keyword
MENU ▼
Read by QxMD icon Read
search

Kpc klebsiella

keyword
https://www.readbyqxmd.com/read/29452198/microbial-infection-pattern-pathogenic-features-and-resistance-mechanism-of-carbapenem-resistant-gram-negative-bacilli-during-long-term-hospitalization
#1
Shuxian Wen, Donghua Feng, Zerong Lu, Junyan Liu, Brian M Peters, Hailing Tang, Danhong Su, Yong-Ping Lin, Ling Yang, Zhenbo Xu, Mark E Shirtliff, Dingqiang Chen
BACKGROUND: Carbapenem-resistant Gram-negative bacilli (GNB) have become an important cause of nosocomial infections of hospitalized patients. METHODS: To investigate the microbial infection patterns and molecular epidemiology characteristics of the carbapenem-resistant GNB isolates from a long-term hospitalized patient, antimicrobial susceptibility testing, phenotypic screening test for carbapenemase production, PCR screening and DNA sequencing of carbapenemase genes, repetitive extragenic palindromic sequence-based PCR (REP-PCR), multilocus sequencing typing (MLST) and genetic environment analysis were performed...
February 13, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29444730/outbreak-of-st395-kpc-producing-klebsiella-pneumoniae-in-a-neonatal-intensive-care-unit-in-palermo-italy
#2
Carmelo M Maida, Celestino Bonura, Daniela M Geraci, Giorgio Graziano, Alessandra Carattoli, Angelo Rizzo, Maria V Torregrossa, Davide Vecchio, Mario Giuffrè
No abstract text is available yet for this article.
February 15, 2018: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/29427523/meropenem-and-vaborbactam-stepping-up-the-battle-against-carbapenem-resistant-enterobacteriaceae
#3
Sarah Christina Jane Jorgensen, Michael Joseph Rybak
Vaborbactam (VAB; formerly RPX7009) is a novel beta-lactamase inhibitor based on a cyclic boronic acid pharmacophore with potent inhibitory activity against Ambler class A and C beta-lactamases. It has been co-formulated with meropenem to restore its activity against Klebsiella pneumoniae carbapenemases (KPC). VAB does not inhibit class B or D carbapenemases, nor does it improve the activity of meropenem against multidrug-resistant non-fermenting Gram-negative bacilli, notably Acinetobacter spp. and Pseudomonas aeruginosa...
February 10, 2018: Pharmacotherapy
https://www.readbyqxmd.com/read/29424684/genome-analysis-of-clinical-multilocus-sequence-type-11-klebsiella-pneumoniae-from-china
#4
Ning Dong, Rong Zhang, Lizhang Liu, Ruichao Li, Dachuan Lin, Edward Wai-Chi Chan, Sheng Chen
The increasing prevalence of KPC-producing Klebsiella pneumoniae strains in clinical settings has been largely attributed to dissemination of organisms of specific multilocus sequence types, such as ST258 and ST11. Compared with the ST258 clone, which is prevalent in North America and Europe, ST11 is common in China but information regarding its genetic features remains scarce. In this study, we performed detailed genetic characterization of ST11 K. pneumoniae strains by analyzing whole-genome sequences of 58 clinical strains collected from diverse geographic locations in China...
February 8, 2018: Microbial Genomics
https://www.readbyqxmd.com/read/29422888/klebsiella-pneumoniae-carbapenemase-producers-in-south-korea-between-2013-and-2015
#5
Eun-Jeong Yoon, Jung Ok Kim, Dokyun Kim, Hyukmin Lee, Ji Woo Yang, Kwang Jun Lee, Seok Hoon Jeong
Between 2014 and 2015, the Klebsiella pneumoniae carbapenemase (KPC) was becoming endemic in South Korea. To assess this period of transition, we analyzed KPC producers in terms of molecular epidemiology. A total of 362 KPC-producing Enterobacteriaceae strains, including one from 2013, 13 from 2014, and 348 from 2015, were actively collected from 60 hospitals throughout the peninsula. Subtypes of KPC were determined by PCR and direct sequencing, and isotypes of Tn4401 (the transposon flanking the blaKPC gene) were specified by PCR using isotype-specific primers and direct sequencing...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29422290/genetic-variability-of-carbapenemase-kpc-producing-klebsiella-pneumoniae-isolated-at-different-states-in-venezuela
#6
Nirvia Margot Cuaical-Ramos, Marynes Montiel, Daniel Marcano Zamora
INTRODUCTION: In Venezuela, there have been some reports of carbapenemase KPC-producing Klebsiella pneumoniae. Nevertheless, since the first report in 2008, only a few studies have been done on their molecular epidemiology in this country. METHODS: The aims of this study were to detect extended-spectrum betalactamase (ESBL)-producing (blaTEM and blaCTM-M-1) and to determine the genetic relationship between 30 isolates of carbapenemase KPC-producing K. pneumoniae taken from patients at eleven health centers in different states of Venezuela from January 2008 to December 2012, using pulsed-field gel electrophoresis (PFGE)...
February 5, 2018: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29416121/in-vitro-activity-of-minocycline-combined-with-aminoglycosides-against-klebsiella-pneumoniae-carbapenemase-producing-k-pneumoniae
#7
Ni Wentao, Li Guobao, Zhao Jin, Cui Junchang, Wang Rui, Gao Zhancheng, Liu Youning
This study assessed the in vitro antibacterial activity of minocycline-aminoglycoside combination against Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae. Seventy non-duplicate clinical isolates of KPC-producing K. pneumoniae were collected from patients with bloodstream infections. The synergistic activity of minocycline-aminoglycoside combination was studied by the checkerboard method and time-kill assays in strains with different susceptibilities, and the mutant prevention concentration (MPC) and mutant selection window (MSW) of drugs alone and in combination were determined...
February 7, 2018: Journal of Antibiotics
https://www.readbyqxmd.com/read/29415120/impact-of-the-st101-clone-on-fatality-among-patients-with-colistin-resistant-klebsiella-pneumoniae-infection
#8
Fusun Can, Sirin Menekse, Pelin Ispir, Nazli Atac, Ozgur Albayrak, Tuana Demir, Doruk Can Karaaslan, Salih Nafiz Karahan, Mahir Kapmaz, Ozlem Kurt Azap, Funda Timurkaynak, Serap Simsek Yavuz, Seniha Basaran, Fugen Yoruk, Alpay Azap, Safiye Koculu, Nur Benzonana, Nathan A Lack, Mehmet Gönen, Onder Ergonul
Objectives: We describe the molecular characteristics of colistin resistance and its impact on patient mortality. Methods: A prospective cohort study was performed in seven different Turkish hospitals. The genotype of each isolate was determined by MLST and repetitive extragenic palindromic PCR (rep-PCR). Alterations in mgrB were detected by sequencing. Upregulation of pmrCAB, phoQ and pmrK was quantified by RT-PCR. mcr-1 and the genes encoding OXA-48, NDM-1 and KPC were amplified by PCR...
February 3, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29412797/frequency-determination-of-carbapenem-resistant-klebsiella-pneumoniae-crkp-isolated-from-hospitals-in-isfahan-of-iran-and-evaluation-of-synergistic-effect-of-colistin-and-meropenem-on-them
#9
Leila Gheitani, Hossein Fazeli, Sharareh Moghim, Bahram Nasr Isfahani
Overuse and misuse of Carbapenems among Klebsiella pneumoniae isolates have caused Carbapenem-Resistant Klebsiella Pneumonia (CRKP) during recent years. Colistin is one of the last available options, and there are increasing concerns about the dosage and resistance to this agent in long-term monotherapies. This study was designed to identification of carbapenemase producing isolates of K. pneumoniae via phenotypic and genotypic methods as well as evaluation of colistin-meropenem combination therapy potential...
January 31, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29409044/intensive-care-unit-wastewater-interventions-to-prevent-transmission-of-multi-species-klebsiella-pneumoniae-carbapenemase-kpc-producing-organisms
#10
Amy J Mathers, Kasi Vegesana, Ian German Mesner, Katie E Barry, Aaron Pannone, Josh Baumann, Derrick W Crook, Nicole Stoesser, Shireen Kotay, Joanne Carroll, Costi D Sifri
Background: Increasing prevalence of nosocomial carbapenemase-producing Enterobacteriaceae is a concern; however, the role of the environment in multispecies outbreaks remains poorly understood. There is increasing recognition that hospital wastewater plumbing may play a role. Methods: Covers were installed on all hoppers in adult intensive care units (ICUs) of a university hospital; in the surgical ICU (SICU) both hopper covers and sink trap heating/vibration devices were installed...
February 2, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29399114/mechanism-for-carbapenem-resistance-of-clinical-enterobacteriaceae-isolates
#11
Yafei Ye, Lijuan Xu, Yanping Han, Zhe Chen, Cailin Liu, Liang Ming
Carbapenemase-producing 'super bacteria', particularly NDM-1 and its variants, have become a major public health concern worldwide. The present study aimed to explore the molecular mechanism for carbapenem resistance of clinical Enterobacteriaceae isolates. Seventy-eight non-repeated Enterobacteriaceae strains resistant to any carbapenem were screened at the First Affiliated Hospital of Zhengzhou University (Zhengzhou, China) between December 2011 and December 2015. Outer membrane porin (OMP) proteins were detected using SDS-PAGE...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29391471/detection-of-16s-rrna-and-kpc-genes-from-complex-matrix-utilizing-a-molecular-inversion-probe-assay-for-next-generation-sequencing
#12
Christopher P Stefan, Adrienne T Hall, Timothy D Minogue
Targeted sequencing promises to bring next-generation sequencing (NGS) into routine clinical use for infectious disease diagnostics. In this context, upfront processing techniques, including pathogen signature enrichment, must amplify multiple targets of interest for NGS to be relevant when applied to patient samples with limited volumes. Here, we demonstrate an optimized molecular inversion probe (MIP) assay targeting multiple variable regions within the 16S ribosomal gene for the identification of biothreat and ESKAPE pathogens in a process that significantly reduces complexity, labor, and processing time...
February 1, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29386265/rapid-increase-in-the-prevalence-of-carbapenem-resistant-enterobacteriaceae-cre-and-emergence-of-colistin-resistance-gene-mcr-1-in-cre-in-a-hospital-in-henan-china
#13
Yi Li, Qiao-Ling Sun, Yingbo Shen, Yangjunna Zhang, Jun-Wen Yang, Ling-Bin Shu, Hong-Wei Zhou, Yang Wang, Bing Wang, Rong Zhang, Shaolin Wang, Zhangqi Shen
The global spread of carbapenem-resistant Enterobacteriaceae (CRE) is one of the most severe threats to human health in a clinical setting. The recent emergence of plasmid-mediated colistin resistance gene mcr-1 amongst CRE strains greatly compromises the use of colistin as a last resort for the treatment of infections caused by CRE. This study aimed to understand the current epidemiological trends and characteristics of CRE from a large hospital in Henan, the most populous province in China. From 2014--2016, a total of 7,249 Enterobacteriaceae isolates were collected from clinical samples, among which 18...
January 31, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29373768/results-of-the-national-surveillance-of-antimicrobial-resistance-of-enterobacteriaceae-and-gram-negative-bacilli-in-health-care-associated-infections-in-colombia-2012-2014
#14
María Victoria Ovalle, Sandra Yamile Saavedra, María Nilse González, Andrea Melissa Hidalgo, Carolina Duarte, Mauricio Beltrán
INTRODUCTION: The Colombian National Antimicrobial Resistance Monitoring System for the surveillance of healthcare-associated infections was set up to meet this problem in the third quarter of 2012. OBJECTIVE: To describe resistance profiles and laboratory-based surveillance based on the information collected by the System. MATERIALS AND METHODS: We conducted a retrospective and descriptive study of the information notified to the Colombian Public Health Surveillance System (Sivigila), and in the Whonet databases covering the period from July 2012 to December 2014 provided by the primary data-generating units in the country, as well as laboratory surveillance results from 1,642 phenotypic and genotypic tests on carbapenemase isolates (927 from Enterobacteriaceae, 614 from Pseudomonas spp...
December 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29371104/activity-of-ceftazidime-avibactam-against-carbapenemase-producing-enterobacteriaceae-from-urine-samples-obtained-during-the-infection-carbapenem-resistance-evaluation-surveillance-trial-icrest-in-spain
#15
María García-Castillo, Sergio García-Fernández, Rosa Gómez-Gil, Cristina Pitart, Marina Oviaño, Irene Gracia-Ahufinger, Jazmín Díaz-Regañón, Marta Tato, Rafael Cantón
The increasing rates of carbapenemase-producing Enterobacteriaceae (CPE) represent an important threat to health care systems and make treatment of CPE infections a challenge. The aim of the infection-carbapenem resistance evaluation surveillance trial (iCREST) was to determinate the prevalence of CPE in urine specimens in Spain and to evaluate the in vitro activity of ceftazidime-avibactam. Urine specimens (n=11,826) were included and activity of ceftazidime-avibactam and comparators were investigated by broth microdilution in CPE...
January 22, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29358148/first-detection-and-genomics-analysis-of-kpc-2-producing-citrobacter-isolates-from-river-sediments
#16
Hao Xu, Xin Wang, Xiao Yu, Jing Zhang, Lihua Guo, Chen Huang, Xiawei Jiang, Xuewen Li, Youjun Feng, Beiwen Zheng
The wide spread of carbapenemase-producing Enterobacteriaceae (CPE) in the environment is an emerging environmental issue with potentially-serious public health implications. However, carbapenemase-producing Citrobacter from environment has rarely been investigated. Here we report the isolation and comparative genomics of carbapenemase-producing Citrobacter isolates from river sediment in China. Potential CPE was isolated by selective MacConkey agar plates containing 2 mg/L meropenem. The presence of carbapenemase genes was detected by PCR and sequencing...
January 19, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29356380/phenyl-boronic-acids-development-led-to-validated-leads-active-in-clinical-strains-overexpressing-kpc-2-a-step-against-bacterial-resistance
#17
Giuseppe Celenza, Mattia Vicario, Pierangelo Bellio, Linciano Pasquale, Mariagrazia Perilli, Antonio Oliver, Jesus Blazquez, Laura Cendron, Donatella Tondi
The emergence and dissemination of multi drug resistant (MDR) pathogens resistant to near all available antibiotics poses a significant threat in clinical therapy. Among them, Klebsiella pneumoniae clinical isolates overexpressing KPC-2 carbapenemase are the most worrisome, extending bacterial resistance to last resort carbapenems. In this study we investigate the molecular recognition requirements in KPC-2 active site by small phenyl boronic acid derivatives. Four new phenyl boronic acid derivatives were designed and tested vs KPC-2...
January 22, 2018: ChemMedChem
https://www.readbyqxmd.com/read/29336224/treatment-of-a-klebsiella-pneumoniae-kpc-cellulitis-and-gut-decolonization-with-ceftazidime-avibactam-in-a-migrant-from-libya
#18
Novella Carannante, Carlo Pallotto, Mariano Bernardo, Giovanni Di Caprio, Carlo Tascini
KPC-producing Klebsiella pneumoniae (KPC-Kp) is nowadays a global concern. Ceftazidime/avibactam is the most promising novel antibiotic combination available at the moment. We describe the case of a migrant with no risk factors for an infection due to multidrug resistant bacteria. He suffered from a KPC-Kp cellulitis and was treated with a ceftazidime/avibactam-meropenem-fosfomycin combination that not only eradicated the infection but also decontaminated his gut. Ceftazidime/avibactam-based treatment can be useful also in decontamination procedures...
January 16, 2018: Journal of Chemotherapy
https://www.readbyqxmd.com/read/29319505/kpc-2-producing-klebsiella-pneumoniae-st11-in-a-children-s-hospital-in-poland
#19
Monika Machulska, Anna Baraniak, Iwona Żak, Katarzyna Bojarska, Dorota Żabicka, Iwona Sowa-Sierant, Waleria Hryniewicz, Marek Gniadkowski
Four Klebsiella pneumoniae isolates from children hospitalized over 10 months in an intensive care unit in a children's teaching hospital in Poland were analyzed. All of the isolates belonged to a single pulsotype and sequence type (ST) 11, and produced the KPC-2 carbapenemase and extended-spectrum β-lactamase (ESBL) CTX-M-15. They were resistant to a variety of antimicrobials, and their β-lactam resistance patterns were typical for KPC producers. This is one of few cases of identification of KPC (or carbapenemase)-producing K...
September 27, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29312831/translating-genomics-into-practice-for-real-time-surveillance-and-response-to-carbapenemase-producing-enterobacteriaceae-evidence-from-a-complex-multi-institutional-kpc-outbreak
#20
Jason C Kwong, Courtney R Lane, Finn Romanes, Anders Gonçalves da Silva, Marion Easton, Katie Cronin, Mary Jo Waters, Takehiro Tomita, Kerrie Stevens, Mark B Schultz, Sarah L Baines, Norelle L Sherry, Glen P Carter, Andre Mu, Michelle Sait, Susan A Ballard, Torsten Seemann, Timothy P Stinear, Benjamin P Howden
Background: Until recently, Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae were rarely identified in Australia. Following an increase in the number of incident cases across the state of Victoria, we undertook a real-time combined genomic and epidemiological investigation. The scope of this study included identifying risk factors and routes of transmission, and investigating the utility of genomics to enhance traditional field epidemiology for informing management of established widespread outbreaks...
2018: PeerJ
keyword
keyword
117151
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"