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Sedef Ilk, Necdet Sağlam, Mustafa Özgen, Feza Korkusuz
Quorum sensing (QS) is a cell density dependent expression of species in bacteria mediated by compounds called autoinducers (AI). Several processes responsible for successful establishment of bacterial infection are mediated by QS. Inhibition of QS is therefore being considered as a new target for antimicrobial chemotherapy. Flavonoid compounds are strong antioxidant and antimicrobial agents but their applications are limited due to their poor dissolution and bioavailability. Our objective was to investigate the effect of kaempferol loaded chitosan nanoparticles on modulating QS mediated by AI in model bioassay test systems...
October 21, 2016: International Journal of Biological Macromolecules
Fahmy A Mamuya, Jose Luis Cano-Peñalver, Wei U Li, Diego Rodriguez-Puyol, Manuel Rodríguez-Puyol, Dennis Brown, Sergio de Frutos, Hua Jenny Lu
Within the past decade tremendous efforts have been made to understand the mechanism behind aquaporin-2 (AQP2) water channel trafficking and recycling, in order to open a path towards effective diabetes insipidus therapeutics. A recent study has shown that Integrin-Linked Kinase (ILK) conditional-knockdown mice developed polyuria along with decreased expression of AQP2. To understand whether ILK also regulates AQP2 trafficking in kidney tubular cells, we performed in vitro analysis using LLCPK1 cells stably expressing rat AQP2 (LLC-AQP2 cells)...
October 19, 2016: American Journal of Physiology. Renal Physiology
Gwendalyn J Randolph, Shashi Bala, Jean-François Rahier, Michael W Johnson, Peter L Wang, ILKe Nalbantoglu, Laurent Dubuquoy, Amélie Chau, Benjamin Pariente, Alex Kartheuser, Bernd H Zinselmeyer, Jean-Frederic Colombel
Early pathological descriptions of Crohn disease (CD) argued for a potential defect in lymph transport; however, this concept has not been thoroughly investigated. In mice, poor healing in response to infection-induced tissue damage can cause hyperpermeable lymphatic collecting vessels in mesenteric adipose tissue that impair antigen and immune cell access to mesenteric lymph nodes (LNs), which normally sustain appropriate immunity. To investigate whether analogous changes might occur in human intestinal disease, we established a three-dimensional imaging approach to characterize the lymphatic vasculature in mesenteric tissue from controls or patients with CD...
October 13, 2016: American Journal of Pathology
Yi Li, Xiaoli Shi, Lei Tian, Hongyu Sun, Yujing Wu, Xia Li, Jianjun Li, Yujie Wei, Xinxiao Han, Jiao Zhang, Xiaowei Jia, Rui Bai, Limin Jing, Peng Ding, Huiliang Liu, Dong Han
A schematic for the mechanism of accelerating the assembly of intercalated discs (IDs) in cardiac myocytes regulated by gold nanoparticles (AuNPs) is presented. AuNPs with local nanoscale stiffness in the substrate activate β1-integrin signaling, which mediates the activation of integrin-linked kinase (ILK) and its downstream signal kinase by stimulating expression of the transcription factors GATA4 and MEF-2c.
October 10, 2016: Advanced Materials
Ming-Xing Wang, Xiao-Juan Zhao, Tian-Yu Chen, Yang-Liu Liu, Rui-Qing Jiao, Jian-Hua Zhang, Chunhua Ma, Jia-Hui Liu, Ying Pan, Ling-Dong Kong
Nuciferine is a major active component from lotus leaf. This study examined the effects of nuciferine on fructose-induced renal injury and explored its possible mechanism. Rats consumed drinking water or 10% fructose for 12 wk. Fructose-fed rats were orally treated with water, or 7, 14 or 28 mg/kg nuciferine for the last 6 wk. HK-2 cells were exposed to 5 mM fructose alone or in combination with nuciferine (2.5-40 μM) for 24 h. Nuciferine significantly attenuated fructose-induced hyperuricemia, dyslipidemia and systemic inflammation in rats...
October 10, 2016: Journal of Agricultural and Food Chemistry
Anna Skoczyńska, Elżbieta Budzisz, Kasjana Podgórna, Helena Rotsztejn
Morphology of senescent cells is constantly changing at the molecular level, which in turn leads to disruption of their function. It is connected with reduced ability to synthesize extracellular matrix (ECM) and leads to the dysfunction of integrin adhesion molecules and adhesion clusters. In skin, these factors cause a loss of communication between the extracellular matrix and fibroblasts. This contributes to the appearance of signs of aging. The aim of this study is to draw attention to the very important molecule such as paxillin, which is an adaptor protein with mass of 68 kDa...
October 6, 2016: Postȩpy Higieny i Medycyny Doświadczalnej
Dorota Gil, Dorota Ciołczyk-Wierzbicka, Joanna Dulińska-Litewka, Piotr Laidler
Cadherin switch is specific of epithelial-mesenchymal transition (EMT) and is closely related to tumor cell invasion. However, the molecular mechanism that promotes the phenotypic changes remains unclear and elusive. We found that integrin-linked kinase (ILK) is a key factor involved in cadherin switch. The expression and activity of ILK are elevated in a variety of cancers but its mechanisms are not exactly understood. In this report, we studied the role and mechanism of ILK in EMT of human bladder cancer...
September 28, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Izabela Papiewska-Pająk, Maria A Kowalska, Joanna Boncela
Inhibition of E-cadherin gene expression by transcription factor SNAIL is known to be a crucial element of Epithelial to Mesenchymal Transition; EMT. Epigenetic regulation of E-cadherin expression is regulated by SNAIL binding to E-box sequences in the CDH1 gene promoter and recruiting enzymes belonging to repressor complexes that are directly engaged in histone modifications and DNA methylation leading to the modification of chromatin structure. SNAIL involvement in cell acquisition of invasive phenotype is based on direct suppression of tight-junction and gap junction proteins...
2016: Postȩpy Higieny i Medycyny Doświadczalnej
M E Mercado-Pimentel, S Igarashi, A M Dunn, M Behbahani, C Miller, C M Read, A Jacob
Activation of PKB/AKT signaling, which requires PDK1 and PDK2 function, drives Vestibular Schwannoma (VS) and meningioma growth. PDK2 function is defined as a molecule that phosphorylates AKT-Ser473. Integrin-Linked Kinase (ILK) functions as PDK2 in PKB/AKT activation in many cancers; therefore, we hypothesized that OSU-T315, a small molecule ILK inhibitor, will inhibit the ILK-PDK2 function in PKB/AKT signaling activation in VS and meningioma cell growth. OSU-T315 decreased cell viability at IC50 < 2μM in VS (HEI193) and meningioma (Ben-Men-1) cell lines, in primary cells at < 3...
2016: Austin J Med Oncol
Ernest Ho, Iordanka A Ivanova, Lina Dagnino
The formation of tight cell-cell junctions is essential in the epidermis for its barrier properties. In this tissue, keratinocytes follow a differentiation program tightly associated with their movement from the innermost basal to the outer suprabasal layers, and with changes in their cell-cell adhesion profile. Intercellular adhesion in keratinocytes is mediated through cell-cell contacts, including E-cadherin-based adherens junctions. Although the mechanisms that mediate E-cadherin delivery to the plasma membrane have been widely studied in simple epithelia, this process is less well understood in the stratified epidermis...
September 11, 2016: Biochimica et Biophysica Acta
Lukáš Nosek, Dmitry Semchonok, Egbert J Boekema, Petr Ilík, Roman Kouřil
Plant photosystem II (PSII) is organized into large supercomplexes with variable amount of membrane-bound light-harvesting proteins (LHCII). The largest stable form of the PSII supercomplex involves four LHCII trimers, which are specifically connected to the PSII core dimer via monomeric antenna proteins. The PSII supercomplexes can further interact in thylakoid membrane, forming PSII megacomplexes. So far, only megacomplexes consisting of two PSII supercomplexes associated in parallel have been observed. Here we show that the forms of PSII megacomplexes can be much more variable...
September 6, 2016: Plant Journal: for Cell and Molecular Biology
Barbara Pucelik, Ilke Gürol, Vefa Ahsen, Fabienne Dumoulin, Janusz M Dąbrowski
A fluorinated phthalocyanine and its non-fluorinated analogue were selected to evaluate the potential enhancement of fluorination on photophysical, photochemical and redox properties as well as on biological activity in cellular and animal models. Due to the pharmacological relevance, the affinity of these phthalocyanines towards biological membranes (logPow) as well as their primary interaction with human serum albumin (HSA) or low-density lipoprotein (LDL) were determined. Water-dispersible drug formulation of phthalocyanines via Pluronic(®)-based triblock copolymer micelles was prepared to avoid self-aggregation effects and to improve their delivery...
August 20, 2016: European Journal of Medicinal Chemistry
Z Y Wang, W Zhang, J J Yang, D K Song, J X Wei, S Gao
The clinical significance of thymosin β4 (Tβ4) expression in bladder transitional cell carcinoma (BTCC) remains unclear. The present study assessed the relationship between the expression of Tβ4 protein and the clinicopathological features, as well as the prognosis of bladder cancer patients. Tβ4 protein expression in 24 normal bladder and 138 primary BTCC tissue specimens was detected by immunohistochemistry, and the association of this expression with BTCC clinicopathological features and recurrence as well as patient survival was analyzed...
September 6, 2016: Neoplasma
Mehmet Ilke Buget, Ata Can Atalar, Ipek Saadet Edipoglu, Zerrin Sungur, Nukhet Sivrikoz, Meltem Karadeniz, Esra Saka, Suleyman Kucukay, Mert N Senturk
BACKGROUND AND OBJECTIVES: The aim of the study were to demonstrate the possible hemodynamic changes and cerebral blood flow alterations in patients who were positioned from supine to beach chair position; and to detect if the position change causes any cortical activity alteration as measured by the 4-channeled electroencephalography monitor. METHODS: 35 patients were included. Before the induction, mean arterial pressure and patient state index values were recorded (T0)...
September 2016: Brazilian Journal of Anesthesiology
Huamin Xu, Huiling Cao, Guozhi Xiao
Particularly interesting new cysteine-histidine-rich protein (PINCH) is a LIM-domain-only adaptor that plays important roles in cytoskeletal organization and extracellular matrix adhesion, migration, proliferation and survival. Mammalian cells have two functional PINCH proteins, PINCH1 and PINCH2. PINCH not only binds to Nck2 and engages in the signaling of growth factor receptors, but also forms a ternary complex with ILK and parvin (IPP complex). Normally, the IPP complex locates to focal adhesions participating in the signaling of integrins and mediating the interaction of cytoskeleton and extracellular matrix (ECM)...
December 5, 2016: Gene
Xiang Zhuang, Mengxin Lv, Zhenyu Zhong, Luyu Zhang, Rong Jiang, Junxia Chen
BACKGROUND: Integrin-linked kinase (ILK) is a multifunctional adaptor protein which is involved with protein signalling within cells to modulate malignant (cancer) cell movement, cell cycle, metastasis and epithelial-mesenchymal transition (EMT). Our previous experiment demonstrated that ILK siRNA inhibited the growth and induced apoptosis of bladder cancer cells as well as increased the expression of Ribonuclease inhibitor (RI), an important cytoplasmic protein with many functions. We also reported that RI overexpression inhibited ILK and phosphorylation of AKT and GSK3β...
2016: Journal of Experimental & Clinical Cancer Research: CR
Mitra L Taheri, Eric A Stach, Ilke Arslan, P A Crozier, Bernd C Kabius, Thomas LaGrange, Andrew M Minor, Seiji Takeda, Mihaela Tanase, Jakob B Wagner, Renu Sharma
This review article discusses the current and future possibilities for the application of in situ transmission electron microscopy to reveal synthesis pathways and functional mechanisms in complex and nanoscale materials. The findings of a group of scientists, representing academia, government labs and private sector entities (predominantly commercial vendors) during a workshop, held at the Center for Nanoscale Science and Technology- National Institute of Science and Technology (CNST-NIST), are discussed. We provide a comprehensive review of the scientific needs and future instrument and technique developments required to meet them...
August 6, 2016: Ultramicroscopy
Jennifer L Peotter, Jenny Phillips, Tiegang Tong, Kaylee Dimeo, Jose M Gonzalez, Donna M Peters
We previously demonstrated that an αvβ5 integrin/FAK- mediated pathway regulated the phagocytic properties of human trabecular meshwork (HTM) cells. Here we demonstrate that this process is mediated by Rac-1 and a previously unreported signaling pathway that utilizes the Tiam1 as well as a novel ILK/RhoG/ELMO2 signaling pathway. Phagocytosis in both a TM-1 cell line and normal HTM cells was mediated by Rac1 and could be significantly decreased by >75% using the Rac1 inhibitor EHop-016. Knockdown of Rac1 in TM-1 cells also inhibited phagocytosis by 40% whereas overexpression of a constitutively active Rac1 or stimulation with PDGF increased phagocytosis by 83% and 32% respectively...
October 1, 2016: Experimental Cell Research
Chang-Fang Chiu, Jing-Ru Weng, Appaso Jadhav, Chia-Yung Wu, Aaron M Sargeant, Li-Yuan Bai
T315, an integrin-linked kinase (ILK) inhibitor, has been shown to suppress the proliferation of breast cancer, stomach cancer and chronic lymphocytic leukemia cells. Here we demonstrate that T315 decreases cell viability of acute myeloid leukemia (AML) cell lines (HL-60 and THP-1) and primary leukemia cells from AML patients in a dose-responsive manner. Normal human bone marrow cells are less sensitive than leukemia cells to T315. T315 down regulates protein kinase B (Akt) and p-Akt and induces caspase activation, poly-ADP-ribose polymerase (PARP) cleavage, apoptosis and autophagy through an ILK-independent manner...
2016: International Journal of Molecular Sciences
M H Hwang, K H Cho, K J Jeong, Y-Y Park, J M Kim, S-L Yu, C G Park, G B Mills, H Y Lee
Rab coupling protein (RCP)-induced tumor cell migration has been implicated in tumor pathophysiology and patient outcomes. In the present study, we demonstrate that RCP stabilizes β1 integrin leading to increased β1 integrin levels and activation of a signaling cascade culminating in Slug induction, epithelial-to-mesenchymal transition and increased invasion. Ectopic expression of RCP induced Slug expression. Silencing β1 integrin efficiently inhibited RCP-induced Slug expression and subsequent cancer cell invasion...
August 15, 2016: Oncogene
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