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https://www.readbyqxmd.com/read/28610697/respiratory-motion-of-adrenal-gland-metastases-analyses-using-four-dimensional-computed-tomography-images
#1
Bing Chen, Yong Hu, Jin Liu, An-Ning Cao, Lu-Xi Ye, Zhao-Chong Zeng
PURPOSE: To evaluate the respiratory motion of adrenal gland metastases in three-dimensional directions using four-dimensional computed tomography (4DCT) images. METHODS: From January 2013 to May 2016, 12 patients with adrenal gland metastases were included in this study. They all underwent 4DCT scans to assess respiratory motion of adrenal gland metastases in free breathing state. The 4DCT images were sorted into 10 image series according to the respiratory phase from the end inspiration to the end expiration, and then transferred to FocalSim workstation...
June 2017: Physica Medica: PM
https://www.readbyqxmd.com/read/28585318/hermansky-pudlak-syndrome-type-2-aberrant-pre-mrna-splicing-and-mislocalization-of-granule-proteins-in-neutrophils
#2
Martin de Boer, Karin van Leeuwen, Judy Geissler, Floris van Alphen, Esther de Vries, Martijn van der Kuip, Suzanne W J Terheggen, Hans Janssen, Timo K van den Berg, Alexander B Meijer, Dirk Roos, Taco W Kuijpers
Hermansky-Pudlak syndrome type 2 (HPS2) is a syndrome caused by mutations in the beta-3A subunit of the adaptor protein (AP)-3 complex (AP3B1 gene). We describe five unreported cases with four novel mutations, one of which caused aberrant pre-mRNA splicing. A point mutation c.2702C>G in exon 23 of the AP3B1 gene caused deletion of 112 bp in the mRNA in two siblings. This mutation activates a cryptic donor splice site that overrules the wild-type donor splice site of this exon. Three other novel mutations in AP3B1 were identified, that is, a nonsense mutation c...
June 6, 2017: Human Mutation
https://www.readbyqxmd.com/read/28559361/ap3-adaptor-protein-dependent-vacuolar-trafficking-involves-a-subpopulation-of-copii-and-hops-tethering-proteins
#3
Qiang-Nan Feng, Shi-Jian Song, Shi-Xia Yu, Jia-Gang Wang, Sha Li, Yan Zhang
Plant vacuoles are versatile organelles critical for plant growth and responses to environment. Vacuolar proteins are transported from the endoplasmic reticulum (ER) via multiple routes in plants. Two classic routes bear great similarity to other phyla with major regulators known, such as COPII and Rab5 GTPases. By contrast, vacuolar trafficking mediated by adaptor protein-3 (AP-3) or that independent of Golgi has few recognized cargos and none of the regulators. In search of novel regulators for vacuolar trafficking routes and by using a fluorescence-based forward genetic screen, we demonstrated that the multi-span transmembrane protein, Arabidopsis PROTEIN S-ACYL TRANSFERASE 10 (PAT10), is an AP-3-mediated vacuolar cargo...
May 30, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28494164/effects-of-enhancing-vitamin-d-status-by-25-hydroxycholecalciferol-supplementation-alone-or-in-combination-with-calcium-and-phosphorus-on-sternum-mineralisation-and-breast-meat-quality-in-broilers
#4
M Bozkurt, S Yalçin, B Koçer, A E Tüzün, H Akşit, S Özkan, M Uygun, G Ege, G Güven, O Yildiz
1. The aim of the present study was to examine the effects of improving vitamin D status in broiler diets by supplementary 25-hydroxycholecalciferol (25OHD3), alone or in combination with calcium (Ca) and available phosphorus (aP), on live performance, sternum mineralisation and breast meat quality in broilers. 2. A total of 936 1-d-old Ross 308 broilers were used in the study. After gender determination at the hatchery, chicks from each sex were randomly distributed into three dietary treatments. The following dietary treatments were used in the experiment from hatch to 38 d: (1) A control diet formulated to meet all of the nutrient requirements of broiler chicks according to the management guide; (2) The control diet supplemented with 18...
June 15, 2017: British Poultry Science
https://www.readbyqxmd.com/read/28486943/kb-r7943-reduces-4-aminopyridine-induced-epileptiform-activity-in-adult-rats-after-neuronal-damage-induced-by-neonatal-monosodium-glutamate-treatment
#5
Mariana Hernandez-Ojeda, Monica E Ureña-Guerrero, Paola E Gutierrez-Barajas, Jazmin A Cardenas-Castillo, Antoni Camins, Carlos Beas-Zarate
BACKGROUND: Neonatal monosodium glutamate (MSG) treatment triggers excitotoxicity and induces a degenerative process that affects several brain regions in a way that could lead to epileptogenesis. Na(+)/Ca(2+) exchangers (NCX1-3) are implicated in Ca(2+) brain homeostasis; normally, they extrude Ca(2+) to control cell inflammation, but after damage and in epilepsy, they introduce Ca(2+) by acting in the reverse mode, amplifying the damage. Changes in NCX3 expression in the hippocampus have been reported immediately after neonatal MSG treatment...
May 9, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28382459/proteomic-studies-on-anti-tumor-agent-ansamitocin-p-3-producer-actinosynnema-pretiosum-in-response-to-ammonium-and-isobutanol
#6
Jinxia Lin, Jian-Jiang Zhong
Our previous work showed that the biosynthesis of ansamitocin P-3 (AP-3), an anti-tumor agent, by Actinosynnema pretiosum was depressed by ammonium but enhanced by isobutanol in the medium. Here we show proteomics analyses on A. pretiosum in different fermentation conditions with and without ammonium or isobutanol using two-dimensional electrophoresis (2-DE), matrix-assisted laser desorption/ionization, and linear ion trap quadrupole mass spectrometry. Pairwise comparison of repetitive 2-DE maps was performed to find differentially expressed spots, and eight proteins were identified as functionally annotated ones...
April 5, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28381425/an-endosomal-syntaxin-and-the-ap-3-complex-are-required-for-formation-and-maturation-of-candidate-lysosome-related-secretory-organelles-mucocysts-in-tetrahymena-thermophila
#7
Harsimran Kaur, Daniela Sparvoli, Hiroko Osakada, Masaaki Iwamoto, Tokuko Haraguchi, Aaron P Turkewitz
The ciliate Tetrahymena thermophila synthesizes large secretory vesicles called mucocysts. Mucocyst biosynthesis shares features with dense core granules (DCGs) in animal cells, including proteolytic processing of cargo proteins during maturation. However, other molecular features have suggested relatedness to lysosome-related organelles (LROs). LROs, which include diverse organelles in animals, are formed via convergence of secretory and endocytic trafficking. Here we analyzed Tetrahymena syntaxin 7-like 1 (Stx7l1p), a Qa-SNARE whose homologues in other lineages are linked with vacuoles/LROs...
June 1, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28295320/brefeldin-a-sensitive-mechanisms-contribute-to-endocytotic-membrane-retrieval-and-vesicle-recycling-in-cerebellar-granule-cells
#8
Alberto Rampérez, José Sánchez-Prieto, Magdalena Torres
The recycling of synaptic vesicle (SV) proteins and transmitter release occur at multiple sites along the axon. These processes are sensitive to inhibition of the small GTP binding protein ARF1, which regulates the adaptor protein 1 and 3 complex (AP-1/AP-3). As the axon matures, SV recycling becomes restricted to the presynaptic bouton, and its machinery undergoes a complex process of maturation. We used the styryl dye FM1-43 to highlight differences in the efficiency of membrane recycling at different sites in cerebellar granule cells cultured for 7 days in vitro...
March 11, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28273137/adaptor-protein-3-a-key-player-in-rbl-2h3-mast-cell-mediator-release
#9
Elaine Zayas Marcelino da Silva, Edismauro Garcia Freitas-Filho, Devandir Antonio de Souza-Júnior, Luis Lamberti Pinto daSilva, Maria Celia Jamur, Constance Oliver
Mast cell (MC) secretory granules are Lysosome-Related Organelles (LROs) whose biogenesis is associated with the post-Golgi secretory and endocytic pathways in which the sorting of proteins destined for a specific organelle relies on the recognition of sorting signals by adaptor proteins that direct their incorporation into transport vesicles. The adaptor protein 3 (AP-3) complex mediates protein trafficking between the trans-Golgi network (TGN) and late endosomes, lysosomes, and LROs. AP-3 has a recognized role in LROs biogenesis and regulated secretion in several cell types, including many immune cells such as neutrophils, natural killer cells, and cytotoxic T lymphocytes...
2017: PloS One
https://www.readbyqxmd.com/read/28266544/misrouting-of-v-atpase-subunit-v0a1-dysregulates-lysosomal-acidification-in-a-neurodegenerative-lysosomal-storage-disease-model
#10
Maria B Bagh, Shiyong Peng, Goutam Chandra, Zhongjian Zhang, Satya P Singh, Nagarajan Pattabiraman, Aiyi Liu, Anil B Mukherjee
Defective lysosomal acidification contributes to virtually all lysosomal storage disorders (LSDs) and to common neurodegenerative diseases like Alzheimer's and Parkinson's. Despite its fundamental importance, the mechanism(s) underlying this defect remains unclear. The v-ATPase, a multisubunit protein complex composed of cytosolic V1-sector and lysosomal membrane-anchored V0-sector, regulates lysosomal acidification. Mutations in the CLN1 gene, encoding PPT1, cause a devastating neurodegenerative LSD, INCL...
March 7, 2017: Nature Communications
https://www.readbyqxmd.com/read/28065385/processing-of-the-abasic-sites-clustered-with-the-benzo-a-pyrene-adducts-by-the-base-excision-repair-enzymes
#11
Lidia V Starostenko, Nadejda I Rechkunova, Natalia A Lebedeva, Alexander A Lomzov, Vladimir V Koval, Olga I Lavrik
The major enzyme in eukaryotic cells that catalyzes the cleavage of apurinic/apyrimidinic (AP or abasic) sites is AP endonuclease 1 (APE1) that cleaves the phosphodiester bond on the 5'-side of AP sites. We found that the efficiency of AP site cleavage by APE1 was affected by the benzo[a]pyrenyl-DNA adduct (BPDE-dG) in the opposite strand. AP sites directly opposite of the modified dG or shifted toward the 5' direction were hydrolyzed by APE1 with an efficiency moderately lower than the AP site in the control DNA duplex, whereas AP sites shifted toward the 3' direction were hydrolyzed significantly less efficiently...
February 2017: DNA Repair
https://www.readbyqxmd.com/read/28041699/impact-of-interventional-edge-to-edge-repair-on-mitral-valve-geometry
#12
Robert Schueler, Sarah Kaplan, Charlotte Melzer, Can Öztürk, Marcel Weber, Jan-Malte Sinning, Armin Welz, Nikos Werner, Georg Nickenig, Christoph Hammerstingl
BACKGROUND/OBJECTIVES: The acute and long-term effects of interventional edge-to-edge repair on the mitral valve (MV) geometry are unclear. We sought to assess MV-annular geometry and the association of changes in MV-diameters with functional response one year after MitraClip implantation. METHODS: Consecutive patients (n=84; age 81.2±8.3years, logistic EuroSCORE 21.7±17.9%) with symptomatic moderate-to-severe mitral regurgitation (MR) underwent MitraClip-procedure...
March 1, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/27904451/the-ratio-of-weight-loss-to-planning-target-volume-significantly-impacts-setup-errors-in-nasopharyngeal-cancer-patients-undergoing-helical-tomotherapy-with-daily-megavoltage-computed-tomography
#13
Wei-Hsien Hou, Chun-Wei Wang, Chiao-Ling Tsai, Feng-Ming Hsu, Jason Chia-Hsien Cheng
BACKGROUND: Changes in head and neck anatomy during radiation therapy (RT) produce setup uncertainties of nasopharyngeal cancer (NPC) irradiation. We retrospectively analyzed image guidance data to identify clinical predictors of setup errors. PATIENTS AND METHODS: The data of 217 NPC patients undergoing definitive RT on a helical tomotherapy (HT) unit were analyzed. Factors including tumor stage, body mass index, weight loss, and planning target volume (PTV) were assessed as predictors of daily megavoltage computed tomography (MVCT) setup displacements, which were automatically registered using software...
December 1, 2016: Radiology and Oncology
https://www.readbyqxmd.com/read/27900401/vaginal-wall-weakness-in-parous-ewes-a-potential-preclinical-model-of-pelvic-organ-prolapse
#14
Natharnia Young, Anna Rosamilia, John Arkwright, Joseph Lee, Miranda Davies-Tuck, Joan Melendez, Jerome Werkmeister, Caroline E Gargett
INTRODUCTION AND HYPOTHESIS: Ewes develop pelvic organ prolapse (POP) and may be a suitable model for preclinical studies evaluating cell-based therapies for POP. The aim of this study was to establish a clinical score of vaginal weakness and to compare POP Quantification System (POP-Q) values in conscious nulliparous and parous ewes and determine whether ewes are a suitable POP model. METHODS: Ewes (n = 114) were examined while conscious, without sedation, and standing in a V conveyer by adapting the human POP-Q measurement...
July 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/27889498/bloc-2-subunit-hps6-deficiency-affects-the-tubulation-and-secretion-of-von-willebrand-factor-from-mouse-endothelial-cells
#15
Jing Ma, Zhe Zhang, Lin Yang, Janos Kriston-Vizi, Daniel F Cutler, Wei Li
Hermansky-Pudlak syndrome (HPS) is a recessive disorder with bleeding diathesis, which has been linked to platelet granule defects. Both platelet granules and endothelial Weibel-Palade bodies (WPBs) are members of lysosome-related organelles (LROs) whose formation is regulated by HPS protein associated complexes such as BLOC (biogenesis of lysosome-related organelles complex) -1, -2, -3, AP-3 (adaptor protein complex-3) and HOPS (homotypic fusion and protein sorting complex). Von Willebrand factor (VWF) is critical to hemostasis, which is stored in a highly-multimerized form as tubules in the WPBs...
December 20, 2016: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/27889060/autosomal-recessive-mutations-in-ap3b2-adaptor-related-protein-complex-3-beta-2-subunit-cause-an-early-onset-epileptic-encephalopathy-with-optic-atrophy
#16
Mirna Assoum, Christophe Philippe, Bertrand Isidor, Laurence Perrin, Periklis Makrythanasis, Neal Sondheimer, Caroline Paris, Jessica Douglas, Gaetan Lesca, Stylianos Antonarakis, Hanan Hamamy, Thibaud Jouan, Yannis Duffourd, Stéphane Auvin, Aline Saunier, Amber Begtrup, Catherine Nowak, Nicolas Chatron, Dorothée Ville, Kamiar Mireskandari, Paolo Milani, Philippe Jonveaux, Guylène Lemeur, Mathieu Milh, Masano Amamoto, Mitsuhiro Kato, Mitsuko Nakashima, Noriko Miyake, Naomichi Matsumoto, Amira Masri, Christel Thauvin-Robinet, Jean-Baptiste Rivière, Laurence Faivre, Julien Thevenon
Early-onset epileptic encephalopathy (EOEE) represents a heterogeneous group of severe disorders characterized by seizures, interictal epileptiform activity with a disorganized electroencephalography background, developmental regression or retardation, and onset before 1 year of age. Among a cohort of 57 individuals with epileptic encephalopathy, we ascertained two unrelated affected individuals with EOEE associated with developmental impairment and autosomal-recessive variants in AP3B2 by means of whole-exome sequencing...
December 1, 2016: American Journal of Human Genetics
https://www.readbyqxmd.com/read/27787765/effects-of-the-methylmalonyl-coa-metabolic-pathway-on-ansamitocin-production-in-actinosynnema-pretiosum
#17
Mengjiang Zhao, Yuxiang Fan, Liujing Wei, Fengxian Hu, Qiang Hua
Ansamitocins, which may have antitumor activity, are important secondary metabolites produced by Actinosynnema pretiosum sp. auranticum ATCC 31565. As one of the precursors for ansamitocin biosynthesis, methylmalonyl-CoA may be a critical metabolic node for secondary metabolism in A. pretiosum. In this study, we investigated two key enzymes related to the methylmalonyl-CoA metabolic pathway: methylmalonyl-CoA mutase (MCM) and propionyl-CoA carboxylase (PCC). For MCM, inactivation of the asm2277 gene (encoding the large subunit of MCM) resulted in 3-fold increase in ansamitocin P-3 (AP-3) production (reaching 70 mg/L) compared with that in wild-type A...
March 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27776132/tyrosine-binding-protein-sites-regulate-the-intracellular-trafficking-and-processing-of-amyloid-precursor-protein-through-a-novel-lysosome-directed-pathway
#18
Joshua H K Tam, M Rebecca Cobb, Claudia Seah, Stephen H Pasternak
The amyloid hypothesis posits that the production of β-amyloid (Aβ) aggregates leads to neurodegeneration and cognitive decline associated with AD. Aβ is produced by sequential cleavage of the amyloid precursor protein (APP) by β- and γ-secretase. While nascent APP is well known to transit to the endosomal/ lysosomal system via the cell surface, we have recently shown that APP can also traffic to lysosomes intracellularly via its interaction with AP-3. Because AP-3 interacts with cargo protein via interaction with tyrosine motifs, we mutated the three tyrosines motif in the cytoplasmic tail of APP...
2016: PloS One
https://www.readbyqxmd.com/read/27713690/the-endosome-localized-arf-gap-agap1-modulates-dendritic-spine-morphology-downstream-of-the-neurodevelopmental-disorder-factor-dysbindin
#19
Miranda Arnold, Rebecca Cross, Kaela S Singleton, Stephanie Zlatic, Christopher Chapleau, Ariana P Mullin, Isaiah Rolle, Carlene C Moore, Anne Theibert, Lucas Pozzo-Miller, Victor Faundez, Jennifer Larimore
AGAP1 is an Arf1 GTPase activating protein that interacts with the vesicle-associated protein complexes adaptor protein 3 (AP-3) and Biogenesis of Lysosome Related Organelles Complex-1 (BLOC-1). Overexpression of AGAP1 in non-neuronal cells results in an accumulation of endosomal cargoes, which suggests a role in endosome-dependent traffic. In addition, AGAP1 is a candidate susceptibility gene for two neurodevelopmental disorders, autism spectrum disorder (ASD) and schizophrenia (SZ); yet its localization and function in neurons have not been described...
2016: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/27672626/local-reference-patient-dose-evaluation-in-conventional-radiography-examinations-in-mazandaran-iran
#20
D Khoshdel-Navi, A Shabestani-Monfared, M R Deevband, R Abdi, M Nabahati
BACKGROUND: The most efficient application of ionizing radiation is serving medical purposes and using this radiation has caused people to learn that artificial sources of radiation exposure among these resources can be of highest exposure rate. OBIECTIVE: The present study is aimed at initially establishing a baseline for local-reference dose level in Mazandaran, Iran in 12 projections of the most conventional x-ray examination. METHODS: In this study, 13 public hospitals in Mazandaran province were selected for review and required data collected for ten adult patients with mean weight of 70±10kg in each projection...
June 2016: Journal of Biomedical Physics & Engineering
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