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https://www.readbyqxmd.com/read/29656218/improved-hypocrellin-a-production-in-shiraia-bambusicola-by-light-dark-shift
#1
Chun Xiao Sun, Yan Jun Ma, Jian Wen Wang
Hypocrellin A (HA) is a major bioactive perylenequinone from the fruiting body of Shiraia bambusicola used for the treatment of skin diseases and developed as a photodynamic therapy (PDT) agent against cancers and viruses. The mycelial culture of S. bambusicola under dark is a biotechnological alternative for HA production but with low yield. In this study, light and dark conditions were investigated to develop effective elicitation on HA production in the cultures. Our results showed the constant light at 200 lx stimulated HA production without any growth retardation of mycelia...
April 9, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29656178/generation-of-3-spinocerebellar-ataxia-type-1-sca1-patient-derived-induced-pluripotent-stem-cell-lines-lumci002-a-b-and-c-and-2-unaffected-sibling-control-induced-pluripotent-stem-cell-lines-lumci003-a-and-b
#2
Ronald A M Buijsen, Sarah L Gardiner, Marga J Bouma, Linda M van der Graaf, Merel W Boogaard, Barry A Pepers, Bert Eussen, Annelies de Klein, Christian Freund, Willeke M C van Roon-Mom
Spinocerebellar ataxia type 1 (SCA1) is a hereditary neurodegenerative disease caused by a CAG repeat expansion in exon 8 of the ATXN1 gene. We generated induced pluripotent stem cells (hiPSCs) from a SCA1 patient and his non-affected sister by using non-integrating Sendai Viruses (SeV). The resulting hiPSCs are SeVfree, express pluripotency markers, display a normal karyotype, retain the mutation (length of the CAG repeat expansion in the ATXN1 gene) and are able to differentiate into the three germ layers in vitro...
April 5, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29656087/molecular-features-of-hepatitis-e-virus-circulation-in-environmental-and-human-samples
#3
H Fenaux, M Chassaing, S Berger, H Jeulin, A Gentilhomme, M Bensenane, J P Bronowicki, C Gantzer, I Bertrand, E Schvoerer
BACKGROUND AND OBJECTIVES: Hepatitis E virus (HEV) is emerging but its circulation between humans and the environment remains misunderstood. HEV ORF2 gene encodes the capsid playing a key role in viral interactions with surfaces, ORF3 products are involved in the viral cycle. Our aim was to study the molecular characteristics of ORF2 and ORF3 which could favor HEV fitness in patients and the environment. STUDY DESIGN: Samples from 69 patients with hepatitis (blood/stools), 20 urban wastewaters, 20 effluents of a pig slaughterhouse, 22 farm pigs (stools), 20 wild boars (liver/stools) were collected in North-Eastern France...
April 5, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29656059/caffeic-acid-inhibits-hcv-replication-via-induction-of-ifn%C3%AE-antiviral-response-through-p62-mediated-keap1-nrf2-signaling-pathway
#4
Jian Shen, Guifeng Wang, Jianping Zuo
Hepatitis C virus (HCV) infection and its related liver disease have constituted a heavy burden worldwide. It had been reported that Drinking coffee could decrease mortality risk of HCV infected patients. Caffeic Acid (CA), the Coffee-related organic acid could inhibit HCV replication, however, the detailed mechanism of CA against HCV is unclear. In this study, we showed that CA could notably inhibit HCV replication. Mechanism study demonstrated that CA could induce HO-1 expression, which would trigger the IFNα antiviral response, and the antiviral effect of CA was attenuated when HO-1 activity was inhibited by SnPP (an HO-1 inhibitor)...
April 12, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29655893/comparative-sequence-and-structural-analysis-of-indian-orf-viruses-based-on-major-envelope-immune-dominant-protein-f1l-an-homologue-of-pox-viral-p35-h3-protein
#5
Revanaiah Yogisharadhya, Veerakyathappa Bhanuprakash, Amit Kumar, Mianak Mondal, Sathish Bhadravati Shivachandra
Orf virus (ORFV), a member of the genus Parapoxvirus in the family Poxviridae, is the cause of orf, a highly contagious zoonotic viral disease that affects mainly sheep and goats. In the present study, the sequence and phylogenetic analysis of Indian ORFV isolates (n = 15) from natural outbreaks in sheep and goats belonging to different geographical regions were analysed on the basis of F1L gene along with homology modelling of F1L protein. Multiple sequence alignments revealed highly conserved C-terminus and heterogeneity of N-terminus region of F1L among all orf viruses studied...
April 12, 2018: Gene
https://www.readbyqxmd.com/read/29655836/incidence-of-hepatocellular-carcinoma-in-patients-with-hcv-associated-cirrhosis-treated-with-direct-acting-antiviral-agents
#6
Vincenza Calvaruso, Giuseppe Cabibbo, Irene Cacciola, Salvatore Petta, Salvatore Madonia, Alessandro Bellia, Fabio Tinè, Marco Distefano, Anna Licata, Lydia Giannitrapani, Tullio Prestileo, Giovanni Mazzola, Maria Antonietta Di Rosolini, Licia Larocca, Gaetano Bertino, Antonio Digiacomo, Francesco Benanti, Luigi Guarneri, Alfonso Averna, Carmelo Iacobello, Antonio Magro, Ignazio Scalisi, Fabio Cartabellotta, Francesca Savalli, Marco Barbara, Antonio Davì, Maurizio Russello, Gaetano Scifo, Giovanni Squadrito, Calogero Cammà, Giovanni Raimondo, Antonio Craxì, Vito Di Marco
BACKGROUND & AIMS: Studies have produced conflicting results of the incidence of hepatocellular carcinoma (HCC) in patients with in hepatitis C virus (HCV)-associated cirrhosis treated with direct-acting antivirals (DAAs). Data from clinics are needed to accurately assess the occurrence rate of HCC in patients with cirrhosis in the real world. METHODS: We collected data from a large prospective study of 2249 consecutive patients (mean age, 65.4 years and 56...
April 12, 2018: Gastroenterology
https://www.readbyqxmd.com/read/29655366/hiv-1-protease-with-leucine-zipper-fused-at-n-terminus-exhibits-enhanced-linker-amino-acid-dependent-activity
#7
Fu-Hsien Yu, Chin-Tien Wang
BACKGROUND: HIV-1 protease (PR) activation is triggered by Gag-Pol dimerization. Premature PR activation results in reduced virion yields due to enhanced Gag cleavage. A p6* transframe peptide located directly upstream of protease is believed to play a modulating role in PR activation. Previous reports indicate that the C-terminal p6* tetra-peptide prevents premature PR activation triggered by a leucine zipper (LZ) dimerization motif inserted in the deleted p6* region. To clarify the involvement of C-terminal p6* residues in mitigating enhanced LZ-incurred Gag processing, we engineered constructs containing C-terminal p6* residue substitutions with and without a mutation blocking the p6*/PR cleavage site, and created other Gag or p6* domain-removing constructs...
April 14, 2018: Retrovirology
https://www.readbyqxmd.com/read/29653302/jembrana-disease-virus-vif-antagonizes-the-inhibition-of-bovine-apobec3-proteins-through-ubiquitin-mediate-protein-degradation
#8
Xing Su, Hong Wang, Xiaohong Zhou, Zhaolong Li, Baisong Zheng, Wenyan Zhang
Viral infectivity factor (Vif) encoded by lentiviruses is essential for viral replication and escaping from antiviral activity of host defensive factors APOBEC3. Jembrana disease virus (JDV) causes an acute disease syndrome with approximately 20% case fatality rate in Bali cattle. However, the interplay mechanism between JDV Vif and Bos taurus APOBEC3 (btA3) is poorly understood. In this study, we determined that JDV Vif recruits ElonginB, ElonginC(ELOB/C), Cul2 and RBX1 but without the need of CBF-β to form E3 ubiquitin ligase and induces the degradation of btA3 proteins...
April 10, 2018: Virology
https://www.readbyqxmd.com/read/29653132/sofosbuvir-inhibits-hepatitis-a-virus-replication-in-vitro-assessed-by-a-cell-based-fluorescent-reporter-system
#9
Wang Jiang, Fawad Muhammad, Pengjuan Ma, Xiyu Liu, Gang Long
Hepatitis A virus (HAV) infection remains a major cause of acute hepatitis worldwide and even leads to fulminant hepatitis. For screening antivirals against HAV in vitro, we develop a cell-based fluorescent reporter system named Huh-7.5.1-GA, in which HAV infection is visualized by green fluorescence protein (GFP) translocation from the cytosol into the nucleus. The reliability of Huh-7.5.1-GA for antiviral studies is validated by IFN-α, a known inhibitor of HAV replication, which impedes GFP translocation...
April 10, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29653131/bet-bromodomain-inhibitors-show-anti-papillomavirus-activity-in-vitro-and-block-crpv-wart-growth-in-vivo
#10
Mary A Morse, Karla K Balogh, Sarah A Brendle, Colin A Campbell, Mao X Chen, Rebecca C Furze, Isobel L Harada, Ian D Holyer, Umesh Kumar, Kevin Lee, Rab K Prinjha, Martin Rüdiger, Jon Seal, Simon Taylor, Jason Witherington, Neil D Christensen
The DNA papillomaviruses infect squamous epithelium and can cause persistent, benign and sometimes malignant hyperproliferative lesions. Effective antiviral drugs to treat human papillomavirus (HPV) infection are lacking and here we investigate the anti-papillomavirus activity of novel epigenetic targeting drugs, BET bromodomain inhibitors. Bromodomain and Extra-Terminal domain (BET) proteins are host proteins which regulate gene transcription, they bind acetylated lysine residues in histones and non-histone proteins via bromodomains, functioning as scaffold proteins in the formation of transcriptional complexes at gene regulatory regions...
April 10, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29652855/a-new-model-for-the-dynamics-of-hepatitis-c-infection-derivation-analysis-and-implications
#11
Philip J Aston
We review various existing models of hepatitis C virus (HCV) infection and show that there are inconsistencies between the models and known behaviour of the infection. A new model for HCV infection is proposed, based on various dynamical processes that occur during the infection that are described in the literature. This new model is analysed, and three steady state branches of solutions are found when there is no stem cell generation of hepatocytes. Unusually, the branch of infected solutions that connects the uninfected branch and the pure infection branch can be found analytically and always includes a limit point, subject to a few conditions on the parameters...
April 13, 2018: Viruses
https://www.readbyqxmd.com/read/29652789/terpenoids-from-the-soft-coral-sinularia-sp-collected-in-yongxing-island
#12
Guo-Fei Qin, Xu-Li Tang, Yan-Ting Sun, Xiang-Chao Luo, Jing Zhang, Leen van Ofwegen, Ping-Jyun Sung, Ping-Lin Li, Guo-Qiang Li
Three new sesquiterpenoids (sinuketal ( 1 ), sinulins A and B ( 2 and 3 )) and two new cembranoids (sinulins C and D ( 4 and 5 )), as well as eight known sesquiterpenoids ( 6–13 ) and eight known cembranoids ( 14–21 ), were isolated from the Xisha soft coral Sinularia sp. Their structures were elucidated by extensive spectroscopic analysis. Compound 1 possesses an unprecedented isopropyl-branched bicyclo [6.3.0] undecane carbon skeleton with unique endoperoxide moiety, and a plausible biosynthetic pathway of it was postulated...
April 13, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29652194/molecular-docking-revealed-the-binding-of-nucleotide-side-inhibitors-to-zika-viral-polymerase-solved-structures
#13
A A Elfiky, A M Ismail
A new Zika virus (ZIKV) outbreak started in 2015. According to the World Health Organization, 84 countries confirmed ZIKV infection. RNA-dependent RNA polymerase (RdRp) was an appealing target for drug designers during the last two decades. Through molecular docking, we screened 16 nucleotide/side inhibitors against ZIKV RdRp. While the mode of interaction with ZIKV is different from that in the hepatitis C virus (HCV), nucleotide/side inhibitors in this study (mostly anti-HCV) showed promising binding affinities (-6...
May 2018: SAR and QSAR in Environmental Research
https://www.readbyqxmd.com/read/29652096/rapid-reconstitution-of-cmv-specific-t-cells-after-stem-cell-transplantation
#14
Thomas Widmann, Urban Sester, Tina Schmidt, Barbara C Gärtner, Jörg Schubert, Michael Pfreundschuh, Martina Sester
OBJECTIVE: As reconstitution of virus-specific T-cells is critical to control CMV-viremia following stem-cell transplantation (SCT) we characterized the dynamics in CMV-specific T-cell reconstitution after SCT. METHODS: CMV-specific T-cells from 51 SCT-recipients were prospectively quantified and phenotypically characterised by intracellular cytokine-staining after specific stimulation and HLA class-I specific pentamers using flow-cytometry. RESULTS: CMV-specific CD4 T-cells reconstituted after a median of 2...
April 13, 2018: European Journal of Haematology
https://www.readbyqxmd.com/read/29651773/complete-genome-sequence-of-peanut-virus-c-a-putative-novel-ilarvirus
#15
Seungmo Lim, Yun-Woo Jang, Jin Woo Bae, Yeong-Hoon Lee, Bong Choon Lee, Youngnam Yoon
We determined the complete genome sequence of a putative novel ilarvirus, tentatively named "peanut virus C" (PVC), identified in peanut (Arachis hypogaea). The three segmented genomic RNA molecules of PVC were 3474 (RNA1), 2925 (RNA2), and 2160 (RNA3) nucleotides in length, with five predicted open reading frames containing conserved domains and motifs that are typical features of ilarviruses. The three genomic RNAs shared nucleotide sequence similarity (74% identity and 93% query coverage for RNA1, 75% identity and 85% query coverage for RNA2, and 72% identity and 70% query coverage for RNA3) with the most closely related ilarvirus, parietaria mottle virus...
April 12, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29651716/super-secondary-structure-consisting-of-a-polyproline-ii-helix-and-a-%C3%AE-turn-in-leucine-rich-repeats-in-bacterial-type-iii-secretion-system-effectors
#16
Dashdavaa Batkhishig, Khurelbaatar Bilguun, Purevjav Enkhbayar, Hiroki Miyashita, Robert H Kretsinger, Norio Matsushima
Leucine rich repeats (LRRs) are present in over 100,000 proteins from viruses to eukaryotes. The LRRs are 20-30 residues long and occur in tandem. LRRs form parallel stacks of short β-strands and then assume a super helical arrangement called a solenoid structure. Individual LRRs are separated into highly conserved segment (HCS) with the consensus of LxxLxLxxNxL and variable segment (VS). Eight classes have been recognized. Bacterial LRRs are short and characterized by two prolines in the VS; the consensus is xxLPxLPxx with Nine residues (N-subtype) and xxLPxxLPxx with Ten residues (T-subtype)...
April 12, 2018: Protein Journal
https://www.readbyqxmd.com/read/29651709/-cannabinoid-therapy-in-practice
#17
REVIEW
T Rasche, D Emmert, C Stieber, M Mücke, R Conrad
BACKGROUND: In recent years, the media and scientists have shown increased interest in cannabis-based drugs. OBJECTIVES: Background information about cannabis-based drugs and their mechanism of action as well as discussion of possible applications as supportive therapy or in palliative medicine, respectively, are presented. MATERIALS AND METHODS: The recent literature was examined and evaluated. RESULTS: In many medical fields, we do not have sufficient evidence for the efficacy of cannabinoids...
April 12, 2018: Der Urologe. Ausg. A
https://www.readbyqxmd.com/read/29651117/field-validation-of-recombinant-antigen-immunoassays-for-diagnosis-of-lassa-fever
#18
Matthew L Boisen, Jessica N Hartnett, Jeffrey G Shaffer, Augustine Goba, Mambu Momoh, John Demby Sandi, Mohamed Fullah, Diana K S Nelson, Duane J Bush, Megan M Rowland, Megan L Heinrich, Anatoliy P Koval, Robert W Cross, Kayla G Barnes, Anna E Lachenauer, Aaron E Lin, Mahan Nekoui, Dylan Kotliar, Sarah M Winnicki, Katherine J Siddle, Michael Gbakie, Mbalu Fonnie, Veronica J Koroma, Lansana Kanneh, Peter C Kulakosky, Kathryn M Hastie, Russell B Wilson, Kristian G Andersen, Onikepe O Folarin, Christian T Happi, Pardis C Sabeti, Thomas W Geisbert, Erica Ollmann Saphire, S Humarr Khan, Donald S Grant, John S Schieffelin, Luis M Branco, Robert F Garry
Lassa fever, a hemorrhagic fever caused by Lassa virus (LASV), is endemic in West Africa. It is difficult to distinguish febrile illnesses that are common in West Africa from Lassa fever based solely on a patient's clinical presentation. The field performance of recombinant antigen-based Lassa fever immunoassays was compared to that of quantitative polymerase chain assays (qPCRs) using samples from subjects meeting the case definition of Lassa fever presenting to Kenema Government Hospital in Sierra Leone. The recombinant Lassa virus (ReLASV) enzyme-linked immunosorbant assay (ELISA) for detection of viral antigen in blood performed with 95% sensitivity and 97% specificity using a diagnostic standard that combined results of the immunoassays and qPCR...
April 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29650854/-a-case-of-hyperammonemia-induced-by-chemotherapy-with-5-fluorouracil-for-metastatic-colon-cancer
#19
Shinji Tokuyama, Mutsumi Fukunaga, Ken Konishi, Shoko Honda, Ryohei Yukimoto, Aoi Okamoto, Akina Saito, Kazuyuki Okada, Hideo Ota, Shigekazu Yokoyama, Hirofumi Miki, Kenji Kobayashi
Systemic chemotherapy based on 5-fluorouracil(5-FU)is a standard treatment for unresectable or recurrent colon cancers. Here, we report a case of hyperammonemia induced by chemotherapy using 5-FU for metastatic colon cancer. An 84-yearold male patient with past histories of liver cirrhosis related to hepatitis C virus and renal dysfunction underwent an operation for the rectosigmoid colon cancer 8 years ago. Three years after that operation, a local recurrence of the colon cancer was diagnosed, and chemotherapy using sLV5FU2 was initiated...
April 2018: Gan to Kagaku Ryoho. Cancer & Chemotherapy
https://www.readbyqxmd.com/read/29650672/tropism-for-tuft-cells-determines-immune-promotion-of-norovirus-pathogenesis
#20
Craig B Wilen, Sanghyun Lee, Leon L Hsieh, Robert C Orchard, Chandni Desai, Barry L Hykes, Michael R McAllaster, Dale R Balce, Taylor Feehley, Jonathan R Brestoff, Christina A Hickey, Christine C Yokoyama, Ya-Ting Wang, Donna A MacDuff, Darren Kreamalmayer, Michael R Howitt, Jessica A Neil, Ken Cadwell, Paul M Allen, Scott A Handley, Menno van Lookeren Campagne, Megan T Baldridge, Herbert W Virgin
Complex interactions between host immunity and the microbiome regulate norovirus infection. However, the mechanism of host immune promotion of enteric virus infection remains obscure. The cellular tropism of noroviruses is also unknown. Recently, we identified CD300lf as a murine norovirus (MNoV) receptor. In this study, we have shown that tuft cells, a rare type of intestinal epithelial cell, express CD300lf and are the target cell for MNoV in the mouse intestine. We found that type 2 cytokines, which induce tuft cell proliferation, promote MNoV infection in vivo...
April 13, 2018: Science
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