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https://www.readbyqxmd.com/read/29338525/pyocyanin-induces-systemic-oxidative-stress-inflammation-and-behavioral-changes-in-vivo
#1
Devinder Arora, Susan Hall, Shailendra Anoopkumar-Dukie, Rachel Morrison, Amelia McFarland, Anthony V Perkins, Andrew K Davey, Gary D Grant
Pyocyanin (PCN) is a virulence factor secreted by Pseudomonas aeruginosa (P. aeruginosa) that has been shown to have numerous toxic effects in both in vitro and in vivo studies. Such toxicities include pro-inflammatory and pro-oxidant mediated responses. It is hypothesized that PCN can cross biological membranes and reach the systemic circulation but no previous studies have investigated this. The aim of this study was, therefore, to quantify PCN in plasma and assess if systemic responses were occurring after localized intranasal administration in C57BL/6J mice...
January 16, 2018: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/29324344/anti-biofilm-effect-of-novel-thiazole-acid-analogs-against-pseudomonas-aeruginosa-through-iqs-pathways
#2
Shengrong Li, Siyu Chen, Jilin Fan, Zhen Cao, Weihao Ouyang, Ning Tong, Xin Hu, Jie Hu, Peishan Li, Zifeng Feng, Xi Huang, Yuying Li, Mingshan Xie, Ruikun He, Jingyi Jian, Biyuan Wu, Chen Xu, Weijian Wu, Jialiang Guo, Jing Lin, Pinghua Sun
IQS has been proven to be a new quorum sensing (QS) system against bacterial biofilm formation, which is activated in the common phosphate-limiting environment of infected tissues taking over the central las system. Up to now, numerous biofilm inhibitors which function by affecting traditional QS system have been reported. However, no compound has been reported to exert anti-biofilm activity through IQS system. Herein, various novel IQS derivatives were synthesized by the reaction of thiazole-4-carboxylic acid with different linear alcohols (R-OH) or amines (R-NH2)...
December 27, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29322006/attenuation-of-quorum-sensing-dependent-virulence-factors-and-biofilm-formation-by-medicinal-plants-against-antibiotic-resistant-pseudomonas-aeruginosa
#3
P Sankar Ganesh, V Ravishankar Rai
Pseudomonas aeruginosa use small signaling molecules such as acyl homoserine lactones (AHLs), which play an important role in release virulence factors and toxin for further establishment of host infection. Thus, involving with the QS system would provide alternative ways of preventing the pathogenicity. In the present study, totally six medicinal plants (Terminalia bellerica, Celastrus paniculatus, Kingiodendron pinnatum, Schleichera oleosa, Melastoma malabathricum, Garcinia gummi-gutta) were screened for anti-QS activity using biomonitor strain of Chromobacterium violaceum CV12472...
January 2018: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/29303546/establishment-of-lcms-based-platform-for-discovery-of-quorum-sensing-inhibitors-signal-detection-in-pseudomonas-aeruginosa-pao1
#4
Manoj Kushwaha, Shreyans K Jain, Nisha Sharma, Vidushi Abrol, Sundeep Jaglan, Ram A Vishwakarma
Targeting the main three networking systems viz. Las, RhI and PQS via natural quenchers is a new ray of hope for combating the persistent behavior of Pseudomonas aeruginosa. In the bacterial chemical vocabulary pyocyanin, N-AHLs and rhamnolipids are the main keywords, which are responsible for the social and nomadic behavior of P. aeruginosa. In the present work LCMS-based real-time qualitative and quantitative analysis of pyocyanin, green phenazine, N-AHLs, and rhamnolipids were performed. The quantitative analysis indicates that the production of pyocyanin and NHSLs increases with time while the production of rhamnolipids discontinued after 16 Hrs...
January 5, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29238468/effect-of-fetal-and-adult-bovine-serum-on-pyocyanin-production-in-pseudomonas-aeruginosa-isolated-from-clinical-and-soil-samples
#5
Aylin Moayedi, Jamileh Nowroozi, Abbas Akhavan Sepahy
Objectives: Pyocyanin is a blue-greenish redox-active pigment, produced by Pseudomonas aeruginosa, with a wide range of biological and biotechnological applications. Pyocyanin biosynthesis is regulated by the quorum-sensing (QS) system in which the expression of QS genes and QS-controlled virulence genes may be affected by serum as a complex medium. In the current study, effects of adult bovine serum (ABS) and fetal bovine serum (FBS) on the production of pyocyanin were examined in order to develop it...
December 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29201023/a-marine-actinomycete-rescues-caenorhabditis-elegans-from-pseudomonas-aeruginosa-infection-through-restitution-of-lysozyme-7
#6
Siti N Fatin, Tan Boon-Khai, Alexander Chong Shu-Chien, Melati Khairuddean, Amirul Al-Ashraf Abdullah
The resistance of Pseudomonas aeruginosa to conventional antimicrobial treatment is a major scourge in healthcare. Therefore, it is crucial that novel potent anti-infectives are discovered. The aim of the present study is to screen marine actinomycetes for chemical entities capable of overcoming P. aeruginosa infection through mechanisms involving anti-virulence or host immunity activities. A total of 18 actinomycetes isolates were sampled from marine sediment of Songsong Island, Kedah, Malaysia. Upon confirming that the methanolic crude extract of these isolates do not display direct bactericidal activities, they were tested for capacity to rescue Caenorhabditis elegans infected with P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29200950/isolation-and-determination-of-four-potential-antimicrobial-components-from-pseudomonas-aeruginosa-extracts
#7
Ling-Qing Xu, Jian-Wen Zeng, Chong-He Jiang, Huan Wang, Yu-Zhen Li, Wei-Hong Wen, Jie-Hua Li, Feng Wang, Wei-Jen Ting, Zi-Yong Sun, Chih-Yang Huang
Background:Pseudomonas aeruginosa can cause disease and also can be isolated from the skin of healthy people. Additionally, it exhibits certain antimicrobial effects against other microorganisms.Methods: We collected 60 strains of P. aeruginosa and screened their antimicrobial effects against Staphylococcus aureus (ATCC 25923) using the filter paper-disk method, the cross-streaking method and the co-culture method and then evaluated the antimicrobial activity of the chloroform-isolated S. aureus extracts against methicillin-resistant S...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29199454/effects-of-null-mutation-of-the-heat-shock-gene-htpg-on-the-production-of-virulence-factors-by-pseudomonas-aeruginosa
#8
Anna M Grudniak, Barbara Klecha, Krystyna I Wolska
AIM: Pseudomonas aeruginosa is one of the most clinically important opportunistic pathogen in humans. The aim of the project was to study effects of HtpG on the selected virulence factors responsible for pathogenesis and biofilm formation of P. aeruginosa. METHODOLOGY: By characterizing a htpG null mutant of P. aeruginosa, we have identified the role of HtpG in the production of selected factors. RESULTS: We showed that ΔhtpG mutant affects many physiological processes containing: decreased activity of the LasA protease, reduction of biofilm formation, decreased motility, and diminished amount of rhamnolipids and pyoverdine/pyocyanin...
December 4, 2017: Future Microbiology
https://www.readbyqxmd.com/read/29191704/microfouling-inhibition-of-human-nosocomial-pathogen-pseudomonas-aeruginosa-using-marine-cyanobacteria
#9
Felix LewisOscar, Chari Nithya, Sulaiman Ali Alharbi, Naiyf S Alharbi, Nooruddin Thajuddin
Pseudomonas aeruginosa is a Gram negative, opportunistic biofilm forming pathogenic bacterium which is developing as a serious problem worldwide. The pathogenicity of P. aeruginosa mainly depends upon biofilm and quorum sensing (QS) mechanism. Targeting biofilm and QS regulated factor will automatically reduce the pathogenicity of P. aeruginosa. Therefore it is compulsory to identify naturally derived biofilm and QS inhibitors against P. aeruginosa. In the present study Oscillatoria subuliformis, a marine cyanobacterium was used against the biofilm and QS of P...
November 27, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29151646/intracellular-synthesis-of-gold-nanoparticles-using-an-ectomycorrhizal-strain-em-1083-of-laccaria-fraterna-and-its-nanoanti-quorum-sensing-potential-against-pseudomonas-aeruginosa
#10
Sreeparna Samanta, Braj Raj Singh, Alok Adholeya
In this research work different shapes and sizes of gold nanoparticles (AuNPs) were synthesized through an intracellular biogenic approach, exploiting the chloroauric acid reducing and Au0 stabilizing potential of Laccaria fraterna EM-1083 mycelia. The intracellularly synthesized AuNPs exhibits anti-quorum sensing inhibitory potential against Pseudomonas aeruginosa. The synthesized AuNPs were characterized using UV-visible spectroscopy; transmission electron microscopy, X-ray diffraction, energy dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy...
December 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29130161/detection-of-2-alkyl-4-quinolones-using-biosensors
#11
Matthew P Fletcher, Stephen P Diggle, Miguel Cámara, Paul Williams
2-Alkyl-4-quinolones (AQs) such as 2-heptyl-3-hydroxy-4-quinolone (PQS) and 2-heptyl-4-hydroxyquinoline (HHQ) are quorum-sensing signal molecules. Here we describe two methods for AQ detection and quantification that employ thin-layer chromatography (TLC) and microtiter plate assays in combination with a lux-based Pseudomonas aeruginosa AQ biosensor strain. For TLC detection, organic solvent extracts of bacterial cells or spent culture supernatants are chromatographed on TLC plates, which are then dried and overlaid with the AQ biosensor...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29115136/kinetic-control-of-quorum-sensing-in-pseudomonas-aeruginosa-by-multidrug-efflux-pumps
#12
David Wolloscheck, Ganesh Krishnamoorthy, Jennifer Nguyen, Helen I Zgurskaya
Pseudomonas aeruginosa is an important human pathogen, the physiology and virulence of which are under the control of quorum sensing signals. These signals often have dual roles, functioning as toxins to some cells and as oxidative-stress protectors for their producer cells. Hence, their internal and external concentrations should be tightly controlled. In this study, we analyzed the interplay between the multidrug efflux transporters MexEF-OprN and MexG/HI-OpmD in quorum sensing of P. aeruginosa. We found that the two transporters have overlapping substrate specificities but different efficiencies...
November 10, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/29090193/reduced-intracellular-c-di-gmp-content-increases-expression-of-quorum-sensing-regulated-genes-in-pseudomonas-aeruginosa
#13
Song Lin Chua, Yang Liu, Yingying Li, Hui Jun Ting, Gurjeet S Kohli, Zhao Cai, Pipob Suwanchaikasem, Kelvin Kau Kit Goh, Sean Pin Ng, Tim Tolker-Nielsen, Liang Yang, Michael Givskov
Cyclic-di-GMP (c-di-GMP) is an intracellular secondary messenger which controls the biofilm life cycle in many bacterial species. High intracellular c-di-GMP content enhances biofilm formation via the reduction of motility and production of biofilm matrix, while low c-di-GMP content in biofilm cells leads to increased motility and biofilm dispersal. While the effect of high c-di-GMP levels on bacterial lifestyles is well studied, the physiology of cells at low c-di-GMP levels remains unclear. Here, we showed that Pseudomonas aeruginosa cells with high and low intracellular c-di-GMP contents possessed distinct transcriptome profiles...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29089927/autoinducer-2-facilitates-pseudomonas-aeruginosa-pao1-pathogenicity-in-vitro-and-in-vivo
#14
Hongdong Li, Xingyuan Li, Chao Song, Yunhui Zhang, Zhengli Wang, Zhenqiu Liu, Hong Wei, Jialin Yu
Bacterial communication systems, such as quorum sensing (QS), have provided new insights of alternative approaches in antimicrobial treatment. We recently reported that one QS signal, named as autoinducer-2 (AI-2), can affect the behaviors of Pseudomonas aeruginosa PAO1 in a dose-dependent manner. In this study, we aimed to investigate the effects of AI-2 on P. aeruginosa PAO1 biofilm formation and virulence factors production in vitro, and in vivo using a pulmonary infection mouse model. Exogenous AI-2 resulted in increased biofilms architecture, the number of viable cells, and the yield of pyocyanin and elastase virulence factors in wild type P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29042302/attenuation-of-pseudomonas-aeruginosa-quorum-sensing-virulence-and-biofilm-formation-by-extracts-of-andrographis-paniculata
#15
Malabika Banerjee, Soumitra Moulick, Kunal Kumar Bhattacharya, Debaprasad Parai, Subrata Chattopadhyay, Samir Kumar Mukherjee
Quorum-sensing (QS) is known to play an essential role in regulation of virulence factors and toxins during Pseudomonas aeruginosa infection which may frequently cause antibiotic resistance and hostile outcomes of inflammatory injury. Therefore, it is an urgent need to search for a novel agent with low risk of resistance development that can target QS and inflammatory damage prevention as well. Andrographis paniculata, a herbaceous plant under the family Acanthaceae, native to Asian countries and also cultivated in Scandinavia and some parts of Europe, has a strong traditional usage with its known antibacterial, anti-inflammatory, antipyretic, antiviral and antioxidant properties...
October 14, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29038052/serine-threonine-protein-kinase-ppka-contributes-to-the-adaptation-and-virulence-in-pseudomonas-aeruginosa
#16
Jianyi Pan, Zhenzhong Zha, Pengfei Zhang, Ran Chen, Chen Ye, Ting Ye
Pseudomonas aeruginosa is a leading cause of nosocomial infections worldwide and has become a serious public health threat, which is attributed to a large extent to its extraordinary environmental adaptability and diverse virulence factors that result in infection and progression of pathogenesis. The eukaryote-type serine/threonine (Ser/Thr) protein kinases, known for playing major regulatory roles in eukaryotes, have been demonstrated to play a central role in regulating various bacterial cellular processes via catalyzing protein phosphorylation...
October 13, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29026394/quorum-sensing-inhibitory-activity-of-sub-inhibitory-concentrations-of-%C3%AE-lactams
#17
Somaia A El-Mowafy, Khaled H Abd El Galil, El-Sayed E Habib, Mona I Shaaban
INTRODUCTION: The virulence factors of Pseudomonas aeruginosa are under the control of quorum sensing (QS) signals. Hence, interference with QS prevents its pathogenesis. OBJECTIVE: The aim of the present research is to assess the influence of some β-lactam antibiotics on cell communication and the release of different virulence factors. METHODS: The minimal inhibitory concentrations of ceftazidime, cefepime and imipenem were evaluated by microbroth dilution method...
March 2017: African Health Sciences
https://www.readbyqxmd.com/read/29018437/biocontrol-of-bacterial-leaf-blight-of-rice-and-profiling-of-secondary-metabolites-produced-by-rhizospheric-pseudomonas-aeruginosa-brp3
#18
Sumera Yasmin, Fauzia Y Hafeez, Muhammad S Mirza, Maria Rasul, Hafiz M I Arshad, Muhammad Zubair, Mazhar Iqbal
Xanthomonas oryzae pv. oryzae (Xoo) is widely prevalent and causes Bacterial Leaf Blight (BLB) in Basmati rice grown in different areas of Pakistan. There is a need to use environmentally safe approaches to overcome the loss of grain yield in rice due to this disease. The present study aimed to develop inocula, based on native antagonistic bacteria for biocontrol of BLB and to increase the yield of Super Basmati rice variety. Out of 512 bacteria isolated from the rice rhizosphere and screened for plant growth promoting determinants, the isolate BRp3 was found to be the best as it solubilized 97 μg/ mL phosphorus, produced 30 μg/mL phytohormone indole acetic acid and 15 mg/ L siderophores in vitro...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28993916/antimicrobial-metabolites-from-saraca-asoca-impairs-the-membrane-transport-system-and-quorum-sensing-system-in-pseudomonas-aeruginosa
#19
Preeti Jain, Amit Nale, Rajesh Dabur
This study was conducted to explore the antimicrobial mechanism of metabolites from Saraca asoca (SA1) using differential proteomics and metabolic profile of Pseudomonas aeruginosa after treatment with effective sub-MIC dose of 312 µg/mL. SA1 fraction was found to contain antibacterial metabolites catechol, protocatechuic acid, and epigallocatechin gallate. Proteome analysis revealed 33 differentially expressed proteins after SA1 treatment. Protein network analysis showed that SA1 treatment upregulated the DNA topological and metabolic processes...
October 9, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28983655/mechanistic-understanding-of-phenyllactic-acid-mediated-inhibition-of-quorum-sensing-and-biofilm-development-in-pseudomonas-aeruginosa
#20
Maitrayee Chatterjee, Sharon D'Morris, Vinod Paul, Sruthi Warrier, Anil Kumar Vasudevan, Muralidharan Vanuopadath, Sudarslal Sadasivan Nair, Bindhu Paul-Prasanth, C Gopi Mohan, Raja Biswas
Pseudomonas aeruginosa depends on its quorum sensing (QS) system for its virulence factors' production and biofilm formation. Biofilms of P. aeruginosa on the surface of indwelling catheters are often resistant to antibiotic therapy. Alternative approaches that employ QS inhibitors alone or in combination with antibiotics are being developed to tackle P. aeruginosa infections. Here, we have studied the mechanism of action of 3-Phenyllactic acid (PLA), a QS inhibitory compound produced by Lactobacillus species, against P...
October 5, 2017: Applied Microbiology and Biotechnology
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