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image analysis and processing

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https://www.readbyqxmd.com/read/29334223/a-novel-color-modulation-analysis-strategy-through-tunable-multiband-laser-for-nanoparticle-identification-and-evaluation
#1
Xuan Cao, Gang Lei, Jingjing Feng, Qi Pan, Xiaodong Wen, Yan He
Creating color difference and improving the color resolution in digital imaging is crucial for better application of color analy-sis. Herein, a novel color modulation analysis strategy was developed by using a homemade tunable multiband laser illumi-nation device, in which the portions of R, G, and B components of the illumination light are discretionarily adjustable, and hence the sample color could be visually modulated continuously in the RGB color space. Through this strategy, the color appearance of single gold nanorods (AuNRs) under dark-field microscopy, was migrated from the spectrally insensitive red region to the spectrally sensitive green-yellow-red region...
January 15, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29334131/a-bayesian-method-for-accelerated-magnetic-resonance-elastography-of-the-liver
#2
Christopher Ebersole, Rizwan Ahmad, Adam V Rich, Lee C Potter, Huiming Dong, Arunark Kolipaka
PURPOSE: Magnetic resonance elastography is a noninvasive tool for quantifying soft tissue stiffness. Magnetic resonance elastography has been adopted as a clinical method for staging liver fibrosis. However, the application of liver magnetic resonance elastography requires multiple lengthy breath-holds. We propose a new data acquisition and processing method to reduce magnetic resonance elastography scan time. METHODS: A Bayesian image reconstruction method that uses transform sparsity and magnitude consistency across different phase offsets to recover images from highly undersampled data is proposed...
January 15, 2018: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29333754/a-guide-to-analysis-and-reconstruction-of-serial-block-face-scanning-electron-microscopy-data
#3
E Cocks, M Taggart, F C Rind, K White
Serial block face scanning electron microscopy (SBF-SEM) is a relatively new technique that allows the acquisition of serially sectioned, imaged and digitally aligned ultrastructural data. There is a wealth of information that can be obtained from the resulting image stacks but this presents a new challenge for researchers - how to computationally analyse and make best use of the large datasets produced. One approach is to reconstruct structures and features of interest in 3D. However, the software programmes can appear overwhelming, time-consuming and not intuitive for those new to image analysis...
January 15, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29333674/microrna-detection-by-dna-mediated-liposome-fusion
#4
Coline Jumeaux, Olov Wahlsten, Stephan Block, Eunjung Kim, Rona Chandrawati, Philip D Howes, Fredrik Höök, Molly M Stevens
Membrane fusion is a process of fundamental importance in biological systems that involves highly selective recognition mechanisms for the trafficking of molecular and ionic cargos. Mimicking natural membrane fusion mechanisms for the purpose of biosensor development holds great potential for amplified detection because relatively few highly discriminating targets lead to fusion and an accompanied engagement of a large payload of signal-generating molecules. In this work, sequence-specific DNA-mediated liposome fusion is used for the highly selective detection of microRNA...
December 7, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29332926/differentiating-between-alzheimer-disease-patients-and-controls-with-phase-difference-enhanced-imaging-at-3t-a-feasibility-study
#5
Machiko Tateishi, Mika Kitajima, Toshinori Hirai, Tetsuya Yoneda, Mamoru Hashimoto, Nan Kurehana, Hiroyuki Uetani, Ryuji Fukuhara, Minako Azuma, Yasuyuki Yamashita
PURPOSE: To test the feasibility of the phase difference enhanced (PADRE) imaging for differentiation between Alzheimer disease (AD) patients and control subjects on 3T MR imaging. MATERIALS AND METHODS: Fifteen patients with AD and 10 age-matched control subjects underwent two-dimensional fast field echo imaging to obtain PADRE images on a 3T MR scanner. A double Gaussian distribution model was used to determine the threshold phase value for differentiation between the physiologic and non-physiologic iron in the cerebral cortices, and PADRE images were processed with the threshold...
January 15, 2018: Magnetic Resonance in Medical Sciences: MRMS
https://www.readbyqxmd.com/read/29332822/constituting-fully-integrated-colorimetric-analysis-system-for-fe-iii-on-multifunctional-nitrogen-doped-moo3-cellulose-paper
#6
Fan Lin, Jiabai Cai, Yuehai Li, Huiwu Yu, Shunxing Li
Using ammonium molybdate and thiourea as precursors, nitrogen-doped MoO3 was produced by a one-step carbonization and then fixed into the cellulose filter paper (NMCP) with acrylic resin as a fixative. NMCP was designed as a multifunctional nanocomposite, i.e., solid phase adsorbent for Fe(III) preconcentration, photocatalyst for iron species transformation and color interference removal, and colorimetric sensor for Fe(III) determination. After photocatalysis, the complex of Fe-humic substances could be transformed into Fe(III) ions, the interference of colored organic matter (e...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29332131/assessment-of-human-corneas-prior-to-transplantation-using-high-resolution-two-photon-imaging
#7
Ana Batista, Hans Georg Breunig, Aisada König, Andreas Schindele, Tobias Hager, Berthold Seitz, António Miguel Morgado, Karsten König
Purpose: The purpose of this study was to evaluate the feasibility of using two-photon imaging (TPI) to assess the condition of human corneas for transplantation. Methods: Human corneas were imaged after different storage times: short-term (STS), medium-term (MTS), and long-term (LTS) storage. A high-resolution, custom-built 5-dimensional multiphoton microscope with 12-fs pulsed laser excitation was used for image acquisition. Results: Optical discrimination between different corneal layers and sublayers based on their morphologic characteristics revealed by two-photon autofluorescence (AF) is possible...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29331739/determination-of-atomic-scale-chemical-composition-at-semiconductor-heteroepitaxial-interfaces-by-high-resolution-transmission-electron-microscopy
#8
C Wen, Y J Ma
The determination of atomic structures and further quantitative information such as chemical compositions at atomic scale for semiconductor defects or heteroepitaxial interfaces can provide direct evidence to understand their formation, modification, and/or effects on the properties of semiconductor films. The commonly used method, high-resolution transmission electron microscopy (HRTEM), suffers from difficulty in acquiring images that correctly show the crystal structure at atomic resolution, because of the limitation in microscope resolution or deviation from the Scherzer-defocus conditions...
January 8, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29331360/fully-automated-segmentation-of-polycystic-kidneys-from-noncontrast-computed-tomography-a-feasibility-study-and-preliminary-results
#9
Dario Turco, Maddalena Valinoti, Eva Maria Martin, Carlo Tagliaferri, Francesco Scolari, Cristiana Corsi
RATIONALE AND OBJECTIVES: Total kidney volume is an important biomarker for the evaluation of autosomal dominant polycystic kidney disease progression. In this study, we present a novel approach for automated segmentation of polycystic kidneys from non-contrast-enhanced computed tomography (CT) images. MATERIALS AND METHODS: Non-contrast-enhanced CT images were acquired from 21 patients with a diagnosis of autosomal dominant polycystic kidney disease. Kidney volumes obtained from the fully automated method were compared to volumes obtained by manual segmentation and evaluated using linear regression and Bland-Altman analyses...
January 10, 2018: Academic Radiology
https://www.readbyqxmd.com/read/29330515/hyphatracker-an-imagej-toolbox-for-time-resolved-analysis-of-spore-germination-in-filamentous-fungi
#10
Michael Brunk, Sebastian Sputh, Sören Doose, Sebastian van de Linde, Ulrich Terpitz
The dynamics of early fungal development and its interference with physiological signals and environmental factors is yet poorly understood. Especially computational analysis tools for the evaluation of the process of early spore germination and germ tube formation are still lacking. For the time-resolved analysis of conidia germination of the filamentous ascomycete Fusarium fujikuroi we developed a straightforward toolbox implemented in ImageJ. It allows for processing of microscopic acquisitions (movies) of conidial germination starting with drift correction and data reduction prior to germling analysis...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330140/exploring-entertainment-medicine-and-professionalization-of-self-care-interview-study-among-doctors-on-the-potential-effects-of-digital-self-tracking
#11
Katleen Gabriels, Tania Moerenhout
BACKGROUND: Nowadays, digital self-tracking devices offer a plethora of possibilities to both healthy and chronically ill users who want to closely examine their body. This study suggests that self-tracking in a private setting will lead to shifting understandings in professional care. To provide more insight into these shifts, this paper seeks to lay bare the promises and challenges of self-tracking while staying close to the everyday professional experience of the physician. OBJECTIVE: The aim of this study was to (1) offer an analysis of how medical doctors evaluate self-tracking methods in their practice and (2) explore the anticipated shifts that digital self-care will bring about in relation to our findings and those of other studies...
January 12, 2018: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/29328311/status-analysis-of-keyhole-bottom-in-laser-mag-hybrid-welding-process
#12
Lin Wang, Xiangdong Gao, Ziqin Chen
The keyhole status is a determining factor of weld quality in laser-metal active gas arc (MAG) hybrid welding process. For a better evaluation of the hybrid welding process, three different penetration welding experiments: partial penetration, normal penetration (or full penetration), and excessive penetration were conducted in this work. The instantaneous visual phenomena including metallic vapor, spatters and keyhole of bottom surface were used to evaluate the keyhole status by a double high-speed camera system...
January 8, 2018: Optics Express
https://www.readbyqxmd.com/read/29328177/projected-image-correction-technology-research-on-autonomous-perception-anisotropic-surfaces
#13
Fan Yang, Cheng Han, Bao-Xing Bai, Chao Zhang
In this paper, a projection correction method is proposed for autonomous-perception depth anisotropic surfaces, in order to improve the adaptive perceptual projection of the projection equipment under different circumstances. During the parameter calibration process of a projector-camera system with low recognition precision, as well as low noise resistance at angular points, an angular-point subpixel detection algorithm based on color information guidance was proposed to effectively improve the identification precision of the angular-point detection...
January 10, 2018: Applied Optics
https://www.readbyqxmd.com/read/29328124/single-shot-3d-topography-of-reflective-samples-with-digital-holographic-microscopy
#14
Raul Castañeda, Jorge Garcia-Sucerquia
In this work, an off-axis digital holographic microscope operating in reflection mode and a telecentric regimen to produce 3D topography of a microscopy sample is shown. The main characteristics of the proposed method, which make it different from the previous works in the field, are the possibility of producing the 3D topography by a single shot over the complete field of view with sensitivity of λ/100, without phase perturbations introduced by the illuminating-imaging system, and with no further numerical processing beyond that required for recovering the phase map of the sample...
January 1, 2018: Applied Optics
https://www.readbyqxmd.com/read/29327966/ocular-histomorphometry-of-free-living-common-kestrels-falco-tinnunculus
#15
Karin Werther, Cinthia Graziela Candioto, Rüdiger Korbel
We evaluated the histomorphometry of the eye structures (cornea, retina, choroid, and sclera) of 13 adult free-living common kestrels ( Falco tinnunculus). Birds included in the study were euthanatized because of severe trauma from a motor vehicle injury. The eyes were enucleated immediately after euthanasia, fixed in 10% buffered formalin, and decalcified. The right eyes were cut vertically (dorsoventrally) and the left eyes cut horizontally (temporonasally). Tissues were processed, stained with hematoxylin and eosin, and evaluated by an image analysis software...
December 2017: Journal of Avian Medicine and Surgery
https://www.readbyqxmd.com/read/29327689/global-spectral-graph-wavelet-signature-for-surface-analysis-of-carpal-bones
#16
Majid Masoumi, Mahsa Rezaei, A Ben Hamza
Quantitative shape comparison is a fundamental problem in computer vision, geometry processing and medical imaging. In this paper, we present a spectral graph wavelet approach for shape analysis of carpal bones of the human wrist. We employ spectral graph wavelets to represent the cortical surface of a carpal bone via the spectral geometric analysis of the Laplace-Beltrami operator in the discrete domain. We propose global spectral graph wavelet (GSGW) descriptor that is isometric invariant, efficient to compute, and combines the advantages of both low-pass and band-pass filters...
January 12, 2018: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29327032/three-dimensional-volumetric-analysis-of-3-fat-processing-techniques-for-facial-fat-grafting-a-randomized-clinical-trial
#17
Rongwei Wu, Xiaonan Yang, Xiaolei Jin, Haibin Lu, Zhenhua Jia, Binghang Li, Haiyue Jiang, Zuoliang Qi
Importance: Autologous fat grafting has revolutionized the field of facial soft-tissue augmentation, despite a lack of standardization. Objective data are needed to arrive at consensus regarding the best technique for optimal volume retention. Objective: To compare 3 fat-processing techniques with 3-dimensional (3-D) technology to explore the optimal fat-processing technique for improving the volume retention of grafted fat. Design, Setting, and Participants: From September 2015 to December 2016, patients with facial asymmetry were treated by initial facial fat grafting at the Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College...
January 11, 2018: JAMA Facial Plastic Surgery
https://www.readbyqxmd.com/read/29326354/accuracy-of-dose-calibrators-for-gallium-68-pet-imaging-unexpected-findings-in-a-multi-centre-clinical-pre-trial-assessment
#18
Dale L Bailey, Michael S Hofman, Nicholas J Forwood, Graeme J O'Keefe, Andrew M Scott, Winifred M van Wyngaardt, Bonnie Howe, Olga Kovacev, Roslyn J Francis
AIMS: We report the discovery of a systematic miscalibration during the work-up process for site validation of a multi-centre clinical PET imaging trial using 68Ga, which manifested as a consistent and reproducible underestimation in the quantitative accuracy (assessed by SUV) of a range of PET cameras from different manufacturers at a number of different facilities around Australia. METHODS: Sites were asked to follow a strict preparation protocol to create a radioactive phantom with 68Ga to be imaged using a standard clinical protocol prior to commencing imaging in the trial...
January 11, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29326176/combinatorial-omics-analysis-reveals-perturbed-lysosomal-homeostasis-in-collagen-vii-deficient-keratinocytes
#19
Kerstin Thriene, Björn Andreas Grüning, Olivier Bornert, Anika Erxleben, Juna Leppert, Ioannis Athanasiou, Ekkehard Weber, Dimitra Kiritsi, Alexander Nyström, Thomas Reinheckel, Rolf Backofen, Cristina Has, Leena Bruckner-Tuderman, Joern Dengjel
The extracellular matrix protein collagen VII is part of the microenvironment of stratified epithelia and critical in organismal homeostasis. Mutations in the encoding gene COL7A1 lead to the skin disorder dystrophic epidermolysis bullosa (DEB), are linked to skin fragility and progressive inflammation-driven fibrosis that facilitates aggressive skin cancer. So far, these changes have been linked to mesenchymal alterations, the epithelial consequences of collagen VII loss remaining under-addressed. As epithelial dysfunction is a principal initiator of fibrosis, we performed a comprehensive transcriptome and proteome profiling of primary human keratinocytes to generate global and detailed images of dysregulated epidermal molecular pathways linked to loss of collagen VII...
January 11, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29325992/quantitative-analysis-of-lipid-debris-accumulation-caused-by-cuprizone-induced-myelin-degradation-in-different-cns-areas
#20
Attila Ozsvár, Róbert Szipőcs, Zoltán Ozsvár, Judith Baka, Pál Barzó, Gábor Tamás, Gábor Molnár
Degradation of myelin sheath is thought to be the cause of neurodegenerative diseases, such as multiple sclerosis (MS), but definitive agreement on mechanism of how myelin is lost is currently lacking. Autoimmune initiation of MS has been recently questioned by proposing that the immune response is a consequence of oligodendrocyte degeneration. To study the process of myelin breakdown, we induced demyelination with cuprizone and applied coherent anti-Stokes Raman scattering (CARS) microscopy, a non-destructive label-free method to image lipid structures in living tissue...
January 8, 2018: Brain Research Bulletin
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