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Isolation and characterization of compounds

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https://www.readbyqxmd.com/read/28538206/spectroscopic-far-or-terahertz-mid-infrared-and-raman-investigation-thermal-analysis-and-biological-activity-of-piplartine
#1
Anubha Srivastava, T Karthick, B D Joshi, Rashmi Mishra, Poonam Tandon, A P Ayala, Javier Ellena
Research in the field of medicinal plants including Piper species like long pepper (Piper longum L.- Piperaceae) is increasing all over the world due to its use in traditional and Ayurvedic medicine. Piplartine (piperlongumine, 5,6-dihydro-1-[(2E)-1-oxo-3-(3,4,5-trimethoxyphenyl)-2-propenyl]-2(1H)-pyridinone), a biologically active alkaloid/amide was isolated from the phytochemical investigations of Piper species, as long pepper. This alkaloid has cytotoxic, anti-fungal, anti-diabetic, anti-platelet aggregation, anti-tumoral, anxiolytic, anti-depressant, anti-leishmanial, and genotoxic activities, but, its anticancer property is the most promising and has been widely explored...
May 5, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28537710/elucidating-adsorptive-fractions-of-natural-organic-matter-on-carbon-nanotubes
#2
Mohamed Ateia, Onur G Apul, Yuta Shimizu, Astri Muflihah, Chihiro Yoshimura, Tanju Karanfil
Natural organic matter (NOM) is a heterogeneous mixture of organic compounds that is omnipresent in natural waters. To date, our understanding of NOM components' adsorption by carbon nanotubes (CNTs) is limited due to limited number of comprehensive studies in the literature examining the adsorption of NOM by CNTs. In this study, eleven standard NOMs from various sources were characterized, and their adsorption on four different CNTs were examined side-by-side using total organic carbon, fluorescence, UV-visible spectroscopy, and high performance size exclusion chromatography (HPSEC) analysis...
May 24, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28535606/isolation-purification-and-characterization-of-five-active-diketopiperazine-derivatives-from-endophytic-streptomyces-suk-25-with-antimicrobial-and-cytotoxic-activities
#3
Muhanna M Alshaibani, Juriyati Jalil, Nik M Sidik, Siti Junaidah Ahmad, Nurkhalida Kamal, Ruangelie Edrada-Ebel, Noraziah M Zin
On our search for new sources of bioactive secondary metabolites from Streptomyces sp., the ethyl acetate extracts from the endophytic Streptomyces SUK 25 afforded five active diketopiperazines compounds. The aim of this study was to characterize the bioactive compounds isolated from endophytic Streptomyces SUK 25 and evaluate its bioactivity against multiple drug resistance (MDR) bacteria such as Enterococcus raffinosus, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa and Enterobacter spp...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28535483/cloning-and-functional-characterization-of-a-retinoid-x-receptor-orthologue-in-platynereis-dumerilii-an-evolutionary-and-toxicological-perspective
#4
Ana André, Raquel Ruivo, Ana Capitão, Elsa Froufe, Inês Páscoa, Luís Filipe Costa Castro, Miguel Machado Santos
In the present work we provide the first isolation and functional characterization of a RXR orthologue in an annelid species, the Platynereis dumerilii. Using an in vitro luciferase reporter assay we evaluated the annelid receptor ability to respond to ligand 9-cis-retinoic acid, TBT and TPT. Our results show that the annelid RXR responds to 9-cis-retinoic acid and to the organotins by activating reporter gene transcription. The findings suggest a conserved mode of action of the receptor in response to a common signaling molecule and modulation by organotin compounds between vertebrates and Lophotrochozoans...
May 20, 2017: Chemosphere
https://www.readbyqxmd.com/read/28535423/chromatographic-separation-and-detection-methods-of-aloe-arborescens-miller-constituents-a-systematic-review
#5
REVIEW
Jilan A Nazeam, Haidy A Gad, Hala M El-Hefnawy, Abdel-Naser B Singab
Aloe arborescens Miller (Family Asphodelaceae) is a member of genus Aloe, which is used in traditional medicine to cure various diseases. The extracts of the plant have been reported to possess anticancer, immunomodulator, antidiabetic, anti-inflammatory and antioxidant activities. The phytochemical investigations have revealed diverse chemical constituents, including phenolics [anthraquinones, anthrones, pyrones, chromones and coumarins], polysaccharides [arborans [(1-4) linked glucomannans, polysaccharide (A, B and C): (A: a linear (1-6)-O-α-glucan, B: a branching (1-2)-O-l-arabinose with (1-2)-O-d-galactose linkages and C: (1-4)-O-β-mannan with 18% acetyl group)]], glycoproteins and carboxypeptidase enzyme...
April 29, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28534864/study-on-the-alkylation-reactions-of-n-7-unsubstituted-1-3-diazaoxindoles
#6
Eszter Kókai, Judit Halász, András Dancsó, József Nagy, Gyula Simig, Balázs Volk
The chemistry of the 5,7-dihydro-6H-pyrrolo[2,3-d]pyrimidin-6-one (1,3-diazaoxindole) compound family, possessing a drug-like scaffold, is unexplored. In this study, the alkylation reactions of N(7)-unsubstituted 5-isopropyl-1,3-diazaoxindoles bearing various substituents at the C(2) position have been investigated. The starting compounds were synthesized from the C(5)-unsubstituted parent compounds by condensation with acetone and subsequent catalytic reduction of the 5-isopropylidene moiety. Alkylation of the thus obtained 5-isopropyl derivatives with methyl iodide or benzyl bromide in the presence of a large excess of sodium hydroxide led to 5,7-disubstituted derivatives...
May 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28533069/enrichment-and-characterization-of-hydrocarbon-degrading-bacteria-from-petroleum-refinery-waste-as-potent-bioaugmentation-agent-for-in-situ-bioremediation
#7
Poulomi Sarkar, Ajoy Roy, Siddhartha Pal, Balaram Mohapatra, Sufia K Kazy, Mrinal K Maiti, Pinaki Sar
Intrinsic biodegradation potential of bacteria from petroleum refinery waste was investigated through isolation of cultivable strains and their characterization. Pseudomonas and Bacillus spp. populated the normal cultivable taxa while prolonged enrichment with hydrocarbons and crude oil yielded hydrocarbonoclastic bacteria of genera Burkholderia, Enterobacter, Kocuria, Pandoraea, etc. Strains isolated through enrichment showed assemblages of superior metabolic properties: utilization of aliphatic (C6-C22) and polyaromatic compounds, anaerobic growth with multiple terminal electron acceptors and higher biosurfactant production...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28525713/identification-and-validation-of-sensory-active-compounds-from-data-driven-research-a-flavoromics-approach
#8
Ian Ronningen, Michelle Miller, Youlin Xia, Devin G Peterson
In the current study significant LC/MS features, from multivariate analysis (MVA) models developed using untargeted chemical fingerprinting to characterize flavor changes in extracts of citrus fruits related to aging, were further isolated and analyzed for sensory relevant information, followed by structural elucidation of flavor actives. The top ten statistical features from two MVA approaches, partial least squares data analysis (PLS-DA) and Random Forrest (RF), were purified to approximately 70% via multi-dimensional liquid chromatography-mass directed fractionation to screen for sensory activity...
May 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28521385/-ziziphus-oxyphylla-ethnobotanical-ethnopharmacological-and-phytochemical-review
#9
REVIEW
Rizwan Ahmad, Niyaz Ahmad, Atta Abbas Naqvi
ETHNOPHARMACOLOGICAL RELEVANCE: Ziziphus oxyphylla (ZO) is distributed mainly in tropic and warm temperate regions in the world. Pakistan owns six (06) indigenous species of genus Ziziphus out of which ZO is widely used for traditional treatment of different ailments such as diabetes, jaundice and liver diseases. AIM OF THE STUDY: The present review aims to provide in-depth and comprehensive literature overview, regarding botanical, chemical and biological characteristics of the plant alongwith phytochemical isolation and mechanistic studies to support its folklore and traditional uses...
May 14, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28521273/rapid-discovery-of-cyclopamine-analogs-from-fritillaria-and-veratrum-plants-using-lc-q-tof-ms-and-lc-qqq-ms
#10
Yuan Du, Zu-Guo Zheng, Yue Yu, Zi-Tian Wu, Dan Liang, Ping Li, Yan Jiang, Hui-Jun Li
Cyclopamine, an inhibitor of the Hedgehog (Hh) signaling pathway, has been paid much attention in treating a wide variety of tumors. However, isolation and purification of cyclopamine analogs from medicinal plants remain challengeable. We herein proposed an efficient strategy using liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS) and liquid chromatography triple-quadrupole mass spectrometry (LC-QqQ-MS) for rapid screening and targeted isolation of cyclopamine analogs in Fritillaria and Veratrum plants...
May 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28514101/rearrangement-of-a-p4-butterfly-complex-the-formation-of-a-homoleptic-phosphorus-iron-sandwich-complex
#11
Julian Müller, Sebastian Heinl, Christoph Schwarzmaier, Gábor Balázs, Martin Keilwerth, Karsten Meyer, Manfred Scheer
The versatile coordination behavior of the P4 butterfly complex [{Cp'''Fe(CO)2 }2 (μ,η(1:1) -P4 )] (1, Cp'''=η(5) -C5 H2(t) Bu3 ) towards different iron(II) compounds is presented. The reaction of 1 with [FeBr2 ⋅dme] (dme=dimethoxyethane) leads to the chelate complex [{Cp'''Fe(CO)2 }2 (μ3 ,η(1:1:2) -P4 ){FeBr2 }] (2), whereas, in the reaction with [Fe(CH3 CN)6 ][PF6 ]2 , an unprecedented rearrangement of the P4 butterfly structural motif leads to the cyclo-P4 moiety in {(Cp'''Fe(CO)2 )2 (μ3 ,η(1:1:4) -P4 )}2 Fe][PF6 ]2 (3)...
May 17, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28513553/antibacterial-activity-of-7-epiclusianone-and-its-novel-copper-metal-complex-on-streptococcus-spp-isolated-from-bovine-mastitis-and-their-cytotoxicity-in-mac-t-cells
#12
Mariana de Barros, Pedro Griffo Perciano, Marcelo Henrique Dos Santos, Leandro Licursi De Oliveira, Éderson D'Martin Costa, Maria Aparecida Scatamburlo Moreira
Mastitis is an inflammation of mammary gland parenchyma that adversely affects bovine health and dairy production worldwide despite significant efforts to eradicate it. The aim of this work was to characterize the antimicrobial activity of 7-epiclusianone (7-epi), a compound extracted from the Rheedia brasiliensis fruit, its complex with copper against Streptococcus spp. isolated from bovine mastitis, and to assess their cytotoxicity to bovine mammary alveolar cells (MAC-T). The complex 7-epiclusianone-Cu (7-epi-Cu) was an amorphous green solid with optical activity...
May 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28511892/occurrence-and-biosynthesis-of-carotenoids-in-phytoplankton
#13
REVIEW
Jim Junhui Huang, Shaoling Lin, Wenwen Xu, Peter Chi Keung Cheung
Naturally occurring carotenoids are important sources of antioxidants, anti-cancer compounds and anti-inflammatory agents and there is thus considerable market demand for their pharmaceutical applications. Carotenoids are widely distributed in marine and freshwater organisms including microalgae, phytoplankton, crustaceans and fish, as well as in terrestrial plants and birds. Recently, phytoplankton-derived carotenoids have received much attention due to their abundance, rapid rate of biosynthesis and unique composition...
May 13, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28508699/antimicrobial-anticancer-and-antioxidant-compounds-from-premna-resinosa-growing-in-saudi-arabia
#14
Dina A Albadawi, Ramzi A Mothana, Jamal M Khaled, Abdelkhader E Ashour, Ashok Kumar, Sheikh F Ahmad, Mansour S Al-Said, Adnan J Al-Rehaily, Nawal M Almusayeib
CONTEXT: Premna resinosa (Hochst.) Schauer (Lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage. OBJECTIVE: This study evaluates the anticancer, antimicrobial and antioxidant activities of P. resinosa, and isolates some responsible constituents. MATERIALS AND METHODS: The methanol extract of P. resinosa aerial parts and its fractions (n-hexane, dichloromethane, ethyl acetate and n-butanol) were tested...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28507481/isolation-and-characterization-of-phthalates-from-brevibacterium-mcbrellneri-that-cause-cytotoxicity-and-cell-cycle-arrest
#15
Maheshwari Rajamanikyam, Varahalarao Vadlapudi, Sai Prathima Parvathaneni, Dhevendar Koude, Prabhakar Sripadi, Sunil Misra, Ramars Amanchy, Suryanarayana Murty Upadhyayula
Bacteria belonging to the family Brevibacterieae are ubiquitous Gram positive organisms that are responsible for the feet odour and cheese aroma. Brevibacterium mcbrellneri is a relatively new member belonging to Brevibacterieae. In the current manuscript we discuss isolation of biologically active metabolites from Brevibacterium mcbrellneri. Two aromatic esters were isolated from Brevibacterium mcbrellneri by "Bioassay guided fractionation strategy" and identified as di-(2-ethylhexyl) phthalate and dibutyl phthalate by chemical characterization using biophysical techniques...
2017: EXCLI journal
https://www.readbyqxmd.com/read/28506901/new-isoflavonoids-from-the-extract-of-rhynchosia-precatoria-humb-bonpl-ex-willd-dc-and-their-antimycobacterial-activity
#16
Enrique Wenceslao Coronado-Aceves, Giulia Gigliarelli, Adriana Garibay-Escobar, Ramón Enrique Robles Zepeda, Massimo Curini, Jaime López Cervantes, Clara Inés Espitia-Pinzón, Stefano Superchi, Stefania Vergura, Maria Carla Marcotullio
ETHNOPHARMACOLOGY RELEVANCE: The evaluation of the antimycobacterial activity of extracts of medicinal plants used by Mayos against tuberculosis and respiratory problems, allowed the identification of Rhynchosia precatoria (Humb. & Bonpl. ex Willd.) DC (Fabaceae) as the best candidate to find new antimycobacterial compounds. AIM OF THE STUDY: To isolate and characterize the compounds of R. precatoria responsible for the inhibitory and bactericidal activity against Mycobacterium tuberculosis H37Rv and Mycobacterium smegmatis ATCC 700084...
May 12, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28506279/uncovering-the-trimethylamine-producing-bacteria-of-the-human-gut-microbiota
#17
Silke Rath, Benjamin Heidrich, Dietmar H Pieper, Marius Vital
BACKGROUND: Trimethylamine (TMA), produced by the gut microbiota from dietary quaternary amines (mainly choline and carnitine), is associated with atherosclerosis and severe cardiovascular disease. Currently, little information on the composition of TMA producers in the gut is available due to their low abundance and the requirement of specific functional-based detection methods as many taxa show disparate abilities to produce that compound. RESULTS: In order to examine the TMA-forming potential of microbial communities, we established databases for the key genes of the main TMA-synthesis pathways, encoding choline TMA-lyase (cutC) and carnitine oxygenase (cntA), using a multi-level screening approach on 67,134 genomes revealing 1107 and 6738 candidates to exhibit cutC and cntA, respectively...
May 15, 2017: Microbiome
https://www.readbyqxmd.com/read/28502905/inhibition-of-proinflammatory-mediators-by-coumaroyl-lupendioic-acid-a-new-lupane-type-triterpene-from-careya-arborea-on-inflammation-induced-animal-model
#18
Rayhana Begum, Manjur Ali Sheliya, Showkat R Mir, Ekta Singh, Manju Sharma
ETHNOPHARMACOLOGICAL RELEVANCE: Careya arborea Roxb. (Lecythidaceae) is a large tree found throughout India in deciduous forests and grasslands. C. arborea is traditionally used in tumors, inflammation, anthelmintic, bronchitis, epileptic fits, astringents, antidote to snake-venom, skin disease, diarrhea, dysentery with bloody stools, dyspepsia, ulcer, tooth ache, and ear pain. AIM OF THE STUDY: In our previous work, the methanolic extract of Careya arborea stem bark showed significant anti-inflammatory activity...
May 11, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28501748/systematic-screening-and-characterization-of-prototype-constituents-and-metabolites-of-total-astragalosides-using-hplc-esi-it-tof-ms-n-after-oral-administration-to-rats
#19
Hong-Fu Li, Feng Xu, Ping Yang, Guang-Xue Liu, Ming-Ying Shang, Xuan Wang, Jun Yin, Shao-Qing Cai
Astragalosides (AGs) are the main bioactive constituents in Astragali Radix (AR), and have a wide range of pharmacological properties, including immunoregulatory, cardioprotective, neuroprotective, antioxidative, antidiabetic, and antinociceptive effects. However, the metabolism of total AGs remains unclear. To clarify the metabolic fate of AGs after oral administration to rats, total AGs were isolated from AR extracts using AB-8 macroporous resin chromatography and preparative HPLC, and then analyzed using HPLC-DAD-ELSD and LC-MS...
May 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28500722/uv-resistant-actinobacteria-from-high-altitude-andean-lakes-isolation-characterization-and-antagonistic-activities
#20
María Cecilia Rasuk, Gabriela Mónica Ferrer, Daniel Kurth, Luciano Raúl Portero, María Eugenia Farías, Virginia Helena Albarracín
Polyextremophiles are present in a wide variety of extreme environments in which they must overcome various hostile conditions simultaneously such as high UVB radiation, extreme pHs and temperatures, elevated salt and heavy-metal concentration, low-oxygen pressure and scarce nutrients. High-altitude Andean lakes (HAALs; between 2000 and 4000 m) are one example of these kinds of ecosystems suffering from the highest total solar and UVB radiation on Earth where an abundant and diverse polyextremophilic microbiota was reported...
May 2017: Photochemistry and Photobiology
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