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https://www.readbyqxmd.com/read/29325751/a-difficult-diagnosis-of-coeliac-disease-repeat-duodenal-histology-increases-diagnostic-yield-in-patients-with-concomitant-causes-of-villous-atrophy
#1
Gaetano Cristian Morreale, Luigi Maria Montalbano, Maria Cappello, Emanuele Sinagra, Aroldo Rizzo, Antonio Carroccio
Villous atrophy in absence of coeliac disease (CD)-specific antibodies represents a diagnostic dilemma. We report a case of a woman with anaemia, weight loss and diarrhoea with an initial diagnosis of seronegative CD and a histological documented villous atrophy who did not improve on gluten-free diet due to the concomitant presence of common variable immunodeficiency (CVID) and Giardia lamblia infection. This case report confirms that CD diagnosis in CVID patients is difficult; the combination of anti-endomysial antibodies (EmA-IgA), anti-tissue transglutaminase antibodies (tTG-IgAb) antibodies and total IgA is obligatory in basic diagnostic of CD but in CVID are negative...
January 8, 2018: Arab Journal of Gastroenterology: the Official Publication of the Pan-Arab Association of Gastroenterology
https://www.readbyqxmd.com/read/29318125/prevalence-of-intestinal-parasites-in-food-handlers-of-bandar-abbas-southern-iran
#2
Mehrgan Heydari-Hengami, Yaghoob Hamedi, Majid Najafi-Asl, Khojasteh Sharifi-Sarasiabi
Background: Food handlers play a significant role in the dissemination and transmission of enteropathogenic microorganisms, including intestinal parasites. The aim of this study was to determine the prevalence of intestinal parasites in food handlers of Bandar Abbas, southern Iran. Methods: In this cross-sectional study, 800 stool samples were randomly collected in a period of 6 months from Jun to Nov 2015. The diagnosis was made on direct wet mount, formalin-ether concentration, Ziehl-Neelsen, and Trichrome stained slides...
January 2018: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29317877/phylogenetic-analysis-of-giardia-lamblia-human-genotypes-in-fars-province-southern-iran
#3
Mohammad Rayani, Gholamreza Hatam, Ngah Zasmy Unyah, Abdolmajid Ashrafmansori, Wan Omar Abdullah, Rukman Awang Hamat
Background: This study is the first phylogenetic genotype analysis of Giardia lamblia in Iran. The main objective was to determine genotyping and identify the sub-assemblages of Giardia lamblia isolates involved in the transmission of giardiasis in Fars Province, south of Iran, in 2012. Methods: Forty G. lamblia isolates were collected from the patient's fecal samples with gastrointestinal discomfort referred to the health centers and hospitals in Shiraz, Fars Province, south of Iran...
October 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/29314280/fabrication-of-silver-chloride-nanoparticles-using-a-plant-serine-protease-in-combination-with-photo-activation-and-investigation-of-their-biological-activities
#4
Jaruwan Siritapetawee, Wanwisa Limphirat, Nawarat Nantapong, Dujdow Songthamwat
Recently, the development of 'green' methods for fabrication of silver nanoparticles (Ag-NPs) has been emphasized, in view of their environmental safety, feasibility and low cost. In this study, a serine protease, EuP-82 from Euphorbia cf. lactea latex, was used to fabricate silver chloride nanoparticles (AgCl-NPs) in phosphate-buffered saline (pH 7.2), under the influence of visible light. The fabricated nanoparticles had a maximal surface plasmon resonance absorption peak at 435 nm. The size of the AgCl-NPs, estimated by scanning electron microscopy, was 57 ± 14...
January 3, 2018: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/29306723/current-prevalence-of-intestinal-parasitic-infections-and-their-impact-on-hematological-and-nutritional-status-among-karen-hill-tribe-children-in-omkoi-district-chiang-mai-province-thailand
#5
Jintana Yanola, Woottichai Nachaiwieng, Suwit Duangmano, Mujalin Prasannarong, Pradya Somboon, Sakorn Pornprasert
Intestinal parasitic infection represents a substantial problem for children living in rural or limited resources areas and significantly relates to anemia and nutritional status. This study aimed to determine the current prevalence of intestinal parasitic infections among school-age children of Karen hill tribe population in Omkoi District, Chiang Mai Province, Thailand and assess the impact of intestinal parasitic infection on hematological and nutritional status in those children. A total of 375 Karen hill tribe children, 6-14 years of age, in Omkoi District were randomly selected to participate in this study...
January 5, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29237648/parasitic-stool-testing-in-newly-arrived-refugees-in-calgary-alta
#6
Giselle DeVetten, Meghan Dirksen, Robert Weaver, Tanvir Turin Chowdhury, Michael William Aucoin
OBJECTIVE: To determine the prevalence of intestinal parasites and rates of stool testing compliance, as well as associated patient characteristics, among newly arrived refugees at the Mosaic Refugee Health Clinic in Calgary, Alta. DESIGN: Retrospective chart review. SETTING: Primary care clinic for refugee patients. PARTICIPANTS: A total of 1390 new refugee patients at the clinic from May 1, 2011, to June 30, 2013. MAIN OUTCOME MEASURES: Stool ova and parasite test completion and proportion of positive test results...
December 2017: Canadian Family Physician Médecin de Famille Canadien
https://www.readbyqxmd.com/read/29236304/enterovirus-detection-in-patients-with-acute-gastroenteritis-in-switzerland
#7
Rainer Gosert, Ulrich Heininger, Hans H Hirsch
Acute gastroenteritis (GE) has a major impact on morbidity and mortality worldwide, yet comprehensive data regarding infectious agents including enteroviruses are scarce. We hypothesized that enteroviruses constitute a significant cause of acute GE. We analyzed 677 stool samples from 504 patients, which had been submitted for suspected infectious GE. 0.2 ml of stool suspension was extracted using the Abbott m2000 sp robot and analysed by multiplex nucleic acid testing (NAT) using the Luminex xTAG gastrointestinal pathogen panel (GPP) as well as by specific NATs detecting enteroviruses and polioviruses...
December 13, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29224850/the-minimal-escrt-machinery-of-giardia-lamblia-has-altered-inter-subunit-interactions-within-the-escrt-ii-and-escrt-iii-complexes
#8
Nabanita Saha, Somnath Dutta, Shankari P Datta, Srimonti Sarkar
The ESCRT pathway functions at different subcellular membranes to induce their negative curvature, and it has been largely characterized in model eukaryotes belonging to Opisthokonta. But searches of the genomes of many nonopisthokonts belonging to various supergroups indicate that some of them may harbour fewer ESCRT components. Of the genomes explored thus far, one of the most minimal set of ESCRT components was identified in the human pathogen Giardia lamblia, which belongs to Excavata. Here we report that an ESCRT-mediated pathway most likely operates at the peripheral vesicles, which are located at the cell periphery and the bare zone of this protist...
December 6, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/29197728/disulfiram-as-a-novel-inactivator-of-giardia-lamblia-triosephosphate-isomerase-with-antigiardial-potential
#9
Adriana Castillo-Villanueva, Yadira Rufino-González, Sara-Teresa Méndez, Angélica Torres-Arroyo, Martha Ponce-Macotela, Mario Noé Martínez-Gordillo, Horacio Reyes-Vivas, Jesús Oria-Hernández
Giardiasis, the infestation of the intestinal tract by Giardia lamblia, is one of the most prevalent parasitosis worldwide. Even though effective therapies exist for it, the problems associated with its use indicate that new therapeutic options are needed. It has been shown that disulfiram eradicates trophozoites in vitro and is effective in vivo in a murine model of giardiasis; disulfiram inactivation of carbamate kinase by chemical modification of an active site cysteine has been proposed as the drug mechanism of action...
December 2017: International Journal for Parasitology, Drugs and Drug Resistance
https://www.readbyqxmd.com/read/29185612/intestinal-parasites-in-cancer-patients-in-the-south-of-brazil
#10
S Jeske, T F Bianchi, M Q Moura, B Baccega, N B Pinto, M E A Berne, M M Villela
Intestinal parasitic infections in immunocompromised patients can lead to serious complications when not diagnosed and treated early. This study aimed to investigate the frequency of intestinal parasites in cancer patients undergoing chemotherapy in the South of Brazil. Three fecal samples collected from each patient (73 individuals) were processed by Ritchie and Faust techniques and submitted to specific staining methods for intestinal protozoa. A 61.6% parasite and/or commensal positivity was found. Helminths identified were Ascaris lumbricoides (33...
November 27, 2017: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/29183355/intestinal-parasitosis-and-anaemia-among-patients-in-a-health-center-north-ethiopia
#11
Megbaru Alemu, Birhane Kinfe, Desalegn Tadesse, Wondemagegn Mulu, Tadesse Hailu, Endalew Yizengaw
OBJECTIVE: The aim of this cross-sectional study was to determine the magnitude of intestinal parasitosis and anaemia in a Health Center, North Ethiopia. RESULTS: A total of 427 outpatients were enrolled and the median age of the participants was 22 years. The prevalence of intestinal parasitosis was 143 (33.5%). Age, place of residence and occupation were significantly associated with intestinal parasitosis. When we see parasite specific factors, significant associations were observed for source of drinking water (P = 0...
November 28, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/29181192/selecting-pcr-for-the-diagnosis-of-intestinal-parasitosis-choice-of-targets-evaluation-of-in-house-assays-and-comparison-with-commercial-kits
#12
G N Hartmeyer, S V Hoegh, M N Skov, R B Dessau, M Kemp
Microscopy of stool samples is a labour-intensive and inaccurate technique for detection of intestinal parasites causing diarrhoea and replacement by PCR is attractive. Almost all cases of diarrhoea induced by parasites over a nine-year period in our laboratory were due to Giardia lamblia, Cryptosporidium species, or Entamoeba histolytica detected by microscopy. We evaluated and selected in-house singleplex real-time PCR (RT-PCR) assays for these pathogens in 99 stool samples from patients suspected of having intestinal parasitosis tested by microscopy...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/29165993/hybrid-structured-illumination-expansion-microscopy-reveals-microbial-cytoskeleton-organization
#13
Aaron R Halpern, Germain C M Alas, Tyler J Chozinski, Alexander R Paredez, Joshua C Vaughan
Recently developed tissue-hydrogel methods for specimen expansion now enable researchers to perform super-resolution microscopy with ~65 nm lateral resolution using ordinary microscopes, standard fluores-cent probes, and inexpensive reagents. Here we use the combination of specimen expansion and the optical super-resolution microscopy technique structured illumination microscopy (SIM) to extend the spatial reso-lution to ~30 nm. We apply this hybrid method, which we call ExSIM, to study the cytoskeleton of the im-portant human pathogen Giardia lamblia including the adhesive disc and flagellar axonemes...
November 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/29164041/prevalence-and-risk-factors-of-intestinal-parasite-infection-among-schoolchildren-in-the-peripheral-highland-regions-of-huanuco-peru
#14
Byungjin Choi, Bongyoung Kim
Objectives: Schoolchildren in developing countries are at greater risk of intestinal parasitic infections. This study aimed to estimate the prevalence and assess the risk factors of intestinal parasite infection among schoolchildren in rural areas of Peru. Methods: A volunteer team from the Korea International Cooperation Agency (KOICA) conducted a campaign for parasite eradication called "Chao parasitos" at five schools in the peripheral highland regions of Huanuco in October 2013...
October 2017: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/29138640/prevalence-of-soil-transmitted-helminthiases-and-schistosomiasis-in-preschool-age-children-in-mwea-division-kirinyaga-south-district-kirinyaga-county-and-their-potential-effect-on-physical-growth
#15
Stephen Sifuna Wefwafwa Sakari, Amos K Mbugua, Gerald M Mkoji
Intestinal parasitic infections can significantly contribute to the burden of disease, may cause nutritional and energetic stress, and negatively impact the quality of life in low income countries of the world. This cross-sectional study done in Mwea irrigation scheme, in Kirinyaga, central Kenya, assessed the public health significance of soil-transmitted helminthiases (STH), schistosomiasis, and other intestinal parasitic infections, among 361 preschool age children (PSAC) through fecal examination, by measuring anthropometric indices, and through their parents/guardians, by obtaining sociodemographic information...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29114494/giardiasis-mimicking-celiac-disease-in-a-patient-of-common-variable-immunodeficiency
#16
Kumar Saurabh, Vijaya Lakshmi Nag, Daisy Khera, Poonam Elhence
An adolescent boy presented to pediatrics outpatient department with complaints of recurrent diarrhea, nausea, vomiting, and pedal edema since 3-4 months, with no relief even after taking treatment. His investigation revealed decreased serum IgA, IgG, and IgM levels with hypoproteinemia. Duodenal biopsy showed features of celiac disease (CD), but tissue transglutaminase IgA was negative. In stool examination, plenty of Giardia lamblia cysts and eggs of Hymenolepis nana were present. He improved on treatment and remained asymptomatic for 4 months...
July 2017: Tropical Parasitology
https://www.readbyqxmd.com/read/29103276/intestinal-parasite-infections-among-inhabitants-in-yanbian-prefecture-jilin-province-china
#17
Myoung-Ro Lee, Hee-Eun Shin, Byung-Suk Chung, Sang-Eun Lee, Jung-Won Ju, Liji Xu, Chen Long Nan, Mi-Yeoun Park, Shin-Hyeong Cho
To investigate the prevalence of intestinal parasite infections in Yanbian Prefecture, Jilin Province, China, epidemiological surveys were conducted on a collaboration basis between the Korean Centers for Disease Control and Prevention and the Yanbian Center for Disease Control and Prevention. A total of 8,396 (males 3,737 and females 4,659) stool samples were collected from 8 localities and examined with the formalin-ether sedimentation technique, and additionally examined with the cellotape anal swab to detect Enterobius vermicularis eggs...
October 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29103267/intestinal-parasitic-infections-and-environmental-water-contamination-in-a-rural-village-of-northern-lao-pdr
#18
Alexis Ribas, Chloé Jollivet, Serge Morand, Boupha Thongmalayvong, Silaphet Somphavong, Chern-Chiang Siew, Pei-Jun Ting, Saipin Suputtamongkol, Viengsaene Saensombath, Surapol Sanguankiat, Boon-Huan Tan, Phimpha Paboriboune, Kongsap Akkhavong, Kittipong Chaisiri
A field survey studying intestinal parasites in humans and microbial pathogen contamination at environment was performed in a Laotian rural village to identify potential risks for disease outbreaks. A parasitological investigation was conducted in Ban Lak Sip village, Luang Prabang, Lao PDR involving fecal samples from 305 inhabitants as well as water samples taken from 3 sites of the local stream. Water analysis indicated the presence of several enteric pathogens, i.e., Aeromonas spp., Vibrio spp., E. coli H7, E...
October 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29099754/rnai-mediated-specific-gene-silencing-as-a-tool-for-the-discovery-of-new-drug-targets-in-giardia-lamblia-evaluation-using-the-nadh-oxidase-gene
#19
Jaime Marcial-Quino, Saúl Gómez-Manzo, Francisco Fierro, Yadira Rufino-González, Daniel Ortega-Cuellar, Edgar Sierra-Palacios, America Vanoye-Carlo, Abigail González-Valdez, Angélica Torres-Arroyo, Jesús Oria-Hernández, Horacio Reyes-Vivas
The microaerophilic protozoan Giardia lamblia is the agent causing giardiasis, an intestinal parasitosis of worldwide distribution. Different pharmacotherapies have been employed against giardiasis; however, side effects in the host and reports of drug resistant strains generate the need to develop new strategies that identify novel biological targets for drug design. To support this requirement, we have designed and evaluated a vector containing a cassette for the synthesis of double-stranded RNA (dsRNA), which can silence expression of a target gene through the RNA interference (RNAi) pathway...
November 3, 2017: Genes
https://www.readbyqxmd.com/read/29080954/microbiological-risk-infection-assessment-using-qmra-in-agriculture-systems-in-c%C3%A3-te-d-ivoire-west-africa
#20
Parfait K Kouamé, Hung Nguyen-Viet, Kouassi Dongo, Christian Zurbrügg, Jean Biémi, Bassirou Bonfoh
Poor wastewater management that results from a lack of appropriate sanitation infrastructure contributes to increasing health risks in urban areas in Côte d'Ivoire. We assessed the health risks associated with the use of wastewater for watering salad destined for human consumption, to help local authorities in developing appropriate risk mitigation measures for Yamoussoukro, the political capital of Côte d'Ivoire. We applied a stochastic approach based on quantitative microbiological risk assessment (QMRA), focusing on wastewater for farming activities and salad consumption at the household level...
October 28, 2017: Environmental Monitoring and Assessment
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