keyword
https://read.qxmd.com/read/38437216/air-liquid-interface-microfluidic-monitoring-sensor-platform-for-studying-autophagy-regulation-after-pm2-5-exposure
#1
JOURNAL ARTICLE
Lulu Zheng, Zhijin Yang, Zhiwei Xue, Mengya Chen, Yule Zhang, Shuqi Cai, Kejie Zheng, Bo Dai, Sixiu Liu, Songlin Zhuang, Guodong Sui, Dawei Zhang
Undoubtedly, a deep understanding of PM2.5 -induced tumor metastasis at the molecular level can contribute to improving the therapeutic effects of related diseases. However, the underlying molecular mechanism of fine particle exposure through long noncoding RNA (lncRNA) regulation in autophagy and, ultimately, lung cancer (LC) metastasis remains elusive; on the other hand, the related monitoring sensor platform used to investigate autophagy and cell migration is lacking. Herein, this study performed an air-liquid interface microfluidic monitoring sensor (AIMMS) platform to analyze human bronchial epithelial cells after PM2...
March 4, 2024: ACS Sensors
https://read.qxmd.com/read/38167306/inhibition-of-mir-542-3p-augments-autophagy-to-promote-diabetic-corneal-wound-healing
#2
JOURNAL ARTICLE
Danling Liao, Shijia Wei, Jianzhang Hu
BACKGROUND: Autophagy has recently been shown to be critical for protecting peripheral nerve regeneration. This study explored the impact of miR-542-3p on diabetic corneal nerve regeneration and epithelial healing through the regulation of autophagy. METHODS: A type 1 diabetes model was established in male mice through streptozotocin administration. Immunofluorescence staining of β-Tubulin III and sodium fluorescein staining were performed to observe corneal nerve fiber density and corneal epithelial healing, respectively...
January 3, 2024: Eye and Vision (London, England)
https://read.qxmd.com/read/38041431/polioencephalopathy-in-eurasier-dogs
#3
JOURNAL ARTICLE
Faye Rawson, Matthias Christen, Jeremy Rose, Emilie Paran, Tosso Leeb, Angela Fadda
BACKGROUND: Polioencephalopathies secondary to inborn errors of metabolism have been described in dogs, but few genetically characterized. OBJECTIVES: Clinically and genetically characterize polioencephalopathy in a family of Eurasier dogs. ANIMALS: Three Eurasier dogs (littermates) presented with early onset movement disorders (9 weeks in 2, 4-6 months in 1). Progressive gait abnormalities were detected in 2 of the dogs, persistent divergent strabismus in 1, whereas consciousness and behavior remained intact in all dogs...
December 2, 2023: Journal of Veterinary Internal Medicine
https://read.qxmd.com/read/37658069/three-dimensional-growth-sensitizes-breast-cancer-cells-to-treatment-with-ferroptosis-promoting-drugs
#4
JOURNAL ARTICLE
Sandhya Chipurupalli, Peijia Jiang, Xiaoyang Liu, Julia Linhares Santos, Paola Marcato, Kirill V Rosen
Drugs causing ferroptosis, iron-mediated cell death, represent promising tools for cancer treatment. While exploring the effect of these drugs on breast cancer (BC), we found that a ferroptosis-inducing drug erastin dramatically inhibits tumorigenicity of human BC cells in mice but when used at a concentration known to effectively kill other cell types only modestly reduces such growth in 2D monolayer culture. BCs grow in vivo as 3D masses, and we found that ferroptosis inducers erastin and sulfasalazine inhibit growth of multiple human BC cell lines in 3D culture significantly stronger than in 2D culture...
September 1, 2023: Cell Death & Disease
https://read.qxmd.com/read/37459465/analysis-of-atg4c-function-in-vivo
#5
JOURNAL ARTICLE
Isaac Tamargo-Gómez, Gemma G Martínez-García, María F Suárez, Pablo Mayoral, Gabriel Bretones, Aurora Astudillo, Jesús Prieto-Lloret, Christina Sveen, Antonio Fueyo, Nikolai Engedal, Carlos López-Otín, Guillermo Mariño
ATG4 (autophagy related 4 cysteine peptidase); ATG4A (autophagy related 4A cysteine peptidase); ATG4B (autophagy related 4B cysteine peptidase); ATG4C (autophagy related 4C cysteine peptidase); ATG4D (autophagy related 4D cysteine peptidase); Atg8 (autophagy related 8); GABARAP (GABA type A receptor-associated protein); GABARAPL1(GABA type A receptor-associated protein like 1); GABARAPL2 (GABA type A receptor-associated protein like 2); MAP1LC3A/LC3A (microtubule associated protein 1 light chain 3 alpha); MAP1LC3B/LC3B (microtubule associated protein 1 light chain 3 beta); mATG8 (mammalian Atg8); PE (phosphatidylethanolamine); PS (phosphatydylserine); SQSTM1/p62 (sequestosome 1)...
November 2023: Autophagy
https://read.qxmd.com/read/37373039/-atg4b-overexpression-extends-lifespan-and-healthspan-in-drosophila-melanogaster
#6
JOURNAL ARTICLE
Yongxuan Li, Wei Zhang, Yunshuang Ye, Yinan Sun, Liping Yang, Guijun Chen, Kangning Chen, Sheryl Smith, Jumin Zhou
Autophagy plays important but complex roles in aging, affecting health and longevity. We found that, in the general population, the levels of ATG4B and ATG4D decreased during aging, yet they are upregulated in centenarians, suggesting that overexpression of ATG4 members could be positive for healthspan and lifespan. We therefore analyzed the effect of overexpressing Atg4b (a homolog of human ATG4D ) in Drosophila , and found that, indeed, Atg4b overexpression increased resistance to oxidative stress, desiccation stress and fitness as measured by climbing ability...
June 8, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37125193/lncrna-dancr-restrained-the-survival-of-mycobacterium-tuberculosis-h37ra-by-sponging-mir-1301-3p-mir-5194
#7
JOURNAL ARTICLE
Yuliang Qu, Dan Jiang, Minjuan Liu, Hongxia Wang, Tao Xu, Haijin Zhou, Minlan Huang, Weitong Shu, Guangxian Xu
Tuberculosis is a worldwide contagion caused by Mycobacterium tuberculosis (MTB). MTB is characterized by intracellular parasitism and is semi-dormant inside host cells. The persistent inflammation caused by MTB can form a granuloma in lesion regions and intensify the latency of bacteria. In recent years, several studies have proven that long non-coding RNAs (lncRNAs) play critical roles in modulating autophagy. In our study, the Gene Expression Omnibus (GEO) databases were searched for lncRNAs that are associated with tuberculosis...
2023: Frontiers in Microbiology
https://read.qxmd.com/read/36765070/bi-allelic-atg4d-variants-are-associated-with-a-neurodevelopmental-disorder-characterized-by-speech-and-motor-impairment
#8
JOURNAL ARTICLE
Marie Morimoto, Vikas Bhambhani, Nour Gazzaz, Mariska Davids, Paalini Sathiyaseelan, Ellen F Macnamara, Jennifer Lange, Anna Lehman, Patricia M Zerfas, Jennifer L Murphy, Maria T Acosta, Camille Wang, Emily Alderman, Sara Reichert, Audrey Thurm, David R Adams, Wendy J Introne, Sharon M Gorski, Cornelius F Boerkoel, William A Gahl, Cynthia J Tifft, May Christine V Malicdan
Autophagy regulates the degradation of damaged organelles and protein aggregates, and is critical for neuronal development, homeostasis, and maintenance, yet few neurodevelopmental disorders have been associated with pathogenic variants in genes encoding autophagy-related proteins. We report three individuals from two unrelated families with a neurodevelopmental disorder characterized by speech and motor impairment, and similar facial characteristics. Rare, conserved, bi-allelic variants were identified in ATG4D, encoding one of four ATG4 cysteine proteases important for autophagosome biogenesis, a hallmark of autophagy...
February 10, 2023: NPJ Genomic Medicine
https://read.qxmd.com/read/36481429/heme-dependent-induction-of-mitophagy-program-during-differentiation-of-murine-erythroid-cells
#9
JOURNAL ARTICLE
Masatoshi Ikeda, Hiroki Kato, Hiroki Shima, Mitsuyo Matsumoto, Eijiro Furukawa, Yan Yan, Ruiqi Liao, Jian Xu, Akihiko Muto, Tohru Fujiwara, Hideo Harigae, Emery H Bresnick, Kazuhiko Igarashi
Although establishment and maintenance of mitochondria are essential for the production of massive amounts of heme in erythroblasts, mitochondria must be degraded upon terminal differentiation to red blood cells (RBCs), thus creating a biphasic regulatory process. Previously, we reported that iron deficiency in mice promotes mitochondrial retention in RBCs, suggesting that a proper amount of iron and/or heme is necessary for the degradation of mitochondria during erythroblast maturation. Because the transcription factor GATA1 regulates autophagy in erythroid cells, which involves mitochondrial clearance (mitophagy), we investigated the relationship between iron or heme and mitophagy by analyzing the expression of genes related to GATA1 and autophagy and the impact of iron or heme restriction on the amount of mitochondria...
February 2023: Experimental Hematology
https://read.qxmd.com/read/36464658/silica-nanoparticles-trigger-chaperone-hspb8-assisted-selective-autophagy-via-tfeb-activation-in-hepatocytes
#10
JOURNAL ARTICLE
Ye Zhu, Yukang Zhang, Zhuying Fan, Yuting Fang, Yucao Zheng, Yang Li, Man Yang, Caixia Guo, Yanbo Li, Xianqing Zhou, Zhiwei Sun, Ji Wang
Silica nanoparticles (SiNPs) are one of the most common inorganic nanomaterials. Autophagy is the predominant biological response to nanoparticles and transcription factor EB (TFEB) is a master regulator of the autophagy-lysosome pathway. Previous studies show that SiNPs induce autophagosome accumulation, yet the precise underlying mechanisms remain uncertain. The present study investigates the role of TFEB during SiNP-induced autophagy. SiNP-induced TFEB nuclear translocation is verified using immunofluorescence and western blot assay...
December 4, 2022: Small
https://read.qxmd.com/read/35859894/editorial-autophagy-mediated-cell-survival-and-death-in-disease-progression-and-treatment
#11
EDITORIAL
Yongqiang Chen, Yongchao Zhao, Paras Kumar Mishra
No abstract text is available yet for this article.
2022: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/35625868/butyrate-inhibits-colorectal-cancer-cell-proliferation-through-autophagy-degradation-of-%C3%AE-catenin-regardless-of-apc-and-%C3%AE-catenin-mutational-status
#12
JOURNAL ARTICLE
Beatrice Garavaglia, Letizia Vallino, Alessandra Ferraresi, Andrea Esposito, Amreen Salwa, Chiara Vidoni, Sergio Gentilli, Ciro Isidoro
Colorectal cancer (CRC) pathogenesis is mainly driven by alterations in WNT signaling, which results in altered transcriptional activity of β-Catenin. Mutations in APC (Adenomatous Polyposis Coli) are reflected in β-Catenin hyperactivation and loss of proliferation control. Certain intestinal bacteria metabolites have shown the ability to limit CRC cell proliferation and CRC pathogenesis. Here, we investigated the molecular mechanism underlying the anti-proliferative activity of butyrate, a microbiota-derived short chain fatty acid, in two CRC cell lines, namely HCT116 and SW620, which bear a mutation in β-Catenin and APC , respectively...
May 13, 2022: Biomedicines
https://read.qxmd.com/read/35348614/a-genome-wide-association-study-in-autoimmune-neurological-syndromes-with-anti-gad65-autoantibodies
#13
JOURNAL ARTICLE
Christine Strippel, Marisol Herrera-Rivero, Mareike Wendorff, Anja K Tietz, Frauke Degenhardt, Anika Witten, Christina Schroeter, Christopher Nelke, Kristin S Golombeck, Marie Madlener, Theodor Rüber, Leon Ernst, Attila Racz, Tobias Baumgartner, Guido Widman, Kathrin Doppler, Franziska Thaler, Kai Siebenbrodt, Andre Dik, Constanze Kerin, Saskia Räuber, Marco Gallus, Stjepana Kovac, Oliver M Grauer, Alexander Grimm, Harald Prüss, Jonathan Wickel, Christian Geis, Jan Lewerenz, Norbert Goebels, Marius Ringelstein, Til Menge, Björn Tackenberg, Christoph Kellinghaus, Christian G Bien, Andrea Kraft, Uwe Zettl, Fatme Seval Ismail, Ilya Ayzenberg, Christian Urbanek, Kurt-Wolfram Sühs, Simone C Tauber, Sigrid Mues, Peter Körtvélyessy, Robert Markewitz, Asterios Paliantonis, Christian E Elger, Rainer Surges, Claudia Sommer, Tania Kümpfel, Catharina C Gross, Holger Lerche, Jörg Wellmer, Carlos M Quesada, Florian Then Bergh, Klaus-Peter Wandinger, Albert J Becker, Wolfram S Kunz, Gerd Meyer Zu Hörste, Michael P Malter, Felix Rosenow, Heinz Wiendl, Gregor Kuhlenbäumer, Frank Leypoldt, Wolfgang Lieb, Andre Franke, Sven G Meuth, Monika Stoll, Nico Melzer
Autoimmune neurological syndromes (AINS) with autoantibodies against the 65 kDa isoform of the glutamic acid decarboxylase (GAD65) present with limbic encephalitis, including temporal lobe seizures or epilepsy, cerebellitis with ataxia, and stiff-person-syndrome or overlap forms. Anti-GAD65 autoantibodies are also detected in autoimmune diabetes mellitus, which has a strong genetic susceptibility conferred by human leukocyte antigen (HLA) and non-HLA genomic regions. We investigated the genetic predisposition in patients with anti-GAD65 AINS...
March 1, 2023: Brain
https://read.qxmd.com/read/34718852/lncrna-thril-promotes-high-glucose-induced-proliferation-and-migration-of-human-retina-microvascular-endothelial-cells-through-enhancing-autophagy
#14
JOURNAL ARTICLE
Qingshan Ji, Jing Han, Jiajia Liu, Huayi Lv, Lisong Wang, Yiran Dong, Lei Shi
AIMS: Diabetes retinopathy (DR) is associated with retinal microvascular system injury induced by high glucose (HG). This study aims to explore the role and mechanism of long non-coding RNA THRIL in regulating cell proliferation and migration of human retina microvascular endothelial cells (hRMECs) under HG condition. METHOD: The gene and protein expression were detetced by RT-PCR and western blot, respectively. Cell proliferation and migration of hRMECs were examined using MTT assay and Transwell assay, respectively...
October 30, 2021: Acta Diabetologica
https://read.qxmd.com/read/34706226/subtractive-crispr-screen-identifies-the-atg16l1-vacuolar-atpase-axis-as-required-for-non-canonical-lc3-lipidation
#15
JOURNAL ARTICLE
Rachel Ulferts, Elena Marcassa, Lewis Timimi, Liam Changwoo Lee, Andrew Daley, Beatriz Montaner, Suzanne Dawn Turner, Oliver Florey, John Kenneth Baillie, Rupert Beale
Although commonly associated with autophagosomes, LC3 can also be recruited to membranes by covalent lipidation in a variety of non-canonical contexts. These include responses to ionophores such as the M2 proton channel of influenza A virus. We report a subtractive CRISPR screen that identifies factors required for non-canonical LC3 lipidation. As well as the enzyme complexes directly responsible for LC3 lipidation in all contexts, we show the RALGAP complex is important for M2-induced, but not ionophore drug-induced, LC3 lipidation...
October 26, 2021: Cell Reports
https://read.qxmd.com/read/34694211/up-regulation-of-microrna-mir-101-3p-enhances-sensitivity-to-cisplatin-via-regulation-of-small-interfering-rna-sirna-anti-human-agt4d-and-autophagy-in-non-small-cell-lung-carcinoma-nsclc
#16
JOURNAL ARTICLE
Dong Cui, Yu Feng, Rulin Qian
The emergence of drug resistance hinders the treatment of malignant tumors, and autophagy plays an important role in tumor chemotherapy resistance. However, its mechanism in non-small cell lung cancer (NSCLC) has not been well-researched. We aim to investigate the role of miR-101-3p in cisplatin-resistant via regulation of autophagy-related protein 4D (ATG4D) and autophagy. Cell viability, apoptosis, fluorescence intensity of GFP-LC3 and RFP-GFP-LC3 were determined using Cell Counting Kit-8 (CCK-8) assay, flow cytometry, and Laser scanning confocal microscope analysis, respectively...
December 2021: Bioengineered
https://read.qxmd.com/read/34313895/silencing-of-atg4d-suppressed-proliferation-and-enhanced-cisplatin-induced-apoptosis-in-hepatocellular-carcinoma-through-akt-caspase-3-pathway
#17
JOURNAL ARTICLE
Jing-Yuan Zhao, Xiao-Yun Li, Tian-De Liu, Bo Liang, Yong Huang, Wen Li
ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. However, the role of ATG4D in hepatocellular carcinoma (HCC) has not been defined. Herein, this study aimed to investigate the role and the underlying mechanism of ATG4D in regulating HCC cell apoptosis. ATG4D was silenced in MHCC-97L HCC cells, and then cell proliferation and apoptosis were examined. ATG4D expression was significantly upregulated in HCC tissues when compared with paired non-tumor tissues...
July 27, 2021: Molecular and Cellular Biochemistry
https://read.qxmd.com/read/34238288/identification-of-autophagy-related-risk-signatures-for-the-prognosis-diagnosis-and-targeted-therapy-in-cervical-cancer
#18
JOURNAL ARTICLE
Dan Meng, Hua Jin, Xing Zhang, Wenjing Yan, Qianqian Xia, Siyuan Shen, Shuqian Xie, Mengjing Cui, Bo Ding, Yun Gu, Shizhi Wang
BACKGROUND: To rummage autophagy-related prognostic, diagnostic, and therapeutic biomarkers in cervical cancer (CC). METHODS: The RNA-sequence and clinical information were from the TCGA and GTEx databases. We operated Cox regression to determine signatures related to overall survival (OS) and recurrence-free survival (RFS) respectively. The diagnostic and therapeutic effectiveness of prognostic biomarkers were further explored. RESULTS: We identified nine (VAMP7, MTMR14, ATG4D, KLHL24, TP73, NAMPT, CD46, HGS, ATG4C) and three risk signatures (SERPINA1, HSPB8, SUPT20H) with prognostic values for OS and RFS respectively...
July 8, 2021: Cancer Cell International
https://read.qxmd.com/read/34195903/development-and-validation-of-a-ferroptosis-related-prognostic-model-in-pancreatic-cancer
#19
JOURNAL ARTICLE
Chen-Jie Qiu, Xue-Bing Wang, Zi-Ruo Zheng, Chao-Zhi Yang, Kai Lin, Kai Zhang, Min Tu, Kui-Rong Jiang, Wen-Tao Gao
BACKGROUND: The purpose of this study was to identify ferroptosis-related genes (FRGs) associated with the prognosis of pancreatic cancer and to construct a prognostic model based on FRGs. METHODS: Based on pancreatic cancer data obtained from The Cancer Genome Atlas database, we established a prognostic model from 232 FRGs. A nomogram was constructed by combining the prognostic model and clinicopathological features. Gene Expression Omnibus datasets and tissue samples obtained from our center were utilized to validate the model...
June 30, 2021: Investigational New Drugs
https://read.qxmd.com/read/33988247/pathogenic-variants-of-atg4d-in-infertile-men-with-non-obstructive-azoospermia-identified-using-whole-exome-sequencing
#20
JOURNAL ARTICLE
Yanwei Sha, Wensheng Liu, Xiaoli Wei, Xingshen Zhu, Bowen Tang, Xiaoya Zhang, Xiaoyu Yang, Yifeng Wang, Xiong Wang
Non-obstructive azoospermia (NOA) is the most severe form of male infertility, and it is primarily associated with genetic defects. We performed whole-exome sequencing of 236 patients with NOA and identified a homozygous pathogenic variant of autophagy-related 4D cysteine peptidase (ATG4D) in two siblings from a consanguineous family and compound heterozygous pathogenic variants of ATG4D in two sporadic cases. The expression of LC3B, a regulator of autophagic activity, was significantly decreased, and the apoptosis rate of spermatogenic cells in testicular tissues was increased...
September 2021: Clinical Genetics
keyword
keyword
117607
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.