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https://www.readbyqxmd.com/read/28535292/adaptive-radiation-of-p-fluorescens-sbw25-in-experimental-microcosms-provides-an-understanding-of-the-evolutionary-ecology-and-molecular-biology-of-a-l-interface-biofilm-formation
#1
Anna Koza, Anna Kusmierska, Kimberley McLaughlin, Olena Moshynets, Andrew J Spiers
Combined experimental evolutionary and molecular biology approaches have been used to investigate the adaptive radiation of Pseudomonas fluorescens SBW25 in static microcosms leading to the colonisation of the air-liquid interface by biofilm-forming mutants such as the Wrinkly Spreader. In these microcosms, the ecosystem engineering of the early wild-type colonists establish the niche space for subsequent WS evolution and colonisation. Random WS mutations occurring in the developing population that de-regulate diguanylate cyclases and c-di-GMP homeostasis result in cellulose-based biofilms at the air-liquid interface...
May 23, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28534180/the-c-terminal-kinesin-motor-kifc1-may-participate-in-nuclear-reshaping-and-flagellum-formation-during-spermiogenesis-of-larimichthys-crocea
#2
Dan-Dan Zhang, Xin-Ming Gao, Yong-Qiang Zhao, Cong-Cong Hou, Jun-Quan Zhu
Spermatogenesis is a highly ordered process in the differentiation of male germ cells. Nuclear morphogenesis is one of the most fundamental cellular transformations to take place during spermatogenesis. These striking transformations from spermatogonia to spermatozoa are a result of phase-specific adaption of the cytoskeleton and its association with molecular motor proteins. KIFC1 is a C-terminal kinesin motor protein that plays an essential role in acrosome formation and nuclear reshaping during spermiogenesis in mammals...
May 23, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28534119/splenic-ly6c-hi-monocytes-contribute-to-adverse-late-post-ischemic-left-ventricular-remodeling-in-heme-oxygenase-1-deficient-mice
#3
Mateusz Tomczyk, Izabela Kraszewska, Krzysztof Szade, Karolina Bukowska-Strakova, Marco Meloni, Alicja Jozkowicz, Jozef Dulak, Agnieszka Jazwa
Heme oxygenase-1 (Hmox1) is a stress-inducible protein crucial in heme catabolism. The end products of its enzymatic activity possess anti-oxidative, anti-apoptotic and anti-inflammatory properties. Cardioprotective effects of Hmox1 were demonstrated in experimental models of myocardial infarction (MI). Nevertheless, its importance in timely resolution of post-ischemic inflammation remains incompletely understood. The aim of this study was to determine the role of Hmox1 in the monocyte/macrophage-mediated cardiac remodeling in a mouse model of MI...
July 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/28533556/evolution-of-th2-responses-characterization-of-il-4-13-in-sea-bass-dicentrarchus-labrax-l-and-studies-of-expression-and-biological-activity
#4
Valentina Stocchi, Tiehui Wang, Elisa Randelli, Massimo Mazzini, Marco Gerdol, Alberto Pallavicini, Chris J Secombes, Giuseppe Scapigliati, Francesco Buonocore
Th2 immunity is a primary host defence against metazoan pathogens and two of the important cytokines involved in this immune response in mammals are IL-4 and IL-13. Recently the origin and evolution of Th2 immune responses have been investigated in fish where a molecule with relatedness to both IL-4 and IL-13 is present, termed IL-4/13. Different IL-4/13 paralogues (IL-4/13 A and IL-4/13B) exist in teleost fish. In this paper, we have focused on the IL-4/13 isoforms found in the European sea bass (Dicentrarchus labrax L...
May 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28533212/primitive-auxin-response-without-tir1-and-aux-iaa-in-the-charophyte-alga-klebsormidium-nitens
#5
Kinuka Ohtaka, Koichi Hori, Yuri Kanno, Mitsunori Seo, Hiroyuki Ohta
The phytohormone auxin regulates many aspects of growth and development in land plants, but the origin and evolution of auxin signaling and response mechanisms remain largely unknown. Indeed, it remains to be investigated whether auxin-related pathways diverged before the emergence of land plants. To address this knowledge deficit, we analyzed auxin responses in the charophyte alga Klebsormidium nitens NIES-2285, whose ancestor diverged from a green algal ancestor during the evolution of land plants. This strain is the same as K...
May 22, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28530638/sodium-channel-nav1-9-mutations-associated-with-insensitivity-to-pain-dampen-neuronal-excitability
#6
Jianying Huang, Carlos G Vanoye, Alison Cutts, Y Paul Goldberg, Sulayman D Dib-Hajj, Charles J Cohen, Stephen G Waxman, Alfred L George
Voltage-gated sodium channel (NaV) mutations cause genetic pain disorders that range from severe paroxysmal pain to a congenital inability to sense pain. Previous studies on NaV1.7 and NaV1.8 established clear relationships between perturbations in channel function and divergent clinical phenotypes. By contrast, studies of NaV1.9 mutations have not revealed a clear relationship of channel dysfunction with the associated and contrasting clinical phenotypes. Here, we have elucidated the functional consequences of a NaV1...
May 22, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28528197/ubiquitous-chromatin-opening-elements-ucoes-applications-in-biomanufacturing-and-gene-therapy
#7
REVIEW
Jonathan J Neville, Joe Orlando, Kimberly Mann, Bethany McCloskey, Michael N Antoniou
Ubiquitous Chromatin-opening Elements (UCOEs) are defined by their ability to consistently confer stable, site of integration-independent transgene expression that is proportional to copy number, including from within regions of heterochromatin such as centromeres. UCOEs structurally consist of methylation-free CpG islands encompassing single or dual divergently-transcribed housekeeping gene promoters. Since their discovery in 1999, UCOEs and their sub-fragments have found applications in areas of biotechnology requiring stable, reproducible, and high levels of gene expression...
May 17, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28527813/enhancer-evolution-and-the-origins-of-morphological-novelty
#8
REVIEW
Mark Rebeiz, Miltos Tsiantis
A central goal of evolutionary biology is to understand the genetic origin of morphological novelties-i.e. anatomical structures unique to a taxonomic group. Elaboration of morphology during development depends on networks of regulatory genes that activate patterned gene expression through transcriptional enhancer regions. We summarize recent case studies and genome-wide investigations that have uncovered diverse mechanisms though which new enhancers arise. We also discuss how these enhancer-originating mechanisms have clarified the history of genetic networks underlying diversification of genital structures in flies, limbs and neural crest in chordates, and plant leaves...
May 18, 2017: Current Opinion in Genetics & Development
https://www.readbyqxmd.com/read/28526851/characterization-of-trpa-channels-in-the-starfish-patiria-pectinifera-involvement-of-thermally-activated-trpa1-in-thermotaxis-in-marine-planktonic-larvae
#9
Shigeru Saito, Gen Hamanaka, Narudo Kawai, Ryohei Furukawa, Jun Gojobori, Makoto Tominaga, Hiroyuki Kaneko, Yoko Satta
The vast majority of marine invertebrates spend their larval period as pelagic plankton and are exposed to various environmental cues. Here we investigated the thermotaxis behaviors of the bipinnaria larvae of the starfish, Patiria pectinifera, in association with TRPA ion channels that serve as thermal receptors in various animal species. Using a newly developed thermotaxis assay system, we observed that P. pectinifera larvae displayed positive thermotaxis toward high temperatures, including toward temperatures high enough to cause death...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28526847/odorant-binding-proteins-expression-patterns-in-recently-diverged-species-of-anastrepha-fruit-flies
#10
Emeline Boni Campanini, Carlos Congrains, Felipe Rafael Torres, Reinaldo Alves de Brito
We studied two species of closely related South American fruit flies, Anastrepha fraterculus and Anastrepha obliqua which, despite being able to interbreed, still show some ecological and reproductive differences. Because part of these differences, such as host and mate preferences, may be related to olfactory perception, we focused our investigation on the differential expression of Odorant-binding protein (OBP) gene family, which participate in initial steps of the olfactory signal transduction cascade. We investigated patterns of expression of eight OBP genes by qPCR in male and female head tissues of both species...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28526050/dbcamp-regulates-adipogenesis-in-sheep-inguinal-preadipocytes
#11
Delin Kong, Jingjing Cui, Juncai Fu
BACKGROUND: The cyclic adenosine-monophosphate (cAMP) pathway is generally recognized as one of the essential pathways for the adipose conversion of rodent preadipocytes in vitro. However, divergent effects of cAMP on adipocyte differentiation have also been reported. Since there is very little data on non-rodent preadipose cells, the aim of the present work was to analyze the effects of one classic activator (dbcAMP) of the cAMP pathway on the proliferation and differentiation of sheep preadipocytes grown...
May 19, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28516231/molecular-evolution-of-type-ii-mage-genes-from-ancestral-maged2-gene-and-their-phylogenetic-resolution-of-basal-mammalian-clades
#12
Marcos De Donato, Sunday O Peters, Tanveer Hussain, Hectorina Rodulfo, Bolaji N Thomas, Masroor E Babar, Ikhide G Imumorin
Type II melanoma-associated antigens (MAGE) are a subgroup of about a dozen proteins found in various locations in the genome and expressed in normal tissues, thus are not related to cancer as the type I MAGE genes. This gene family exists as a single copy in non-mammals and monotremata, but found as two copies in metatherians and occur as a diverse group in all eutherians. Our studies suggest MAGED2 as the ancestor of this subfamily and the most likely evolutionary history of eutherian type II MAGE genes is hereby proposed based on synteny conservation, phylogenetic relations, genome location, homology conservation, and the protein and gene structures...
May 17, 2017: Mammalian Genome: Official Journal of the International Mammalian Genome Society
https://www.readbyqxmd.com/read/28515730/analysis-of-mads-box-gene-family-reveals-conservation-in-floral-organ-abcde-model-of-moso-bamboo-phyllostachys-edulis
#13
Zhanchao Cheng, Wei Ge, Long Li, Dan Hou, Yanjun Ma, Jun Liu, Qingsong Bai, Xueping Li, Shaohua Mu, Jian Gao
Mini chromosome maintenance 1, agamous, deficiens, and serum response factor (MADS)-box genes are transcription factors which play fundamental roles in flower development and regulation of floral organ identity. However, till date, identification and functions of MADS-box genes remain largely unclear in Phyllostachys edulis. In view of this, we performed a whole-genome survey and identified 34 MADS-box genes in P. edulis, and based on phylogeny, they were classified as MIKC(C), MIKC(∗), Mα, and Mβ. The detailed analysis about gene structure and motifs, phylogenetic classification, comparison of gene divergence and duplication are provided...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28512676/different-fermentation-processes-produced-variants-of-an-anti-cd52-monoclonal-antibody-that-have-divergent-in-vitro-and-in-vivo-characteristics
#14
Chao Zhuang, Chen Zheng, Yantian Chen, Zheng Huang, Yanchao Wang, Qiang Fu, Chen Zeng, Tong Wu, Liming Yang, Nianmin Qi
The anti-CD52 antibody has already been approved for the treatment of patients with resistant chronic lymphocytic leukemia, relapsing-remitting multiple sclerosis, and has demonstrable efficacy against stem cell transplantation rejection. A CHO cell line expressing a humanized anti-CD52 monoclonal antibody (mAb-TH) was cultivated in both fed-batch and perfusion modes, and then purified. The critical quality attributes of these mAb variants were characterized and the pharmacokinetics (PK) properties were investigated...
May 16, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28512462/de-novo-transcriptome-profiling-of-flowers-flower-pedicels-and-pods-of-lupinus-luteus-yellow-lupine-reveals-complex-expression-changes-during-organ-abscission
#15
Paulina Glazinska, Waldemar Wojciechowski, Milena Kulasek, Wojciech Glinkowski, Katarzyna Marciniak, Natalia Klajn, Jacek Kesy, Jan Kopcewicz
Yellow lupine (Lupinus luteus L., Taper c.), a member of the legume family (Fabaceae L.), has an enormous practical importance. Its excessive flower and pod abscission represents an economic drawback, as proper flower and seed formation and development is crucial for the plant's productivity. Generative organ detachment takes place at the basis of the pedicels, within a specialized group of cells collectively known as the abscission zone (AZ). During plant growth these cells become competent to respond to specific signals that trigger separation and lead to the abolition of cell wall adhesion...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28512242/a-systematic-analysis-of-oncogenic-gene-fusions-in-primary-colon-cancer
#16
Wigard P Kloosterman, Robert R J Coebergh van den Braak, Mark Pieterse, Markus J van Roosmalen, Anieta M Sieuwerts, Christina Stangl, Ronne Brunekreef, Zarina S Lalmahomed, Salo Ooft, Anne van Galen, Marcel Smid, Armel Lefebvre, Fried Zwartkruis, John W M Martens, John A Foekens, Katharina Biermann, Marco J Koudijs, Jan N M IJzermans, Emile E Voest
Genomic rearrangements that give rise to oncogenic gene fusions can offer actionable targets for cancer therapy. Here we present a systematic analysis of oncogenic gene fusions among a clinically well-characterized, prospectively collected set of 278 primary colon cancers spanning diverse tumor stages and clinical outcomes. Gene fusions and somatic genetic variations were identified in fresh frozen clinical specimens by Illumina RNA-sequencing, the STAR fusion gene detection pipeline, and GATK RNA-seq variant calling...
May 16, 2017: Cancer Research
https://www.readbyqxmd.com/read/28512107/divergent-requirements-for-ezh1-in-heart-development-versus-regeneration
#17
Shanshan Ai, Xianhong Yu, Yumei Li, Yong Peng, Chen Li, Yanzhu Yue, Ge Tao, Chuan-Yun Li, William T Pu, Aibin He
Rationale: Polycomb repressive complex 2 (PRC2) is a major epigenetic repressor that deposits methylation on histone H3 on lysine 27 (H3K27me) and controls differentiation and function of many cells, including cardiac myocytes. EZH1 and EZH2 are two alternative catalytic subunits with partial functional redundancy. The relative roles of EZH1 and EZH2 in heart development and regeneration are unknown. Objective: We compared the roles of EZH1 versus EZH2 in heart development and neonatal heart regeneration. Methods and Results: Heart development was normal in Ezh1(-/-) (E1KO) and Ezh2(f/f)::cTNT(-Cre) (E2KO) embryos...
May 16, 2017: Circulation Research
https://www.readbyqxmd.com/read/28511724/production-of-a-recombinant-swollenin-from-trichoderma-harzianum-in-escherichia-coli-and-its-potential-synergistic-role-in-biomass-degradation
#18
Clelton A Santos, Jaire A Ferreira-Filho, Anthonia O'Donovan, Vijai K Gupta, Maria G Tuohy, Anete P Souza
BACKGROUND: Fungal swollenins (SWOs) constitute a class of accessory proteins that are homologous to canonical plant expansins. Expansins and expansin-related proteins are well known for acting in the deagglomeration of cellulose structure by loosening macrofibrils. Consequently, SWOs can increase the accessibility and efficiency of the other enzymes involved in the saccharification of cellulosic substrates. Thus, SWOs are promising targets for improving the hydrolysis of plant biomass and for use as an additive to enhance the efficiency of an enzyme cocktail designed for the production of biofuels...
May 16, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28508704/differential-expression-of-genes-related-to-glucose-metabolism-in-domesticated-pigs-and-wild-boar
#19
Dafang He, Jideng Ma, Keren Long, Xun Wang, Xuewei Li, Anan Jiang, Mingzhou Li
Glucose metabolism is a basic biological process that shows substantial variation within and between species. Using pig as a model organism, we investigated differences in glucose metabolic genes in seven tissues from domesticated pigs (Rongchang pig and Tibetan pig, meanwhile, the Tibetan pig just as a special case of the domesticated pig under plateau condition) and wild boar. We found large differences in the expression of genes involved in multiple aspects of glucose metabolism, including genes associated with glucose transport, gluconeogenesis, and glycolysis...
May 16, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28506210/sucrose-non-ferment-1-related-protein-kinase-2-snrk2-genes-could-mediate-the-stress-responses-in-potato-solanum-tuberosum-l
#20
Jiangping Bai, Juan Mao, Hongyu Yang, Awais Khan, Aqi Fan, Siyan Liu, Junlian Zhang, Di Wang, Huijuan Gao, Jinlin Zhang
BACKGROUND: The SnRKs (sucrose non-fermenting 1 related protein kinase) are a gene family coding for Ser/Thr protein kinases and play important roles in linking the tolerance and metabolic responses of plants to abiotic stresses. To date, no genome-wide characterization of the sucrose non-ferment 1 related protein kinase 2 (SnRK2) subfamily has been conducted in potato (Solanum tuberosum L.). RESULTS: In this study, eight StSnRK2 genes (StSnRK2.1- StSnRK2.8) were identified in the genome of the potato (Solanum tuberosum L...
May 15, 2017: BMC Genetics
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