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https://www.readbyqxmd.com/read/27902410/inhibition-of-quorum-sensing-controlled-virulence-factors-of-pseudomonas-aeruginosa-by-murraya-koenigii-essential-oil-a-study-in-a-caenorhabditis-elegans-infectious-model
#1
P Sankar Ganesh, Ravishankar Vittal Rai
The global emergence of antibiotic resistant strains of Pseudomonas aeruginosa poses a major threat in hospital environments as well as in the community. P. aeruginosa is an opportunistic human pathogen and it also infects a wide range of model organisms including the nematode Caenorhabditis elegans. Quorum sensing (QS) mediates cell-to-cell communication in bacteria and has an important role in regulating virulence genes, antibiotic resistance and biofilm formation which are crucial for establishment of the infection...
November 3, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27900439/non-ribosomal-peptide-synthases-from-pseudomonas-aeruginosa-play-a-role-in-cyclodipeptide-biosynthesis-quorum-sensing-regulation-and-root-development-in-a-plant-host
#2
Omar González, Randy Ortíz-Castro, César Díaz-Pérez, Alma L Díaz-Pérez, Viridiana Magaña-Dueñas, José López-Bucio, Jesús Campos-García
Diverse molecules mediate cross-kingdom communication between bacteria and their eukaryotic partners and determine pathogenic or symbiotic relationships. N-acyl-L-homoserine lactone-dependent quorum-sensing signaling represses the biosynthesis of bacterial cyclodipeptides (CDPs) that act as auxin signal mimics in the host plant Arabidopsis thaliana. In this work, we performed bioinformatics, biochemical, and plant growth analyses to identify non-ribosomal peptide synthase (NRPS) proteins of Pseudomonas aeruginosa, which are involved in CDP synthesis...
November 30, 2016: Microbial Ecology
https://www.readbyqxmd.com/read/27874198/crataeva-nurvala-nanoparticles-inhibit-virulence-factors-and-biofilm-formation-in-clinical-isolates-of-pseudomonas-aeruginosa
#3
Syed Ghazanfar Ali, Mohammad Azam Ansari, Haris M Khan, Mohammad Jalal, Abbas Ali Mahdi, Swaranjit Singh Cameotra
Green synthesized nanoparticles have gained great attention due to their non-toxic and non-hazardous nature. In the present study, bark extract of the medicinal plant in Ayurveda Crataeva nurvala (Buch-Ham) (CN) was chosen for the biosynthesis of silver nanoparticles (AgNPs). These NPs were characterized by Ultra violet visible spectroscopy, Fourier Transform Infra Red, Atomic Force Microscopy, and Transmission Electron Microscopy (TEM). The average particle size of green synthesized CN-AgNPs was 15.2 ± 1...
November 22, 2016: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/27860087/the-effect-of-burdock-leaf-fraction-on-adhesion-biofilm-formation-quorum-sensing-and-virulence-factors-of-p-aeruginosa
#4
Zaixiang Lou, Hongxin Wang, Yuxia Tang
AIMS: This study aimed to evaluate the effect of a fraction of burdock (Arctium lappa L.) leaf on the initial adhesion, biofilm formation, quorum sensing and virulence factors of P. aeruginosa. METHODS AND RESULTS: Anti-biofilm activity of the burdock leaf fraction was studied by the method of crystal violet staining. When the concentration of the burdock leaf fraction was 2.0 mg mL(-1) , the inhibition rates on biofilm formation of P. aeruginosa were 100%. The burdock leaf fraction was found to inhibit the formation of biofilm by reducing bacterial surface hydrophobicity, decreasing bacterial aggregation ability and inhibiting swarming motility Interestingly, the burdock leaf fraction inhibited the secretion of quorum sensing (QS) signaling molecule 3-oxo-C12-HSL and interfered quorum sensing...
November 12, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27834332/attenuation-of-quorum-sensing-regulated-behaviour-by-tinospora-cordifolia-extract-identification-of-its-active-constituents
#5
Viraj C Gala, Nithya R John, Ashok M Bhagwat, Ajit G Datar, Prashant S Kharkar, Krutika B Desai
BACKGROUND & OBJECTIVES: The pathogenicity of the nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii is regulated by their quorum sensing (QS) systems. The objective of the present study was to examine the effect of the cold ethyl acetate extract of Tinospora cordifolia stem on virulence and biofilm development in the wild type and clinical strains of P. aeruginosa and A. baumannii. The study was further aimed to identify the probable active constituents in the plant extract...
July 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/27784787/epigallocatechin-gallate-remodels-overexpressed-functional-amyloids-in-pseudomonas-aeruginosa-and-increases-biofilm-susceptibility-to-antibiotic-treatment
#6
Marcel Stenvang, Morten S Dueholm, Brian S Vad, Thomas W Seviour, Guanghong Zeng, Susana Geifman-Shochat, Mads T Søndergaard, Gunna Christiansen, Rikke Louise Meyer, Staffan Kjelleberg, Per Halkjær Nielsen, Daniel E Otzen
Epigallocatechin-3-gallate (EGCG) is the major polyphenol in green tea. It has anti-microbial properties and disrupts the ordered structure of amyloid fibrils involved in human disease. The anti-microbial effect of EGCG against the opportunistic pathogen Pseudomonas aeruginosa has been shown to involve disruption of quorum sensing (QS). Functional amyloid fibrils in P. aeruginosa (Fap) are able to bind and retain quorum sensing molecules, suggesting that EGCG interferes with QS through structural remodeling of amyloid fibrils...
October 26, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27763824/blue-light-treatment-of-pseudomonas-aeruginosa-strong-bactericidal-activity-synergism-with-antibiotics-and-inactivation-of-virulence-factors
#7
Grzegorz Fila, Anna Kawiak, Mariusz Grinholc
Pseudomonas aeruginosa is among the most common pathogens responsible for both acute and chronic infections of high incidence and severity. Additionally, P. aeruginosa resistance to conventional antimicrobials has increased rapidly over the past decade. Therefore, it is crucial to explore new therapeutic options, particularly options that specifically target the pathogenic mechanisms of this microbe. The ability of a pathogenic bacterium to cause disease is dependent upon the production of agents termed 'virulence factors', and approaches to mitigate these agents have gained increasing attention as new antibacterial strategies...
October 20, 2016: Virulence
https://www.readbyqxmd.com/read/27761766/de-novo-synthesis-and-functional-analysis-of-polyphosphate-loaded-poly-ethylene-glycol-hydrogel-nanoparticles-targeting-pyocyanin-and-pyoverdin-production-in-pseudomonas-aeruginosa-as-a-model-intestinal-pathogen
#8
Yushu Yin, Georgia Papavasiliou, Olga Y Zaborina, John C Alverdy, Fouad Teymour
The human gastrointestinal tract is the primary site of colonization of multidrug resistant pathogens and the major source of life-threatening complications in critically ill and immunocompromised patients. Eradication measures using antibiotics carry further risk of antibiotic resistance. Furthermore, antibiotic treatment can adversely shift the intestinal microbiome toward domination by resistant pathogens. Therefore, approaches directed to prevent replacement of health promoting microbiota with resistant pathogens should be developed...
October 19, 2016: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/27725812/transcriptomic-and-metabolomic-analysis-revealed-multifaceted-effects-of-phage-protein-gp70-1-on-pseudomonas-aeruginosa
#9
Xia Zhao, Canhuang Chen, Xingyu Jiang, Wei Shen, Guangtao Huang, Shuai Le, Shuguang Lu, Lingyun Zou, Qingshan Ni, Ming Li, Yan Zhao, Jing Wang, Xiancai Rao, Fuquan Hu, Yinling Tan
The impact of phage infection on the host cell is severe. In order to take over the cellular machinery, some phage proteins were produced to shut off the host biosynthesis early in the phage infection. The discovery and identification of these phage-derived inhibitors have a significant prospect of application in antibacterial treatment. This work presented a phage protein, gp70.1, with non-specific inhibitory effects on Pseudomonas aeruginosa and Escherichia coli. Gp70.1 was encoded by early gene - orf 70...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27713984/heracleum-orphanidis-chemical-characterisation-and-comparative-evaluation-of-antioxidant-and-antimicrobial-activities-with-specific-interest-in-the-influence-on-pseudomonas-aeruginosa-pao1
#10
Ksenija S Mileski, Ana D Ćirić, Snežana S Trifunović, Mihailo S Ristić, Marina D Soković, Vlado S Matevski, Vele V Tešević, Milka B Jadranin, Petar D Marin, Ana M Džamić
GC-FID and GC-MS were used to determine the chemical composition of the essential oil of the aerial parts of Heracleum orphanidis Boiss. Also, the HPLC-DAD/ESI-ToF-MS profile of the methanol extracts of aerial parts and roots was determined. The main components of the essential oil were n-octanol, octyl hexanoate and n-octyl acetate, while coumarins were the most prevalent compounds in methanol extracts. An evaluation of antioxidant activity showed that the methanol and aqueous extracts of the aerial parts had the highest potential...
September 14, 2016: Food & Function
https://www.readbyqxmd.com/read/27696655/coprinuslactone-protects-the-edible-mushroom-coprinus-comatus-against-biofilm-infections-by-blocking-both-quorum-sensing-and-mura
#11
Maira P de Carvalho, Giuseppe Gulotta, Matheus W do Amaral, Heinrich Lünsdorf, Florenz Sasse, Wolf-Rainer Abraham
Pathogens embedded in biofilms are involved in many infections and are very difficult to treat with antibiotics because of higher resistance compared with planktonic cells. Therefore, new approaches for their control are urgently needed. One way to search for biofilm dispersing compounds is to look at defense strategies of organisms exposed to wet environments, which makes them prone to biofilm infections. It is reasonable to assume that mushrooms have developed mechanisms to control biofilms on their sporocarps (fruiting bodies)...
November 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27671284/pyocyanin-as-anti-tyrosinase-and-anti-tinea-corporis-a-novel-treatment-study
#12
REVIEW
Nessma A El-Zawawy, Sameh S Ali
OBJECTIVE: The aim of this study was to evaluate the efficiency of pyocyanin pigment as a novel compound active against tyrosinase with its depigmentation efficiency for combating Trichophyton rubrum which could be a major causative agent of tinea corporis. METHODS: Fifty swabs of fungal tinea corporis infections were collected and identified. Five MDRPA isolates were tested for their levels of pyocyanin production. The purified extracted pyocyanin was characterized by UV spectrum and FT-IR analysis...
November 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27664726/role-of-ppgpp-in-pseudomonas-aeruginosa-acute-pulmonary-infection-and-virulence-regulation
#13
Xiaohui Xu, Hua Yu, Di Zhang, Junzhi Xiong, Jing Qiu, Rong Xin, Xiaomei He, Halei Sheng, Wenqiang Cai, Lu Jiang, Kebin Zhang, Xiaomei Hu
During infection, bacteria might generate adaptive responses to facilitate their survival and colonization in the host environment. The alarmone guanosine 5'-triphosphate-3'-diphosphate (ppGpp), the levels of which are regulated by the RelA and SpoT enzymes, plays a critical role in mediating bacterial adaptive responses and virulence. However, the mechanism by which ppGpp regulates virulence-associated traits in Pseudomonas aeruginosa is poorly understood. To investigate the regulatory role of ppGpp, the ppGpp-deficient strain ΔRS (relA and spoT gene double mutant) and the complemented strain ΔRS(++) (complemented with relA and spoT genes) were constructed...
November 2016: Microbiological Research
https://www.readbyqxmd.com/read/27645245/potential-use-of-dimethyl-sulfoxide-in-treatment-of-infections-caused-by-pseudomonas-aeruginosa
#14
Qiao Guo, Qiaolian Wu, Dangdang Bai, Yang Liu, Lin Chen, Sheng Jin, Yuting Wu, Kangmin Duan
Dimethyl Sulfoxide (DMSO) is commonly used as a solvent to dissolve water-insoluble drugs or other test samples in both in vivo and in vitro experiments. It was observed during our experiment that DMSO at non-inhibitory concentrations could significantly inhibit pyocyanin production in human pathogen Pseudomonas aeruginosa. Pyocyanin is an important pathogenic factor whose production is controlled by cell-density dependent quorum-sensing (QS) system. Investigation of the effect of DMSO on QS shows that DMSO has significant QS antagonistic activities, and micromolar range concentrations of DMSO attenuated a battery of QS-controlled virulence factors, including rhamnolipids, elastase, LasA protease and biofilm formation...
September 19, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27634359/phytochemicals-as-effective-quorum-quenchers-against-bacterial-communication
#15
Subramaniyan S, Divyasree S, Sandhia G S
BACKGROUND: Quorum sensing or the bacterial information flow in an orchestrated manner is an essential feature of many pathogenic bacteria. Quorum quenching molecules (QQ) can inhibit the growth of such bacteria. OBJECTIVE: The purpose of this study is to evaluate the potential of plant extracts as quorum quenchers and monitor the recent patents. METHODS: Many available reports and patents are on synthetic ligand molecules or even compounds isolated from cyanobacteria (Honaucin A) and other microorganisms inhibiting quorum sensing molecules...
September 8, 2016: Recent Patents on Biotechnology
https://www.readbyqxmd.com/read/27613716/transient-proteotoxicity-of-bacterial-virulence-factor-pyocyanin-in-renal-tubular-epithelial-cells-induces-er-related-vacuolation-and-can-be-efficiently-modulated-by-iron-chelators
#16
Valeri V Mossine, James K Waters, Deborah L Chance, Thomas P Mawhinney
Persistent infections of biofilm forming bacteria, such as Pseudomonas aeruginosa, are common among human populations, due to the bacterial resistance to antibiotics and other adaptation strategies, including release of cytotoxic virulent factors such as pigment pyocyanin (PCN). Urinary tract infections harbor P. aeruginosa strains characterized by the highest PCN-producing capacity, yet no information is available on PCN cytotoxicity mechanism in kidney. We report here that renal tubular epithelial cell (RTEC) line NRK-52E responds to PCN treatments with paraptosis-like activity features...
September 9, 2016: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/27559393/discovery-of-an-inhibitor-of-the-production-of-the-pseudomonas-aeruginosa-virulence-factor-pyocyanin-in-wild-type-cells
#17
Bernardas Morkunas, Balint Gal, Warren R J D Galloway, James T Hodgkinson, Brett M Ibbeson, Yaw Sing Tan, Martin Welch, David R Spring
Pyocyanin is a small molecule produced by Pseudomonas aeruginosa that plays a crucial role in the pathogenesis of infections by this notorious opportunistic pathogen. The inhibition of pyocyanin production has been identified as an attractive antivirulence strategy for the treatment of P. aeruginosa infections. Herein, we report the discovery of an inhibitor of pyocyanin production in cultures of wild-type P. aeruginosa which is based around a 4-alkylquinolin-2(1H)-one scaffold. To the best of our knowledge, this is the first reported example of pyocyanin inhibition by a compound based around this molecular framework...
2016: Beilstein Journal of Organic Chemistry
https://www.readbyqxmd.com/read/27539815/an-insight-into-anti-biofilm-and-anti-quorum-sensing-activities-of-the-selected-anthocyanidins-the-case-study-of-pseudomonas-aeruginosa-pao1
#18
Boris Pejin, Ana Ciric, Jasmina Dimitric Markovic, Jasmina Glamoclija, Milos Nikolic, Marina Sokovic
Anti-biofilm activity of three anthocyanidins (pelargonidin, cyanidin and delphinidin) was evaluated for the first time at in vitro conditions. All the compounds reduced the formation of Pseudomonas aeruginosa PAO1 biofilm at low sub-MIC (0.125 MIC) with delphinidin (c 56.25 μg/mL) being the most active (43%). In comparison, ampicillin (c 93.75 μg/mL) and streptomycin (c 21.25 μg/mL) (used as positive controls) were considerably less effective at the same sub-MIC (8 and 12%, respectively). Furthermore, at 0...
August 19, 2016: Natural Product Research
https://www.readbyqxmd.com/read/27517959/cellular-effects-of-pyocyanin-a-secreted-virulence-factor-of-pseudomonas-aeruginosa
#19
REVIEW
Susan Hall, Catherine McDermott, Shailendra Anoopkumar-Dukie, Amelia J McFarland, Amanda Forbes, Anthony V Perkins, Andrew K Davey, Russ Chess-Williams, Milton J Kiefel, Devinder Arora, Gary D Grant
Pyocyanin has recently emerged as an important virulence factor produced by Pseudomonas aeruginosa. The redox-active tricyclic zwitterion has been shown to have a number of potential effects on various organ systems in vitro, including the respiratory, cardiovascular, urological, and central nervous systems. It has been shown that a large number of the effects to these systems are via the formation of reactive oxygen species. The limitations of studies are, to date, focused on the localized effect of the release of pyocyanin (PCN)...
2016: Toxins
https://www.readbyqxmd.com/read/27464037/electrochemical-sensing-of-biomarker-for-diagnostics-of-bacteria-specific-infections
#20
Fatima AlZahra'a Alatraktchi, Helle Krogh Johansen, Søren Molin, Winnie Edith Svendsen
AIM: Pseudomonas aeruginosa is a pathogen that is prevalent in serious infections in compromised patients worldwide. A unique virulence factor of this bacterium is the redox-active molecule pyocyanin, which is a potential biomarker for the identification of P. aeruginosa infections. Here we report a direct, selective and rapid detection technique of pyocyanin. MATERIALS & METHODS: Pyocyanin was detected by amperometry at a relatively high potential where the pyocyanin signal was unaffected by background contributions...
August 2016: Nanomedicine
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