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Aldolase b

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https://www.readbyqxmd.com/read/29669261/chrebp-deficiency-leads-to-diarrhea-predominant-irritable-bowel-syndrome
#1
Ah-Reum Oh, Seonyong Sohn, Junghoon Lee, Jong-Min Park, Ki Taek Nam, Ki-Baik Hahm, Young-Bum Kim, Ho-Jae Lee, Ji-Young Cha
OBJECTIVE: Fructose malabsorption is a common digestive disorder in which absorption of fructose in the small intestine is impaired. An abnormality of the main intestinal fructose transporter proteins has been proposed as a cause for fructose malabsorption. However the underlying molecular mechanism for this remains unclear. In this study, we investigated whether carbohydrate response element-binding protein (ChREBP) plays a role in intestinal fructose absorption through the regulation of genes involved in fructose transport and metabolism and ion transport...
April 15, 2018: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/29619215/proteomic-analysis-of-adipose-tissue-during-the-last-weeks-of-gestation-in-pure-and-crossbred-large-white-or-meishan-fetuses-gestated-by-sows-of-either-breed
#2
F Gondret, B Guével, M C Père, H Quesnel, Y Billon, E Com, L Canario, I Louveau, L Liaubet
Background: The degree of adipose tissue development at birth may influence neonatal survival and subsequent health outcomes. Despite their lower birth weights, piglets from Meishan sows (a fat breed with excellent maternal ability) have a higher survival rate than piglets from Large White sows (a lean breed). To identify the main pathways involved in subcutaneous adipose tissue maturation during the last month of gestation, we compared the proteome and the expression levels of some genes at d 90 and d 110 of gestation in purebred and crossbred Large White or Meishan fetuses gestated by sows of either breed...
2018: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29588660/lignolytic-consortium-omics-analyses-reveal-novel-genomes-and-pathways-involved-in-lignin-modification-and-valorization
#3
Eduardo C Moraes, Thabata M Alvarez, Gabriela F Persinoti, Geizecler Tomazetto, Livia B Brenelli, Douglas A A Paixão, Gabriela C Ematsu, Juliana A Aricetti, Camila Caldana, Neil Dixon, Timothy D H Bugg, Fabio M Squina
Background: Lignin is a heterogeneous polymer representing a renewable source of aromatic and phenolic bio-derived products for the chemical industry. However, the inherent structural complexity and recalcitrance of lignin makes its conversion into valuable chemicals a challenge. Natural microbial communities produce biocatalysts derived from a large number of microorganisms, including those considered unculturable, which operate synergistically to perform a variety of bioconversion processes...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29533924/ketohexokinase-c-blockade-ameliorates-fructose-induced-metabolic-dysfunction-in-fructose-sensitive-mice
#4
Miguel A Lanaspa, Ana Andres-Hernando, David J Orlicky, Christina Cicerchi, Cholsoon Jang, Nanxing Li, Tamara Milagres, Masanari Kuwabara, Michael F Wempe, Joshua D Rabinowitz, Richard J Johnson, Dean R Tolan
Increasing evidence suggests a role for excessive intake of fructose in the Western diet as a contributor to the current epidemics of metabolic syndrome and obesity. Hereditary fructose intolerance (HFI) is a difficult and potentially lethal orphan disease associated with impaired fructose metabolism. In HFI, the deficiency of a particular aldolase, aldolase B, results in the accumulation of intracellular phosphorylated fructose thus leading to phosphate sequestration and depletion, increased ATP turnover and a plethora of conditions leading to clinical manifestations including fatty liver, hyperuricemia, Fanconi syndrome and severe hypoglycemia...
March 13, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29518347/dietary-genistein-supplementation-in-laying-broiler-breeder-hens-alters-the-development-and-metabolism-of-offspring-embryos-as-revealed-by-hepatic-transcriptome-analysis
#5
Zengpeng Lv, Hao Fan, Beibei Zhang, Chao Ning, Kun Xing, Yuming Guo
Genistein (GEN) is a type of isoflavone mainly derived from soy products. In this experiment, we added 40 and 400 mg/kg GEN to the diet of laying broiler breeder hens to clarify the maternal effects of GEN on the development and metabolism of chick embryos. GEN treatment at 40 mg/kg increased embryonic length, weight, and liver index, as well as the width of the proliferative zone in the tibial growth plate of chick embryos. Gene ontology (GO) cluster analysis of the hepatic transcriptome showed that GEN treatment promoted embryonic development and cell proliferation...
March 8, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29510902/acute-liver-failure-in-neonates-with-undiagnosed-hereditary-fructose-intolerance-due-to-exposure-from-widely-available-infant-formulas
#6
Hong Li, Heather M Byers, Alicia Diaz-Kuan, Miriam B Vos, Patricia L Hall, Silvia Tortorelli, Rani Singh, Matthew B Wallenstein, Meredith Allain, David P Dimmock, Ryan M Farrell, Shawn McCandless, Michael J Gambello
Hereditary fructose intolerance (HFI) is an autosomal recessive disorder caused by aldolase B (ADOLB) deficiency resulting in an inability to metabolize fructose. The toxic accumulation of intermediate fructose-1-phosphate causes multiple metabolic disturbances, including postprandial hypoglycemia, lactic acidosis, electrolyte disturbance, and liver/kidney dysfunction. The clinical presentation varies depending on the age of exposure and the load of fructose. Some common infant formulas contain fructose in various forms, such as sucrose, a disaccharide of fructose and glucose...
February 27, 2018: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/29466423/proteomic-analysis-of-the-human-retina-reveals-region-specific-susceptibilities-to-metabolic-and-oxidative-stress-related-diseases
#7
Gabriel Velez, Daniel A Machlab, Peter H Tang, Yang Sun, Stephen H Tsang, Alexander G Bassuk, Vinit B Mahajan
Differences in regional protein expression within the human retina may explain molecular predisposition of specific regions to ophthalmic diseases like age-related macular degeneration, cystoid macular edema, retinitis pigmentosa, and diabetic retinopathy. To quantify protein levels in the human retina and identify patterns of differentially-expressed proteins, we collected foveomacular, juxta-macular, and peripheral retina punch biopsies from healthy donor eyes and analyzed protein content by liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
2018: PloS One
https://www.readbyqxmd.com/read/29392388/engineering-escherichia-coli-for-glycolic-acid-production-from-d-xylose-through-the-dahms-pathway-and-glyoxylate-bypass
#8
Rhudith B Cabulong, Won-Keun Lee, Angelo B Bañares, Kristine Rose M Ramos, Grace M Nisola, Kris Niño G Valdehuesa, Wook-Jin Chung
Glycolic acid (GA) is an ⍺-hydroxy acid used in cosmetics, packaging, and medical industries due to its excellent properties, especially in its polymeric form. In this study, Escherichia coli was engineered to produce GA from D-xylose by linking the Dahms pathway, the glyoxylate bypass, and the partial reverse glyoxylate pathway (RGP). Initially, a GA-producing strain was constructed by disrupting the xylAB and glcD genes in the E. coli genome and overexpressing the xdh(Cc) from Caulobacter crescentus. This strain was further improved through modular optimization of the Dahms pathway and the glyoxylate bypass...
February 1, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29374035/functional-characterization-of-three-specific-acyl-coenzyme-a-synthetases-involved-in-anaerobic-cholesterol-degradation-in-sterolibacterium-denitrificans-chol1s
#9
Markus Warnke, Tobias Jung, Christian Jacoby, Michael Agne, Franziska Maria Feller, Bodo Philipp, Wolfgang Seiche, Bernhard Breit, Matthias Boll
The denitrifying betaproteobacterium Sterolibacterium denitrificans Chol1S catabolizes steroids such as cholesterol via an oxygen-independent pathway. It involves enzyme reaction sequences described for aerobic cholesterol and bile acid degradation as well as enzymes uniquely found in anaerobic steroid-degrading bacteria. Recent studies provided evidence that in S. denitrificans , the cholest-4-en-3-one intermediate is oxygen-independently oxidized to Δ4 -dafachronic acid (C26 -oic acid), which is subsequently activated by a substrate-specific acyl-coenzyme A (acyl-CoA) synthetase (ACS)...
April 1, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29349857/a-diazido-mannose-analogue-as-a-chemoenzymatic-synthon-for-synthesizing-di-n-acetyllegionaminic-acid-containing-glycosides
#10
Abhishek Santra, An Xiao, Hai Yu, Wanqing Li, Yanhong Li, Linh Ngo, John B McArthur, Xi Chen
A chemoenzymatic synthon was designed to expand the scope of the chemoenzymatic synthesis of carbohydrates. The synthon was enzymatically converted into carbohydrate analogues, which were readily derivatized chemically to produce the desired targets. The strategy is demonstrated for the synthesis of glycosides containing 7,9-di-N-acetyllegionaminic acid (Leg5,7Ac2 ), a bacterial nonulosonic acid (NulO) analogue of sialic acid. A versatile library of α2-3/6-linked Leg5,7Ac2 -glycosides was built by using chemically synthesized 2,4-diazido-2,4,6-trideoxymannose as a chemoenzymatic synthon for highly efficient one-pot multienzyme (OPME) sialylation followed by downstream chemical conversion of the azido groups into acetamido groups...
March 5, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29250698/genetic-disorder-in-carbohydrates-metabolism-hereditary-fructose-intolerance-associated-with-celiac-disease
#11
Daniela Păcurar, Gabriela Leşanu, Irina Dijmărescu, Iulia Florentina Ţincu, Mihaela Gherghiceanu, Dumitru Orăşeanu
Celiac disease (CD) has been associated with several genetic and immune disorders, but association between CD and hereditary fructose intolerance (HFI) is extremely rare. HFI is an autosomal recessive disease caused by catalytic deficiency of aldolase B (fructose-1,6-bisphosphate aldolase). We report the case of a 5-year-old boy suffering from CD, admitted with an initial diagnosis of Reye's-like syndrome. He presented with episodic unconsciousness, seizures, hypoglycemia, hepatomegaly and abnormal liver function...
2017: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/29247687/genotypic-variations-between-wild-type-and-small-colony-variant-of-staphylococcus-aureus-in-prosthetic-valve-infectious-endocarditis-a-comparative-genomic-and-transcriptomic-analysis
#12
Hongbin Chen, Qi Wang, Yuyao Yin, Shuguang Li, Deng-Ke Niu, Hui Wang
Staphylococcus aureus small colony variants (SCVs) can cause persistent infections. However, the genomes and transcriptomes of S. aureus SCVs remain poorly understood. A pair of isogenic wild-type and SCV methicillin-resistant S. aureus (MRSA) strains (IE1 and IE2, respectively) were isolated from a patient with prosthetic valve infectious endocarditis. The SCV strain IE2 grew more slowly than the wild-type strain, and serum killing and mouse lethality assays revealed that the virulence of SCV strain IE2 was decreased...
December 13, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29241575/dietary-supplementation-with-myo-inositol-reduces-hepatic-triglyceride-accumulation-and-expression-of-both-fructolytic-and-lipogenic-genes-in-rats-fed-a-high-fructose-diet
#13
Masaya Shimada, Masato Hibino, Anna Takeshita
Excessive fructose ingestion drastically enhances hepatic lipid accumulation. The most prominent form of inositol-myo-inositol (MI)-remarkably reduces high sucrose-induced hepatic triglyceride (TG) accumulation. Because MI is a major and strong lipotrope, we hypothesized in this study that MI improves fatty liver more induced by excessive ingestion of fructose than sucrose. Rats were fed a high-glucose diet (HGD), a high-fructose diet (HFD), or an HFD supplemented with 0.2% MI for 12 days. Hepatic levels of TG and mRNAs for fructolysis (ketohexokinase and aldolase B), lipogenesis (pyruvate kinase, liver, and RBC; glucose-6-phosphate dehydrogenase; acetyl-CoA carboxylase alpha; fatty acid synthase; and stearoyl-CoA desaturase 1), and a key transcription factor for lipogenesis-carbohydrate-responsive element-binding protein-were significantly increased in the HFD group compared with the HGD group, and the increase was markedly decreased by MI supplementation...
November 2017: Nutrition Research
https://www.readbyqxmd.com/read/29182758/microrna-122-targets-genes-related-to-goose-fatty-liver
#14
Jun Zhang, Qian Wang, Xing Zhao, Laidi Wang, Xingguo Wang, Jian Wang, Biao Dong, Daoqing Gong
MicroRNA-122 (miR-122), a completely conserved, liver-specific miRNA in vertebrates, is essential for the maintenance of liver homeostasis. This 22-nucleotide-length RNA regulates diverse functions such as cholesterol, glucose, and lipid metabolism as well as iron homeostasis and infection of hepatitis C virus (HCV). Landes goose, which has a good, fatty liver, has important significance for us in studying miR-122 function in goose fatty liver. In the current study, we identified miR-122 in goose liver and its expression pattern and target genes...
February 1, 2018: Poultry Science
https://www.readbyqxmd.com/read/29055862/juvenile-exposure-to-bisphenol-a-promotes-ovarian-differentiation-but-suppresses-its-growth-potential-involvement-of-pituitary-follicle-stimulating-hormone
#15
Weiting Chen, Shuk-Wa Lau, Yuqin Fan, Rudolf S S Wu, Wei Ge
Bisphenol A (BPA), a plastic monomer and plasticizer, is commonly used in plastics industry, and it has been well documented to be an estrogenic endocrine disrupter. In the present study, we investigated the effect of early (juvenile) exposure to BPA on the hypothalamic-pituitary-gonad (HPG) axis in the zebrafish. Estradiol (E2) and testosterone (T) were also included as positive and negative controls respectively. Juvenile zebrafish were exposed to BPA (1 and 10μM), E2 (10nM) and T (10nM) from 20 to 40 dpf (days post-fertilization), the period of sex/gonadal differentiation, followed by histological and expression analyses at 40 dpf...
December 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28973717/tissue-distribution-and-early-developmental-expression-patterns-of-aldolase-a-b-and-c-in-grass-carp-ctenopharyngodon-idellus
#16
J J Fan, J J Bai, D M Ma, L Y Yu, P Jiang
Aldolase is a key enzyme involved in glycolysis, gluconeogenesis, and the pentose phosphate pathway. To establish the expression patterns of all three aldolase isozyme genes in different tissues and during early embryogenesis in lower vertebrates, as well as to explore the functional differences between these three isozymes, the grass carp was selected as a model owing to its relatively high glucose-metabolizing capability. Based on the cDNA sequences of the aldolase A, B, and C genes, the expression patterns of these three isozymes were analyzed in different tissues and during early embryogenesis using quantitative real-time polymerase chain reaction (qRT-PCR)...
September 27, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28955482/decreased-cd3-cd16-cd56-natural-killer-cell-counts-in-children-with-orbital-myositis-a-clue-to-disease-activity
#17
Melissa R Briones, Gabrielle A Morgan, Maria C Amoruso, Bahram Rahmani, Maura E Ryan, Lauren M Pachman
The study aimed to document the utility of the absolute number of natural killer cells as a biomarker in paediatric orbital myositis (OM). Extracted data from four children with OM included demographics, laboratory values, imaging and treatment response. Stored sera (-80°C) were tested for IgG4 levels in three cases and antibody to Coxsackie B in two cases. Their first symptom was at 14.4±1.2 years (mean±SD). At diagnosis three had creatine phosphokinase (CPK) of 97.3±44.2, aldolase of 8.5±2.8 (n=2), alanine aminotransferase (ALT) of 13±2...
2017: RMD Open
https://www.readbyqxmd.com/read/28940833/a-patchwork-pathway-for-oxygenase-independent-degradation-of-side-chain-containing-steroids
#18
Markus Warnke, Christian Jacoby, Tobias Jung, Michael Agne, Mario Mergelsberg, Robert Starke, Nico Jehmlich, Martin von Bergen, Hans-Hermann Richnow, Thomas Brüls, Matthias Boll
The denitrifying betaproteobacterium Sterolibacterium denitrificans serves as model organism for studying the oxygen-independent degradation of cholesterol. Here, we demonstrate its capability of degrading various globally abundant side chain containing zoo-, phyto- and mycosterols. We provide the complete genome that empowered an integrated genomics/proteomics/metabolomics approach, accompanied by the characterization of a characteristic enzyme of steroid side chain degradation. The results indicate that individual molybdopterin-containing steroid dehydrogenases are involved in C25-hydroxylations of steroids with different isoprenoid side chains, followed by the unusual conversion to C26-oic acids...
September 21, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28851999/benzo-a-pyrene-induced-metabolic-shift-from-glycolysis-to-pentose-phosphate-pathway-in-the-human-bladder-cancer-cell-line-rt4
#19
Nisha Verma, Mario Pink, Stefan Boland, Albert W Rettenmeier, Simone Schmitz-Spanke
Benzo[a]pyrene (B[a]P), a well-known polyaromatic hydrocarbon, is known for its lung carcinogenicity, however, its role in bladder cancer development is still discussed. Comparative two-dimensional blue native SDS-PAGE analysis of protein complexes isolated from subcellular fractions of 0.5 µM B[a]P-exposed cells indicated a differential regulation of proteins involved in carbohydrate, fatty acid, and nucleotide metabolism, suggesting a possible metabolic flux redistribution. It appeared that B[a]P exposure led to a repression of enzymes (fructose-bisphosphate aldolase A, glucose-6-phosphate isomerase, lactate dehydrogenase) involved in glycolysis, and an up-regulation of proteins (glucose-6-phosphate 1-dehydrogenase, 6-phosphogluconolactonase) catalyzing the pentose phosphate pathway and one carbon metabolism (10-formyltetrahydrofolate dehydrogenase, bifunctional purine biosynthesis protein)...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28792616/proteomic-profiling-of-liver-and-plasma-in-chronic-ethanol-feeding-model-of-hepatic-alcohol-dehydrogenase-deficient-deer-mice
#20
Kamlesh K Bhopale, Samir M Amer, Lata Kaphalia, Kizhake V Soman, John E Wiktorowicz, Ghulam A Shakeel Ansari, Bhupendra S Kaphalia
BACKGROUND: Chronic alcohol abuse, a major risk factor for such diseases as hepatitis and cirrhosis, impairs hepatic alcohol dehydrogenase (ADH; key ethanol [EtOH]-metabolizing enzyme). Therefore, differentially altered hepatic and plasma proteomes were identified in chronic EtOH feeding model of hepatic ADH-deficient (ADH(-) ) deer mice to understand the metabolic basis of alcoholic liver disease (ALD). METHODS: ADH(-) deer mice were fed 3.5 g% EtOH via Lieber-DeCarli liquid diet daily for 3 months and histology of the liver assessed...
October 2017: Alcoholism, Clinical and Experimental Research
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