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https://www.readbyqxmd.com/read/28712894/antimicrobial-and-immunomodulatory-activity-of-host-defense-peptides-clavanins-and-ll-37-in-vitro-an-endodontic-perspective
#1
Stella Maris F Lima, Mirna S Freire, Ana Luisa O Gomes, Ana Paula C Cantuária, Flávia Rodrigues P Dutra, Beatriz S Magalhães, Maurício Gonçalves C Sousa, Ludovico Migliolo, Jeeser A Almeida, Octávio L Franco, Taia Maria B Rezende
Endodontic treatment is mainly based on root canal disinfection and its failure may be motivated by microbial resistance. Endodontic therapy can be benefitted by host defense peptides (HDPs), which are multifunctional molecules that act against persistent infection and inflammation. This study aimed to evaluate the antimicrobial, cytotoxic and immunomodulatory activity of several HDPs, namely clavanin A, clavanin A modified (MO) and LL-37, compared to intracanal medication Ca(OH)2. HDPs and Ca(OH)2 were evaluated by: (1) antimicrobial assays against Candida albicans and Enterococcus faecalis, (2) cytotoxicity assays and (3) cytokine tumor necrosis factor (TNF)-α, monocyte chemoattractant protein (MCP)-1, interleukin (IL)-1α, IL-6, IL-10 and IL-12 and nitric oxide (NO) production by RAW 264...
July 14, 2017: Peptides
https://www.readbyqxmd.com/read/28688091/erratum-to-clinical-comparison-of-the-effectiveness-of-7-and-14-day-intracanal-medications-in-root-canal-disinfection-and-inflammatory-cytokines
#2
Frederico C Martinho, Cinthya C Gomes, Gustavo G Nascimento, Ana P M Gomes, Fábio R M Leite
No abstract text is available yet for this article.
July 7, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28674723/preference-and-usage-of-intracanal-medications-during-endodontic-treatment
#3
Ahmad A Madarati, Muhammad S Zafar, Aya M N Sammani, Ayman O Mandorah, Hamzah A Bani-Younes
OBJECTIVES: To investigate the preferences of general dental practitioners (GDPs) and endodontists in using endodontic intra-canal medications (ICMs). METHODS: This observational and descriptive study was conducted in 2014 in the western province of Saudi Arabia. Following ethical clearance and 2 pilot studies, a web-based questionnaire was electronically sent to 375 randomly and systematically selected GDPs and all endodontists in the western province (n=49). An accompanying e-mail explained the study's aims and confirmed that the data yielded would remain confidential...
July 2017: Saudi Medical Journal
https://www.readbyqxmd.com/read/28673496/optimization-of-tube-current-in-cone-beam-computed-tomography-for-the-detection-of-vertical-root-fractures-with-different-intracanal-materials
#4
Hugo Gaêta-Araujo, Gabriela Queiroz Silva de Souza, Deborah Queiroz Freitas, Christiano de Oliveira-Santos
INTRODUCTION: There is no consensus about the accuracy of cone-beam computed tomography (CBCT) for detecting vertical root fractures (VRFs), nor is there certainty about the isolated effect of different tube current parameters on the diagnosis of VRF through CBCT scans. This study aimed to evaluate how tube current affects the detection of VRF on CBCT examinations in the absence of intracanal materials and in the presence of gutta-percha (GP) and metal (MP) or fiberglass (FP) intracanal posts...
June 30, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28654211/cleaning-lateral-morphological-features-of-the-root-canal-the-role-of-streaming-and-cavitation
#5
J P Robinson, R G Macedo, B Verhaagen, M Versluis, P R Cooper, L W M van der Sluis, A D Walmsley
AIM: To investigate the effects of ultrasonic activation file type, lateral canal location and irrigant on the removal of a biofilm-mimicking hydrogel from a fabricated lateral canal. Additionally, the amount of cavitation and streaming was quantified for these parameters. METHODOLOGY: An intracanal sonochemical dosimetry method was used to quantify the cavitation generated by an IrriSafe 25 mm length, size 25 file inside a root canal model filled with filtered degassed/saturated water or 3 different concentrations of NaOCl...
June 27, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28652899/comparison-of-the-antimicrobial-efficacy-of-calcium-hydroxide-and-photodynamic-therapy-against-enterococcus-faecalis-and-candida-albicans-in-teeth-with-periapical-lesions-an-in-vivo-study
#6
Zohre Ahangari, Maryam Mojtahed Bidabadi, Mohammad Asnaashari, Afsaneh Rahmati, Fahimeh Sadat Tabatabaei
Introduction: Elimination of pathological microflora of root canal systems is a major goal in endodontic treatment. This study aimed to compare the antimicrobial efficacy of calcium hydroxide as an intracanal medication and antibacterial photodynamic therapy (aPDT) against Enterococcus faecalis and Candida albicans in teeth with periapical (PA) lesions. Methods: This in vivo study was conducted on 20 patients with single-rooted mandibular premolar with previously failed endodontic treatment. This study was performed as a clinical trial (IRCTID: IRCT2016090429686N1)...
2017: Journal of Lasers in Medical Sciences
https://www.readbyqxmd.com/read/28638552/efficacy-of-three-methods-for-inserting-calcium-hydroxide-based-paste-in-root-canals
#7
Thales Galvão, Bernardo Camargo, Luciana Armada, Flávio Alves
BACKGROUND: To compare the quality of calcium hydroxide paste fillings performed by three different techniques. MATERIAL AND METHODS: Sixty extracted maxillary central incisors, with previous root canal treatment, were decoronated and the gutta-percha was completely removed from the root canals. Subsequently, the canals were filled with a calcium hydroxide-based paste composed of calcium hydroxide, bismuth carbonate, and glycerin. The study samples were divided into the following three groups on the basis of three insertion techniques (n = 20, each): conventional technique using a hand instrument (MAN), rotary Lentulo spiral (LEN) technique, and a combined technique combining conventional hand files with sonic activation through the EndoActivator device (EA)...
June 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28620636/a-scanning-electron-microscopic-evaluation-of-the-effectiveness-of-etidronic-acid-smearclear-and-mtad-in-removing-the-intracanal-smear-layer
#8
Hemant Kumar Yadav, Rakesh Kumar Yadav, Anil Chandra, Aseem Prakash Tikku
STATEMENT OF THE PROBLEM: Root canal therapy should not simply be the extirpation of the pulp and widening of the canal. But one should also focus on how to completely remove the loosely-attached smear layer because it has adverse effects on the final outcome of the treatment. PURPOSE: This study compared the efficacy of Etidronic acid, SmearClear and MTAD to remove the smear layer created during instrumentation in different regions of the root canal. MATERIALS AND METHOD: Fifty single-rooted mandibular premolars were decoronated from the cementoenamel junction and instrumented using the ProTaper universal rotary file system along with copious irrigation by 1...
June 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28589472/clinical-comparison-of-the-effectiveness-of-7-and-14-day-intracanal-medications-in-root-canal-disinfection-and-inflammatory-cytokines
#9
Frederico C Martinho, Cinthya C Gomes, Gustavo N Nascimento, Ana P M Gomes, Fábio R M Leite
OBJECTIVE: This clinical study compared the effectiveness of 7- and 14-day intracanal medications in the reduction of bacteria/endotoxins from primarily infected root canals and determined their antigenicity against macrophages through the levels of cytokines. METHODS: Seventy-two primarily infected teeth were randomly divided into six groups according to medication and time of application: 7-day groups = G1, Ca(OH)2 + saline solution (SSL); G2, Ca(OH)2 + 2% chlorhexidine (CHX) gel; and G3, 2% CHX gel and 14-day groups = G4, Ca(OH)2 + SSL; G5, Ca(OH)2 + 2% CHX gel; and G6, 2% CHX gel (all groups, n = 12)...
June 6, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28578888/comparative-evaluation-of-chemotactic-factor-effect-on-migration-and-differentiation-of-stem-cells-of-the-apical-papilla
#10
Sara Fayazi, Koyo Takimoto, Anibal Diogenes
INTRODUCTION: Cell homing strategies could potentially be used in regenerative endodontic procedures (REPs) to promote the progressive coronal migration of stem cells, including stem cells of the apical papilla (SCAPs), along with formation of a new vascular network without the need for intentional apical trauma and intracanal bleeding. Although many chemotactic factors have been investigated for different mesenchymal stem cells, their effect on SCAP migration and differentiation is not fully understood...
June 1, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28578886/antibacterial-properties-of-chitosan-nanoparticles-and-propolis-associated-with-calcium-hydroxide-against-single-and-multispecies-biofilms-an-in%C3%A2-vitro-and-in-situ-study
#11
Aldo Del Carpio-Perochena, Anil Kishen, Rafael Felitti, Anjali Y Bhagirath, Manoj R Medapati, Christopher Lai, Rodrigo S Cunha
INTRODUCTION: The aim of this study was to evaluate the efficacy of chitosan nanoparticles (CNPs) and ethanolic propolis extract (EPE) incorporated into a calcium hydroxide paste (Ca[OH]2) to kill bacterial biofilms. METHODS: Human root canal dentin was infected with Enterococcus faecalis for 21 days and also intraorally for 48 hours followed by incubation in brain-heart infusion for 48 hours to standardize biofilm growth. Ca(OH)2 pastes associated or not with CNPs or EPE were tested on biofilms for 7 and 14 days...
June 1, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28577961/histologic-evaluation-of-regenerative-endodontic-procedures-with-the-use-of-chitosan-scaffolds-in-immature-dog-teeth-with-apical-periodontitis
#12
Paulo J Palma, João C Ramos, João B Martins, Anibal Diogenes, Maria H Figueiredo, Paula Ferreira, Carlos Viegas, João M Santos
INTRODUCTION: The aim of this study was to evaluate histologically the newly formed tissues after regenerative endodontic procedures (REPs) in dogs using either a blood clot (BC) or 2 different formulations of a chitosan hydrogel as scaffolds. METHODS: Apical periodontitis was induced by inoculating immature teeth with oral plaque in 4 beagle dogs. Teeth (n = 96) were divided into 2 control (n = 20) and 4 test groups (n = 76) according to the treatment: apexification and REPs with BC, sodium hyaluronate:chitosan (HA:CS) scaffolds, or pectin:chitosan (P:CS) scaffolds...
May 31, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28571266/formulation-and-evaluation-of-sol-gel-drug-delivery-system-for-intracanal-ph-sensitive-controlled-delivery-of-chlorhexidine
#13
Kapil Gandhi, Radha Srikrishna Maganti, Harsimran Kaur, K S Vinod, Pratik Verma
INTRODUCTION: Persistence of viable micro-organisms even after thorough chemomechanical debridement has been cited as a major cause for endodontic failure. Chlorhexidine is a drug, which has shown marked efficacy against Enterococcus faecalis and Candida spp., which are mostly accounted for endodontic failure and it has demonstrated high degree of substantivity to dentin by adsorption. Another issue with chlorhexidine and other intracanal medicaments is the excessive or premature peaking of the drug leading to possible side effects...
April 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28545965/comparative-evaluation-of-micron-and-nano-sized-intracanal-medicaments-on-penetration-and-fracture-resistance-of-root-dentin-an-in-vitro-study
#14
Abburi Sireesha, R Jayasree, Sampath Vidhya, Sekar Mahalaxmi, V Sujatha, T S Sampath Kumar
Intracanal medicaments play a vital role in disinfection of root canal system. The aim of this study was to evaluate intratubular penetration and fracture resistance of roots filled with micron- and nano-sized intracanal medicaments: calcium hydroxide (CH), nanocalcium hydroxide (NCH), chitosan (CS) and nanochitosan (NCS). Their antibacterial effect on E. faecalis was tested using agar diffusion method. NCH and NCS were prepared by precipitation method and ionic crosslinking respectively. NCH and NCS particles were spherical, with an average particle size of 102±11...
May 22, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28527853/influence-of-intracanal-materials-in-vertical-root-fracture-pathway-detection-with-cone-beam-computed-tomography
#15
Kamile Leonardi Dutra, Camila Pachêco-Pereira, Eduardo Antunes Bortoluzzi, Carlos Flores-Mir, Manuel O Lagravère, Márcio Corrêa
INTRODUCTION: Investigating the vertical root fracture (VRF) pathway under different clinical scenarios may help to diagnose this condition properly. We aimed to determine the capability and intrareliability of VRF pathway detection through cone-beam computed tomographic (CBCT) imaging as well as analyze the influence of different intracanal and crown materials. METHODS: VRFs were mechanically induced in 30 teeth, and 4 clinical situations were reproduced in vitro: no filling, gutta-percha, post, and metal crown...
May 17, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28511520/effect-of-trauma-to-primary-tooth-on-permanent-maxillary-incisors-a-clinical-case-report
#16
Bhawana Gupta, Sachin Gupta, Jitesh Wadhwa, Alpa Gupta
Trauma to primary tooth leads to abnormal variations in crown and root canals of permanent teeth that presents a challenge in diagnosis and clinical management to the practitioner. This article presents a detailed case report of the endodontic treatment of a three canalled maxillary central incisor and maxillary lateral incisor having single canal with open apex linked with periodontal defect. Root canals were prepared with hand files and canals were irrigated with 2.5% sodium hypochlorite solution. Calcium hydroxide was placed as an intracanal medicament...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28494599/a-combined-approach-with-passive-and-active-repositioning-of-a-traumatically-intruded-immature-permanent-incisor
#17
H J Kim, Y I Kim, K S Min
BACKGROUND: Severe damage to the tooth, periodontal ligament, and pulpal tissue often occurs with intrusive luxation, and outcomes are quite unpredictable because of the variable ways in which the injury may occur, which influences both the choice of treatment and prognosis. CASE REPORT: This case presents a novel method for the management of an intruded permanent maxillary central incisor with an immature apex. At first, watchful waiting for spontaneous re-eruption was the chosen treatment option...
March 2017: European Journal of Paediatric Dentistry: Official Journal of European Academy of Paediatric Dentistry
https://www.readbyqxmd.com/read/28484231/effect-of-canal-anastomosis-on-periapical-fluid-pressure-build-up-during-needle-irrigation-in-single-roots-with-double-canals-using-a-polycarbonate-model
#18
Qi Huang, Jonathan B Barnes, G John Schoeffel, Bing Fan, Candice Tay, Brian E Bergeron, Lisiane F Susin, Jun-Qi Ling, Li-Na Niu, Franklin R Tay
Sodium hypochlorite is an effective irrigant for chemical debridement of root canals. However, increasing the intracanal pressure during irrigant delivery may result in irrigant extrusion into the bone and soft tissues surrounding the tooth. Because clinicians often encounter teeth with intracanal communications, the objective of the present study was to examine the effects of canal anastomosis on the generation of periapical fluid pressure at different fluid flow rates and insertion depths. Two similar polycarbonate models were used to simulate a single root with double canals, one containing, and the other without communicating channels between the canals...
May 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28483164/influence-of-the-apical-preparation-size-and-the-irrigant-type-on-bacterial-reduction-in-root-canal-treated-teeth-with-apical-periodontitis
#19
Renata Costa Val Rodrigues, Homan Zandi, Anne Karin Kristoffersen, Morten Enersen, Ibrahimu Mdala, Dag Ørstavik, Isabela N Rôças, José F Siqueira
INTRODUCTION: This clinical study evaluated the influence of the apical preparation size using nickel-titanium rotary instrumentation and the effect of a disinfectant on bacterial reduction in root canal-treated teeth with apical periodontitis. METHODS: Forty-three teeth with posttreatment apical periodontitis were selected for retreatment. Teeth were randomly divided into 2 groups according to the irrigant used (2.5% sodium hypochlorite [NaOCl], n = 22; saline, n = 21)...
May 5, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28475093/traumatic-periapical-lesion-only-identified-through-cone-beam-computed-tomography-a-case-report
#20
Leopoldo Cosme-Silva, Ana Flávia Oliveira Marcos, Júlia Fernandes Ferreira, João Eduardo Gomes Filho, Naiana Viana Viola
This case report describes the diagnosis of a traumatic periapical lesion, caused by parafunctional habits (bruxism) and associated with bone rarefaction, that had not been identified by periapical radiography. Slight edema was observed clinically at the apical region of the maxillary left canine; however, the tooth demonstrated only physiologic mobility, and the results of a pulpal sensitivity test were inconclusive. A cone beam computed tomogram (CBCT) showed the presence of a periapical lesion. After preparation of the root canal, intracanal medication (calcium hydroxide and paramonochlorophenol) was placed and changed once a month for 2 months...
May 2017: General Dentistry
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