Kyle, Paul D Straight
Competitive interactions between bacteria reveal physiological adaptations that benefit fitness. Bacillus subtilis is a Gram-positive species with several adaptive mechanisms for competition and environmental stress. Biofilm formation, sporulation, and motility are the outcomes of widespread changes in a population of B. subtilis . These changes emerge from complex, regulated pathways for adapting to external stresses, including competition from other species. To identify competition-specific functions, we cultured B...
January 2018: MSphere
Mengmeng Zhang, Mengjia Wang, Xiaocui Zhu, Wengong Yu, Qianhong Gong
OBJECTIVE: To screen for the quorum-sensing (QS) inhibitors from marine-derived fungi and evaluate their anti-QS properties in Pseudomonas aeruginosa. RESULTS: QS inhibitory activity was found in secondary metabolites of a marine fungus Fusarium sp. Z10 using P. aeruginosa QSIS-lasI biosensor. The major active compound of this fungus was isolated by HPLC and identified as equisetin. Subinhibitory concentration of equisetin could inhibit the formation of biofilm, swarming motility, and the production of virulence factors in P...
March 3, 2018: Biotechnology Letters
Li Liu, Tao Li, Xing-Jun Cheng, Cui-Ting Peng, Chang-Cheng Li, Li-Hui He, Si-Min Ju, Ning-Yu Wang, Ting-Hong Ye, Mao Lian, Qing-Jie Xiao, Ying-Jie Song, Yi-Bo Zhu, Luo-Ting Yu, Zhen-Ling Wang, Rui Bao
DspI, a putative enoyl-coenzyme A (CoA) hydratase/isomerase, was proposed to be involved in the synthesis of cis-2-decenoic acid (CDA), a quorum sensing (QS) signal molecule in the pathogen Pseudomonas aeruginosa (P. aeruginosa). The present study provided a structural basis for the dehydration reaction mechanism of DspI during CDA synthesis. Structural analysis reveals that Glu126, Glu146, Cys127, Cys131 and Cys154 are important for its enzymatic function. Moreover, we show that the deletion of dspI results in a remarkable decreased in the pyoverdine production, flagella-dependent swarming motility, and biofilm dispersion as well as attenuated virulence in P...
March 2, 2018: Scientific Reports
Marilena Falcone, Silvia Ferrara, Elio Rossi, Helle K Johansen, Søren Molin, Giovanni Bertoni
The small RNA ErsA of Pseudomonas aeruginosa was previously suggested to be involved in biofilm formation via negative post-transcriptional regulation of the algC gene that encodes the virulence-associated enzyme AlgC, which provides sugar precursors for the synthesis of several polysaccharides. In this study, we show that a knock-out ersA mutant strain forms a flat and uniform biofilm, not characterized by mushroom-multicellular structures typical of a mature biofilm. Conversely, the knock-out mutant strain showed enhanced swarming and twitching motilities...
2018: Frontiers in Microbiology
Roya Vahedi-Shahandashti, Rouha Kasra-Kermanshahi, Maliheh Shokouhfard, Parinaz Ghadam, Mohammad Mehdi Feizabadi, Shahram Teimourian
Background and Objectives: Serratia marcescens , a potentially pathogenic bacterium, benefits from its swarming motility and resistance to antibiotic as two important virulence factors. Inappropriate use of antibiotics often results in drug resistance phenomenon in bacterial population. Use of probiotic bacteria has been recommended as partial replacement. In this study, we investigated the effects of some lactobacilli culture supernatant on swarming, motility and antibiotic resistance of S...
December 2017: Iranian Journal of Microbiology
Wang Guoqi, Li Zhirui, Wang Song, Li Tongtong, Zhang Lihai, Zhang Licheng, Tang Peifu
Pseudomonas aeruginosa motility, virulence factors and biofilms are known to be detrimental to wound healing. The efficacy of negative pressure wound therapy (NPWT) against P. aeruginosa has been little studied, either in vitro or in vivo. The present study evaluated the effect of negative pressure (NP) on P. aeruginosa motility in vitro, and the effect of NPWT on virulence factors and biofilms in vivo. P. aeruginosa motility was quantified under different levels of NP (atmospheric pressure, - 75, - 125, - 200 mmHg) using an in vitro model...
February 21, 2018: Antonie Van Leeuwenhoek
Anne E Mattingly, Nachiket Kamatkar, Bradley R Borlee, Joshua D Shrout
Pseudomonas aeruginosa exhibits flagellar-mediated swimming in liquid and swarming on hydrated surfaces under diverse nutrient conditions. Prior studies have implicated a phosphodiesterase, DipA, in regulating these flagella-mediated motilities, but collectively, the necessity for DipA was unclear. In this study, we find that the media composition conditionally constrains the influence of DipA on flagellar motility. We show that DipA exhibits more influence on minimal media supplemented with glutamate or glucose where flagellar motility was negated for the dipA mutant...
February 2, 2018: Applied and Environmental Microbiology
Chelsie E Armbruster, Harry L T Mobley, Melanie M Pearson
Proteus mirabilis , a Gram-negative rod-shaped bacterium most noted for its swarming motility and urease activity, frequently causes catheter-associated urinary tract infections (CAUTIs) that are often polymicrobial. These infections may be accompanied by urolithiasis, the development of bladder or kidney stones due to alkalinization of urine from urease-catalyzed urea hydrolysis. Adherence of the bacterium to epithelial and catheter surfaces is mediated by 17 different fimbriae, most notably MR/P fimbriae...
February 2018: EcoSal Plus
Tingting Li, Dangfeng Wang, Nan Liu, Yan Ma, Ting Ding, Yongchao Mei, Jianrong Li
Pseudomonas fluorescens, an important food spoiling bacteria, uses quorum sensing to control biofilm formation and motility. To date, only a few compounds targeting the LuxR-based quorum sensing system of P. fluorescens have been identified. In the present study, the quorum sensing inhibitory effect of cinnamaldehyde at sublethal concentrations was investigated in terms of inhibition of the extracellular protease, biofilm formation, and swimming and swarming motility. The total volatile basic nitrogen value was also measured to evaluate the effect of cinnamaldehyde on quality preservation of turbot fillets stored at 4 ± 1 °C for 15 days...
February 1, 2018: International Journal of Food Microbiology
Felix LewisOscar, Chari Nithya, Sulaiman Ali Alharbi, Naiyf S Alharbi, Nooruddin Thajuddin
Biofilm forming pathogenic bacteria showing resistance towards antimicrobial agents has increased the urge to find an alternative treatment strategy. Among the biofilm forming pathogenic bacteria, Pseudomonas aeruginosa is a well-recognised Gram negative biofilm former causing nosocomial infection and other disease among immunocompromised patients. The aim of the current study is to evaluate the antipathogenic potentials of S. platensis against P. aeruginosa. S. platensis methanolic extract (SME) inhibited the biofilm (89%), extracellular polymeric substances (EPS), cell surface hydrophobicity (CSH) (44%), pyocyanin, pyoveridin and swarming motility of P...
January 31, 2018: Microbial Pathogenesis
Jakia Jannat Keya, Ryuhei Suzuki, Arif Md Rashedul Kabir, Daisuke Inoue, Hiroyuki Asanuma, Kazuki Sada, Henry Hess, Akinori Kuzuya, Akira Kakugo
In nature, swarming behavior has evolved repeatedly among motile organisms because it confers a variety of beneficial emergent properties. These include improved information gathering, protection from predators, and resource utilization. Some organisms, e.g., locusts, switch between solitary and swarm behavior in response to external stimuli. Aspects of swarming behavior have been demonstrated for motile supramolecular systems composed of biomolecular motors and cytoskeletal filaments, where cross-linkers induce large scale organization...
January 31, 2018: Nature Communications
Yimin Hu, Quanxin Cai, Shen Tian, Yong Ge, Zhiming Yuan, Xiaomin Hu
DegS and DegU make up a two component system belonging to a class of signal transduction systems that play important roles in a broad range of bacterial responses to the environment. However, little study has been done to explore the physiological functions of DegS-DegU in mosquitocidal Lysinibacillus sphaericus. In this study, it was found that deletion of degU or degS-degU inhibited the swarming motility, biofilm formation, sporulation and binary toxin production through regulating the related genes, and phosphorylation was necessary for the functions of DegU...
January 25, 2018: Research in Microbiology
Cibelle B Dallagassa, Monica Surek, Bruno S Vizzotto, Karoline C Prediger, Bárbara Moriel, Suélen Wolf, Vinícius Weiss, Leonardo M Cruz, Flávia E A Assis, Katia S Paludo, Fabiane G M Rego, Sônia M S S Farah, Geraldo Picheth, Emanuel M Souza, Fábio O Pedrosa, Leda S Chubatsu, Cyntia M T Fadel-Picheth
Aeromonas are ubiquitous in aquatic habitats. However some species can cause infections in humans, but rarely meningitis. Here we describe the isolation and characterization of an Aeromonas strain from cerebrospinal fluid of a meningitis patient. The isolate, identified as A. trota by biochemical and molecular methods, was susceptible to ampicillin but resistant to cephalothin and cefazolin. Genome sequencing revealed virulence factor genes such as type VI secretion system, aerolysin and lateral flagella. The isolate exhibited swarming motility, hemolytic activity and adhesion and cytotoxicity on HeLa cells...
March 2018: Microbial Pathogenesis
Mingfa Lv, Yufan Chen, Lisheng Liao, Zhibin Liang, Zurong Shi, Yingxin Tang, Sixuan Ye, Jianuan Zhou, Lianhui Zhang
Dickeya zeae is the causal agent of rice foot rot disease, which has recently become a great threat to rice planting countries and regions. The pathogen produces a family of phytotoxins named zeamines that is critical for bacterial virulence, but little is known about the signaling pathways and regulatory mechanisms that govern zeamine production. In this study, we showed that a conserved transcriptional regulator Fis is involved in the regulation of zeamine production in D. zeae strain EC1. Deletion mutants were markedly attenuated in the virulence against rice seed germination...
January 10, 2018: Scientific Reports
Anna C Hughes, Sundharraman Subramanian, Charles E Dann, Daniel B Kearns
SwrA is the master activator of flagellar biosynthesis in Bacillus subtilis , and SwrA activity is restricted by regulatory proteolysis in liquid environments. SwrA is proteolyzed by the LonA protease but requires a proteolytic adaptor protein, SmiA. Here, we show that SwrA and SmiA interact directly. To better understand SwrA activity, SwrA was randomly mutagenized and loss-of-function and gain-of-function mutants were localized primarily to the predicted unstructured C-terminal region. The loss-of-function mutations impaired swarming motility and activation from the Pfla-che promoter...
March 15, 2018: Journal of Bacteriology
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