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https://www.readbyqxmd.com/read/28527130/pulse-generation-in-the-quorum-machinery-of-pseudomonas-aeruginosa
#1
Cicik Alfiniyah, Martin A Bees, A Jamie Wood
Pseudomonas aeruginosa is a Gram-negative bacterium that is responsible for a wide range of infections in humans. Colonies employ quorum sensing (QS) to coordinate gene expression, including for virulence factors, swarming motility and complex social traits. The QS signalling system of P. aeruginosa is known to involve multiple control components, notably the las, rhl and pqs systems. In this paper, we examine the las system and, in particular, the repressive interaction of rsaL, an embedded small regulative protein, employing recent biochemical information to aid model construction...
May 19, 2017: Bulletin of Mathematical Biology
https://www.readbyqxmd.com/read/28522292/cross-talk-between-bacterial-two-component-systems-drives-stepwise-regulation-of-flagellar-biosynthesis-in-swarming-development
#2
Chia-Fong Wei, Yu-Huan Tsai, Sheng-Hui Tsai, Chuan-Sheng Lin, Chih-Jung Chang, Chia-Chen Lu, Hsiou-Chen Huang, Hsin-Chih Lai
Swarming motility is a mode of bacterial movement over a solid surface driven by rotating flagella in a coordinated manner. Bacteria can use two-component system (TCS), which typically comprises a sensor kinase and a specific cognate response regulator, to properly react to environmental changes. We previously showed that the TCS RssAB suppresses flagellar biosynthesis master regulator flhDC specifically in swarming lag phase to control surface migration timing without affecting expansion rate in Serratia marcescens swarming development...
May 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28513418/flagellar-expression-in-clinical-isolates-of-non-typeable-haemophilus-influenzae
#3
Alejandro Carabarin-Lima, Patricia Lozano-Zarain, Miguel Castañeda-Lucio, Claudia Fabiola Martínez de la Peña, Julieta Martinez-Garcia, Norarizbeth Lara Flores, Elías Campos de la Cruz, Sirenia González-Posos, Rosa Del Carmen Rocha-Gracia
PURPOSE: Haemophilus influenzae is a commensal organism found in the upper respiratory tract of humans. When H. influenzae becomes a pathogen, these bacteria can move out of their commensal niche and cause multiple respiratory tract diseases such as otitis media, sinusitis, conjunctivitis and bronchitis in children, and chronic obstructive pulmonary disease in adults. However, H. influenzae is currently considered a non-flagellate bacterium. METHODOLOGY AND RESULTS: In this study, 90 clinical isolates of H...
May 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28504816/virulence-factors-and-antimicrobial-resistance-in-environmental-strains-of-vibrio-alginolyticus
#4
Marcos F Hernández-Robles, Ana K Álvarez-Contreras, Patricia Juárez-García, Iván Natividad-Bonifacio, Everardo Curiel-Quesada, Carlos Vázquez-Salinas, Elsa I Quiñones-Ramírez
Vibrio alginolyticus has acquired increasing importance because this microorganism may be pathogenic to aquatic animals and humans. It has been reported that some V. alginolyticus strains carry virulence genes derived from pathogenic V. cholerae and V. parahaemolyticus strains. In this work V. alginolyticus was isolated from oyster samples acquired from a food-market in Mexico City. Thirty isolates were identified as V. alginolitycus. Strains showed β-haemolysis and proteolytic activity and produced a capsule...
December 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28500044/rpon-%C3%AF-54-is-required-for-floc-formation-but-not-for-extracellular-polysaccharide-biosynthesis-in-a-floc-forming-aquincola-tertiaricarbonis-strain
#5
Dianzhen Yu, Ming Xia, Liping Zhang, Yulong Song, You Duan, Tong Yuan, Minjie Yao, Liyou Wu, Chunyuan Tian, Zhenbin Wu, Xiangzhen Li, Jizhong Zhou, Dongru Qiu
Some bacteria are capable of forming flocs in which bacterial cells are self-flocculated by secreted extracellular polysaccharides and other biopolymers. The floc-forming bacteria play a central role in activated sludge, which has been widely utilized for the treatment of municipal sewage and industrial wastewater. Here we use a floc-forming bacterium, Aquincola tertiaricarbonis RN12, as a model to explore the biosynthesis of EPS and the regulation of floc-formation. A large gene cluster for exopolysaccharide biosynthesis and a gene encoding the alternative sigma factor RpoN1, one of the four paralogues, have been identified in the floc-formation-deficient mutants generated by transposon mutagenesis and the gene functions have been further confirmed by genetic complementation analyses...
May 12, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28484444/leaf-extracts-of-mangifera-indica-l-inhibit-quorum-sensing-regulated-production-of-virulence-factors-and-biofilm-in-test-bacteria
#6
Fohad M Husain, Iqbal Ahmad, Abdullah S Al-Thubiani, Hussein H Abulreesh, Ibrahim M AlHazza, Farrukh Aqil
Quorum sensing (QS) is a global gene regulatory mechanism in bacteria for various traits including virulence factors. Disabling QS system with anti-infective agent is considered as a potential strategy to prevent bacterial infection. Mangifera indica L. (mango) has been shown to possess various biological activities including anti-QS. This study investigates the efficacy of leaf extracts on QS-regulated virulence factors and biofilm formation in Gram negative pathogens. Mango leaf (ML) extract was tested for QS inhibition and QS-regulated virulence factors using various indicator strains...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28458663/h-ns-mutation-mediated-crispr-cas-activation-inhibits-phage-release-and-toxin-production-of-escherichia-coli-stx2-phage-lysogen
#7
Qiang Fu, Shiyu Li, Zhaofei Wang, Wenya Shan, Jingjiao Ma, Yuqiang Cheng, Hengan Wang, Yaxian Yan, Jianhe Sun
Shiga toxin-converting bacteriophages (Stx phages) carry the stx gene and convert nonpathogenic bacterial strains into Shiga toxin-producing bacteria. There is limited understanding of the effect that an Escherichia coli (E. coli) clustered regularly interspaced short palindromic repeats (CRISPR)-Cas adaptive immune system has on Stx phage lysogen. We investigated heat-stable nucleoid-structuring (H-NS) mutation-mediated CRISPR-Cas activation and its effect on E. coli Stx2 phage lysogen. The Δhns mutant (MG1655Δhns) of the E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28452529/effect-of-cell-aspect-ratio-on-swarming-bacteria
#8
Bella Ilkanaiv, Daniel B Kearns, Gil Ariel, Avraham Be'er
Swarming bacteria collectively migrate on surfaces using flagella, forming dynamic whirls and jets that consist of millions of individuals. Because some swarming bacteria elongate prior to actual motion, cell aspect ratio may play a significant role in the collective dynamics. Extensive research on self-propelled rodlike particles confirms that elongation promotes alignment, strongly affecting the dynamics. Here, we study experimentally the collective dynamics of variants of swarming Bacillus subtilis that differ in length...
April 14, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28439038/pseudomonas-aeruginosa-derived-rhamnolipids-and-other-detergents-modulate-colony-morphotype-and-motility-in-the-burkholderia-cepacia-complex
#9
Steve P Bernier, Courtney Hum, Xiang Li, George A O'Toole, Nathan Magarvey, Michael G Surette
Competitive interactions mediated by released chemicals (e.g. toxins) are prominent in multispecies communities, but their effect at subinhibitory concentrations on susceptible bacteria is poorly understood. Although Pseudomonas aeruginosa and species of the Burkholderia cepacia complex (Bcc) can exist together as a co-infection in cystic fibrosis airways, P. aeruginosa toxins can kill Bcc species in vitro Consequently, these bacteria become an ideal in vitro model system to study the impact of sublethal levels of toxins on the biology of typical susceptible bacteria such as the Bcc when exposed to P...
April 24, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28434877/one-enzyme-three-metabolites-shewanella-algae-controls-siderophore-production-via-the-cellular-substrate-pool
#10
Sina Rütschlin, Sandra Gunesch, Thomas Böttcher
Shewanella algae B516 produces avaroferrin, an asymmetric hydroxamate siderophore, which has been shown to inhibit swarming motility of Vibrio alginolyticus. We aimed to elucidate the biosynthesis of this siderophore and to investigate how S. algae coordinates the production of avaroferrin and its two symmetric counterparts. We reconstituted the reaction in vitro with the main enzyme AvbD and the putative biosynthetic precursors, and demonstrate that multispecificity of this enzyme results in the production of all three cyclic hydroxamate siderophores that were previously isolated as natural products from S...
May 18, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28424524/hqia-a-novel-quorum-quenching-enzyme-which-expands-the-ahl-lactonase-family
#11
Marta Torres, Stéphane Uroz, Rafael Salto, Laure Fauchery, Emilia Quesada, Inmaculada Llamas
The screening of a metagenomic library of 250,000 clones generated from a hypersaline soil (Spain) allowed us to identify a single positive clone which confers the ability to degrade N-acyl homoserine lactones (AHLs). The sequencing of the fosmid revealed a 42,318 bp environmental insert characterized by 46 ORFs. The subcloning of these ORFs demonstrated that a single gene (hqiA) allowed AHL degradation. Enzymatic analysis using purified HqiA and HPLC/MS revealed that this protein has lactonase activity on a broad range of AHLs...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28421612/prospective-bacterial-quorum-sensing-inhibitors-from-indian-medicinal-plant-extracts
#12
Bipransh Kumar Tiwary, Runu Ghosh, Saurav Moktan, Vivek Kumar Ranjan, Priyankar Dey, Dibakar Choudhury, Somit Dutta, Dipanwita Deb, Abhaya Prasad Das, Ranadhir Chakraborty
As virulence of many pathogenic bacteria is regulated by the phenomenon of quorum sensing (QS), the present study aimed to find the QS-inhibiting (QS-I) property (if any) in sixty-one Indian medicinal plants. The presence of QS-I compound in the leaf-extract was evaluated by its ability to inhibit production of pigment in Chromobacterium violaceum MTCC 2656 (violacein) and Pseudomonas aeruginosa MTCC 2297 (pyocyanin) or swarming of P. aeruginosa MTCC 2297. Extracts of three plants, Astilbe rivularis, Fragaria nubicola and Osbeckia nepalensis have shown a dose- dependent inhibition of violacein production with no negative effect on bacterial growth...
April 19, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28402888/influence-of-physical-effects-on-the-swarming-motility-of-pseudomonas-aeruginosa
#13
Alexander Yang, Wai Shing Tang, Tieyan Si, Jay X Tang
Many species of bacteria can spread over a moist surface via a particular form of collective motion known as "surface swarming". This form of motility is typically studied by inoculating bacteria on a gel formed by 0.4-1.5% agar, which contains essential nutrients for their growth and proliferation. Using Pseudomonas aeruginosa and its pili-less mutant, ΔPilA, we investigate physical factors that either facilitate or restrict the swarming motility, measured by the rate of increase in area covered by a spreading bacterial colony, i...
April 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28392408/3-amino-1-8-naphthalimide-a-structural-analog-of-the-anti-cholera-drug-virstatin-inhibits-chemically-biased-swimming-and-swarming-motility-in-vibrios
#14
Hongxia Wang, Anisia J Silva, Jorge A Benitez
A screen for inhibitors of Vibrio cholerae motility identified the compound 3-amino 1,8-naphthalimide (3-A18NI), a structural analog of the cholera drug virstatin. Similar to virstatin, 3-A18NI diminished cholera toxin production. In contrast, 3-A18NI impeded swimming and/or swarming motility of V. cholerae and V. parahemolyticus suggesting that it could target the chemotaxis pathway shared by the polar and lateral flagellar system of vibrios. 3-A18NI did not inhibit the expression of V. cholerae major flagellin FlaA or the assembly of its polar flagellum...
April 6, 2017: Microbes and Infection
https://www.readbyqxmd.com/read/28384054/virulence-of-pseudomonas-syringae-pv-tomato-dc3000-is-influenced-by-the-catabolite-repression-control-protein-crc
#15
Suma Chakravarthy, Bronwyn G Butcher, Yingyu Liu, Katherine D'Amico, Matthew Coster, Melanie J Filiatrault
Pseudomonas syringae infects diverse plant species and is widely used as a model system in the study of effector function and the molecular basis of plant diseases. Although the relationship between bacterial metabolism, nutrient acquisition, and virulence has attracted increasing attention in bacterial pathology, it is largely unexplored in P. syringae. The Crc (catabolite repression control) protein is a putative RNA-binding protein that regulates carbon metabolism as well as a number of other factors in the pseudomonads...
April 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28378143/calr-is-required-for-the-expression-of-t6ss2-and-the-adhesion-of-vibrio-parahaemolyticus-to-hela-cells
#16
Lingyu Zhang, George Osei-Adjei, Ying Zhang, He Gao, Wenhui Yang, Dongsheng Zhou, Xinxiang Huang, Huiying Yang, Yiquan Zhang
Vibrio parahaemolyticus expresses one major virulence determinant T6SS2, which is constituted into three putative operons, i.e., VPA1027-1024, VPA1043-1028, and VPA1044-1046. CalR, a LysR-type transcriptional regulator, was originally identified as a repressor of the swarming motility and T3SS1 gene expression. As shown in this study, CalR binds to the promoter-proximal region of each of the three operons to activate their transcription, and moreover, CalR activates the adhesion of V. parahaemolyticus to HeLa cells...
April 4, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28377931/inhibition-of-pseudomonas-aeruginosa-biofilm-formation-by-traditional-chinese-medicinal-herb-herba-patriniae
#17
Bo Fu, Qiaolian Wu, Minyan Dang, Dangdang Bai, Qiao Guo, Lixin Shen, Kangmin Duan
New antimicrobial agents are urgently needed to treat infections caused by drug-resistant pathogens and by pathogens capable of persisting in biofilms. The aim of this study was to identify traditional Chinese herbs that could inhibit biofilm formation of Pseudomonas aeruginosa, an important human pathogen that causes serious and difficult-to-treat infections in humans. A luxCDABE-based reporter system was constructed to monitor the expression of six key biofilm-associated genes in P. aeruginosa. The reporters were used to screen a library of 36 herb extracts for inhibitory properties against these genes...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28377235/facile-green-synthesis-of-baicalein-fabricated-gold-nanoparticles-and-their-antibiofilm-activity-against-pseudomonas-aeruginosa-pao1
#18
Jobina Rajkumari, Siddhardha Busi, Aneesh Cherukunnel Vasu, Palarajasekhar Reddy
Biofilm formation is one of the major problems associated with chronic diseases and also attributes for the antibiotic resistance in bacteria. In recent times nanoparticles have been utilized to improve the efficacy of the existing antimicrobial and anti-biofilm agents. The ease in functionalization of gold nanoparticles (AuNPs) makes them a potential carrier for antimicrobial agents. However, the use of physical or chemical methods of the production of nanoparticles is expensive, labour intensive and hazardous to ecosystem...
April 2, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28370665/induction-of-the-pho-regulon-and-polyphosphate-synthesis-against-spermine-stress-in-pseudomonas-aeruginosa
#19
Yu-Chih Peng, ChienYi Lu, Guoqing Li, Zehava Eichenbaum, Chung-Dar Lu
Growth of Pseudomonas aeruginosa on spermine requires a functional γ-glutamylpolyamine synthetase PauA2. Not only subjected to growth inhibition by spermine, the pauA2 mutant became more sensitive to β-lactam antibiotics in human serum. To explore PauA2 as a potential target of drug development, suppressors of the pauA2 mutant, which alleviated toxicity, were isolated from selection plates containing spermine. These suppressors share common phenotypic changes including delayed growth rate, retarded swarming motility, and pyocyanin overproduction...
March 29, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28318117/transcriptional-regulation-of-cpsq-mfpabc-and-mfpabc-by-calr%C3%A2-in-vibrio-parahaemolyticus
#20
He Gao, Lingyu Zhang, George Osei-Adjei, Wenhui Yang, Dongsheng Zhou, Xinxiang Huang, Huiying Yang, Zhe Yin, Yiquan Zhang
The cpsQ-mfpABC locus is transcribed as two operons, i.e., cpsQ-mfpABC and mfpABC, in Vibrio parahaemolyticus, and both of them are all required for biofilm formation. CalR belongs to the LysR-type transcriptional regulator family, and was originally identified as a repressor of the swarming motility and T3SS1 genes expression in V. parahaemolyticus. In the present work, a combination of qRT-PCR, primer extension, LacZ fusion expression, electrophoretic mobility shift assay, and DNase I footprinting assays were employed to elucidate the regulatory mechanisms of cpsQ-mfpABC and mfpABC by CalR...
March 20, 2017: MicrobiologyOpen
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