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Kpc klebsiella

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https://www.readbyqxmd.com/read/29688339/4-5-years-within-patient-evolution-of-a-colistin-resistant-kpc-producing-klebsiella-pneumoniae-st258
#1
Agnès B Jousset, Rémy A Bonnin, Isabelle Rosinski-Chupin, Delphine Girlich, Gaëlle Cuzon, Nicolas Cabanel, Hélène Frech, Eric Farfour, Laurent Dortet, Philippe Glaser, Thierry Naas
Background: KPC-producing Klebsiella pneumoniae (KPC-Kp) have emerged globally over the last decade as a major nosocomial pathogen that threatens patient's care. These highly resistant bacteria are mostly associated with a single Kp clonal group (CG) CG258, but the reasons for its host and hospital adaptation remain largely unknown. Methods: We analyzed the in vivo evolution of a colistin-resistant KPC-Kp-CG258 strain that contaminated a patient following an endoscopy, and was responsible for a fatal bacteraemia 4...
April 21, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29682477/identification-of-extended-spectrum-beta-lactamases-ampc-and-carbapenemase-production-among-isolates-of-escherichia-coli-in-north-indian-tertiary-care-centre
#2
Uma Chaudhary, Shipra Agarwal, Kausalya Raghuraman
Introduction: Identification of Extended spectrum beta lactamases (ESBL), AmpC production and carbapenemase production among isolates of Escherichia coli , helps clinician to rationalize the choice of antibiotics. However, there is a lack of simple and effective method for simultaneous identification of these beta lactamases. Aim: To determine the concurrent production of beta lactamases using twelve disc method on E. coli isolates. Materials and Methods: A total of 200 multidrug resistant E...
April 2018: Avicenna Journal of Medicine
https://www.readbyqxmd.com/read/29679645/sequencing-of-pt5282-ctxm-p13190-kpc-and-p30860-nr-and-comparative-genomics-analysis-of-incx8-plasmids
#3
Haihong Fang, Jiao Feng, Yuanhong Xu, Ying Zhang, Zhe Zhan, Zhe Yin, Xiongbo Duan, Bo Gao, Yigang Tong, Jinglin Wang, Yanping Luo, Erhei Dai, Dongsheng Zhou
This study proposed a replicon-based scheme for typing IncX into nine separately clustering subgroups, including IncX1α, IncX1β and IncX2-8. The complete nucleotide sequences of three IncX8 plasmids, namely pT5282-CTXM and p30860-NR from Enterobacter cloacae and p13190-KPC from Klebsiella pneumoniae, were determined and compared with all the other two previously sequenced IncX8 plasmids pCAV1043-58 and pCAV1741-16. These five plasmids possessed conserved IncX8 backbones with limited genetic variations with respect to gene content and organization, and each of them carried one or three accessory modules that harbored resistance markers and metabolic gene clusters as well as transposons, insertion sequence (IS)-based transposition units and miniature inverted repeat transposable elements, indicating that the relatively small IncX8 backbones were able to integrate various foreign genetic contents...
April 18, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29679603/chromogenic-culture-media-or-rapid-immunochromatographic-test-which-is-better-for-detecting-klebsiella-pneumoniae-that-produce-oxa-48-and-can-they-be-used-in-blood-and-urine-specimens
#4
Ozlem Genc, Evrim Aksu
Our goal was to compare a rapid test (OXA-48K-SeT) and four different chromogenic media (CHROMagar KPC, CHROMagar mSuperCARBA, ChromID Carba and ChromID OXA-48) for the detection of OXA-48 producing Klebsiella pneumoniae isolates and spiked urine/blood samples with these bacteria. In total 100 K.pneumoniae isolates, including 60 OXA-48 positive, 15 other carbapenemase producing, 15 Extended spectrum betalactamases (ESBL) positive and 10 carbapenem sensitive K.pneumoniae were included in the study. After all samples were inoculated into all chromogenic media, temocillin discs were placed onto the media...
April 18, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29675006/the-rapid-emergence-of-tigecycline-resistance-in-bla-kpc-2-harboring-klebsiella-pneumoniae-as-mediated-in-vivo-by-mutation-in-teta-during-tigecycline-treatment
#5
Xiaoxing Du, Fang He, Qiucheng Shi, Feng Zhao, Juan Xu, Ying Fu, Yunsong Yu
Tigecycline is one of the last resort treatments for carbapenem-resistant Klebsiella pneumoniae (CRKP) infections. Tigecycline resistance often occurs during the clinical treatment of CRKP, yet its mechanism has still not been clearly elucidated. This study presents an analysis of a tigecycline resistance mechanism that developed in clinical isolates from a 56-year-old female patient infected with CRKP during tigecycline treatment. Consecutive clonal consistent K. pneumoniae isolates were obtained during tigecycline treatment...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29673980/photoactivated-2-3-distyrylindoles-kill-multi-drug-resistant-bacteria
#6
Leslie Edwards, Danielle Turner, Cody Champion, Megha Khandelwal, Kailee Zingler, Cassidy Stone, Ruwini D Rajapaksha, Jing Yang, Mahinda I Ranasinghe, Alexander Kornienko, Liliya V Frolova, Snezna Rogelj
Compounds based on the 2,3-distyrylindole scaffold were found to exhibit bactericidal properties upon irradiation with white light. At the concentration of 1 μM, the lead compound 1 completely (ca. 109  CFU/mL) eradicated such Gram-positive organisms as S. aureus (MRSA, MSSA), E. faecalis (VRE), S. pyogenes and S. mutans when irradiated with white light for 2 min. At the concentration of 5 μM and in the presence of polymyxin E at non-bactericidal 1.25 μg/mL concentration, 1 also showed a 7-log to 9-log reductions in bacterial counts of such Gram-negative organisms as multi-drug resistant (MDR) A...
April 3, 2018: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29673319/carbapenemase-producing-enterobacteriaceae-in-mexico-report-of-seven-non-clonal-cases-in-a-pediatric-hospital
#7
Alejandra Aquino-Andrade, Jocelin Merida-Vieyra, Eduardo Arias de la Garza, Patricia Arzate-Barbosa, Agustín De Colsa Ranero
BACKGROUND: Carbapenemases-producing Enterobacteriaceae (CPE) are a worldwide public health emergency. In Mexico, reports of CPE are limited, particularly in the pediatric population. Here, we describe the clinical, epidemiological, and molecular characteristics of seven consecutive cases in a third-level pediatric hospital in Mexico City over a four-month period during 2016. RESULTS: The Enterobacteriaceae identified were three Escherichia coli strains (producing OXA-232, NDM-1 and KPC-2), two Klebsiella pneumoniae strains (producing KPC-2 and NDM-1), one Klebsiella oxytoca strain producing OXA-48 and one Enterobacter cloacae strain producing NDM-1...
April 19, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29672169/functional-bla-kpc-2-sequences-are-present-in-u-s-beef-cattle-feces-regardless-of-antibiotic-use
#8
Amit Vikram, John W Schmidt
Carbapenems are classified as critically important antibiotics since they are employed when resistant Gram-negative bacterial infections fail to respond to other antibiotic therapies. Carbapenem-resistant bacteria (CRB) were traditionally understood to be rare in the U.S. food-producing animals. Recently, using quantitative polymerase chain reaction (qPCR), our group detected blaKPC-2 in all 72 metagenomic DNA (mgDNA) samples prepared from the feces of 36 lots of beef cattle "raised without antibiotics" (RWA) and 36 lots raised "conventionally" (CONV)...
April 19, 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29669884/iron-acquisition-and-siderophore-release-by-carbapenem-resistant-sequence-type-258-klebsiella-pneumoniae
#9
Victoria I Holden, Meredith S Wright, Sébastien Houle, Abigail Collingwood, Charles M Dozois, Mark D Adams, Michael A Bachman
Klebsiella pneumoniae is rapidly acquiring resistance to all known antibiotics, including carbapenems. Multilocus sequence type ST258 (sequence type 258), carrying a gene encoding the K. pneumoniae carbapenemase ( bla KPC ) on a transmissible plasmid, is the most prevalent carbapenem-resistant Enterobacteriaceae (CRE) in the United States and has disseminated worldwide. Previously, whole-genome sequencing identified core genome single nucleotide variants that divide ST258 into two distinct clades, ST258a and ST258b...
April 25, 2018: MSphere
https://www.readbyqxmd.com/read/29659861/in-vitro-activity-of-imipenem-relebactam-against-gram-negative-eskape-pathogens-isolated-in-17-european-countries-2015-smart-surveillance-programme
#10
James A Karlowsky, Sibylle H Lob, Krystyna M Kazmierczak, Stephen P Hawser, Sophie Magnet, Katherine Young, Mary R Motyl, Daniel F Sahm
Objectives: Relebactam is an inhibitor of class A β-lactamases, including KPC β-lactamases, and class C β-lactamases, and is currently under clinical development in combination with imipenem. The objective of the current study was to evaluate the in vitro activity of imipenem/relebactam against Gram-negative ESKAPE pathogens (Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp.) submitted by clinical laboratories in 17 European countries to the Study for Monitoring Antimicrobial Resistance Trends (SMART) global surveillance programme in 2015...
April 11, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29657865/stress-adaptive-responses-associated-with-high-level-carbapenem-resistance-in-kpc-producing-klebsiella-pneumoniae
#11
Sheila Adams-Sapper, Adam Gayoso, Lee W Riley
Carbapenem-resistant Enterobacteriaceae (CRE) organisms have emerged to become a major global public health threat among antimicrobial resistant bacterial human pathogens. Little is known about how CREs emerge. One characteristic phenotype of CREs is heteroresistance, which is clinically associated with treatment failure in patients given a carbapenem. Through in vitro whole-transcriptome analysis we tracked gene expression over time in two different strains (BR7, BR21) of heteroresistant KPC-producing Klebsiella pneumoniae, first exposed to a bactericidal concentration of imipenem followed by growth in drug-free medium...
2018: Journal of Pathogens
https://www.readbyqxmd.com/read/29642473/resistance-to-carbapenems-in-non-typhoidal-salmonella-enterica-serovars-from-humans-animals-and-food
#12
REVIEW
Javier Fernández, Beatriz Guerra, M Rosario Rodicio
Non-typhoidal serovars of Salmonella enterica (NTS) are a leading cause of food-borne disease in animals and humans worldwide. Like other zoonotic bacteria, NTS have the potential to act as reservoirs and vehicles for the transmission of antimicrobial drug resistance in different settings. Of particular concern is the resistance to critical "last resort" antimicrobials, such as carbapenems. In contrast to other Enterobacteriaceae (e.g., Klebsiella pneumoniae , Escherichia coli , and Enterobacter , which are major nosocomial pathogens affecting debilitated and immunocompromised patients), carbapenem resistance is still very rare in NTS...
April 8, 2018: Veterinary Sciences
https://www.readbyqxmd.com/read/29623139/rapd-pcr-profile-antibiotic-resistance-prevalence-of-arma-gene-and-detection-of-kpc-enzyme-in-klebsiella-pneumoniae-isolates
#13
Arezoo Saadatian Farivar, Jamileh Nowroozi, Gita Eslami, Azar Sabokbar
The increasing prevalence of multidrug-resistant Klebsiella pneumoniae strains isolated from hospitals shows the limitation of recent antibiotics used for bacterial eradication. In this study, 81 K. pneumoniae isolates were collected from three hospitals in Tehran. Antibiotic susceptibility test showed the highest rates of resistance to cefotaxim (85.5%) and ceftazidime (78.3%), and the lowest rates of resistance were detected for colistin (16.9%), streptomycin (16.8%), and chloroamphenicol (21.7%). Eleven different resistance patterns were observed...
2018: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/29621600/nosocomial-transmission-of-carbapenem-resistant-klebsiella-pneumoniae-in-an-italian-university-hospital-a-molecular-epidemiological-study
#14
Giovanni Sotgiu, Bianca Maria Are, Lucia Pesapane, Alessandra Palmieri, Narcisa Muresu, Andrea Cossu, Marco Dettori, Antonio Azara, Ida Iolanda Mura, Clementina Cocuzza, Stefano Aliberti, Andrea Piana
AIM: To describe the phenotypic and genotypic profiles of Klebsiella pneumonia carbapenemase-producing K. pneumonia (KPC-Kp) strains isolated from patients with invasive infections at an Italian university hospital in order to assess the epidemiological trend. METHODS: An observational prospective study was carried out at the University Hospital of Sassari, Italy, to detect KPC-Kp strains in patients with invasive bacteraemia. Isolates were identified phenotypically; carbapenemase production was assessed using phenotypic and genotypic methods...
April 2, 2018: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29616001/dissemination-of-kpc-2-encoding-incx6-plasmids-among-multiple-enterobacteriaceae-species-in-a-single-chinese-hospital
#15
Bing Li, Jiao Feng, Zhe Zhan, Zhe Yin, Qiyu Jiang, Ping Wei, Xingming Chen, Bo Gao, Jun Hou, Panyong Mao, Weili Wu, Weijun Chen, Yigang Tong, Jinglin Wang, Boan Li, Dongsheng Zhou
Forty-five KPC-producing Enterobacteriaceae strains were isolated from multiple departments in a Chinese public hospital from 2014 to 2015. Genome sequencing of four representative strains, namely Proteus mirabilis GN2, Serratia marcescens GN26, Morganella morganii GN28, and Klebsiella aerogenes E20, indicated the presence of bla KPC-2 -carrying IncX6 plasmids pGN2-KPC, pGN26-KPC, pGN28-KPC, and pE20-KPC in the four strains, respectively. These plasmids were genetically closely related to one another and to the only previously sequenced IncX6 plasmid, pKPC3_SZ...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29615166/phenotypic-identification-frequency-distribution-and-antibiogram-of-carbapenemase-producing-enterobacteriaceae-in-clinical-isolates
#16
Maliha Ansari, Tehmina Munir, Nadia Saad
OBJECTIVE: To differentiate between Ambler class A, B and D of carbapenemase-producing Enterobacteriaceae by using simple phenotypic methods that can be carried out in the laboratory without requiring any specialised techniques. STUDY DESIGN: Cross-sectional study. PLACE AND DURATION OF STUDY: Microbiology Department, Army Medical College, NUST, Islamabad, from November 2015 to November 2016. METHODOLOGY: Clinical specimens were subjected to identification of Enterobacteriaceae by colony morphology and API 20 E...
April 2018: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/29610206/st273-carbapenem-resistant-klebsiella-pneumoniae-carrying-bla-ndm-1-and-bla-imp-4
#17
Liu Lu, Yu Feng, Haiyan Long, Alan McNally, Zhiyong Zong
A carbapenem-resistant Klebsiella pneumoniae was recovered from human blood. Its whole genome sequence was obtained using both Illumina and long-read MinION sequencing. The strain belongs to ST273, which has been found recently and caused an outbreak in Southeast Asia. It has two carbapenemase genes bla NDM-1 (carried by an ST7 IncN self-transmissible plasmid) and bla IMP-4 (located on an self-transmissible IncHI5 plasmid). Non-KPC-producing ST237 may represent a lineage of carbapenem-resistant K. pneumoniae , which warrants further monitoring...
April 2, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29610205/relebactam-is-a-potent-inhibitor-of-the-kpc-2-%C3%AE-lactamase-and-restores-the-susceptibility-of-imipenem-against-kpc-producing-enterobacteriaceae
#18
Krisztina M Papp-Wallace, Melissa D Barnes, Jim Alsop, Magdalena A Taracila, Christopher R Bethel, Scott A Becka, David van Duin, Barry N Kreiswirth, Keith S Kaye, Robert A Bonomo
The imipenem-relebactam combination is in development as a potential treatment regimen for infections caused by Enterobacteriaceae possessing complex β-lactamase backgrounds. Relebactam is a β-lactamase inhibitor that possesses the diazabicyclooctane core similar to avibactam, however relebactam's R1 side chain also includes a piperidine ring compared to the carboxyamide of avibactam. Here, we investigated the inactivation of Klebsiella pneumoniae carbapenemase (KPC-2), the most widespread class A carbapenemase, by relebactam and performed susceptibility testing with imipenem-relebactam using KPC-producing clinical isolates of Enterobacteriaceae...
April 2, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29603371/modified-carba-np-test-simple-and-rapid-method-to-differentiate-kpc-and-mbl-producing-klebsiella-species
#19
Nitin Kumar, Varsha A Singh, Vikas Beniwal, Shinu Pottathil
BACKGROUND: The aim of this study was to evaluate the modified Carba NP test to differentiate KPC (Klebsiella pneumoniae carbapenemase)- and MBL (metallo-β-lactamase)-producing Klebsiella species. METHODS: A total of 508 non-duplicate clinical isolates of Klebsiella spp. were processed by modified Carba NP and combined disc tests which were further confirmed by conventional polymerase chain reaction (PCR), a gold standard method for statistical analysis. RESULTS: Modified Carba NP test demonstrated 91...
March 30, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29599157/draft-genome-sequence-of-an-ndm-1-and-kpc-2-coproducing-hypervirulent-carbapenem-resistant-klebsiella-pneumoniae-strain-isolated-from-burn-wound-infections
#20
Dan-Dan Wei, La-Gen Wan, Yang Liu
We report here the draft genome sequence of an NDM-1- and KPC-2-coproducing hypervirulent carbapenem-resistant Klebsiella pneumoniae strain, isolated from a 58-year-old male in the People's Republic of China with a burn injury.
March 29, 2018: Genome Announcements
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