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https://www.readbyqxmd.com/read/29150650/transcriptome-sequencing-and-differential-gene-expression-analysis-of-the-schistosome-transmitting-snail-oncomelania-hupensis-inhabiting-hilly-and-marshland-regions
#1
Jin-Song Zhao, An-Yun Wang, Hua-Bin Zhao, Yan-Hong Chen
The freshwater snail Oncomelania hupensis is the unique intermediate host of the blood fluke Schistosoma japonicum, which is the major cause of schistosomiasis. The snail inhabits two contrasting environments: the hilly and marshland regions. The hilly snails are smaller in size and have the typical smooth shell, whereas the marshland snails are larger and possess the ribbed shell. To reveal the differences in gene expression between the hilly and marshland snails, a total of six snails, three per environment, were individually examined by RNA sequencing technology...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29143720/epidemiological-investigation-of-a-severe-rumen-fluke-outbreak-on-an-irish-dairy-farm
#2
James O'Shaughnessy, Andres Garcia-Campos, Conor G McAloon, Seamus Fagan, Theo de Waal, Maire McElroy, Micheal Casey, Barbara Good, Grace Mulcahy, John Fagan, Denise Murphy, Annetta Zintl
Although the rumen fluke, Calicophoron daubneyi is now very common and widespread throughout Western Europe, reports of clinical cases are still rare. This study explores the epidemiological background to a severe rumen fluke outbreak in 6-month-old heifers on a dairy farm in Ireland. Sequence analysis of the cytochrome oxidase subunit 1 (Cox1) gene of the rumen fluke metacercariae on pasture failed to identify predominant, possibly pathogenic subtypes. However, estimates of metacercarial load indicated that the animals were exposed to a daily dose of about 5334 C...
November 16, 2017: Parasitology
https://www.readbyqxmd.com/read/29132905/purification-and-biochemical-characterization-of-a-22-kda-stable-cysteine-like-protease-from-the-excretory-secretory-product-of-the-liver-fluke-fasciola-hepatica-by-using-conventional-techniques
#3
Ahmed Hemici, Roumaila Sabrina Benerbaiha, Dalila Bendjeddou
This study describes the purification and characterization of a stable protease activity isolated from Fasciola hepatica adult worms maintained in vitro by employing acetone precipitation (40-60%) followed by a gel filtration through Sephadex G-100 and DEAE- cellulose ion exchange column. Through this three-step purification, the enzyme was purified 11-fold with a specific activity of 1893.9U/mg and 31.5% recovery. After the final ultrafiltration step, the purification fold was increased up to 13.1 and the overall activity yield reached a rate of 18...
October 28, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29121986/farm-level-risk-factors-for-fasciola-hepatica-infection-in-danish-dairy-cattle-as-evaluated-by-two-diagnostic-methods
#4
Nao Takeuchi-Storm, Matthew Denwood, Tina Vicky Alstrup Hansen, Tariq Halasa, Erik Rattenborg, Jaap Boes, Heidi Larsen Enemark, Stig Milan Thamsborg
BACKGROUND: The prevalence of bovine fasciolosis in Denmark is increasing but appropriate guidelines for control are currently lacking. In order to help develop a control strategy for liver fluke, a risk factor study of farm management factors was conducted and the utility of bulk tank milk (BTM ELISA) as a tool for diagnosis in Danish dairy cattle farms was assessed. METHODS: This case-control study aimed to identify farm-level risk factors for fasciolosis in Danish dairy farms (> 50 animals slaughtered in 2013) using two diagnostic methods: recordings of liver condemnation at slaughter, and farm-level Fasciola hepatica antibody levels in BTM...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29118312/dynamics-of-liver-enzymes-in-rabbits-experimentally-infected-with-fasciola-sp-intermediate-form-from-japan
#5
Wipaporn Jarujareet, Kensuke Taira, Hong Kean Ooi
Dynamics of serum liver enzymes in rabbits experimentally infected with metacercariae of Fasciola sp. (intermediate form between Fasciola hepatica and F. gigantica) were monitored. Gradual increase of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were observed from 3 weeks post-inoculation (WPI) and peaked at 6 WPI, which corresponded well to the period of migration and development of juvenile fluke in the liver parenchyma and the time when the young adult flukes migrated to the bile duct...
November 9, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29114486/morphological-and-histological-analysis-of-cotylophoron-cotylophorum-treated-with-acacia-concinna
#6
P Priya, L Veerakumari
Objective: Paramphistomosis (stomach fluke disease) is a parasitic infection caused by digenetic trematodes and is considered to be one of the most important parasitic diseases affecting livestock worldwide. This disease is widely prevalent in India, and the highest incidence is reported during monsoon and post-monsoon months. In the present study, in vitro effect of aqueous extract of pods of Acacia concinna (AcP E) on the morphology and the histology of the digenetic trematode Cotylophoron cotylophorum have been investigated...
July 2017: Tropical Parasitology
https://www.readbyqxmd.com/read/29110582/establishment-of-cholangiocarcinoma-cell-lines-from-patients-in-the-endemic-area-of-liver-fluke-infection-in-thailand
#7
Sunitta Saensa-Ard, Saman Leuangwattanawanit, Laddawan Senggunprai, Nisana Namwat, Sarinya Kongpetch, Yaovalux Chamgramol, Watcharin Loilome, Walaiporn Khansaard, Apinya Jusakul, Auemduan Prawan, Chawalit Pairojkul, Narong Khantikeo, Puangrat Yongvanit, Veerapol Kukongviriyapan
Cholangiocarcinoma is a rare type of cancer which is an increasingly discernible health threat. The disease is usually very difficult in diagnosis and various treatment modalities are typically not effective. Cholangiocarcinoma is a complex and very heterogeneous malignancy characterized by tumor location, different risk factors, molecular profiling, and prognosis. Cancer cell lines represent an important tool for investigation in various aspects of tumor biology and molecular therapeutics. We established two cell lines, KKU-452 and KKU-023, which were derived from patients residing in the endemic area of liver fluke infection in Thailand...
November 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/29103276/intestinal-parasite-infections-among-inhabitants-in-yanbian-prefecture-jilin-province-china
#8
Myoung-Ro Lee, Hee-Eun Shin, Byung-Suk Chung, Sang-Eun Lee, Jung-Won Ju, Liji Xu, Chen Long Nan, Mi-Yeoun Park, Shin-Hyeong Cho
To investigate the prevalence of intestinal parasite infections in Yanbian Prefecture, Jilin Province, China, epidemiological surveys were conducted on a collaboration basis between the Korean Centers for Disease Control and Prevention and the Yanbian Center for Disease Control and Prevention. A total of 8,396 (males 3,737 and females 4,659) stool samples were collected from 8 localities and examined with the formalin-ether sedimentation technique, and additionally examined with the cellotape anal swab to detect Enterobius vermicularis eggs...
October 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29103269/echinostoma-macrorchis-digenea-echinostomatidae-metacercariae-in-cipangopaludina-chinensis-malleata-snails-and-adults-from-experimental-rats-in-korea
#9
Woon-Mok Sohn, Byoung-Kuk Na
A survey was performed to know the infection status of echinostome metacercariae in Cipangopaludina chinensis malleata snails from Korea. Total 75 snails collected in 5 localities, i.e., Imsil-gun, Jeollabuk-do, Hwasun-gun and Shinan-gun (Aphae and Jido), Jeollanam-do, and Jinju-si, Gyeongsangnam-do, were examined for metacercariae by the artificial digestion method. Infection rates of metacercariae were 80.0%, 66.7%, 100%, 60.0%, and 73.3%, and their densities were 39, 32, 183, 19, and 30 per snail infected, respectively...
October 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29100926/blood-flukes-cardicola-parvus-and-c-laruei-trematoda-aporocotylidae-life-cycles-and-cryptic-infection-in-spotted-seatrout-cynoscion-nebulosus-teleost-sciaenidae
#10
Sasha V Siegel, Andrea Rivero, Jenna Oberstaller, Beatrice L Colon, Isaure de Buron, Dennis E Kyle
Aporocotylidae comprises a diverse family of fish blood flukes, with adults found in blood or body cavity of marine, brackish, or freshwater fish. Aporocotylids are unique among the Digenea with many developing in polychaetes. The life cycle has been elucidated for only a few species that develop in polychaetes from marine/brackish environments and none for western Atlantic aporocotylids. The basis for this study was observations of blood fluke larvae in terebellids from South Carolina, USA in 1982 prior to possible usage of molecular tools to specifically identify parasite larvae...
October 31, 2017: Parasitology International
https://www.readbyqxmd.com/read/29095828/molecular-discrimination-of-opisthorchis-like-eggs-from-residents-in-a-rural-community-of-central-thailand
#11
Saiwasan Buathong, Saovanee Leelayoova, Mathirut Mungthin, Toon Ruang-Areerate, Tawee Naaglor, Picha Suwannahitatorn, Phunlerd Piyaraj, Paanjit Taamasri, Peerapan Tan-Ariya
BACKGROUND: Opisthorchis viverrini infection is a major public health problem in northern and northeastern Thailand. The chronic infection of O. viverrini is related to cholangiocarcinoma which causes high mortality in endemic areas. Therefore, the diagnosis, treatment, control and prevention of O. viverrini infection are necessary. The morphology of the egg is very similar to that of other species of human liver flukes (Opisthorchis felineus and Clonorchis sinensis) as well as that of small intestinal flukes in the family Heterophyidae...
November 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29080772/the-american-mink-neovison-vison-is-a-competent-host-for-native-european-parasites
#12
F J Martínez-Rondán, M R Ruiz de Ybáñez, P Tizzani, A M López-Beceiro, L E Fidalgo, C Martínez-Carrasco
The American mink (Neovison vison) is a mustelid native to North America that was introduced in Europe and the former USSR for fur farming. Throughout the last century, accidental or deliberate escapes of mink from farms caused the establishment of stable feral populations. In fact, the American mink is considered an invasive alien species in 28 European countries. The present study evaluates the gastrointestinal and cardiopulmonary helminth fauna of the American mink in Galicia (NW Spain) to understand its role as a potential reservoir for parasites affecting other autochthonous mustelids...
November 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/29080760/diagnostic-accuracy-of-the-light-microscope-method-to-detect-the-eggs-of-cardicola-spp-in-the-gill-filaments-of-the-bluefin-tuna
#13
José F Palacios-Abella, Javier Rodríguez-Llanos, María Víllora-Montero, Salvatore Mele, Juan A Raga, Francisco E Montero
Trematode blood flukes of the genus Cardicola are potentially lethal in bluefin tuna cultures. The present study proposed a new method to detect aporocotylid eggs in tuna gills. Aporocotylid eggs were detected by analysing a pair of gill filaments of five transversal areas of the eight holobranches of one hundred Atlantic bluefin tuna and observed with glycerol and a stereomicroscope with an oblique brightfield. Data were gathered according to holobranches, transversal areas and their combination. Eggs were uniformly distributed among the holobranches, but they had the highest prevalence in the second and fifth transversal areas, which is controversial with respect to previous studies of egg distribution...
November 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/29075480/control-of-nematodirus-spp-infection-by-sheep-flock-owners-in-northern-ireland
#14
Connor McMahon, Hillary W J Edgar, Jason P Barley, Robert E B Hanna, Gerard P Brennan, Ian Fairweather
BACKGROUND: To address a lack of information on the control of ovine helminth parasites in Northern Ireland (NI), a number of research projects have been undertaken, dealing with gastrointestinal nematodes, tapeworms and liver fluke. This investigation concerns Nematodirus and concentrates on three aspects of disease: farm management strategies for its control, derived from the results of a Questionnaire; the efficacy of treatment used by farmers, as determined by a coprological survey; and the hatching requirements of Nematodirus eggs, that is, whether prolonged chilling is a pre-requisite for hatching...
2017: Irish Veterinary Journal
https://www.readbyqxmd.com/read/29074138/schistosoma-mansoni-in%C3%A2-vivo-evaluation-of-phyllanthus-amarus-hexanic-and-ethanolic-extracts
#15
Claudineide Nascimento Fernandes de Oliveira, Tarsila Ferraz Frezza, Vera Lúcia Garcia, Glyn Mara Figueira, Tiago Manuel Fernandes Mendes, Silmara Marques Allegretti
Human schistosomiasis is an important neglected tropical disease caused by blood flukes of the genus Schistosoma and is responsible for more than 280,000 deaths annually. Treatment for this disease relies currently on a single drug, praziquantel (PZQ). Concerns regarding PZQ resistance and insensitivity of juvenile schistosomes have increased the interest in resorting to medicinal plants for alternative drug therapies. This study aimed to perform an in vivo schistosomicidal activity evaluation of crude hexanic (HE) and ethanolic (EE) extracts obtained from Phyllanthus amarus in mice infected with Schistosoma mansoni (BH strain)...
October 23, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/29072436/a-large-scale-study-of-the-epidemiology-and-risk-factors-for-the-carcinogenic-liver-fluke-opisthorchis-viverrini-in-udon-thani-province-thailand
#16
Suksanti Prakobwong, Apiporn Suwannatrai, Achara Sancomerang, Suwit Chaipibool, Ngampis Siriwechtumrong
Opisthorchis viverrini infection and cholangiocarcinoma are serious problems in South East Asia. This study aimed to find the prevalence of opisthorchiasis in various hosts in Udon Thani Province. Total fecal samples were collected from 14,766 participants. The epidemiological data collected and analysed included prevalence and intensity of infection. Odds ratios (OR) were calculated to determine the associations between cross sectional data and to predict possible risk factors. The prevalence of O. viverrini infection in Udon Thani Province averaged 15...
October 26, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29052139/parasite-associated-cancers-blood-flukes-liver-flukes
#17
Meng Feng, Xunjia Cheng
Parasitic infection remains as a persistent public health problem and can be carcinogenic. Three helminth parasites, namely, Clonorchis sinensis (liver fluke) and Opisthorchis viverrini as well as Schistosoma haematobium (blood fluke), are classified as Group 1 carcinogens by the World Health Organization's International Agency for Research on Cancer (IARC Infection with liver flukes (Opisthorchis viverrini, Opisthorchis felineus and Clonorchis sinensis), World Health Organization, International Agency for Research on Cancer, 2011)...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29045404/transcriptomic-responses-of-biomphalaria-pfeifferi-to-schistosoma-mansoni-investigation-of-a-neglected-african-snail-that-supports-more-s-mansoni-transmission-than-any-other-snail-species
#18
Sarah K Buddenborg, Lijing Bu, Si-Ming Zhang, Faye D Schilkey, Gerald M Mkoji, Eric S Loker
BACKGROUND: Biomphalaria pfeifferi is highly compatible with the widespread human-infecting blood fluke Schistosoma mansoni and transmits more cases of this parasite to people than any other snail species. For these reasons, B. pfeifferi is the world's most important vector snail for S. mansoni, yet we know relatively little at the molecular level regarding the interactions between B. pfeifferi and S. mansoni from early-stage sporocyst transformation to the development of cercariae. METHODOLOGY/PRINCIPAL FINDINGS: We sought to capture a portrait of the response of B...
October 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29035851/mitochondrial-genotyping-of-fascioloides-magna-from-bavaria-germany
#19
Eva Bazsalovicsová, Ludmila Juhásová, Ivica Králová-Hromadová, Steffen Rehbein
In last few years, a great effort has been made to understand genetic interrelationships of European and North American populations of giant liver fluke Fascioloides magna (Trematoda, Fasciolidae). In Europe, spatial distribution of this parasite is evidently dynamic and ongoing process since new F. magna populations have constantly been emerging. Most recently, occurrence of F. magna in red deer (Cervus elaphus), sika deer (Cervus nippon), fallow deer (Dama dama), roe deer (Capreolus capreolus), and wild boar (Sus scrofa) was reported from north-eastern Bavaria in Germany...
December 20, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/29035247/importance-of-land-snails-in-dicrocoeliosis-epidemiology
#20
Gözde Gürelli
Dicrocoeliosis is a helminthosis caused by the small liver fluke Dicrocoelium spp. (Trematoda, Dicrocoeliidae) parasitizing in the bile ducts and gall bladder of ruminants as well as many other animal species including humans. In the biological life cycle of Dicrocoelium, land snails are first intermediate hosts and ants are second intermediate hosts. Sporocysts and cercaria, which are larval stages, live in the hepatopancreas of land snails and metacercaria, which is also the larval stage, lives in the abdomen and brain of ants...
September 2017: Türkiye Parazitolojii Dergisi
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