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https://www.readbyqxmd.com/read/28904418/leaf-extracts-of-selected-gardening-trees-can-attenuate-quorum-sensing-and-pathogenicity-of-pseudomonas-aeruginosa-pao1
#1
Kaimin Niu, Min Kuk, Haein Jung, Kokgan Chan, Sooki Kim
An increasing concern on resistance to multiple-antibiotics has led to the discovery of novel agents and the establishment of new precaution strategy. Numerous plant sources have been widely studied to reduce virulence of pathogenic bacteria by interfering cell-to-cell based communication called quorum sensing (QS). Leaf extracts of 17 gardening trees were collected and investigated for their anti-QS effects using a sensor strain Chromobacterium violaceum CV026. Methanolic extracts of K4 (Acer palmatum), K9 (Acer pseudosieboldianum) and K13 (Cercis chinensis) leaves were selected for further experiments based on their antagonism effect on QS without inhibiting C...
September 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28878346/impact-of-phenolic-compounds-in-the-acyl-homoserine-lactone-mediated-quorum-sensing-regulatory-pathways
#2
Md Akil Hossain, Seung-Jin Lee, Na-Hye Park, Abraham Fikru Mechesso, Biruk Tesfaye Birhanu, JeongWoo Kang, Md Ahsanur Reza, Joo-Won Suh, Seung-Chun Park
Quorum sensing (QS) is a cell density-dependent regulation of virulent bacterial gene expression by autoinducers that potentially pertains in the epidemic of bacterial virulence. This study was initially designed to evaluate the effect of 5 phenolic compounds in the modulation of QS and virulence factors of Chromobacterium violaceum and Pseudomonas aeruginosa, and to determine the mechanisms of their effects. Biosensor strains were used to assess antibacterial and anti-QS effect of these compounds. Only methyl gallate (MG) among these compounds demonstrated profound anti-QS effect in the preliminary study, and thus only MG was utilized further to evaluate the effects on the synthesis and activity of acyl homoserine lactone (AHL) in C...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28867635/effect-of-human-placental-extract-in-the-management-of-biofilm-mediated-drug-resistance-a-focus-on-wound-management
#3
Sutapa Goswami, Ratul Sarkar, Pritam Saha, Amit Maity, Tridib Sarkar, Debmalya Das, Piyali Datta Chakraborty, Subhasri Bandyopadhyay, Chandan Kumar Ghosh, Sanmoy Karmakar, Tuhinadri Sen
Management of infectious wounds, particularly chronic wounds and burn injuries, is a matter of global concern. Worldwide estimates reveal that, billions of dollars are being spent annually for the management of such chronic ailments. Evidently, bacterial biofilms pose a greater problem in the effective management of infection in chronic wounds, since most of the currently available antibiotics are unable to act on the microorganisms residing inside the protected environment of the biofilms. Accordingly, in the present study, we have attempted to evaluate the anti-biofilm properties of human placental extract (PLX) and also other virulence factors that are mediated via the quorum sensing (QS) signalling system...
September 1, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28860065/chitosan-extracted-from-aspergillus-flavus-shows-synergistic-effect-eases-quorum-sensing-mediated-virulence-factors-and-biofilm-against-nosocomial-pathogen-pseudomonas-aeruginosa
#4
Sahira Nsayef Muslim, Israa M S Al Kadmy, Alaa Naseer Mohammed Ali, Batool Kadham Salman, Mohammad Ahmad, Saba Saadoon Khazaal, Nadheema Hammood Hussein, Sraa Nsayef Muslim
The biological methods for extraction of chitosan were used as alternative procedures for chemical methods In biological methods, the chitosan was extracted from A. flavus by using of Lactobacillus paracasei for demineralization and Bacillus subtilis for deproteinization. The yield of extracted chitosan reached to 53.8%, pH was 7.8 and complete solubility in 1% acitic acid. Purified chitosan had the ability to reduce the biofilm forming capacity of P. aeruginosa clearly visible in light microscopic staining and scanning electron microscopy...
August 28, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28833678/nudix-type-rna-pyrophosphohydrolase-provides-homeostasis-of-virulence-factor-pyocyanin-and-functions-as-a-global-regulator-in-pseudomonas-aeruginosa
#5
Martyna Kujawa, Maciej Lirski, Mateusz Ziecina, Joanna Drabinska, Marta Modzelan, Elzbieta Kraszewska
The PA0336 protein from Pseudomonas aeruginosa belongs to the family of widely distributed Nudix pyrophosphohydrolases, which catalyze the hydrolysis of pyrophosphate bonds in a variety of nucleoside diphosphate derivatives. The amino acid sequence of the PA0336 protein is highly similar to that of the RppH Nudix RNA pyrophosphohydrolase from Escherichia coli, which removes pyrophosphate from 5'-end of triphosphorylated RNA transcripts. Trans-complementation experiments showed that the P. aeruginosa enzyme can functionally substitute for RppH in E...
August 18, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28799693/the-small-rna-real-a-novel-regulatory-element-embedded-in-the-pseudomonas-aeruginosa-quorum-sensing-networks
#6
Sara Carloni, Raffaella Macchi, Sara Sattin, Silvia Ferrara, Giovanni Bertoni
The small RNA ReaL of the opportunistic pathogen Pseudomonas aeruginosa has been characterized. Our results indicate that ReaL contributes to P. aeruginosa virulence. In the Galleria mellonella infection model, reaL gene deletion resulted in decreased virulence, while ReaL overexpression resulted in a hyper-virulent phenotype. We also demonstrate that ReaL is embedded in the P. aeruginosa quorum sensing (QS) with the role of linking las to pqs systems. We show that ReaL is negatively regulated by the las regulator LasR and impacts positively the synthesis of the pqs quinolone signal PQS by a positive post-transcriptional effect on the pqsC gene...
August 11, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28798903/anti-quorum-sensing-and-anti-biofilm-activity-of-delftia-tsuruhatensis-extract-by-attenuating-the-quorum-sensing-controlled-virulence-factor-production-in-pseudomonas-aeruginosa
#7
Vijay K Singh, Avinash Mishra, Bhavanath Jha
Multidrug-resistance bacteria commonly use cell-to-cell communication that leads to biofilm formation as one of the mechanisms for developing resistance. Quorum sensing inhibition (QSI) is an effective approach for the prevention of biofilm formation. A Gram-negative bacterium, Delftia tsuruhatensis SJ01, was isolated from the rhizosphere of a species of sedge (Cyperus laevigatus) grown along the coastal-saline area. The isolate SJ01 culture and bacterial crude extract showed QSI activity in the biosensor plate containing the reference strain Chromobacterium violaceum CV026...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28792229/eugenol-exhibits-anti-virulence-properties-by-competitively-binding-to-quorum-sensing-receptors
#8
Prasanth Rathinam, H S Vijay Kumar, Pragasam Viswanathan
The primary objective of this study was to ascertain the anti-biofilm and anti-virulence properties of sub-minimum inhibitory concentration (MIC) levels of eugenol against the standard strain PAO1 and two multi-drug resistant P. aeruginosa clinical isolates utilizing quorum sensing inhibition (QSI). Eugenol at 400 μM significantly reduced biofilm formation on urinary catheters and the virulence factors (VF) including extracellular polysaccharides, rhamnolipid, elastase, protease, pyocyanin, and pyoverdine (p < 0...
August 9, 2017: Biofouling
https://www.readbyqxmd.com/read/28757555/detection-of-pseudomonas-aeruginosa-metabolite-pyocyanin-in-water-and-saliva-by-employing-the-sers-technique
#9
Olga Žukovskaja, Izabella Jolan Jahn, Karina Weber, Dana Cialla-May, Jürgen Popp
Pyocyanin (PYO) is a metabolite specific for Pseudomonas aeruginosa. In the case of immunocompromised patients, it is currently considered a biomarker for life-threating Pseudomonas infections. In the frame of this study it is shown, that PYO can be detected in aqueous solution by employing surface-enhanced Raman spectroscopy (SERS) combined with a microfluidic platform. The achieved limit of detection is 0.5 μM. This is ~2 orders of magnitude below the concentration of PYO found in clinical samples. Furthermore, as proof of principle, the SERS detection of PYO in the saliva of three volunteers was also investigated...
July 25, 2017: Sensors
https://www.readbyqxmd.com/read/28755474/controlling-localization-of-escherichia-coli-populations-using-a-two-part-synthetic-motility-circuit-an-accelerator-and-brake
#10
Ryan McKay, Pricila Hauk, Hsuan-Chen Wu, Alex Eli Pottash, Wu Shang, Jessica Terrell, Gregory F Payne, William E Bentley
Probiotics, whether taken as capsules or consumed in foods, have been regarded as safe for human use by regulatory agencies. Being living cells, they serve as "tunable" factories for the synthesis of a vast array of beneficial molecules. The idea of reprogramming probiotics to act as controllable factories, producing potential therapeutic molecules under user-specified conditions, represents a new and powerful concept in drug synthesis and delivery. Probiotics that serve as drug delivery vehicles pose several challenges, one being targeting (as seen with nanoparticle approaches)...
July 29, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28747481/adaptation-to-public-goods-cheats-in-pseudomonas-aeruginosa
#11
Siobhán O'Brien, Adela M Luján, Steve Paterson, Michael A Cant, Angus Buckling
Cooperation in nature is ubiquitous, but is susceptible to social cheats who pay little or no cost of cooperation yet reap the benefits. The effect such cheats have on reducing population productivity suggests that there is selection for cooperators to mitigate the adverse effects of cheats. While mechanisms have been elucidated for scenarios involving a direct association between producer and cooperative product, it is less clear how cooperators may suppress cheating in an anonymous public goods scenario, where cheats cannot be directly identified...
July 26, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28738346/synergistic-activity-of-berberine-with-azithromycin-against-pseudomonas-aeruginosa-isolated-from-patients-with-cystic-fibrosis-of-lung-in-vitro-and-in-vivo
#12
YongTao Li, JianRong Huang, LanJuan Li, LinSheng Liu
BACKGROUND/AIMS: Pseudomonas aeruginosa (PA) is one of the major opportunistic pathogens which can cause chronic lung infection of cystic fibrosis (CF). The formation of PA biofilm promotes CF development and restricts the antimicrobial efficacies of current antibiotics. METHODS: The antimicrobial effects of azithromycin (AZM) and berberine (BER) alone and in combination were evaluated using microdilution method, checkerboard assay, time-kill test, qRT-PCR analysis and absorption method...
July 24, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28712963/anti-quorum-sensing-activity-of-pistacia-atlantica-against-pseudomonas-aeruginosa-pao1-and-identification-of-its-bioactive-compounds
#13
H Kordbacheh, F Eftekhar, S N Ebrahimi
Pseudomonas aeruginosa is a multidrug resistant opportunistic pathogen and an important cause of nosocomial infections. Quorum-sensing (QS) is a process in which bacterial cell-cell communication can regulates production of many virulence factors including pigment formation and the ability to form biofilm which is essential for establishment of chronic infections. We examined the inhibitory effect of Pistacia atlantica (Anacardiaceae) methanolic leaf extract and its bioactive components on biofilm formation and pigment production by P...
July 14, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28709085/amplification-of-electrochemical-signal-by-a-whole-cell-redox-reactivation-module-for-ultrasensitive-detection-of-pyocyanin
#14
Yuan Yang, Yang-Yang Yu, Yan-Zhai Wang, Chun-Lian Zhang, Jing-Xian Wang, Zhen Fang, Huoyang Lv, Jian-Jiang Zhong, Yang-Chun Yong
A bioelectrochemical sensing system based on a novel whole-cell redox reactivation/cycling module for ultrasensitive detection of pyocyanin (the biomarker of Pseudomonas aeruginosa infections) was developed. The electroactive bacteria mediated redox reactivation module was constructed using Shewanella oneidensis MR-1 cells as the bioelectro-catalyst and lactate as the electron donor. It could regenerate reductive pyocyanin from its oxidative state, which enabled pyocyanin molecule repeatedly registered by the electrode...
July 5, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28650325/evaluation-of-three-plant-extracts-against-biofilm-formation-and-expression-of-quorum-sensing-regulated-virulence-factors-in-pseudomonas-aeruginosa
#15
Vajihe Karbasizade, Parichehr Dehghan, Maryam Mohammadi Sichani, Kahin Shahanipoor, Reyhaneh Jafari, Rozita Yousefian
Following the increasing antibiotic resistance of pathogenic bacteria, the use of medicinal herbs as antibacterial agents has attracted growing attention. Pseudomonas aeruginosa is a human opportunistic pathogen that uses quorum sensing for regulating virulence gene expression (pyocyanin, protease, and elastase production and biofilm formation). This study examined the anti-quorum sensing activity of Quercus infectoria, Zataria multiflora and Trachyspermum copticum extracts on standard P. aeruginosa strain...
March 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28647320/an-integrated-aerobic-anaerobic-strategy-for-performance-enhancement-of-pseudomonas-aeruginosa-inoculated-microbial-fuel-cell
#16
Xiao-Yu Yong, Zhi-Ying Yan, Hai-Bo Shen, Jun Zhou, Xia-Yuan Wu, Li-Juan Zhang, Tao Zheng, Min Jiang, Ping Wei, Hong-Hua Jia, Yang-Chun Yong
Microbial fuel cell (MFC) is a promising device for energy generation and organic waste treatment simultaneously by electrochemically active bacteria (EAB). In this study, an integrated aerobic-anaerobic strategy was developed to improve the performance of P. aeruginosa-inoculated MFC. With an aerobic start-up and following an anaerobic discharge process, the current density of MFC reached a maximum of 99.80µA/cm(2), which was 91.6% higher than the MFC with conventional constant-anaerobic operation. Cyclic voltammetry and HPLC analysis showed that aerobic start-up significantly increased electron shuttle (pyocyanin) production (76% higher than the constant-anaerobic MFC)...
October 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28643380/microscopy-visualisation-confirms-multi-species-biofilms-are-ubiquitous-in-diabetic-foot-ulcers
#17
Khalid Johani, Matthew Malone, Slade Jensen, Iain Gosbell, Hugh Dickson, Honhua Hu, Karen Vickery
Increasing evidence within the literature has identified the presence of biofilms in chronic wounds and proposed that they contribute to delayed wound healing. This research aimed to investigate the presence of biofilm in diabetic foot ulcers (DFUs) using microscopy and molecular approaches and define if these are predominantly mono- or multi-species. Secondary objectives were to correlate wound observations against microscopy results in ascertaining if clinical cues are useful in detecting wound biofilm. DFU tissue specimens were obtained from 65 subjects...
June 23, 2017: International Wound Journal
https://www.readbyqxmd.com/read/28607159/mechanisms-of-pyocyanin-toxicity-and-genetic-determinants-of-resistance-in-staphylococcus-aureus
#18
Michael J Noto, William J Burns, William N Beavers, Eric P Skaar
Pseudomonas aeruginosa and Staphylococcus aureus are commonly isolated from polymicrobial infections, such as wound infections and chronic respiratory infections of persons with cystic fibrosis. Despite their co-isolation, P. aeruginosa produces substances toxic to S. aureus, including pyocyanin, a blue pigmented molecule that functions in P. aeruginosa virulence. Pyocyanin inhibits S. aureus respiration, forcing it to derive energy from fermentation and adopt a small colony variant (SCV) phenotype. The mechanisms by which S...
June 12, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28591849/pseudomonas-aeruginosa-quorum-sensing-response-in-the-absence-of-functional-lasr-and-lasi-proteins-the-case-of-strain-148-a-virulent-dolphin-isolate
#19
Estefanía Morales, Abigail González-Valdez, Luis Servín-González, Gloria Soberón-Chávez
Pseudomonas aeruginosa is an opportunistic pathogen that presents a complex regulatory network called 'quorum-sensing', which is responsible for the transcription of genes coding for several traits implicated in its pathogenicity. Strain 148 is a dolphin-isolate that has been shown to produce quorum-sensing regulated virulence-traits and to be virulent in a mouse model, despite the fact that it contains a 20 kbp deletion that eliminates from the chromosome the lasR gene and the lasI promoter. LasR is a key quorum-sensing transcriptional regulator that, when coupled with the autoinducer 3-oxo-dodecanoyl homoserine lactone (3O-C12-HSL) produced by LasI, activates transcription of genes coding for some virulence-associated traits such as elastase, lasI, rhlI and rhlR...
June 7, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28582765/new-applications-of-genetically-modified-pseudomonas-aeruginosa-for-toxicity-detection-in-water
#20
Dengbin Yu, Yang-Chun Yong, Changyu Liu, Youxing Fang, Lu Bai, Shaojun Dong
A novel mediator-free method based on genetically modified bacteria was developed for detecting water toxicity, where genetically modified Pseudomonas aeruginosa (GM P. aeruginosa) was selected as the biosensor strain and pyocyanin (PYO) produced by this strain was used as the indicator. The toxicity response of GM P. aeruginosa to 3, 5-dichlorophenol (3, 5-DCP) was measured electrochemically and spectroscopically, and the half maximal inhibitory concentration (IC50) of 3, 5-DCP was determined to be 15.1 mg/L...
October 2017: Chemosphere
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