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Staphylococcus epidermidis

Susana M M Lopes, Juliana S Novais, Dora C S Costa, Helena C Castro, Agnes Marie S Figueiredo, Vitor F Ferreira, Teresa M V D Pinho E Melo, Fernando de Carvalho da Silva
The generation and reactivity of 3-triazolyl-nitrosoalkenes are reported for the first time. The study showed that hetero-Diels-Alder reaction of these heterodienes is an interesting synthetic strategy to functionalized 1,2,3-triazoles, including 1,2,3-triazolyl-pyrroles, 1,2,3-triazolyl-dipyrromethanes and 1,2,3-triazolyl-indoles. The evaluation of the antibacterial profile against Gram-positive and Gram-negative strains revealed the new 5,5'-diethyldipyrromethane bearing a side chain incorporating a triazole and oxime moieties...
November 21, 2017: European Journal of Medicinal Chemistry
Virginia Carvalhais, Begoña Pérez-Cabezas, Cátia Oliveira, Rui Vitorino, Manuel Vilanova, Nuno Cerca
AIM: The goal of this study was to determine the effectiveness of antibiotics on Staphylococcus epidermidis biofilms with different proportions of dormant bacteria, using clinical and commensal isolates. MATERIALS & METHODS: The ability of S. epidermidis isolates to develop a dormant state was determined. The susceptibility of biofilms with prevented or induced dormancy to antibiotics was evaluated by enumeration of viable and cultivable cells, and confocal microscopy...
December 11, 2017: Future Microbiology
Morteza Vahedi, Nima Hosseini-Jazani, Saber Yousefi, Maryam Ghahremani
Background and Objectives: Staphylococcus epidermidis produces biofilm by extracellular polysaccharides, causing bacterial adherence to different surfaces. Anti-microbial effects of nickel nanoparticles on some bacterial strains such as S. aureus and Escherichia coli have been determined in limited studies. The aim of the present study is to examine the inhibitory effect of nickel nanoparticles on biofilm formation using clinical isolates of S. epidermidis and its hemolytic effect on human red blood cells...
June 2017: Iranian Journal of Microbiology
María Jesús Pérez-Granda, Beatriz Alonso, Ana Rodríguez-Huerta, Carmen Rodríguez, María Guembe
INTRODUCTION: Catheter-related bloodstream infection (C-RBSI) can sometimes be managed without catheter removal by combining systemic therapy with catheter lock therapy. Most antiseptic lock solutions are made up of ethanol combined with an anticoagulant. However, data regarding the anti-biofilm activity of ethanol combined with enoxaparin are scarce. We aimed to assess the efficacy of ethanol at different concentrations combined with enoxaparin 60IU as a lock solution for eradication of the biofilm of different microorganisms...
December 7, 2017: Enfermedades Infecciosas y Microbiología Clínica
Q Z Han, Y Chen, H Yang, X F Zhang, J Chen, D P Wu, S N Chen, H Y Qiu
Objective: To analyze the incidence and microbiological features of blood stream infections (BSI) of patients with hematopoietic stem cell transplantation (HSCT) and to provide laboratory data for empirical use of antibiotic for the HSCT patients with BSI. Methods: The incidence of bloodstream infection, the positive rate of blood culture, bacterial spectrum and drug resistance were analyzed in 1 265 HSCT recipients during 2013 and 2015 were retrospectively studied. Results: Of 1265 patients undergoing HSCT, 1 422 cases of suspected BSI occurred in 784 patients (61...
November 14, 2017: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
Brian Petrovsky, Eric Monnet
OBJECTIVE: To determine the efficacy of repeated decontamination and sterilization of a disposable port intended for 1-time use during single-incision laparoscopy. STUDY DESIGN: Experimental; prospective, controlled design. METHODS: Six single-access ports used 4 times and 6 single-access ports used 8 times to perform various clean, minimally invasive surgeries were evaluated. Ports were decontaminated in an enzymatic cleaner (dilution, 3:100) and cleaned with a scrub brush for 5 minutes...
December 8, 2017: Veterinary Surgery: VS
Ming Zhang, Yongqing Xu, Zhiming Jiang, Junying Qian, Zhonghui Zhang, Nannan Sun, Jian Xie, Tao Li
OBJECTIVE: To analyze the etiology and risk factors of central venous catheter (CVCs) infections, and to explore the prophylaxis and treatment for catheter-related infections. METHODS: A retrospective study was conducted. The patients with CVCs admitted to intensive care unit (ICU) of Qianfoshan Hospital Affiliated to Shandong University from January 2000 to December 2016 were enrolled. The gender, age, catheter data and microorganism culture results of all patients were collected...
December 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Didem Kart, Ayşe Semra Kustimur, Meral Sağıroğlu, Ayşe Kalkancı
BACKGROUND: Enterococcus faecalis, Escherichia coli, Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans biofilms are major causes of catheter-associated urinary tract infections. Antimicrobial-coated or impregnated urinary catheters are seen as a possible way to prevent these infections. AIMS: To determine the biofilm-forming ability of 89 E. faecalis isolates from urinary tract infections and to compare several urinary catheters for antimicrobial durability and the inhibitory effects on biofilm formation of different laboratory strains and clinical isolates of E...
December 1, 2017: Balkan Medical Journal
Vivek Yadav, Yuly Andrea Jaimes-Lizcano, Narendra K Dewangan, Nayoung Park, Tzu-Han Li, Megan L Robertson, Jacinta C Conrad
We investigated the effect of two brush parameters, thickness and dispersity in the molecular weight distribution, on the adhesion of bacteria to pH-responsive poly(acrylic acid) (PAA) brushes synthesized using surface-initiated atom transfer radical polymerization. The attachment and detachment of Staphylococcus epidermidis to PAA brushes at pH 4 and pH 9, respectively, were examined with confocal microscopy. An optimal range of brush thickness, 13 - 18 nm, was identified for minimizing bacterial adhesion on PAA brushes at pH 4, and bacterial attachment did not depend on the brush dispersity...
December 7, 2017: ACS Applied Materials & Interfaces
Behrooz Alizadeh Behbahani, Abbas Ali Imani Fooladi
The aim of this study was to investigate the antibacterial activities and phytochemical analysis of extracts against the growth of some pathogenic strain causing poisoning and infection (Staphylococcus aureus, Streptococcus pyogenes, Staphylococcus epidermidis, Enterobacter aerogenes, Escherichia coli and Shigella flexneri). Makhlaseh components were identified via gas chromatography/mass spectrometry (GC/MS). Total phenolic content (TPC), alkaloids, tannins and saponins were determined. Antioxidant activity was determined calorimetrically for 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity...
December 1, 2017: Microbial Pathogenesis
Ronan M N Noble, Saad Y Salim, Bradley Walker, Rachel G Khadaroo, Angelo B Chiarella, Ferrante S Gragasin, Stephane L Bourque
We tested whether propofol or Intralipid inoculated with Staphylococcus epidermidis would promote bacterial growth within an intravenous (IV) injection hub, a site prone to bacterial contamination. In tubes incubated under optimal conditions, S epidermidis exhibited growth in Intralipid, but not in propofol. In contrast, within the IV hub incubated with either propofol or intralipid at room temperature, S epidermidis bacterial numbers declined with time, and virtually no contamination remained after 12 hours...
November 30, 2017: Anesthesia and Analgesia
Aimée Maria Guiotti, Emily Vivianne Freitas da Silva, Isabela Araguê Catanoze, Karina Helga Leal Turcio, Estefânia Marrega Malavazi, Marcelo Coelho Goiato, Daniela Micheline Dos Santos, Margarete Teresa Gottardo de Almeida
The purpose of this study was to identify and analyze the microorganisms present in the conjunctival secretion in anophthalmic cavities of wearers of ocular prostheses, as well as on the prostheses used by them, correlating them with the microbiota of the contralateral eye. Nine patients with maxillofacial abnormalities, wearers of an acrylic resin ocular prosthesis participated in the study. Collections of conjunctival secretions and biofilm were performed on the prosthesis, anophthalmic cavity, and contralateral eye for the mycological and bacterial analyses...
November 29, 2017: Letters in Applied Microbiology
Bujung Hong, Andreas Winkel, Philipp Ertl, Sascha Nico Stumpp, Kerstin Schwabe, Meike Stiesch, Joachim K Krauss
BACKGROUND: Wound healing impairment is a serious problem in surgical disciplines which may be associated with chronic morbidity, increased cost and patient discomfort. Here we aimed to investigate the relevance of bacterial colonisation on suture material using polymerase chain reaction (PCR) to detect and taxonomically classify bacterial DNA in patients with and without wound healing problems after routine neurosurgical procedures. METHODS: Repeat surgery was performed in 25 patients with wound healing impairment and in 38 patients with well-healed wounds...
November 30, 2017: Acta Neurochirurgica
Bushra Uzair, Neelam Firdous, Barkat Ali Khan, Samiullah Khan, Sammer Fatima, Rehana Kausar, Asma Bano
The emergence of multi drug resistant microbial pathogens has become a global health challenge and set a dire requirement of searching new effective antimicrobials. Soil is an ultimate reservoir of biologically active micro flora, which harbors trillions of microbial strains producing compounds of commercial interest. Hence aim of the present study was an attempt to isolate and identify the antibiotic producing microbial strains from the red soil of Himalayan an unexplored region of Pakistan. In this study from 10 different soil samples only one bacterial strain was isolated capable of antimicrobial activity...
November 2017: Pakistan Journal of Pharmaceutical Sciences
Brandon Brooks, Matthew R Olm, Brian A Firek, Robyn Baker, Brian C Thomas, Michael J Morowitz, Jillian F Banfield
Preterm infants exhibit different microbiome colonization patterns relative to full-term infants, and it is speculated that the hospital room environment may contribute to infant microbiome development. Here, we present a genome-resolved metagenomic study of microbial genotypes from the gastrointestinal tracts of infants and from the neonatal intensive care unit (NICU) room environment. Some strains detected in hospitalized infants also occur in sinks and on surfaces, and belong to species such as Staphylococcus epidermidis, Enterococcus faecalis, Pseudomonas aeruginosa, and Klebsiella pneumoniae, which are frequently implicated in nosocomial infection and preterm infant gut colonization...
November 27, 2017: Nature Communications
Sabina Horvat, Aleksander Mahnic, Martin Breskvar, Saso Dzeroski, Maja Rupnik
Clostridium difficile infection (CDI) is typically associated with disturbed gut microbiota and changes related to decreased colonization resistance against C. difficile are well described. However, nothing is known about possible effects of C. difficile on gut microbiota restoration during or after CDI. In this study, we have mimicked such a situation by using C. difficile conditioned medium of six different C. difficile strains belonging to PCR ribotypes 027 and 014/020 for cultivation of fecal microbiota...
November 27, 2017: Scientific Reports
Alisa Wilantho, Pamornya Deekaew, Chutika Srisuttiyakorn, Sissades Tongsima, Naraporn Somboonna
Background: Skin microbiome varies from person to person due to a combination of various factors, including age, biogeography, sex, cosmetics and genetics. Many skin disorders appear to be related to the resident microflora, yet databases of facial skin microbiome of many biogeographies, including Thai, are limited. Methods: Metagenomics derived B-RISA and 16S rRNA gene sequencing was utilized to identify the culture-independent bacterial diversity on Thai male faces (cheek and forehead areas)...
2017: PeerJ
Dayara Normando Marques, Alexandra Sampaio de Almeida, Andressa Rocha de Oliveira Sousa, Rafael Pereira, Alexandre Lopes Andrade, Renata Pinheiro Chaves, Rômulo Farias Carneiro, Mayron Alves de Vasconcelos, Luiz Gonzaga Ndo Nascimento-Neto, Ulisses Pinheiro, Paula Alexandra Videira, Edson Holanda Teixeira, Celso Shiniti Nagano, Alexandre Holanda Sampaio
A new lectin from the marine sponge Chondrilla caribensis (CCL) was isolated by affinity chromatography in Sepharose 6B media. CCL is a homotetrameric protein formed by subunits of 15,445 ±2Da. The lectin showed affinity for disaccharides containing galactose and mucin. Mass spectrometric analysis revealed about 50% of amino acid sequence of CCL, which showed similarity with a lectin isolated from Aplysina lactuca. Secondary structure consisted of 10% α-helix, 74% β-sheet/β-turn and 16% coil, and this profile was unaltered in a broad range of pH and temperatures...
November 22, 2017: International Journal of Biological Macromolecules
Weslley F Oliveira, Priscila M S Silva, Rayane C S Silva, Germana M M Silva, Giovanna Machado, Luana C B B Coelho, Maria T S Correia
BACKGROUND: Infections are one of the main reasons for removal of implants from patients, and usually need difficult and expensive treatments. Staphylococcus aureus and Staphylococcus epidermidis are the most frequent pathogens. AIM: To review the epidemiology and pathogenesis of implant-related infections. METHODS: Relevant studies were identified by electronic searching of the following databases: PubMed, ScienceDirect, Academic Google and CAPES Journal Portal...
November 21, 2017: Journal of Hospital Infection
A-K Nyman, C Fasth, K Persson Waller
Subclinical mastitis causes an increase in milk somatic cell count (SCC) and can lead to reduced milk production and early culling. In many countries, non-aureus staphylococci (NAS) is the most common bacterial finding in subclinical mastitis of dairy cows. New methodology makes it possible to identify NAS species, but knowledge about the epidemiology is limited. The objective of this project was to improve advisory services for mastitis control by investigating associations between NAS and SCC, milk production, and persistence of intramammary infections (IMI)...
November 22, 2017: Journal of Dairy Science
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