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platelet releasate

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https://www.readbyqxmd.com/read/28928125/ck2%C3%AE-critically-regulates-thrombopoiesis-and-ca-2-triggered-platelet-activation-in-arterial-thrombosis-in-vivo
#1
Patrick Münzer, Britta Walker-Allgaier, Sascha Geue, Friederike Langhauser, Eva Geuss, David Stegner, Katja Aurbach, Daniela Semeniak, Madhumita Chatterjee, Irene Gonzalez Menendez, Melanie Märklin, Leticia Quintanilla-Martinez, Helmut R Salih, David W Litchfield, Thierry Buchou, Christoph Kleinschnitz, Florian Lang, Bernhard Nieswandt, Irina Pleines, Harald Schulze, Meinrad Gawaz, Oliver Borst
Platelets, anucleated megakaryocyte-derived cells, play a major role in hemostasis and arterial thrombosis. While protein kinase Casein Kinase 2 (CK2) is readily detected in megakaryocytes and platelets, the impact of CK2-dependent signaling on megakaryocyte/platelet (patho-) physiology has remained elusive. The present study explored the impact of the CK2 regulatory β-subunit on platelet biogenesis and activation. Megakaryocyte/platelet-specific genetic deletion of CK2β (ck2β(-/-) ) in mice resulted in a significant macrothrombocytopenia and an increased extramedullar megakaryopoiesis with enhanced proportion of premature platelets...
September 19, 2017: Blood
https://www.readbyqxmd.com/read/28925872/abdominal-aorta-aneurysm-aaa-is-there-a-role-for-prevention-and-therapy-using-antioxidants
#2
Joël Pincemail, Jean-Olivier Defraigne, Audrey Courtois, Adelin Albert, Jean-Paul Cheramy-Bien, Natzi Sakalihasan
Abdominal aortic aneurysm (AAA) is a degenerative disease that cause mortality in people aged > 65 years. Increased reactive oxygen species (ROS) and oxidative stress seems to play a pivotal role in AAA pathogenesis. Several sources of ROS have been identified in aortic tissues using experimental models: inflammation, increased activity of NAD(P)H or NOX, over-expression of inducible nitric oxide synthase (iNOS), uncoupled endothelial nitric oxide synthase (eNOS), platelets activation and iron release from hemoglobin...
September 18, 2017: Current Drug Targets
https://www.readbyqxmd.com/read/28922541/effects-of-collagen-peptides-intake-on-skin-ageing-and-platelet-release-in-chronologically-aged-mice-revealed-by-cytokine-array-analysis
#3
Hongdong Song, Ling Zhang, Yongkang Luo, Siqi Zhang, Bo Li
Action mechanisms underlying various biological activities of collagen peptides (CPs) remained to be elucidated. Cytokines may play an important role in mediating these health benefits of CPs. This study aimed to systemically examine the cytokines in skin and blood regulated by CPs intake. Thirteen-month-old female Kunming mice were administered with CPs for 2 months (0 or 400 mg/kg bodyweight/day). The cytokines in skin and plasma were analysed using a 53-cytokine array and corresponding ELISA kits. In skin, CPs intake significantly down-regulated placenta growth factor (PIGF-2), insulin-like growth factor (IGF)-binding protein (IGFBP) -2 and IGFBP-3, and up-regulated platelet factor 4 (PF4), serpin E1 and transforming growth factor (TGF)-β1 ...
September 18, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28921447/structure-pharmacology-and-roles-in-physiology-of-the-p2y12-receptor
#4
Ivar von Kügelgen
P2Y receptors are G-protein-coupled receptors (GPCRs) for extracellular nucleotides. The platelet ADP-receptor which has been denominated P2Y12 receptor is an important target in pharmacotherapy. The receptor couples to Gαi2 mediating an inhibition of cyclic AMP accumulation and additional downstream events including the activation of phosphatidylinositol-3-kinase and Rap1b proteins. The nucleoside analogue ticagrelor and active metabolites of the thienopyridine compounds ticlopidine, clopidogrel and prasugrel block P2Y12 receptors and, thereby, inhibit ADP-induced platelet aggregation...
September 12, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28917273/mechanisms-and-risk-factors-of-thrombosis-in-cancer
#5
REVIEW
Anna Falanga, Laura Russo, Viola Milesi, Alfonso Vignoli
The close relationship between cancer and thrombosis is known since more than a century. Venous thromboembolism (VTE) may be the first manifestation of an occult malignancy in an otherwise healthy individual. Cancer patients commonly present with abnormalities of laboratory coagulation tests, indicating an ongoing subclinical hypercoagulable condition. The results of laboratory tests demonstrate that a process of fibrin formation and removal parallels the development of malignancy, which is of particular interest since fibrin and other clotting products are important for both thrombogenesis and tumor progression...
October 2017: Critical Reviews in Oncology/hematology
https://www.readbyqxmd.com/read/28912956/cardiometabolic-effects-of-a-novel-sirt1-activator-srt2104-in-people-with-type-2-diabetes-mellitus
#6
Radzi M Noh, Sowmya Venkatasubramanian, Shruti Daga, Jeremy Langrish, Nicholas L Mills, Ninian N Lang, Ethan Hoffmann, Brian Waterhouse, David E Newby, Brian M Frier
BACKGROUND: The cardiometabolic effects of SRT2104, a novel SIRT1 activator, were investigated in people with type 2 diabetes mellitus (T2DM). METHODS: Fifteen adults with T2DM underwent a randomised, double-blind, placebo-controlled cross-over trial and received 28 days of oral SRT2104 (2.0 g/day) or placebo. Forearm vasodilatation (measured during intrabrachial bradykinin, acetylcholine and sodium nitroprusside infusions) as well as markers of glycaemic control, lipid profile, plasma fibrinolytic factors, and markers of platelet-monocyte activation, were measured at baseline and at the end of each treatment period...
2017: Open Heart
https://www.readbyqxmd.com/read/28906481/insights-into-the-diagnostic-potential-of-extracellular-vesicles-and-their-mirna-signature-from-liquid-biopsy-as-early-biomarkers-of-diabetic-micro-macrovascular-complications
#7
REVIEW
Valeria La Marca, Alessandra Fierabracci
Extracellular vesicles (EVs) represent a heterogeneous population of small vesicles, consisting of a phospholipidic bilayer surrounding a soluble interior cargo. Almost all cell types release EVs, thus they are naturally present in all body fluids. Among the several potential applications, EVs could be used as drug delivery vehicles in disease treatment, in immune therapy because of their immunomodulatory properties and in regenerative medicine. In addition to general markers, EVs are characterized by the presence of specific biomarkers (proteins and miRNAs) that allow the identification of their cell or tissue origin...
September 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28906046/the-unique-n-terminal-zinc-finger-of-synaptotagmin-like-protein-4-reveals-fyve-structure
#8
Kazuhide Miyamoto, Arisa Nakatani, Kazuki Saito
Synaptotagmin-like protein 4 (Slp4), expressed in human platelets, is associated with dense granule release. Slp4 is comprised of the N-terminal zinc finger, Slp homology domain, and C2 domains. We synthesized a compact construct (the Slp4N peptide) corresponding to the Slp4 N-terminal zinc finger. Herein, we have determined the solution structure of the Slp4N peptide by NMR. Furthermore, experimental, chemical modification of Cys residues revealed that the Slp4N peptide binds two zinc atoms to mediate proper folding...
September 14, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28905392/cryopreserved-platelets-demonstrate-reduced-activation-responses-and-impaired-signaling-after-agonist-stimulation
#9
Lauren Waters, Matthew P Padula, Denese C Marks, Lacey Johnson
BACKGROUND: Room temperature-stored (20-24°C) platelets (PLTs) have a shelf life of 5 days, making it logistically challenging to supply remote medical centers with PLT products. Cryopreservation of PLTs in dimethyl sulfoxide (DMSO) and storage at -80°C enables an extended shelf life up to 2 years. Although cryopreserved PLTs have been widely characterized under resting conditions, their ability to undergo agonist-induced activation is yet to be fully explored. STUDY DESIGN AND METHODS: Buffy coat PLTs were cryopreserved at -80°C with 5% to 6% DMSO and sampled before freezing and after thawing...
September 14, 2017: Transfusion
https://www.readbyqxmd.com/read/28903944/a-dual-role-for-the-class-iii-pi3k-vps34-in-platelet-production-and-thrombus-growth
#10
Colin Valet, Marie Levade, Gaëtan Chicanne, Benoit Bilanges, Cendrine Cabou, Julien Viaud, Marie-Pierre Gratacap, Frédérique Gaits-Iacovoni, Bart Vanhaesebroeck, Bernard Payrastre, Sonia Severin
To uncover the role of Vps34, the sole class III phosphoinositide 3-kinase, in megakaryocytes (MKs) and platelets, we created a mouse model with Vps34 deletion in the MK/platelet lineage (Pf4-Cre/Vps34(lox/lox)). Deletion of Vps34 in MKs led to the loss of its regulator protein Vps15, and was associated with microthrombocytopenia and platelet granule abnormalities. While Vps34 deficiency did not impact on MK polyploidisation or proplatelet formation, it dampened MK granule biogenesis and directional migration towards an SDF1α gradient, leading to ectopic platelet release within the bone marrow...
September 13, 2017: Blood
https://www.readbyqxmd.com/read/28899579/cxcl4-induced-macrophages-in-human-atherosclerosis
#11
Gabriele Domschke, Christian A Gleissner
Atherosclerosis is considered an inflammatory disease of the arterial wall. Monocytes and monocyte-derived cells (most often termed macrophages) play an essential role in the formation of atherosclerotic lesions, as they take up lipids leading to subsequent foam cell formation accompanied by release of pro-inflammatory cytokines. Similarly, platelets have been discovered to represent an important cell type mediating inflammatory and immune processes in atherogenesis, mainly by secreting chemokines, which are stored in the platelets' alpha granules, upon platelet activation...
September 9, 2017: Cytokine
https://www.readbyqxmd.com/read/28899404/extracellular-vesicle-packaged-mirna-release-after-short-term-exposure-to-particulate-matter-is-associated-with-increased-coagulation
#12
Laura Pergoli, Laura Cantone, Chiara Favero, Laura Angelici, Simona Iodice, Eva Pinatel, Mirjam Hoxha, Laura Dioni, Marilena Letizia, Benedetta Albetti, Letizia Tarantini, Federica Rota, Pier Alberto Bertazzi, Amedea Silvia Tirelli, Vincenza Dolo, Andrea Cattaneo, Luisella Vigna, Cristina Battaglia, Michele Carugno, Matteo Bonzini, Angela Cecilia Pesatori, Valentina Bollati
BACKGROUND: Exposure to particulate matter (PM) is associated with increased incidence of cardiovascular disease and increased coagulation, but the molecular mechanisms underlying these associations remain unknown. Obesity may increase susceptibility to the adverse effects of PM exposure, exacerbating the effects on cardiovascular diseases. Extracellular vesicles (EVs), which travel in body fluids and transfer microRNAs (miRNAs) between tissues, might play an important role in PM-induced cardiovascular risk...
August 24, 2017: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/28895869/amiloride-interferes-with-platelet-activating-factor-induced-respiratory-burst-and-mmp-9-release-in-bovine-neutrophils-independent-of-na-h-exchanger-1
#13
C S Larrazabal, M D Carretta, M A Hidalgo, R A Burgos
Cytoplasmic pH homeostasis is required for an appropriate response in polymorphonuclear neutrophils (PMNs). In these cells, chemotaxis and reactive oxygen species (ROS) production are reduced by the use of Na(+)/H(+) exchanger (NHE-1) inhibitors, but these results are mainly obtained using amiloride, a non-selective NHE-1 inhibitor. In bovine PMNs, the role of NHE-1 in functional responses has not been confirmed yet. The aim of this study was to determine the role of NHE-1 using amiloride and zoniporide in pH regulation, ROS production, matrix metalloproteinase 9 (MMP-9) release and calcium flux in bovine PMNs induced by the platelet activation factor (PAF), additionally we evaluated the presence of NHE-1 and NHE-2 mRNA Our data show the presence only of NHE-1 but not NHE-2 in bovine PMNs...
September 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28890257/effects-of-pdgf-bb-delivery-from-heparinized-collagen-sutures-on-the-healing-of-lacerated-chicken-flexor-tendon-in-vivo
#14
Mousa Younesi, Derrick M Knapik, Jameson Cumsky, Baris Ozgur Donmez, Ping He, Anowarul Islam, Greg Learn, Philip McClellan, Michael Bohl, Robert J Gillespie, Ozan Akkus
Flexor tendon lacerations are traditionally repaired by using non-absorbable monofilament sutures. Recent investigations have explored to improve the healing process by growth factor delivery from the sutures. However, it is difficult to conjugate growth factors to nylon or other synthetic sutures. This study explores the performance of a novel electrochemically aligned collagen suture in a flexor tendon repair model with and without platelet derived growth factor following complete tendon laceration in vivo...
September 7, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28887865/plasma-rich-plasma-the-ultimate-secret-for-youthful-skin-elixir-and-hair-growth-triggering
#15
REVIEW
Ebtisam Elghblawi
The clinical application of platelet-rich plasma (PRP) is based on the increase in the concentration of growth factors that are released from alpha-granule of the concentrated platelets and in the secretion of proteins which are able to capitalize on the healing process at the cellular level. It has been invented to restore the natural beauty by starting the natural rejuvenation process of the skin and aiming to make it function as a younger one and to keep the skin youthful and maintain it. Besides that, it is also emerged to include hairs as a new injectable procedure to enable stimulating hair growth locally and topically; preventing its fall; improving hair shaft, hair stem, and its caliber; increasing its shine, vitality, and pliability; and declining hair splitting and breakage...
September 8, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28881440/injectable-mussel-inspired-immobilization-of-platelet-rich-plasma-on-microspheres-bridging-adipose-micro-tissues-to-improve-autologous-fat-transplantation-by-controlling-release-of-pdgf-and-vegf-angiogenesis-stem-cell-migration
#16
Shaolong Zhou, Qiang Chang, Feng Lu, Malcolm Xing
Platelets-rich plasma (PRP) can produce growth factors (GFs) to improve angiogenesis. However, direct injection of PRP does not lead to highly localized GFs. The current study employs a mussel-inspired polydopamine to immobilize PRP on gelatin microspheres (GMs) with the purpose of bridging adipose micro-tissues to help implanted fat survive (GM-pDA-PRP). Enhanced PRP adhesion leads to a prolonged and localized production of GFs, which is verified by platelet counting and by ELISA of vascular endothelial growth factors (VEGFs) and of platelet derived growth factors (PDGFs)...
September 7, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28880464/dual-growth-factor-immobilized-asymmetrically-porous-membrane-for-bone-to-tendon-interface-regeneration-on-rat-patellar-tendon-avulsion-model
#17
Joong-Hyun Kim, Se Heang Oh, Hyun Ki Min, Jin Ho Lee
Insufficient repair of the bone-to-tendon interface (BTI) with structural/compositional gradients has been a significant challenge in orthopedics. In this study, dual growth factor [platelet-derived growth factor-BB (PDGF-BB) and bone morphogenetic protein-2 (BMP-2)]-immobilized polycaprolactone (PCL)/Pluronic F127 asymmetrically porous membrane was fabricated to estimate its feasibility as a potential strategy for effective regeneration of BTI injury. The growth factors immobilized (via heparin-intermediated interactions) on the membrane were continuously released for up to ∼80% of the initial loading amount after 5 weeks without a significant initial burst...
September 7, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28880378/leukocyte-and-plasma-activation-profiles-in-chronically-transfused-patients-with-a-history-of-allergic-reactions
#18
Magali J Fontaine, Hank Shih, Richard Schubert, Wendy Wong, Jennifer Andrews, Michael Jeng, Rabindra Tirouvanziam
BACKGROUND: Allergic transfusion reactions are drawbacks to the benefits of transfusion. Classically, allergic transfusion reactions depend on histamine release from mast cells or basophils, but other leukocyte subsets may also be important. Thus, we propose to better define the exact leukocyte subsets involved in allergic transfusion reactions. STUDY DESIGN AND METHODS: The overall objective of the current study was to compare the activation of specific peripheral blood leukocyte subsets (monocytes, neutrophils, eosinophils, and basophils) in a cohort of 13 patients who received chronic transfusions and had a history of allergic transfusion reactions compared with a control group of patients who received chronic transfusions and had no history of allergic transfusion reactions...
September 6, 2017: Transfusion
https://www.readbyqxmd.com/read/28879551/clinical-grade-manufacturing-of-genetically-modified-car-expressing-nk-92-cells-for-the-treatment-of-erbb2-positive-malignancies
#19
Paulina Nowakowska, Annette Romanski, Nicole Miller, Marcus Odendahl, Halvard Bonig, Congcong Zhang, Erhard Seifried, Winfried S Wels, Torsten Tonn
BACKGROUND: The NK-92/5.28.z cell line (also referred to as HER2.taNK) represents a stable, lentiviral-transduced clone of ErbB2 (HER2)-specific, second-generation CAR-expressing derivative of clinically applicable NK-92 cells. This study addresses manufacturing-related issues and aimed to develop a GMP-compliant protocol for the generation of NK-92/5.28.z therapeutic doses starting from a well-characterized GMP-compliant master cell bank. MATERIALS AND METHODS: Commercially available GMP-grade culture media and supplements (fresh frozen plasma, platelet lysate) were evaluated for their ability to support expansion of NK-92/5...
September 6, 2017: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/28878758/differences-in-growth-properties-among-two-human-cytomegalovirus-glycoprotein-o-genotypes
#20
Julia Kalser, Barbara Adler, Michael Mach, Barbara Kropff, Elisabeth Puchhammer-Stöckl, Irene Görzer
Glycoprotein O (gO) of the human cytomegalovirus (HCMV) is the critical subunit of the envelope trimer gH/gL/gO as it interacts with platelet-derived growth factor alpha receptor upon fibroblast entry, and triggers gB-mediated fusion for fibroblast and epithelial cell infection. Eight genotypes (GT) of the highly polymorphic gO gene are described, yet it is unclear whether the distinct GTs differ in their function. Thus, we aimed to elucidate potential functional differences between two highly diverse gO GTs in an otherwise genomically identical HCMV strain...
2017: Frontiers in Microbiology
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