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Arthritis micelle

Takanori Kanazawa, Takahiro Endo, Naoki Arima, Hisako Ibaraki, Yuuki Takashima, Yasuo Seta
This study aimed to build an innovative system to deliver a systemic small interfering RNA (siRNA) treatment for rheumatoid arthritis. We combined arginine-histidine-cysteine based oligopeptide-modified polymer micelles with siRNA targeting the nuclear factor κB subunit, RelA (siRelA). This is a key molecule in the control of inflammation. We tested the cellular uptake of siRNA and its effects on inflammatory cytokine levels in vitro using synoviocytes, and siRNA distribution and therapeutic effects in vivo in mice with collagen-induced arthritis (CIA)...
October 17, 2016: International Journal of Pharmaceutics
Mariam Mohammadi, Ying Li, Daniel G Abebe, Yuran Xie, Rima Kandil, Teresa Kraus, Nardhy Gomez-Lopez, Tomoko Fujiwara, Olivia M Merkel
New folic acid (FA) coupled three layered micelles (3LM) were designed to encapsulate DNA, and their application as delivery system that specifically targets activated macrophages was investigated for new treatment options in rheumatoid arthritis (RA). FA coupled poly(l-lactide)-b-poly(ethylene glycol) (FA-PEG-PLLA) was synthesized via the NHS-ester activated/amine coupling method. Fluorescein labeled folic acid was used for flow cytometric detection of the expression of functional folic receptor β in LPS-activated and resting macrophages...
August 23, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
Iris E Allijn, Raymond M Schiffelers, Gert Storm
Curcumin, originally used in traditional medicine and as a spice, is one of the most studied and most popular natural products of the past decade. It has been described to be an effective anti-inflammatory and anti-cancer drug and protects against chronic diseases such as rheumatoid arthritis and atherosclerosis. Despite these promising pharmacological properties, curcumin is also very lipophilic, which makes its formulation challenging. Ideally the nanocarrier should additionally also retain the encapsulated curcumin to provide target tissue accumulation...
June 15, 2016: International Journal of Pharmaceutics
Qin Wang, Jiayu Jiang, Wenfei Chen, Hao Jiang, Zhirong Zhang, Xun Sun
Glucocorticoid (GC) is the cornerstone therapy of rheumatoid arthritis, but high doses are associated with serious adverse effects. In an effort to improve the efficacy of low-dose GC therapy, we developed a micelle system for targeted delivery to inflamed joints and validated the approach in a rat model of arthritis. Micelles loaded with dexamethasone (Dex) self-assembled from the amphipathic poly (ethylene glycol)-block-poly (ε-caprolactone) (PCL-PEG) polymer via film dispersion, and they were injected intravenously at a dose of only 0...
May 28, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
Thavasyappan Thambi, Soyoung Son, Doo Sung Lee, Jae Hyung Park
UNLABELLED: Hypoxia occurs in a variety of pathological conditions including stroke, rheumatoid arthritis, atherosclerosis, and tumors. In this study, an amphiphilic block copolymer, composed of poly(ethylene glycol) as the hydrophilic block and poly(ε-(4-nitro)benzyloxycarbonyl-L-lysine) as the hydrophobic block, was prepared for hypoxia-sensitive drug delivery. Owing to its amphiphilic nature, the block copolymer formed micelles and encapsulated doxorubicin (DOX) in an aqueous condition...
January 2016: Acta Biomaterialia
Yingjuan Lu, Nikki Parker, Paul J Kleindl, Vicky A Cross, Kristin Wollak, Elaine Westrick, Torian W Stinnette, Mark A Gehrke, Kevin Wang, Hari Krishna R Santhapuram, Fei You, Spencer J Hahn, Jeremy F Vaughn, Patrick J Klein, Iontcho R Vlahov, Philip S Low, Christopher P Leamon
Folate receptor (FR)-β has been identified as a promising target for antimacrophage and antiinflammatory therapies. In the present study, we investigated EC0565, a folic acid-derivative of everolimus, as a FR-specific inhibitor of the mammalian target of rapamycin (mTOR). Because of its amphiphilic nature, EC0565 was first evaluated for water solubility, critical micelle formation, stability in culture and FR-binding specificity. Using FR-expressing macrophages, the effect of EC0565 on mTOR signaling and cellular proliferation was studied...
2015: Molecular Medicine
Bálint Botz, Kata Bölcskei, Ágnes Kemény, Zoltán Sándor, Valéria Tékus, György Sétáló, Janka Csepregi, Attila Mócsai, Erika Pintér, László Kollár, Zsuzsanna Helyes
Vascular leakage is an important feature of various disease conditions. In vivo optical imaging provides a great opportunity for the evaluation of this phenomenon. In the present study, we focus on the development and validation of a near-infrared (NIR) imaging formula to allow reliable, cost-efficient evaluation of vascular leakage in diverse species using the existing small-animal fluorescence imaging technology. IR-676, a moderately hydrophobic NIR cyanine dye, was doped into self-assembling aqueous micelles using a widely employed and safe nonionic emulsifier (Kolliphor HS 15), and was tested in several acute and chronic inflammatory disease models in both mice and rats...
January 2015: Journal of Biomedical Optics
Roger New, Gurpal S Bansal, Malgorzata Dryjska, Michal Bogus, Patricia Green, Marc Feldmann, Fionula Brennan
Although strong binding interactions between protein receptor and ligand do not require the participation of a large number of amino acids in either site, short peptide chains are generally poor at recreating the types of protein-protein interactions which take place during cell recognition and signalling process, probably because their flexible backbones prevent the side chains from forming sufficiently rigid and stable epitopes, which can take part in binding with the desired strength and specificity. In a recently-reported study, it was shown that a proto-epitope containing F, R and S amino acids has the ability to down-regulate TNF secretion by macrophages...
2014: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
C M Spies, E Stemmler, F Buttgereit
Results of the CONDOR study suggest that in osteoarthritis and rheumatoid arthritis patients at elevated risk of gastrointestinal (GI) events, treatment with celecoxib, a cyclooxygenase (COX)-2 selective non-steroidal anti-inflammatory drug (NSAID), demonstrated significantly lower toxicity in the upper and lower (GI) tract when compared to the non-selective NSAID diclofenac plus a proton-pump-inhibitor (PPI), omeprazole. According to current knowledge, traditional NSAIDs (tNSAIDs) as non-selective COX-inhibitors exert their damaging effects on the upper GI tract, largely by reduction of the COX-1 related synthesis of gastro-protective prostaglandins...
January 2015: Medical Hypotheses
Diego R Perinelli, Giulia Bonacucina, Stefania Pucciarelli, Marco Cespi, Luca Casettari, Valeria Polzonetti, Francesco Martino Carpi, Giovanni F Palmieri
PURPOSE: This work investigated the influence of a model protein, bovine serum albumin (BSA), on the properties of a thermogelling formulation intended for administration inside body compartments where there is high albumin content, as in the case of inflamed joints; it also explored the relation between the variation of these properties and release performance of methotrexate (MTX), a drug used to treat forms of arthritis and rheumatic conditions. METHODS: The influence of BSA on the micellisation and gelation behaviour of Poloxamer 407, chosen as a model copolymer, was studied by differential scanning calorimetry (microDSC), dynamic light scattering (DLS), fluorescence spectroscopy and rheology studies...
March 2015: Pharmaceutical Research
George Kokotos, Astrid J Feuerherm, Efrosini Barbayianni, Ishita Shah, Mari Sæther, Victoria Magrioti, Thuy Nguyen, Violetta Constantinou-Kokotou, Edward A Dennis, Berit Johansen
Group IVA cytosolic phospholipase A2 (GIVA cPLA2) is the rate-limiting provider of pro-inflammatory mediators in many tissues and is thus an attractive target for the development of novel anti-inflammatory agents. In this work, we present the synthesis of new thiazolyl ketones and the study of their activities in vitro, in cells, and in vivo. Within this series of compounds, methyl 2-(2-(4-octylphenoxy)acetyl)thiazole-4-carboxylate (GK470) was found to be the most potent inhibitor of GIVA cPLA2, exhibiting an XI(50) value of 0...
September 25, 2014: Journal of Medicinal Chemistry
Lingdong Quan, Yijia Zhang, Bart J Crielaard, Anand Dusad, Subodh M Lele, Cristianne J F Rijcken, Josbert M Metselaar, Hana Kostková, Tomáš Etrych, Karel Ulbrich, Fabian Kiessling, Ted R Mikuls, Wim E Hennink, Gert Storm, Twan Lammers, Dong Wang
As an emerging research direction, nanomedicine has been increasingly utilized to treat inflammatory diseases. In this head-to-head comparison study, four established nanomedicine formulations of dexamethasone, including liposomes (L-Dex), core-cross-linked micelles (M-Dex), slow releasing polymeric prodrugs (P-Dex-slow), and fast releasing polymeric prodrugs (P-Dex-fast), were evaluated in an adjuvant-induced arthritis rat model with an equivalent dose treatment design. It was found that after a single i.v...
January 28, 2014: ACS Nano
Min Ley Pua, Toru Yoshitomi, Pennapa Chonpathompikunlert, Aki Hirayama, Yukio Nagasaki
Reactive oxygen species (ROS) scavengers have not been widely used for treatment of local inflammatory reactions such as arthritis and periodontal disease because they are rapidly eliminated from the inflamed site, which results in a low therapeutic effect. Therefore, to enhance the local retention time of ROS scavengers, we developed a redox-active injectable gel (RIG) system by using poly[4-(2,2,6,6-tetramethylpiperidine-N-oxyl)aminomethylstyrene]-b-poly(ethylene glycol)-b-poly[4-(2,2,6,6-tetramethylpiperidine-N-oxyl)aminomethylstyrene] (PMNT-PEG-PMNT) triblock copolymer, which possesses ROS scavenging nitroxide radicals as side chains of the PMNT segment...
December 28, 2013: Journal of Controlled Release: Official Journal of the Controlled Release Society
David R Wilson, Nan Zhang, Angela L Silvers, Martin B Forstner, Rebecca A Bader
Polysialic acid (PSA) has been identified as a natural, hydrophilic polymer that can be used to extend circulation time and improve therapeutic efficacy when used as the basis of drug carrier systems. Here, to further investigate the potential of PSA to alter the pharmacokinetic and pharmacodynamic profiles of associated therapeutics, PSA-based micelles were formed via self-assembly of PSA grafted with polycaprolactone (PCL) at a critical micelle concentration of 84.7±13.2 μg/ml. Cyclosporine A (CyA), a therapeutic used in the treatment of rheumatoid arthritis, was loaded into the PSA-PCL micelles with a loading capacity and loading efficiency of 0...
January 23, 2014: European Journal of Pharmaceutical Sciences
Xiawei Wei, Xinyi Lv, Qinghe Zhao, Liyan Qiu
A novel biodegradable and injectable in situ gel-forming controlled drug delivery system based on thermosensitive β-cyclodextrin-modified poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) co-polymer (PCEC-β-CD) was studied in this work. The drug encapsulating capacity has been improved by introducing β-CD bound to the PCEC co-polymer. The prepared PCEC-β-CD co-polymers self-assembled in water to form micelles, and underwent a temperature-dependent gel-sol transition, which was in the form of a flowing injectable solution at low temperatures but became a non-flowing gel at around physiological body temperature...
June 2013: Acta Biomaterialia
Samira Sadat Abolmaali, Ali Mohammad Tamaddon, Rassoul Dinarvand
PURPOSE: Methotrexate (MTX) is one of the most widely studied and effective therapeutics agents available to treat many solid tumors, hematologic malignancies, and autoimmune diseases such as rheumatoid arthritis; however, the poor pharmacokinetic and narrow safety margin of the drug limits the therapeutic outcomes of conventional drug delivery systems. For an improved delivery of MTX, several pathophysiological features such as angiogenesis, enhanced permeability and retention effects, acidosis, and expression of specific antigens and receptors can be used either as targets or as tools for drug delivery...
May 2013: Cancer Chemotherapy and Pharmacology
Varun Sethi, Israel Rubinstein, Antonina Kuzmis, Helen Kastrissios, James Artwohl, Hayat Onyuksel
Despite advances in rheumatoid arthritis (RA) treatment, efficacious and safe disease-modifying therapy still represents an unmet medical need. Here, we describe an innovative strategy to treat RA by targeting low doses of vasoactive intestinal peptide (VIP) self-associated with sterically stabilized micelles (SSMs). This spontaneous interaction of VIP with SSM protects the peptide from degradation or inactivation in biological fluids and prolongs circulation half-life. Treatment with targeted low doses of nanosized SSM-VIP but not free VIP in buffer significantly reduced the incidence and severity of arthritis in an experimental model, completely abrogating joint swelling and destruction of cartilage and bone...
February 4, 2013: Molecular Pharmaceutics
Jieying Liu, Siyuan Chen, Li Lv, Lei Song, Shengrong Guo, Shengtang Huang
A hydrophobic polyphenol compound extracted from turmeric, curcumin has been widely utilized as traditional medicines for centuries in China and India. Over the last decades, because of its low toxicity, extensive studies have been focused on its physicochemical properties and pharmacological activities on various diseases, such as cancer, cardio-vascular disease, inflammatory bowel, wound healing, Alzheimer's disease, rheumatoid arthritis, and diabetes. In particular, bioactivities of curcumin as an effective chemopreventive agent, chemo-/radio-sensitizer for tumor cells, and chemo-/radio-protector for normal organs, are of extraordinary research interests in the literature...
2013: Current Pharmaceutical Design
Bart J Crielaard, Cristianne J F Rijcken, Lingdong Quan, Steffen van der Wal, Isil Altintas, Martin van der Pot, John A W Kruijtzer, Rob M J Liskamp, Raymond M Schiffelers, Cornelus F van Nostrum, Wim E Hennink, Dong Wang, Twan Lammers, Gert Storm
Polymerizable and hydrolytically cleavable dexamethasone (DEX, red dot in picture) derivatives were covalently entrapped in core-cross-linked polymeric micelles that were prepared from a thermosensitive block copolymer (yellow and gray building block). By varying the oxidation degree of the thioether in the drug linker, the release rate of DEX could be controlled. The DEX-loaded micelles were used for efficient treatment of inflammatory arthritis in two animal models.
July 16, 2012: Angewandte Chemie
Michal Bachar, Amitai Mandelbaum, Irina Portnaya, Hadas Perlstein, Simcha Even-Chen, Yechezkel Barenholz, Dganit Danino
β-casein is an amphiphilic protein that self-organizes into well-defined core-shell micelles. We developed these micelles as efficient nanocarriers for oral drug delivery. Our model drug is celecoxib, an anti-inflammatory hydrophobic drug utilized for treatment of rheumatoid arthritis and osteoarthritis, now also evaluated as a potent anticancer drug. This system is unique as it enables encapsulation loads >100-fold higher than other β-casein/drug formulations, and does not require additives as do other formulations that have high loadings...
June 10, 2012: Journal of Controlled Release: Official Journal of the Controlled Release Society
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