Anastasiia Murmiliuk, Hiroki Iwase, Jia-Jhen Kang, Shilpa Mohanakumar, Marie-Sousai Appavou, Kathleen Wood, László Almásy, Adél Len, Kuno Schwärzer, Jürgen Allgaier, Martin Dulle, Thomas Gensch, Beate Förster, Kanae Ito, Hiroshi Nakagawa, Simone Wiegand, Stephan Förster, Aurel Radulescu
The co-assembly of polyelectrolytes (PE) with proteins offers a promising approach for designing complex structures with customizable morphologies, charge distribution, and stability for targeted cargo delivery. However, the complexity of protein structure limits our ability to predict the properties of the formed nanoparticles, and our goal is to identify the key triggers of the morphological transition in protein/PE complexes and evaluate their ability to encapsulate multivalent ionic drugs. A positively charged PE can assemble with a protein at pH above isoelectric point due to the electrostatic attraction and disassemble at pH below isoelectric point due to the repulsion...
March 23, 2024: Journal of Colloid and Interface Science