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https://www.readbyqxmd.com/read/28447802/potential-lipid-lowering-mechanisms-of-biochanin-a
#1
Zhaohui Xue, Qian Zhang, Wancong Yu, Haichao Wen, Xiaonan Hou, Dan Li, Xiaohong Kou
Extensive studies have demonstrated that biochanin A (BCA) has a significant hypolipidemic effect. However, its mechanism of action is not clear. In this context, the effect of BCA on a high-fat diet (HFD)-induced hyperlipidemia in mice was determined. The results showed that treatment with a medium dose of biochanin A (BM) significantly decreased low density lipoprotein cholesterol (LDL-C) 85% (from 1.196 ± 0.183 to 0.181 ± 0.0778 mM) and total cholesterol (TC) 39% (from 5.983 ± 0.128 to 3.649 ± 0.374 mM) levels, increased lipoprotein lipase (LPL) 96% (from 1...
April 27, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28447512/centella-asiatica-enhances-hepatic-antioxidant-status-and-regulates-hepatic-inflammatory-cytokines-in-type-2-diabetic-rats
#2
Ayodeji B Oyenihi, Novel N Chegou, Oluwafemi O Oguntibeju, Bubuya Masola
CONTEXT: Neutralizing the over-activation of oxidative stress and inflammation remains an important goal in the management of type 2 diabetes mellitus (T2DM). Centella asiatica (L.) Urban (Apiaceae) (CA) has been used in traditional folklore in Africa and Asia to treat various ailments including diabetes. OBJECTIVE: We investigated the hepatic antioxidant and anti-inflammatory potential of methanol extract of CA leaves in T2DM. MATERIALS AND METHODS: T2DM was induced in male Sprague-Dawley rats with 10% fructose in drinking water for 14 days followed by a single intraperitoneal injection of streptozotocin (40 mg/kg b...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28447361/the-effect-of-dietary-chlorella-vulgaris-inclusion-on-goat-s-milk-chemical-composition-fatty-acids-profile-and-enzymes-activities-related-to-oxidation
#3
E Tsiplakou, M A M Abdullah, A Mavrommatis, M Chatzikonstantinou, D Skliros, K Sotirakoglou, E Flemetakis, N E Labrou, G Zervas
The impact of dietary supplementation with microalgae on goat's milk chemical composition, fatty acids (FA) profile and enzymes activities related to antioxidant mechanism has not been well documented. Thus, this study aimed to investigate the effects of dietary inclusion of Chlorella vulgaris on the following: (i) milk yield, chemical composition and FA profile, (ii) the activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione transferase (GST) and glutathione peroxidase (GSH-Px) in blood plasma and (iii) the activities of SOD, GR and lactoperoxidase (LPO) in milk of goats...
April 26, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28447344/short-term-treatments-with-high-co2-and-low-o2-concentrations-on-quality-of-fresh-goji-berries-lycium-barbarum-l-during-cold-storage
#4
Mina Kafkaletou, Miltiadis V Christopoulos, Eleni Tsantili
BACKGROUND: Goji berries (Lycium barbarum L.) are functional fruits, but usually disposed to markets as dried. The aim, here, was to investigate the storability of fresh goji berries treated with high CO2 and low O2 concentrations before air storage at 1 °C for 21 d. RESULTS: Berries harvested without stems were exposed to air (controls) or treated for 2 d at 1 °C with the following controlled atmosphere (CA) treatments: 21% O2 + 0% CO2 (21 + 0), 5% O2 + 15% CO2 (5 + 15), 10% O2 + 10% CO2 (10 + 10) and 20% O2 + 20% CO2 (20 + 20)...
April 26, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28447255/phenolic-compounds-volatiles-and-antioxidant-capacity-of-white-myrtle-berry-liqueurs
#5
Gabriele Serreli, Igor Jerković, Katarzyna Angelika Gil, Zvonimir Marijanović, Viviana Pacini, Carlo Ignazio Giovanni Tuberoso
The aim of this research was to evaluate the antioxidant capacity and physical-chemical characteristics of commercial white myrtle berry (Myrtus communis L. var. leucocarpa DC) liqueur (WMBL). The total phenolic (TP) content was measured spectrophotometrically, applying a modified Folin-Ciocalteu's method, and phenolic compounds were identified by high-performance liquid chromatography (HPLC) coupled with electrospray mass spectrometry, and quantified by HPLC coupled with ultraviolet/visible detection. The antioxidant capacities were evaluated by FRAP, CUPRAC, DPPH(•), and ABTS(•+) assays...
April 26, 2017: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/28445762/analysis-of-active-transport-by-fluorescence-recovery-after-photobleaching
#6
Maria-Veronica Ciocanel, Jill A Kreiling, James A Gagnon, Kimberly L Mowry, Björn Sandstede
Fluorescence recovery after photobleaching (FRAP) is a well-established experimental technique to study binding and diffusion of molecules in cells. Although a large number of analytical and numerical models have been developed to extract binding and diffusion rates from FRAP recovery curves, active transport of molecules is typically not included in the existing models that are used to estimate these rates. Here we present a validated numerical method for estimating diffusion, binding/unbinding rates, and active transport velocities using FRAP data that captures intracellular dynamics through partial differential equation models...
April 25, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28427041/the-efficacy-of-donepezil-administration-on-acetylcholinesterase-activity-and-altered-redox-homeostasis-in-alzheimer-s-disease
#7
Pinar Atukeren, Mahir Cengiz, Hakan Yavuzer, Remise Gelisgen, Esma Altunoglu, Sena Oner, Fusun Erdenen, Damla Yuceakın, Himmet Derici, Ufuk Cakatay, Hafize Uzun
Alzheimer's disease (AD) is a serious multifactorial disorder with progressive neurodegenerative outcomes related with impaired redox homeostasis. Inhibition of the enzyme acetylcholinesterase (AChE), as one of the major therapeutic strategies, is considered to be offering only symptomatic relief and moderate disease modifying effect. We intended to investigate the effects of acetylcholinesterase inhibition via donepezil on protein carbonyl (PCO), advanced protein oxidation products (AOPP) and ischemia modified albumin (IMA) as protein oxidation markers and ferric reducing antioxidant power (FRAP), prooxidant-antioxidant balance (PAB), total thiol (T-SH), protein thiol (P-SH) as antioxidant status markers and also kynurenine (KYN), N-formyl kynurenine (N-FKYN) and protein bound dityrosine (DT) levels all in one demonstrating the redox homeostasis in Alzheimer patients also correlated with AChE activity...
April 17, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28424264/identification-of-a-multienzyme-complex-for-glucose-metabolism-in-living-cells
#8
Casey L Kohnhorst, Minjoung Kyoung, Miji Jeon, Danielle L Schmitt, Erin L Kennedy, Julio Ramirez, Syrena M Bracey, Bao Tran Luu, Sarah J Russell, Songon An
Sequential metabolic enzymes in glucose metabolism have long been hypothesized to form multienzyme complexes that regulate glucose flux in living cells. However, it has been challenging to directly observe these complexes and their functional roles in living systems. In this work, we have used wide-field and confocal fluorescence microscopy to investigate the spatial organization of metabolic enzymes participating in glucose metabolism in human cells. We provide compelling evidence that human liver-type phosphofructokinase 1 (PFKL), which catalyzes a bottleneck step of glycolysis, forms various sizes of cytoplasmic clusters in human cancer cells, independent of protein expression levels and of the choice of fluorescent tags...
April 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28424022/analytical-validation-of-an-automated-assay-for-ferric-reducing-ability-of-plasma-in-dog-serum
#9
Camila Peres Rubio, Silvia Martinez-Subiela, Josefa Hernández-Ruiz, Asta Tvarijonaviciute, José Joaquín Ceron
We performed analytical validation of an automated ferric-reducing ability of plasma (FRAP) assay in the serum of dogs. Intra- and interassay precision, accuracy, detection limit, and effects of hemolysis and lipemia were evaluated. Intra- and interassay coefficients of variation were <1% and <13%, respectively. The assay showed a high correlation with a FRAP assay described previously, and results were linear when serial sample dilutions were tested. The detection limit was lower than the values observed in sera from healthy dogs; decreased serum FRAP was found in dogs with leishmaniosis...
April 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28417557/a-multitarget-approach-toward-the-development-of-a-new-series-of-8-substituted-purines-for-photoprotection-and-prevention-of-ultra-violet-uv-related-damages
#10
Ernestine Nicaise Djuidje, Valeria Dissette, Alessia Bino, Simonetta Benetti, Jan Balzarini, Sandra Liekens, Stefano Manfredini, Silvia Vertuani, Anna Baldisserotto
In environmental abundance, UV is the most important modifiable risk factor for skin cancer and many other skin diseases such as early photo-aging. Among solar radiation, ultraviolet (UV) is mainly responsible for inducing skin problems. In search of novel photoprotective purines, a new series of 8-substituted purines were synthesized from commercially available 6-hydroxy-4,5-diaminopyrimidine hemisulfate or 4,5-diaminopyrimidine. All the title compounds were investigated for their UV-filter, antioxidant, antifugal and antiproliferative activities...
April 18, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28417368/bioinspired-assemblies-of-plant-cell-walls-for-measuring-protein-carbohydrate-interactions-by-frap
#11
Gabriel Paës
The interactions of proteins involved in plant cell wall hydrolysis, such as enzymes and CBMs, significantly determine their role and efficiency. In order to go beyond the characterization of interactions with simple ligands, bioinspired assemblies combined with the measurement of diffusion and interaction by FRAP offer a relevant alternative for highlighting the importance of different parameters related to the protein affinity and to the assembly.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28416338/separation-and-purification-of-polyphenols-from-red-wine-extracts-using-high-speed-counter-current-chromatography
#12
Yuanyuan Li, Lingxi Li, Yan Cui, Shuting Zhang, Baoshan Sun
Polyphenols are important compounds of red wine owing to their contribution to sensory properties and antioxidant activities. In this study, high-speed counter-current chromatography (HSCCC) coupled with semi-preparative HPLC was used for large-scale separation and purification of polyphenols from red wine extracts. With the solvent system of hexane-ethyl acetate-water (1-50-50), various oligomeric procyanidins including monomer catechin, epicatechin, dimers B1, B2; phenolic acids including coutaric acid, caftaric acid and other type of polyphenols were largely separated within 370min and most of these compounds presented high yields (0...
March 12, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28408867/visualization-of-snare-mediated-hemifusion-between-giant-unilamellar-vesicles-arrested-by-myricetin
#13
Paul Heo, Joon-Bum Park, Yeon-Kyun Shin, Dae-Hyuk Kweon
Neurotransmitters are released within a millisecond after Ca(2+) arrives at an active zone. However, the vesicle fusion pathway underlying this synchronous release is yet to be understood. At the center of controversy is whether hemifusion, in which outer leaflets are merged while inner leaflets are still separated, is an on-pathway or off-pathway product of Ca(2+)-triggered exocytosis. Using the single vesicle fusion assay, we recently demonstrated that hemifusion is an on-pathway intermediate that immediately proceeds to full fusion upon Ca(2+) triggering...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28407963/an-improved-method-for-extraction-of-nutraceutically-important-polyphenolics-from-berberis-jaeschkeana-c-k-schneid-fruits
#14
Tarun Belwal, Lalit Giri, Indra D Bhatt, Ranbeer S Rawal, Veena Pande
Berberis jaeschkeana fruits, source of nutraceutically important polyphenolics were investigated. A total of 32 experimental run were conducted under Plackett-Burman and central composite design. Microwave power, methanol and HCl concentration significantly (p<0.05) affect extraction of polyphenols under linear, quadratic and interactive effect. The model showed good fitness with significant (p<0.05) model F-value and a non-significant lack of fit. Under optimum microwave assisted extraction (MAE) condition the total phenolics, flavonoids, tannins and antioxidant activity were in closed context with predicted values...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28407953/antioxidant-potential-of-edible-mushroom-agaricus-bisporus-protein-hydrolysates-and-their-ultrafiltration-fractions
#15
Benard Muinde Kimatu, Liyan Zhao, Yuan Biao, Gaoxing Ma, Wenjian Yang, Fei Pei, Qiuhui Hu
Mushroom protein isolate (MPI) from Agaricus bisporus was hydrolyzed using Alcalase, Pancreatin, Flavourzyme, Alcalase-Pancreatin and Alcalase-Flavourzyme. The obtained hydrolysates (MPHs) were ultrafiltered to generate peptide fractions (UFs) of molecular sizes (<1, 1-3, 3-5 and 5-10kDa). The electrophoretic profile results indicated that the enzymatic systems were efficient in hydrolyzing the MPI into low molecular weight peptides. Hydrolysate yields of >57% and protein recoveries of >43% were obtained...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28404222/the-effect-of-different-extraction-techniques-on-property-and-bioactivity-of-polysaccharides-from-dioscorea-hemsleyi
#16
Chengcheng Zhao, Xia Li, Jing Miao, Songsong Jing, Xuejiao Li, Luqi Huang, Wenyuan Gao
The rhizoma of Dioscorea hemsleyi (DH) has been used as a treatment of diabetes in China for hundreds of years. Polysaccharides in DH were extracted by using ultrasonic-assisted extraction (UAE), cold water extraction (CWE), warm water extraction (WWE) and hot water extraction (HWE), separately. Then the different characterizations of four DH polysaccharide (DHP) samples were analyzed by high-performance liquid chromatography (HPLC), high-performance Gel permeation chromatography (HGPC), ultraviolet-visible spectroscopy(UV), fourier transform-infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM)...
April 9, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28400325/juncaceae-species-as-sources-of-innovative-bioactive-compounds-for-the-food-industry-in%C3%A2-vitro-antioxidant-activity-neuroprotective-properties-and-in-silico-studies
#17
Maria João Rodrigues, Katkam N Gangadhar, Gokhan Zengin, Adriano Mollica, João Varela, Luísa Barreira, Luísa Custódio
Several Juncus species are traditionally used as sedative and to treat health problems like insomnia. This work was based on the hypothesis that Juncus acutus, J. maritimus and J. inflexus may have molecules with bioactivities relevant for the improvement of cognitive functions and thus with potential use as food additives and/or nutraceuticals. Therefore leaves and roots extracts of those species were evaluated for radical scavenging (RSA) and metal chelating activities, and for in vitro inhibition of acetyl-(AChE) and butyrylcholinesterase (BuChE)...
April 8, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28400224/new-amides-from-seeds-of-silybum-marianum-with-potential-antioxidant-and-antidiabetic-activities
#18
Ning-Bo Qin, Cui-Cui Jia, Jun Xu, Da-Hong Li, Fan-Xing Xu, Jiao Bai, Zhan-Lin Li, Hui-Ming Hua
Two new amide compounds, mariamides A and B (1-2), were obtained together with fourteen known compounds from the seeds of milk thistle (Silybum marianum). Their structures were established on the basis of extensive 1D and 2D NMR analyses, as well as HR-ESI-MS data. Most of the compounds showed significant antioxidant activities than positive control in ABTS and FRAP assays. However, only amide compounds 1-4 showed moderate DPPH radical scavenging activity and compounds 7 and 16 showed the most potent activity against DPPH...
April 8, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28393917/inhibition-of-melanogenesis-by-jineol-from-scolopendra-subspinipes-mutilans-via-map-kinase-mediated-mitf-downregulation-and-the-proteasomal-degradation-of-tyrosinase
#19
Md Badrul Alam, Vivek K Bajpai, JungIn Lee, Peijun Zhao, Jung-Hee Byeon, Jeong-Sic Ra, Rajib Majumder, Jong Sung Lee, Jung-In Yoon, Irfan A Rather, Yong-Ha Park, Kangmin Kim, MinKyun Na, Sang-Han Lee
In this study, the authors investigated the anti-melanogenic effects of 3,8-dihydroxyquinoline (jineol) isolated from Scolopendra subspinipes mutilans, the mechanisms responsible for its inhibition of melanogenesis in melan-a cells, and its antioxidant efficacy. Mushroom tyrosinase activities and melanin contents were determined in melan-a cells, and the protein and mRNA levels of MITF, tyrosinase, TYRP-1, and TYRP-2 were assessed. Jineol exhibited significant, concentration-dependent antioxidant effects as determined by DPPH, ABTS, CUPRAC, and FRAP assays...
April 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28391725/identification-and-in-vitro-antioxidant-activities-of-phenolic-compounds-isolated-from-cynoglossum-cheirifolium-l
#20
Bensaid Ilhem, Atik Bekkara Fawzia, El Haci Imad Abdelhamid, Belarbi Karima, Beddou Fawzia, Bekhechi Chahrazed
In an extensive search for bioactive compounds from plant sources, the quantitative and qualitative characterisation of the compounds present in Cynoglossum cheirifolium extracts was studied. Total phenolic and flavonoid contents were determined by spectrophotometric techniques. In vitro antioxidant and radical scavenging profiling was determined through DPPH(•) scavenging activity and Ferric reducing antioxidant power (FRAP). Our study showed that leaves produce more phenolic compounds, followed by flowering aerial part...
April 9, 2017: Natural Product Research
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