keyword
MENU ▼
Read by QxMD icon Read
search

swarming motility

keyword
https://www.readbyqxmd.com/read/28318117/transcriptional-regulation-of-cpsq-mfpabc-and-mfpabc-by-calr%C3%A2-in-vibrio-parahaemolyticus
#1
He Gao, Lingyu Zhang, George Osei-Adjei, Wenhui Yang, Dongsheng Zhou, Xinxiang Huang, Huiying Yang, Zhe Yin, Yiquan Zhang
The cpsQ-mfpABC locus is transcribed as two operons, i.e., cpsQ-mfpABC and mfpABC, in Vibrio parahaemolyticus, and both of them are all required for biofilm formation. CalR belongs to the LysR-type transcriptional regulator family, and was originally identified as a repressor of the swarming motility and T3SS1 genes expression in V. parahaemolyticus. In the present work, a combination of qRT-PCR, primer extension, LacZ fusion expression, electrophoretic mobility shift assay, and DNase I footprinting assays were employed to elucidate the regulatory mechanisms of cpsQ-mfpABC and mfpABC by CalR...
March 20, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28294685/star-anise-illicium-verum-hook-f-as-quorum-sensing-and-biofilm-formation-inhibitor-on-foodborne-bacteria-study-in-milk
#2
Md Ramim Tanver Rahman, Zaixiang Lou, Jun Zhang, Fuhao Yu, Yakindra Prasad Timilsena, Caili Zhang, Yi Zhang, Amr M Bakry
Bacteria use quorum sensing (QS) systems to communicate with each other and regulate microbial group behavior, such as the secretion of virulence factors, including biofilm formation. In order to explore safe, edible agents, the potential of star anise (SA) as an anti-QS and antibiofilm agent and its possible application in milk safety were investigated. Staphylococcus aureus , Salmonella Typhimurium, Pseudomonas aeruginosa , and biosensor strain Chromobacterium violaceum were selected as test strains for QS, biofilm, and exopolysaccharide assays...
March 15, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28270264/suppressor-analysis-of-eepr-mutant-defects-reveals-coordinate-regulation-of-secondary-metabolites-and-serralysin-biosynthesis-by-eepr-and-hexs
#3
Robert M Q Shanks, Nicholas A Stella, Roni M Lahr, Marissa A Aston, Kimberly M Brothers, Jake D Callaghan, Cihad Sigindere, Xinyu Liu
The EepR transcription factor positively regulates secondary metabolites and tissue-damaging metalloproteases. To gain insight into mechanisms by which EepR regulates pigment and co-regulated factors, genetic suppressor analysis was performed. Suppressor mutations that restored pigment to the non-pigmented ∆eepR mutant mapped to the hexS ORF. Mutation of hexS also restored haemolysis, swarming motility and protease production to the eepR mutant. HexS is a known direct and negative regulator of secondary metabolites in Serratia marcescens and is a LysR family regulator and an orthologue of LrhA...
February 2017: Microbiology
https://www.readbyqxmd.com/read/28268179/vibrio-parahaemolyticus-calr-down-regulates-the-thermostable-direct-hemolysin-tdh-gene-transcription-and-thereby-inhibits-hemolytic-activity
#4
Yiquan Zhang, Ying Zhang, He Gao, Lingyu Zhang, Zhe Yin, Xinxiang Huang, Dongsheng Zhou, Huiying Yang, Wenhui Yang, Li Wang
TDH, encoded by tdh gene, is a major virulent determinant of V. parahaemolyticus that controls various biological activities, such as hemolytic activity, cytotoxicity, and enterotoxicity. The hemolytic activity on Wagatsuma agar ascribed to TDH is called Kanagawa phenomenon (KP). All KP positive strains contain tdh1 and tdh2 genes, but tdh2 is predominantly responsible for KP. CalR is a regulatory protein that was originally identified as a repressor of swarming motility and T3SS1 gene expression in V. parahaemolyticus...
March 4, 2017: Gene
https://www.readbyqxmd.com/read/28243227/rifampicin-resistance-mutations-in-the-rpob-gene-in-bacillus-velezensis-cc09-have-pleiotropic-effects
#5
Xun-Chao Cai, Huan Xi, Li Liang, Jia-Dong Liu, Chang-Hong Liu, Ya-Rong Xue, Xiang-Yang Yu
Rifampicin resistance (Rif(r)) mutations in the RNA polymerase β subunit (rpoB) gene exhibit pleiotropic phenotypes as a result of their effects on the transcription machinery in prokaryotes. However, the differences in the effects of the mutations on the physiology and metabolism of the bacteria remain unknown. In this study, we isolated seven Rif(r) mutations in rpoB, including six single point mutations (H485Y, H485C, H485D, H485R, Q472R, and S490L) and one double point mutation (S490L/S617F) from vegetative cells of an endophytic strain, Bacillus velezensis CC09...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28236509/synthetic-analogs-of-rhamnolipids-modulate-structured-biofilms-formed-by-rhamnolipid-nonproducing-mutant-of-pseudomonas-aeruginosa
#6
Hewen Zheng, Nischal Singh, Gauri S Shetye, Yucheng Jin, Diana Li, Yan-Yeung Luk
Rhamnolipids secreted by Pseudomonas aeruginosa are required for the bacteria to form biofilm efficiently and form biofilm with internal structures including pores and channels. In this work, we explore the effect of a class of synthetic analogs of rhamnolipids at controlling (promoting and inhibiting) the biofilm formation activities of a non-rhamnolipid-producing strain - rhlA - of P. aeruginosa. This class of rhamnolipid analogs is known to modulate the swarming motilities of wild-type PAO1 and rhlA mutant, but its effect on biofilm formation of rhlA mutant is unknown...
February 1, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28204863/influence-of-twitching-and-swarming-motilities-on-biofilm-formation-in-pseudomonas-strains
#7
Letícia Muner Otton, Marina da Silva Campos, Karine Lena Meneghetti, Gertrudes Corção
The genus Pseudomonas mainly includes opportunistic pathogens that rely on type IV pili as an important virulence factor, which is associated with adherence and biofilm formation. Pseudomonas infections are well known to be persistent and resilient in nature largely because of the tendency of the species to form biofilms. This study aimed at analyzing environmental strains of Pseudomonas genus with respect to their ability to execute twitching and swarming motilities as well as with respect to their ability to form biofilms both in the presence as well as in the absence of furanone, a substance that has the potential to prevent the formation of biofilms...
February 15, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28194138/phenotypic-microdiversity-and-phylogenetic-signal-analysis-of-traits-related-to-social-interaction-in-bacillus-spp-from-sediment-communities
#8
Dolores María Rodríguez-Torres, África Islas-Robles, Zulema Gómez-Lunar, Luis Delaye, Ismael Hernández-González, Valeria Souza, Michael Travisano, Gabriela Olmedo-Álvarez
Understanding the relationship between phylogeny and predicted traits is important to uncover the dimension of the predictive power of a microbial composition approach. Numerous works have addressed the taxonomic composition of bacteria in communities, but little is known about trait heterogeneity in closely related bacteria that co-occur in communities. We evaluated a sample of 467 isolates from the Churince water system of the Cuatro Cienegas Basin (CCB), enriched for Bacillus spp. The 16S rRNA gene revealed a random distribution of taxonomic groups within this genus among 11 sampling sites...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28188589/synergistic-activity-of-sub-inhibitory-concentrations-of-curcumin-with-ceftazidime-and-ciprofloxacin-against-pseudomonas-aeruginosa-quorum-sensing-related-genes-and-virulence-traits
#9
Shekoufeh Roudashti, Habib Zeighami, Hesam Mirshahabi, Shahin Bahari, Ali Soltani, Fakhri Haghi
Quorum sensing (QS) system in Pseudomonas aeruginosa may be an important target for pharmacological intervention. The present study aimed to investigate the synergetic activity of sub-MIC concentrations of curcumin (C) with ceftazidime (CAZ) and ciprofloxacin (CIP) against P. aeroginusa QS system. We determined the MIC and synergistic activity of C, CAZ and CIP against P. aeroginusa PAO1 using broth microdilution and checkerboard titration methods. The activity of sub-MIC (1/4 and 1/16 MIC) concentrations of C on the QS signal molecules was assessed using a reporter strain assay...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28174308/bacillus-subtilis-swarmer-cells-lead-the-swarm-multiply-and-generate-a-trail-of-quiescent-descendants
#10
Lina Hamouche, Soumaya Laalami, Adrian Daerr, Solène Song, I Barry Holland, Simone J Séror, Kassem Hamze, Harald Putzer
Bacteria adopt social behavior to expand into new territory, led by specialized swarmers, before forming a biofilm. Such mass migration of Bacillus subtilis on a synthetic medium produces hyperbranching dendrites that transiently (equivalent to 4 to 5 generations of growth) maintain a cellular monolayer over long distances, greatly facilitating single-cell gene expression analysis. Paradoxically, while cells in the dendrites (nonswarmers) might be expected to grow exponentially, the rate of swarm expansion is constant, suggesting that some cells are not multiplying...
February 7, 2017: MBio
https://www.readbyqxmd.com/read/28167523/the-role-of-cyclic-di-gmp-and-exopolysaccharide-in-type-iv-pilus-dynamics
#11
Jan Ribbe, Amy E Baker, Sebstian Euler, George A O'Toole, Berenike Maier
For Pseudomonas aeruginosa the levels of cyclic di-GMP govern the transition from the planktonic state to biofilm formation. Type IV pili (T4P) are crucial determinants of biofilm structure and dynamics, but it is unknown how the levels of c-di-GMP affect pilus dynamics. Here, we scrutinized how c-di-GMP affects molecular motor properties and adhesive behavior of T4P. By means of retraction, T4P generated forces of ∼ 30 pN. Deletion mutants in the proteins with known roles in biofilm formation, swarming motility and exopolysaccharide (EPS) production, specifically, the diguanulate cyclases sadC and roeA or the c-di-GMP phosphodiesterase bifA, showed only modest effects on velocity or force of T4P retraction...
February 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28166091/oral-polyphosphate-suppresses-bacterial-collagenase-production-and-prevents-anastomotic-leak-due-to-serratia-marcescens-and-pseudomonas-aeruginosa
#12
Sanjiv K Hyoju, Robin E Klabbers, Melissa Aaron, Monika A Krezalek, Alexander Zaborin, Mara Wiegerinck, Neil H Hyman, Olga Zaborina, Harry Van Goor, John C Alverdy
OBJECTIVE: The objective of this study was to determine the effect of polyphosphate on intestinal bacterial collagenase production and anastomotic leak in mice undergoing colon surgery. BACKGROUND: We have previously shown that anastomotic leak can be caused by intestinal pathogens that produce collagenase. Because bacteria harbor sensory systems to detect the extracellular concentration of phosphate which controls their virulence, we tested whether local phosphate administration in the form of polyphosphate could attenuate pathogen virulence and prevent leak without affecting bacterial growth...
February 3, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/28150465/impact-of-ph-regulation-on-multi-copper-oxidase-production-and-swarming-motility-in-the-bacterium-proteus-hauseri-zmd44
#13
Chang Su, Zhiyang Chen, I-Son Ng
Proteus hauseri ZMd44, a biodecolorizing bacterium, has been known to produce electricity and multi-copper oxidase (Mco-laccase) under copper induction. However, optimization and regulation of production have not been explored. This study is the first attempt to evaluate several parameters on biomass and Mco-laccase production of P. hauseri ZMd44. Through orthogonal experiments with Taguchi's L9 found that P. hauseri ZMd44 was sensitive to pH value. The cells grew relatively quickly at pH 7, thus the biomass and Mco-laccase-production reached 1...
February 1, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28149169/anti-quorum-sensing-and-anti-biofilm-activity-of-amomum-tsaoko-amommum-tsao-ko-crevost-et-lemarie-on-foodborne-pathogens
#14
Md Ramim Tanver Rahman, Zaixiang Lou, Fuhao Yu, Peng Wang, Hongxin Wang
Cell-to-cell communication or quorum sensing (QS) leads to biofilm formation and causing other virulence factors which are extreme problems for food safety, biofilm related infectious diseases etc. This study evaluated the anti-QS activity of the Amomum tsaoko extract (0.5-4 mg/ml) by using Chromobacterium violaceum a biosensor strain and biofilm formation by crystal violate assay. Experimental results demonstrated that the overall yield of Amomum tsao-ko extract was 11.33 ± 0.3% (w/w). MIC for Staphylococcus aureus (Gram positive), Salmonella Typhimurium and Pseudomonas aeruginosa (Gram negative) was 1, 2 and 2 mg/ml, respectively...
February 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28138423/achromobacter-xylosoxidans-infection-in-cystic-fibrosis-siblings-with-different-outcomes-case-reports
#15
Mônica Cássia Firmida, Elizabeth Andrade Marques, Robson Souza Leão, Rosana Helena Vicente Pereira, Elenice Rosa Aguiar Rodrigues, Rodolpho Mattos Albano, Tania Wrobel Folescu, Vagner Bernardo, Pedro Daltro, Domenico Capone, Agnaldo José Lopes
INTRODUCTION: The clinical relevance of Achromobacter xylosoxidans infection in cystic fibrosis (CF) remains controversial. This emerging agent in CF has been associated with increased lung inflammation, more frequent exacerbations and more severe lung disease. We describe a pair of CF siblings chronically colonized by the same multilocus genotype of A. xylosoxidans with different clinical courses, and assess whether this species may have developed any virulence traits and antimicrobial resistance that could have contributed to their singular outcomes...
2017: Respiratory Medicine Case Reports
https://www.readbyqxmd.com/read/28127379/antibiofilm-and-antioxidant-activity-of-propolis-and-bud-poplar-resins-versus-pseudomonas-aeruginosa
#16
Stefania De Marco, Miranda Piccioni, Rita Pagiotti, Donatella Pietrella
Pseudomonas aeruginosa is a common biofilm-forming bacterial pathogen implicated in lung, skin, and systemic infections. Biofilms are majorly associated with chronic lung infection, which is the most severe complication in cystic fibrosis patients characterized by drug-resistant biofilms in the bronchial mucus with zones, where reactive oxygen species concentration is increased mainly due to neutrophil activity. Aim of this work is to verify the anti-Pseudomonas property of propolis or bud poplar resins extracts...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28117457/the-aliphatic-amidase-amie-is-involved-in-regulation-of-pseudomonas-aeruginosa-virulence
#17
Thomas Clamens, Thibaut Rosay, Alexandre Crépin, Teddy Grandjean, Takfarinas Kentache, Julie Hardouin, Perrine Bortolotti, Anke Neidig, Marlies Mooij, Mélanie Hillion, Julien Vieillard, Pascal Cosette, Joerg Overhage, Fergal O'Gara, Emeline Bouffartigues, Alain Dufour, Sylvie Chevalier, Benoit Guery, Pierre Cornelis, Marc G J Feuilloley, Olivier Lesouhaitier
We have previously shown that the eukaryotic C-type natriuretic peptide hormone (CNP) regulates Pseudomonas aeruginosa virulence and biofilm formation after binding on the AmiC sensor, triggering the amiE transcription. Herein, the involvement of the aliphatic amidase AmiE in P. aeruginosa virulence regulation has been investigated. The proteome analysis of an AmiE over-producing strain (AmiE(+)) revealed an expression change for 138 proteins, including some that are involved in motility, synthesis of quorum sensing compounds and virulence regulation...
January 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28117390/growth-rate-control-of-flagellar-assembly-in-escherichia-coli-strain-rp437
#18
Martin Sim, Santosh Koirala, David Picton, Henrik Strahl, Paul A Hoskisson, Christopher V Rao, Colin S Gillespie, Phillip D Aldridge
The flagellum is a rotary motor that enables bacteria to swim in liquids and swarm over surfaces. Numerous global regulators control flagellar assembly in response to cellular and environmental factors. Previous studies have also shown that flagellar assembly is affected by the growth-rate of the cell. However, a systematic study has not yet been described under controlled growth conditions. Here, we investigated the effect of growth rate on flagellar assembly in Escherichia coli using steady-state chemostat cultures where we could precisely control the cell growth-rate...
January 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28114301/weak-synchronization-and-large-scale-collective-oscillation-in-dense-bacterial-suspensions
#19
Chong Chen, Song Liu, Xia-Qing Shi, Hugues Chaté, Yilin Wu
Collective oscillatory behaviour is ubiquitous in nature, having a vital role in many biological processes from embryogenesis and organ development to pace-making in neuron networks. Elucidating the mechanisms that give rise to synchronization is essential to the understanding of biological self-organization. Collective oscillations in biological multicellular systems often arise from long-range coupling mediated by diffusive chemicals, by electrochemical mechanisms, or by biomechanical interaction between cells and their physical environment...
February 9, 2017: Nature
https://www.readbyqxmd.com/read/28100305/environment-directed-activation-the-escherichia-coli-flhdc-operon-by-transposons
#20
Zhongge Zhang, Chika Kukita, Mir Zafri Humayun, Milton H Saier
The flagellar system in E. coli K12 is expressed under the control of the flhDC-encoded master regulator, FlhDC. Transposition of insertion sequence (IS) elements to the upstream flhDC promoter region up-regulates transcription of this operon, resulting in more rapid motility. Wang and Wood (2011) provided evidence that insertion of IS5 into upstream activating sites occurs at higher rates in semisolid agar media in which swarming behavior is allowed as compared with liquid or solid media where swarming cannot occur...
January 17, 2017: Microbiology
keyword
keyword
117686
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"