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Staphylococcus epidermidis

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https://www.readbyqxmd.com/read/28214187/microbiological-analysis-antimicrobial-activity-and-heavy-metals-content-of-jordanian-ma-in-hot-springs-water
#1
Muhamad Ali K Shakhatreh, Jacob H Jacob, Emad I Hussein, Majed M Masadeh, Safwan M Obeidat, Abdul-Salam F Juhmani, Mutaz A Abd Al-Razaq
Ma'in hot springs are known as sites of balneotherapy. However, little is known about their microbiology and chemistry. In this study, we aim at evaluating the antimicrobial activity of Ma'in hot-springs water (MHSW), studying its microbiology, and determining its physicochemical properties including the heavy metals content. Therefore, water samples were collected from Ma'in hot springs and tested for antimicrobial activity using agar diffusion method. Water was then cultivated on nutrient agar to isolate and identify the dominant bacteria by chemical and molecular methods...
February 14, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28211364/antibacterial-effect-of-novel-biodegradable-and-bioresorbable-plda-mg-composites
#2
M C Fernández-Calderón, S C Cifuentes, M A Pacha-Olivenza, A M Gallardo-Moreno, L Saldaña, J L González-Carrasco, M T Blanco, N Vilaboa, M L González-Martín, C Pérez-Giraldo
Polylactic acid/Mg composites have been recently proposed for biodegradable osteosynthesis devices because, with regards to the neat polymer, they combine an enhanced biocompatibility and bioactivity with better mechanical properties, particularly creep strength. A question still arises about their bacterial behavior. For this purpose, composites of poly-L-D-lactic acid (PLDA) loaded with 1 and 10 wt.% of Mg microparticles were evaluated using Staphylococcus epidermidis, with special emphasis on the study of bacterial adhesion and biofilm formation...
February 17, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28207943/the-role-of-the-skin-microbiome-in-atopic-dermatitis-a-systematic-review
#3
REVIEW
R Dybboe, J Bandier, L Skov, L Engstrand, J D Johansen
BACKGROUND: Dysbiosis is a hallmark of atopic dermatitis. The composition of skin microbiome communities and the causality of dysbiosis in eczema have not been well established. OBJECTIVE: To describe the skin microbiome profile in atopic dermatitis and address if there is a causal relationship between dysbiosis and atopic dermatitis. METHODS: The protocol is registered in PROSPERO (CRD42016035813). We searched PubMed, Embase, Scopus and ClinicalTrials...
February 16, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/28203330/risk-factors-for-health-care-associated-bloodstream-infections-in-a-neonatal-intensive-care-unit
#4
Sabahattin Ertugrul, Fesih Aktar, Ilyas Yolbas, Ahmet Yilmaz, Bilal Elbey, Ahmet Yildirim, Kamil Yilmaz, Recep Tekin
BACKGROUND: Healthcare-associated bloodstream infections (HCA-BSI) are a major cause of morbidity and mortality in neonatal intensive care units (NICUs). OBJECTIVES: We aimed to determine the causative organisms and risk factors of HCA-BSIs in NICUs. METHODS: This study was performed between January 2011 and December 2014 in the neonatal intensive care unit of Dicle university, Turkey. The study consisted of 126 patients (infected group) with positive blood culture and 126 randomly selected patients (uninfected control group) with negative blood culture after four days of hospitalization...
October 2016: Iranian Journal of Pediatrics
https://www.readbyqxmd.com/read/28203232/cationic-net-charge-and-counter-ion-type-as-antimicrobial-activity-determinant-factors-of-short-lipopeptides
#5
Katarzyna E Greber, Malgorzata Dawgul, Wojciech Kamysz, Wieslaw Sawicki
To get a better insight into the antimicrobial potency of short cationic lipopeptides, 35 new entities were synthesized using solid phase peptide strategy. All newly obtained lipopeptides were designed to be positively charged from +1 to +4. This was achieved by introducing basic amino acid - lysine - into the lipopeptide structure and had a hydrophobic fatty acid chain attached. Lipopeptides were subjected to microbiological tests using reference strains of Gram-negative bacteria: Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Gram-positive bacteria: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Enterococcus faecalis, and fungi: Candida albicans, Candida tropicalis, Aspergillus brasiliensis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28197394/antibiogram-of-bacteria-isolated-from-automated-teller-machines-in-hamadan-west-iran
#6
Hassan Mahmoudi, Mohammad Reza Arabestani, Mohammad Yousef Alikhani, Iraj Sedighi, Hamed Farhadi Kohan, Mohammad Molavi
Aim: Bacteria are ubiquitous in the environment. In keeping with the continued expansion of urbanization and the growing population, an increasing number of people use automated banking, i.e. automated teller machines (ATMs). The aim of this study was to investigate the bacterial contamination and its antibiotic sensitivity on computer keyboards located at ATMs in Hamadan province, Iran. Method: Out of 360 ATMs at four locations in Hamadan, 96 were randomly selected for this study. The antibiotic susceptibility pattern of all isolates was determined by the agar disk diffusion method using gentamicin (10 µg), vancomycin (30 µg), trimethoprim/sulfamethoxazole (25 µg), amikacin (30 µg), tobramycin (10 µg), cephalotin (30 µg), norfloxacin (5 µg), and ceftizoxim (30 µg) disks...
2017: GMS Hygiene and Infection Control
https://www.readbyqxmd.com/read/28194715/does-extracellular-dna-production-vary-in-staphylococcal-biofilms-isolated-from-infected-implants-versus-controls
#7
Beata Zatorska, Marion Groger, Doris Moser, Magda Diab-Elschahawi, Luigi Segagni Lusignani, Elisabeth Presterl
BACKGROUND: Prosthetic implant infections caused by Staphylococcus aureus and epidermidis are major challenges for early diagnosis and treatment owing to biofilm formation on the implant surface. Extracellular DNA (eDNA) is actively excreted from bacterial cells in biofilms, contributing to biofilm stability, and may offer promise in the detection or treatment of such infections. QUESTIONS/PURPOSES: (1) Does DNA structure change during biofilm formation? (2) Are there time-dependent differences in eDNA production during biofilm formation? (3) Is there differential eDNA production between clinical and control Staphylococcal isolates? (4) Is eDNA production correlated to biofilm thickness? METHODS: We investigated eDNA presence during biofilm formation in 60 clinical and 30 control isolates of S aureus and S epidermidis...
February 13, 2017: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/28194136/substance-p-and-calcitonin-gene-related-peptide-key-regulators-of-cutaneous-microbiota-homeostasis
#8
REVIEW
Awa N'Diaye, Andrei Gannesen, Valérie Borrel, Olivier Maillot, Jeremy Enault, Pierre-Jean Racine, Vladimir Plakunov, Sylvie Chevalier, Olivier Lesouhaitier, Marc G J Feuilloley
Neurohormones diffuse in sweat and epidermis leading skin bacterial microflora to be largely exposed to these host factors. Bacteria can sense a multitude of neurohormones, but their role in skin homeostasis was only investigated recently. The first study focused on substance P (SP), a neuropeptide produced in abundance by skin nerve terminals. SP is without effect on the growth of Gram-positive (Bacillus cereus, Staphylococcus aureus, and Staphylococcus epidermidis) and Gram-negative (Pseudomonas fluorescens) bacteria...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28191348/pediatric-posttraumatic-endophthalmitis-in-china-for-twenty-years
#9
REVIEW
Yan Sheng, Wen Sun, Yangshun Gu, Andrzej Grzybowski
Pediatric posttraumatic endophthalmitis (PTE) is a rare but serious disease that frequently has a poor visual prognosis. To date, only a few English studies have focused on this disease. We perform a systematic review of the Chinese literature on pediatric PTE and describe the epidemiology, management, causative organisms, and visual acuity outcomes of reported cases in twenty years. We found that Staphylococcus epidermidis was the most common isolated organism and the use of a disposable syringe needle was the most common cause of ocular injuries in pediatric PTE in China...
2017: Journal of Ophthalmology
https://www.readbyqxmd.com/read/28187327/staphylococcal-ecosystem-of-kitoza-a-traditional-malagasy-meat-product
#10
Angela Ratsimba, Sabine Leroy, Jean Paul Chacornac, Danielle Rakoto, Elodie Arnaud, Victor Jeannoda, Régine Talon
Kitoza is a traditional meat product from Madagascar manufactured with strips of pork or beef. The process includes a first step of salting and mixing with spices followed by sun-drying or smoking step. As salting and drying select coagulase-negative staphylococci (CNS), our aim was to identify the CNS species in kitoza with the objective in the future of developing indigenous starters. Microbial analyses revealed that the only pathogenic bacterium enumerated was Staphylococcus aureus, which was found in 54% of the samples...
February 2, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28179897/comparative-transcriptomics-reveals-discrete-survival-responses-of-s-aureus-and-s-epidermidis-to-sapienic-acid
#11
Josephine C Moran, Jamal A Alorabi, Malcolm J Horsburgh
Staphylococcal colonization of human skin is ubiquitous, with particular species more frequent at different body sites. Whereas Staphylococcus epidermidis can be isolated from the skin of every individual tested, Staphylococcus aureus is isolated from <5% of healthy individuals. The factors that drive staphylococcal speciation and niche selection on skin are incompletely defined. Here we show that S. aureus is inhibited to a greater extent than S. epidermidis by the sebaceous lipid sapienic acid, supporting a role for this skin antimicrobial in selection of skin staphylococci...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28175925/skin-microbiome-in-small-and-large-plaque-parapsoriasis
#12
Alexander Salava, Pedro Pereira, Velma Aho, Liisa Väkevä, Lars Paulin, Petri Auvinen, Annamari Ranki, Antti Lauerma
Staphylococcal enterotoxins have been shown to promote lymphoma-associated immune dysregulation. This study examined changes in the skin microbiome of parapsoriasis compared with intact skin. Swab microbiome specimens were taken of the parapsoriasis lesions of 13 patients. Control samples were taken from contralateral healthy sides of the body. Microbiotas were characterized by sequencing the V1-V3 region of the 16S ribosomal RNA bacterial genes on the Illumina MiSeq platform. The most common genera in the microbiome data were Propionibacterium (27...
February 8, 2017: Acta Dermato-venereologica
https://www.readbyqxmd.com/read/28168343/is-infection-the-possible-initiator-of-disc-disease-an-insight-from-proteomic-analysis
#13
S Rajasekaran, Chitraa Tangavel, Siddharth N Aiyer, Sharon Miracle Nayagam, M Raveendran, Naveen Luke Demonte, Pramela Subbaiah, Rishi Kanna, Ajoy Prasad Shetty, K Dharmalingam
STUDY DESIGN: Proteomic and 16S rDNA analysis of disc tissues obtained in vivo. OBJECTIVE: To address the controversy of infection as an aetiology for disc disorders through protein profiling. There is raging controversy over the presence of bacteria in human lumbar discs in vivo, and if they represent contamination or infection. Proteomics can provide valuable insight by identifying proteins signifying bacterial presence and, also host defence response proteins (HDRPs), which will confirm infection...
February 6, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28166134/nipple-shields-in-transaxillary-breast-augmentation
#14
Jesús Benito-Ruiz
BACKGROUND: Infection after breast augmentation occurs in 1.1% to 2.5% of patients. Bacterial contamination of the implants could explain some complications of breast implant surgery, including infection, capsular contracture and even anaplastic large cell lymphoma. Because of the evidence of bacterial spread from the nipple, nipple shields have been proposed as a routine maneuver to avoid contamination of the implants. OBJECTIVE: To determine if nipple shields are useful in transaxillary breast augmentation...
February 4, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28163960/screening-for-antibacterial-and-antibiofilm-activities-in-astragalus-angulosus
#15
Hussein Kanaan, Majid El-Mestrah, Alaa Sweidan, Falah As-Sadi, Ali Al Bazzal, Ali Chokr
AIM: In a search for finding novel therapeutic agents, extracts from an endemic Lebanese plant, Astragalus angulosus, were evaluated for their potential in-vitro antibacterial and antibiofilm activities against three Gram-positive bacterial strains; Staphylococcus epidermidis (CIP444), Staphylococcus aureus (ATCC25923), and Enterococcus faecalis (ATCC29212); in addition to two Gram-negative strains, Escherichia coli (ATCC35218) and Pseudomonas aeruginosa (ATCC27853). MATERIALS AND METHODS: The plant was collected in April of 2013 and divided into several different portions, then its extracts were obtained by maceration using two different solvents...
January 2017: Journal of Intercultural Ethnopharmacology
https://www.readbyqxmd.com/read/28158617/exploring-the-pharmacodynamic-interactions-between-tedizolid-and-other-orally-bioavailable-antimicrobials-against-staphylococcus-aureus-and-staphylococcus-epidermidis
#16
Brian J Werth
No abstract text is available yet for this article.
January 31, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28155128/lactobacillus-plantarum-as-a-probiotic-potential-from-kouzeh-cheese-traditional-iranian-cheese-and-its-antimicrobial-activity
#17
Vahid Jabbari, Mahmoud Sowti Khiabani, Reza Rezaei Mokarram, Azad Mohammad Hassanzadeh, Elham Ahmadi, Sasan Gharenaghadeh, Nayyer Karimi, Hossein Samadi Kafil
The aim of this study is to isolate and identify Lactobacillus plantarum isolates from traditional cheese, Kouzeh, and evaluate their antimicrobial activity against some food pathogens. In total, 56 lactic acid bacteria were isolated by morphological and biochemical methods, 12 of which were identified as Lactobacillus plantarum by biochemical method and 11 were confirmed by molecular method. For analyzing the antimicrobial activity of these isolates properly, diffusion method was performed. The isolates were identified by 318 bp band dedicated for L...
February 2, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28151994/toxin-mediates-sepsis-caused-by-methicillin-resistant-staphylococcus-epidermidis
#18
Li Qin, Fei Da, Emilie L Fisher, Daniel C S Tan, Thuan H Nguyen, Chih-Lung Fu, Vee Y Tan, Joshua W McCausland, Daniel E Sturdevant, Hwang-Soo Joo, Shu Y Queck, Gordon Y C Cheung, Michael Otto
Bacterial sepsis is a major killer in hospitalized patients. Coagulase-negative staphylococci (CNS) with the leading species Staphylococcus epidermidis are the most frequent causes of nosocomial sepsis, with most infectious isolates being methicillin-resistant. However, which bacterial factors underlie the pathogenesis of CNS sepsis is unknown. While it has been commonly believed that invariant structures on the surface of CNS trigger sepsis by causing an over-reaction of the immune system, we show here that sepsis caused by methicillin-resistant S...
February 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28147885/bioactive-compounds-from-hypericum-humifusum-and-hypericum-perfoliatum-inhibition-potential-of-polyphenols-with-acetylcholinesterase-and-key-enzymes-linked-to-type-2-diabetes
#19
Afef Béjaoui, Issam Ben Salem, Nesrine Rokbeni, Yassine M'rabet, Mohamed Boussaid, Abdennacer Boulila
CONTEXT: Natural products are reported to have a wide spectrum of pharmacological properties such as antimicrobial, anti-inflammatory and anti-cholinesterase. The genus Hypericum (Hypericaceae) is a source of a variety of molecules with different biological activities, notably hypericin and various phenolics. OBJECTIVES: The goals of the present work were the determination of total phenolic and flavonoid content, hypericin and hyperforin concentration as well as the evaluation of biological of Hypericum humifusum L...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28145953/vancomycin-and-ceftazidime-in-bone-cement-as-a-potentially-effective-treatment-for-knee-periprosthetic-joint-infection
#20
Yung-Heng Hsu, Chih-Chien Hu, Pang-Hsin Hsieh, Hsin-Nung Shih, Steve W N Ueng, Yuhan Chang
BACKGROUND: The aim of this study was to determine the optimal formulation of antibiotic-loaded bone cement for knee periprosthetic joint infection. We used both in vitro and in vivo models incorporating various broad-spectrum antibiotics and tested their efficacy against gram-positive and gram-negative bacteria. METHODS: Bone cement specimens loaded with 4 g of either vancomycin or teicoplanin and 4 g of ceftazidime, imipenem, or aztreonam were studied to measure their in vitro antibiotic release characteristics and antibacterial capacities against methicillin-susceptible Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli...
February 1, 2017: Journal of Bone and Joint Surgery. American Volume
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