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https://www.readbyqxmd.com/read/28534411/intracellular-and-extracellular-recording-of-spontaneous-action-potentials-in-mammalian-neurons-and-cardiac-cells-with-3d-plasmonic-nanoelectrodes
#1
Michele Dipalo, Hayder Amin, Laura Lovato, Fabio Moia, Valeria Caprettini, Gabriele C Messina, Francesco Tantussi, Luca Berdondini, Francesco De Angelis
Three-dimensional vertical micro- and nanostructures can enhance the signal quality of multielectrode arrays and promise to become the prime methodology for the investigation of large networks of electrogenic cells. So far, access to the intracellular environment has been obtained via spontaneous poration, electroporation, or by surface functionalization of the micro/nanostructures; however, these methods still suffer from some limitations due to their intrinsic characteristics that limit their widespread use...
May 24, 2017: Nano Letters
https://www.readbyqxmd.com/read/28528237/skin-deformation-during-shoulder-movements-and-upper-extremity-activities
#2
Weiqing Ge, Alison Sfara, Brittany Hians
BACKGROUND: The necessity of sternal precautions for patients following cardiac surgery with median sternotomy has been questioned by clinicians, researchers, and even patients. The primary purpose was to determine if sternal skin deformation during shoulder movements and upper extremity activities is compressive or distractive and if there are any significant differences between the skin deformation at different positions during shoulder movements and upper extremity activities. The secondary purpose was to determine if sternal skin deformation is correlated with scapular stabilizer muscle strength...
May 12, 2017: Clinical Biomechanics
https://www.readbyqxmd.com/read/28520540/virtual-surgery-for-conduit-reconstruction-of-the-right-ventricular-outflow-tract
#3
Chin Siang Ong, Yue-Hin Loke, Justin Opfermann, Laura Olivieri, Luca Vricella, Axel Krieger, Narutoshi Hibino
PURPOSE: Virtual surgery involves the planning and simulation of surgical reconstruction using three-dimensional (3D) modeling based upon individual patient data, augmented by simulation of planned surgical alterations including implantation of devices or grafts. Here we describe a case in which virtual cardiac surgery aided us in determining the optimal conduit size to use for the reconstruction of the right ventricular outflow tract. DESCRIPTION: The patient is a young adolescent male with a history of tetralogy of Fallot with pulmonary atresia, requiring right ventricle-to-pulmonary artery (RV-PA) conduit replacement...
May 2017: World Journal for Pediatric & Congenital Heart Surgery
https://www.readbyqxmd.com/read/28512702/an-intracardiac-soft-robotic-device-for-augmentation-of-blood-ejection-from-the-failing-right-ventricle
#4
Markus A Horvath, Isaac Wamala, Eric Rytkin, Elizabeth Doyle, Christopher J Payne, Thomas Thalhofer, Ignacio Berra, Anna Solovyeva, Mossab Saeed, Sara Hendren, Ellen T Roche, Pedro J Del Nido, Conor J Walsh, Nikolay V Vasilyev
We introduce an implantable intracardiac soft robotic right ventricular ejection device (RVED) for dynamic approximation of the right ventricular (RV) free wall and the interventricular septum (IVS) in synchrony with the cardiac cycle to augment blood ejection in right heart failure (RHF). The RVED is designed for safe and effective intracardiac operation and consists of an anchoring system deployed across the IVS, an RV free wall anchor, and a pneumatic artificial muscle linear actuator that spans the RV chamber between the two anchors...
May 16, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28512434/evaluation-of-a-rapid-anisotropic-model-for-ecg-simulation
#5
Simone Pezzuto, Peter Kal'avský, Mark Potse, Frits W Prinzen, Angelo Auricchio, Rolf Krause
State-of-the-art cardiac electrophysiology models that are able to deliver physiologically motivated activation maps and electrocardiograms (ECGs) can only be solved on high-performance computing architectures. This makes it nearly impossible to adopt such models in clinical practice. ECG imaging tools typically rely on simplified models, but these neglect the anisotropic electric conductivity of the tissue in the forward problem. Moreover, their results are often confined to the heart-torso interface. We propose a forward model that fully accounts for the anisotropic tissue conductivity and produces the standard 12-lead ECG in a few seconds...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28510468/diagnostic-efficacy-of-coronary-artery-three-dimensional-steady-state-free-precession-magnetic-resonance-angiography-in-comparison-with-invasive-coronary-angiography-for-detecting-coronary-artery-disease
#6
Ali Mohammadzadeh, Fariborz Faeghi, Nazanin Sahraee, Hamidreza Pouraliakbar, Reza Kiani, Vahid Mohammadzadeh, Pouya Entezari, Ali Borhani, Madjid Shakiba, Sakineh Kadivar, Maryam Mohammadzadeh
PURPOSE: To assess the diagnostic value of three-dimensional steady-state free precession magnetic resonance angiography (3D-SSFP MRA) for detecting coronary artery disease (CAD). MATERIALS AND METHODS: Patients suspected of CAD based on clinical evaluation, underwent invasive coronary angiography (CAG) and Cardiac MRA (CMRA). Collected data in favor of any CAD findings in CMRA were compared to CAG results as the standard diagnostic method in CAD detection. Analysis was performed on per-patient, per-vessel and per-segment bases...
May 2017: Archives of Iranian Medicine
https://www.readbyqxmd.com/read/28501911/analysis-of-circumflex-artery-anatomy-by-real-time-3d-transesophageal-echocardiography-compared-to-cardiac-computed-tomography
#7
Carmine Bevilacqua, Sarah Eibel, Borek Foldyna, Thomas Knoefler, Lukas Lehmkuhl, Matthias Gutberlet, Chirojit Mukherjee, Joerg Seeburger, Piroze Davierwala, Joerg Ender
Iatrogenic injury to the circumflex artery (Cx) due to its close proximity to the mitral annulus is a rare but dreadful complication that can occur during mitral valve repair. The aim of our study was to compare multiple measurements of the Cx datasets, obtained by real time three-dimensional transesophageal echocardiography (RT3D TEE) and corresponding measurements assessed in multi-planar three-dimensional images acquired by multidetector computed tomography (MDCT). Preoperative RT3D TEE and MDCT datasets of 25 patients who had previously undergone minimally invasive mitral valve surgery were retrospectively analyzed...
May 13, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28498955/transcatheter-transapical-left-ventricle-remodelling-in-ischaemic-cardiomyopathy-with-apical-aneurysm-using-3rd-generation-3d-cardiac-computed-tomography-fusion-imaging
#8
Ralph Stephan von Bardeleben, Walter Kasper-König, Efthemidis Sotiriou, Andres Beiras-Fernandez, Christian Friedrich Vahl, Thomas Münzel, Eberhard Schulz
No abstract text is available yet for this article.
May 11, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28498548/engineered-3d-cardiac-fibrotic-tissue-to-study-fibrotic-remodeling
#9
Amir Hossein Sadeghi, Su Ryon Shin, Janine C Deddens, Giuseppe Fratta, Serena Mandla, Iman K Yazdi, Gyan Prakash, Silvia Antona, Danilo Demarchi, Marc P Buijsrogge, Joost P G Sluijter, Jesper Hjortnaes, Ali Khademhosseini
Activation of cardiac fibroblasts into myofibroblasts is considered to play an essential role in cardiac remodeling and fibrosis. A limiting factor in studying this process is the spontaneous activation of cardiac fibroblasts when cultured on two-dimensional (2D) culture plates. In this study, a simplified three-dimensional (3D) hydrogel platform of contractile cardiac tissue, stimulated by transforming growth factor-β1 (TGF-β1), is presented to recapitulate a fibrogenic microenvironment. It is hypothesized that the quiescent state of cardiac fibroblasts can be maintained by mimicking the mechanical stiffness of native heart tissue...
May 12, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28497844/echocardiographic-evaluation-of-right-ventricular-systolic-function-the-traditional-and-innovative-approach
#10
Dorota Smolarek, Marcin Gruchała, Wojciech Sobiczewski
Estimation of right ventricular (RV) performance still remains technically challenging due to its anatomical and functional distinctiveness. The current guidelines for the echocardiographic quantification of RV function recommend using multiple indices to describe the RV in a thorough and comprehensive manner, such as RV index of myocardial performance, tricuspid annular plane systolic excursion, fractional area change, Doppler tissue imaging-derived tricuspid lateral annular systolic velocity (S'-wave), three-dimensional RV ejection fraction (3D RVEF), longitudinal strain (RVLS)/strain rate by speckle-tracking echocardiography (STE)...
May 12, 2017: Cardiology Journal
https://www.readbyqxmd.com/read/28497486/5d-whole-heart-sparse-mri
#11
Li Feng, Simone Coppo, Davide Piccini, Jerome Yerly, Ruth P Lim, Pier Giorgio Masci, Matthias Stuber, Daniel K Sodickson, Ricardo Otazo
PURPOSE: A 5D whole-heart sparse imaging framework is proposed for simultaneous assessment of myocardial function and high-resolution cardiac and respiratory motion-resolved whole-heart anatomy in a single continuous noncontrast MR scan. METHODS: A non-electrocardiograph (ECG)-triggered 3D golden-angle radial balanced steady-state free precession sequence was used for data acquisition. The acquired 3D k-space data were sorted into a 5D dataset containing separated cardiac and respiratory dimensions using a self-extracted respiratory motion signal and a recorded ECG signal...
May 11, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28496076/-early-results-of-complex-fractionated-atrial-electrogram-mapping-guided-atrial-fibrillation-surgery
#12
Kazuaki Fukahara, Mari Sakai, Shigeki Yokoyama, Toshio Doi, Naoki Yoshimura
OBJECTIVE: Although the Cox-maze operation is the standard surgical procedure for permanent atrial fibrillation(AF), conversion to sinus rhythm is limited by patient characteristics, including the duration of AF, atrial size, and voltage of fibrillation waves. A surgical strategy based on structural alteration of the electrical substrates of AF is required to achieve better outcomes of AF surgery. Complex fractionated atrial electrogram (CFAE) plays an important role in the electrical substrate of AF...
May 2017: Kyobu Geka. the Japanese Journal of Thoracic Surgery
https://www.readbyqxmd.com/read/28494717/gelatin-as-biomaterial-for-tissue-engineering
#13
Mari Carmen Echave, Laura Saenz Del Burgo, Jose Luis Pedraz, Gorka Orive
Tissue engineering is considered one of the most important therapeutic strategies of regenerative medicine. The main objective of these new technologies is the development of substitutes made with biomaterials that are able to heal, repair or regenerate injured or diseased tissues and organs. These constructs seek to unlock the limited ability of human tissues and organs to regenerate. In this review, we highlight the convenient intrinsic properties of gelatin for the design and development of advanced systems for tissue engineering...
May 11, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28493526/real-time-mri-guidance-of-cardiac-interventions
#14
Adrienne E Campbell-Washburn, Mohammad A Tavallaei, Mihaela Pop, Elena K Grant, Henry Chubb, Kawal Rhode, Graham A Wright
Cardiac magnetic resonance imaging (MRI) is appealing to guide complex cardiac procedures because it is ionizing radiation-free and offers flexible soft-tissue contrast. Interventional cardiac MR promises to improve existing procedures and enable new ones for complex arrhythmias, as well as congenital and structural heart disease. Guiding invasive procedures demands faster image acquisition, reconstruction and analysis, as well as intuitive intraprocedural display of imaging data. Standard cardiac MR techniques such as 3D anatomical imaging, cardiac function and flow, parameter mapping, and late-gadolinium enhancement can be used to gather valuable clinical data at various procedural stages...
May 11, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28492526/differentiation-of-cardiomyocytes-and-generation-of-human-engineered-heart-tissue
#15
Kaja Breckwoldt, David Letuffe-Brenière, Ingra Mannhardt, Thomas Schulze, Bärbel Ulmer, Tessa Werner, Anika Benzin, Birgit Klampe, Marina C Reinsch, Sandra Laufer, Aya Shibamiya, Maksymilian Prondzynski, Giulia Mearini, Dennis Schade, Sigrid Fuchs, Christiane Neuber, Elisabeth Krämer, Umber Saleem, Mirja L Schulze, Marita L Rodriguez, Thomas Eschenhagen, Arne Hansen
Since the advent of the generation of human induced pluripotent stem cells (hiPSCs), numerous protocols have been developed to differentiate hiPSCs into cardiomyocytes and then subsequently assess their ability to recapitulate the properties of adult human cardiomyocytes. However, hiPSC-derived cardiomyocytes (hiPSC-CMs) are often assessed in single-cell assays. A shortcoming of these assays is the limited ability to characterize the physiological parameters of cardiomyocytes, such as contractile force, due to random orientations...
June 2017: Nature Protocols
https://www.readbyqxmd.com/read/28488097/feasibility-of-measurements-of-valve-dimensions-in-en-face-3d-transesophageal-echocardiography
#16
Sarah Eibel, Edwin Turton, Chirojit Mukherjee, Carmine Bevilacqua, Joerg Ender
Newest 3D software allows measurements directly in the en-face-3D TEE mode. Aim of the study was to ascertain whether measurements performed in the en-face-3D TEE mode are comparable with conventional measurement methods based on 2D TEE and 3D using the multiple plane reconstruction mode with the Qlab(®) software. En-face-3D TEE is used more frequently in daily clinical routine during cardiac operations. So far measurements could only be done based on 2D images or with the use of multi planar reconstruction mode with additional software...
May 9, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28483597/sub-microscopic-analysis-of-t-tubule-geometry-in-living-cardiac-ventricular-myocytes-using-a-shape-based-analysis-method
#17
Cherrie H T Kong, Eva A Rog-Zielinska, Clive H Orchard, Peter Kohl, Mark B Cannell
Transverse-axial tubules (TTs) are key structures involved in cardiac excitation-contraction coupling and can become deranged in disease. Although optical measurement of TTs is frequently employed to assess TT abundance and regularity, TT dimensions are generally below the diffraction limit of optical microscopy so determination of tubule size is problematic. TT diameter was measured by labeling both local surface membrane area and volume with fluorescent probes (FM4-64 and calcein, respectively), correcting image asymmetry by image processing and using the relationship between surface area and volume for a geometric primitive...
May 5, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28482139/contributions-of-bmps-in-cardiac-repair-cells-in-3d-in-vitro-models-and-angiogenesis
#18
Isabella Pallotta, Bruce Sun, Gregory Lallos, Cecile Terrenoire, Donald O Freytes
One of the main efforts in myocardial tissue engineering consists of designing cardiac tissues able to rescue the reduction in heart function once implanted at the site of myocardial infarction. To date, the efficiency of this approach in pre-clinical applications is limited in part by our incomplete understanding of the inflammatory environment known to be present at the site of myocardial infarct and by poor vascularization. We recently reported that polarized macrophages known to be present at the site of myocardial infarction secrete BMP-2 and BMP-4 proteins causing changes in the expression of cardiac proteins in a 2D in vitro model...
May 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28481037/regional-quantification-of-myocardial-mechanics-in-rat-using-3d-cine-dense-cardiovascular-magnetic-resonance
#19
Xiaoyan Zhang, Zhan-Qiu Liu, Dara Singh, Gregory J Wehner, David K Powell, Kenneth S Campbell, Brandon K Fornwalt, Jonathan F Wenk
Rat models have assumed an increasingly important role in cardiac research. However, a detailed profile of regional cardiac mechanics, such as strains and torsion, is lacking for rats. We hypothesized that healthy rat left ventricles (LVs) exhibit regional differences in cardiac mechanics, which are part of normal function. In this study, images of the LV were obtained with 3D cine displacement encoding with stimulated echoes (DENSE) cardiovascular magnetic resonance in 10 healthy rats. To evaluate regional cardiac mechanics, the LV was divided into basal, mid-ventricular, and apical regions...
May 8, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28479938/statistical-shape-modeling-for-cavopulmonary-assist-device-development-variability-of-vascular-graft-geometry-and-implications-for-hemodynamics
#20
Jan L Bruse, Giuliano Giusti, Catriona Baker, Elena Cervi, Tain-Yen Hsia, Andrew M Taylor, Silvia Schievano
Patients born with a single functional ventricle typically undergo three-staged surgical palliation in the first years of life, with the last stage realizing a cross-like total cavopulmonary connection (TCPC) of superior and inferior vena cavas (SVC and IVC) with both left and right pulmonary arteries, allowing all deoxygenated blood to flow passively back to the lungs (Fontan circulation). Even though within the past decades more patients survive into adulthood, the connection comes at the prize of deficiencies such as chronic systemic venous hypertension and low cardiac output, which ultimately may lead to Fontan failure...
June 2017: Journal of Medical Devices
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