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Preadipocyte hypoxia

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https://www.readbyqxmd.com/read/27625023/hif-1%C3%AE-in-myeloid-cells-promotes-adipose-tissue-remodeling-toward-insulin-resistance
#1
Akiko Takikawa, Arshad Mahmood, Allah Nawaz, Tomonobu Kado, Keisuke Okabe, Seiji Yamamoto, Aminuddin Aminuddin, Satoko Senda, Koichi Tsuneyama, Masashi Ikutani, Yasuharu Watanabe, Yoshiko Igarashi, Yoshinori Nagai, Kiyoshi Takatsu, Keiichi Koizumi, Johji Imura, Nobuhito Goda, Masakiyo Sasahara, Michihiro Matsumoto, Kumiko Saeki, Takashi Nakagawa, Shiho Fujisaka, Isao Usui, Kazuyuki Tobe
Adipose tissue hypoxia is an important feature of pathological adipose tissue expansion. Hypoxia-inducible factor-1α (HIF-1α) in adipocytes reportedly induces oxidative stress and fibrosis, rather than neoangiogenesis via vascular endothelial growth factor (VEGF)-A. We previously reported that macrophages in crown-like structures (CLSs) are both hypoxic and inflammatory. In the current study, we examined how macrophage HIF-1α is involved in high-fat diet (HFD)-induced inflammation, neovascularization, hypoxia, and insulin resistance using mice with myeloid cell-specific HIF-1α deletion that were fed an HFD...
December 2016: Diabetes
https://www.readbyqxmd.com/read/27421877/effects-of-acute-hypoxia-on-human-adipose-tissue-lipoprotein-lipase-activity-and-lipolysis
#2
Bimit Mahat, Étienne Chassé, Jean-François Mauger, Pascal Imbeault
BACKGROUND: Adipose tissue regulates postprandial lipid metabolism by storing dietary fat through lipoprotein lipase-mediated hydrolysis of exogenous triglycerides, and by inhibiting delivery of endogenous non-esterified fatty acid to nonadipose tissues. Animal studies show that acute hypoxia, a model of obstructive sleep apnea, reduces adipose tissue lipoprotein lipase activity and increases non-esterified fatty acid release, adversely affecting postprandial lipemia. These observations remain to be tested in humans...
2016: Journal of Translational Medicine
https://www.readbyqxmd.com/read/27374117/vitamin-d-controls-resistance-artery-function-through-regulation-of-perivascular-adipose-tissue-hypoxia-and-inflammation
#3
Christopher J Pelham, Elizabeth M Drews, Devendra K Agrawal
Vitamin D deficiency in human subjects is associated with hypertension, metabolic syndrome and related risk factors of cardiovascular diseases. Serum 25-hydroxyvitamin D levels correlate inversely with adiposity in obese and lean individuals. Bioactive vitamin D, or calcitriol, exerts anti-inflammatory effects on adipocytes, preadipocytes and macrophages in vitro. We tested the hypothesis that vitamin D deficiency alters the phenotype of perivascular adipose tissue (PVAT) leading to impaired function in resistance artery...
September 2016: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/27246913/nitric-oxide-produced-by-macrophages-inhibits-adipocyte-differentiation-and-promotes-profibrogenic-responses-in-preadipocytes-to-induce-adipose-tissue-fibrosis
#4
Jung Eun Jang, Myoung Seok Ko, Ji-Young Yun, Mi-Ok Kim, Jin Hee Kim, Hye Sun Park, Ah-Ram Kim, Hyuk-Joong Kim, Bum Joong Kim, Young Eun Ahn, Jin Sun Oh, Woo Je Lee, Robert A Harris, Eun Hee Koh, Ki-Up Lee
Fibrosis of adipose tissue induces ectopic fat accumulation and insulin resistance by inhibiting adipose tissue expandability. Mechanisms responsible for the induction of adipose tissue fibrosis may provide therapeutic targets but are poorly understood. In this study, high-fat diet (HFD)-fed wild-type (WT) and iNOS(-/-) mice were used to examine the relationship between nitric oxide (NO) produced by macrophages and adipose tissue fibrosis. In contrast to WT mice, iNOS(-/-) mice fed an HFD were protected from infiltration of proinflammatory macrophages and adipose tissue fibrosis...
September 2016: Diabetes
https://www.readbyqxmd.com/read/27023342/the-effect-of-pericellular-oxygen-levels-on-proteomic-profile-and-lipogenesis-in-3t3-l1-differentiated-preadipocytes-cultured-on-gas-permeable-cultureware
#5
Martin Weiszenstein, Nela Pavlikova, Moustafa Elkalaf, Petr Halada, Ondrej Seda, Jan Trnka, Jan Kovar, Jan Polak
Pericellular oxygen concentration represents an important factor in the regulation of cell functions, including cell differentiation, growth and mitochondrial energy metabolism. Hypoxia in adipose tissue has been associated with altered adipokine secretion profile and suggested as a possible factor in the development of type 2 diabetes. In vitro experiments provide an indispensable tool in metabolic research, however, physical laws of gas diffusion make prolonged exposure of adherent cells to desired pericellular O2 concentrations questionable...
2016: PloS One
https://www.readbyqxmd.com/read/26885267/adipocytes-exert-lipotoxic-effects-on-osteoblast-through-activating-hypoxia-signaling-pathway-in-vitro
#6
Qi Zhu, Lin Wang, Yue Zuo, Chang Shan, Jing Yu, Lige Song, Keqin Zhang
This study aimed to investigate the effect of adipocytes on osteoblastic bone formation in vitro. The differentiated and undifferentiated 3T3-L1 cells were co-cultured with primary calvarial osteoblasts. At 48 h, proliferated osteoblasts decreased significantly after co-culture with differentiated preadipocytes as compared to those co-cultured with undifferentiated preadipocytes; at 7 days, the expressions of bone formation-related genes decreased in osteoblasts co-cultured with differentiated preadipocytes; at 14 days, osteoblasts mineralization also decreased significantly after co-culture with differentiated preadipocytes...
2015: American Journal of Translational Research
https://www.readbyqxmd.com/read/26881257/hypoxia-alters-the-expression-of-dipeptidyl-peptidase-4-and-induces-developmental-remodeling-of-human-preadipocytes
#7
Helena H Chowdhury, Jelena Velebit, Nataša Radić, Vito Frančič, Marko Kreft, Robert Zorec
Dipeptidyl peptidase 4 (DPP4), a transmembrane protein, has been identified in human adipose tissue and is considered to be associated with obesity-related type 2 diabetes. Since adipose tissue is relatively hypoxic in obese participants, we investigated the expression of DPP4 in human preadipocytes (hPA) and adipocytes in hypoxia, during differentiation and upon insulin stimulation. The results show that DPP4 is abundantly expressed in hPA but very sparsely in adipocytes. During differentiation in vitro, the expression of DPP4 in hPA is reduced on the addition of differentiation medium, indicating that this protein can be hPA marker...
2016: Journal of Diabetes Research
https://www.readbyqxmd.com/read/26219415/transient-hypoxia-reprograms-differentiating-adipocytes-for-enhanced-insulin-sensitivity-and-triglyceride-accumulation
#8
H Lu, Z Gao, Z Zhao, J Weng, J Ye
OBJECTIVES: To investigate the impact of transient (2-4 h) hypoxia on metabolic reprogramming of adipocytes. METHODS: The impact of transient hypoxia on metabolic reprogramming was investigated in 3T3-L1 cells before and after differentiation. Glucose uptake, fatty acid oxidation, lipolysis and mitochondria were examined to determine the hypoxia effects. Preadipocytes were exposed to transient hypoxia (4 h day(-1)) in the course of differentiation. Insulin sensitivity and triglyceride (TG) accumulation was examined in the cells at the end of differentiation to determine the reprogramming effects...
January 2016: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/25987952/adipose-tissue-fibrosis
#9
Christa Buechler, Sabrina Krautbauer, Kristina Eisinger
The increasing prevalence of obesity causes a major interest in white adipose tissue biology. Adipose tissue cells are surrounded by extracellular matrix proteins whose composition and remodeling is of crucial importance for cell function. The expansion of adipose tissue in obesity is linked to an inappropriate supply with oxygen and hypoxia development. Subsequent activation of hypoxia inducible factor 1 (HIF-1) inhibits preadipocyte differentiation and initiates adipose tissue fibrosis. Thereby adipose tissue growth is limited and excess triglycerides are stored in ectopic tissues...
May 15, 2015: World Journal of Diabetes
https://www.readbyqxmd.com/read/25857555/tnf-related-apoptosis-inducing-ligand-promotes-human-preadipocyte-proliferation-via-erk1-2-activation
#10
Jan-Bernd Funcke, Verena Zoller, Muad Abd El Hay, Klaus-Michael Debatin, Martin Wabitsch, Pamela Fischer-Posovszky
Upon obesity, adipose tissue is excessively expanded and characterized by pathologic processes like hypoxia, fibrosis, and inflammation. Death ligands belonging to the TNF superfamily such as TNF-α are important contributors to these derangements and exert a pronounced influence on the metabolic and cellular homeostasis of adipose tissue. Here, we sought to identify the effect of the death ligand TNF-related apoptosis-inducing ligand (TRAIL) on the adipose tissue precursor cell pool and therefore investigated its influence on preadipocyte proliferation...
July 2015: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/25054244/normobaric-hyperoxygenation-enhances-initial-survival-regeneration-and-final-retention-in-fat-grafting
#11
Harunosuke Kato, Jun Araki, Kentaro Doi, Shinichiro Kuno, Kahori Kinoshita, Kazuhide Mineda, Koji Kanayama, Kotaro Yoshimura
BACKGROUND: Fat grafting is a promising modality for soft-tissue augmentation/reconstruction. However, grafted fat tissue is not initially perfused and relies on plasmatic diffusion from the recipient bed until revascularization occurs. The authors evaluated the therapeutic effects of normobaric hyperoxygenation for enhancing fat graft retention. METHODS: Aspirated human fat tissue was cultured under tissue hypoxia (1% oxygen), normoxia (6%), and hyperoxia (20%) levels, and evaluated for adipocyte viability...
November 2014: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/24819450/hypoxia-and-adipocyte-physiology-implications-for-adipose-tissue-dysfunction-in-obesity
#12
REVIEW
Paul Trayhurn
Hypoxia develops in white adipose tissue in obese mice, resulting in changes in adipocyte function that may underpin the dysregulation that leads to obesity-associated disorders. Whether hypoxia occurs in adipose tissue in human obesity is unclear, with recent studies contradicting earlier reports that this was the case. Adipocytes, both murine and human, exhibit extensive functional changes in culture in response to hypoxia, which alters the expression of up to 1,300 genes. These include genes encoding key adipokines such as leptin, interleukin (IL)-6, vascular endothelial growth factor (VEGF), and matrix metalloproteinase-2 (MMP-2), which are upregulated, and adiponectin, which is downregulated...
2014: Annual Review of Nutrition
https://www.readbyqxmd.com/read/24513287/lipid-synthesis-is-promoted-by-hypoxic-adipocyte-derived-exosomes-in-3t3-l1-cells
#13
Soichi Sano, Yasukatsu Izumi, Takehiro Yamaguchi, Takanori Yamazaki, Masako Tanaka, Masayuki Shiota, Mayuko Osada-Oka, Yasuhiro Nakamura, Min Wei, Hideki Wanibuchi, Hiroshi Iwao, Minoru Yoshiyama
Hypoxia occurs within adipose tissues as a result of adipocyte hypertrophy and is associated with adipocyte dysfunction in obesity. Here, we examined whether hypoxia affects the characteristics of adipocyte-derived exosomes. Exosomes are nanovesicles secreted from most cell types as an information carrier between donor and recipient cells, containing a variety of proteins as well as genetic materials. Cultured differentiated 3T3-L1 adipocytes were exposed to hypoxic conditions and the protein content of the exosomes produced from these cells was compared by quantitative proteomic analysis...
March 7, 2014: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/24482687/hypoxia-induced-changes-of-sepp1-expression-in-rat-preadipocytes-and-its-impact-on-vascular-fibroblasts
#14
Lingni Yin, Wenwei Cai, Jing Sheng, Yun Sun
Human adipose tissues secret a lot of cytokines involved in physiological and pathological activities. Inflammation around blood vessels is positively related to the severity of atherosclerosis. This study was to investigate the impact of adipokine SePP1 on vascular fibroblasts (VF) under a hypoxia condition might provide new evidence and methods for treatment of atherosclerosis. The mRNA and protein expression of IL-6, MCP-1 and SePP1 were detected in preadipocytes under normoxic (21% O2) and hyperoxic (4% O2) conditions, and the impact of IL-6, MCP-1 and SePP1 on VF was investigated...
2014: International Journal of Clinical and Experimental Medicine
https://www.readbyqxmd.com/read/24302003/hepatocyte-growth-factor-regulates-neovascularization-in-developing-fat-pads
#15
Heather M White, Anthony J Acton, Malgorzata M Kamocka, Robert V Considine
In this study, we used lentiviral-delivered shRNA to generate a clonal line of 3T3-F442A preadipocytes with stable silencing of hepatocyte growth factor (HGF) expression and examined the long-term consequence of this modification on fat pad development. HGF mRNA expression was reduced 94%, and HGF secretion 79% (P < 0.01), compared with preadipocytes treated with nontargeting shRNA. Fat pads derived from HGF knockdown preadipocytes were significantly smaller (P < 0.01) than control pads beginning at 3 days postinjection (0...
January 15, 2014: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/24140569/hypoxia-mimetic-effects-in-the-secretome-of-human-preadipocytes-and-adipocytes
#16
Anja Rosenow, Jean-Paul Noben, Freek G Bouwman, Edwin C M Mariman, Johan Renes
White adipose tissue (WAT) regulates energy metabolism by secretion of proteins with endocrine and paracrine effects. Dysregulation of the secretome of obesity-associated enlarged WAT may lead to obesity-related disorders. This can be caused by hypoxia as a result of poorly vascularized WAT. The effect of hypoxia on the secretome of human (pre)adipocytes is largely unknown. Therefore, we investigated the effect of CoCl2, a hypoxia mimetic, on the secretome of human SGBS (pre)adipocytes by a proteomics approach combined with bioinformatic analysis...
December 2013: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/23900840/hypoxia-inducible-factor-2%C3%AE-dependent-hypoxic-induction-of-wnt10b-expression-in-adipogenic-cells
#17
Young-Kwon Park, Bongju Park, Seongyeol Lee, Kang Choi, Yunwon Moon, Hyunsung Park
Adipocyte hyperplasia and hypertrophy in obesity can lead to many changes in adipose tissue, such as hypoxia, metabolic dysregulation, and enhanced secretion of cytokines. In this study, hypoxia increased the expression of Wnt10b in both human and mouse adipogenic cells, but not in hypoxia-inducible factor (HIF)-2α-deficient adipogenic cells. Chromatin immunoprecipitation analysis revealed that HIF-2α, but not HIF-1α, bound to the Wnt10b enhancer region as well as upstream of the Wnt1 gene, which is encoded by an antisense strand of the Wnt10b gene...
September 6, 2013: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/23750014/fxr-activation-normalizes-insulin-sensitivity-in-visceral-preadipocytes-of-a-rabbit-model-of-mets
#18
Elena Maneschi, Linda Vignozzi, Annamaria Morelli, Tommaso Mello, Sandra Filippi, Ilaria Cellai, Paolo Comeglio, Erica Sarchielli, Alessandra Calcagno, Benedetta Mazzanti, Roberto Vettor, Gabriella Barbara Vannelli, Luciano Adorini, Mario Maggi
Insulin resistance is the putative key underlying mechanism linking adipose tissue (AT) dysfunction with liver inflammation and steatosis in metabolic syndrome (MetS). We have recently demonstrated that the selective farnesoid X receptor (FXR) agonist obeticholic acid (OCA) ameliorates insulin resistance and the metabolic profile with a marked reduction in the amount of visceral AT (VAT) in a high-fat diet (HFD)-induced rabbit model of MetS. These effects were mediated by the activation of FXR, since treatment with the selective TGR5 agonist INT-777 was not able to ameliorate the metabolic parameters evaluated...
2013: Journal of Endocrinology
https://www.readbyqxmd.com/read/23626778/insulin-regulates-hypoxia-inducible-factor-1%C3%AE-transcription-by-reactive-oxygen-species-sensitive-activation-of-sp1-in-3t3-l1-preadipocyte
#19
Sudipta Biswas, Reshmi Mukherjee, Nisha Tapryal, Amit K Singh, Chinmay K Mukhopadhyay
Oxygen sensing transcription factor HIF-1 is activated due to accumulation of regulatory subunit HIF-1α by posttranslational stability mechanism during hypoxia or by several other stimuli even in normoxia. HIF-1α is also regulated by NF-kB mediated transcription mechanism. Reactive oxygen species (ROS) act as an important regulator of HIF-1 either by affecting prolyl hydroxylase activity, the critical determinant of HIF-1α stabilization or by activating NF-kB to promote HIF-1α transcription. Insulin is known to activate HIF-1 by a ROS dependent mechanism but the molecular mechanism of HIF-1α regulation is not known so far...
2013: PloS One
https://www.readbyqxmd.com/read/23542713/activation-of-a-retinoic-acid-receptor-pathway-by-thiazolidinediones-induces-production-of-vascular-endothelial-growth-factor-vascular-permeability-factor-in-op9-adipocytes
#20
Daisuke Kotake, Noriyasu Hirasawa
Thiazolidinediones, ligands of peroxisome proliferator-activated receptorγ (PPARγ), are used in the management of type 2 diabetes mellitus. However, they can cause edema, which often leads to a discontinuation of treatment. The mechanism by which thiazolidinediones induce edema is poorly understood. We have confirmed that troglitazone (TGZ), a thiazolidinedione, induced the differentiation of a preadipocyte cell line, OP9, into adipocytes. The differentiated OP9 cells produced vascular permeability factors and the activity was completely neutralized by an antibody against vascular endothelial growth factor (VEGF)...
May 5, 2013: European Journal of Pharmacology
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