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Jianxin Yang, Feng Ruan, Zhongjun Zheng
BACKGROUND Microvascular endothelial inflammation and apoptosis are responsible for septic acute lung injury (ALI). Ripasudil is a novel Rho/Rho kinase (ROCK) inhibitor which shows therapeutic effects on several vascular diseases. The aim of this study was to investigate the protective effects and correlated molecular mechanisms of ripasudil on lipopolysaccharide- induced inflammation and apoptosis of pulmonary microvascular endothelial cells (PMVECs). MATERIAL AND METHODS Cultured PMVECs were exposed to lipopolysaccharide (LPS)...
May 16, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Guangchao Zhang, Zhe Li, Chao Meng, Jiyu Kang, Mengdi Zhang, Liangjuan Ma, Huacheng Zhou
BACKGROUND: Lung ischemia-reperfusion injury (LIRI) remains an important factor for the early mortality of lung transplantations. Hydrogen (H2) can attenuate lung injury and improve lung function in animal experiments. In previous studies, pulmonary microvascular endothelial cells (PMVECs) were used to simulate LIRI. We hypothesized that H2 can alleviate inflammatory injury in a PMVECs lung transplantation model in the cold ischemia phase. METHODS: PMVECs were divided into 4 groups: Blank, Control, Oxygen (O2), and Hydrogen (H2)...
May 14, 2018: Transplantation
Hongbin Li, Huijuan Shi, Ning Ma, Panpan Zi, Qilong Liu, Rongqing Sun
BML-111 is a lipoxin receptor agonist that plays a vital role on inflammation. MALAT1 is reported to mediate lung injury. ALI rat model was established using the method of venous cannula. Pulmonary microvascular endothelial cells (PMVEC) of rats were isolated using immunomagnetic separation method. Hematoxylin-eosin (HE) staining was performed to observe the lung injury degree. Real-time PCR and western blot were performed to detect the genes expression. ELIAS was used to determine the level of TNF-α and IL-6...
April 25, 2018: Archives of Biochemistry and Biophysics
Xing-Mao Wu, Kai-Qiang Ji, Hai-Yuan Wang, Yang Zhao, Jia Jia, Xiao-Peng Gao, Bin Zang
Severe acute pancreatitis (SAP) is a disease with a high mortality. Patients with SAP may also be complicated with acute lung injury (ALI). So far the therapy for SAP-ALI is still limited. Emerging evidences demonstrate that microRNAs (miRs) could play a role in SAP-ALI. This study aims to define the role of miR-339-3p in SAP-ALI via Anxa3 through the Akt/mTOR signaling pathway. Ten mice were selected as sham group and 36 mice as model group which further assigned into different groups. Relationship between miR-339-3p and Anxa3 was detected by dual luciferase reporter gene assay...
April 25, 2018: Journal of Cellular Biochemistry
Congwen Yang, Keyi Lv, Bin Chen, Yong Yang, Xiangfa Ai, Hongfu Yu, Yihui Yang, Bin Yi, Kaizhi Lu
No abstract text is available yet for this article.
April 12, 2018: Experimental Cell Research
DongDong Chai, Lei Zhang, SiWei Xi, YanYong Cheng, Hong Jiang, Rong Hu
BACKGROUND/AIMS: Nuclear erythroid 2-related factor-2 (Nrf2) is a major stress-response transcription factor that has been implicated in regulating ischemic angiogenesis. We investigated the effects of Nrf2 in regulating revascularization and modulating acute lung injury. METHODS: The expression of Nrf2 and sirtuin1 (Sirt1) was assessed in lung tissue by western blotting and immunofluorescence staining after intestinal ischemia/reperfusion (IIR) in Nrf2-/- and wild-type (WT) mice...
March 29, 2018: Cellular Physiology and Biochemistry
Bo Zhang, Wen Niu, Hai-Ying Dong, Man-Ling Liu, Ying Luo, Zhi-Chao Li
It is well established that hypoxia induces epithelial‑mesenchymal transition in vitro and in vivo. However, the role of hypoxia in endothelial‑mesenchymal transition (EndMT), an important process in the pathogenesis of pulmonary hypertension, is not well‑characterized. The present study demonstrated a significant downregulation of the endothelial marker CD31 and its co‑localization with a mesenchymal marker, α‑smooth muscle actin (α‑SMA), in the intimal layer of small pulmonary arteries of rats exposed to chronic hypoxia...
March 22, 2018: International Journal of Molecular Medicine
Juanjuan Li, Qian Chen, Xinhai He, Azeem Alam, Jiaolin Ning, Bin Yi, Kaizhi Lu, Jianteng Gu
BACKGROUND: Acute lung injury caused by renal ischemia-reperfusion is one of the leading causes of acute kidney injury-related death. Dexmedetomidine, an α2 -adrenergic agonist sedative, has been found to have protective effects against acute kidney injury and remote lung injury. We sought to determine whether dexmedetomidine can exert its anti-apoptotic effects in acute lung injury after acute kidney injury, in addition to its common anti-inflammatory effects, and to determine the underlying mechanisms...
March 23, 2018: Journal of Translational Medicine
Wensheng Liu, Kaizhong Liu, Shu Zhang, Lihong Shan, Jiangfeng Tang
BACKGROUND Acute lung injury (ALI) is a life-threatening complication of sepsis. Tetramethylpyrazine (TMP) has been used in the clinical treatment of vascular diseases. The aim of this study was to investigate the therapeutic effects and possible involved mechanisms on ALI. MATERIAL AND METHODS Cecal ligation and puncture (CLP) was used to establish a sepsis model in rats. TMP at various dosages were administrated to rats using a intragastric method. Animal survival rate was calculated. The lung functions were evaluated by lung weight/dry weight ratio (W/D), PaO2, dynamic compliance (DC), and airway resistance index (ARI)...
February 28, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Z Wu, Z Wang, P Xu, M Zhang, L Cheng, B Gong
BACKGROUND: Little is known about the pathogenesis of acute lung injury (ALI) complicated with acute aortic dissection (AAD). OBJECTIVE: We aim to investigate the roles of macrophages-derived matrix metalloproteinase 9 (MMP9) in the development of ALI complicated with AAD and factors involved in the recruitment of macrophages. METHODS: This study included three parts: (i) Determination of serum MMPs, angiotensin II (AngII) and MCP-1 in patients with AAD complicated with ALI or AAD only, non-ruptured chronic aortic aneurysm patients or healthy volunteers using ELISA method...
2017: Current Molecular Medicine
Congwen Yang, Keyi Lv, Bin Chen, Yong Yang, Xiangfa Ai, Hongfu Yu, Yihui Yang, Bin Yi, Kaizhi Lu
BACKGROUND/AIM: Increasing evidence show microRNAs (miRNAs) are associated with hepatopulmonary syndrome (HPS). The aim of this study was to investigate the role of miR-144 in the angiogenesis of HPS, as well as to identify its underlying mechanism. METHODS: The expression levels of miR-144-3p were assessed in pulmonary micro-vascular endothelial cells (PMVECs), as well as in lung tissues from rats with HPS. We predicted the potential target of miR-144-3p. Tyrosine kinase 2(Tie2) was identified as a target gene of miR144-3p, which has an essential role in the angiogenesis of lung vessel...
April 1, 2018: Experimental Cell Research
Orrette R Wauchope, Michelle M Mitchener, William N Beavers, James J Galligan, Jeannie M Camarillo, William D Sanders, Philip J Kingsley, Ha-Na Shim, Thomas Blackwell, Thong Luong, Mark deCaestecker, Joshua P Fessel, Lawrence J Marnett
Reactive oxygen species (ROS) are formed in mitochondria during electron transport and energy generation. Elevated levels of ROS lead to increased amounts of mitochondrial DNA (mtDNA) damage. We report that levels of M1dG, a major endogenous peroxidation-derived DNA adduct, are 50-100-fold higher in mtDNA than in nuclear DNA in several different human cell lines. Treatment of cells with agents that either increase or decrease mitochondrial superoxide levels leads to increased or decreased levels of M1dG in mtDNA, respectively...
February 9, 2018: Nucleic Acids Research
Li-Sha Li, Yong Yang, Xin-Ling Liu, Chuan-Rao Zhang, Qing-Yan Ye, Wen-Jun Hou, Yan-Hua Zhao, Gao-Peng Xiao, Xin-Nan Li, Yan-Hua Li, Rui Liu
OBJECTIVE: To elucidate the pathogenic role of leukotriene B4 (LTB4) in increased pulmonary microvascular endothelial cell permeability induced by one lung ventilation (OLV) in rabbits. METHODS: Forty-eight healthy Japanese white rabbits were randomly divided into control group (group C), saline pretreatment group (group S), bestatin (a leukotriene A4 hydrolase (LTA4H) inhibitor) plus saline pretreatment group (group B), OLV group (group O), saline pretreatment plus OLV group (group SO) and bestatin plus saline pretreatment with OLV group (group BO)...
November 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Shaohua Dai, Qirong Xu, Sheng Liu, Bentong Yu, Jichun Liu, Jian Tang
This study was conducted to investigate the mechanism of autophagy and its signaling pathways in ischemia/reperfusion injury (IRI). Pulmonary microvascular endothelial cells (PMVECs) were used to construct I/R models. The cells were then treated with autophagy inhibitor 3-MA and infected with adenovirus expressing Beclin 1-shRNA. The expression of CD31, LC3-II, Bcl-2, Bax, LC3-II, Beclin 1, AKT, p-AKT, AMPK and p-AMPK, apoptosis, cell viability and migration ability were determined. Over 95% isolated PMVECs were positive for CD31...
2017: American Journal of Translational Research
Juan Wang, Jing Tan, Yanhong Liu, Linlin Song, Di Li, Xiaoguang Cui
The inhibition of mitogen‑activated protein kinases (MAPKs), including c‑Jun NH2‑terminal protein kinase (JNK), p38 MAPK (p38) and extracellular signal‑regulated protein kinase 1/2 (ERK1/2), have an important effect on lung ischemia‑reperfusion injury (IRI) during lung transplantation (LT). However, the way in which combined MAPK inhibition exerts optimal protective effects on lung IRI remains to be elucidated. Therefore, the present study evaluated the therapeutic efficacy of the inhibition of MAPKs in rat pulmonary microvascular endothelial cells (PMVECs) in an IRI model of LT...
January 2018: Molecular Medicine Reports
Gang Wang, Gengyun Sun
OBJECTIVE: To investigate the role of phosphorylated Moesin (p-Moesin) in the injury of pulmonary microvascular endothelial cells (PMVECs) of rats induced by tumor necrosis factor-α (TNF-α), and to approach the impact of Rac1 signal pathway on Moesin phosphorylation. METHODS: PMVECs of rats were cultured in vitro and passed on to the third generation, and the TNF-α time-effect experiment, dose-effect experiment and Rac1 signaling pathway intervention experiment were performed respectively...
September 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Hua-Song Zhou, Meng Li, Bing-Dong Sui, Lei Wei, Rui Hou, Wen-Sheng Chen, Qiang Li, Sheng-Hui Bi, Jin-Zhou Zhang, Ding-Hua Yi
The endotoxin lipopolysaccharide (LPS)-induced pulmonary endothelial barrier disruption is a key pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). However, the molecular mechanisms underlying LPS-impaired permeability of pulmonary microvascular endothelial cells (PMVECs) are not fully understood. Gap junctions, particularly Connexin40 (Cx40), are necessary for the maintenance of normal vascular function. In this study, we for the first time investigated the role of Cx40 in LPS-impaired permeability of PMVECs and provided potential therapeutic approaches based on mechanistic findings of Cx40 regulation by LPS stimuli...
January 2018: Microvascular Research
Aron B Fisher, Jose P Vasquez-Medina, Chandra Dodia, Elena M Sorokina, Jian-Qin Tao, Sheldon I Feinstein
Although lipid peroxidation associated with oxidative stress can result in cellular death, sub-lethal lipid peroxidation can gradually resolve with return to the pre-exposure state. We have shown that resolution of lipid peroxidation is greatly delayed in lungs or cells that are null for peroxiredoxin 6 (Prdx6) and that both the phospholipase A2 and the GSH peroxidase activities of Prdx6 are required for a maximal rate of recovery. Like other peroxiredoxins, Prdx6 can reduce H2 O2 and short chain hydroperoxides, but in addition can directly reduce phospholipid hydroperoxides...
April 2018: Redox Biology
Ming-Ju Xu, Bao-Jian Liu, Cun-Lian Wang, Guo-Hua Wang, Yong Tian, Shao-Hua Wang, Jun Li, Pei-Yao Li, Rui-Hua Zhang, Dong Wei, Shu-Fei Tian, Tong Xu
Epigallocatechin-3-gallate (EGCG) was found to inhibit the Toll-like receptor 4 (TLR4) pathway involved in influenza virus pathogenesis. Here, the effect of EGCG on TLR4 in an H9N2 virus-induced acute lung injury mouse model was investigated. BALB/c mice were inoculated intranasally with A/Swine/Hebei/108/2002 (H9N2) virus or noninfectious allantoic fluid, and treated with EGCG and E5564 or normal saline orally for 5 consecutive days. PMVECs were treated with EGCG or anti-67kDa laminin receptor (LR). Lung physiopathology, inflammation, oxidative stress, viral replication, and TLR4/NF-κB/Toll-interacting protein (Tollip) pathway in lung tissue and/or PMVECs were investigated...
November 2017: International Immunopharmacology
Wei Ren, Zhiwei Wang, Zhiyong Wu, Zhipeng Hu, Feifeng Dai, Jinxing Chang, Bowen Li, Huagang Liu, Yongle Ruan
Patients with aortic dissection (AD) may present acute lung injury (ALI) that may affect the prognosis. In this study, we aim to investigate the roles and mechanism of IL-22 in the pathogenesis of AD complicated with ALI. Six hundred and twenty-one AD patients were included, and the incidence of ALI and pulmonary CT findings were analyzed. Mouse ALI model was established through AngII, and then IL-22 injection and AG490 were given. The pathological changes, infiltration of inflammatory cells, and expression of STAT3 were determined...
2017: Disease Markers
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