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https://www.readbyqxmd.com/read/29203495/pa5470-counteracts-antimicrobial-effect-of-azithromycin-by-releasing-stalled-ribosome-in-pseudomonas-aeruginosa
#1
Jing Shi, Yiwei Liu, Yueying Zhang, Yongxin Jin, Fang Bai, Zhihui Cheng, Shouguang Jin, Weihui Wu
Pseudomonas aeruginosa causes various acute and chronic infections in human. Treatment with azithromycin (AZM) has been shown to benefit patients with chronic P. aeruginosa infections. By binding to the exit tunnel of 50S ribosome, AZM causes ribosome stalling and depletion of intracellular tRNA pool. It has been shown that AZM is able to kill stationary-phase P. aeruginosa cells and repress quorum sensing regulated virulence factors as well as swarming motility. In P. aeruginosa, PA5470 encodes a putative peptide chain release factor, whose expression is highly induced by macrolide antibiotics...
December 4, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29191704/microfouling-inhibition-of-human-nosocomial-pathogen-pseudomonas-aeruginosa-using-marine-cyanobacteria
#2
Felix LewisOscar, Chari Nithya, Sulaiman Ali Alharbi, Naiyf S Alharbi, Nooruddin Thajuddin
Pseudomonas aeruginosa is a Gram negative, opportunistic biofilm forming pathogenic bacterium which is developing as a serious problem worldwide. The pathogenicity of P. aeruginosa mainly depends upon biofilm and quorum sensing (QS) mechanism. Targeting biofilm and QS regulated factor will automatically reduce the pathogenicity of P. aeruginosa. Therefore it is compulsory to identify naturally derived biofilm and QS inhibitors against P. aeruginosa. In the present study Oscillatoria subuliformis, a marine cyanobacterium was used against the biofilm and QS of P...
November 27, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29183968/catch-bond-drives-stator-mechanosensitivity-in-the-bacterial-flagellar-motor
#3
Ashley L Nord, Emilie Gachon, Ruben Perez-Carrasco, Jasmine A Nirody, Alessandro Barducci, Richard M Berry, Francesco Pedaci
The bacterial flagellar motor (BFM) is the rotary motor that rotates each bacterial flagellum, powering the swimming and swarming of many motile bacteria. The torque is provided by stator units, ion motive force-powered ion channels known to assemble and disassemble dynamically in the BFM. This turnover is mechanosensitive, with the number of engaged units dependent on the viscous load experienced by the motor through the flagellum. However, the molecular mechanism driving BFM mechanosensitivity is unknown...
November 28, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29180634/transient-locking-of-the-hook-procures-enhanced-motility-to-flagellated-bacteria
#4
Ismaël Duchesne, Tigran Galstian, Simon Rainville
Flagellated bacteria often proliferate in inhomogeneous environments, such as biofilms, swarms and soil. In such media, bacteria are observed to move efficiently only if they can get out of "dead ends" by changing drastically their swimming direction, and even to completely reverse it. Even though these reorientations are ubiquitous, we have only recently begun to describe and understand how they happen. In the present work, we visualized the flagella of bacteria swimming in a soft agar solution. The surprising observation that the filaments do not rotate while being flipped from one side of the cell to the other suggests that reversals are driven directly by the motor rather than by the thrust created by the rotating filament...
November 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29176964/only-acyl-carrier-protein-1-acpp1-functions-in-pseudomonas-aeruginosa-fatty-acid-synthesis
#5
Jin-Cheng Ma, Yun-Qi Wu, Dan Cao, Wen-Bin Zhang, Hai-Hong Wang
The genome of Pseudomonas aeruginosa contains three open reading frames, PA2966, PA1869, and PA3334, which encode putative acyl carrier proteins, AcpP1, AcpP2, and AcpP3, respectively. In this study, we found that, although these apo-ACPs were successfully phosphopantetheinylated by P. aeruginosa phosphopantetheinyl transferase (PcpS) and all holo-forms of these proteins could be acylated by Vibrio harveyi acyl-ACP synthetase (AasS), only AcpP1 could be used as a substrate for the synthesis of fatty acids, catalyzed by P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29169395/genome-wide-analysis-of-e-coli-cell-gene-interactions
#6
S Cardinale, G Cambray
BACKGROUND: The pursuit of standardization and reliability in synthetic biology has achieved, in recent years, a number of advances in the design of more predictable genetic parts for biological circuits. However, even with the development of high-throughput screening methods and whole-cell models, it is still not possible to predict reliably how a synthetic genetic construct interacts with all cellular endogenous systems. This study presents a genome-wide analysis of how the expression of synthetic genes is affected by systematic perturbations of cellular functions...
November 23, 2017: BMC Systems Biology
https://www.readbyqxmd.com/read/29157822/antibiofilm-activities-of-norharmane-and-its-derivatives-against-escherichia-coli-o157-h7-and-other-bacteria
#7
Jin-Hyung Lee, Yong-Guy Kim, Sang Hee Shim, Jintae Lee
BACKGROUND: Bacterial biofilms exhibit reduced sensitivity to conventional antibiotics and host defence systems and contribute to the persistence of chronic bacterial infections. HYPOTHESIS: The antibiofilm approach using plant alkaloids provides an alternative to antibiotic strategies. STUDY DESIGN: In this study, the antibiofilm activities of various plant alkaloids were investigated against enterohemorrhagic Escherichia coli O157:H7 and Pseudomonas aeruginosa...
December 1, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29151649/role-of-the-toxr-gene-from-fish-pathogen-vibiro-alginolyticus-in-the-physiology-and-virulence
#8
Shuanghu Cai, Haiyan Cheng, Huanying Pang, Yishan Lu, Jichan Jian
A mutant strain of Vibiro alginolyticus with an in-frame deletion of the toxR gene was constructed to reveal the role of ToxR in the physiology and virulence of V. alginolyticus. The statistical analysis showed no significant difference in the growth ability, swarming motility, activity of extracellular protease and the virulence by injection (the value of LD50) between the wild-type and the toxR mutant. However, the deletion of toxR could decrease the level of biofilm formation. The comparative proteomic analysis demonstrated the deletion mutation of toxR could up-regulate the expression of glutamine synthetase and levansucrase, and down-regulate the expression of 10 proteins such as OmpU, DnaK, etc...
December 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29146500/motility-biofilm-formation-apoptotic-effect-and-virulence-gene-expression-of-atypical-salmonella-typhimurium-outside-and-inside-caco-2-cells
#9
Ibtissem Chakroun, Abdelkarim Mahdhi, Patricia Morcillo, Hector Cordero, Alberto Cuesta, Amina Bakhrouf, Kacem Mahdouani, M Ángeles Esteban
Disease outbreaks related to waterborne pathogen contamination throughout the world as well as challenges that lie ahead for addressing persistent infection are of renewed interest. In this research, we studied the effects of prolonged exposure of Salmonella enterica serovar Typhimurium to the cues encountered in the extracellular environment particularly in seawater microcosm on bacterial virulence and subsequent infection in Caco-2 cells. Our data show a significant difference in biofilm formation, swimming and swarming motilities between normal and stressed cells of S...
November 13, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29142162/chloroform-extract-of-turmeric-inhibits-biofilm-formation-eps-production-and-motility-in-antibiotic-resistant-bacteria
#10
Sumreen Hayat, Anjum N Sabri, Timothy D McHugh
In the form of biofilms, bacteria exhibit more resistance to antibiotics. Biofilm formers can withstand severe conditions and the host's defense system. Therefore, it is necessary to search for effective biofilm inhibitors. In this study, we investigated the effect of a chloroform extract of turmeric on biofilm formation against antibiotic resistant bacteria. The extract exhibited its antibiofilm effect by altering adherence, motility, extracellular polymeric substance (EPS) production and cell surface hydrophobicity; important attributes of biofilm formation...
November 15, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/29141156/plasmid-azobr_p1-borne-fabg-gene-for-putative-3-oxoacyl-acyl-carrier-protein-reductase-is-essential-for-proper-assembly-and-work-of-the-dual-flagellar-system-in-the-alphaproteobacterium-azospirillum-brasilense-sp245
#11
Yulia A Filip'echeva, Andrei V Shelud'ko, Alexei G Prilipov, Gennady L Burygin, Elizaveta M Telesheva, Stella S Yevstigneyeva, Marina P Chernyshova, Lilia P Petrova, Elena I Katsy
Azospirillum brasilense can swim and swarm owing to the activity of a constitutive polar flagellum (Fla) and inducible lateral flagella (Laf), respectively. Experimental data on the regulation of the Fla and Laf assembly in azospirilla are scarce. Here, the coding sequence (CDS) AZOBR_p1160043 (fabG1) for a putative 3-oxoacyl-[acyl-carrier protein (ACP)] reductase was found essential for the construction of both types of flagella. In an immotile leaky Fla- Laf- fabG1::Omegon-Km mutant, Sp245.1610, defects in flagellation and motility were fully complemented by expressing the CDS AZOBR_p1160043 from plasmid pRK415...
November 15, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/29139003/differential-roles-for-arca-and-arcb-homologues-in-swarming-motility-in-serratia-marcescens-fs14
#12
Xiaolan Zhang, Defeng Wu, Tengfei Guo, Tingting Ran, Weiwu Wang, Dongqing Xu
ArcAB is a two-component regulatory system that can help bacteria respond to and survive in a changing environment. To identify the function of ArcAB homologues in Serratia marcescens FS14, in-frame deletion mutants of the arcA, arcB and arcAB genes were constructed. Surprisingly, ArcB affects the motility of FS14, but ArcA does not. These results are the reverse of those found in Escherichia coli. Further studies demonstrated that ArcB could promote bacterial motility by activating the synthesis of flagella and particularly by activating the expression of the biosurfactant serrawettin W1...
November 14, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29124040/cold-shock-domain-family-proteins-csps-are-involved-in-regulation-of-virulence-cellular-aggregation-and-flagella-based-motility-in-listeria-monocytogenes
#13
Athmanya K Eshwar, Claudia Guldimann, Anna Oevermann, Taurai Tasara
Cold shock-domain family proteins (Csps) are highly conserved nucleic acid binding proteins regulating the expression of various genes including those involved in stress resistance and virulence in bacteria. We show here that Csps are involved in virulence, cell aggregation and flagella-based extracellular motility of Listeria monocytogenes. A L. monocytogenes mutant deleted in all three csp genes (ΔcspABD) is attenuated with respect to human macrophage infection as well as virulence in a zebrafish infection model...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29104932/multidrug-resistant-salmonella-enterica-serovar-typhimurium-isolates-are-resistant-to-antibiotics-that-influence-their-swimming-and-swarming-motility
#14
Brian W Brunelle, Bradley L Bearson, Shawn M D Bearson, Thomas A Casey
Motile bacteria employ one or more methods for movement, including darting, gliding, sliding, swarming, swimming, and twitching. Multidrug-resistant (MDR) Salmonella carries acquired genes that provide resistance to specific antibiotics, and the goal of our study was to determine how antibiotics influence swimming and swarming in such resistant Salmonella isolates. Differences in motility were examined for six MDR Salmonella enterica serovar Typhimurium isolates grown on swimming and swarming media containing subinhibitory concentrations of chloramphenicol, kanamycin, streptomycin, or tetracycline...
November 2017: MSphere
https://www.readbyqxmd.com/read/29101490/inhibition-of-quorum-sensing-dependent-biofilm-and-virulence-genes-expression-in-environmental-pathogen-serratia-marcescens-by-petroselinic-acid
#15
Srinivasan Ramanathan, Durgadevi Ravindran, Kannappan Arunachalam, Veera Ravi Arumugam
The aim of this study was to evaluate the anti-biofilm and anti-virulence properties of petroselinic acid (PSA) against the environmental pathogen Serratia marcescens. PSA significantly inhibited the quorum sensing (QS)-dependent virulence factors such as prodigiosin, protease productions, and biofilm formation in S. marcescens. The antibiofilm potential of PSA was also confirmed through light, confocal laser scanning, and scanning electron microscopic analyses. Furthermore, PSA effectively inhibited the biofilm-related phenomena such as exopolysaccharide production, hydrophobicity production, swimming, and swarming motility without affecting the bacterial growth...
November 3, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29091745/h-ns-represses-transcription-of-the-flagellin-gene-lafa-of-lateral-flagella-in-vibrio-parahaemolyticus
#16
Yan Wang, Zhang Yiquan, Zhe Yin, Jie Wang, Yongzhe Zhu, Haoran Peng, Dongsheng Zhou, Zhongtian Qi, Wenhui Yang
Swarming motility is ultimately mediated by the proton-powered lateral flagellar (laf) system in Vibrio parahaemolyticus. Expression of laf is tightly regulated by a number of environmental conditions and regulatory factors. The nucleoid-associated DNA-binding protein H-NS is a small and abundant protein that is widely distributed in bacteria, and H-NS like proteins-dependent expression of laf genes have been identified in V. cholerae and V. parahaemolyticus. The data presented here shows that H-NS acts as a repressor of the swarming motility in V...
November 1, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/29075242/biocontrol-of-bacterial-fruit-blotch-by-bacillus-subtilis-9407-via-surfactin-mediated-antibacterial-activity-and-colonization
#17
Haiyan Fan, Zhanwei Zhang, Yan Li, Xun Zhang, Yongming Duan, Qi Wang
In this study, Bacillus subtilis 9407 showed a strong antibacterial activity against Acidovorax citrulli in vitro and 61.7% biocontrol efficacy on melon seedlings 4 days post inoculation under greenhouse conditions. To understand the biocontrol mechanism of B. subtilis 9407, identify the primary antibacterial compound and determine its role in controlling bacterial fruit blotch (BFB), a srfAB deletion mutant (ΔsrfAB) was constructed. The ΔsrfAB which was deficient in production of surfactin, not only showed almost no ability to inhibit growth of A...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29066541/pseudomonas-syringae-pv-syringae-b728a-regulates-multiple-stages-of-plant-colonization-via-the-bacteriophytochrome-bphp1
#18
Regina McGrane, Gwyn A Beattie
Light may be an important environmental signal for plant-associated bacteria, particularly those that live on leaves. An integrated network of red/far-red- and blue-light-responsive photosensory proteins is known to inhibit swarming motility in the foliar plant pathogen Pseudomonas syringae pv. syringae B728a. Here we elucidated factors in the red/far-red-light-sensing bacteriophytochrome BphP1 signal transduction pathway and report evidence for a role of BphP1 in multiple stages of the Psyringae B728a life cycle...
October 24, 2017: MBio
https://www.readbyqxmd.com/read/29061709/genomic-and-transcriptomic-analysis-of-escherichia-coli-strains-associated-with-persistent-and-transient-bovine-mastitis-and-the-role-of-colanic-acid
#19
John D Lippolis, Devin B Holman, Brian W Brunelle, Tyler C Thacker, Bradley L Bearson, Timothy A Reinhardt, Randy E Sacco, Thomas A Casey
Escherichia coli is a leading cause of bacterial mastitis in dairy cattle. It is most often transient in nature, causing an infection that lasts 2--3 days. However, E. coli has been shown to cause a persistent infection in a minority of cases. Mechanisms that allow for a persistent E. coli infection are not fully understood. The goal of this work was to determine differences between E. coli strains originally isolated from dairy cattle with transient and persistent mastitis. Using RNA sequencing, we show gene expression differences in nearly 200 genes when comparing bacteria from the two clinical phenotypes...
October 23, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/29061663/swrd-ylzi-promotes-swarming-in-bacillus-subtilis-by-increasing-power-to-flagellar-motors
#20
Ashley N Hall, Sundharraman Subramanian, Reid T Oshiro, Alexandra K Canzoneri, Daniel B Kearns
The bacterium Bacillus subtilis is capable of two methods of flagellar mediated motility: swimming, which occurs in liquid, and swarming, which occurs on a surface. Swarming is distinct from swimming in that it requires secretion of a surfactant, an increase in flagellar density, and perhaps additional factors. Here we report a new gene, swrD, encoded within the 32 gene fla/che operon dedicated to flagellar biosynthesis and chemotaxis, which when mutated abolished swarming motility. SwrD was not required for surfactant production, flagellar gene expression, or an increase in flagellar number...
October 23, 2017: Journal of Bacteriology
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