keyword
MENU ▼
Read by QxMD icon Read
search

Phage Cocktail

keyword
https://www.readbyqxmd.com/read/29330592/genomic-characterization-of-key-bacteriophages-to-formulate-the-potential-biocontrol-agent-to-combat-enteric-pathogenic-bacteria
#1
Krupa M Parmar, Nishant A Dafale, Hitesh Tikariha, Hemant J Purohit
Combating bacterial pathogens has become a global concern especially when the antibiotics and chemical agents are failing to control the spread due to its resistance. Bacteriophages act as a safe biocontrol agent by selectively lysing the bacterial pathogens without affecting the natural beneficial microflora. The present study describes the screening of prominent enteric pathogens NDK1, NDK2, NDK3, and NDK4 (Escherichia, Klebsiella, Enterobacter, and Serratia) mostly observed in domestic wastewater; against which KNP1, KNP2, KNP3, and KNP4 phages were isolated...
January 12, 2018: Archives of Microbiology
https://www.readbyqxmd.com/read/29300761/investigation-of-prevalence-of-free-shiga-toxin-producing-escherichia-coli-stec-specific-bacteriophages-and-its-correlation-with-stec-bacterial-hosts-in-a-produce-growing-area-in-salinas-california
#2
Yen-Te Liao, Irwin A Quintela, Kimberly Nguyen, Alexandra Salvador, Michael B Cooley, Vivian C H Wu
Shiga toxin-producing E. coli (STEC) causes approximately 265,000 illnesses and 3,600 hospitalizations annually and is highly associated with animal contamination due to the natural reservoir of ruminant gastrointestinal tracts. Free STEC-specific bacteriophages against STEC strains are also commonly isolated from fecal-contaminated environment. Previous studies have evaluated the correlation between the prevalence of STEC-specific bacteriophages and STEC strains to improve animal-associated environment. However, the similar information regarding free STEC-specific bacteriophages prevalence in produce growing area is lacking...
2018: PloS One
https://www.readbyqxmd.com/read/29284014/isolation-and-characterization-of-a-n4-like-lytic-bacteriophage-infecting-vibrio-splendidus-a-pathogen-of-fish-and-bivalves
#3
Pantelis Katharios, Panos G Kalatzis, Constantina Kokkari, Elena Sarropoulou, Mathias Middelboe
A novel virulent bacteriophage, vB_VspP_pVa5, infecting a strain of Vibrio splendidus was isolated from a sea-cage aquaculture farm in Greece, and characterized using microbiological methods and genomic analysis. Bacteriophage vB_VspP_pVa5 is a N4-like podovirus with an icosahedral head measuring 85 nm in length and a short non-contractile tail. The phage had a narrow host range infecting only the bacterial host, a latent period of 30 min and a burst size of 24 virions per infected bacterium. Its genome size was 78,145 bp and genomic analysis identified 107 densely-packed genes, 40 of which could be annotated...
2017: PloS One
https://www.readbyqxmd.com/read/29282504/bacteriophages-as-biological-control-agents-of-enteric-bacteria-contaminating-edible-oysters
#4
Tuan Son Le, Paul C Southgate, Wayne O'Connor, Sue Poole, D Ipek Kurtbӧke
Bacterial contamination on seafood resulting from unhygienic food-handling practices causes foodborne diseases and significant revenue losses. Moreover, control measures are complicated by a high prevalence of antibiotic-resistant bacteria. Alternative measures such as the phage therapy, therefore, is considered as an environmental and consumer-friendly biological control strategy for controlling such bacterial contamination. In this study, we determined the effectiveness of a bacteriophage cocktail in controlling E...
December 27, 2017: Current Microbiology
https://www.readbyqxmd.com/read/29234314/efficacy-and-safety-of-a-bovine-associated-staphylococcus-aureus-phage-cocktail-in-a-murine-model-of-mastitis
#5
Koen Breyne, Ryan W Honaker, Zachary Hobbs, Manuela Richter, Maciej Żaczek, Taylor Spangler, Jonas Steenbrugge, Rebecca Lu, Anika Kinkhabwala, Bruno Marchon, Evelyne Meyer, Lucia Mokres
Overuse of antibiotics is a major problem in the treatment of bovine mastitis, and antibiotic treatment is frequently non-curative, thus alternative treatments are necessary. The primary aim of this study was to evaluate the efficacy of a purified phage cocktail for treatment of bovine Staphylococcus aureus mastitis in a well-defined mouse model. Candidate phages were selected based on their in vitro performance and subsequently processed into an optimally composed phage cocktail. The highest scoring phages were further tested for efficacy and resistance suppression in broth and raw milk, with and without supplemental IgG...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29201902/in-vivo-studies-on-the-influence-of-bacteriophage-preparations-on-the-autoimmune-inflammatory-process
#6
Ryszard Międzybrodzki, Jan Borysowski, Marlena Kłak, Ewa Jończyk-Matysiak, Bożena Obmińska-Mrukowicz, Agnieszka Suszko-Pawłowska, Barbara Bubak, Beata Weber-Dąbrowska, Andrzej Górski
Phage preparations used for phage therapy may have not only direct antibacterial action but also immunomodulating effects mediated by phages themselves as well as by bacterial antigens. Therefore phage application in patients with immune disorders, and especially with autoimmune diseases, requires special attention. The aim of this study was to investigate the effect of phage lysates (staphylococcal phages A3/R, phi200, and MS-1 cocktail, enterococcal phage 15/P, Pseudomonas phage 119x, and E. coli T4 phage) as well as purified T4 phage on the course of murine collagen-induced arthritis (CIA), commonly used as an animal model of rheumatoid arthritis...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29168148/developing-a-bacteriophage-cocktail-for-biocontrol-of-potato-bacterial-wilt
#7
Cuihua Wei, Junli Liu, Alice Nyambura Maina, Francis B Mwaura, Junping Yu, Chenghui Yan, Ruofang Zhang, Hongping Wei
Bacterial wilt is a devastating disease of potato and can cause an 80% production loss. To control wilt using bacteriophage therapy, we isolated and characterized twelve lytic bacteriophages from different water sources in Kenya and China. Based on the lytic curves of the phages with the pathogen Ralstonia solanacearum, one optimal bacteriophage cocktail, P1, containing six phage isolations was formulated and used for studying wilt prevention and treatment efficiency in potato plants growing in pots. The preliminary tests showed that the phage cocktail was very effective in preventing potato bacterial wilt by injection of the phages into the plants or decontamination of sterilized soil spiked with R...
November 16, 2017: Virologica Sinica
https://www.readbyqxmd.com/read/29150707/a-guard-killer-phage-cocktail-effectively-lyses-the-host-and-inhibits-the-development-of-phage-resistant-strains-of-escherichia-coli
#8
Ling Yu, Shuang Wang, Zhimin Guo, Hongtao Liu, Diangang Sun, Guangmou Yan, Dongliang Hu, Chongtao Du, Xin Feng, Wenyu Han, Jingmin Gu, Changjiang Sun, Liancheng Lei
In recent years, after the emergence of a large number of multidrug-resistant bacteria, phages and phage-associated products for the prevention and control of bacterial disease have revealed prominent advantages as compared with antibiotics. However, bacteria are susceptible to becoming phage-resistant, thus severely limiting the application of phage therapy. In this study, Escherichia coli cells were incubated with lytic bacteriophages to obtain mutants that were resistant to the lytic phages. Then, bacteriophages against the phage-resistant variants were isolated and subsequently mixed with the original lytic phage to prepare a novel phage cocktail for bactericidal use...
November 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29099783/metagenomic-analysis-of-therapeutic-pyo-phage-cocktails-from-1997-to-2014
#9
Julia Villarroel, Mette Voldby Larsen, Mogens Kilstrup, Morten Nielsen
Phage therapy has regained interest in recent years due to the alarming spread of antibiotic resistance. Whilst phage cocktails are commonly sold in pharmacies in countries such as Georgia and Russia, this is not the case in western countries due to western regulatory agencies requiring a thorough characterization of the drug. Here, DNA sequencing of constituent biological entities constitutes a first step. The pyophage (PYO) cocktail is one of the main commercial products of the Georgian Eliava Institute of Bacteriophage, Microbiology and Virology and is used to cure skin infections...
November 3, 2017: Viruses
https://www.readbyqxmd.com/read/29077053/differential-effect-of-newly-isolated-phages-belonging-to-pb1-like-phikz-like-and-luz24-like-viruses-against-multi-drug-resistant-pseudomonas-aeruginosa-under-varying-growth-conditions
#10
Simone Latz, Alex Krüttgen, Helga Häfner, Eva Miriam Buhl, Klaus Ritter, Hans-Peter Horz
In this study, we characterize three phages (SL1 SL2, and SL4), isolated from hospital sewage with lytic activity against clinical isolates of multi-drug resistant Pseudomonas aeruginosa (MDR-PA). The host spectrum ranged from 41% to 54%, with all three phages together covering 79% of all tested clinical isolates. Genome analysis revealed that SL1 (65,849 bp, 91 open reading frames ORFs) belongs to PB1-like viruses, SL2 (279,696 bp, 354 ORFs) to phiKZ-like viruses and SL4 (44,194 bp, 65 ORFs) to LUZ24-like viruses...
October 27, 2017: Viruses
https://www.readbyqxmd.com/read/29018773/activity-of-bacteriophages-in-removing-biofilms-of-pseudomonas-aeruginosa-isolates-from-chronic-rhinosinusitis-patients
#11
Stephanie A Fong, Amanda Drilling, Sandra Morales, Marjolein E Cornet, Bradford A Woodworth, Wytske J Fokkens, Alkis J Psaltis, Sarah Vreugde, Peter-John Wormald
Introduction:Pseudomonas aeruginosa infections are prevalent amongst chronic rhinosinusitis (CRS) sufferers. Many P. aeruginosa strains form biofilms, leading to treatment failure. Lytic bacteriophages (phages) are viruses that infect, replicate within, and lyse bacteria, causing bacterial death. Aim: To assess the activity of a phage cocktail in eradicating biofilms of ex vivo P.aeruginosa isolates from CRS patients. Methods: P. aeruginosa isolates from CRS patients with and without cystic fibrosis (CF) across three continents were multi-locus sequence typed and tested for antibiotic resistance...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28974033/the-european-antibotabe-framework-program-and-its-update-development-of-innovative-botulinum-antibodies
#12
REVIEW
Christine Rasetti-Escargueil, Arnaud Avril, Sebastian Miethe, Christelle Mazuet, Yagmur Derman, Katja Selby, Philippe Thullier, Thibaut Pelat, Remi Urbain, Alexandre Fontayne, Hannu Korkeala, Dorothea Sesardic, Michael Hust, Michel R Popoff
The goal of the AntiBotABE Program was the development of recombinant antibodies that neutralize botulinum neurotoxins (BoNT) A, B and E. These serotypes are lethal and responsible for most human botulinum cases. To improve therapeutic efficacy, the heavy and light chains (HC and LC) of the three BoNT serotypes were targeted to achieve a synergistic effect (oligoclonal antibodies). For antibody isolation, macaques were immunized with the recombinant and non-toxic BoNT/A, B or E, HC or LC, followed by the generation of immune phage-display libraries...
October 2, 2017: Toxins
https://www.readbyqxmd.com/read/28970228/expression-of-a-peptidoglycan-hydrolase-from-lytic-bacteriophages-atu_ph02-and-atu_ph03-triggers-lysis-of-agrobacterium-tumefaciens
#13
Hedieh Attai, Jeanette Rimbey, George P Smith, Pamela J B Brown
In order to provide food security, innovative approaches to preventing plant disease are currently being explored. Here, we demonstrate that lytic bacteriophages and phage lysis proteins are effective at triggering lysis of the phytopathogen Agrobacterium tumefaciens Phages Atu_ph02 and Atu_ph03 were isolated from wastewater and induce lysis of C58-derived strains of A. tumefaciens Co-inoculation of A. tumefaciens with phage onto potato discs limits tumor formation. The genomes of Atu_ph02 and Atu_ph03 are nearly identical and ∼42% identical to T7 supercluster phages...
September 29, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28964986/phage-therapy-as-an-alternative-or-complementary-strategy-to-prevent-and-control-biofilm-related-infections
#14
REVIEW
D P Pires, Ldr Melo, D Vilas Boas, S Sillankorva, J Azeredo
The complex heterogeneous structure of biofilms confers to bacteria an important survival strategy. Biofilms are frequently involved in many chronic infections in consequence of their low susceptibility to antibiotics as well as resistance to host defences. The increasing need of novel and effective treatments to target these complex structures has led to a growing interest on bacteriophages (phages) as a strategy for biofilm control and prevention. Phages can be used alone, as a cocktail to broaden the spectra of activity, or in combination with other antimicrobials to improve their efficacy...
September 28, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28917651/bacteriophages-as-an-alternative-to-conventional-antibiotic-use-for-the-prevention-or-treatment-of-paenibacillus-larvae-in-honeybee-hives
#15
T Scott Brady, Bryan D Merrill, Jared A Hilton, Ashley M Payne, Michael B Stephenson, Sandra Hope
American Foulbrood (AFB) is an infectious disease caused by the bacteria, Paenibacillus larvae. P. larvae phages were isolated and tested to determine each phages' host range amongst 59 field isolate strains of P. larvae. Three phages were selected to create a phage cocktail for the treatment of AFB infections according to the combined phages' ability to lyse all tested strains of bacteria. Studies were performed to demonstrate the safety and efficacy of the phage cocktail treatment as a replacement for traditional antibiotics for the prevention of AFB and the treatment of active infections...
September 14, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28914348/immunogenicity-and-antimicrobial-effectiveness-of-pseudomonas-aeruginosa-specific-bacteriophage-in-a-human-lung-in-vitro-model
#16
Joseph R Shiley, Kristen K Comfort, Jayne B Robinson
The rise of antibiotic resistant bacteria is posing a serious threat to human health. For example, resistant strains of Pseudomonas aeruginosa have resulted in untreatable and potentially lethal infections in both cystic fibrosis and immunocompromised patients. Due to the growing need for alternative treatment options, bacteriophage, or phage, therapy is gaining considerable attention. While previous studies have demonstrated the effectiveness of phage in combating persistent bacterial infections, there is currently a lack of knowledge regarding the host immunological response following phage exposure...
November 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28906479/characterization-of-vb_saum-frusau02-a-twort-like-bacteriophage-isolated-from-a-therapeutic-phage-cocktail
#17
Katarzyna Leskinen, Henni Tuomala, Anu Wicklund, Jenni Horsma-Heikkinen, Pentti Kuusela, Mikael Skurnik, Saija Kiljunen
Staphylococcus aureus is a commensal and pathogenic bacterium that causes infections in humans and animals. It is a major cause of nosocomial infections worldwide. Due to increasing prevalence of multidrug resistance, alternative methods to eradicate the pathogen are necessary. In this respect, polyvalent staphylococcal myoviruses have been demonstrated to be excellent candidates for phage therapy. Here we present the characterization of the bacteriophage vB_SauM-fRuSau02 (fRuSau02) that was isolated from a commercial Staphylococcus bacteriophage cocktail produced by Microgen (Moscow, Russia)...
September 14, 2017: Viruses
https://www.readbyqxmd.com/read/28757045/efficacy-of-potential-phage-cocktails-against-vibrio-harveyi-and-closely-related-vibrio-species-isolated-from-shrimp-aquaculture-environment-in-the-south-east-coast-of-india
#18
Nattan Stalin, Pappu Srinivasan
A diverse set of novel phages infecting the marine pathogenic Vibrio harveyi was isolated from shrimp aquaculture environments in the south east coast of India. Based on initial screening, three phages with a broad host range revealed that the growth inhibition of phage is relatively specific to V. harveyi. They were also able to infect V. alginolyticus and V. parahemolyticus that belonged to the Harveyi clade species from shrimp pond and sea coast environment samples. However, the impact of these phages on their host bacterium are well understood; a one-step growth curve experiment and transmission electron microscope (TEM) revealed three phages grouped under the Myoviridae (VHM1 and VHM2); Siphoviridae (VHS1) family...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28742812/broad-range-lytic-bacteriophages-that-kill-staphylococcus-aureus-local-field-strains
#19
Virginia Abatángelo, Natalia Peressutti Bacci, Carina A Boncompain, Ariel F Amadio, Soledad Carrasco, Cristian A Suárez, Héctor R Morbidoni
Staphylococcus aureus is a very successful opportunistic pathogen capable of causing a variety of diseases ranging from mild skin infections to life-threatening sepsis, meningitis and pneumonia. Its ability to display numerous virulence mechanisms matches its skill to display resistance to several antibiotics, including β-lactams, underscoring the fact that new anti-S. aureus drugs are urgently required. In this scenario, the utilization of lytic bacteriophages that kill bacteria in a genus -or even species- specific way, has become an attractive field of study...
2017: PloS One
https://www.readbyqxmd.com/read/28739792/transfersomal-phage-cocktail-is-an-effective-treatment-against-methicillin-resistant-staphylococcus-aureus-mediated-skin-and-soft-tissue-infections
#20
Sanjay Chhibber, Ashu Shukla, Sandeep Kaur
The emergence of drug resistance has rekindled interest in phage therapy as an alternative treatment option; its potency, safety, and proven efficacy are worth noting. However, phage therapy still suffers from issues of poor stability, narrow spectra, and poor pharmacokinetic profiles. Therefore, it is essential to look into the use of drug delivery systems for efficient delivery of lytic phages in vivo The present study evaluated the use of nanostructured lipid-based carriers, i.e., transfersomes, as transdermal delivery systems for encapsulating a methicillin-resistant Staphylococcus aureus (MRSA) phage cocktail...
October 2017: Antimicrobial Agents and Chemotherapy
keyword
keyword
117549
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"