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Coagulase-negative staphylococci

Ayse Ruveyda Ugur, Hatice Turk Dagi, Bahadir Ozturk, Gulsum Tekin, Duygu Findik
BACKGROUND: Methicillin resistance is a serious health concern since it has spread among Staphylococcus aureus and coagulase-negative Staphylococci (CoNS) that are frequent community and nosocomial pathogens worldwide. Methicillin-resistant strains are often resistant to other classes of antibiotics, making their treatment difficult. Nigella sativa oil is known to be active against Gram-positive cocci, yet its in vitro cytotoxicity is rarely investigated, is a proper and powerful candidate for treatment of methicillin-resistant isolates...
July 2016: Pharmacognosy Magazine
T Banach, M Bochniarz, P Łyp, Ł Adaszek, W Wawron, B Furmaga, M Skrzypczak, J Ziętek, S Winiarczyk
The aim of this study was to use matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of coagulase-negative staphylococci (CNS) isolated from the milk of cows with subclinical mastitis. The study material consisted of 33 isolates of CNS, identified by the results of API Staph tests, obtained from the milk of cows with subclinical mastitis. Based on the spectra analyses, MALDI-TOF MS tests of 33 bacterial samples allowed identification of the microorganisms in 27 cases (81...
September 1, 2016: Polish Journal of Veterinary Sciences
I Klimiene, M Virgailis, A Pavilonis, R Siugzdiniene, R Mockeliunas, M Ruzauskas
The objectives of this study were to determine the prevalence and antimicrobial resistance of coagulase-negative staphylococci (CNS) isolated from dairy cows with subclinical mastitis. Antimicrobial resistance in staphylococci were evaluated by breakpoint values specific to the species (EU-CAST). The presence of resistance-encoding genes was detected by multiplex PCR. A total of 191 CNS isolates were obtained. The CNS isolates were typically resistant to penicillin (67.4%), tetracyc-line (18.9%), and erythromycin (13...
September 1, 2016: Polish Journal of Veterinary Sciences
M Palaniappan, Padmini V Usha, P Balamurugan, Sree P Sanbaka, B Vetriveeran, Hrudya Venugopal, S Rajesh, M Gayathri, M Raveendran
We report a case of isolated native tricuspid valve infective endocarditis caused by Coagulase Negative Staphylococcus in a non-IV drug abuser without structural heart disease. Early diagnosis and prompt initiation of appropriate treatment saved the patient without surgical intervention. The recent trend of Coagulase Negative Staphylococci (CoNS) causing native valve endocarditis (NVE) is reviewed with literature.
July 2016: Journal of the Association of Physicians of India
H Humphreys, P Jenks, J Wilson, V Weston, R Bayston, C Waterhouse, A Moore
BACKGROUND: The insertion of external ventricular drains (EVDs) is necessary in some neurosurgical patients, but increases the risk of meningitis/ventriculitis. While there are well-recognized risk factors, the proportion of patients who develop meningitis/ventriculitis varies partly due to differences in definitions. A multi-disciplinary working group was established to agree definitions for EVD-associated meningitis/ventriculitis, and a surveillance system was piloted in four centres in the UK and Ireland...
September 17, 2016: Journal of Hospital Infection
Caroline Rouard, Elisabeth Aslangul, Alexandre Rivière, Claire Deback, Marie-José Butel, Florence Doucet-Populaire, Nadège Bourgeois-Nicolaos
Linezolid (LZD) has arisen as an alternative treatment in diabetic foot osteitis due to staphylococci. LZD resistance selection is difficult and involved various molecular mechanisms. As a better knowledge of those mechanisms could be beneficial for pathogenic strains' screening, we simulated in vitro the spontaneous mutagenesis process that leads to LZD-resistant strains from two Staphylococcus epidermidis strains responsible for monomicrobial diabetic foot osteitis. LZD high resistance was selected for both strains, with the same timeline of mutation appearance...
October 18, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Maria Lennerås, Georgios Tsikandylakis, Margarita Trobos, Omar Omar, Forugh Vazirisani, Anders Palmquist, Örjan Berlin, Rickard Brånemark, Peter Thomsen
The breach of the skin barrier is a critical issue associated with the treatment of individuals with transfemoral amputation (TFA) using osseointegrated, percutaneous titanium implants. Thirty TFA patients scheduled for abutment exchange or removal were consecutively enrolled. The aims were to determine the macroscopic skin signs, the presence of bacteria and the gene expression in abutment-adherent cells and to conduct correlative and comparative analyses between the different parameters. Redness and a granulation ring were present in 47% of the patients...
October 17, 2016: Journal of Biomedical Materials Research. Part A
Anaïs L Carniciu, Jonathan Chou, Ilya Leskov, Suzanne K Freitag
PURPOSE: This study retrospectively reviews preseptal cellulitis and abscesses involving the eyebrow to elucidate the bacteriology and potential causative factors. METHODS: A retrospective chart review was conducted to identify patients who had been diagnosed with preseptal cellulitis or abscess involving the eyebrow at the Massachusetts Eye and Ear Infirmary between 2008 and 2015. Demographic, clinical, and microbiological data were collected. RESULTS: Eighty patients with eyebrow infections were identified, of whom 49 (61...
October 5, 2016: Ophthalmic Plastic and Reconstructive Surgery
Santiago Pérez-Parra, Alejandro Peña-Monje, Juan Luis Recio, Federico García-García
No abstract text is available yet for this article.
October 12, 2016: Enfermedades Infecciosas y Microbiología Clínica
Sheryl A Zelenitsky, Jacy Howarth, Philippe Lagace-Wiens, Christie Sathianathan, Robert Ariano, Christine Davis, Mauro Verrelli
♦ Background: Information related to the microbiology of peritonitis is critical to the optimal management of patients receiving peritoneal dialysis (PD). The goal was to characterize the microbiological etiology and antimicrobial susceptibilities of PD-related peritonitis (PDRP) from 2005 to 2014, inclusive. ♦ Methods: The distribution of organisms in culture-positive PDRP was described for new episodes and relapse infections, and further detailed for monomicrobial and polymicrobial peritonitis. Annual and overall rates of PDRP were also characterized...
October 13, 2016: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
S H S P Pedroso, S H C Sandes, K C M Luiz, R S Dias, R A T Filho, J C Serufo, L M Farias, M A R Carvalho, M R Q Bomfim, S G Santos
Coagulase-negative staphylococci (CNS) represent one of the most prevalent microorganisms in nosocomial infections worldwide, nevertheless little is known about their pathogenicity features. Thus, our aim was to characterize virulence aspects of CNS isolated from patients with bloodstream infections assisted in hospitals of Belo Horizonte, MG, Brazil. Strains were identified using bioMérieuxVitek(®) and for biofilm production evaluation, Congo Red Agar (CRA) and polystyrene plates were used. PCR was applied to detect icaA, icaB, icaC, atlE, sea, sec, sed, tsst-1 and agr...
October 7, 2016: Microbial Pathogenesis
Vijaya Shivanna, S R Sunkappa, D Venkatesha
Neonatal septicemia is one of the leading causes of neonatal mortality and morbidity worldwide. Hence, the present study was undertaken to isolate the bacteria causing neonatal sepsis and determine their antibiotic susceptibility pattern. Fifty neonates suspected to have septicemia were screened for 2 months (July and August 2014). Out of 50 specimen, 15 (30%) were blood culture positive. Coagulase-negative staphylococci was the most common isolate (10, 66.6%), with 60% (6 isolates) methicillin resistance. In view of the increasing antibiotic resistance, periodic surveillance should be conducted to control the emergence and spread of antimicrobial resistance...
October 2016: Indian Journal of Pathology & Microbiology
B D Enger, R R White, S C Nickerson, L K Fox
Two meta-analyses were conducted using data from peer-reviewed natural exposure (NE) and experimental challenge (EC) teat dip efficacy trials to identify factors influencing the new intramammary infection (IMI) rate. A NE data set containing 16 studies and an EC data set containing 21 studies were created. New IMI rate was calculated based on the percentage of new quarter infections per month (PNQI/mo) for each observation, in both data sets, and used as the dependent variable for model derivation. A linear, mixed-effects model with a random study effect, weighted by number of quarters eligible for infection, was derived for each data set...
October 5, 2016: Journal of Dairy Science
Yasushi Matsuda, Hideki Mitomo, Tetsu Sado, Masafumi Noda, Miki Akiba, Yoshinori Okada
Lung transplantation is getting to be a common treatment for the end-stage respiratory failure. Therefore we might encounter the various issues associated with infection under immunosuppression. At 1st, virus infections occur by Cytomegalovirus, Epstein-Barr virus, hepatitis B and C virus and John Cunningham virus. The 2nd, bacterial infections exacerbate graft condition by methicillin-resistant Staphylococcus aureus, Pseudomonas, Burkholderia cepacia and coagulase negative staphylococci. The 3rd, fungal infections are induced by Aspergillus, Candida and Cryptococcus...
October 2016: Kyobu Geka. the Japanese Journal of Thoracic Surgery
Thuy Doan, Lakshmi Akileswaran, Dallin Andersen, Benjamin Johnson, Narae Ko, Angira Shrestha, Valery Shestopalov, Cecilia S Lee, Aaron Y Lee, Russell N Van Gelder
Purpose: To characterize the ocular surface microbiome of healthy volunteers using a combination of microbial culture and high-throughput DNA sequencing techniques. Methods: Conjunctival swab samples from 107 healthy volunteers were analyzed by bacterial culture, 16S rDNA gene deep sequencing (n = 89), and biome representational in silico karyotyping (BRiSK; n = 80). Swab samples of the facial skin (n = 42), buccal mucosa (n = 50), and environmental controls (n = 27) were processed in parallel...
October 1, 2016: Investigative Ophthalmology & Visual Science
Vincenzo Papa, Anna Rita Blanco, Marcello Santocono
PURPOSE: To characterize the ocular flora in a consecutive group of patients having cataract surgery and to determine the antibiotic susceptibility profile of isolates to several ophthalmic antibiotics. SETTING: Hospital Di Stefano, Catania, Italy. DESIGN: Observational case series. METHODS: Conjunctival and eyelid cultures from patients were obtained 14 days before surgery and, if positive, repeated the day of the surgery...
September 2016: Journal of Cataract and Refractive Surgery
Myrella Cariry Lira, Paricia Emília Naves Givisiez, Francisca Geovânia Canafístula De Sousa, Marciane Magnani, Evandro Leite De Souza, Denis Augusto Spricigo, Wondwossen Abebe Gebreyes, Celso José Bruno De Oliveira
INTRODUCTION: Biofilm-associated antimicrobial resistance is of increasing importance to the maintenance and spread of foodborne pathogens in the food industry. This study aimed to investigate the ability to form biofilm and the antimicrobial resistance of staphylococci contaminating small-scale goat milk dairy plants. METHODOLOGY: Sixty isolates were tested for antimicrobial resistance against 20 drugs by the microdilution method. Biofilm-forming traits were assessed by the microtiter plate method (MtP), Congo red agar method (CRA), and icaD gene detection by polymerase chain reaction (PCR)...
September 30, 2016: Journal of Infection in Developing Countries
Dragana Protic, Dragana Savic, Dragana Andjelkovic, Nina Djukanovic, Marija Zdravkovic, Sinisa F Djurasevic, Zoran Todorovic
INTRODUCTION: Coagulase-negative staphylococci (CoNS) are increasingly resistant nosocomial pathogens. We aimed to analyze the prevalence of CoNS isolates in clinical settings, the evolution of antimicrobial resistance of CoNS, and antibiotic consumption in a hospital. METHODOLOGY: This retrospective cohort study was carried out at a tertiary healthcare facility over 17 months. Identification of isolated cultures and antibiotic susceptibility testing were performed using the Vitek2 system...
September 30, 2016: Journal of Infection in Developing Countries
M Bochniarz, Ł Adaszek, B Dzięgiel, A Nowaczek, W Wawron, R Dąbrowski, M Szczubiał, S Winiarczyk
The aim of this study was to recognize selected factors of virulence determining the adhesion of Staphylococcus chromogenes to cows' udder tissues in subclinical mastitis and to evaluate the susceptibility of this pathogen to antibiotics. The subjects of the study were 38 isolates of Staph. chromogenes from 335 samples of milk from cows with subclinical coagulase-negative staphylococci mastitis. Somatic cell count ranged between 216,000 and 568,000/mL of milk (average 356,000/mL of milk). We confirmed the ability to produce slime in 24 isolates (63...
September 28, 2016: Journal of Dairy Science
Alison Harvey, Isabelle Kilcoyne, Barbara A Byrne, Jorge Nieto
OBJECTIVE: To compare synovial concentrations of amikacin following intravenous regional limb perfusion (IVRLP) with two different doses, and to compare their ability to reach target concentrations for bacterial isolates from common orthopedic conditions. STUDY DESIGN: Randomized crossover experiment. ANIMALS: Six adult horses. METHODS: Horses received IVRLP with 2 and 3 g of amikacin in the cephalic vein of alternate limbs (20 minutes tourniquet application and ≥14 days washout period)...
September 29, 2016: Veterinary Surgery: VS
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