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Staphylococcus epidermidis

Francesco Boschetto, Nami Toyama, Satoshi Horiguchi, Ryan M Bock, Bryan J McEntire, Tetsuya Adachi, Elia Marin, Wenliang Zhu, Osam Mazda, B Sonny Bal, Giuseppe Pezzotti
The metabolic response of Gram-positive Staphylococcus epidermidis (S. epidermidis) bacteria to bioceramic substrates was probed by means of Fourier transform infrared spectroscopy (FTIR). Oxide zirconia-toughened alumina (ZTA) and non-oxide silicon nitride (Si3N4) substrates were tested. Bacteria exposed to silica glass substrates were used as a control. S. epidermidis, a major cause of periprosthetic infections, was screened to obtain a precise time-lapse knowledge of its molecular composition and to mechanistically understand its interaction with different substrates...
April 18, 2018: Analyst
Abeer Mostafa Abdalhamed, Gamil Sayed Gamil Zeedan, Hala Abdoula Ahmed Abou Zeina
Aim: The present work aims to isolate and identify bacteria that cause mastitis in small ruminants and evaluates the antibacterial activity of some antibiotics, honey, essential oils, and plant extracts. Materials and Methods: A total of 289 milk samples were collected from udder secretions of sheep (n=189) and goat (n=100) from El-Fayoum, Beni-Suef, and Giza governorates. Screening subclinical mastitis (SCM) was done using California Mastitis Test (CMT); identification of the isolates was achieved using Gram's staining, hemolytic pattern, colony morphology, and biochemical tests using Analytical Profile Index...
March 2018: Veterinary World
J M Bello-López, L Delgado-Balbuena, D Rojas-Huidobro, J Rojo-Medina
OBJECTIVES: Transfusion of hemocomponents is essential for clinical and surgical procedures and therefore their safety has increased. An option for pathogen reduction includes the combination of riboflavin and UV light. To our knowledge, there are no studies in Latin America that demonstrate the effectiveness of the pathogen reduction in hemocomponents. The objective of this work was to evaluate the efficiency of a pathogens reduction system in platelets concentrates (PC) and plasma. MATERIALS AND METHODS: PC and plasma were contaminated with Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes and Staphylococcus epidermidis at 104 to 106  CFU and subjected to bacterial reduction...
April 12, 2018: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
Beatriz Alonso, María Jesús Pérez-Granda, Ana Rodríguez-Huerta, Carmen Rodríguez, Emilio Bouza, María Guembe
Ethanol-based lock therapy (LT) solutions are used as an alternative to antibiotics for the conservative management of catheter-related bloodstream infection. However, no clear consensus on regimen or dose has been reached. Our objective was to find the ethanol-based lock solution containing a sufficiently low concentration of ethanol for reduction of the metabolic activity of bacterial and fungal biofilms. Using an in vitro model, we tested 3 concentrations of ethanol (25%-40%-70%), with and without 60 IU of heparin, at 6 different time points and against 24-hour preformed biofilms of Staphylococcus aureus ATCC29213, Staphylococcus epidermidis (clinical isolate), Enterococcus faecalis ATCC33186, Candida albicans ATCC14058, and Escherichia coli ATCC25922...
April 10, 2018: Journal of Hospital Infection
Unisha Patel, Kishor Chauhan, Shilpa Gupte
In the present work, magnetic nanoparticles (MNPs) were prepared by chemical precipitation of trivalent and divalent iron ions which were functionalized using citric acid. The bacterial isolate Staphylococcus epidermidis KX781317 was isolated from oil-contaminated site. The isolate produced lipase, which was purified and immobilized on magnetic nanoparticles (MNPs) for ester synthesis from waste frying oil (WFO). The characterization of MNPs employed conventional TEM, XRD and FTIR techniques. TEM analysis of MNPs showed the particle size in the range of 20-50 nm...
April 2018: 3 Biotech
Josiane Alhage, Hoda Elbitar, Samir Taha, Jean-Paul Guegan, Zeina Dassouki, Thomas Vives, Thierry Benvegnu
A phenylethanoid, two steroids, a flavone glucoside and a chalcone have been isolated for the first time from the stems of Calicotome villosa together with a previously isolated flavone glucoside. Their structures were determined by spectroscopic analyses (NMR, HRMS) as basalethanoïd B ( 1 ), β-sitosterol and stigmasterol ( 2 ), chrysine-7- O -β-d-glucopyranoside ( 3 ), chrysine 7-((6''- O -acetyl)- O -β-d-glucopyranoside) ( 4 ) and calythropsin ( 5 ). The crude extracts and the isolated compounds (except 4), were evaluated for their antioxidant, antimicrobial (against two Gram-positive bacterial strains: Staphylococcus aureus , Bacillus cereus , four Gram-negative bacterial strains: Staphylococcus epidermidis , Klebsiella pneumonia , Acinetobacter baumanii , and three yeasts: Candida albicans , Candida tropicalis , and Candida glabrata ), hemolytic, antidiabetic, anti-inflammatory and cytotoxic activity...
April 8, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Mélanie Dumont, Régis Villet, Manon Guirand, Alexandra Montembault, Thierry Delair, Stéphane Lack, Michel Barikosky, Agnès Crepet, Pierre Alcouffe, Frédéric Laurent, Laurent David
The preparation of chitosan-coated alginate fibers by a wet spin process is presented and the characterization of the antibacterial activities of these fibers is discussed. Preformed calcium alginate fibers were passed in chitosan acetate solutions. The coagulation method of the coating consisted in the immersion of fibers in a bath of calcium dihydroxide solution (0.1 M). The antibacterial evaluation was achieved by a CFU (Colony-Forming Units) counting method after 6 h of incubation at 37 °C. The incorporation of chitosan on calcium alginate fibers brings antibacterial activities against Staphylococcus epidermidis, Escherichia coli and various Staphylococcus aureus strains namely MSSA (Methicillin Sensitive Staphylococcus aureus), CA-MRSA (Community Associated Methicillin Resistant Staphylococcus aureus) and HA-MRSA (Healthcare Associated Methicillin Resistant Staphylococcus aureus) which make these chitosan-coated fibers potential candidates for wound dressing materials...
June 15, 2018: Carbohydrate Polymers
Ashok Rajgopal, Inayat Panda, Arun Rao, Vivek Dahiya, Himanshu Gupta
BACKGROUND: There has been a debate regarding the outcomes of 2-stage revision total knee arthroplasty (TKA) when debridement, antibiotics, irrigation, and implant retention (DAIR) had been carried out previously. The purpose of this study is to evaluate the influence of prior failed DAIR (F-DAIR) on the outcomes of 2-stage revision TKA done for periprosthetic joint infection (PJI). METHODS: This is a retrospective study of a consecutive series of 184 knees that completed 2-stage revision TKA for PJI, operated by a single surgeon between January 2000 and July 2011...
March 9, 2018: Journal of Arthroplasty
Yu Jie Lu, Takashi Sasaki, Kyoko Kuwahara-Arai, Yuki Uehara, Keiichi Hiramatsu
The nasal carriage rate of Staphylococcus aureus in human is 25--30% and S. aureus sporadically causes severe infections. However, mechanisms underlying staphylococcal carriage remain largely unknown. In the present study, we constructed an rpoB -based microbiome method for staphylococcal species discrimination. Based on a microbiome scheme targeting viable cell DNA using PMA dye (PMA microbiome), we also developed a new method to allow the comprehensive viability analysis of any bacterial taxon. To clarify the ecological distribution of staphylococci in the nasal microbiota, we applied these methods in 46 nasal specimens from healthy adults...
April 6, 2018: Applied and Environmental Microbiology
Shubhangi Bhende, Daniel Burkley, Jesse Nawrocki
BACKGROUND: This study evaluated the in vitro and in vivo anti-bacterial efficacy of STRATAFIX™ Symmetric PDS™ Plus Knotless Tissue Control Devices in comparison with standard absorbable polydioxanone devices lacking triclosan, utilizing challenges by gram-positive and gram-negative bacteria. METHODS: The STRATAFIX™ Symmetric PDS™ Plus Knotless Tissue Control Device was tested for in vitro efficacy against Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), S...
April 6, 2018: Surgical Infections
Margarita Trobos, Martin Lars Johansson, Sofia Jonhede, Hanna Peters, Maria Hoffman, Omar Omar, Peter Thomsen, Malou Hultcrantz
PURPOSE: In this prospective clinical pilot study, abutments with different topologies (machined versus polished) were compared with respect to the clinical outcome and the microbiological profile. Furthermore, three different sampling methods (retrieval of abutment, collection of peri-abutment exudate using paper-points, and a small peri-abutment soft-tissue biopsy) were evaluated for the identification and quantification of colonising bacteria. METHODS: Twelve patients, seven with machined abutment and five with polished abutment, were included in the analysis...
April 5, 2018: European Archives of Oto-rhino-laryngology
Davide Campoccia, Lucio Montanaro, Stefano Ravaioli, Ilaria Cangini, Francesca Testoni, Livia Visai, Carla Renata Arciola
Complete eradication of bacterial infections is often a challenging task, especially in presence of prosthetic devices. Invasion of non-phagocytic host cells appears to be a critical mechanism of microbial persistence in host tissues. Hidden within host cells, bacteria elude host defences and antibiotic treatments that are intracellularly inactive. The intracellular invasiveness of bacteria is generally measured by conventional gentamicin protection assays. The efficiency of invasion, however, markedly differs across bacterial species and adjustments to the titre of the microbial inocula used in the assays are often needed to enumerate intracellular bacteria...
April 3, 2018: Materials
M Vasil', Z Farkasova, J Elecko, J Illek, F Zigo
In this study we investigated 24 strains of Staphylococcus aureus and 33 strains of Staphylococcus epidermidis isolated from milk of sheep with clinical mastitis, for their ability to form biofilms. Three methods for the determination of a biofilm were used. When evaluating the growth on Congo Red agar (CRA), 79.2% S. aureus strains and 72.7% S. epidermidis strains were positive for biofilm formation. The quantitative method of biofilm detection on a Microtitre Plate (MTP) revealed positive results for 75.0% of S...
December 2017: Polish Journal of Veterinary Sciences
Line Dahlstrøm Christensen, Mai-Britt Skadborg, Agnete H Mortensen, Carsten Mortensen, Jens K Møller, Lars Lemming, Irene Høgsberg, Steffen E Petersen, Niels H Buus
BACKGROUND: The buttonhole cannulation technique for arteriovenous fistulas is widely used, but has been associated with an increased rate of vascular access-related infections. We describe the frequency and type of bacterial colonization of the buttonhole tract over time and associated clinical infections. STUDY DESIGN: A prospective observational cohort study with 9 months of follow-up. SETTING & PARTICIPANTS: 84 in-center hemodialysis patients using the buttonhole cannulation technique at 2 Danish dialysis centers...
March 29, 2018: American Journal of Kidney Diseases: the Official Journal of the National Kidney Foundation
Ya-Ting Bao, Yue Wang, Xiao-Dong Ren, Lin-Fang Huang, Rui Zeng
This paper aimed to investigate the antibacterial activity of flowers and leaves from Paeonia rockii, screen antibacterial compounds and predict targets of antibacterial to explore its multi-component, multi-target antibacterial mechanism. In this study, minimal inhibitory concentration(MIC) of seven strains of Staphylococcus aureus, S. epidermidis, Bacillus subtilis, B. cereus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa were determined by microdilution method. Uniprot databases was used to find the antibacterial targets, and RCSB was used to identify targets associated with antimicrobial activity as docked receptor proteins...
February 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Jinlun Feng, Baomo Liu, Junwen Xu, Qinqin Wang, Lixia Huang, Weijun Ou, Jincui Gu, Jian Wu, Shaoli Li, Chao Zhuo, Yanbin Zhou
Background: Acinetobacter baumannii ( A. baumannii ), as a common opportunistic pathogen, has strong ability to form biofilms, which has led to drug resistance and chronic infections. The combination of N-acetylcysteine (NAC) and tigecycline (TGC) was demonstrated to synergistically inhibit biofilm-associated bacterial infections, including methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. The purpose of this study is to investigate the effect of NAC and TGC on planktonic cells and biofilms of A...
January 2018: Journal of Thoracic Disease
Graziela Rangel Silveira, Karoline Azerêdo Campelo, Gleice Rangel Silveira Lima, Lais Pessanha Carvalho, Solange Silva Samarão, Olney Vieira-da-Motta, Leda Mathias, Carlos Roberto Ribeiro Matos, Ivo José Curcino Vieira, Edesio José Tenório de Melo, Edmilson José Maria
Most cinnamic acids, their esters, amides, aldehydes, and alcohols present several therapeutic actions through anti-inflammatory, antitumor, and inhibitory activity against a great variety of microorganisms. In this work, eight amines derived from cinnamic acid were synthesized and tested against host cells infected with Toxoplasma gondii and the bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, and three strains of Staphylococcus aureus . Compounds 3 and 4 showed the best result against intracellular T...
March 28, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Ze Jing, Kemao Xiu, Xuehong Ren, Yuyu Sun
A series of amine-based cationic polymeric N-halamine precursors, poly(2,2,6,6-tetramethyl-4-piperidyl methacrylate-co-trimethyl-2-methacryloxyethylammonium chloride)(PMPQ), were synthesized by copolymerizing 2,2,6,6-tetramethyl-4-piperidyl methacrylate (TMPM) with trimethyl-2-methacryloxyethylammonium chloride (TMAC) at different molar ratios (TMPM:TMAC = 10:90,30:70,50:50,70:30, and 90:10). After chlorine bleach treatment, the TMPM moieties in the new copolymers were transformed into amine-based N-halamines (Cl-PMPQ)...
March 21, 2018: Colloids and Surfaces. B, Biointerfaces
Bakwena Ashton Hetsa, Ajay Kumar, Collins Njie Ateba
Vagina which is one of the important reservoirs for Staphylococcus and in pregnant women pathogenic strains may infect the child during the birth or by vertical transmission. A total of 68 presumptive Staphylococcus strains isolated from human vagina were found to be gram-positive cocci, and only 32 (47%) isolates were found beta-hemolytic. Matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS) results confirmed 33 isolates belonged to Staphylococcus which consisting of 6 species, i...
March 29, 2018: Folia Microbiologica
Muhammad Ovais, Ali Talha Khalil, Abida Raza, Nazar Ul Islam, Muhammad Ayaz, Muthupandian Saravanan, Muhammad Ali, Irshad Ahmad, Muhammad Shahid, Zabta Khan Shinwari
Phytochemicals offer immense promise for sustainable development and production of nanotechnology-enabled products. In the present study, Olax nana Wall. ex Benth. (family: Olacaceae) aqueous extract was used as an effective stabilizing agent to produce biogenic silver (ON-AgNPs) and gold nanoparticles (ON-AuNPs), which were investigated for biocompatibility and prospective biomedical applications (antibacterial, anticancer, antileishmanial, enzyme inhibition, antinociceptive, and anti-inflammatory activities)...
March 29, 2018: Applied Microbiology and Biotechnology
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