Bingyan Zhou, Chenzhao Feng, Song Sun, Xuyong Chen, Didi Zhuansun, Di Wang, Xiaosi Yu, Xinyao Meng, Jun Xiao, Luyao Wu, Jing Wang, Jing Wang, Ke Chen, Zejian Li, Jingyi You, Handan Mao, Shimin Yang, Jiaxin Zhang, Chunlei Jiao, Zhi Li, Donghai Yu, Xiaojuan Wu, Tianqi Zhu, Jixin Yang, Lei Xiang, Jiazhe Liu, Tailiang Chai, Juan Shen, Chuan-Xi Mao, Juncheng Hu, Xingjie Hao, Bo Xiong, Shan Zheng, Zhihua Liu, Jiexiong Feng
During enteric nervous system (ENS) development, pioneering wavefront enteric neural crest cells (ENCCs) initiate gut colonization. However, the molecular mechanisms guiding their specification and niche interaction are not fully understood. We used single-cell RNA sequencing and spatial transcriptomics to map the spatiotemporal dynamics and molecular landscape of wavefront ENCCs in mouse embryos. Our analysis shows a progressive decline in wavefront ENCC potency during migration and identifies transcription factors governing their specification and differentiation...
April 15, 2024: Developmental Cell