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Staphylococcus epidermidis

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https://www.readbyqxmd.com/read/28717325/tetracycline-resistance-phenotypes-and-genotypes-of-coagulase-negative-staphylococcal-isolates-from-bubaline-mastitis-in-egypt
#1
K A Abd El-Razik, A A Arafa, R H Hedia, E S Ibrahim
AIM: This study was devoted to elucidate the tetracycline resistance of coagulase-negative staphylococci (CNS) derived from normal and subclinical mastitic (SCM) buffaloes' milk in Egypt. MATERIALS AND METHODS: A total of 81 milk samples from 46 normal buffalo milk samples and 35 SCM buffalo milk samples at private dairy farms of Egypt were used in this study. CNS were identified using phenotypic and molecular methods (polymerase chain reaction [PCR]). CNS isolates were tested for tetracycline resistance using routine methods and multiplex PCR targeting tetracycline (tet) resistance genes followed by sequencing of positive PCR products and phylogenetic analysis...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28715352/commensal-bacterial-modulation-of-the-host-immune-response-to-ameliorate-pain-in-a-murine-model-of-chronic-prostatitis
#2
Stephen F Murphy, Anthony J Schaeffer, Joseph D Done, Marsha L Quick, Utkucan Acar, Praveen Thumbikat
The human commensal microflora plays an essential role in modulating the immune response to control homeostasis. Staphylococcus epidermidis, a commensal bacterium most commonly associated with the skin exerts such effects locally, modulating local immune responses during inflammation and preventing superinfection by pathogens such as Staphylococcus aureus. Although the prostate is considered by many to be sterile, multiple investigations have shown that small numbers of gram-positive bacterial species such as S...
August 2017: Pain
https://www.readbyqxmd.com/read/28714327/elevated-levels-of-circulating-cell-free-dna-and-neutrophil-proteins-are-associated-with-neonatal-sepsis-and-necrotizing-enterocolitis-in-immature-mice-pigs-and-infants
#3
Duc Ninh Nguyen, Allan Stensballe, Jacqueline Cy Lai, Pingping Jiang, Anders Brunse, Yanqi Li, Jing Sun, Carina Mallard, Tom Skeath, Nicholas D Embleton, Janet E Berrington, Per T Sangild
Preterm infants are highly susceptible to late-onset sepsis (LOS) and necrotizing enterocolitis (NEC), but disease pathogenesis and specific diagnostic markers are lacking. Circulating cell-free DNA (cfDNA) and immune cell-derived proteins are involved in multiple immune diseases in adults but have not been investigated in preterm neonates. We explored the relation of circulating neutrophil-associated proteins and cfDNA to LOS and/or NEC. Using a clinically relevant preterm pig model of spontaneous LOS and NEC development, we investigated neutrophil-associated proteins and cfDNA in plasma, together with cytokines in gut tissues...
January 1, 2017: Innate Immunity
https://www.readbyqxmd.com/read/28714053/the-influence-of-surface-texture-and-wettability-on-initial-bacterial-adhesion-on-titanium-and-zirconium-oxide-dental-implants
#4
Torsten Wassmann, Stefan Kreis, Michael Behr, Ralf Buergers
BACKGROUND: This study aims to investigate bacterial adhesion on different titanium and ceramic implant surfaces, to correlate these findings with surface roughness and surface hydrophobicity, and to define the predominant factor for bacterial adhesion for each material. METHODS: Zirconia and titanium specimens with different surface textures and wettability (5.0 mm in diameter, 1.0 mm in height) were prepared. Surface roughness was measured by perthometer (R a ) and atomic force microscopy, and hydrophobicity according to contact angles by computerized image analysis...
December 2017: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/28713777/a-novel-chimeric-endolysin-with-antibacterial-activity-against-methicillin-resistant-staphylococcus-aureus
#5
Hamed Haddad Kashani, Hossein Fahimi, Yasaman Dasteh Goli, Rezvan Moniri
Cysteine/histidine-dependent amidohydrolase/peptidase (CHAP) and amidase are known as catalytic domains of the bacteriophage-derived endolysin LysK and were previously reported to show lytic activity against methicillin-resistant Staphylococcus aureus (MRSA). In the current study, the in silico design and analysis of chimeric CHAP-amidase model was applied to enhance the stability and solubility of protein, which was achieved through improving the properties of primary, secondary and tertiary structures. The coding gene sequence of the chimeric CHAP-amidase was synthesized and subcloned into the pET-22(+) expression vector, and the recombinant protein was expressed in E...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28712577/bacterial-adherence-to-graft-tissues-in-static-and-flow-conditions
#6
Tiago Rafael Veloso, Jorien Claes, Soetkin Van Kerckhoven, Bartosz Ditkowski, Luis G Hurtado-Aguilar, Stefan Jockenhoevel, Petra Mela, Ramadan Jashari, Marc Gewillig, Marc F Hoylaerts, Bart Meyns, Ruth Heying
BACKGROUND: Various conduits and stent-mounted valves are used as pulmonary valve graft tissues for right ventricular outflow tract reconstruction with good hemodynamic results. Valve replacement carries an increased risk of infective endocarditis (IE). Recent observations have increased awareness of the risk of IE after transcatheter implantation of a stent-mounted bovine jugular vein valve. This study focused on the susceptibility of graft tissue surfaces to bacterial adherence as a potential risk factor for subsequent IE...
June 16, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28712270/folliculitis-caused-by-staphylococcus-epidermidis
#7
Stefano Veraldi, Cinzia Desimine, Anna Grancini
No abstract text is available yet for this article.
July 13, 2017: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
https://www.readbyqxmd.com/read/28712037/introduction-of-sample-tubes-with-sodium-azide-as-a-preservative-for-ethyl-glucuronide-in-urine
#8
Marc Luginbühl, Wolfgang Weinmann, Ali Al-Ahmad
Ethyl glucuronide (EtG) is a direct alcohol marker, which is widely used for clinical and forensic applications, mainly for abstinence control. However, the instability of EtG in urine against bacterial degradation or the post-collectional synthesis of EtG in contaminated samples may cause false interpretation of EtG results in urine samples. This study evaluates the potential of sodium azide in tubes used for urine collection to hinder degradation of ethyl glucuronide by bacterial metabolism taking place during growth of bacterial colonies...
July 15, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28711311/bacterial-colonization-of-penile-prosthesis-after-its-withdrawal-due-to-mechanical-failure
#9
B Etcheverry-Giadrosich, J Torremadé-Barreda, L Pujol-Galarza, F Vigués-Julià
INTRODUCTION: Prosthetic surgery to treat erectile dysfunction has a risk of infection of up to 3%, but this risk can increase to 18% when the surgery involves replacement. This increased risk of infection is attributed to the bacterial colonization of the prosthesis during the initial surgery. OBJECTIVE: To analyse the presence of germs in the prosthesis that is withdrawn due to mechanical failure (not infection), as well as the surgical results and its progression...
July 12, 2017: Actas Urologicas Españolas
https://www.readbyqxmd.com/read/28707817/evaluation-of-free-or-anchored-antimicrobial-peptides-as-candidates-for-the-prevention-of-orthopaedic-device-related-infections
#10
Francesca D'Este, Debora Oro, Gerard Boix-Lemonche, Alessandro Tossi, Barbara Skerlavaj
The prevention of implant-associated infection, one the most feared complications in orthopaedic surgery, remains a major clinical challenge and urges development of effective methods to prevent bacterial colonization of implanted devices. Alpha-helical antimicrobial peptides (AMPs) may be promising candidates in this respect due to their potent and broad-spectrum antimicrobial activity, their low tendency to elicit resistance and possible retention of efficacy in the immobilized state. The aim of this study was to evaluate the potential of five different helical AMPs, the cathelicidins BMAP-27 and BMAP-28, their (1-18) fragments and the rationally designed, artificial P19(9/G7) peptide, for the prevention of orthopaedic implant infections...
July 14, 2017: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/28707417/defining-the-metal-specificity-of-a-multifunctional-biofilm-adhesion-protein
#11
Catherine T Chaton, Andrew B Herr
The accumulation associated protein (Aap) of Staphylococcus epidermidis mediates intercellular adhesion events necessary for biofilm growth. This process depends upon Zn(2+) -induced self-assembly of G5 domains within the B-repeat region of the protein, forming anti-parallel, intertwined protein 'ropes' between cells. Pleomorphism in the Zn(2+) -coordinating residues was observed in previously solved crystal structures, suggesting that the metal binding site might accommodate other transition metals and thereby support dimerization...
July 13, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28705673/global-acquisition-of-genetic-materials-from-different-bacteria-into-the-staphylococcal-cassette-chromosome-elements-of-a-staphylococcus-epidermidis
#12
Huping Xue, Zhaowei Wu, Dandan Qiao, Chao Tong, Xin Zhao
Staphylococcus epidermidis has been suggested as a main reservoir of methicillin-resistant and virulence genes to facilitate evolution of Staphylococcus aureus as a successful pathogen. However, it remains a mystery where and how S. epidermidis obtains numerous genes to serve as the reservoir. A methicillin-resistant S. epidermidis isolate NW32 from a mastitic milk sample was sequenced and its staphylococcal cassette chromosome (SCC) elements were characterized in this study. The SCC composite island covered 3...
July 10, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28704823/different-behavior-and-response-of-staphylococcus-epidermidis-and-streptococcus-pneumoniae-to-a-ventriculoperitoneal-shunt-an-in-vitro-study
#13
Vahid Heidari, Zohreh Habibi, Ahmadreza Hojjati Marvasti, Zahra Ebrahim Soltani, Negin Naderian, Parvin Tanzifi, Farideh Nejat
BACKGROUND: Pneumococcal shunt infection is a rare event. There is no consensus on the therapeutic management of this kind of shunt infection according to literature reviews, and it seems to be different from infection with Staphylococcus epidermidis. We studied 2 shunted patients with pneumococcal meningitis, both of whom were treated with only antibiotics. The management of these cases seems to be different from that of shunt catheter infection due to these bacteria. We conducted a laboratory study to show the different behavior of pneumococcus compared to S...
July 14, 2017: Pediatric Neurosurgery
https://www.readbyqxmd.com/read/28704596/silver-nanoparticles-functionalized-with-ampicillin
#14
Agnieszka Rogowska, Katarzyna Rafińska, Paweł Pomastowski, Justyna Walczak, Viorica Railean-Plugaru, Magdalena Buszewska-Forajta, Bogusław Buszewski
The resistance of pathogenic bacteria to antibiotics has become a serious problem. The emphasis is placed on the development of new, effective antimicrobial strategies. One of them is the use of AgNPs in association with antibiotic drugs. The aim of this study was to obtain silver nanoparticles functionalized with ampicillin and to investigate the mechanism of binding antibiotics to nanoparticle using high-performance liquid chromatography approach. To confirm the occurrence of silver nanoparticles functionalization, FTIR, MALDI-TOF MS, and DLS analysis and zeta potential measurements were performed...
July 13, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28702199/prevalence-of-methicillin-resistance-and-macrolide-lincosamide-streptogramin-b-resistance-in-staphylococcus-haemolyticus-among-clinical-strains-at-a-tertiary-care-hospital-in-thailand
#15
S Teeraputon, P Santanirand, T Wongchai, W Songjang, N Lapsomthob, D Jaikrasun, S Toonkaew, P Tophon
Staphylococcus spp. is a major cause of nosocomial infection and sepsis. However, increasing drug resistance is becoming a challenge to microbiologists. The purpose of this study was to identify and determine antimicrobial resistance phenotypes and drug resistance genes of clinical coagulase-negative staphylococci (CoNS) isolates at Mae Sot Hospital in Tak province, Thailand. A total of 229 CoNS isolates were collected from clinical specimens during two periods in 2014 and in 2015. Staphylococcus haemolyticus was the most prevalent species (37...
September 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/28699258/cytotoxic-peptides-with-insulin-releasing-activities-from-skin-secretions-of-the-italian-stream-frog-rana-italica-ranidae
#16
J Michael Conlon, Vishal Musale, Samir Attoub, Maria Luisa Mangoni, Jérôme Leprince, Laurent Coquet, Thierry Jouenne, Yasser H A Abdel-Wahab, Peter R Flatt, Andrea C Rinaldi
Peptidomic analysis of norepinephrine-stimulated skin secretions from Italian stream frog Rana italica led to the purification and characterization of two host-defense peptides differing by a single amino acid residue belonging to the brevinin-1 family (brevinin-1ITa and -1ITb), a peptide belonging to the temporin family (temporin-ITa) and a component identified as prokineticin Bv8. The secretions contained relatively high concentrations of the methionine-sulphoxide forms of brevinin-1ITa and -1ITb suggesting that these peptides may have a role as antioxidants in the skin of this montane frog...
July 11, 2017: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/28699251/antituberculotic-activity-of-actinobacteria-isolated-from-the-rare-habitats
#17
Aehtesham Hussain, Muzafar Ahmad Rather, Aabid Manzoor Shah, Zubair Shanib, Aiyatullah Shah, Zahoor Ahmad, Qazi Parvaiz Hassan
A distinctive screening procedure resulted in the isolation and identification of antituberculotic actinobacteria. In this course, a total of 125 actinobacteria were isolated from various soil samples from untapped areas in Northwestern Himalayas, India. The antibacterial screening showed that 26 isolates inhibited the growth of at least one of the tested bacterial pathogens including Staphylococcus aureus (ATCC 25923), Staphylococcus epidermidis (ATCC 12228), Bacillus subtilis (ATCC 11774), Micrococcus luteus (ATCC 10240), Escherichia coli (10536), Pseudomonas aeruginosa (ATCC 10145) and Klebsiella pneumonia (ATCC BAA-2146)...
July 12, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28698913/antibacterial-and-antioxidant-constituents-of-extracts-of-endophytic-fungi-isolated-from-ocimum-basilicum-var-thyrsiflora-leaves
#18
Piyamas Atiphasaworn, Sakon Monggoot, Eleni Gentekaki, Siraprapa Brooks, Patcharee Pripdeevech
Fourteen fungal endophytes were isolated from the Ocimum basilicum var. thyrsiflora leaves collected from Northern Thailand. Eight genera were identified including Aspergillus, Ascochyta, Nigrospora, Blastomyces, Colletotrichum, Exidia, Clitopilus, and Nomuraea. The antibacterial activity of crude extracts from all endophytic fungi was tested against nine human bacterial pathogens: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Vibrio cholerae, and Vibrio parahaemolyticus...
July 12, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28698836/copper-as-an-alternative-antimicrobial-coating-for-implants-an-in-vitro-study
#19
Claudia Bergemann, Sarah Zaatreh, Katharina Wegner, Kathleen Arndt, Andreas Podbielski, Rainer Bader, Cornelia Prinz, Ulrich Lembke, J Barbara Nebe
AIM: To investigate osteoconductive and antimicrobial properties of a titanium-copper-nitride (TiCuN) film and an additional BONIT(®) coating on titanium substrates. METHODS: For micro-structuring, the surface of titanium test samples was modified by titanium plasma spray (TPS). On the TPS-coated samples, the TiCuN layer was deposited by physical vapor deposition. The BONIT(®) layer was coated electrochemically. The concentration of copper ions released from TiCuN films was measured by atomic absorption spectrometry...
June 24, 2017: World Journal of Transplantation
https://www.readbyqxmd.com/read/28698511/the-effect-of-silver-nanoparticles-size-produced-using-plant-extract-from-arbutus-unedo-on-their-antibacterial-efficacy
#20
Nicholas Skandalis, Anastasia Dimopoulou, Anthie Georgopoulou, Nikolaos Gallios, Dimitrios Papadopoulos, Dimitrios Tsipas, Ioannis Theologidis, Nikolaos Michailidis, Maria Chatzinikolaidou
Silver nanoparticles (AgNPs) have been demonstrated to restrain bacterial growth, while maintaining minimal risk in development of bacterial resistance and human cell toxicity that conventional silver compounds exhibit. Several physical and chemical methods have been reported to synthesize AgNPs. However, these methods are expensive and involve heavy chemical reduction agents. An alternative approach to produce AgNPs in a cost-effective and environmentally friendly way employs a biological pathway using various plant extracts to reduce metal ions...
July 10, 2017: Nanomaterials
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