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https://www.readbyqxmd.com/read/28819217/anti-biofilm-effects-of-anthranilate-on-a-broad-range-of-bacteria
#1
Xi-Hui Li, Soo-Kyoung Kim, Joon-Hee Lee
Anthranilate, one of tryptophan degradation products has been reported to interfere with biofilm formation by Pseudomonas aeruginosa. Here, we investigated the effects of anthranilate on biofilm formation by various bacteria and the mechanisms responsible. Anthranilate commonly inhibited biofilm formation by P. aeruginosa, Vibrio vulnificus, Bacillus subtilis, Salmonella enterica serovar Typhimurium, and Staphylococcus aureus, and disrupted biofilms preformed by these bacteria. Because anthranilate reduced intracellular c-di-GMP and enhanced swimming and swarming motilities in P...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28819178/curcumin-liposomes-interfere-with-quorum-sensing-system-of-aeromonas-sobria-and-in-silico-analysis
#2
Ting Ding, Tingting Li, Zhi Wang, Jianrong Li
Aeromonas sobria is opportunistic pathogen frequently found in environment and food. Interfering with its quorum sensing (QS) system could be a promising way to alleviate its virulence. In this study, curcumin liposomes were prepared and their characteristics like particle size, zeta potential, PDI (Polymey Disperse Index), encapsulation efficiency and loading capacity were measured. The quorum sensing inhibitory effect of curcumin liposomes under sub-MIC (Minimum Inhibitory Concentration) on siderophore production, swimming and swarming motility, extracellular proteases, biofilm formation and AHLs (N-acylhomoserine lactones) production of A...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28812258/chromosomal-flhb1-gene-of-the-alphaproteobacterium-azospirillum-brasilense-sp245-is-essential-for-correct-assembly-of-both-constitutive-polar-flagellum-and-inducible-lateral-flagella
#3
Yulia Filip'echeva, Andrei Shelud'ko, Alexei Prilipov, Elizaveta Telesheva, Dmitry Mokeev, Andrei Burov, Lilia Petrova, Elena Katsy
Azospirillum brasilense has the ability of swimming and swarming motility owing to the work of a constitutive polar flagellum and inducible lateral flagella, respectively. The interplay between these flagellar systems is poorly understood. One of the key elements of the flagellar export apparatus is the protein FlhB. Two predicted flhB genes are present in the genome of A. brasilense Sp245 (accession nos. HE577327-HE577333). Experimental evidence obtained here indicates that the chromosomal coding sequence (CDS) AZOBR_150177 (flhB1) of Sp245 is essential for the production of both types of flagella...
August 15, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28807106/developmental-adaptations-of-trypanosome-motility-to-the-tsetse-fly-host-environments-unravel-a-multifaceted-in-vivo-microswimmer-system
#4
Sarah Schuster, Timothy Krüger, Ines Subota, Sina Thusek, Brice Rotureau, Andreas Beilhack, Markus Engstler
The highly motile and versatile protozoan pathogen Trypanosoma brucei undergoes a complex life cycle in the tsetse fly. Here we introduce the host insect as an expedient model environment for microswimmer research, as it allows examination of microbial motion within a diversified, secluded and yet microscopically tractable space. During their week-long journey through the different microenvironments of the fly´s interior organs, the incessantly swimming trypanosomes cross various barriers and confined surroundings, with concurrently occurring major changes of parasite cell architecture...
August 15, 2017: ELife
https://www.readbyqxmd.com/read/28804018/anti-biofilm-activity-of-a22-s-3-4-dichlorobenzyl-isothiourea-hydrochloride-against-pseudomonas-aeruginosa-influence-on-biofilm-formation-motility-and-bioadhesion
#5
Pauline Cordenonsi Bonez, Grazielle Guidolin Rossi, Jardel Rodrigo Bandeira, Andiara Prates Ramos, Caren Rigon Mizdal, Vanessa Albertina Agertt, Eloísa Salete Segatto Dalla Nora, Márcia Ebling de Souza, Camilla Fillippi Dos Santos Alves, Fallon Siqueira Dos Santos, André Gündel, Rodrigo de Almeida Vaucher, Roberto Christ Vianna Santos, Marli Matiko Anraku de Campos
Bacterial biofilms are involved in various medical infections and for this reason it is of great importance understanding adhesion mechanisms of involved microorganisms is essential to develop new strategies of prevention and control. Different approaches have been used for preventing biofilm related infections in health care settings, such as use of surface coatings agents in medical implants. In this context, is necessary to explore new compounds with anti-biofilm activity. Thus, this study evaluated for the first time the action of A22 against biofilms of Pseudomonas aeruginosa PAO1 strain and multi-resistant clinical isolates on biotic and abiotic surfaces...
August 10, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28799693/the-small-rna-real-a-novel-regulatory-element-embedded-in-the-pseudomonas-aeruginosa-quorum-sensing-networks
#6
Sara Carloni, Raffaella Macchi, Sara Sattin, Silvia Ferrara, Giovanni Bertoni
The small RNA ReaL of the opportunistic pathogen Pseudomonas aeruginosa has been characterized. Our results indicate that ReaL contributes to P. aeruginosa virulence. In the Galleria mellonella infection model, reaL gene deletion resulted in decreased virulence, while ReaL overexpression resulted in a hyper-virulent phenotype. We also demonstrate that ReaL is embedded in the P. aeruginosa quorum sensing (QS) with the role of linking las to pqs systems. We show that ReaL is negatively regulated by the las regulator LasR and impacts positively the synthesis of the pqs quinolone signal PQS by a positive post-transcriptional effect on the pqsC gene...
August 11, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28780591/revealing-strategies-of-quorum-sensing-in-azospirillum-brasilense-strains-ab-v5-and-ab-v6
#7
Josiane Fukami, Julia Laura Fernandes Abrantes, Pablo Del Cerro, Marco Antonio Nogueira, Francisco Javier Ollero, Manuel Megías, Mariangela Hungria
Azospirillum brasilense is an important plant-growth promoting bacterium (PGPB) that requires several critical steps for root colonization, including biofilm and exopolysaccharide (EPS) synthesis and cell motility. In several bacteria these mechanisms are mediated by quorum sensing (QS) systems that regulate the expression of specific genes mediated by the autoinducers N-acyl-homoserine lactones (AHLs). We investigated QS mechanisms in strains Ab-V5 and Ab-V6 of A. brasilense, which are broadly used in commercial inoculants in Brazil...
August 5, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28769915/the-transient-multidrug-resistance-phenotype-of-salmonella-enterica-swarming-cells-is-abolished-by-sub-inhibitory-concentrations-of-antimicrobial-compounds
#8
Oihane Irazoki, Susana Campoy, Jordi Barbé
Swarming motility is the rapid and coordinated multicellular migration of bacteria across a moist surface. During swarming, bacterial cells exhibit increased resistance to multiple antibiotics, a phenomenon described as adaptive or transient resistance. In this study, we demonstrate that sub-inhibitory concentrations of cefotaxime, ciprofloxacin, trimethoprim, or chloramphenicol, but not that of amikacin, colistin, kanamycin or tetracycline, impair Salmonella enterica swarming. Chloramphenicol-treated S. enterica cells exhibited a clear decrease in their flagellar content, while treatment with other antibiotics that reduced swarming (cefotaxime, ciprofloxacin, and trimethoprim) inhibited polar chemoreceptor array assembly...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28769898/bypassing-the-need-for-the-transcriptional-activator-eara-through-a-spontaneous-deletion-in-the-bre-portion-of-the-fla-operon-promoter-in-methanococcus-maripaludis
#9
Yan Ding, Alison Berezuk, Cezar M Khursigara, Ken F Jarrell
In Methanococcus maripaludis, the euryarchaeal archaellum regulator A (EarA) is required for the transcription of the fla operon, which is comprised of a series of genes which encode most of the proteins needed for the formation of the archaeal swimming organelle, the archaellum. In mutants deleted for earA (ΔearA), there is almost undetectable transcription of the fla operon, Fla proteins are not synthesized and the cells are non-archaellated. In this study, we have isolated a spontaneous mutant of a ΔearA mutant in which the restoration of the transcription and translation of the fla operon (using flaB2, the second gene of the operon, as a reporter), archaella formation and swarming motility were all restored even in the absence of EarA...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28767643/bow-tie-signaling-in-c-di-gmp-machine-learning-in-a-simple-biochemical-network
#10
Jinyuan Yan, Maxime Deforet, Kerry E Boyle, Rayees Rahman, Raymond Liang, Chinweike Okegbe, Lars E P Dietrich, Weigang Qiu, Joao B Xavier
Bacteria of many species rely on a simple molecule, the intracellular secondary messenger c-di-GMP (Bis-(3'-5')-cyclic dimeric guanosine monophosphate), to make a vital choice: whether to stay in one place and form a biofilm, or to leave it in search of better conditions. The c-di-GMP network has a bow-tie shaped architecture that integrates many signals from the outside world-the input stimuli-into intracellular c-di-GMP levels that then regulate genes for biofilm formation or for swarming motility-the output phenotypes...
August 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28758636/the-pseudomonas-aeruginosa-dnak-gene-is-involved-in-bacterial-translocation-across-the-intestinal-epithelial-cell-barrier
#11
Jun Okuda, Satoshi Yamane, Syouya Nagata, Chinami Kunikata, Chigusa Suezawa, Masashi Yasuda
Pseudomonas aeruginosa can penetrate through polarized epithelial cell monolayers produced by the human adenocarcinoma cell line Caco-2. We previously identified genes associated with bacterial translocation through Caco-2 cell monolayers by analysing transposon insertion mutants with dramatically reduced penetration activity relative to that of the wild-type P. aeruginosa PAO1 strain. In this study, we focused on the dnaK mutant because the association between this gene and penetration activity is unknown...
July 31, 2017: Microbiology
https://www.readbyqxmd.com/read/28732084/increased-parb-level-affects-expression-of-stress-response-adaptation-and-virulence-operons-and-potentiates-repression-of-promoters-adjacent-to-the-high-affinity-binding-sites-pars3-and-pars4-in-pseudomonas-aeruginosa
#12
Adam Kawalek, Krzysztof Glabski, Aneta Agnieszka Bartosik, Anna Fogtman, Grazyna Jagura-Burdzy
Similarly to its homologs in other bacteria, Pseudomonas aeruginosa partitioning protein ParB facilitates segregation of newly replicated chromosomes. Lack of ParB is not lethal but results in increased frequency of anucleate cells production, longer division time, cell elongation, altered colony morphology and defective swarming and swimming motility. Unlike in other bacteria, inactivation of parB leads to major changes of the transcriptome, suggesting that, directly or indirectly, ParB plays a role in regulation of gene expression in this organism...
2017: PloS One
https://www.readbyqxmd.com/read/28699375/novel-insights-into-tat-pathway-in-xanthomonas-oryzae-pv-oryzae-stress-adaption-and-virulence-identification-and-characterization-of-tat-dependent-translocation-proteins
#13
Guichun Wu, Panpan Su, Bo Wang, Yuqiang Zhang, Guoliang Qian, Fengquan Liu
Xanthomonas oryzae pv. oryzae, an economically important bacterium, causes a serious disease in rice production worldwide called bacterial leaf blight. How X. oryzae pv. oryzae infects rice and causes symptoms remains incompletely understood. Our earlier works demonstrated that the twin-arginine translocation (Tat) pathway plays an vital role in X. oryzae pv. oryzae fitness and virulence but the underlying mechanism is unknown. In this study, we used strain PXO99(A) as a working model, and identified 15 potential Tat-dependent translocation proteins (TDTP) by using comparative proteomics and bioinformatics analyses...
September 2017: Phytopathology
https://www.readbyqxmd.com/read/28694794/antivirulent-properties-of-underexplored-cinnamomum-tamala-essential-oil-and-its-synergistic-effects-with-dnase-against-pseudomonas-aeruginosa-biofilms-an-in-vitro-study
#14
Sanaulla Farisa Banu, Durairajan Rubini, Sairam Rakshitaa, Kamaraj Chandrasekar, Ramar Murugan, Aruni Wilson, Shanmugaraj Gowrishankar, Shunmugiah Karutha Pandian, Paramasivam Nithyanand
Pseudomonas aeruginosa is a nosocomial pathogen colonizing patients with chronic infectious diseases and has gained resistance to all the known broad spectrum antibiotics available today. The present study showcases the antibiofilm potential of an essential oil (EO) from an underexplored Cinnamomum species namely, C. tamala, against P. aeruginosa biofilms. Furthermore, the synergistic effects of the EO along with a commercially available DNase (DNaseI) and a DNase (MBD) isolated from a marine bacterium were explored for its antibiofilm activity...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28685594/disruption-of-the-quorum-sensing-regulated-pathogenic-traits-of-the-biofilm-forming-fish-pathogen-aeromonas-hydrophila-by-tannic-acid-a-potent-quorum-quencher
#15
Bhakti Patel, Supriya Kumari, Rajanya Banerjee, Mrinal Samanta, Surajit Das
The quorum sensing (QS) phenomenon regulates a myriad of pathogenic traits in the biofilm forming fish pathogen, Aeromonas hydrophila. Blocking the QS mechanism of A. hydrophila is a novel strategy to prevent disease in fish. This study evaluated the effect of tannic acid, a QS inhibitor, on A. hydrophila-associated QS regulated phenomena. A streaking assay with Chromobacterium violaceum (CVO26) reported the presence of N-acyl homoserine lactone (AHL) in A. hydrophila, which was confirmed by HPLC and GC-MS analysis...
July 7, 2017: Biofouling
https://www.readbyqxmd.com/read/28652301/stand-alone-eal-domain-proteins-form-a-distinct-subclass-of-eal-proteins-involved-in-regulation-of-cell-motility-and-biofilm-formation-in-enterobacteria
#16
Youssef El Mouali, Hyunhee Kim, Irfan Ahmad, Annelie Brauner, Ying Liu, Mikael Skurnik, Michael Y Galperin, Ute Römling
The second messenger cyclic di-GMP is almost ubiquitous among bacteria and so are the cyclic di-GMP turnover proteins, which mediate the transition between motility and sessility. EAL domain proteins have been characterized as cyclic di-GMP-specific phosphodiesterases. While most EAL domain proteins contain additional, usually N-terminal, domains, there is a distinct family of proteins within stand-alone EAL domains, exemplified by Salmonella enterica serovar Typhimurium proteins STM3611 (YhjH/PdeH), a c-di-GMP-specific phosphodiesterase, and the enzymatically inactive STM1344 (YdiV/CdgR) and STM1697, which regulate bacterial motility through interaction with the flagella master regulator FlhDC...
June 26, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28637123/inhibition-of-swarming-motility-of-pseudomonas-aeruginosa-by-methanol-extracts-of-alpinia-officinarum-hance-and-cinnamomum-tamala-t-nees-and-eberm
#17
Divya Lakshmanan, Jishudas Nanda, K Jeevaratnam
Bacterial drug resistance is a challenge in clinical settings, especially in countries like India. Hence, discovery of novel alternative therapeutics has become a necessity in the fight against drug resistance. Compounds that inhibit bacterial virulence properties form new therapeutic alternatives. Pseudomonas aeruginosa is an opportunistic, nosocomial pathogen that infects immune-compromised patients. Swarming motility is an important virulence property of Pseudomonas which aids it in reaching host cells under nutrient limiting conditions...
June 15, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28613253/terpenoids-from-platostoma-rotundifolium-briq-a-j-paton-alter-the-expression-of-quorum-sensing-related-virulence-factors-and-the-formation-of-biofilm-in-pseudomonas-aeruginosa-pao1
#18
Tsiry Rasamiravaka, Jérémie Ngezahayo, Laurent Pottier, Sofia Oliveira Ribeiro, Florence Souard, Léonard Hari, Caroline Stévigny, Mondher El Jaziri, Pierre Duez
Platostoma rotundifolium (Briq.) A. J. Paton aerial parts are widely used in Burundi traditional medicine to treat infectious diseases. In order to investigate their probable antibacterial activities, crude extracts from P. rotundifolium were assessed for their bactericidal and anti-virulence properties against an opportunistic bacterial model, Pseudomonas aeruginosa PAO1. Whereas none of the tested extracts exert bacteriostatic and/or bactericidal proprieties, the ethyl acetate and dichloromethane extracts exhibit anti-virulence properties against Pseudomonas aeruginosa PAO1 characterized by an alteration in quorum sensing gene expression and biofilm formation without affecting bacterial viability...
June 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28607157/anaerobic-cysteine-degradation-and-potential-metabolic-coordination-in-salmonella-enterica-and-escherichia-coli
#19
Melissa Loddeke, Barbara Schneider, Tamiko Oguri, Iti Mehta, Zhenyu Xuan, Larry Reitzer
Salmonella enterica has two CyuR-activated enzymes that degrade cysteine, i.e., the aerobic CdsH and an unidentified anaerobic enzyme; Escherichia coli has only the latter. To identify the anaerobic enzyme, transcript profiling was performed for E. coli without cyuR and with overexpressed cyuR Thirty-seven genes showed at least 5-fold changes in expression, and the cyuPA (formerly yhaOM) operon showed the greatest difference. Homology suggested that CyuP and CyuA represent a cysteine transporter and an iron-sulfur-containing cysteine desulfidase, respectively...
August 15, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28593349/transcriptomic-analysis-of-the-swarm-motility-phenotype-of-salmonella-enterica-serovar-typhimurium-mutant-defective-in-periplasmic-glucan-synthesis
#20
Arvind A Bhagwat, Lynn Young, Allen D Smith, Medha Bhagwat
Movement of food-borne pathogens on moist surfaces enables them to migrate towards more favorable niches and facilitate their survival for extended periods of time. Salmonella enterica serovar Typhimurium mutants defective in Osmoregulated periplasmic glucans (OPG) synthesis are unable to exhibit motility on moist surfaces (swarming); however, their mobility in liquid (swim motility) remains unaffected. In order to understand the role of OPG in swarm motility, transcriptomic analysis was performed using cells growing on a moist agar surface...
September 2017: Current Microbiology
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