keyword
MENU ▼
Read by QxMD icon Read
search

circulation tumor cell

keyword
https://www.readbyqxmd.com/read/29474983/an-in-depth-evaluation-of-the-validity-and-logistics-surrounding-the-testing-of-ar-v7-mrna-expression-in-circulating-tumor-cells
#1
Anieta M Sieuwerts, Bianca Mostert, Michelle van der Vlugt-Daane, Jaco Kraan, Corine Beaufort, Mai Van, Wendy J C Prager, Bram De Laere, Nick Beije, Paul Hamberg, Hans M Westgeest, Metin Tascilar, Luc Y Dirix, Wendy Onstenk, Ronald de Wit, Martijn P Lolkema, Ron H J Mathijssen, John W M Martens, Stefan Sleijfer
Recent reports have emphasized the clinical relevance of detecting the androgen receptor splice variant 7 (AR-V7) in circulating tumor cells (CTCs). Our aim was to set up a validated multicenter pipeline to measure AR-V7 by RT-qPCR in RNA isolated from CellSearch-enriched CTCs to provide an AR-V7 positive/negative score in a clinically acceptable time range. CellSearch-enirched CTCs from metastatic castration-resistant prostate cancer patients were characterized by RT-qPCR. After optimization it was prospectively tested whether it was possible to report the AR-V7 status within 11 days (PRELUDE study)...
February 20, 2018: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/29474906/a-hematogenous-route-for-medulloblastoma-leptomeningeal-metastases
#2
Livia Garzia, Noriyuki Kijima, A Sorana Morrissy, Pasqualino De Antonellis, Ana Guerreiro-Stucklin, Borja L Holgado, Xiaochong Wu, Xin Wang, Michael Parsons, Kory Zayne, Alex Manno, Claudia Kuzan-Fischer, Carolina Nor, Laura K Donovan, Jessica Liu, Lei Qin, Alexandra Garancher, Kun-Wei Liu, Sheila Mansouri, Betty Luu, Yuan Yao Thompson, Vijay Ramaswamy, John Peacock, Hamza Farooq, Patryk Skowron, David J H Shih, Angela Li, Sherine Ensan, Clinton S Robbins, Myron Cybulsky, Siddhartha Mitra, Yussanne Ma, Richard Moore, Andy Mungall, Yoon-Jae Cho, William A Weiss, Jennifer A Chan, Cynthia E Hawkins, Maura Massimino, Nada Jabado, Michal Zapotocky, David Sumerauer, Eric Bouffet, Peter Dirks, Uri Tabori, Poul H B Sorensen, Priscilla K Brastianos, Kenneth Aldape, Steven J M Jones, Marco A Marra, James R Woodgett, Robert J Wechsler-Reya, Daniel W Fults, Michael D Taylor
While the preponderance of morbidity and mortality in medulloblastoma patients are due to metastatic disease, most research focuses on the primary tumor due to a dearth of metastatic tissue samples and model systems. Medulloblastoma metastases are found almost exclusively on the leptomeningeal surface of the brain and spinal cord; dissemination is therefore thought to occur through shedding of primary tumor cells into the cerebrospinal fluid followed by distal re-implantation on the leptomeninges. We present evidence for medulloblastoma circulating tumor cells (CTCs) in therapy-naive patients and demonstrate in vivo, through flank xenografting and parabiosis, that medulloblastoma CTCs can spread through the blood to the leptomeningeal space to form leptomeningeal metastases...
February 22, 2018: Cell
https://www.readbyqxmd.com/read/29474504/the-first-in-vivo-multiparametric-comparison-of-different-radiation-exposure-biomarkers-in-human-blood
#3
Ales Tichy, Sylwia Kabacik, Grainne O'Brien, Jaroslav Pejchal, Zuzana Sinkorova, Adela Kmochova, Igor Sirak, Andrea Malkova, Caterina Gomila Beltran, Juan Ramon Gonzalez, Jakub Grepl, Matthaeus Majewski, Elizabeth Ainsbury, Lenka Zarybnicka, Jana Vachelova, Alzbeta Zavrelova, Marie Davidkova, Marketa Markova Stastna, Michael Abend, Eileen Pernot, Elisabeth Cardis, Christophe Badie
The increasing risk of acute large-scale radiological/nuclear exposures of population underlines the necessity of developing new, rapid and high throughput biodosimetric tools for estimation of received dose and initial triage. We aimed to compare the induction and persistence of different radiation exposure biomarkers in human peripheral blood in vivo. Blood samples of patients with indicated radiotherapy (RT) undergoing partial body irradiation (PBI) were obtained soon before the first treatment and then after 24 h, 48 h, and 5 weeks; i...
2018: PloS One
https://www.readbyqxmd.com/read/29474365/ultra-fast-vitrification-of-patient-derived-circulating-tumor-cell-lines
#4
Rebecca D Sandlin, Keith H K Wong, Shannon N Tessier, Anisa Swei, Lauren D Bookstaver, Bennett E Ahearn, Shyamala Maheswaran, Daniel A Haber, Shannon L Stott, Mehmet Toner
Emerging technologies have enabled the isolation and characterization of rare circulating tumor cells (CTCs) from the blood of metastatic cancer patients. CTCs represent a non-invasive opportunity to gain information regarding the primary tumor and recent reports suggest CTCs have value as an indicator of disease status. CTCs are fragile and difficult to expand in vitro, so typically molecular characterization must be performed immediately following isolation. To ease experimental timelines and enable biobanking, cryopreservation methods are needed...
2018: PloS One
https://www.readbyqxmd.com/read/29473838/-nano-ginseng-for-enhanced-cytotoxicity-against-cancer-cells
#5
Lin Dai, Weiyan Zhu, Chuanling Si, Jiandu Lei
Panax ginseng has high medicinal and health values. However, the various and complex components of ginseng may interact with each other, thus reducing and even reversing therapeutic effects. In this study, we designed and fabricated a novel "nano-ginseng" with definite ingredients, ginsenoside Rb1/protopanaxadiol nanoparticles (Rb1/PPD NPs), completely based on the protopanaxadiol-type extracts. The optimized nano-formulations demonstrated an appropriate size (~110 nm), high drug loading efficiency (~96...
February 23, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29473753/macrophage-membrane-coated-nanoparticles-for-tumor-targeted-chemotherapy
#6
Yu Zhang, Kaimin Cai, Chao Li, Qin Guo, Qinjun Chen, Xi He, Lisha Liu, Yujie Zhang, Yifei Lu, Xinli Chen, Tao Sun, Yongzhuo Huang, Jianjun Cheng, Chen Jiang
Various delivery vectors have been integrated within biologically derived membrane systems to extend their residential time and reduce their reticuloendothelial system (RES) clearance during systemic circulation. However, rational design is still needed to further improve the in situ penetration efficiency of chemo-drug-loaded membrane delivery-system formulations and their release profiles at the tumor site. Here, a macrophage-membrane-coated nanoparticle is developed for tumor-targeted chemotherapy delivery with a controlled release profile in response to tumor microenvironment stimuli...
February 23, 2018: Nano Letters
https://www.readbyqxmd.com/read/29473730/non-catalytic-endosialidase-enables-surface-capture-of-small-cell-lung-cancer-cells-utilizing-strong-dendrimer-mediated-enzyme-glycoprotein-interactions
#7
Hao-Jui Hsu, Helena Palka-Hamblin, Gaurang P Bhide, Ja Hye Myung, Michael Cheong, Karen J Colley, Seungpyo Hong
Enumeration of circulating tumor cells (CTCs) of small-cell lung cancer (SCLC) patients has been shown to predict the disease progress and long-term survival. Most CTC detection methods rely on epithelial surface markers, such as epithelial cell adhesion molecule (EpCAM). However, this marker in SCLC is reported to be often downregulated after a variety of phenotypic changes, which impairs the reliability of EpCAM-based CTC detections. In this regard, the development of an alternative CTC detection method involving different CTC surface markers is in demand...
February 23, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29472915/endothelial-cell-focal-adhesion-regulates-transendothelial-migration-and-subendothelial-crawling-of-t-cells
#8
Jaehyun Lee, Kwang Hoon Song, Taeyeob Kim, Junsang Doh
Leukocytes circulating in the blood stream leave out of blood vessels and infiltrate into inflamed tissues to perform immune responses. Endothelial cells (ECs) lining interior of the post-capillary venules regulate various steps of leukocyte extravasation. In response to inflammatory signals, ECs upregulate adhesion molecules and produce/present chemokines to support firm adhesion and intraluminal crawling of leukocytes. They also remodel junctions to facilitate leukocyte transendothelial migration (TEM). While roles of apical/lateral components of EC layers in regulating leukocyte extravasation have been extensively investigated, relatively little attention has been paid to the basal part of EC layers comprising subendothelial spaces...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29472358/circulating-tumor-dna-as-a-liquid-biopsy-in-plasma-cell-dyscrasias
#9
Bernhard Gerber, Martina Manzoni, Valeria Spina, Alessio Bruscaggin, Marta Lionetti, Sonia Fabris, Marzia Barbieri, Gabriella Ciceri, Alessandra Pompa, Gabriela Forestieri, Erika Lerch, Paolo Servida, Francesco Bertoni, Emanuele Zucca, Michele Ghielmini, Agostino Cortelezzi, Franco Cavalli, Georg Stussi, Luca Baldini, Davide Rossi, Antonino Neri
No abstract text is available yet for this article.
February 22, 2018: Haematologica
https://www.readbyqxmd.com/read/29470068/a-potent-epha2-agonistic-peptide-drug-conjugate-reduces-circulating-cancer-cells-and-metastases-in-breast-cancer-models
#10
Ahmed F Salem, Si Wang, Sandrine Billet, Jie-Fu Chen, Parima Udompholkul, Luca Gambini, Carlo Baggio, Hsian-Rong Tseng, Edwin M Posadas, Neil A Bhowmick, Maurizio Pellecchia
EphA2 overexpression has been associated with metastasis of multiple cancer types, including melanoma, ovarian, prostate, lung, and breast cancer. We have recently proposed to develop peptide-drug conjugates (PDCs) using agonistic EphA2-targeting agents such as the YSA peptide or its optimized version 123B9. While our studies indicated that YSA- or 123B9-drug conjugates can selectively deliver cytotoxic drugs to cancer cells in vivo, the relatively low cellular agonistic activity (high micromolar) of the agents for the EphA2 receptor remained a limiting factor to warrant further development of these PDCs in the clinic...
February 22, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29468874/shape-engineering-boosts-magnetic-mesoporous-silica-nanoparticles-based-isolation-and-detection-of-circulating-tumor-cells
#11
Zhimin Chang, Zheng Wang, Juan Yue, Hao Xing, Li Li, Mingfeng Ge, Mingqiang Li, Huize Yan, Hanze Hu, Dan Shao, Qiaobing Xu, Dayong Jin, Wen-Fei Dong
Magnetic mesoporous silica nanoparticles (M-MSNs) are attractive candidates for the immuno-magnetic isolation and detection of circulating tumor cells (CTCs). Understanding of the interactions between the effect of the shape of M-MSNs and CTCs is crucial to maximize the binding capacity and capture efficiency as well as to facilitate the sensitivity and efficiency of detection. In this work, fluorescent M-MSNs were rationally designed with sphere and rod morphologies while retaining their robust fluorescence and uniform surface functionality...
February 22, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29467948/challenges-in-using-liquid-biopsies-for-gene-expression-profiling
#12
Tania B Porras, Pushpinder Kaur, Alexander Ring, Naomi Schechter, Julie E Lang
Circulating tumor cells (CTCs) have potential utility as a surrogate biomarker of tumor biology via a liquid biopsy. The aim of this study was to evaluate if the nCounter NanoString assay could be used for accurate gene expression profiling of CTCs using the PAM50 research-use-only CodeSet. Analysis was performed on CTCs isolated by the ANGLE Parsortix system from healthy blood spiked with the breast cancer cell lines Hs578T, SkBr3, MDA-MB-231 or MCF7. Using cell lines as gold standard positive controls and Parsortix processed blood without spiking (unspiked) as negative controls, we found an average of 12 significantly differentially expressed genes among spiked samples versus unspiked controls...
January 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29467936/dynamic-changes-in-cd44v-positive-cells-after-preoperative-anti-her2-therapy-and-its-correlation-with-pathologic-complete-response-in-her2-positive-breast-cancer
#13
Teruo Yamauchi, Jose Rodrigo Espinosa Fernandez, Chiyo K Imamura, Hideko Yamauchi, Hiromitsu Jinno, Maiko Takahashi, Yuko Kitagawa, Seigo Nakamura, Bora Lim, Savitri Krishnamurthy, James M Reuben, Diane Liu, Debasish Tripathy, Helen Chen, Naoko Takebe, Hideyuki Saya, Naoto T Ueno
Chemotherapy has been reported to increase the proportion of cancer stem cells (CSCs) and to promote epithelial-mesenchymal transition (EMT) phenotype changes. Anti-HER2 therapy may provide a strategy for eliminating CSC and EMT, which contribute to therapeutic resistance. No study has determined the changes in the quantity or characteristics of CSCs or circulating tumor cells (CTCs) with EMT phenotype during preoperative anti-HER2 therapy, and whether these changes correlate to response to dual anti-HER2 therapy...
January 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29467871/abnormal-expression-of-circulating-and-tumor-infiltrating-carcinoembryonic-antigen-related-cell-adhesion-molecule-1-in-patients-with-glioma
#14
Jinhu Li, Xiaodong Liu, Yijun Duan, Hongqin Wang, Wen Su, Yazhou Wang, Guotao Zhuang, Yimin Fan
Glioma, the most prevalent primary tumor of the central nervous system, is known to evade immune surveillance and escape immune attacks by inducing immunosuppression. The homophilic interactions of the carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) serve a critical function in immunoregulation. In the present study, the expression levels of CEACAM1 in peripheral blood mononuclear cells and tumor-infiltrating lymphocytes (TILs) from patients with gliomas were assessed. Furthermore, associations between CEACAM1 expression and multiple clinicopathological characteristics in patients with gliomas were analyzed...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29467551/cryopreservation-for-delayed-circulating-tumor-cell-isolation-is-a-valid-strategy-for-prognostic-association-of-circulating-tumor-cells-in-gastroesophageal-cancer
#15
Daniel Brungs, David Lynch, Alison Ws Luk, Elahe Minaei, Marie Ranson, Morteza Aghmesheh, Kara L Vine, Martin Carolan, Mouhannad Jaber, Paul de Souza, Therese M Becker
AIM: To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells (PBMCs) for prognostic circulating tumor cell (CTC) detection in gastroesophageal cancer. METHODS: Using 7.5 mL blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the IsoFlux platform. Paired specimens taken during the same blood draw ( n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs...
February 21, 2018: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29464943/molecular-profiling-of-pooled-circulating-tumor-cells-from-prostate-cancer-patients-using-dual-antibody-functionalized-microfluidic-device
#16
Changqing Yin, Yuhui Wang, Jia Ji, Bo Cai, Hao Chen, Zhonghua Yang, Kun Wang, Changliang Luo, Wu-Wen Zhang, Chunhui Yuan, Fubing Wang
Here, we constructed a novel dual-antibody-functionalized microfluidic device by employing antibodies against PSMA and EpCAM to capture CTCs from PCa patients in order to capture multiple subpopulations of CTCs at different metastatic stages. In vitro experiments with dual capture system for capturing both LnCAP and LnCAP-EMT cells have shown significantly enhanced capture efficiency as compared to that of EpCAM single capture system. Furthermore, dual capture system could successfully identify CTCs in 20 out of 24 (83...
February 21, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29464405/the-role-of-circulating-tumor-dna-in-renal-cell-carcinoma
#17
REVIEW
Paulo G Bergerot, Andrew W Hahn, Cristiane Decat Bergerot, Jeremy Jones, Sumanta Kumar Pal
Next-generation sequencing (NGS) of circulating tumor DNA (ctDNA) is a novel technology that can complement tumor tissue NGS and has the potential to influence diagnosis and treatment of both localized and metastatic renal cell carcinoma (mRCC). ctDNA NGS is an attractive alternative to tumor tissue NGS because it circumvents the need for repeated, invasive tissue biopsies while providing a contemporary mutational profile of a patient's tumors. While the role of ctDNA NGS in non-small cell lung cancer and colorectal cancer is well established, studies of ctDNA NGS in mRCC are only hypothesis-generating to date...
February 20, 2018: Current Treatment Options in Oncology
https://www.readbyqxmd.com/read/29464100/pancreatic-cancer-disease-dynamics-tumor-biology-and-the-role-of-the-microenvironment
#18
REVIEW
Daniel Ansari, Helmut Friess, Monika Bauden, Johan Samnegård, Roland Andersson
Pancreatic cancer is known for its propensity to metastasize. Recent studies have challenged the commonly held belief that pancreatic cancer is a stepwise process, where tumor cells disseminate late in primary tumor development. Instead it has been suggested that pancreatic tumor cells may disseminate early and develop independently and in parallel to the primary tumor. Circulating tumor cells can be found in most patients with pancreatic cancer, even in those with localized stage. Also, recent phylogenetic analyses have revealed evidence for a branched evolution where metastatic lineages can develop early in tumor development...
January 19, 2018: Oncotarget
https://www.readbyqxmd.com/read/29463222/filtration-based-assessment-of-ctcs-and-cellsearch%C3%A2-based-assessment-are-both-powerful-predictors-of-prognosis-for-metastatic-breast-cancer-patients
#19
Hanna Huebner, Peter A Fasching, Walter Gumbrecht, Sebastian Jud, Claudia Rauh, Mark Matzas, Peter Paulicka, Katja Friedrich, Michael P Lux, Bernhard Volz, Paul Gass, Lothar Häberle, Franziska Meier-Stiegen, Andreas Hartkopf, Hans Neubauer, Katrin Almstedt, Matthias W Beckmann, Tanja N Fehm, Matthias Ruebner
BACKGROUND: The assessment of circulating tumor cells (CTCs) has been shown to enable monitoring of treatment response and early detection of metastatic breast cancer (MBC) recurrence. The aim of this study was to compare a well-established CTC detection method based on immunomagnetic isolation with a new, filtration-based platform. METHODS: In this prospective study, two 7.5 ml blood draws were obtained from 60 MBC patients and CTC enumeration was assessed using both the CellSearch® and the newly developed filtration-based platform...
February 20, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29463155/circulating-cell-free-dna-of-methylated-insulin-like-growth-factor-binding-protein-7-predicts-a-poor-prognosis-in-hepatitis-b-virus-associated-hepatocellular-carcinoma-after-hepatectomy
#20
Feng Li, Chen-Yang Qiao, Shuai Gao, Yu-Chen Fan, Long-Yan Chen, Kai Wang
BACKGROUND AND AIM: The initiation and progression of hepatocellular carcinoma (HCC) is a multistage process involving a variety of changes at the gene level. Insulin-like growth factor binding protein 7 (IGFBP7) methylation plays a crucial role in HCC development. The main purpose of this study was to investigate the relationship between oxidative stress, DNA methyltransferases (DNMTs) expression and IGFBP7 methylation, and to evaluate the prognostic value of serum IGFBP7 methylation status in patients with HCC after hepatectomy...
February 20, 2018: Free Radical Research
keyword
keyword
117154
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"