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Staphylococcus epidermidis

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https://www.readbyqxmd.com/read/28542390/analysis-of-early-mesothelial-cell-responses-to-staphylococcus-epidermidis-isolated-from-patients-with-peritoneal-dialysis-associated-peritonitis
#1
Amanda L McGuire, Kieran T Mulroney, Christine F Carson, Ramesh Ram, Grant Morahan, Aron Chakera
The major complication of peritoneal dialysis (PD) is the development of peritonitis, an infection within the abdominal cavity, primarily caused by bacteria. PD peritonitis is associated with significant morbidity, mortality and health care costs. Staphylococcus epidermidis is the most frequently isolated cause of PD-associated peritonitis. Mesothelial cells are integral to the host response to peritonitis, and subsequent clinical outcomes, yet the effects of infection on mesothelial cells are not well characterised...
2017: PloS One
https://www.readbyqxmd.com/read/28540152/epidemiological-clinical-and-microbiological-characteristics-of-patients-with-post-traumatic-osteomyelitis-of-limb-fractures-in-southwest-china-a-hospital-based-study
#2
Jiachen Peng, Youliang Ren, Wenbin He, Zhengdao Li, Jin Yang, Yi Liu, Zhonghui Zheng, Stephen L Kates, Edward M Schwarz, Chao Xie, Youjia Xu
Objective To determine the epidemiological, clinical and microbiological characteristics, of patients with post-traumatic osteomyelitis of extremity fractures, and provide evidence-based guidelines for early diagnosis and treatment, including empiric antibiotic therapy. Methods Human subject research was performed using institutional review board approved protocols. A retrospective chart review was conducted on 5,368 patients diagnosed with extremity traumatic fractures from January 1, 2012 to December 31, 2015, to identify osteomyelitis patients...
2017: J Bone Jt Infect
https://www.readbyqxmd.com/read/28538949/the-follicular-skin-microbiome-in-patients-with-hidradenitis-suppurativa-and-healthy-controls
#3
Hans Christian Ring, Jonathan Thorsen, Ditte M Saunte, Berit Lilje, Lene Bay, Peter Theut Riis, Niels Larsen, Lee O'Brien Andersen, Henrik V Nielsen, Iben M Miller, Thomas Bjarnsholt, Kurt Fuursted, Gregor Borut Jemec
Importance: Although the pathogenesis of hidradenitis suppurativa (HS) remains enigmatic, several factors point to potential involvement of the cutaneous microbiome. Insight into the cutaneous microbiome in HS using next-generation sequencing may provide novel data on the microbiological diversity of the skin. Objective: To investigate the follicular skin microbiome in patients with HS and in healthy controls. Design, Setting, and Participants: This case-control study obtained punch biopsy specimens from patients with HS (lesional and nonlesional) and healthy controls between October 1, 2014, and August 1, 2016...
May 24, 2017: JAMA Dermatology
https://www.readbyqxmd.com/read/28538851/-antimicrobial-sensitivity-of-the-environmental-microbiota-in-the-intensive-care-units-of-a-peruvian-hospital
#4
José Díaz-Tello, Jesús Rojas-Jaimes, Jimmy Ibarra-Trujillo, Delza Tárraga-Gonzales
The objective was to detect Gram-negative bacilli and Gram-positive cocci isolated from the environmental microbiota of the Intensive Care Unit (ICU) departments of Neonatology, Pediatrics, and Transplants (kidney, liver, and general) in a Lima hospital and determine their antimicrobial sensitivity. Eighty samples were obtained from inanimate surfaces using a wet swab. A total of 61 bacterial strains were identified, including Staphylococcus epidermis (46.0%), Alcaligenes sp. (21.3%), Pseudomonas aeruginosa (16...
January 2017: Revista Peruana de Medicina Experimental y Salud Pública
https://www.readbyqxmd.com/read/28538674/biosynthesis-of-zno-nanoparticles-by-a-new-pichia-kudriavzevii-yeast-strain-and-evaluation-of-their-antimicrobial-and-antioxidant-activities
#5
Amin Boroumand Moghaddam, Mona Moniri, Susan Azizi, Raha Abdul Rahim, Arbakariya Bin Ariff, Wan Zuhainis Saad, Farideh Namvar, Mohammad Navaderi
The potential ability of a new yeast strain, Pichia kudriavzevii, in the synthesis of zinc oxide nanoparticles (ZnO-NPs) through a green method was explored in this study. The effect of reaction time (12, 24 and 36 h) on the structure of the resulting ZnO nanoparticles was investigated. From the XRD and TEM results, the ZnO-NPs with a hexagonal wurtzite structure and a particle crystal size of ~10-61 nm was formed at different reaction times. Combing XRD, TEM, and PL results, it was revealed that the sample prepared at intermediate duration (24 h) has the most favorable nanosized structure with the lowest defect concentration...
May 24, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28537028/enhanced-larvicidal-antibacterial-and-photocatalytic-efficacy-of-tio2-nanohybrids-green-synthesized-using-the-aqueous-leaf-extract-of-parthenium-hysterophorus
#6
Keerthika Thandapani, Manikandan Kathiravan, Elangovan Namasivayam, Indira Arulselvi Padiksan, Geetha Natesan, Manish Tiwari, Benelli Giovanni, Venkatachalam Perumal
Titanium dioxide nanoparticles are emerging as a biocompatible nanomaterial with multipurpose bioactivities. In this study, titanium dioxide (TiO2) nanoparticles were effectively synthesized using the aqueous leaf extracts of Parthenium hysterophorus prepared by microwave irradiation. TiO2 nanoparticles were fabricated by treating the P. hysterophorus leaf extracts with the TiO4 solution. Biologically active compounds such as alcohols, phenols, alkanes, and fluoroalkanes were involved in bioreduction of TiO4 into TiO2...
May 23, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28532810/species-level-identification-of-coagulase-negative-staphylococcus-spp-from-buffalo-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-and-cydb-real-time-quantitative-pcr
#7
Lucas J L Pizauro, Camila C de Almeida, Glenn A Soltes, Durda Slavic, Oswaldo D Rossi-Junior, Fernando A de Ávila, Luiz F Zafalon, Janet I MacInnes
Incorrect identification of Staphylococcus spp. can have serious clinical and zoonotic repercussions. Accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and/or cydB real- time quantitative PCR (qPCR) could be used to accurately identify coagulase negative Staphylococcus spp. (CoNS) obtained from buffalo milk and milking environment samples. Seventy-five of 84 CoNS isolates could be identified to the species level (score value >1...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28532030/tetracycline-hydrochloride-loaded-electrospun-nanofibers-mats-based-on-pva-and-chitosan-for-wound-dressing
#8
Alex Carvalho Alavarse, Fernanda Waitman de Oliveira Silva, Jandir Telleria Colque, Viviam Moura da Silva, Tatiane Prieto, Everaldo Carlos Venancio, Jean-Jacques Bonvent
Fibrous mats built from biopolymer have been extensively explored for tissue engineering due mainly to their mimic structure to the extracellular matrix. The incorporation of drug in such scaffolds represents a growing interest for control drug delivery system in order to promote the tissue repair. In the present work, we present an experimental investigation of morphological, thermal, mechanical, drug release, antibacterial and cytotoxicity properties of electrospun PVA/Chitosan and PVA/Chitosan/Tetracycline hydrochloride (TCH) mats for wound dressing...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531880/controlled-release-of-moxifloxacin-from-intraocular-lenses-modified-by-ar-plasma-assisted-grafting-with-amps-or-sbma-an-in-vitro-study
#9
A F R Pimenta, A P Vieira, R Colaço, B Saramago, M H Gil, P Coimbra, P Alves, D Bozukova, T R Correia, I J Correia, A J Guiomar, A P Serro
Intraocular lenses (IOLs) present an alternative for extended, local drug delivery in the prevention of post-operative acute endophthalmitis. In the present work, we modified the surface of a hydrophilic acrylic material, used for manufacturing of IOLs, through plasma-assisted grafting copolymerization of 2-acrylamido-2-methylpropane sulfonic acid (AMPS) or [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (SBMA), with the aim of achieving a controlled and effective drug release. The material was loaded with moxifloxacin (MFX), a commonly used antibiotic for endophthalmitis prevention...
May 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28531875/elaboration-of-antibacterial-plastic-surfaces-by-a-combination-of-antiadhesive-and-biocidal-coatings-of-natural-products
#10
Jean-Baptiste Paris, Damien Seyer, Thierry Jouenne, Pascal Thébault
Antibacterial polyolefins surfaces, combining biocidal and antiadhesive properties, were elaborated by a covalent grafting of antimicrobial peptides (AMPs), able to kill adherent bacteria, on a pre-immobilized hyaluronic acid (HA) layer, able to repel the micro-organisms. Different HA activation rate for its immobilization, were used to change HA layer morphology and number of residual free carboxylic acid functions for AMPs grafting. Based on adhesion tests on Staphylococcus epidermidis and microscopy fluorescent observations, the presence of the two combined properties was shown to be depended on the HA activation rate...
May 13, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28527793/prevalence-of-non-aureus-staphylococcus-species-causing-intramammary-infections-in-canadian-dairy-herds
#11
Larissa A Z Condas, Jeroen De Buck, Diego B Nobrega, Domonique A Carson, Sohail Naushad, Sarne De Vliegher, Ruth N Zadoks, John R Middleton, Simon Dufour, John P Kastelic, Herman W Barkema
Non-aureus staphylococci (NAS), the microorganisms most frequently isolated from bovine milk worldwide, are a heterogeneous group of numerous species. To establish their importance as a group, the distribution of individual species needs to be determined. In the present study, NAS intramammary infection (IMI) was defined as a milk sample containing ≥1,000 cfu/mL in pure or mixed culture that was obtained from a cohort of cows assembled by the Canadian Bovine Mastitis Research Network. Overall, 6,213 (6.3%) of 98,233 quarter-milk samples from 5,149 cows and 20,305 udder quarters were associated with an NAS IMI...
May 17, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28524724/antibacterial-effect-of-hydrogen-peroxide-titanium-dioxide-suspensions-in-the-decontamination-of-rough-titanium-surfaces
#12
David Wiedmer, Fernanda Cristina Petersen, Jessica Lönn-Stensrud, Hanna Tiainen
The chemical decontamination of infected dental implants is essential for the successful treatment of peri-implantitis. The aim of this study was to assess the antibacterial effect of a hydrogen peroxide-titanium dioxide (H2O2-TiO2) suspension against Staphylococcus epidermidis biofilms. Titanium (Ti) coins were inoculated with a bioluminescent S. epidermidis strain for 8 h and subsequently exposed to H2O2 with and without TiO2 nanoparticles or chlorhexidine (CHX). Bacterial regrowth, bacterial load and viability after decontamination were analyzed by continuous luminescence monitoring, live/dead staining and scanning electron microscopy...
May 19, 2017: Biofouling
https://www.readbyqxmd.com/read/28524087/in-vitro-assessment-of-early-bacterial-activity-on-micro-nanostructured-ti6al4v-surfaces
#13
Benjamin Valdez-Salas, Ernesto Beltrán-Partida, Sandra Castillo-Uribe, Mario Curiel-Álvarez, Roumen Zlatev, Margarita Stoytcheva, Gisela Montero-Alpírez, Lidia Vargas-Osuna
It is imperative to understand and systematically compare the initial interactions between bacteria genre and surface properties. Thus, we fabricated a flat, anodized with 80 nm TiO₂ nanotubes (NTs), and a rough Ti6Al4V surface. The materials were characterized using field-emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM). We cultured in vitro Staphylococcus epidermidis (S. epidermidis) and Pseudomonas aeruginosa (P. aeruginosa) to evaluate the bacterial-surface behavior by FE-SEM and viability calculation...
May 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28522724/draft-genome-sequence-of-staphylococcus-epidermidis-clinical-strain-goi1153754-03-14-isolated-from-an-infected-knee-prosthesis
#14
Marta Bottagisio, Alessio Soggiu, Arianna B Lovati, Marco Toscano, Cristian Piras, Carlo L Romanò, Luigi Bonizzi, Paola Roncada, Lorenzo Drago
We announce the draft genome sequence of Staphylococcus epidermidis clinical strain GOI1153754-03-14, isolated from an infected orthopedic prosthesis. The reported genomic sequence will provide valuable information concerning the mechanisms of the biofilm formation on metallic implants.
May 18, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28522446/non-contact-electromagnetic-induction-heating-for-eradicating-bacteria-and-yeasts-on-biomaterials-and-possible-relevance-to-orthopaedic-implant-infections-in-vitro-findings
#15
B G Pijls, I M J G Sanders, E J Kuijper, R G H H Nelissen
OBJECTIVES: Infection of implants is a major problem in elective and trauma surgery. Heating is an effective way to reduce the bacterial load in food preparation, and studies on hyperthermia treatment for cancer have shown that it is possible to heat metal objects with pulsed electromagnetic fields selectively (PEMF), also known as induction heating. We therefore set out to answer the following research question: is non-contact induction heating of metallic implants effective in reducing bacterial load in vitro? METHODS: Titanium alloy cylinders (Ti6Al4V) were exposed to PEMF from an induction heater with maximum 2000 watts at 27 kHz after being contaminated with five different types of micro-organisms: Staphylococcus epidermidis; Staphylococcus aureus; Pseudomonas aeruginosa; spore-forming Bacillus cereus; and yeast Candida albicans...
May 2017: Bone & Joint Research
https://www.readbyqxmd.com/read/28506885/high-proportions-of-staphylococcus-epidermidis-in-dental-caries-harbor-multiple-classes-of-antibiotics-resistance-significantly-increase-inflammatory-interleukins-in-dental-pulps
#16
Darshan Devang Divakar, Muzaheed, Sultan Salem Aldeyab, Sara A Alfawaz, Abdulaziz Abdullah AlKheraif, Aftab Ahmed Khan
Staphylococcus epidermidis is one of most prevalent in dental caries or dental pulp which has the capability of horizontal genetic transfer between different bacterial species in the oropharynx, suggesting that it may evolve with the dissemination of resistant determinants, This study was performed to molecularly characterize and differentiate S. epidermidis isolated from dental caries and healthy individual. Also, two important cytokines in inflammation were assayed caused due to S. epidermidis of health and dental caries sources...
May 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28504348/broad-spectrum-macromolecular-antimicrobials-with-biofilm-disruption-capability-and-in-vivo-efficacy
#17
Jeremy P K Tan, Daniel J Coady, Haritz Sardon, Alexander Yuen, Shujun Gao, Shaun W Lim, Zhen Chang Liang, Eddy W Tan, Shrinivas Venkataraman, Amanda C Engler, Mareva Fevre, Robert Ono, Yi Yan Yang, James L Hedrick
In this study, antimicrobial polymers are synthesized by the organocatalytic ring-opening polymerization of an eight-membered heterocyclic carbonate monomer that is subsequently quaternized with methyl iodide. These polymers demonstrate activity against clinically relevant Gram-positive Staphylococcus epidermidis and Staphylococcus aureus, Gram-negative Escherichia coli and Pseudomonas aeruginosa, and fungus Candida albicans with fast killing kinetics. Importantly, the polymer efficiently inhibits biofilm growth and lyses existing biofilm, leading to a reduction in biomass and cell viability...
May 15, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28502956/evaluation-of-biofilm-forming-ability-of-bacterial-strains-isolated-from-the-roof-of-an-old-house
#18
Marwa Jardak, Faten Abdelli, Rahma Laadhar, Raphael Lami, Didier Stien, Sami Aifa, Sami Mnif
The bacterial diversity associated with biofilm-forming ability was studied. Eighteen bacterial strains were isolated from a microbial film collected from the roof of an old house located in Sfax, Tunisia. The purity of these microorganisms was confirmed by microscopic observation after repeated streaking on a Tryptic Soy agar medium. Biofilm formation was estimated using preliminary tests including a motility test, microbial adhesion to solvents (MATS), and the Congo Red Agar method (CRA). Since these tests showed no significant result, microplate tests, such as crystal violet and resazurin assays, were used...
May 15, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28496492/infective-endocarditis-report-from-a-main-referral-teaching-hospital-in-iran
#19
Behrooz Heydari, Iman Karimzadeh, Hossein Khalili, Esfandiar Shojaei, Abdolrasool Ebrahimi
BACKGROUND/OBJECTIVE: The aim of the present preliminary study was to assess the demographic, clinical, paraclinical, microbiological, echocardiographic, and therapeutic profile as well as in-hospital outcome of patients with infective endocarditis at a referral center for various infectious diseases in Iran. METHODS: Required demographic, clinical, plausible complications and paraclinical data were collected from patients' medical charts. Echocardiographic findings were obtained by performing transthoracic and/or transesophageal echocardiography as clinically indicated...
2017: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/28491057/identification-of-genes-controlled-by-the-essential-yycfg-two-component-system-reveals-a-role-for-biofilm-modulation-in-staphylococcus-epidermidis
#20
Tao Xu, Yang Wu, Zhiwei Lin, Ralph Bertram, Friedrich Götz, Ying Zhang, Di Qu
Biofilms play a crucial role in the pathogenicity of Staphylococcus epidermidis, while little is known about whether the essential YycFG two-component signal transduction system (TCS) is involved in biofilm formation. We used antisense RNA (asRNA) to silence the yycFG TCS in order to study its regulatory functions in S. epidermidis. Strain 1457 expressing asRNA yycF exhibited a significant delay (~4-5 h) in entry to log phase, which was partially complemented by overexpressing ssaA. The expression of asRNA yycF and asRNA yycG resulted in a 68 and 50% decrease in biofilm formation at 6 h, respectively, while they had no significant inhibitory effect on 12 h biofilm formation...
2017: Frontiers in Microbiology
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