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https://www.readbyqxmd.com/read/27910164/hplc-uv-and-lc-ms-analyses-of-acylquinic-acids-in-geigeria-alata-dc-oliv-hiern-and-their-contribution-to-antioxidant-and-antimicrobial-capacity
#1
Dimitrina Zheleva-Dimitrova, Reneta Gevrenova, Maya M Zaharieva, Hristo Najdenski, Silviya Ruseva, Valentin Lozanov, Vessela Balabanova, Sakina Yagi, Georgi Momekov, Vanio Mitev
INTRODUCTION: Geigeria alata is a traditional plant used in Sudanese folk medicine for treatment of diabetes, cough, epilepsy and intestinal complaints. OBJECTIVE: To analyze phenolic acids in Geigeria alata roots and leaves and to evaluate their antioxidant and antimicrobial activities. METHODOLOGY: Phenolic acids in the aqueous-methanol extracts were identified by LC-MS. Major compounds were isolated using low-pressure liquid chromatography...
December 1, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27907867/adsorption-characteristics-of-adsorbent-resins-and-antioxidant-capacity-for-enrichment-of-phenolics-from-two-phase-olive-waste
#2
Zhihong Wang, Chengzhang Wang, Jiaojiao Yuan, Changwei Zhang
In this study, the adsorption properties of nine resins including polyamide resin (30-60), polyamide resin (60-100) AB-8, S-8, D-101, NKA-9, NKA-II, XDA-1 and XDA-4 for enrichment phenolics of the olive waste were investigated. XDA-1 and NKA-II were chosen for further study due to their outstanding adsorption and desorption capacity. XDA-1 and NKA-II had similar adsorption and desorption behaviors for phenolics of olive waste. The adsorption mechanism could be better explained by pseudo second-order kinetics model and Freundlich isotherm model, and the adsorption processes were spontaneously and exothermic...
November 24, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27905567/physical-characterization-and-antioxidant-activity-of-thymol-solubilized-tween-80-micelles
#3
Ling-Li Deng, Maierhaba Taxipalati, Fei Que, Hui Zhang
Attempts were made to solubilize thymol in Tween 80 micelle to study the solubilization mechanism of thymol and the effect of solubilization on its antioxidant activity. The maximum solubilized concentration of thymol in a 2.0% (w/v) Tween 80 micelle solution is 0.2 wt%. There was no significant difference in Z-average diameter between the empty micelles and thymol solubilized micelles. (1)H NMR spectra indicated that 3-H and 4-H on the benzene ring of thymol interacted with the ester group between the hydrophilic head group and the hydrophobic tail group of Tween 80 by Van der Waals' force...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27904404/phenolic-composition-antioxidant-capacity-and-in-vitro-cytotoxicity-assessment-of-fruit-wines
#4
Ana Ljevar, Natka Ćurko, Marina Tomašević, Kristina Radošević, Višnja Gaurina Srček, Karin Kovačević Ganić
Fruit wines contain a wide range of phenolic compounds with biological effects, but their composition and potential benefits to human health have been studied to the much lesser extent compared to grape wines. The aim of this research is to study the phenolic profile of different types of fruit wines and to evaluate their antioxidant and biological potential. Commercially available fruit wines from blackberry, cherry, raspberry, blackcurrant, strawberry and apple produced in Croatia were analyzed. To the best of our knowledge, this study represents the first comprehensive screening of Croatian fruit wines...
June 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27899047/phytochemicals-and-antioxidant-capacity-in-four-italian-traditional-maize-zea-mays-l-varieties
#5
Antonella Capocchi, Stefania Bottega, Carmelina Spanò, Debora Fontanini
Flours of four pigmented (from orange to red and dark red) local Italian corns, studied for their soluble, soluble conjugate, and insoluble-bound phenols and flavonoids, showed a prevalence of the insoluble-bound fraction (70-80%). Correlations were found between the flours antioxidant capacity, measured with CUPRAC, FRAP, and DPPH methods, and soluble phenols and flavonoids content. A correlation was also found between ascorbic acid content and flours antioxidant power. Anthocyanins were present in small amounts in the red/dark red seeds; however, acid-alcohol assays and spectral analyses of pericarp extracts indicated the presence of red-brick phlobaphenes in these varieties...
November 29, 2016: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/27897089/anti-aging-effect-of-metformin-on-brain-in-naturally-aged-and-accelerated-senescence-model-of-rat
#6
Geetika Garg, Sandeep Singh, Abhishek Kumar Singh, Syed Ibrahim Rizvi
Metformin, a biguanide, is a widely used anti-diabetic drug which inhibits gluconeogenesis and is used to treat hyperglycemia in type 2 diabetes. Through activation of AMPK pathway, metformin also mimics caloric restriction health benefits. Aging causes substantial molecular to morphological changes in brain, the brain cells being more susceptible towards oxidative stress mediated damages due to the presence of high lipid content and higher oxygen consumption. Wistar rats (naturally aged and D Galactose induced rat model) were supplemented with metformin (300 mg/kg b...
November 29, 2016: Rejuvenation Research
https://www.readbyqxmd.com/read/27896020/identification-of-phenolic-secondary-metabolites-from-schotia-brachypetala-sond-fabaceae-and-demonstration-of-their-antioxidant-activities-in-caenorhabditis-elegans
#7
Mansour Sobeh, Esraa ElHawary, Herbenya Peixoto, Rola M Labib, Heba Handoussa, Noha Swilam, Ahmed H El-Khatib, Farukh Sharapov, Tamer Mohamed, Sonja Krstin, Michael W Linscheid, Abdel Nasser Singab, Michael Wink, Nahla Ayoub
BACKGROUND: Schotia brachypetala Sond. (Fabaceae) is an endemic tree of Southern Africa whose phytochemistry and pharmacology were slightly studied. The present work aimed at profiling the major phenolics compounds present in the hydro-alcohol extract from S. brachypetala leaves (SBE) using LC/HRESI/MS/MS and NMR and prove their antioxidant capabilities using novel methods. METHODS: In vitro assays; DPPH, TEAC persulfate decolorizing kinetic and FRAP assays, and in vivo assays: Caenorhabditis elegans strains maintenance, Intracellular ROS in C...
2016: PeerJ
https://www.readbyqxmd.com/read/27895386/addition-of-strawberries-to-the-usual-diet-increases-postprandial-but-not-fasting-non-urate-plasma-antioxidant-activity-in-healthy-subjects
#8
Anna Prymont-Przyminska, Piotr Bialasiewicz, Anna Zwolinska, Agata Sarniak, Anna Wlodarczyk, Jaroslaw Markowski, Krzysztof P Rutkowski, Dariusz Nowak
Strawberries can augment plasma antioxidant activity, but this may be confounded by selection of methods, time of blood sampling and concomitant dietary restrictions. We examined the effect of strawberry consumption on ferric reducing ability (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (DPPH-test) of native and non-urate plasma in healthy subjects on their usual diet. Eleven subjects consumed strawberries (500 g daily) for 9 days. Fasting and 3-h postprandial plasma and 24-h urine collection were obtained before, during and after strawberry course for FRAP, DPPH-test and polyphenols determination...
November 2016: Journal of Clinical Biochemistry and Nutrition
https://www.readbyqxmd.com/read/27891361/serum-frap-levels-and-pre-eclampsia-among-pregnant-women-in-a-rural-community-of-northern-india
#9
Anant Gupta, Shashi Kant, Sanjeev Kumar Gupta, Shyam Prakash, Mani Kalaivani, Chandrakant S Pandav, Sanjay Kumar Rai, Puneet Misra
INTRODUCTION: There is a balance between the oxidative and the anti-oxidative forces in human body. Some studies document decreased level of anti-oxidant in pre-eclampsia while other studies showed normal level of anti-oxidant in pre-eclampsia and the evidence is equivocal. AIM: The aim of the present study was to assess enzymatic anti-oxidant activities in pre-eclamptic women and compare it with normotensive pregnant women with period of gestation between 28 to 36 weeks...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27877914/interaction-of-poly-ethylene-glycol-conjugated-phospholipids-with-supported-lipid-membranes-and-their-influence-on-protein-adsorption
#10
Toshihiro Yamamoto, Yuji Teramura, Toru Itagaki, Yusuke Arima, Hiroo Iwata
We studied real-time interaction between poly(ethylene glycol)-conjugated phospholipids (PEG-lipids) and a supported lipid membrane by surface plasmon resonance (SPR) spectroscopy to understand dynamic behaviors of PEG-lipids on living cell membranes. Supported lipid membranes formed on a hydrophobic surface were employed as a model of living cell membrane. We prepared three kinds of PEG-lipids that carried alkyl chains of different lengths for SPR measurements and also performed fluorescence recovery after photobleaching (FRAP) to study the influence of acyl chain length on dynamics on the supported membrane...
2016: Science and Technology of Advanced Materials
https://www.readbyqxmd.com/read/27876214/beneficial-effects-of-plantago-albicans-on-high-fat-diet-induced-obesity-in-rats
#11
Noura Samout, Amani Ettaya, Hafsia Bouzenna, Sana Ncib, Abdelfattah Elfeki, Najla Hfaiedh
Obesity is a one of the main global public health problems associated with chronic diseases such as coronary heart disease, diabetes and cancer. As a solution to obesity, we suggest Plantago albicans, which is a medicinal plant with several biological effects. This study assesses the possible anti-obesity protective properties of Plantago albicans in high fat diet-fed rats. 28 male Wistar rats were divided into 4 groups; a group which received normal diet (C), the second group was fed HDF diet (HDF), the third group was given normal diet supplemented with Plantago albicans (P...
November 18, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27872932/phytochemical-profiles-of-marine-phytoplanktons-an-evaluation-of-their-in-vitro-antioxidant-and-anti-proliferative-activities
#12
Jim Jun-Hui Huang, Wen-Wen Xu, Shao-Ling Lin, Peter Chi-Keung Cheung
Marine microorganisms such as phytoplanktons are a rich resource of bioactive components with antioxidant and anti-proliferative activities that can act as novel functional food ingredients. In this study, the pigment profiles, total mycosporine-like amino acids (MAAs) and total phenolic contents (TPCs) in solvent extracts including 90% acetone and methanol from five marine phytoplanktons including Nitzschia closterium (Bacillariophyta), Isochrysis zhangjiangensis (Haptophyta), Platymonas subcordiformis (Chlorophyta), Porphyridium cruentum (Rhodophyta) and Synechocystis pevalekii (Cyanobacteria) were analyzed...
November 22, 2016: Food & Function
https://www.readbyqxmd.com/read/27871651/erk-activated-by-histamine-h1-receptor-is-anti-proliferative-through-spatial-restriction-in-the-cytosol
#13
Ruchi Jain, Uchenna Watson, Deepak Kumar Saini
Histamine, a primary mediator of allergic responses, elicits its effects by activating specific receptors belonging to the GPCR family in target cells. Activation of histamine receptor can activate MAP kinases as recorded by monitoring the phosphorylation of extracellular signal regulated kinase (ERK). Despite this, ERK phosphorylation does not translate into pro-proliferative changes after histamine stimulation in HeLa cells. Here we show that histamine H1 receptor activation mediates MAPK activation through PLCβ, Src, PKCδ and MEK pathway, but does not lead to nuclear relocalization of phospho-ERK (pERK), classically associated with pro-proliferative changes...
October 25, 2016: European Journal of Cell Biology
https://www.readbyqxmd.com/read/27869259/the-effect-of-top-layer-chemistry-on-the-formation-of-supported-lipid-bilayers-on-polyelectrolyte-multilayers-primary-versus-quaternary-amines
#14
E Diamanti, P Andreozzi, R Anguiano, L Yate, D Gregurec, N Politakos, R F Ziolo, E Donath, S E Moya
The influence of the surface chemistry of polyelectrolyte multilayers (PEMs) on the formation of lipid bilayers is studied here for PEMs with either polyallylamine hydrochloride (PAH) or polydiallyldimethylammonium chloride (PDADMAC) as a polycation as a top layer, and polystyrene sulfonate (PSS) as a polyanion. Small unilamellar vesicles (SUVs) composed of phosphatidyl choline and phosphatidyl serine at a 50 : 50 molar ratio are deposited on top of the PEM films. The assembly of the SUVs into bilayers is studied via a quartz crystal microbalance with dissipation (QCM-D) and fluorescence recovery after photobleaching (FRAP)...
November 30, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27865618/actinidia-arguta-supplementation-protects-aorta-and-liver-in-rats-with-induced-hypercholesterolemia
#15
Maria Leontowicz, Hanna Leontowicz, Iwona Jesion, Wojciech Bielecki, Katarzyna Najman, Piotr Latocha, Yong-Seo Park, Shela Gorinstein
There are no published results focusing on the study of hardy kiwifruit as a supplementation to the atherogenic diet. We hypothesized that hardy kiwifruit (Actinidia arguta (A. arguta)) from Poland possess better pro-healthy action than two Asian varieties (Hayward and Bidan). We tested this hypothesis by measuring the metabolic reactions of rats loaded with 1% cholesterol and supplemented with 5% of hardy kiwifruit (A. arguta), Hayward, or Bidan in their diets. The experiment was performed on 71 male Wistar rats...
September 30, 2016: Nutrition Research
https://www.readbyqxmd.com/read/27865264/changes-in-serum-biomarkers-of-oxidative-stress-after-treatment-for-canine-leishmaniosis-in-sick-dogs
#16
Camila Peres Rubio, Silvia Martinez-Subiela, Asta Tvarijonaviciute, Josefa Hernández-Ruiz, Luis Pardo-Marin, Sergi Segarra, José Joaquín Ceron
Canine leishmaniosis (CanL) is a zoonotic disease being endemic in several parts of the world. In this study we investigated the behavior of a panel of biomarkers of oxidative stress in 12 sick dogs naturally infected by CanL before and at days 30 and 180 of a successful therapy with a standard treatment. The assays total oxidant status (TOS), trolox equivalent antioxidant capacity (TEAC), ferric reducing ability of plasma (FRAP), cupric reducing antioxidant capacity (CUPRAC), serum thiol and paraoxonase 1 (PON1) were included in the panel...
December 2016: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/27862315/evaluation-of-salivary-nitric-oxide-levels-and-c-reactive-protein-in-patients-with-oral-lichen-planus-and-burning-mouth-syndrome
#17
Tvarijonaviciute Asta, Aznar Cayuela Cristina, Peres Rubio Camila, Ceron José Joaquin, Pia López-Jornet
OBJECTIVES: The aim of this study was to evaluate oxidative stress factors and C-reactive protein in the saliva of patients with oral lichen planus (OLP) and burning mouth syndrome (BMS). METHODS: This consecutive, cross-sectional study included 20 patients with OLP, 19 with burning mouth syndrome (BMS), and 31 control subjects. The oral cavity of each patient was examined and patients responded to a quality of life questionnaire (OHIP-14) and the Xerostomia Inventory...
November 8, 2016: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/27860049/pimpinella-anisum-l-ethanolic-extract-ameliorates-the-gentamicin-induced-nephrotoxicity-in-rats
#18
Saeed Changizi-Ashtiyani, Amirhassan Seddigh, Houshang Najafi, Nasser Hossaini, Amir Avan, Ahmad Akbary, Mostafa Manian, Reza Nedaeinia
AIM: Gentamicin (GM) is one of the commonest causes of drug-induced nephrotoxicity. Moreover, oxidative stress plays an important role in gentamicin-induced nephrotoxicity. The current study aimed to explore the anti-oxidant and protective effects of Pimpinella anisum (P. anisum) on the alleviation of GM-induced damage. METHODS: Forty male wistar rats were divided into four groups: control, sham that was administrated normal saline orally and intraperitoneally (i...
November 8, 2016: Nephrology
https://www.readbyqxmd.com/read/27855892/cooked-garlic-and-antioxidant-activity-correlation-with-organosulfur-compound-composition
#19
D A Locatelli, M A Nazareno, C M Fusari, A B Camargo
The antioxidant properties and the main beneficial organosulphur compounds of home-cooked garlic samples were studied in order to establish relationships between them. Antioxidant activity was tested by free radical scavenging against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2'-azino-bis-(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS(+)), Fe(III) reducing ability (FRAP) and linoleic acid co-oxidation initiated by soybean lipoxygenase in a micelle system. DPPH, ABTS and FRAP assays showed the highest activity for raw garlic samples, while β-carotene bleaching assay yielded the highest activity for stir-fried garlic...
January 0: Food Chemistry
https://www.readbyqxmd.com/read/27853107/mechanisms-underlying-the-toxic-effects-of-antimony-species-in-human-embryonic-kidney-cells-hek-293-and-their-comparison-with-arsenic-species
#20
Marcelo Verdugo, Yasumitsu Ogra, Waldo Quiroz
Antimony cytotoxicity was assessed in human embryonic kidney cells (HEK-293). Uptake, mitochondrial respiratory activity, ROS generation and diffusional kinetics were measured using fluorescence recovery after photobleaching (FRAP). Furthermore, the toxic effect induced by Sb was compared with As toxicity in regard to ROS generation and diffusional kinetics, which provides information on the protein aggregation process. Our results show a favored uptake of Sb(III) and a more severe effect, decreasing the mitochondrial activity more than in the presence of Sb(V)...
2016: Journal of Toxicological Sciences
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