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Isolation and characterization of compounds

Hana Horváthová, Katarína Lászlová, Katarína Dercová
Elimination of dangerous toxic and hydrophobic chlorinated aromatic compounds, mainly PCBs from the environment, is one of the most important aims of the environmental biotechnologies. In this work, biodegradation of an industrial mixture of PCBs (Delor 103, equivalent to Aroclor 1242) was performed using bacterial consortia composed of four bacterial strains isolated from the historically PCB-contaminated sediments and characterized as Achromobacter xylosoxidans, Stenotrophomonas maltophilia, Ochrobactrum anthropi and Rhodococcus ruber...
November 3, 2017: Chemosphere
Rafael P Pinheiro, Muiara A Moraes, Bruna C S Santos, Rodrigo L Fabri, Glauciemar Del-Vechio-Vieira, Célia H Yamamoto, Ana Lúcia S M Araújo, Aílson L A Araújo, Orlando V Sousa
Palicourea rigida Kunth is traditionally used for the treatment of skin diseases, kidney pains and ovarian inflammation. Based on these traditional uses, this study evaluated the topical anti-inflammatory activity of the ethanol extract from P. rigida leaves (EEPR) and identified bioactive compounds. Ear edema was induced in Swiss mice by the topical application of Croton oil, arachidonic acid, phenol and capsaicin. Histopathological analysis and myeloperoxidase and N-acetyl-β-D-glucosaminidase activities were determined...
November 14, 2017: Inflammopharmacology
Brandon J H Banaschewski, Brandon Baer, Christina Arsenault, Teah Jazey, Edwin J A Veldhuizen, Johan Delport, Tracey Gooyers, James F Lewis, Henk P Haagsman, Ruud A W Veldhuizen, Cory Yamashita
Cystic fibrosis (CF) is characterized by recurrent airway infections with antibiotic-resistant bacteria and chronic inflammation. Chicken cathelicin-2 (CATH-2) has been shown to exhibit antimicrobial activity against antibiotic-resistant bacteria and to reduce inflammation. In addition, exogenous pulmonary surfactant has been suggested to enhance pulmonary drug delivery. It was hypothesized that CATH-2 when combined with an exogenous surfactant delivery vehicle, bovine lipid extract surfactant (BLES), would exhibit antimicrobial activity against CF-derived bacteria and downregulate inflammation...
November 14, 2017: Scientific Reports
Reid E Messersmith, Sangeeta Yadav, Maxime A Siegler, Henrik Ottosson, John D Tovar
This report documents the synthesis, characterization and computational evaluation of two isomeric borepin-containing polycyclic aromatics. The syntheses of these two isomers involved symmetrical disubstituted alkynes that were reduced to Z-olefins followed by borepin formation either through an isolable stannocycle intermediate or directly from the alkene via the trapping of a transient dilithio intermediate. Comparisons of their magnetic, crystallographic and computational characterization to literature compounds gave valuable insights about the aromaticity of these symmetrically fused [b,f]borepins...
November 14, 2017: Journal of Organic Chemistry
Zhiqiang Wang, Seung Hwan Hwang, Soon Sung Lim
In the present study, a novel hyphenation of ultrafiltration (UF), high-speed countercurrent chromatography (HSCCC), and high-performance liquid chromatography (HPLC) was developed for comprehensive profiling and characterization of the minor tyrosinase inhibitors from Gastrodia elata (GE). A small quantity of GE extract was first fractionated by HSCCC, using elution solvents with a wide range of polarities to enrich minor compounds; then, the fractions were profiled by UF-HPLC to generate a comprehensive 2D chromatogram of the distribution of bioactive components...
November 6, 2017: Journal of Chromatography. A
Marisa Jádna Silva Frederico, Allisson Jhonatan Gomes Castro, Veronica Aiceles de Medeiros Pinto, Cristiane da Fonte Ramos, Fabíola Branco Filippin Monteiro, Alessandra Mascarello, Ricardo José Nunes, Fátima Regina Mena Barreto Silva
The aim of the present study was to investigate the mechanism of action of a sulfonamide derivative on glucose uptake in adipose tissue, as well as to characterize the effects of this compound on intestinal disaccharidases and advanced glycation end-products (AGEs) formation. Camphoryl-benzene sulfonamide (CS) was able to stimulate glucose uptake in isolated adipocytes, adipose tissue and in soleus muscle. The stimulatory effect of the compound (10 μM) on glucose uptake on adipose tissue was blocked by diazoxide, wortmannin, U73122, colchicine, and N-ethylmaleimide...
November 11, 2017: Journal of Cellular Biochemistry
Chunshun Li, Ariel M Sarotti, James Turkson, Shugeng Cao
A new secondary metabolite verbenanone (1) with a unique (4aS,8aS)-octahydro-5H-chromen-5-one moiety has been obtained from the endophytic fungus FT431, which was isolated from the native Hawaiian plant Verbena sp. The structure of compound 1 was characterized based on NMR and MS spectroscopic analysis. The absolute configuration (AC) of compound 1 was determined by Mosher acids. Compound 1 was tested against A2780 and A2780cisR, but it was inactive.
June 7, 2017: Tetrahedron Letters
Seong-Bum Park, Jong Youn Kim, Jung Yeon Han, Chang-Ho Ahn, Eung-Jun Park, Yong Eui Choi
Benzoic acids (BAs) are important structural elements in a wide variety of essential compounds and natural products, and play crucial roles in plant fitness. BA is a precursor of diverse benzenoid compounds, including the hormone salicylic acid (SA) and the aglycone moiety of salicin, which is particularly important in the Salicaceae family. The biosynthetic pathways leading to BA formation in plants are largely unknown. Recently, the CoA-dependent β-oxidative BA biosynthesis pathway, which occurs in peroxisomes, has been characterized in petunia...
November 11, 2017: Journal of Chemical Ecology
Lyvia Lintzmaier Petiz, Amanda do Rocio Andrade Pires, Aurea Echevarria, Cláudio Eduardo Rodrigues-Santos, Maria Eliane Merlin Rocha, Alexandra Acco, Silvia Maria Suter Correia Cadena
Amidines are chemically characterized by the presence of two nitrogen atoms that bind to the same carbon atom in its structure. Several biological activities have been ascribed to these compounds. Pentamidine, an aromatic diamidine, is effective in the treatment against Pneumocystis carinii and leishmaniasis, but it can also have severe side effects. New amidine derivatives have been synthesized, among them N,N'-diphenyl-4-methoxy-benzamidine (methoxyamidine), which is effective against Leishmania amazonensis (LD50 = 20 μM) and Trypanosoma cruzi (LD50 = 59 nM)...
November 8, 2017: Chemico-biological Interactions
Hao-Bin Hu, Hai-Peng Liang, Hai-Ming Li, Ru-Nan Yuan, Jiao Sun, La-La Zhang, Ming-Hu Han, Yun Wu
Twenty natural stilbenoids (1-20), including seven new stilbenoids (2, 4-7, 19, 20) and thirteen known stilbenoids (1, 3, 8-18), were isolated from the stem barks of Acanthopanax leucorrhizus, and six modified stilbenoid derivatives (1a, 2a, 4a, 4b, 7a and 17a) were obtained via methylation, demethylation and isopentenylation of the corresponding isolates (1, 2, 4, 7 and 17). These stilbenoids were structurally characterized by comprehensive analysis of their spectroscopic data and comparison with literature information, and evaluated for their cytotoxic activities against three human tumor cell lines (leukemia HL-60, hepatoma SMMC-7721 and breast carcinoma MCF-7) in vitro by MTT assay...
November 8, 2017: Fitoterapia
Jun Okuda, Debabrata Mukherjee
Solid magnesium hydride [MgH2]∞ has been continuously pursued as a potential hydrogen storage material. Organic chemists were rather interested in soluble magnesium hydride reagents from mid-20th century. But it was only in the last two decades that molecular magnesium hydride chemistry received a major boost from organometallic chemists with a series of structurally well-characterized examples that continues to build a whole new class of compounds. Nearly 40 such species have been isolated, ranging from mononuclear terminal hydrides to large hydride clusters with more than 10 magnesium atoms...
November 11, 2017: Angewandte Chemie
Jae Sik Yu, Dahae Lee, Seoung Rak Lee, Jae Wook Lee, Chang-Ik Choi, Tae Su Jang, Ki Sung Kang, Ki Hyun Kim
The fruit of the white mulberry tree (Morus alba L.) is a multiple fruit with a sweet flavor commonly consumed around the world. Chemical investigation of the fruits led to the isolation of two indole acetic acid derivatives (1 -2) including a new compound, which turned out to be an isolation artifact, 3S-(β-D-glucopyranosyloxy)-2,3-dihydro-2-oxo-1H-indole-3-acetic acid butyl ester (1), along with five known compounds (3 -7). Compounds 2 and 7 were newly identified from mulberry fruit. The new isolation artifact (1) exhibited cytotoxic effect on human cervical cancer Hela cells in a dose-dependent manner...
November 7, 2017: Bioorganic Chemistry
Khalid A M Attia, Nasr M El-Abasawi, Ahmed El-Olemy, Ahmed Serag
In this article, one of the potential degradation products of the novel antiviral drug simeprevir was isolated and characterized by means of infrared (IR) and mass spectrometry. Moreover, comparative molecular docking, ADMET (absorption, distribution, metabolism, excretion - toxicity) and insilico toxicity prediction studies were applied to evaluate the activity of simeprevir and its degradation product. Furthermore,a simple, accurate and selective second derivative synchronous spectrofluorimetric method was developed for the determination of simeprevir in the presence of its oxidative degradation product...
November 10, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
Haiyang Xu, Gaurav D Moghe, Krystle Wiegert-Rininger, Anthony L Schilmiller, Cornelius S Barry, Robert L Last, Eran Pichersky
Flowers of Tanacetum cinerariifolium produce a set of compounds known collectively as pyrethrins, which are commercially important pesticides that are strongly toxic to flying insects but not to most vertebrates. A pyrethrin molecule is an ester consisting of either trans-chrysanthemic acid or its modified form, pyrethric acid, and one of three alcohols - jasmolone, pyrethrolone, and cinerolone - that appear to be derived from jasmonic acid. Chrysanthemyl diphosphate synthase (CDS), the first enzyme involved in the synthesis of trans-chrysanthemic acid, was previously characterized and its gene isolated...
November 9, 2017: Plant Physiology
Suman Sinha, Siuli Das, Rina Sikari, Seuli Parua, Paula Brandaõ, Serhiy Demeshko, Franc Meyer, Nanda D Paul
The new redox-noninnocent azoaromatic pincers 2-(arylazo)-1,10-phenanthroline (L(1)) and 2,9-bis(phenyldiazo)-1,10-phenanthroline (L(2)) are reported. The ligand L(1) is a tridentate pincer having NNN donor atoms, whereas L(2) is tetradentate having two azo-N donors and two N-donor atoms from the 1,10-phenanthroline moiety. Reaction of FeCl2 with L(1) or L(2) produced the pentacoordinated mixed-ligand Fe(II) complexes FeL(1)Cl2 (1) and FeL(2)Cl2 (2), respectively. Homoleptic octahedral Fe(II) complexes, mer-[Fe(L(1))2](ClO4)2 [3](ClO4)2 and mer-[Fe(L(2))2](ClO4)2 [4](ClO4)2, have been synthesized from the reaction of hydrated Fe(ClO4)2 and L(1) or L(2)...
November 9, 2017: Inorganic Chemistry
Li Luo, Wuhong Zheng, Guili Lian, Huaning Chen, Ling Li, Changsheng Xu, Liangdi Xie
The present study aimed to assess the effects of therapy with adiponectin (APN) gene-modified adipose-derived stem cells (ADSCs) on pulmonary arterial hypertension (PAH) in rats and the underlying cellular and molecular mechanisms. ADSCs were successfully isolated from the rats and characterized. ADSCs were effectively infected with the green fluorescent protein (GFP)-empty (ADSCs-V) or the APN-GFP (ADSCs-APN) lentivirus and the APN expression was evaluated by ELISA. Sprague-Dawley rats were administered monocrotaline (MCT) to develop PAH...
October 31, 2017: International Journal of Molecular Medicine
Petrina Kapewangolo, Martha Kandawa-Schulz, Debra Meyer
Ocimum plants are traditionally used to manage HIV/AIDS in various African countries. The effects of Ocimum labiatum extract on HIV-1 protease (PR) and reverse transcriptase (RT) is presented here along with characterization of an identified bioactive compound, achieved through ¹H- and (13)C-NMR. The extract's effect on HIV-1 replication was assessed by HIV-1 p24 antigen capture. Cytotoxicity of samples was evaluated using tetrazolium dyes and real-time cell electronic sensing (RT-CES). Ocimum labiatum inhibited HIV-1 PR with an IC50 value of 49...
November 6, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Anuj K Sharma, Jason W Schultz, John T Prior, Nigam P Rath, Liviu M Mirica
Positron emission tomography (PET) is emerging as one of the most important diagnostic tools for brain imaging, yet the most commonly used radioisotopes in PET imaging, (11)C and (18)F, have short half-lives, and their usage is thus somewhat limited. By comparison, the (64)Cu radionuclide has a half-life of 12.7 h, which is ideal for administering and imaging purposes. In spite of appreciable research efforts, high-affinity copper chelators suitable for brain imaging applications are still lacking. Herein, we present the synthesis and characterization of a series of bifunctional compounds (BFCs) based on macrocyclic 1,4,7-triazacyclononane and 2,11-diaza[3...
November 7, 2017: Inorganic Chemistry
Gerhard Erker, Xin Tao, Constantin G Daniliuc, Oliver Janka, Rainer Pöttgen, Robert Knitsch, Michael R Hansen, Hellmut Eckert, Maximilian Lübbesmeyer, Armido Studer, Gerald Kehr
Triplet dioxygen was reduced by TEMPO or trityl radicals in the presence of two molar equivalents of the strong B(p-C6F4X)3 (X: F or H) boron Lewis acids under mild conditions to give the bis(borane)superoxide systems 2. The sensitive radical anion species were isolated and characterized including X-ray crystal structure analysis and EPR spectroscopy.
November 7, 2017: Angewandte Chemie
Nadjet Rezki, Salsabeel A Al-Sodies, Sheikh Shreaz, Rayees Ahmad Shiekh, Mouslim Messali, Vaseem Raja, Mohamed R Aouad
A series of specific task ionic liquids (ILs) based on a pyridiniumhydrazone scaffold in combination with hexafluorophosphate (PF₆(-)), tetrafluoroboron (BF₄(-)) and/or trifluoroacetate (CF₃COO(-)) counter anion, were designed and characterized by IR, NMR and mass spectrometry. The reactions were conducted under both conventional and green ultrasound procedures. The antifungal potential of the synthesized compounds 2-25 was investigated against 40 strains of Candida (four standard and 36 clinical isolates)...
November 7, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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