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https://www.readbyqxmd.com/read/28094526/1h-nmr-based-global-metabolic-studies-of-pseudomonas-aeruginosa-upon-exposure-of-the-quorum-sensing-inhibitor-resveratrol
#1
Tongtong Chen, Jiyang Sheng, Yonghong Fu, Ming-Hui Li, Jun-Song Wang, Aiqun Jia
Quorum sensing (QS) is a process of bacterial communication that has been a novel target for drug discovery. Pyocyanin quantification assay confirmed that resveratrol was an effective quorum sensing inhibitor (QSI) against Pseudomonas aeruginosa PAO1. In this study, the global metabolite changes of P. aeruginosa PAO1 exposed to QSI resveratrol were investigated by 1H-NMR spectroscopy. A total of 40 metabolites containing amino acids, organic acid, organic amine and energy storage compounds were identified. The changed metabolic profile indicated that resveratrol influenced pathways including oxidative stress, protein synthesis and energy metabolism...
January 17, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28065820/bioavailability-enhanced-resveramax%C3%A2-modulates-quorum-sensing-and-inhibits-biofilm-formation-in-pseudomonas-aeruginosa-pao1
#2
H S Vasavi, H V Sudeep, H B Lingaraju, K Shyam Prasad
Quorum sensing (QS), a cell-to-cell communication mechanism present in many bacterial species plays a key role in regulating the virulence factor and biofilm formation in many pathogens, which causes severe public health risk. Therefore, interfering with QS mechanism offers an attractive strategy to combat bacterial infections. In the present study, anti-QS activity of a novel resveratrol formulation, Resveramax™, was detected using Chromobacterium violaceum biosensor bioassay and the effect of Resveramax on QS-regulated phenotypes in Pseudomonas aeruginosa PAO1 was assessed by standard protocols...
January 5, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28049617/synthesis-of-antimicrobial-glucosamides-as-bacterial-quorum-sensing-mechanism-inhibitors
#3
Nripendra N Biswas, Tsz Tin Yu, Önder Kimyon, Shashidhar Nizalapur, Christopher R Gardner, Mike Manefield, Renate Griffith, David StC Black, Naresh Kumar
Bacteria communicate with one another and regulate their pathogenicity through a phenomenon known as quorum sensing (QS). When the bacterial colony reaches a threshold density, the QS system induces the production of virulence factors and the formation of biofilms, a powerful defence system against the host's immune responses. The glucosamine monomer has been shown to disrupt the bacterial QS system by inhibiting autoinducer (AI) signalling molecules such as the acyl-homoserine lactones (AHLs). In this study, the synthesis of acetoxy-glucosamides 8, hydroxy-glucosamides 9 and 3-oxo-glucosamides 12 was performed via the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC·HCl) and N,N'-dicyclohexylcarbodiimide (DCC) coupling methods...
December 21, 2016: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28000767/a-diketopiperazine-factor-from-rheinheimera-aquimaris-qsi02-exhibits-anti-quorum-sensing-activity
#4
Shiwei Sun, Xiaoyun Dai, Jiao Sun, Xiangguo Bu, Caihong Weng, Hui Li, Hu Zhu
An ethyl acetate (EtOAc) extract isolated from the marine bacterium, Rheinheimera aquimaris QSI02, was found to exhibit anti-quorum sensing (anti-QS) activity. A subsequent bioassay-guided isolation protocol led to the detection of an active diketopiperazine factor, cyclo(Trp-Ser). Biosensor assay data showed that the minimum inhibitory concentration (MIC) of cyclo(Trp-Ser) ranged from 3.2 mg/ml to 6.4 mg/m for several microorganisms, including Escherichia coli, Chromobacterium violaceum CV026, Pseudomonas aeruginosa PA01, Staphylococcus aureus, and Candida albicans...
December 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27977197/disulfide-bond-containing-ajoene-analogues-as-novel-quorum-sensing-inhibitors-of-pseudomonas-aeruginosa
#5
July Fong, Mingjun Yuan, Tim Holm Jakobsen, Kim T Mortensen, May Margarette Salido Delos Santos, Song Lin Chua, Liang Yang, Choon Hong Tan, Thomas E Nielsen, Michael Givskov
Since its discovery 22 years ago, the bacterial cell-to-cell communication system, termed quorum sensing (QS), has shown potential as antipathogenic target. Previous studies reported that ajoene from garlic inhibits QS in opportunistic human pathogen Pseudomonas aeruginosa. In this study, screening of an in-house compound library revealed two sulfur-containing compounds which possess structural resemblance with ajoene and inhibit QS in bioreporter assay. Following a quantitative structure-activity relationship (SAR) study, 25 disulfide bond-containing analogues were synthesized and tested for QS inhibition activities...
December 28, 2016: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27940577/pyocyanin-degradation-by-a-tautomerizing-demethylase-inhibits-pseudomonas-aeruginosa-biofilms
#6
Kyle C Costa, Nathaniel R Glasser, Stuart J Conway, Dianne K Newman
The opportunistic pathogen Pseudomonas aeruginosa produces colorful redox-active metabolites called phenazines, which underpin biofilm development, virulence and clinical outcomes. Though phenazines exist in many forms, the best studied is pyocyanin. Here, we describe pyocyanin demethylase (PodA), a hitherto uncharacterized protein that oxidizes the pyocyanin methyl group to formaldehyde and reduces the pyrazine ring via an unusual tautomerizing demethylation reaction. Treatment with PodA disrupts P. aeruginosa biofilm formation similarly to DNase, suggesting interference with the pyocyanin-dependent release of extracellular DNA into the matrix...
December 8, 2016: Science
https://www.readbyqxmd.com/read/27933456/penicillin-v-acylases-from-gram-negative-bacteria-degrade-n-acylhomoserine-lactones-and-attenuate-virulence-in-pseudomonas-aeruginosa
#7
Avinash Vellore Sunder, Putri Dwi Utari, Sureshkumar Ramasamy, Ronald van Merkerk, Wim Quax, Archana Pundle
Virulence pathways in gram-negative pathogenic bacteria are regulated by quorum sensing mechanisms, through the production and sensing of N-acylhomoserine lactone (AHL) signal molecules. Enzymatic degradation of AHLs leading to attenuation of virulence (quorum quenching) could pave the way for the development of new antibacterials. Penicillin V acylases (PVAs) belong to the Ntn hydrolase superfamily, together with AHL acylases. PVAs are exploited widely in the pharmaceutical industry, but their role in the natural physiology of their native microbes is not clearly understood...
December 8, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27924027/crystal-structure-of-pseudomonas-aeruginosa-rsal-bound-to-promoter-dna-reaffirms-its-role-as-a-global-regulator-involved-in-quorum-sensing
#8
Huaping Kang, Jianhua Gan, Jingru Zhao, Weina Kong, Jing Zhang, Miao Zhu, Fan Li, Yaqin Song, Jin Qin, Haihua Liang
Pseudomonas aeruginosa possesses at least three well-defined quorum-sensing (QS) (las, rhl and pqs) systems that control a variety of important functions including virulence. RsaL is a QS repressor that reduces QS signal production and ensures homeostasis by functioning in opposition to LasR. However, its regulatory role in signal homeostasis remains elusive. Here, we conducted a ChIP-seq assay and revealed that RsaL bound to two new targets, the intergenic regions of PA2228/PA2229 and pqsH/cdpR, which are required for PQS synthesis...
October 18, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27923656/the-novel-antioxidant-ta293-reveals-the-role-of-cytoplasmic-hydroxyl-radicals-in-oxidative-stress-induced-senescence-and-inflammation
#9
Takahiro Sakai, Jun Imai, Tomohiro Ito, Hidetsugu Takagaki, Michio Ui, Shinichi Hatta
The hydroxyl radical (OH) possesses the strongest oxidation potential among reactive oxygen species (ROS). Hydroxyl radicals react nonpreferentially with proteins, lipids, and nucleic acids. Additionally, mitochondrial localization of OH causes dysfunction in the mitochondria. The cytoplasmic targets of OH-induced oxidation are unknown. No cytoplasm-specific OH scavenger is available; thus, elucidating the cytoplasmic targets of OH-induced oxidation has proven difficult. Accordingly, we developed a cytoplasm-specific OH-targeted scavenger, TA293, and a mitochondrion-specific scavenger, mitoTA293...
January 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27917856/biogenic-synthesis-of-zinc-oxide-nanostructures-from-nigella-sativa-seed-prospective-role-as-food-packaging-material-inhibiting-broad-spectrum-quorum-sensing-and-biofilm
#10
Nasser A Al-Shabib, Fohad Mabood Husain, Faheem Ahmed, Rais Ahmad Khan, Iqbal Ahmad, Edreese Alsharaeh, Mohd Shahnawaz Khan, Afzal Hussain, Md Tabish Rehman, Mohammad Yusuf, Iftekhar Hassan, Javed Masood Khan, Ghulam Md Ashraf, Ali Mohammed Alsalme, Mohamed F Al-Ajmi, Vadim V Tarasov, Gjumrakch Aliev
Bacterial spoilage of food products is regulated by density dependent communication system called quorum sensing (QS). QS control biofilm formation in numerous food pathogens and Biofilms formed on food surfaces act as carriers of bacterial contamination leading to spoilage of food and health hazards. Agents inhibiting or interfering with bacterial QS and biofilm are gaining importance as a novel class of next-generation food preservatives/packaging material. In the present study, Zinc nanostructures were synthesised using Nigella sativa seed extract (NS-ZnNPs)...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27902410/inhibition-of-quorum-sensing-controlled-virulence-factors-of-pseudomonas-aeruginosa-by-murraya-koenigii-essential-oil-a-study-in-a-caenorhabditis-elegans-infectious-model
#11
P Sankar Ganesh, Ravishankar Vittal Rai
The global emergence of antibiotic-resistant strains of Pseudomonas aeruginosa poses a major threat in both hospital environments and the community. P. aeruginosa is an opportunistic human pathogen, and it also infects a wide range of model organisms including the nematode Caenorhabditis elegans. Quorum sensing (QS) mediates cell-to-cell communication in bacteria and has an important role in regulating virulence genes, antibiotic resistance and biofilm formation, which are crucial for establishment of the infection...
December 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27900439/non-ribosomal-peptide-synthases-from-pseudomonas-aeruginosa-play-a-role-in-cyclodipeptide-biosynthesis-quorum-sensing-regulation-and-root-development-in-a-plant-host
#12
Omar González, Randy Ortíz-Castro, César Díaz-Pérez, Alma L Díaz-Pérez, Viridiana Magaña-Dueñas, José López-Bucio, Jesús Campos-García
Diverse molecules mediate cross-kingdom communication between bacteria and their eukaryotic partners and determine pathogenic or symbiotic relationships. N-acyl-L-homoserine lactone-dependent quorum-sensing signaling represses the biosynthesis of bacterial cyclodipeptides (CDPs) that act as auxin signal mimics in the host plant Arabidopsis thaliana. In this work, we performed bioinformatics, biochemical, and plant growth analyses to identify non-ribosomal peptide synthase (NRPS) proteins of Pseudomonas aeruginosa, which are involved in CDP synthesis...
November 30, 2016: Microbial Ecology
https://www.readbyqxmd.com/read/27874198/crataeva-nurvala-nanoparticles-inhibit-virulence-factors-and-biofilm-formation-in-clinical-isolates-of-pseudomonas-aeruginosa
#13
Syed Ghazanfar Ali, Mohammad Azam Ansari, Haris M Khan, Mohammad Jalal, Abbas Ali Mahdi, Swaranjit Singh Cameotra
Green synthesized nanoparticles have gained great attention due to their non-toxic and non-hazardous nature. In the present study, bark extract of the medicinal plant in Ayurveda Crataeva nurvala (Buch-Ham) (CN) was chosen for the biosynthesis of silver nanoparticles (AgNPs). These NPs were characterized by Ultra violet visible spectroscopy, Fourier Transform Infra Red, Atomic Force Microscopy, and Transmission Electron Microscopy (TEM). The average particle size of green synthesized CN-AgNPs was 15.2 ± 1...
November 22, 2016: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/27860087/the-effect-of-burdock-leaf-fraction-on-adhesion-biofilm-formation-quorum-sensing-and-virulence-factors-of-pseudomonas-aeruginosa
#14
Z Lou, H Wang, Y Tang, X Chen
AIMS: This study aimed to evaluate the effect of a fraction of burdock (Arctium lappa L.) leaf on the initial adhesion, biofilm formation, quorum sensing and virulence factors of Pseudomonas aeruginosa. METHODS AND RESULTS: Antibiofilm activity of the burdock leaf fraction was studied by the method of crystal violet staining. When the concentration of the burdock leaf fraction was 2·0 mg ml(-1) , the inhibition rates on biofilm formation of P. aeruginosa were 100%...
November 12, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27834332/attenuation-of-quorum-sensing-regulated-behaviour-by-tinospora-cordifolia-extract-identification-of-its-active-constituents
#15
Viraj C Gala, Nithya R John, Ashok M Bhagwat, Ajit G Datar, Prashant S Kharkar, Krutika B Desai
BACKGROUND & OBJECTIVES: The pathogenicity of the nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii is regulated by their quorum sensing (QS) systems. The objective of the present study was to examine the effect of the cold ethyl acetate extract of Tinospora cordifolia stem on virulence and biofilm development in the wild type and clinical strains of P. aeruginosa and A. baumannii. The study was further aimed to identify the probable active constituents in the plant extract...
July 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/27784787/epigallocatechin-gallate-remodels-overexpressed-functional-amyloids-in-pseudomonas-aeruginosa-and-increases-biofilm-susceptibility-to-antibiotic-treatment
#16
Marcel Stenvang, Morten S Dueholm, Brian S Vad, Thomas Seviour, Guanghong Zeng, Susana Geifman-Shochat, Mads T Søndergaard, Gunna Christiansen, Rikke Louise Meyer, Staffan Kjelleberg, Per Halkjær Nielsen, Daniel E Otzen
Epigallocatechin-3-gallate (EGCG) is the major polyphenol in green tea. It has antimicrobial properties and disrupts the ordered structure of amyloid fibrils involved in human disease. The antimicrobial effect of EGCG against the opportunistic pathogen Pseudomonas aeruginosa has been shown to involve disruption of quorum sensing (QS). Functional amyloid fibrils in P. aeruginosa (Fap) are able to bind and retain quorum-sensing molecules, suggesting that EGCG interferes with QS through structural remodeling of amyloid fibrils...
December 16, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27763824/blue-light-treatment-of-pseudomonas-aeruginosa-strong-bactericidal-activity-synergism-with-antibiotics-and-inactivation-of-virulence-factors
#17
Grzegorz Fila, Anna Kawiak, Mariusz Grinholc
Pseudomonas aeruginosa is among the most common pathogens responsible for both acute and chronic infections of high incidence and severity. Additionally, P. aeruginosa resistance to conventional antimicrobials has increased rapidly over the past decade. Therefore, it is crucial to explore new therapeutic options, particularly options that specifically target the pathogenic mechanisms of this microbe. The ability of a pathogenic bacterium to cause disease is dependent upon the production of agents termed 'virulence factors', and approaches to mitigate these agents have gained increasing attention as new antibacterial strategies...
October 20, 2016: Virulence
https://www.readbyqxmd.com/read/27761766/de-novo-synthesis-and-functional-analysis-of-polyphosphate-loaded-poly-ethylene-glycol-hydrogel-nanoparticles-targeting-pyocyanin-and-pyoverdin-production-in-pseudomonas-aeruginosa-as-a-model-intestinal-pathogen
#18
Yushu Yin, Georgia Papavasiliou, Olga Y Zaborina, John C Alverdy, Fouad Teymour
The human gastrointestinal tract is the primary site of colonization of multidrug resistant pathogens and the major source of life-threatening complications in critically ill and immunocompromised patients. Eradication measures using antibiotics carry further risk of antibiotic resistance. Furthermore, antibiotic treatment can adversely shift the intestinal microbiome toward domination by resistant pathogens. Therefore, approaches directed to prevent replacement of health promoting microbiota with resistant pathogens should be developed...
October 19, 2016: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/27725812/transcriptomic-and-metabolomic-analysis-revealed-multifaceted-effects-of-phage-protein-gp70-1-on-pseudomonas-aeruginosa
#19
Xia Zhao, Canhuang Chen, Xingyu Jiang, Wei Shen, Guangtao Huang, Shuai Le, Shuguang Lu, Lingyun Zou, Qingshan Ni, Ming Li, Yan Zhao, Jing Wang, Xiancai Rao, Fuquan Hu, Yinling Tan
The impact of phage infection on the host cell is severe. In order to take over the cellular machinery, some phage proteins were produced to shut off the host biosynthesis early in the phage infection. The discovery and identification of these phage-derived inhibitors have a significant prospect of application in antibacterial treatment. This work presented a phage protein, gp70.1, with non-specific inhibitory effects on Pseudomonas aeruginosa and Escherichia coli. Gp70.1 was encoded by early gene - orf 70...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27713984/heracleum-orphanidis-chemical-characterisation-and-comparative-evaluation-of-antioxidant-and-antimicrobial-activities-with-specific-interest-in-the-influence-on-pseudomonas-aeruginosa-pao1
#20
Ksenija S Mileski, Ana D Ćirić, Snežana S Trifunović, Mihailo S Ristić, Marina D Soković, Vlado S Matevski, Vele V Tešević, Milka B Jadranin, Petar D Marin, Ana M Džamić
GC-FID and GC-MS were used to determine the chemical composition of the essential oil of the aerial parts of Heracleum orphanidis Boiss. Also, the HPLC-DAD/ESI-ToF-MS profile of the methanol extracts of aerial parts and roots was determined. The main components of the essential oil were n-octanol, octyl hexanoate and n-octyl acetate, while coumarins were the most prevalent compounds in methanol extracts. An evaluation of antioxidant activity showed that the methanol and aqueous extracts of the aerial parts had the highest potential...
September 14, 2016: Food & Function
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