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Isolation and characterization of compounds

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https://www.readbyqxmd.com/read/28742929/a-structurally-characterized-cu-iii-complex-supported-by-a-bis-anilido-ligand-and-its-oxidative-catalytic-activity
#1
Fabrice Thomas, Amelie Kochem, Jennifer K Molloy, Gisèle Gellon, Nicolas Leconte, Christian Philouze, Olivier Jarjayes, Florian Berthiol
Three copper(II) complexes of the (R,R)-N,N'-Bis(3,5-di-tert-butyl-2-aminobenzylidene)-1,2-diaminocyclohexane, namely [Cu(NL)], [Cu(NLH)]+ and [Cu(NLH2)]2+, were prepared and structurally characterized. In [Cu(NLH2)]2+ the copper ion lies in an octahedral geometry with the aniline groups coordinated in equatorial positions. In [Cu(NL)] the anilines are deprotonated (anilido moieties) and coordinated to an almost square metal ion. Complex [Cu(NL)] displays two oxidation waves at E1/2ox,1 = -0.14 V and E1/2ox,2 = 0...
July 25, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28741095/phenotype-microarray-analysis-of-the-aders-two-component-system-in-acinetobacter-baumannii
#2
J-R Sun, Y-S Chiang, H-S Shang, C-L Perng, Y-S Yang, T-S Chiueh
Acinetobacter baumannii is a nosocomial pathogen capable of resistance to multiple antimicrobials. The AdeRS two-component system (TCS) is associated with antimicrobial resistance by controlling the AdeABC efflux pump. To elucidate modulation by AdeRS, we made an A. baumannii mutant lacking the AdeRS TCS and characterized it using phenotype microarray (PM) analysis. After disrupting the adeRS operon, lower expression of AdeABC efflux pump was observed in the mutant strain. PM analysis showed that the AdeRS deletion strain and parental strain presented different tolerances to 91 compounds...
July 24, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28740758/identification-and-characterization-of-streptomyces-flavogriseus-nj-4-as-a-novel-producer-of-actinomycin-d-and-holomycin
#3
Zhaohui Wei, Chao Xu, Juan Wang, Fengxia Lu, Xiaomei Bie, Zhaoxin Lu
This paper is the first public report that Streptomyces flavogriseus can produce both actinomycin D and holomycin. The actinomycete strain NJ-4 isolated from the soil of Nanjing Agricultural University was identified as S. flavogriseus. This S. flavogriseus strain was found for the first time to produce two antimicrobial compounds that were identified as actinomycin D and holomycin. GS medium, CS medium and GSS medium were used for the production experiments. All three media supported the production of actinomycin D, while holomycin was detected only in GS medium and was undetectable by HPLC in the CS and GSS media...
2017: PeerJ
https://www.readbyqxmd.com/read/28740604/identification-of-a-novel-trpm8-agonist-from-nutmeg-a-promising-cooling-compound
#4
Tomohiro Shirai, Kentaro Kumihashi, Mitsuyoshi Sakasai, Hiroshi Kusuoku, Yusuke Shibuya, Atsushi Ohuchi
The transient receptor potential melastatin 8 (TRPM8) ion channel is the primary receptor for innocuous cold stimuli (<28 °C) in humans. TRPM8 agonists such as l-(-)-menthol are widely used as flavors and additives to impart briskness, in addition to medicinal uses for inflammation and pain. Though various natural and synthetic agonists have been explored, only few natural compounds are known. We report herein the identification and characterization of the novel neolignan agonist erythro- and threo-Δ(8')-7-ethoxy-4-hydroxy-3,3',5'-trimethoxy-8-O-4'-neolignan (1) with an EC50 of 0...
July 13, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28739549/mutations-in-the-abcg8-gene-are-associated-with-sitosterolaemia-in-the-homozygous-form-and-xanthelasmas-in-the-heterozygous-form
#5
Tara Bardawil, Abdallah Rebeiz, Myriam Chaabouni, Jessica El Halabi, Zakaria Kambris, Ossama Abbas, Ossama Abou Hassan, Lamiaa Hamie, Fadi Bitar, Abdul Ghani Kibbi, Georges Nemer, Mazen Kurban
Sitosterol is the most abundant plant sterol found in our diet. Sitosterolemia (OMIM 210250), also known as phytosterolaemia, is a rare autosomal recessive disease caused by the inability to efficiently excrete plant sterol, and is characterized by cutaneous xanthomas and accelerated atherosclerosis. Sitosterolaemia is caused by homozygous or compound heterozygous mutations in either ABCG5 or ABCG8 (both on chromosome 2p21), which encode the sterol efflux transporter ABCG5 (sterolin-1) and ABCG8 (sterolin-2), respectively...
July 25, 2017: European Journal of Dermatology: EJD
https://www.readbyqxmd.com/read/28738515/characterization-of-carbon-dioxide-concentrating-chemolithotrophic-bacterium-serratia-sp-istd04-for-production-of-biodiesel
#6
Manish Kumar, Raj Morya, Edgard Gnansounou, Christian Larroche, Indu Shekhar Thakur
Proteomics and metabolomics analysis has become a powerful tool for characterization of microbial ability for fixation of Carbon dioxide. Bacterial community of palaeoproterozoic metasediments was enriched in the shake flask culture in the presence of NaHCO3. One of the isolate showed resistance to NaHCO3 (100mM) and was identified as Serratia sp. ISTD04 by 16S rRNA sequence analysis. Carbon dioxide fixing ability of the bacterium was established by carbonic anhydrase enzyme assay along with proteomic analysis by LC-MS/MS...
July 14, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28738409/elements-of-eoarchean-life-trapped-in-mineral-inclusions
#7
T Hassenkam, M P Andersson, K N Dalby, D M A Mackenzie, M T Rosing
Metasedimentary rocks from Isua, West Greenland (over 3,700 million years old) contain (13)C-depleted carbonaceous compounds, with isotopic ratios that are compatible with a biogenic origin. Metamorphic garnet crystals in these rocks contain trails of carbonaceous inclusions that are contiguous with carbon-rich sedimentary beds in the host rock, where carbon is fully graphitized. Previous studies have not been able to document other elements of life (mainly hydrogen, oxygen, nitrogen and phosphorus) structurally bound to this carbonaceous material...
July 24, 2017: Nature
https://www.readbyqxmd.com/read/28738194/characterization-and-horizontal-transfer-of-qach-associated-class-1-integrons-in-escherichia-coli-isolated-from-retail-meats
#8
Xiaobing Jiang, Yameng Xu, Yi Li, Kun Zhang, Lei Liu, Hailei Wang, Jinhe Tian, Hao Ying, Lei Shi, Tao Yu
The aim of this study was to examine the presence of genes responsible for resistance to quaternary ammonium compounds (QACs) and the association of qac genes with class 1 integrons in Escherichia coli isolated from retail meats. Among the 179 E. coli isolates tested, the minimum inhibitory concentrations (MICs) of benzalkonium chloride (BC) ranged from 4 to 64μg/mL. PCR assays indicated that QAC-resistance genes sugE(c), ydgE/ydgF, mdfA, emrE and qacEΔ1 were commonly present (40.2%-88.3%) in these isolates, but qacE, qacF, qacH and sugE(p) were less prevalent (2...
July 15, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28734965/enhanced-immunostimulatory-activity-of-in-silico-discovered-agonists-of-toll-like-receptor-2-tlr2
#9
M S Murgueitio, S Ebner, P Hörtnagl, C Rakers, R Bruckner, P Henneke, G Wolber, S Santos-Sierra
BACKGROUND: Emergent therapies in anticancer vaccination use Toll-like receptors (TLRs) agonists as dendritic cell (DC) vaccine adjuvants. DCs from the patient are isolated, stimulated with TLR agonists and tumor antigens ex vivo and then infused back into the patient. Although some TLR ligands have been tested in clinical trials, novel TLR agonists with improved immunomodulatory properties are essential to optimize treatment success. We report on the discovery of small-molecule TLR2 agonists, with favorable properties as synthetic adjuvants...
July 19, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28734932/a-pyridone-derivative-activates-serca2a-by-attenuating-the-inhibitory-effect-of-phospholamban
#10
Manami Kaneko, Hisato Yamamoto, Hiroki Sakai, Yusuke Kamada, Toshiki Tanaka, Shuji Fujiwara, Synsuke Yamamoto, Hiroki Takahagi, Hideyuki Igawa, Shizuo Kasai, Masakuni Noda, Makoto Inui, Tomoyuki Nishimoto
The cardiac sarco/endoplasmic reticulum Ca(2+)-dependent ATPase 2a (SERCA2a) plays a central role in Ca(2+) handling within cardiomyocytes and is negatively regulated by phospholamban (PLN), a sarcoplasmic reticulum (SR) membrane protein. The activation of SERCA2a, which has been reported to improve cardiac dysfunction in heart failure, is a potential therapeutic approach for heart failure. Therefore, we developed a novel small molecule, compound A and characterized it both in vitro and in vivo. Compound A activated the Ca(2+)-dependent ATPase activity of cardiac SR vesicles but not that of skeletal muscle SR vesicles that lack PLN...
July 19, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28733286/the-regulator-ldhr-and-the-d-lactate-dehydrogenase-ldha-of-burkholderia-multivorans-play-a-role-in-carbon-overflow-and-in-planktonic-cellular-aggregates-formation
#11
Inês N Silva, Marcelo J Ramires, Lisa A Azevedo, Ana R Guerreiro, Andreia C Tavares, Jörg D Becker, Leonilde M Moreira
LysR-type transcriptional regulators (LTTR) are the most commonly found regulators in Burkholderia cepacia complex, comprising opportunistic pathogens causing chronic respiratory infections in cystic fibrosis (CF) patients. Despite LTTRs being global regulators of pathogenicity in several bacteria, few have been characterized in Burkholderia Here, we showed that gene ldhR of B. multivorans encoding a LTTR is co-transcribed with ldhA encoding a d-lactate dehydrogenase, and evaluate their implication in virulence traits like exopolysaccharide (EPS) synthesis and biofilm formation...
July 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28730389/cardiovascular-effects-of-high-molecular-weight-compounds-of-humic-nature
#12
M V Zykova, M V Belousov, T V Lasukova, A S Gorbunov, L A Logvinova, A M Dygai
The active ingredient extracted from the peat humic substances was characterized by physicochemical parameters evaluated by UV- and IR-spectroscopy, titration, and elemental (C, H, N) analysis. The cardiovascular effects of this ingredient were examined on isolated Langendorff-perfused rat heart. It was found that the active substance in a concentration range of 0.01-0.1 mg/ml produced a vasodilating effect; in addition, it decreased the end-diastolic and left-ventricular developed pressures.
July 18, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28729705/diterpenoid-alkaloids-from-delphinium-anthriscifolium-var-majus
#13
Lian-Hai Shan, Ji-Fa Zhang, Feng Gao, Shuai Huang, Xian-Li Zhou
Extensive phytochemical investigation on the whole herbs of Delphinium anthriscifolium var. majus led to the identification of fourteen diterpenoid alkaloids, including three new C20-diterpenoid alkaloids (anthriscifolsines A-C, 1-3), six new C19-diterpenoid alkaloids (anthriscifolrines A-F, 4-9), and five know compounds (10-14). Among them, anthriscifolsine A represents a novel C20-diterpenoid alkaloid characterized by a seco C-ring. The structures of the isolated compounds were elucidated by extensive spectroscopic methods, including HR-ESI-MS, X-ray, and 1D and 2D NMR experiments...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729041/pharmacological-preclinical-characterization-of-las190792-a-novel-inhaled-bifunctional-muscarinic-receptor-antagonist-%C3%AE-2-adrenoceptor-agonist-maba-molecule
#14
Mònica Aparici, Carla Carcasona, Israel Ramos, José Luís Montero, José Luís Ortiz, Julio Cortijo, Carlos Puig, Dolors Vilella, Chris Doe, Amadeu Gavaldà, Montserrat Miralpeix
LAS190792 is a novel muscarinic antagonist and β2-adrenoceptor agonist in development for chronic respiratory diseases. This study investigated the pharmacological profile of LAS190792 in comparison to batefenterol, tiotropium, indacaterol and olodaterol. LAS190792 is potent at the human M3 receptor (pIC50: 8.8 in binding assays). It is selective for the β2-adrenoceptor over the β1-and β3-adrenoceptor, and shows a functional potency in a similar range to batefenterol and LABA compounds (pEC50 in spontaneous tone isolated trachea: 9...
July 17, 2017: Pulmonary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28728544/cladosporol-a-triggers-apoptosis-sensitivity-by-ros-mediated-autophagic-flux-in-human-breast-cancer-cells
#15
Mytre Koul, Ashok Kumar, Ramesh Deshidi, Vishal Sharma, Rachna D Singh, Jasvinder Singh, Parduman Raj Sharma, Bhawal Ali Shah, Sundeep Jaglan, Shashank Singh
BACKGROUND: Endophytes have proven to be an invaluable resource of chemically diverse secondary metabolites that act as excellent lead compounds for anticancer drug discovery. Here we report the promising cytotoxic effects of Cladosporol A (HPLC purified >98%) isolated from endophytic fungus Cladosporium cladosporioides collected from Datura innoxia. Cladosporol A was subjected to in vitro cytotoxicity assay against NCI60 panel of human cancer cells using MTT assay. We further investigated the molecular mechanism(s) of Cladosporol A induced cell death in human breast (MCF-7) cancer cells...
July 20, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28727774/inhibitors-of-mycobacterium-marinum-virulence-identified-in-a-dictyostelium-discoideum-host-model
#16
Hajer Ouertatani-Sakouhi, Sébastien Kicka, Gianpaolo Chiriano, Christopher F Harrison, Hubert Hilbi, Leonardo Scapozza, Thierry Soldati, Pierre Cosson
Tuberculosis remains one of the major threats to public health worldwide. Given the prevalence of multi drug resistance (MDR) in Mycobacterium tuberculosis strains, there is a strong need to develop new anti-mycobacterial drugs with modes of action distinct from classical antibiotics. Inhibitors of mycobacterial virulence might target new molecular processes and may represent a potential new therapeutic alternative. In this study, we used a Dictyostelium discoideum host model to assess virulence of Mycobacterium marinum and to identify compounds inhibiting mycobacterial virulence...
2017: PloS One
https://www.readbyqxmd.com/read/28725623/isolation-and-characterization-of-antimicrobial-compound-from-the-stem-bark-of-the-traditionally-used-medicinal-plant-adenium-obesum
#17
Mohammed Sohail Akhtar, Mohammad Amzad Hossain, Sadri Abdullah Said
BACKGROUND: Medicinal plants constitute a natural reservoir for medicines worldwide. They serve mainstream therapeutics and are central in folklore medicine. In case of Adenium obesum (Lav, Apocynaceae), indigenous people of Oman use it for the treatment of venereal diseases, wounds, skin diseases, headaches, muscle pain as well as joint pain; yet, the active ingredients have not been classified. To screened the antioxidant and antimicrobial activities of an identified compound that we isolated from the highest active chloroform extract...
July 2017: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/28725154/new-constituents-triterpene-ester-and-sugar-derivatives-from-panax-ginseng-meyer-and-their-evaluation-of-antioxidant-activities
#18
Ill-Min Chung, Nasir Ali Siddiqui, Seung Hyun Kim, Praveen Nagella, Azmat Ali Khan, Mohd Ali, Ateeque Ahmad
Panax ginseng C. A. Meyer (Araliaceae), is a well-known herb and used in the old established system of Oriental remedy, especially in Japan, China and Korea. Four new compounds characterized as (cis)- 7β,11α,19,21-tetra-O-decanoyl-18, 22β-dihydroxy-dammar-1-en-3-one (1), 3β,4α,12β-trihydroxystigmast-5-en-21-yl octadecan-9',12'-dienoate (2), dammar-12, 24-dien-3α, 6β, 15α-triol-3α-D-arabinopyranosyl-6β-L-arabinopyranoside (3) and dammar-24-en-3α, 6β, 16α, 20β-tetraol-3α-D-arabinopyranosyl-6β-D-arabinopyranoside (4) were isolated and established from the ethyl acetate and butanol extracts of the roots of P...
July 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28722076/the-anomalous-halogen-bonding-interactions-between-chlorine-and-bromine-with-water-in-clathrate-hydrates
#19
Hana Dureckova, Tom K Woo, Konstantin A Udachin, John A Ripmeester, Saman Alavi
Clathrate hydrate phases of Cl2 and Br2 guest molecules have been known for about 200 years. The crystal structure of these phases was recently re-determined with high accuracy by single crystal X-ray diffraction. In these structures, the water oxygen-halogen atom distances are determined to be shorter than the sum of the van der Waals radii, which indicates the action of some type of non-covalent interaction between the dihalogens and water molecules. Given that in the hydrate phases both lone pairs of each water oxygen atom are engaged in hydrogen bonding with other water molecules of the lattice, the nature of the oxygen-halogen interactions may not be the standard halogen bonds characterized recently in the solid state materials and enzyme-substrate compounds...
July 19, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28721507/the-diversity-and-antibiotic-properties-of-actinobacteria-associated-with-endemic-deepwater-amphipods-of-lake-baikal
#20
Eugenii S Protasov, Denis V Axenov-Gribanov, Yuriy V Rebets, Irina V Voytsekhovskaya, Bogdan T Tokovenko, Zhanna M Shatilina, Andriy N Luzhetskyy, Maxim A Timofeyev
The emergence of pathogenic bacteria resistant to antibiotics increases the need for discovery of new effective antimicrobials. Unique habitats such as marine deposits, wetlands and caves or unexplored biological communities are promising sources for the isolation of actinobacteria, which are among the major antibiotic producers. The present study aimed at examining cultivated actinobacteria strains associated with endemic Lake Baikal deepwater amphipods and estimating their antibiotic activity. We isolated 42 actinobacterial strains from crustaceans belonging to Ommatogammarus albinus and Ommatogammarus flavus...
July 18, 2017: Antonie Van Leeuwenhoek
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