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https://www.readbyqxmd.com/read/29199153/retrospective-assessment-of-an-assisted-reproductive-technology-method
#1
Siham Aboulmakarim, Amal Benbella, Houyam Hardizi, Rachid Bezad
The validation of methods in medical biology is a fundamental step in which the laboratory defines its analytical objectives, characterizes the performance of the technique according to the level of quality it fixes and discusses the results obtained. The objective of this work is to contribute to respect the requirements of ISO 15189 in terms of performance verification of a manual qualitative technique of medically assisted procreation (PMA): morphological identification of the oocyte, the zygote and the embryo, harmonization of professional practices and assessment of competences by associating the bibliographic approach and above all by justifying its choices...
December 4, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/29197809/a-model-for-measuring-the-environmental-sustainability-of-events
#2
Antonio Boggia, Gianluca Massei, Luisa Paolotti, Lucia Rocchi, Federico Schiavi
Like all human activities, events can generate significant pressures on environmental resources, unless they are well-managed and monitored. Therefore, it is becoming more and more important to develop models that can measure their environmental sustainability. Although increasing attention is being paid to this sector, there is currently no standard protocol or method to assess the eco-sustainability of events. This article presents an innovative assessment procedure to measure the environmental sustainability of events...
November 30, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/29188113/label-free-fiber-optic-optrode-for-the-detection-of-class-c-%C3%AE-lactamases-expressed-by-drug-resistant-bacteria
#3
Simona Zuppolini, Giuseppe Quero, Marco Consales, Laura Diodato, Patrizio Vaiano, Alberto Venturelli, Matteo Santucci, Francesca Spyrakis, Maria P Costi, Michele Giordano, Antonello Cutolo, Andrea Cusano, Anna Borriello
This paper reports the experimental assessment of an automated optical assay based on label free optical fiber optrodes for the fast detection of class C β-lactamases (AmpC BLs), actually considered as one of the most important sources of resistance to β-lactams antibiotics expressed by resistant bacteria. Reflection-type long period fiber gratings (RT-LPG) have been used as highly sensitive label free optrodes, while a higher affine boronic acid-based ligand was here selected to enhance the overall assay performances compared to those obtained in our first demonstration...
November 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/29182638/respondent-driven-sampling-for-identification-of-hiv-and-hcv-infected-people-who-inject-drugs-and-men-who-have-sex-with-men-in-india-a-cross-sectional-community-based-analysis
#4
Sunil S Solomon, Allison M McFall, Gregory M Lucas, Aylur K Srikrishnan, Muniratnam S Kumar, Santhanam Anand, Thomas C Quinn, David D Celentano, Shruti H Mehta
BACKGROUND: A major barrier to achieving ambitious targets for global control of HIV and hepatitis C virus (HCV) is low levels of awareness of infection among key populations such as men who have sex with men (MSM) and people who inject drugs (PWID). We explored the potential of a strategy routinely used for surveillance in these groups, respondent-driven sampling (RDS), to be used as an intervention to identify HIV- and HCV-infected PWID and MSM who are unaware of their status and those who are viremic across 26 Indian cities at various epidemic stages...
November 2017: PLoS Medicine
https://www.readbyqxmd.com/read/29166209/the-sexflex-scale-a-measure-of-sexual-script-flexibility-when-approaching-sexual-problems-in-a-relationship
#5
S Gauvin, C F Pukall
The objective of this paper was to develop a measure of sexual script flexibility when approaching a sexual problem in a relationship. A series of online studies were conducted with individuals in relationships. As one subscale on the two-factor model had poor convergent and discriminant validity, a final single factor scale with 6-items was retained. The single factor scale demonstrated good model fit, high internal reliability, adequate convergent and discriminant validity, and moderate test re-test reliability...
November 22, 2017: Journal of Sex & Marital Therapy
https://www.readbyqxmd.com/read/29165862/an-assessment-of-an-australasian-pathway-for-the-introduction-of-transanal-total-mesorectal-excision-tatme
#6
Sarah C Abbott, Andrew R L Stevenson, Stephen W Bell, David Clark, Arend Merrie, Julian Hayes, Shanthan Ganesh, Alexander G Heriot, Satish K Warrier
AIM: To evaluate the use of a pathway for the introduction of transanal total mesorectal excision (taTME) into Australia and New Zealand. METHOD: A pathway for surgeons with an appropriate level of specialist training and baseline skillset was initiated amongst colorectal surgeons; it includes an intensive course, a series of proctored cases and ongoing contribution to audit. Data were collected for patients who had taTME, for benign and malignant conditions, undertaken by the initial adopters of the technique...
November 22, 2017: Colorectal Disease: the Official Journal of the Association of Coloproctology of Great Britain and Ireland
https://www.readbyqxmd.com/read/29160426/impact-assessment-of-an-automated-drug-dispensing-system-in-a-tertiary-hospital
#7
Débora de-Carvalho, José Luiz Alvim-Borges, Cristiana Maria Toscano
OBJECTIVE: To evaluate the costs and patient safety of a pilot implementation of an automated dispensing cabinet in a critical care unit of a private tertiary hospital in São Paulo/Brazil. METHODS: This study considered pre- (January-August 2013) and post- (October 2013-October 2014) intervention periods. We considered the time and cost of personnel, number of adverse events, audit adjustments to patient bills, and urgent requests and returns of medications to the central pharmacy...
October 2017: Clinics
https://www.readbyqxmd.com/read/29157026/first-experiences-with-a-tool-to-measure-the-level-of-clinical-information-present-in-adverse-drug-reaction-reports
#8
Ingrid Oosterhuis, Leàn Rolfes, Corine Ekhart, Annemarie Muller-Hansma, Linda Härmark
BACKGROUND: To make a proper causality assessment of an adverse drug reaction (ADR) report, a certain level of clinical information is necessary. A tool was developed to measure the level of clinical information present in ADR reports. The aim of this study was to test the validity and reliability of the clinical documentation tool (ClinDoc) in an international setting. METHODS: The tool was developed by a panel of pharmacovigilance experts. It includes four domains: ADR, chronology of the ADR, suspected drug and patient characteristics...
November 20, 2017: Expert Opinion on Drug Safety
https://www.readbyqxmd.com/read/29126301/coagulation-testing-in-the-core-laboratory
#9
William E Winter, Sherri D Flax, Neil S Harris
Primary hemostasis begins with endothelial injury. VWF, produced by endothelial cells, binds to platelets and links them to subendothelial collagen. Platelet-derived ADP and thromboxane activate non-adhered platelets via their GPIIb/IIIa receptors, allowing these platelets to participate in platelet aggregation. Secondary hemostasis is initiated with the binding of factor VII to extravascular tissue factor (TF). Factors II, VII, IX and X are vitamin K-dependent factors. The role of vitamin K is to assist in the addition of gamma carboxylate groups to glutamic acids in the "GLA" domains of these factors...
November 8, 2017: Laboratory Medicine
https://www.readbyqxmd.com/read/29119652/the-novel-language-systematic-aphasia-screening-saps-screening-based-therapy-in-combination-with-computerised-home-training
#10
Franziska Krzok, Verena Rieger, Katharina Niemann, Ruth Nobis-Bosch, Irmgard Radermacher, Walter Huber, Klaus Willmes, Stefanie Abel
BACKGROUND: SAPS-'Sprachsystematisches Aphasiescreening'-is a novel language-systematic aphasia screening developed for the German language, which already had been positively evaluated. It offers a fast assessment of modality-specific psycholinguistic components at different levels of complexity and the derivation of impairment-based treatment foci from the individual performance profile. However, SAPS has not yet been evaluated in combination with the new SAPS-based treatment. AIMS: To replicate the practicality of SAPS and to investigate the effectiveness of a SAPS-based face-to-face therapy combined with computerised home training in a feasibility study...
November 8, 2017: International Journal of Language & Communication Disorders
https://www.readbyqxmd.com/read/29080409/evaluating-water-quality-and-ecotoxicology-assessment-techniques-using-data-from-a-lead-and-zinc-effected-upland-limestone-catchment
#11
Magaly Valencia-Avellan, Rebecca Slack, Anthony Stockdale, Robert John George Mortimer
Point and diffuse sources associated with historical metal ore mining are major causes of metal pollution. The understanding of metal behaviour and fate has been improved by the integration of water chemistry, metal availability and toxicity. Efforts have been devoted to the development of efficient methods of assessing and managing the risk posed by metals to aquatic life and meeting national water quality standards. This study focuses on the evaluation of current water quality and ecotoxicology techniques for the metal assessment of an upland limestone catchment located within a historical metal (lead ore) mining area in northern England...
October 15, 2017: Water Research
https://www.readbyqxmd.com/read/29080055/genetic-assessment-of-an-isolated-endemic-samango-monkey-cercopithecus-albogularis-labiatus-population-in-the-amathole-mountains-eastern-cape-province-south-africa
#12
M Thabang Madisha, Desire L Dalton, Raymond Jansen, Antoinette Kotze
The endemic Samango monkey subspecies (Cercopithecus albogularis labiatus) inhabits small discontinuous Afromontane forest patches in the Eastern Cape, KwaZulu-Natal midlands and southern Mpumalanga Provinces in South Africa. The subspecies is affected by restricted migration between forest patches which may impact on gene flow resulting in inbreeding and possible localized extinction. Current consensus, based on habitat quality, is that C. a. labiatus can be considered as endangered as the small forest patches they inhabit may not be large enough to sustain them...
October 27, 2017: Primates; Journal of Primatology
https://www.readbyqxmd.com/read/29068438/clinical-decision-making-observing-the-smartphone-user-an-observational-study-in-predicting-acute-surgical-patients-suitability-for-discharge
#13
Richard Hoffman, Simon Harley, Samuel Ellison, Peter G Devitt
INTRODUCTION: An accurate and rapid assessment of an acutely unwell patient's clinical status is paramount for the physician. There is an increasing trend to rely on investigations and results to inform a clinician of a patient's clinical status, with the subtleties of clinical observation often ignored. The aim of this study was to determine if a patient's use of a smartphone during the initial clinical assessment by a surgical consultant could be used as a surrogate marker for patient well-being, represented as their suitability for sameday discharge...
August 23, 2017: Journal of Hospital Medicine: An Official Publication of the Society of Hospital Medicine
https://www.readbyqxmd.com/read/29064169/unexpandable-lung-from-pleural-disease
#14
REVIEW
John T Huggins, Fabien Maldonado, Amit Chopra, Najib Rahman, Richard Light
Unexpandable lung is a common complication of malignant pleural effusions and inflammatory pleural diseases, such as pleural infection (e.g. empyema and complicated parapneumonic effusion) and noninfectious fibrinous pleuritis. Unexpandable lung due to pleural disease may be because of an active pleural process, and is referred to as malignant or inflammatory lung entrapment. An unexpandable lung may also be encountered in the setting of remote pleural inflammation resulting in a mature fibrous membrane overlying the visceral pleura preventing full expansion of the lung...
October 24, 2017: Respirology: Official Journal of the Asian Pacific Society of Respirology
https://www.readbyqxmd.com/read/29061347/reactogenicity-and-safety-assessment-of-an-attenuated-nanovaccine-against-scorpion-envenomation-preclinical-study
#15
Faez Amokrane Nait Mohamed, Abdelmounaim Nouri, Fatima Laraba-Djebari
An attenuated nanovaccine (Nps - V(∗)) has been developed to protect humans from fatal scorpion envenomation in at-risk regions. This study was conducted to evaluate the toxicity and the local reactogenicity of the Nps - V(∗) nanovaccine developed against Androctonus australis hector (Aah) venom. Assessment of the systemic inflammatory response and serum cytokine levels were evaluated in vaccinated mice with 100μg of irradiated Aah venom (V(∗)) encapsulated or not into polymeric calcium-alginate nanoparticles (Nps) and injected by subcutaneous (s...
October 20, 2017: Vaccine
https://www.readbyqxmd.com/read/29058471/assessment-of-the-validity-of-the-cudit-r-in-a-subpopulation-of-cannabis-users
#16
Mallory Loflin, Kimberly Babson, Kendall Browne, Marcel Bonn-Miller
BACKGROUND: The Cannabis Use Disorders Identification Test-Revised (CUDIT-R) is an 8-item measure used to screen for cannabis use disorders (CUD). Despite widespread use of the tool, assessments of the CUDIT-R's validity in subpopulations are limited. OBJECTIVES: Current study tested the structural validity and internal consistency of one of the most widely used screening measures for CUD (i.e., CUDIT-R) among a sample of military veterans who use cannabis for medicinal purposes...
October 23, 2017: American Journal of Drug and Alcohol Abuse
https://www.readbyqxmd.com/read/29054967/prediction-of-cytochrome-p450-mediated-drug-clearance-in-humans-based-on-the-measured-activities-of-selected-cyps
#17
Jie Gao, Jie Wang, Na Gao, Xin Tian, Jun Zhou, Yan Fang, Hai-Feng Zhang, Qiang Wen, Lin-Jing Jia, Dan Zou, Hai-Ling Qiao
Determining drug-metabolizing enzyme activities on an individual basis is an important component of personalized medicine, and cytochrome P450 enzymes (CYPs) play a principal role in hepatic drug metabolism. Herein, a simple method for predicting the major CYP-mediated drug clearance in vitro and in vivo is presented. Ten CYP-mediated drug metabolic activities in human liver microsomes (HLMs) from 105 normal liver samples were determined. The descriptive models for predicting the activities of these CYPs in HLMs were developed solely on the basis of the measured activities of a smaller number of more readily assayed CYPs...
October 20, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29047072/involving-members-of-the-public-in-health-economics-research-insights-from-selecting-health-states-for-valuation-to-estimate-quality-adjusted-life-year-qaly-weights
#18
Elizabeth Goodwin, Kate Boddy, Lynn Tatnell, Annie Hawton
Over recent years, public involvement in health research has expanded considerably. However, public involvement in designing and conducting health economics research is seldom reported. Here we describe the development, delivery and assessment of an approach for involving people in a clearly defined piece of health economics research: selecting health states for valuation in estimating quality-adjusted life-years (QALYs). This involvement formed part of a study to develop a condition-specific preference-based measure of health-related quality of life, the Multiple Sclerosis Impact Scale (MSIS-8D), and the work reported here relates to the identification of plausible, or realistic, health states for valuation...
October 19, 2017: Applied Health Economics and Health Policy
https://www.readbyqxmd.com/read/29042825/antithrombotics-in-trauma-management-strategies-in-the-older-patients
#19
REVIEW
Henna Wong, Nicola Lovett, Nicola Curry, Ku Shah, Simon J Stanworth
The ageing population has resulted in a change in the demographics of trauma, and older adult trauma now accounts for a growing number of trauma admissions. The management of older adult trauma can be particularly challenging, and exhibits differences to that of the younger age groups affected by trauma. Frailty syndromes are closely related with falls, which are the leading cause of major trauma in older adults. Comorbid disease and antithrombotic use are more common in the older population. Physiological changes that occur with ageing can alter the expected clinical presentation of older persons after injury and their susceptibility to injury...
2017: Journal of Blood Medicine
https://www.readbyqxmd.com/read/29041962/comparative-assessment-of-an-gambiae-and-an-stephensi-mosquitoes-to-determine-transmission-reducing-activity-of-antibodies-against-p-falciparum-sexual-stage-antigens
#20
Maarten Eldering, Anaïs Bompard, Kazutoyo Miura, Will Stone, Isabelle Morlais, Anna Cohuet, Geert-Jan van Gemert, Patrick M Brock, Sanna R Rijpma, Marga van de Vegte-Bolmer, Wouter Graumans, Rianne Siebelink-Stoter, Dari F Da, Carole A Long, Merribeth J Morin, Robert W Sauerwein, Thomas S Churcher, Teun Bousema
BACKGROUND: With the increasing interest in vaccines to interrupt malaria transmission, there is a demand for harmonization of current methods to assess Plasmodium transmission in laboratory settings. Potential vaccine candidates are currently tested in the standard membrane feeding assay (SMFA) that commonly relies on Anopheles stephensi mosquitoes. Other mosquito species including Anopheles gambiae are the dominant malaria vectors for Plasmodium falciparum in sub-Saharan Africa. METHODS: Using human serum and monoclonal pre-fertilization (anti-Pfs48/45) and post-fertilization (anti-Pfs25) antibodies known to effectively inhibit sporogony, we directly compared SMFA based estimates of transmission-reducing activity (TRA) for An...
October 17, 2017: Parasites & Vectors
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