Sile F Yang, Christopher B Nelson, Jadon K Wells, Madushan Fernando, Robert Lu, Joshua A M Allen, Lisa Malloy, Noa Lamm, Vincent J Murphy, Joel P Mackay, Andrew J Deans, Anthony J Cesare, Alexander P Sobinoff, Hilda A Pickett
The ATR-CHK1 DNA damage response pathway becomes activated by the exposure of RPA-coated single-stranded DNA (ssDNA) that forms as an intermediate during DNA damage and repair, and as a part of the replication stress response. Here, we identify ZNF827 as a component of the ATR-CHK1 kinase pathway. We demonstrate that ZNF827 is a ssDNA binding protein that associates with RPA through concurrent binding to ssDNA intermediates. These interactions are dependent on two clusters of C2H2 zinc finger motifs within ZNF827...
March 12, 2024: Nature Communications