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Ameer Khusro, Chirom Aarti, Abdelfattah Z M Salem, German Buendía-Rodríguez, Raymundo Rene Rivas-Cáceres
The desideratum aim of the present context was to isolate a promising antagonist probiotic bacterium from fermented food item as biocontrol agent against uropathogens. Among diversified isolates evaluated for antagonistic trait, Staphylococcus succinus strain AAS2 was found to be an auspicious candidate against urinary tract infection (UTI) causing bacterial pathogens, being the most active against Staphylococcus aureus with substantial activity of 352.5 ± 5.4 AU/mL. Further, the in vitro probiotic attributes of strain AAS2 was assessed using systematic methodology...
March 14, 2018: Microbial Pathogenesis
Hanwen Tian, Junming Miao, Fumei Zhang, Feng Xiong, Feimei Zhu, Jinyu Li, Xiaoying Wang, Shanzhe Chen, Junli Chen, Ning Huang, Yi Wang
The urinary tract is vulnerable to frequent challenges from environmental microflora. Uropathogenic Escherichia coli (UPEC) makes a major contribution to urinary tract infection (UTI). Previous studies have characterized positive roles of non-histone nuclear protein HMGN2 in lung epithelial innate immune response. In the study presented here, we found HMGN2 expression was up-regulated in UPEC J96-infected urothelium. Surprisingly, over-expression of HMGN2 promoted disruption of BECs 5637 cells' intercellular junctions by down-regulating tight junction (TJs) components' expression and physical structure under J96 infection...
March 17, 2018: Acta Biochimica Polonica
Aixia Xu, Sarah Hertrich, David S Needleman, Shiowshuh Sheen, Christopher Sommers
Uropathogenic Escherichia coli serotype O4:H5 isolates (ATCC 700414, 700415, 700416, and 700417) were recovered from women with first-time urinary tract infections. Here, we report the draft genome sequences for these four E. coli isolates, which are currently being used to validate food safety processing technologies.
March 15, 2018: Genome Announcements
Glenn T Werneburg, David G Thanassi
Gram-negative bacteria assemble a variety of surface structures, including the hair-like organelles known as pili or fimbriae. Pili typically function in adhesion and mediate interactions with various surfaces, with other bacteria, and with other types of cells such as host cells. The chaperone/usher (CU) pathway assembles a widespread class of adhesive and virulence-associated pili. Pilus biogenesis by the CU pathway requires a dedicated periplasmic chaperone and integral outer membrane protein termed the usher, which forms a multifunctional assembly and secretion platform...
March 2018: EcoSal Plus
Catherine S Forster, Elizabeth Jackson, Qing Ma, Michael Bennett, Samir S Shah, Stuart L Goldstein
BACKGROUND: Distinguishing between urinary tract infection (UTI) and colonization (UTC) in patients with neurogenic bladders who require clean intermittent catheterization (CIC) is difficult. Urinary neutrophil gelatinase-associated lipocalin concentrations (uNGAL) are increased in UTIs. Our objective was to determine the predictive accuracy of uNGAL for UTI in CIC-dependent children. METHODS: Cross-sectional study of CIC-dependent patients from August, 2015 to November, 2016...
March 12, 2018: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
Mohd Rizwan, Mohd Akhtar, Abul Kalam Najmi, Kuldeep Singh
AIM: This study was carried out to record the sensitivity/resistance pattern of Escherichia coli and Klebsiella pneumoniae towards antimicrobial drugs prescribed to urinary tract infection (UTI) patients. MATERIALS AND METHODS: A total of 68 bacteriologically proven simple UTI patients were included in the study, based on inclusion and exclusion criteria. Subjects demographics, antibiotics prescribed, sensitivity pattern of antibiotics towards the urinary pathogens were recorded in drug utilization and documentation form...
March 12, 2018: Drug Research
Govindan Rajivgandhi, Ramachandran Vijayan, Muthuchamy Maruthupandy, Baskaralingam Vaseeharan, Natesan Manoharan
Urinary tract infections (UTIs) are a diverse public health complication and are caused by range of pathogens, however most often by Gram negative bacteria which cause significant life threatening risks to different populations. The prevalence rate and antimicrobial resistance among the Gram negative uropathogens alarmed to significantly heighten the economic burden of these infections. In this study, we investigated the role of Pyrrolo[1,2-a] pyrazine-1,4-dione,hexahydro-3-(2-methylpropyl) on endophytic actinomycetes Nocardiopsis sp...
March 7, 2018: Microbial Pathogenesis
Kostis Gyftopoulos
Recurrent urinary tract infections (rUTI) in women is a common cause of morbidity worldwide. In young women recurrent cystitis associated with sexual intercourse is quite common; however not all sexually active women will suffer a post-coital UTI episode. A variety of possible predisposing factors has been described, including behavioral risk patterns, genetic factors and increased virulence of certain subtypes of uropathogens. It has long been regarded that anatomical anomalies are rare in this setting of rUTIs...
April 2018: Medical Hypotheses
Ulises Garza-Ramos, Elsa Tamayo-Legorreta, Doris María Arellano-Quintanilla, Nadia Rodriguez-Medina, Jesús Silva-Sanchez, Juan Catalan-Najera, Marisol Karina Rocha-Martínez, María Asunción Bravo-Díaz, Celia Alpuche-Aranda
A colistin-resistant mcr-1 -carrying Escherichia coli strain, RC2-007, was isolated from a swine farm in Mexico. This extraintestinal and uropathogenic strain of E. coli belongs to serotype O89:H9 and sequence type 744. Assembly and annotation resulted in a 4.9-Mb draft genome that revealed the presence of plasmid-mediated mcr-1 -IS ApI1 genes as part of a prophage.
March 8, 2018: Genome Announcements
Anna Lena Zogg, Katrin Zurfluh, Sarah Schmitt, Magdalena Nüesch-Inderbinen, Roger Stephan
Among 64 uropathogenic Escherichia coli (UPEC) isolated from 13 cats and 51 dogs, 35 were extended-spectrum beta-lactamase (ESBL) producers, and 29 were non-ESBL producers. Forty-six (71.9%) of the isolates were multidrug resistant (MDR). Among the ESBL producers, blaCTX-M-15 (n = 17/48.6% of the blaESBLs ), blaCTX-M-1 (n = 10/28.6%), blaCTX-M-55 (n = 4/11.4%), blaCTX-M-14 (n = 3/8.6%), and blaCTX-M-27 (n = 1/2.9%) were identified. The plasmid-mediated fluoroquinolone resistance genes aac(6')-Ib-cr, qnrB and the azithromycin resistance gene mph(A) were detected in 17 (26...
March 2018: Veterinary Microbiology
Yoshihiko Maeyama, Yui Taniguchi, Wataru Hayashi, Yusuke Ohsaki, Shunsuke Osaka, Shota Koide, Kiyoko Tamai, Yukiko Nagano, Yoshichika Arakawa, Noriyuki Nagano
In recent years, besides the widespread occurrence of extended-spectrum β-lactamase (ESBL)- and/or plasmid-mediated AmpC (pAmpC)-producing Enterobacteriaceae in both healthcare and community settings of humans, the third-generation cephalosporin (3GC)-resistant microbes have also been reported from companion animals worldwide. Here, we characterized ESBL- and/or pAmpC-producing Enterobacteriaceae clinical isolates from companion animals. Among the 487 clinical isolates mainly from urine of dogs and cats between May and September 2016, 104 non-repetitive isolates were resistant to the 3GC, and they consisted of 81 of 381 (21...
March 2018: Veterinary Microbiology
Valerie S Forsyth, Chelsie E Armbruster, Sara N Smith, Ali Pirani, A Cody Springman, Matthew S Walters, Greta R Nielubowicz, Stephanie D Himpsl, Evan S Snitkin, Harry L T Mobley
Uropathogenic Escherichia coli (UPEC) strains cause most uncomplicated urinary tract infections (UTIs). These strains are a subgroup of extraintestinal pathogenic E. coli (ExPEC) strains that infect extraintestinal sites, including urinary tract, meninges, bloodstream, lungs, and surgical sites. Here, we hypothesize that UPEC isolates adapt to and grow more rapidly within the urinary tract than other E. coli isolates and survive in that niche. To date, there has not been a reliable method available to measure their growth rate in vivo Here we used two methods: segregation of nonreplicating plasmid pGTR902, and peak-to-trough ratio (PTR), a sequencing-based method that enumerates bacterial chromosomal replication forks present during cell division...
March 6, 2018: MBio
Caitlin N Spaulding, Roger D Klein, Henry L Schreiber, James W Janetka, Scott J Hultgren
The emergence of drug-resistant pathogens has led to a decline in the efficacy of traditional antimicrobial therapy. The rise in resistance has been driven by widespread use, and in some cases misuse, of antibacterial agents in treating a variety of infections. A growing body of research has begun to elucidate the harmful effects of broad-spectrum antibiotic therapy on the beneficial host microbiota. To combat these threats, increasing effort is being directed toward the development of precision antimicrobial therapeutics that target key virulence determinants of specific pathogens while leaving the remainder of the host microbiota undisturbed...
2018: NPJ Biofilms and Microbiomes
Katy Díaz, Luis Espinoza, Alejandro Madrid, Leonardo Pizarro, Rolando Chamy
Currently, the most effective treatment for recurrent urinary tract infections in women is antibiotics. However, the limitation for this treatment is the duration and dosage of antibiotics and the resistance that bacteria develop after a long period of administration. With the aim of identifying mainly novel natural agents with antibacterial activity, the present study was undertaken to investigate the biological and phytochemical properties of extracts from the leaves Taraxacum officinale. The structural identification of compounds present in hexane (Hex) and ethyl acetate (AcOEt) extracts was performed by mass spectrometry (GC-MS) spectroscopic techniques and nuclear magnetic resonance (NMR) with the major compounds corresponding to different sesquiterpene lactones ( α -santonin, glabellin, arborescin, and estafiatin), monoterpene (9,10-dimethyltricycle [4...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Vasilios Kalas, Michael E Hibbing, Amarendar Reddy Maddirala, Ryan Chugani, Jerome S Pinkner, Laurel K Mydock-McGrane, Matt S Conover, James W Janetka, Scott J Hultgren
Treatment of bacterial infections is becoming a serious clinical challenge due to the global dissemination of multidrug antibiotic resistance, necessitating the search for alternative treatments to disarm the virulence mechanisms underlying these infections. Uropathogenic Escherichia coli (UPEC) employs multiple chaperone-usher pathway pili tipped with adhesins with diverse receptor specificities to colonize various host tissues and habitats. For example, UPEC F9 pili specifically bind galactose or N -acetylgalactosamine epitopes on the kidney and inflamed bladder...
March 5, 2018: Proceedings of the National Academy of Sciences of the United States of America
Taylor I Herring, Tiffany N Harris, Chiranjit Chowdhury, Sujit Mohanty, Thomas A Bobik
Bacterial choline degradation in the human gut has been associated with cancer and heart disease. In addition, recent studies found that a bacterial microcompartment is involved in choline utilization by Proteus and Desulfovibrio However, many aspects of this process have not been fully defined. Here, we investigate choline degradation by the uropathogen Escherichia coli 536. Growth studies indicated E. coli 536 degrades choline primarily by fermentation. Electron microscopy indicated that a bacterial microcompartment was used for this process...
March 5, 2018: Journal of Bacteriology
S Sarshar, J Sendker, X Qin, F M Goycoolea, M R Asadi Karam, M Habibi, S Bouzari, U Dobrindt, A Hensel
Fruits from Apium graveolens (Celery) are used traditionally in Persian and European medicine for the treatment of uncomplicated urinary tract infections. No data are available on A. graveolens extract on the interplay between uropathogenic E. coli and the eukaryotic host cells and on quorum sensing of the bacteria. The present study aimed to characterize an antiadhesive and anti quorum sensing effect of a characterized A. graveolens extract by specific in vitro assays and to correlate these effects with in vivo data obtained by an animal infection model...
February 26, 2018: Fitoterapia
Christoph Stork, Beáta Kovács, Barnabás Rózsai, Johannes Putze, Matthias Kiel, Ágnes Dorn, Judit Kovács, Szilvia Melegh, Andreas Leimbach, Tamás Kovács, György Schneider, Monika Kerényi, Levente Emödy, Ulrich Dobrindt
Asymptomatic bacterial colonization of the urinary bladder (asymptomatic bacteriuria, ABU) can prevent bladder colonization by uropathogens and thus symptomatic urinary tract infection (UTI). Deliberate bladder colonization with Escherichia coli ABU isolate 83972 has been shown to outcompete uropathogens and prevent symptomatic UTI by bacterial interference. Many ABU isolates evolved from uropathogenic ancestors and, although attenuated, may still be able to express virulence-associated factors. Our aim was to screen for efficient and safe candidate strains that could be used as alternatives to E...
2018: Frontiers in Microbiology
Yongning Lu, Amir Rafiq, Zhengguo Zhang, Ferial Aslani, Monika Fijak, Tao Lei, Ming Wang, Sanjeev Kumar, Jörg Klug, Martin Bergmann, Trinad Chakraborty, Andreas Meinhardt, Sudhanshu Bhushan
Uropathogenic Escherichia coli (UPEC) is the most common cause of urinary tract infections. In this study, UPEC strains harboring hemolysin A (HlyA) did not induce programmed cell death pathways by the activation of caspases. Instead, the UPEC pore-forming toxin HlyA triggered an increase in mitochondrial Ca2+ levels and manipulated mitochondrial dynamics by causing fragmentation of the mitochondrial network. Alterations in mitochondrial dynamics resulted in severe impairment of mitochondrial functions by loss of membrane potential, increase in ROS production, and ATP depletion...
February 28, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Birhanu Ayelign, Betelehem Abebe, Adugna Shibeshi, Sosina Meshesha, Tewodros Shibabaw, Zelalem Addis, Aschalew Gelaw, Mulat Dagnew
Objective: Urinary tract infection is a common pediatric problem with the potential to produce long-term morbidity. Therefore, appropriate diagnosis and prompt treatment is required. However, studies about magnitude of uropathogenicity and antimicrobial resistance pattern of pediatric urinary tract infection (UTI) are lacking in resource limited countries including Ethiopia. This study was aimed to determine bacterial isolates, antimicrobial susceptibility pattern among pediatric patients with UTI...
January 2018: Turkish Journal of Urology
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