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https://www.readbyqxmd.com/read/29034180/significance-of-fermentation-process-on-biochemical-properties-of-phaseolus-vulgaris-red-beans
#1
Anteneh Worku, Omprakash Sahu
Phaseolus vulgaris L. beans have been characterized as a nearly perfect food in all around the world. It is consider functional in terms of providing nutrients and energy to sustain daily life. Red bean contain high protein, mineral, fibres and chemically diverse micronutrient composition, which can be affected by processing. The main aim of this work is to investigate the effect of open and controlled fermentation on the proximate composition, mineral elements, antinutritional factors and flatulence- causing oligosaccharides of a domesticated bean (Anger, conscope and Loko)...
December 2017: Biotechnology Reports
https://www.readbyqxmd.com/read/29033911/characterization-and-exploitation-of-crispr-loci-in-bifidobacterium-longum
#2
Claudio Hidalgo-Cantabrana, Alexandra B Crawley, Borja Sanchez, Rodolphe Barrangou
Diverse CRISPR-Cas systems provide adaptive immunity in many bacteria and most archaea, via a DNA-encoded, RNA-mediated, nucleic-acid targeting mechanism. Over time, CRISPR loci expand via iterative uptake of invasive DNA sequences into the CRISPR array during the adaptation process. These genetic vaccination cards thus provide insights into the exposure of strains to phages and plasmids in space and time, revealing the historical predatory exposure of a strain. These genetic loci thus constitute a unique basis for genotyping of strains, with potential of resolution at the strain-level...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29031239/probiotic-intervention-influences-the-salivary-levels-of-matrix-metalloproteinase-mmp-9-and-tissue-inhibitor-of-metalloproteinases-timp-1-in-healthy-adults
#3
Heli Jäsberg, Taina Tervahartiala, Timo Sorsa, Eva Söderling, Anna Haukioja
OBJECTIVE: To study the effect of orally administered Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus rhamnosus GG on the salivary levels of Matrix Metalloproteinases (MMP)-8, MMP-9 and of Tissue Inhibitor of Metalloproteinases (TIMP)-1 in healthy adults. Furthermore, the correlations between MMP-8, MMP-9 and TIMP-1 and plaque and gingival indices, salivary mutans streptococci and lactobacilli counts, and stimulated saliva secretion rate were analysed. DESIGN: The salivary samples originated from a randomized controlled trial where healthy student volunteers consumed probiotic or placebo lozenges twice a day for four weeks...
October 7, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29026733/safety-of-a-new-synbiotic-starter-formula
#4
Yvan Vandenplas, Antonis Analitis, Chara Tziouvara, Athina Kountzoglou, Anastasia Drakou, Manos Tsouvalas, Antigoni Mavroudi, Ioannis Xinias
PURPOSE: Breastfeeding is the best way to feed all infants, but not all infants can be (exclusively) breastfed. Cow's milk based infant formula is the second choice infant feeding. METHODS: The safety of a new synbiotic infant formula, supplemented with Bifidobacterium lactis and fructo-oligosaccharides, with lactose and a whey/casein 60/40 protein ratio was tested in 280 infants during 3 months. RESULTS: The median age of the infants at inclusion was 0...
September 2017: Pediatric Gastroenterology, Hepatology & Nutrition
https://www.readbyqxmd.com/read/29026604/transglucosidase-improves-the-bowel-movements-in-type-2-diabetes-mellitus-patients-a-preliminary-randomized-double-blind-placebo-controlled-study
#5
Akihiro Shimozato, Makoto Sasaki, Naotaka Ogasawara, Yasushi Funaki, Masahide Ebi, Chiho Goto, Satoshi Koikeda, Takashi Joh, Kunio Kasugai
BACKGROUND: Recent studies have highlighted the relationship between gut microbiota and bowel movements. OBJECTIVE: We aimed to evaluate transglucosidase treatment efficacy for bowel movements in patients with type 2 diabetes mellitus and to clarify the relationship between bowel movements, dietary habits, gut microbiota and fecal short-chain fatty acids. METHODS: In this randomized double-blind, placebo-controlled study, 66 patients received placebo or transglucosidase (300 or 900 mg/day) orally, for 12 weeks...
October 2017: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/29025929/whole-genome-sequence-of-bifidobacterium-longum-strain-indica-isolated-from-the-gut-of-a-healthy-indian-adult
#6
Satyabrata Bag, Tarini Shankar Ghosh, Bhabatosh Das
Bifidobacterium longum, a Gram-positive rod-shaped anaerobic bacterium, inhabits the human gastrointestinal tract and contributes significantly to oligosaccharide production, amino acid metabolism, and protection against intestinal inflammation. Here, we report the whole-genome sequence of B. longum, which was isolated from the gastrointestinal tract of a healthy Indian adult.
October 12, 2017: Genome Announcements
https://www.readbyqxmd.com/read/29025837/effects-of-lactobacillus-rhamnosus-gg-and-bifidobacterium-lactis-bb12-on-beta-cell-function-in-children-with-newly-diagnosed-type-1-diabetes-protocol-of-a-randomised-controlled-trial
#7
Lidia Groele, Hania Szajewska, Agnieszka Szypowska
INTRODUCTION: Recent evidence has demonstrated that, among other factors, dysbiosis (imbalances in the composition and function of the gut microbiota) may be relevant in the development of type 1 diabetes (T1D). Thus, gut microbiota may be a target for improving outcomes in subjects with T1D. The aim of the study is to examine the effects of Lactobacillus rhamnosus GG and Bifidobacterium lactis Bb12 on beta-cell function in children with newly diagnosed T1D. METHODS AND ANALYSIS: A total of 96 children aged 8 to 17 years with newly diagnosed T1D, confirmed by clinical history and the presence of at least one positive autoantibody, will be enrolled in a double-blind, randomised, placebo-controlled trial in which they will receive L...
October 11, 2017: BMJ Open
https://www.readbyqxmd.com/read/29022648/probiotics-for-experimental-obesity-prevention-focus-on-strain-dependence-and-viability-of-composition
#8
Nazarii Kobyliak, Tetyana Falalyeyeva, Tetyana Beregova, Mykola Spivak
INTRODUCTION: a comparative animal study of the efficacy of intermittent short-course administration of lyophilised single-, three-, and live multistrain probiotic on obesity. METHODS: We included 70 rats divided into seven groups (n = 10 in each). Rats of group I were intact. Newborn rats of groups II-VII were injected with monosodium glutamate (MSG) (4 mg/g). Rats of group II (MSG-obesity group) were untreated. The group III-V received lyophilised monoprobiotics B...
October 12, 2017: Endokrynologia Polska
https://www.readbyqxmd.com/read/29022389/the-composition-and-metabolism-of-faecal-microbiota-is-specifically-modulated-by-different-dietary-polysaccharides-and-mucin-an-isothermal-microcalorimetry-study
#9
K Adamberg, K Kolk, M Jaagura, R Vilu, S Adamberg
The metabolic activity of colon microbiota is specifically affected by fibres with various monomer compositions, degree of polymerisation and branching. The supply of a variety of dietary fibres assures the diversity of gut microbial communities considered important for the well-being of the host. The aim of this study was to compare the impact of different oligo- and polysaccharides (galacto- and fructooligosaccharides, resistant starch, levan, inulin, arabinogalactan, xylan, pectin and chitin), and a glycoprotein mucin on the growth and metabolism of faecal microbiota in vitro by using isothermal microcalorimetry (IMC)...
October 12, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/29022385/effect-of-antibiotic-probiotic-and-human-rotavirus-infection-on-colonisation-dynamics-of-defined-commensal-microbiota-in-a-gnotobiotic-pig-model
#10
H-C Huang, A N Vlasova, A Kumar, S Kandasamy, D D Fischer, L Deblais, F C Paim, S N Langel, M A Alhamo, A Rauf, L Shao, L J Saif, G Rajashekara
We developed a gnotobiotic (Gn) pig model colonised with defined commensal microbiota (DMF) to provide a simplified and controlled system to study the interactions between intestinal commensals, antibiotics (ciprofloxacin, CIP), probiotics (Escherichia coli Nissle 1917, EcN) and virulent human rotavirus (VirHRV). The DMF included seven gut commensal species of porcine origin that mimic the predominant species in the infant gut. Gn piglets were divided into four groups: DMF control (non-treated), DMF+CIP (CIP treated), DMF+CIP+EcN (CIP/EcN treated), DMF+EcN (EcN treated) and inoculated orally with 10(5) cfu of each DMF strain...
October 12, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/29022383/ratio-of-klebsiella-bifidobacterium-in-early-life-correlates-with-later-development-of-paediatric-allergy
#11
J S Y Low, S-E Soh, Y K Lee, K Y C Kwek, J D Holbrook, E M Van der Beek, L P Shek, A E N Goh, O H Teoh, K M Godfrey, Y-S Chong, J Knol, C Lay
Several studies have reported that intestinal microbial colonisation patterns differ between non-allergic and allergic infants. However, the microbial signature underlying the pathogenesis of allergies remains unclear. We aim to gain insight into the development of the intestinal microbiota of healthy infants and infants who develop allergy in early life, and identify potential microbiota biomarkers of later allergic disease. Using a case-control design in a Chinese sub-cohort of a Singaporean birth cohort (GUSTO), we utilised 16S rRNA gene sequencing to assess intestinal microbial composition and diversity of 21 allergic and 18 healthy infants at 3 weeks, 3 months and 6 months of age, and correlated the microbiota with allergy at ages 18 and 36 months...
October 12, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28994235/lipo-protein-emulsion-structure-in-the-diet-affects-protein-digestion-kinetics-intestinal-mucosa-parameters-and-microbiota-composition
#12
Marion Oberli, Véronique Douard, Martin Beaumont, Daphné Jaoui, Fabienne Devime, Sandy Laurent, Catherine Chaumontet, Damien Mat, Steven Le Feunteun, Camille Michon, Anne-Marie Davila, Gilles Fromentin, Daniel Tomé, Isabelle Souchon, Marion Leclerc, Claire Gaudichon, François Blachier
SCOPE: Food structure is a key factor controlling digestion and nutrient absorption. We tested the hypothesis that protein emulsion structure in the diet may affect digestive and absorptive processes. METHODS & RESULTS: Rats (n = 40) were fed for 3 weeks two diets chemically identical but based on lipid-protein liquid-fine (LFE) or gelled-coarse (GCE) emulsions that differ at the macro- and micro-structure levels. After an overnight fasting, they ingested a (15) N-labeled LFE or GCE test meal and were euthanized 0, 15min, 1h and 5h later...
October 10, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28987358/regulatory-efficacy-of-fermented-plant-extract-on-the-intestinal-microflora-and-lipid-profile-in-mildly-hypercholesterolemic-individuals
#13
Hui-Fang Chiu, Yen-Jung Chen, Yan-Ying Lu, Yi-Chun Han, You-Cheng Shen, Kamesh Venkatakrishnan, Chin-Kun Wang
In recent years, the use of fermented plant products to protect against various metabolic syndromes has been increasing enormously. The objective of this study was to check the regulatory efficacy of fermented plant extract (FPE) on intestinal microflora, lipid profile, and antioxidant status in mildly hypercholesterolemic volunteers. Forty-four mildly hypercholesterolemic individuals (cholesterol 180-220 mg/dL) were recruited and assigned to two groups: experimental or placebo. Volunteers were requested to drink either 60 mL of FPE or placebo for 8 weeks...
October 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28983778/probiotics-for-the-prevention-of-hirschsprung-associated-enterocolitis-a-systematic-review-and-meta-analysis
#14
H Nakamura, T Lim, P Puri
BACKGROUND/PURPOSE: Hirschsprung-associated enterocolitis (HAEC) is a life-threatening complication of Hirschsprung's disease. HAEC is reported to occur in 6-50% of patients preoperatively and in 2-35% postoperatively. The exact cause of HAEC is not fully understood, but disturbances of intestinal microbiota have recently been reported in patients with HAEC. In recent years, the administration of probiotics has been proposed to reduce the incidence of HAEC. We conducted a systematic review and meta-analysis to determine the effect of probiotics on postoperative HAEC...
October 5, 2017: Pediatric Surgery International
https://www.readbyqxmd.com/read/28983638/bifidobacteria-and-the-infant-gut-an-example-of-co-evolution-and-natural-selection
#15
REVIEW
Francesca Turroni, Christian Milani, Sabrina Duranti, Chiara Ferrario, Gabriele Andrea Lugli, Leonardo Mancabelli, Douwe van Sinderen, Marco Ventura
Throughout the human life, the gut microbiota interacts with us in a number of different ways, thereby influencing our health status. The acquisition of such an interactive gut microbiota commences at birth. Medical and environmental factors including diet, antibiotic exposure and mode of delivery are major factors that shape the composition of the microbial communities in the infant gut. Among the most abundant members of the infant microbiota are species belonging to the Bifidobacterium genus, which are believed to confer beneficial effects upon their host...
October 5, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28983003/complete-genome-sequence-of-a-strain-of-bifidobacterium-pseudolongum-isolated-from-mouse-feces-and-associated-with-improved-organ-transplant-outcome
#16
Emmanuel F Mongodin, Lauren L Hittle, Suvarna Nadendla, C Colin Brinkman, Yanbao Xiong, Jonathan S Bromberg
Here, we report the complete genome sequence of Bifidobacterium pseudolongum strain UMB-MBP-01, isolated from the feces of C57BL/6J mice. This strain was identified in microbiome profiling studies and associated with improved transplant outcome in a murine model of cardiac heterotypic transplantation.
October 5, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28979247/probiotic-mixture-golden-bifido-prevents-neonatal-escherichia-coli-k1-translocation-via-enhancing-intestinal-defense
#17
Qing Zeng, Xiaolong He, Santhosh Puthiyakunnon, Hansen Xiao, Zelong Gong, Swapna Boddu, Lecheng Chen, Huiwen Tian, Sheng-He Huang, Hong Cao
Escherichia coli (E. coli) K1 sepsis and meningitis is a severe infection characterized by high mortality in neonates. Successful colonization and translocation across the intestinal mucosa have been regarded as the critical steps for E. coli K1 sepsis and meningitis. We recently reported that the probiotic mixture, Golden Bifido (containing live Lactobacillus bulgaricus, Bifidobacterium, and Streptococcus thermophilus, LBS) has a preventive role against neonatal E. coli K1 bacteremia and meningitis. However, the interaction between the neonatal gut barrier, probiotics and E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28976925/prebiotics-mediate-microbial-interactions-in-a-consortium-of-the-infant-gut-microbiome
#18
Daniel A Medina, Francisco Pinto, Aline Ovalle, Pamela Thomson, Daniel Garrido
Composition of the gut microbiome is influenced by diet. Milk or formula oligosaccharides act as prebiotics, bioactives that promote the growth of beneficial gut microbes. The influence of prebiotics on microbial interactions is not well understood. Here we investigated the transformation of prebiotics by a consortium of four representative species of the infant gut microbiome, and how their interactions changed with dietary substrates. First, we optimized a culture medium resembling certain infant gut parameters...
October 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28975455/potential-prebiotic-oligosaccharide-mixtures-from-acidic-hydrolysis-of-rice-bran-and-cassava-pulp
#19
Chanida Hansawasdi, Peter Kurdi
Two agricultural wastes, rice bran and cassava pulp were subjected to acidic hydrolysis by 2 M sulfuric acid which resulted in hemicellulosic oligosaccharide mixtures. Monosaccharide component analysis of these mixtures revealed that the oligosaccharides of rice bran acid hydrolysate (RAHF) composed of glucose and arabinose while cassava pulp acid hydrolysate (CAHF) was found to be comprised of glucose, galactose and arabinose. Both RAHF and CAHF were able to fuel all of the tested three Lactobacillus, five Bifidobacterium and three Bacteroides strains indicating the prebiotic potential of these oligosaccharide mixtures...
October 3, 2017: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/28974612/bifidobacterium-bifidum-extracellular-sialidase-enhances-adhesion-to-the-mucosal-surface-and-supports-carbohydrate-assimilation
#20
Keita Nishiyama, Yuji Yamamoto, Makoto Sugiyama, Takashi Takaki, Tadasu Urashima, Satoru Fukiya, Atsushi Yokota, Nobuhiko Okada, Takao Mukai
Bifidobacterium is a natural inhabitant of the human gastrointestinal (GI) tract. We studied the role of the extracellular sialidase (SiaBb2, 835 amino acids [aa]) from Bifidobacterium bifidum ATCC 15696 in mucosal surface adhesion and carbohydrate catabolism. Human milk oligosaccharides (HMOs) or porcine mucin oligosaccharides as the sole carbon source enhanced B. bifidum growth. This was impaired in a B. bifidum ATCC 15696 strain harboring a mutation in the siabb2 gene. Mutant cells in early to late exponential growth phase also showed decreased adhesion to human epithelial cells and porcine mucin relative to the wild-type strain...
October 3, 2017: MBio
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