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Non Enzymatic Glycation

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https://www.readbyqxmd.com/read/28323046/centaurium-erythraea-methanol-extract-protects-red-blood-cells-from-oxidative-damage-in-streptozotocin-induced-diabetic-rats
#1
Miloš Đorđević, Mirjana Mihailović, Jelena Arambašić Jovanović, Nevena Grdović, Aleksandra Uskoković, Anja Tolić, Marija Sinadinović, Jovana Rajić, Danijela Mišić, Branislav Šiler, Goran Poznanović, Melita Vidaković, Svetlana Dinić
ETHNOPHARMACOLOGICAL RELEVANCE: Centaurium erythraea Rafn (CE) is a traditional medicinal herb in Serbia with antidiabetic, digestive, antipyretic and antiflatulent effects. AIM OF THE STUDY: To investigate the potential protective effects of the methanol extract of the aerial parts of CE against glyco-oxidative stress in red blood cells (RBCs) in rats with experimentally induced diabetes. MATERIAL AND METHODS: Diabetes was induced in Wistar rats by intraperitoneal (i...
March 16, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28322944/new-insights-into-non-enzymatic-glycation-of-human-serum-albumin-biopolymer-a-study-to-unveil-its-impaired-structure-and-function
#2
Alok Raghav, Jamal Ahmad, Khursheed Alam, Asad U Khan
Albumin glycation and subsequent formation of advanced glycation end products (AGEs) correlate with diabetes and associated complications. Human Serum Albumin (HSA) was modified with d-glucose for a 40day period under sterile conditions at 37°C. Modified samples along with native HSA (unmodified) were analyzed for impairment in biochemical characteristics ((fructosamine, carbonyl, thiol, lysine, arginine, hydroxymethyl furfural [HMF] content), electrochemical (electrical conductance), simulated gastric fluid assay, simulated intestinal fluid assay, spectroscopic properties (UV, fluorescence), optical (surface contact angle) and fluid dynamics (viscosity and Stokes radius)...
March 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28319002/hba1c-in-relation-to-incident-diabetes-and-diabetes-related-complications-in-non-diabetic-adults-at-baseline
#3
Patricia Anne Metcalf, Cam Kyle, Tim Kenealy, Rod T Jackson
AIMS: We compared the utility of glycated hemoglobin (HbA1c) and oral glucose tolerance (oGTT) in non-diabetic patients for identifying incident diabetes; all-cause mortality; cardiovascular disease (CVD) mortality; CVD, coronary heart disease (CHD), and ischemic stroke events; and diabetes microvascular complications. METHODS: Data from a New Zealand community setting were prospectively linked to hospitalization, mortality, pharmaceutical and laboratory test results data...
February 21, 2017: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/28257177/glycation-of-lysozyme-by-glycolaldehyde-provides-new-mechanistic-insights-in-diabetes-related-protein-aggregation
#4
Laura Mariño, Carlos Andrés Maya-Aguirre, Kris Pauwels, Bartolomé Vilanova, Joaquin Ortega-Castro, Juan Frau, Josefa Donoso, Miquel Adrover
Glycation occurs in vivo as a result of the nonenzymatic reaction of carbohydrates (and/or their autoxidation products) with proteins, DNA, or lipids. Protein glycation causes loss-of-function and, consequently, the development of diabetic-related diseases. Glycation also boosts protein aggregation, which can be directly related with the higher prevalence of aggregating diseases in diabetic people. However, the molecular mechanism connecting glycation with aggregation still remains unclear. Previously we described mechanistically how glycation of hen egg-white lysozyme (HEWL) with ribose induced its aggregation...
March 14, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28230058/macrophage-migration-inhibitory-factor-is-subjected-to-glucose-modification-and-oxidation-in-alzheimer-s-disease
#5
Omar Kassaar, Marta Pereira Morais, Suying Xu, Emily L Adam, Rosemary C Chamberlain, Bryony Jenkins, Tony James, Paul T Francis, Stephen Ward, Robert J Williams, Jean van den Elsen
Glucose and glucose metabolites are able to adversely modify proteins through a non-enzymatic reaction called glycation, which is associated with the pathology of Alzheimer's Disease (AD) and is a characteristic of the hyperglycaemia induced by diabetes. However, the precise protein glycation profile that characterises AD is poorly defined and the molecular link between hyperglycaemia and AD is unknown. In this study, we define an early glycation profile of human brain using fluorescent phenylboronate gel electrophoresis and identify early glycation and oxidation of macrophage migration inhibitory factor (MIF) in AD brain...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28192530/non-enzymatic-glucosylation-induced-neo-epitopes-on-human-serum-albumin-a-concentration-based-study
#6
Km Neelofar, Zarina Arif, Jamal Ahmad, Khursheed Alam
Hyperglycaemia induced non enzymatic glycation is accelerated in diabetic patients and aggressively involved in diabetes progression. Human serum albumin (HSA) is the most abundant protein in blood circulation. In hyperglycaemia, it undergoes fast glycation and results in the impairment of structure. Our previous study has demonstrated structural alterations in Amadori-albumin modified with different glucose concentrations from physiological to pathophysiological range. Here, we focused on immunological characterization of Amadori-albumin...
2017: PloS One
https://www.readbyqxmd.com/read/28192135/formation-mechanism-of-glyoxal-dna-adduct-a-dna-cross-link-precursor
#7
B Vilanova, D Fernández, R Casasnovas, A M Pomar, J R Alvarez-Idaboy, N Hernández-Haro, A Grand, M Adrover, J Donoso, J Frau, F Muñoz, J Ortega-Castro
DNA nucleobases undergo non-enzymatic glycation to nucleobase adducts which can play important roles in vivo. In this work, we conducted a comprehensive experimental and theoretical kinetic study of the mechanisms of formation of glyoxal-guanine adducts over a wide pH range in order to elucidate the molecular basis for the glycation process. Also, we performed molecular dynamics simulations to investigate how open or cyclic glyoxal-guanine adducts can cause structural changes in an oligonucleotide model. A thermodynamic study of other glycating agents including methylglyoxal, acrolein, crotonaldehyde, 4-hydroxynonenal and 3-deoxyglucosone revealed that, at neutral pH, cyclic adducts were more stable than open adducts; at basic pH, however, the open adducts of 3-deoxyglucosone, methylglyoxal and glyoxal were more stable than their cyclic counterparts...
May 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28124608/immunoglobulin-g-glycation-by-fructose-leads-to-structural-perturbations-and-drop-off-in-free-lysine-and-arginine-residues
#8
Mohammad Faisal, Abdulrahman Alatar, Saheem Ahmad
Non-enzymatic glycation is the addition of free carbonyl group of reducing sugar to the free amino groups of proteins and leads to the formation of early glycation products and further into advanced glycation end products (AGEs). Fructose reacts rapidly with the free amino groups of proteins to form AGEs. AGEs are believed to be involved in the pathogenesis of several diseases, particularly in diabetic complications. In this study, IgG was glycated with fructose monosaccharide at 10 mM concentration for varying time interval...
January 17, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28109917/assessment-of-collagen-quality-associated-with-non-enzymatic-cross-links-in-human-bone-using-fourier-transform-infrared-imaging
#9
F N Schmidt, E A Zimmermann, G M Campbell, G E Sroga, K Püschel, M Amling, S Y Tang, D Vashishth, B Busse
Aging and many disease conditions, most notably diabetes, are associated with the accumulation of non-enzymatic cross-links in the bone matrix. The non-enzymatic cross-links, also known as advanced glycation end products (AGEs), occur at the collagen tissue level, where they are associated with reduced plasticity and increased fracture risk. In this study, Fourier-transform infrared (FTIR) imaging was used to detect spectroscopic changes associated with the formation of non-enzymatic cross-links in human bone collagen...
January 18, 2017: Bone
https://www.readbyqxmd.com/read/28013188/a-capture-method-based-on-the-vc1-domain-reveals-new-binding-properties-of-the-human-receptor-for-advanced-glycation-end-products-rage
#10
Genny Degani, Alessandra A Altomare, Mara Colzani, Caterina Martino, Angelica Mazzolari, Guenter Fritz, Giulio Vistoli, Laura Popolo, Giancarlo Aldini
The Advanced Glycation and Lipoxidation End products (AGEs and ALEs) are a heterogeneous class of compounds derived from the non-enzymatic glycation or protein adduction by lipoxidation break-down products. The receptor for AGEs (RAGE) is involved in the progression of chronic diseases based on persistent inflammatory state and oxidative stress. RAGE is a pattern recognition receptor (PRR) and the inhibition of the interaction with its ligands or of the ligand accumulation have a potential therapeutic effect...
April 2017: Redox Biology
https://www.readbyqxmd.com/read/28005306/non-enzymatic-glycation-interferes-with-fibronectin-integrin-interactions-in-vascular-smooth-muscle-cells
#11
Srijita Dhar, Zhe Sun, Gerald A Meininger, Michael A Hill
OBJECTIVE: We aimed to investigate whether advanced non-enzymatic glycation of the extracellular matrix (ECM) protein, fibronectin, impacts its normal integrin-mediated interaction with arteriolar vascular smooth muscle cells (VSMC). METHODS: Atomic force microscopy (AFM) was performed on cultured VSMC from rat cremaster arterioles to study native and glycated fibronectin (FN and gFN) interactions with cellular integrins. AFM probes were functionalized with FN or gFN or with native or glycated albumin (gAlb) as controls...
December 22, 2016: Microcirculation: the Official Journal of the Microcirculatory Society, Inc
https://www.readbyqxmd.com/read/27968979/death-of-embryos-from-2300-year-old-quinoa-seeds-found-in-an-archaeological-site
#12
Hernán Pablo Burrieza, Agustín Sanguinetti, Catalina Teresa Michieli, Héctor Daniel Bertero, Sara Maldonado
In the 1970s, during excavations at Los Morrillos, San Juan, Argentina, quinoa seeds were found within ancient pumpkin crocks protected from the light and high temperatures, and preserved in the very dry conditions of the region. The radiocarbon dates confirmed the age of these seeds at around 2300 years. Sectioning of some of these seeds showed reddish-brown embryos, different from the white embryos of recently harvested quinoa seeds. The ancient seeds did not germinate. The structure of the embryo cells was examined using light and transmission electron microscopy; proteins were analyzed by electrophoresis followed by Coomassie blue and periodic acid Schiff staining and fatty acids by gas chromatography...
December 2016: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/27903233/evaluation-of-structure-chaperone-like-activity-and-allergenicity-of-reduced-glycated-adduct-of-bovine-%C3%AE-casein
#13
Reza Yousefi, Leila Ferdowsi, Zohreh Tavaf, Tanaz Sadeghian, Ali M Tamaddon, Mozhgan Moghtaderi, Zahra Pourpak
Milk has a potent reducing environment with an important quantity of sugar levels. In the current study β-casein was glycated in the presence of D-glucose and sodium cyanoborohydride as a reducing agent. Then, the reduced glucitol adduct of β-casein was used for the structural and functional analyses using different spectroscopic techniques. The results of fluorescence and far ultraviolet circular dichroism assessments suggest important structural alteration upon non-enzymatic glycation of β-casein. In addition, the chaperone activity, micellization properties and antioxidant activity of this protein were altered upon glucose modification...
2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/27827482/glycation-induces-conformational-changes-in-the-amyloid-%C3%AE-peptide-and-enhances-its-aggregation-propensity-molecular-insights
#14
Asis K Jana, Kedar B Batkulwar, Mahesh J Kulkarni, Neelanjana Sengupta
The cytotoxicity of the amyloid beta (Aβ) peptide, implicated in the pathogenesis of Alzheimer's disease (AD), can be enhanced by its post-translational glycation, a series of non-enzymatic reactions with reducing sugars and reactive dicarbonyls. However, little is known about the underlying mechanisms that potentially enhance the cytotoxicity of the advanced glycation modified Aβ. In this work, fully atomistic molecular dynamics (MD) simulations are exploited to obtain direct molecular insights into the process of early Aβ self-assembly in the presence and absence of glycated lysine residues...
November 9, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27816946/advanced-glycation-end-products-mediated-cellular-and-molecular-events-in-the-pathology-of-diabetic-nephropathy
#15
REVIEW
Anil Kumar Pasupulati, P Swathi Chitra, G Bhanuprakash Reddy
Diabetic nephropathy (DN) is a major cause of morbidity and mortality in diabetic patients and a leading cause of end-stage renal disease (ESRD). Degenerative changes such as glomerular hypertrophy, hyperfiltration, widening of basement membranes, tubulointerstitial fibrosis, glomerulosclerosis and podocytopathy manifest in various degrees of proteinuria in DN. One of the key mechanisms implicated in the pathogenesis of DN is non-enzymatic glycation (NEG). NEG is the irreversible attachment of reducing sugars onto free amino groups of proteins by a series of events, which include the formation of Schiff's base and an Amadori product to yield advanced glycation end products (AGEs)...
December 1, 2016: Biomolecular Concepts
https://www.readbyqxmd.com/read/27816054/increased-age-rage-ratio-in-idiopathic-pulmonary-fibrosis
#16
Carlos Machahua, Ana Montes-Worboys, Roger Llatjos, Ignacio Escobar, Jordi Dorca, Maria Molina-Molina, Vanesa Vicens-Zygmunt
BACKGROUND: The abnormal epithelial-mesenchymal restorative capacity in idiopathic pulmonary fibrosis (IPF) has been recently associated with an accelerated aging process as a key point for the altered wound healing. The advanced glycation end-products (AGEs) are the consequence of non-enzymatic reactions between lipid and protein with several oxidants in the aging process. The receptor for AGEs (RAGEs) has been implicated in the lung fibrotic process and the alveolar homeostasis. However, this AGE-RAGE aging pathway has been under-explored in IPF...
November 5, 2016: Respiratory Research
https://www.readbyqxmd.com/read/27796444/effects-of-raloxifene-and-alendronate-on-non-enzymatic-collagen-cross-links-and-bone-strength-in-ovariectomized-rabbits-in-sequential-treatments-after-daily-human-parathyroid-hormone-1-34-administration
#17
S Kimura, M Saito, Y Kida, A Seki, Y Isaka, K Marumo
This study investigated the effects of raloxifene and alendronate to follow parathyroid hormone (PTH) on bone collagen and biomechanical properties in ovariectomized rabbits. Sequential treatments of raloxifene and alendronate after hPTH(1-34) treatment improved biomechanical properties with and without bone collagen improvement, respectively. INTRODUCTION: The standard sequential treatment to follow human parathyroid hormone (hPTH) (1-34) therapy for osteoporosis has yet to be determined...
October 29, 2016: Osteoporosis International
https://www.readbyqxmd.com/read/27787813/the-influence-of-fluorine-on-the-disturbances-of-homeostasis-in-the-central-nervous-system
#18
REVIEW
K Dec, A Łukomska, D Maciejewska, K Jakubczyk, I Baranowska-Bosiacka, D Chlubek, A Wąsik, I Gutowska
Fluorides occur naturally in the environment, the daily exposure of human organism to fluorine mainly depends on the intake of this element with drinking water and it is connected with the geographical region. In some countries, we can observe the endemic fluorosis-the damage of hard and soft tissues caused by the excessive intake of fluorine. Recent studies showed that fluorine is toxic to the central nervous system (CNS). There are several known mechanisms which lead to structural brain damage caused by the excessive intake of fluorine...
October 27, 2016: Biological Trace Element Research
https://www.readbyqxmd.com/read/27774900/phytochemicals-against-advanced-glycation-end-products-ages-and-the-receptor-system
#19
Sho-Ichi Yamagishi, Takanori Matsui, Yuji Ishibashi, Fumiyuki Isami, Yumi Abe, Tatsuya Sakaguchi, Yuichiro Higashimoto
Reducing sugars can react non-enzymatically with amino groups of proteins and lipids to form irreversibly cross-linked macroprotein derivatives called as advanced glycation end products (AGEs). Cross-linking modification of extracellular matrix proteins by AGEs deteriorate their tertiary structural integrity and function, contributing to aging-related organ damage and diabetes-associated complications, such as cardiovascular disease (CVD). Moreover, engagement of receptor for AGEs, RAGE with the ligands evoke oxidative stress generation and inflammatory, thrombotic and fibrotic reactions in various kinds of tissues, further exacerbating the deleterious effects of AGEs on multiple organ systems...
October 21, 2016: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/27751506/advanced-glycation-end-products-an-emerging-biomarker-for-adverse-outcome-in-patients-with-peripheral-artery-disease
#20
Lisanne C de Vos, Joop D Lefrandt, Robin P F Dullaart, Clark J Zeebregts, Andries J Smit
Patients with peripheral artery disease (PAD) suffer from widespread atherosclerosis. Partly due to the growing awareness of cardiovascular disease, the incidence of PAD has increased considerably during the past decade. It is anticipated that algorithms to identify high risk patients for cardiovascular events require being updated, making use of novel biomarkers. Advanced glycation end products (AGEs) are moieties formed non-enzymatically on long-lived proteins under influence of glycemic and oxidative stress reactions...
October 6, 2016: Atherosclerosis
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