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Isolation and characterization of compounds

Wei Yan, Wei Wei, Yang-Yang Zhang, Jia-Jie Wang, Xiao-Fei Ping, Jia-Yun Xu, Yong-Hao Ye
Two new cyclohexene derivatives, nigrosporanenes C and D (1 and 2), together with three known compounds (3-5), were isolated from the culture of an endophyte Nigrospora oryzae S4. Their structures were characterized by a combination of detailed spectroscopic analysis and comparison of their NMR data with those reported in the literature. All compounds were tested for anti-phytopathogenic activity, however, none of them showed activity at a concentration of 20 μM.
January 15, 2018: Journal of Asian Natural Products Research
Helen L Alvarado, Ana C Calpena, Maria L Garduno-Ramirez, Raul Ortiz, Consolacion Melguizo, Jose M Prados, Beatriz Clares
BACKGROUND: Triterpenoids are an important class of natural bioactive products present in many medicinal plants. OBJECTIVE: The aim of present study is to investigate the antioxidant and anticarcinogenic potential of oleanolic acid (OA) and ursolic acid (UA) on B16 murine melanoma cell line isolated from Plumeria obtusa, free and loaded in a nanoemulsion (NEm) system. METHOD: The nanoemulsion was characterized by dynamic light scattering, transmission electron microscopy...
January 11, 2018: Anti-cancer Agents in Medicinal Chemistry
Daniel J Tindall, Christophe Werlé, Richard Goddard, Petra Philipps, Christophe Farès, Alois Fürstner
Traditional rhodium carbene chemistry relies on the controlled decomposition of diazo derivatives with [Rh2(OAc)4] or related dinuclear Rh(+2) complexes, whereas the use of other rhodium sources is much less developed. It is now shown that half-sandwich carbene species derived from [Cp*MX2]2 (M = Rh, Ir; X = Cl, Br, I, Cp* = pentamethylcyclopentadienyl) also exhibit favorable application profiles. Interestingly, the anionic ligand X proved to be a critical determinant of reactivity in the case of cyclopropanation, epoxide formation and the previously unknown catalytic metathesis of azobenzene derivatives, whereas the nature of X does not play any significant role in -OH insertion reactions...
January 14, 2018: Journal of the American Chemical Society
Abolfazl Shakeri, Milena Masullo, Gilda D'Urso, Mehrdad Iranshahi, Paola Montoro, Cosimo Pizza, Sonia Piacente
Chemical investigations on Glycyrrhiza spp. have mostly been focused on G. glabra (typically cultivated in Europe, henceforth called European licorice), G. uralensis and G. inflata (known as Chinese licorice) with little information on the constituents of other Glycyrrhiza species. According to the growing interest in further Glycyrrhiza spp. to be used as sweeteners, the roots of G. triphylla have been investigated. The LC-ESI/LTQOrbitrap/MS profile of the methanolic extract of G. triphylla roots guided the isolation of 21 compounds, of which the structures were elucidated by 1D- and 2D-NMR experiments...
May 15, 2018: Food Chemistry
Guo-Quan Wang, Jun-Feng Chen, Bo Yi, He-Xin Tan, Lei Zhang, Wan-Sheng Chen
Salvia miltiorrhiza is a medicinal plant widely used in the treatment of cardiovascular and cerebrovascular diseases. Hydrophilic phenolic acids, including rosmarinic acid (RA) and lithospermic acid B (LAB), are its primary medicinal ingredients. However, the biosynthetic pathway of RA and LAB in S. miltiorrhiza is still poorly understood. In the present study, we accomplished the isolation and characterization of a novel S. miltiorrhiza Hydroxyphenylpyruvate reductase (HPPR) gene, SmHPPR, which plays an important role in the biosynthesis of RA...
December 2017: Chinese Journal of Natural Medicines
Abhijit Nandi, Sharath V Sagar, Gajanan Chigure, Ashutosh Fular, Anil Kumar Sharma, Gaurav Nagar, Sachin Kumar, B C Saravanan, Srikant Ghosh
Rhipicephalus microplus, the major cattle tick species of India is prevalent all over the country and causes huge economic loss directly or indirectly to the dairy industries. Chemical acaricides are playing an important role in managing tick infestations on livestock for many years and consequently, resistance to commonly used organophosphate (OP) and synthetic pyrethroid (SP) compounds has been reported. Subsequently, ivermectin (IVM) has been emerged as an alternative to manage OP and SP resistant ticks...
January 30, 2018: Veterinary Parasitology
Babita Sharma, Arun Kumar Dangi, Pratyoosh Shukla
The persistent disposal of xenobiotic compounds like insecticides, pesticides, fertilizers, plastics and other hydrocarbon containing substances is the major source of environmental pollution which needs to be eliminated. Many contemporary remediation methods such as physical, chemical and biological are currently being used, but they are not sufficient to clean the environment. The enzyme based bioremediation is an easy, quick, eco-friendly and socially acceptable approach used for the bioremediation of these recalcitrant xenobiotic compounds from the natural environment...
January 9, 2018: Journal of Environmental Management
Benjamin M Wolf, Christoph Stuhl, Cäcilia Maichle-Mössmer, Reiner Anwander
The salt metathesis reaction between homoleptic calcium bis(trimethylsilyl)amide [Ca{N(SiMe3)2}2]2 and "halide-free" methyllithium MeLi allowed for the isolation of X-ray amorphous dimethylcalcium [CaMe2]n in good yields and purities. The formation of [CaMe2]n was proven by microanalysis and NMR/FTIR spectroscopic methods as well as a series of derivatization reactions. Although slowly decomposing thf, [CaMe2]n could be crystallized from chilled thf solutions as the heptametallic adduct [(thf)10Ca7Me14]. Reaction of [CaMe2]n with CaI2 in thf led to the dimeric complex [(thf)3Ca(Me)(I)]2 whereas in tetrahydropyran (thp) the trinuclear complex [(thp)5Ca3(Me)5(I)] was obtained, both representing the first crystallographically characterized heavy-Grignard compounds with methyl groups as the hydrocarbyl ligand...
January 12, 2018: Journal of the American Chemical Society
Vladimir Morocho, Andrea Valle, Jessica García, Gianluca Gilardoni, Luis Cartuche, Alírica I Suárez
The phytochemical investigation of both volatile and fixed metabolites of Clinopodium taxifolium (Kunth) Govaerts (Lamiaceae) was performed for the first time. It allowed the isolation and characterization of the essential oil and six known compounds: carvacrol (1), squalane (2), uvaol (3), erythrodiol (4), ursolic acid (5), and salvigenin (6). Their structures were identified and characterized by Nuclear Magnetic Resonance (NMR) and Gas Chromatography coupled to Mass Spectroscopy (GC-MS), and corroborated by literature...
January 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Filip Boratyński, Agata Janik-Polanowicz, Ewa Szczepańska, Teresa Olejniczak
Lactone 2a of a bicyclo[4.3.0]nonane structure is a good starting material for synthesis of many attractive compounds. Enantiomerically enriched (-)-(3aR,7aS)-lactone 2a is produced by whole cells of bacteria. In order to examine the impact of the absolute configuration on biological activity we evaluated the process affording the opposite isomer. To this purpose Candida pelliculosa ZP22 characterized by high dehydrogenase activity was used. The goal of presented work was to perform bioreactor scale microbial one-pot oxidation of diol with selected yeast strain C...
January 11, 2018: Scientific Reports
Khadidja Aya Beladjila, Djemaa Berrehal, Nunziatina De Tommasi, Carlotta Granchi, Giulia Bononi, Alessandra Braca, Marinella De Leo
Four new phenylethanoid glycosides (1: -4: ), 1-β-p-hydroxyphenyl-ethyl-2-O-acetyl-3,6-di-α-l-rhamnopyranosyl-β-d-glucopyranoside (1: ), 1-β-p-hydroxyphenyl-ethyl-3,6-O-di-α-l-rhamnopyranosyl-β-d-glucopyranoside (2: ), 1-β-p-hydroxyphenyl-ethyl-2-O-acetyl-3,6-di-α-l-rhamnopyranosyl-4-p-coumaroyl-β-d-glucopyranoside (3: ), and 1-β-p-hydroxyphenyl-ethyl-3,6-di-α-l-rhamnopyranosyl-4-p-coumaroyl-β-d-glucopyranoside (4: ), together with three known compounds, were isolated from the n-butanol extract of Cistanche phelypaea aerial parts...
January 10, 2018: Planta Medica
Adelina Orellana, Vicente García-González, Rosa López, Sonia Pascual-Guiral, Estrella Lozoya, Julia Díaz, Daniel Casals, Antolín Barrena, Stephane Paris, Miriam Andrés, Victor Segarra, Dolors Vilella, Rajneesh Malhotra, Paul Eastwood, Anna Planagumà, Montserrat Miralpeix, Arsenio Nueda
Thymic stromal lymphopoietin (TSLP) is a cytokine released by human lung epithelium in response to external insult. Considered as a master switch in T helper 2 lymphocyte (Th2) mediated responses, TSLP is believed to play a key role in allergic diseases including asthma. The aim of this study was to use a phenotypic approach to identify new biological and chemical starting points for inhibition of TSLP production in human bronchial epithelial cells (NHBE), with the objective of reducing Th2-mediated airway inflammation...
2018: PloS One
Jacek Lubczak, Renata Lubczak, Iwona Zarzyka
The one-pot multicomponent synthesis of oligoetherols containing azacycles is described. They were obtained by reaction of isocyanuric, barbituric, or uric acid or melamine with glycidol and alkylene carbonates. The isolated products were characterized by physical methods and their properties were compared with the same compounds obtained in twostep protocol. The oligoetherols with 1,3,5-triazine ring obtained by both methods were then used to form polyurethane foams and their properties were compared.
December 2017: Acta Chimica Slovenica
Daniel Wilson, Alexander Hinz, Jose Manuel Goicoechea
The synthesis and characterization of a stable phosphaethynolato-borane, [B]OCP (1, [B] = N,N'-bis(2,6-diisopropylphenyl)-2,3-dihydro-1H-1,3,2-diazaboryl), is described. The increased triple bond character of the P-C bond in 1 relative to the free ion (PCO-) is probed in a series of reactivity studies. Compound 1 readily dimerises in donor solvents to afford a cyclic five-membered 6π-aromatic compound, cyclo-P2{C[B]}O{CO[B]} (2), which decarbonylates on UV irradiation. By contrast the nickel-mediated dimerisation of 1 affords the isomeric diphosphacyclobutene [P(CO[B])]2...
January 8, 2018: Angewandte Chemie
Xuexiao Cao, Meng Wang, Lili Sun, Xiaoliang Ren, Gaosheng Pei
BACKGROUND: The highly selective capture of individual components of complex systems such as Chinese medicine extracts is a great challenge. With the rapid development of nanoscience, magnetic materials have attracted increased attention as suitable candidates for use in drug transport vehicles. RESULTS: In this work, amino-modified Fe3 O4 nanoparticles (MNP-NH2 ) have been synthesized and successfully used to selectively isolate Luteolin, a type of flavonoid, from peanut shell, coupled with high-performance liquid chromatography (HPLC)...
January 9, 2018: Journal of the Science of Food and Agriculture
Giorgia Dotto, Michele Berlanda, Daniela Pasotto, Alessandra Mondin, Giorgio Zambotto, Maria Luisa Menandro
Enterococci are important opportunistic pathogens for humans and animals and have recently become one of the leading causes of nosocomial infections, raising concerns about their virulence and antimicrobial traits. This study describes a multidrug-resistant Enterococcus faecium isolated from a case of feline urinary tract infection. This strain was characterized for virulence and antimicrobial resistance markers, phylogenetic group and sensitivity to antimicrobial agents used routinely in veterinary and human practice...
January 9, 2018: New Microbiologica
Jie Xin Tong, Rajesh Chandramohanadas, Kevin Shyong-Wei Tan
Plasmodium falciparum infections leading to malaria have severe clinical manifestations and high mortality rates. Chloroquine (CQ), a former mainstay of malaria chemotherapy, has been rendered ineffective due to the emergence of wide-spread resistance. Recent studies, however, have unveiled a novel mode of action in which low micromolar levels of CQ permeabilized the parasite's digestive vacuole (DV) membrane, leading to calcium efflux, mitochondrial depolarization and DNA degradation. These phenotypes implicate the DV as an alternative target of CQ and suggests that DV disruption is an attractive target for exploitation through the screening for DV-disruptive antimalarials...
January 8, 2018: Antimicrobial Agents and Chemotherapy
Fengyun Liao, Yingjiao Meng, Huazhu Zheng, Dongmei He, Xiao Shen, Jianye Yu, Yinai Wu, Lisheng Wang
Buyang Huanwu decoction (BHD) was reported to exert angiogenesis-promoting effects, but its active ingredients remain unknown. In this study, we developed a method to screen potential angiogenesis-promoting compounds in BHD, which involved biospecific isolation using live rat brain microvascular endothelial cells (rBMECs) and characterization using solid-phase extraction (SPE) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Six compounds showed binding affinity to rBMECs and were further identified as 6-hydroxykaempferol-di-O-glucoside, paeoniflorin, calycosin-7-O-β-D-glucoside, galloylpaeoniflorin, formononetin-7-O-β-D-glucoside, and (3R)-7,2'-hydroxy-3',4'-dimethoxy-isoflavan...
March 1, 2018: Talanta
Hamza Fadel, Ines Sifaoui, Atteneri López-Arencibia, María Reyes-Batlle, Soumaya Hajaji, Olfa Chiboub, Ignacio A Jiménez, Isabel L Bazzocchi, Jacob Lorenzo-Morales, Samir Benayache, José E Piñero
The lack of an effective chemotherapy for treatment of protozoan disease urges a wide investigation for active compounds, and plant-derived compounds continue to provide key leads for therapeutic agents. The current study reports the in vitro antiprotozoal evaluation of the Algerian medicinal plant Pulicaria inuloides against Leishmania amazonensis, Trypanosoma cruzi, and Acanthamoeba castellanii str. Neff. All the extracts from the aerial part showed to be present a higher leishmanicidal activity than anti-Acanthamoeba or Trypanosoma...
January 6, 2018: Parasitology Research
Zuo Peng Li, Yeong Hun Song, Zia Uddin, Yan Wang, Ki Hun Park
Cratoxylum cochinchinense displayed significant inhibition against protein tyrosine phosphatase 1B (PTP1B) and α-glucosidase, both of which are key target enzymes to attenuate diabetes and obesity. The compounds responsible for both enzymes inhibition were identified as twelve xanthones (1-12) among which compounds 1 and 2 were found to be new ones. All of them simultaneously inhibited PTP1B with IC50s of (2.4-52.5 µM), and α-glucosidase with IC50 values of (1.7-72.7 µM), respectively. Cratoxanthone A (3) and γ-mangostin (7) were estimated to be most active inhibitors against both PTP1B (IC50 = 2...
December 26, 2017: Bioorganic & Medicinal Chemistry
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