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https://www.readbyqxmd.com/read/28343034/bioactivities-of-achillea-phrygia-and-bupleurum-croceum-based-on-the-composition-of-phenolic-compounds-in-vitro-and-in-silico-approaches
#1
Gokhan Zengin, Gizem Bulut, Adriano Mollica, Mehmet Zeki Haznedaroglu, Ahmet Dogan, Abdurrahman Aktumsek
This study presents the effects of the Achillea phrygia Boiss. et Bal. and Bupleurum croceum Fenzl. extracts obtained by different solvents (ethyl acetate, methanol and water) on selected enzyme inhibitory effects and antioxidant ability with screening bioactive compounds. Total and individual bioactive compounds were detected by spectrophotometric and HPLC-DAD techniques. Antioxidant abilities were evaluated by different methods including free radical scavenging (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating...
March 22, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28332429/black-tea-supplementation-augments-redox-balance-in-rats-relevance-to-aging
#2
Dileep Kumar, Syed Ibrahim Rizvi
Several health beneficial effects have been attributed to tea consumption, most of the effects are due to the strong antioxidant property of tea catechins. The present study evaluates the effect of black tea extract (BTE) supplementation on the redox balance of rats at different stages in their life span. We have evaluated erythrocyte and plasma redox status in young (4 months), middle-aged (12 months) and old-aged (24 months) male Wistar rats, by quantifying an array of parameters linked to redox status...
March 23, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28330179/antioxidant-and-antiplatelet-activities-of-flavonoid-rich-fractions-of-three-citrus-fruits-from-korea
#3
Awraris Derbie Assefa, Eun Young Ko, So Hyun Moon, Young-Soo Keum
Three different fractional (methanol, ethyl acetate and hexane) extracts from yuzu (Citrus junos Sieb ex Tanaka), hallabong [(C. unshiu Marcov × C. sinensis Osbeck) × C. reticulata Blanco] and orange (C. sinensis) were evaluated for their antioxidant activity and antiplatelet effects. 2,2-Diphenyl-1-Picrylhydrazyl (DPPH), cupric reducing antioxidant capacity (CUPRAC) and ferric-reducing antioxidant power (FRAP) methods were used for the antioxidant activity tests. Total flavonoids and total phenolics were also evaluated spectrophotometrically...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329408/effects-of-spray-drying-parameters-on-in-vitro-functional-properties-of-camu-camu-myrciaria-dubia-mc-vaugh-a-typical-amazonian-fruit
#4
Alice Fujita, Volnei Brito Souza, Luis Daniel Daza, Carmen Silvia Fávaro-Trindade, Daniel Granato, Maria Inés Genovese
Camu-camu (Myrciaria dubia) fruit is a rich source of bioactive compounds but its shelf life is rather short. Therefore, this study was aimed to evaluate the effect of inlet air temperature (T) and concentration (C) of maltodextrin and arabic gum on the spray-drying process of commercial camu-camu pulps (São Paulo and Manaus). Moisture, solubility, total phenolics (TP), ascorbic acid (AA), and proanthocyanidins (PAC) contents, and in vitro antioxidant capacity of the powders (FRAP, DPPH, Folin-Ciocalteu's reducing capacity were measured)...
March 22, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28327520/cytotoxicity-bactericidal-and-antioxidant-activity-of-sodium-alginate-hydrosols-treated-with-direct-electric-current
#5
Żaneta Król, Krzysztof Marycz, Dominika Kulig, Monika Marędziak, Andrzej Jarmoluk
The aim of the study was to investigate the effect of using direct electric current (DC) of 0, 200, and 400 mA for five minutes on the physiochemical properties, cytotoxicity, antibacterial, and antioxidant activity of sodium alginate hydrosols with different sodium chloride concentrations. The pH, oxidation-reduction potential (ORP), electrical conductivity (EC), and available chlorine concentration (ACC) were measured. The effect of sodium alginate hydrosols treated with DC on Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, Micrococcus luteus, Escherichia coli, Salmonella enteritidis, Yersinia enterocolitica, Pseudomonas fluorescence, and RAW 264...
March 22, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28324613/analysis-of-protein-kinetics-using-fluorescence-recovery-after-photobleaching-frap
#6
Nickolaos Nikiforos Giakoumakis, Maria Anna Rapsomaniki, Zoi Lygerou
Fluorescence recovery after photobleaching (FRAP) is a cutting-edge live-cell functional imaging technique that enables the exploration of protein dynamics in individual cells and thus permits the elucidation of protein mobility, function, and interactions at a single-cell level. During a typical FRAP experiment, fluorescent molecules in a defined region of interest within the cell are bleached by a short and powerful laser pulse, while the recovery of the fluorescence in the region is monitored over time by time-lapse microscopy...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28318946/easy-and-rapid-preparation-of-benzoylhydrazides-and-their-diazene-derivatives-as-inhibitors-of-15-lipoxygenase
#7
Cristian Tirapegui, Williams Acevedo-Fuentes, Pablo Dahech, Claudia Torrent, Pablo Barrias, Macarena Rojas-Poblete, Carolina Mascayano
Two series of diaza derivatives were prepared by solvent-free condensation of benzoic acid and 4-substituted phenylhydrazines in order to obtain phenylhydrazides (HYD series) and, by oxidation of these compounds, the corresponding benzoyldiazenes (DIA series). Both sets were evaluated as inhibitors of soybean 15-lipoxygenase activity and antioxidant capability in the FRAP and CUPRAC assays. The most potent inhibitors of both series exhibited IC50 values in the low micromolar range. Kinetic studies showed that at least the more active compounds were competitive inhibitors...
March 8, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28317769/protective-ability-against-oxidative-stress-of-brewers-spent-grain-protein-hydrolysates
#8
Elsa F Vieira, Diana Dias da Silva, Helena Carmo, Isabel M P L V O Ferreira
The protein fraction of Brewers' spent grain (BSG) was used as substrate to obtain hydrolysates with antioxidant activity. Three enzymatic approaches were applied: brewer's spent yeast (BSY) proteases, Neutrase® and Alcalase®, at the same proteolytic activity (1U/mL), using an enzyme/substrate ratio of 10:100 (v/v), at 50°C, 4h. Total Phenolic Content (TPC) and Ferric Ion Reducing Antioxidant Power (FRAP) of hydrolysates and fractions <10kDa and <3kDa were assayed. Additionally, the protective ability of <10kDa fractions against oxidative stress on Caco-2 and HepG2 cells was investigated...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317748/bioaccessibility-of-bioactive-compounds-and-antioxidant-potential-of-ju%C3%A3-ara-fruits-euterpe-edulis-martius-subjected-to-in-vitro-gastrointestinal-digestion
#9
Mayara Schulz, Fabíola Carina Biluca, Luciano Valdemiro Gonzaga, Graciele da Silva Campelo Borges, Luciano Vitali, Gustavo Amadeu Micke, Jefferson Santos de Gois, Tarcisio Silva de Almeida, Daniel Lazaro Gallindo Borges, Paul Richard Momsen Miller, Ana Carolina Oliveira Costa, Roseane Fett
An in vitro method involving simulated gastrointestinal digestion was used to assess the bioaccessibility of fifteen minerals, twenty-two phenolic compounds and the antioxidant capacity in juçara fruit during seven ripening stages. For minerals and phenolics, respectively, initial contents were up to 1325.9 and 22.9mg100g(-1), whereas after in vitro digestion, the maximum values were 556.7 and 14.43mg100g(-1) (dry matter). Antioxidant capacity, determined by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH) and ferric reducing antioxidant power (FRAP), after in vitro digestion decreased 51-78% when compared to the crude extract...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317705/comparison-of-old-cherry-cultivars-grown-in-czech-republic-by-chemical-composition-and-bioactive-compounds
#10
Agnieszka Nawirska-Olszańska, Joanna Kolniak-Ostek, Maciej Oziembłowski, Alena Ticha, Radomir Hyšpler, Zdenek Zadak, Pavla Židová, Frantisek Paprstein
Sweet cherry fruits are readily consumed by humans, and they contain significant quantities of bioactive compounds. Fifteen sweet cherry (Prunus avium L.) old cultivars grown in the Czech Republic were examined. In fresh fruits the contents of sugars, organic acids, and phenolic compounds, and antioxidant capacity were determined. The analyses showed that the fruits of sweet cherry are characterized by high contents of bioactive compounds. Four organic acids - malic, quinic, shikimic, and fumaric acid - were determined in examined fruits...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28315450/phospholipid-oxidation-and-carotenoid-supplementation-in-alzheimer-s-disease-patients
#11
O S Ademowo, H K I Dias, I Milic, A Devitt, R Moran, R Mulcahy, A N Howard, J M Nolan, H R Griffiths
Alzheimer's disease (AD) is a progressive, neurodegenerative disease, characterised by decline of memory, cognitive function and changes in behaviour. Generic markers of lipid peroxidation are increased in AD and reactive oxygen species have been suggested to be involved in the aetiology of cognitive decline. Carotenoids are depleted in AD serum, therefore we have compared serum lipid oxidation between AD and age-matched control subjects before and after carotenoid supplementation. The novel oxidised phospholipid biomarker 1-palmitoyl-2-(5'-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC) was analysed using electrospray ionisation tandem mass spectrometry (MS) with multiple reaction monitoring (MRM), 8-isoprostane (IsoP) was measured by ELISA and ferric reducing antioxidant potential (FRAP) was measured by a colorimetric assay...
March 14, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28302906/distinct-focal-adhesion-protein-modules-control-different-aspects-of-mechanotransduction
#12
Ben Stutchbury, Paul Atherton, Ricky Tsang, De-Yao Wang, Christoph Ballestrem
Focal adhesions (FAs) are macromolecular complexes that regulate cell adhesion and mechanotransduction. Using fluorescence recovery after photobleaching (FRAP) and fluorescence loss after photoactivation (FLAP), we found that the mobility of core FA proteins correlates with protein function. Structural proteins such as tensin, talin and vinculin are significantly less mobile in FAs than signaling proteins such as FAK and paxillin. The mobilities of the structural proteins are directly influenced by substrate stiffness, suggesting they are involved in sensing the rigidity of the extracellular environment...
March 16, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28301958/unlocking-the-in-vitro-anti-inflammatory-and-antidiabetic-potential-of-polygonum-maritimum
#13
Maria João Rodrigues, Luísa Custódio, Andreia Lopes, Marta Oliveira, Nuno R Neng, José M F Nogueira, Alice Martins, Amélia P Rauter, João Varela, Luísa Barreira
CONTEXT: Several Polygonum species (Polygonaceae) are used in traditional medicine in Asia, Europe and Africa to treat inflammation and diabetes. OBJECTIVE: Evaluate the in vitro antioxidant, anti-inflammatory and antidiabetic potential of methanol and dichloromethane extracts of leaves and roots of the halophyte Polygonum maritimum L. MATERIAL AND METHODS: Antioxidant activity was determined (up to 1 mg/mL) as radical-scavenging activity (RSA) of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), copper (CCA) and iron (ICA) chelating activities and iron reducing power (FRAP)...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28292674/the-anti-oxidant-effects-of-melatonin-derivatives-on-human-gingival-fibroblasts
#14
Chawapon Phiphatwatcharaded, Ploenthip Puthongking, Ponlatham Chaiyarit, Nutjaree Pratheepawanit Johns, Sumon Sakolchai, Pramote Mahakunakorn
OBJECTIVES: Aim of this in vitro study was to evaluate the anti-oxidant activity of indole ring modified melatonin derivatives as compared with melatonin in primary human gingival fibroblast (HGF) cells. METHODS: Anti-oxidant activity of melatonin (MLT), acetyl-melatonin (AMLT) and benzoyl-melatonin (BMLT) was evaluated by5 standard methods as follows: 2, 2-diphenyl-1-picrylhydrazyl (DPPH); ferric ion reducing antioxidant power (FRAP); superoxide anion scavenging; nitric oxide (NO) scavenging; and thiobarbituric acid reactive substances (TBARs)...
March 7, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28289386/traditionally-used-lathyrus-species-phytochemical-composition-antioxidant-activity-enzyme-inhibitory-properties-cytotoxic-effects-and-in-silico-studies-of-l-czeczottianus-and-l-nissolia
#15
Eulogio J Llorent-Martínez, Gokhan Zengin, María L Fernández-de Córdova, Onur Bender, Arzu Atalay, Ramazan Ceylan, Adriano Mollica, Andrei Mocan, Sengul Uysal, Gokalp O Guler, Abdurrahman Aktumsek
Members of the genus Lathyrus are used as food and as traditional medicines. In order to find new sources of biologically-active compounds, chemical and biological profiles of two Lathyrus species (L. czeczottianus and L. nissolia) were investigated. Chemical profiles were evaluated by HPLC-ESI-MS(n), as well as by their total phenolic and flavonoid contents. In addition, antioxidant, enzyme inhibitory, and cytotoxic effects were also investigated. Antioxidant properties were tested by using different assays (DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelation)...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28286807/protein-synthesis-rate-assessment-by-fluorescence-recovery-after-photobleaching-frap
#16
Nikos Kourtis, Nektarios Tavernarakis
Currently available biochemical methods cannot be applied to monitor protein synthesis in specific cells or tissues, in live specimens. Here, we describe a non-invasive method for monitoring protein synthesis in single cells or tissues with intrinsically different translation rates, in live Caenorhabditis elegans animals.
March 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28284335/oxidative-modifications-of-blood-serum-proteins-in-myasthenia-gravis
#17
Monika Adamczyk-Sowa, Edyta Bieszczad-Bedrejczuk, Sabina Galiniak, Izabela Rozmiłowska, Damian Czyżewski, Grzegorz Bartosz, Izabela Sadowska-Bartosz
Myasthenia gravis (MG) is an autoimmune disease caused by production of antibodies against acetylcholine receptors of the neuromuscular junction (Ab). The aim of this study was to ascertain if oxidative stress accompanies MG by estimation of the several independent parameters of oxidative damage, mainly the levels of oxidative modifications of blood serum proteins. The group studied consisted of 50 MG patients (28 females and 22 males), 24 with ocular MG (OMG) and 26 with generalized MG (GMG), of mean age of 66...
April 15, 2017: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/28283081/serum-biomarkers-of-oxidative-stress-in-dogs-with-idiopathic-inflammatory-bowel-disease
#18
C P Rubio, S Martínez-Subiela, J Hernández-Ruiz, A Tvarijonaviciute, J J Cerón, K Allenspach
The objective of this work was to study and compare a panel of various serum biomarkers evaluating both the antioxidant response and oxidative damage in dogs with idiopathic inflammatory bowel disease (IBD). Eighteen dogs with IBD and 20 healthy dogs were enrolled in the study. Trolox equivalent antioxidant capacity (TEAC), cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of the plasma (FRAP), total thiol concentrations, and paraoxonase 1 (PON1) activity were evaluated in serum to determine antioxidant response...
March 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28278638/phytochemical-study-and-biological-effects-of-the-essential-oil-of-fraxinus-dimorpha-coss-durieu-%C3%A2
#19
Soufiane M'sou, Mohamed Alifriqui, Abderrahmane Romane
The present study describes the phytochemical study, antioxidant and antimicrobial activities of Fraxinus dimorpha essential oil, obtained from the leaves collected in the High Atlas Mountains. Forty compounds representing 86.2% with (E)-nerolidol as a major constituent and 29 compounds corresponding to 80.3% with eugenol as main components were identified from Ait M'hamed and from Siti Fadma, respectively. The F. dimorpha essential oil presents IC50 using DPPH varying from 5.33 to 6.03 mg/mL and EC50 using FRAP varying from 0...
February 28, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28274131/antioxidant-phenolic-and-antifungal-profiles-of-acanthus-mollis-acanthaceae
#20
Carlos Jara, Miguel Leyton, Mauricio Osorio, Viviana Silva, Francisco Fleming, Marilyn Paz, Alejandro Madrid, Marco Mellado
Acanthus mollis is used as ornamental and medicinal plant. The ethnopharmacology reports indicate that extracts have anti-inflammatory activity. Phytoconstituents profile was evaluated by estimating the content of anthraquinones, flavonoids and phenols. In addition, the antioxidant activity was evaluated using four methods: Hydrogen atoms transfer (TRAP, ORAC and DPPH assays), and single electron transfer (FRAP assay). Finally, antifungal activity was determined by the M27-A2 test. The results shown that ethanol extracts have the highest concentration of phenols, anthraquinones and flavonoids...
March 9, 2017: Natural Product Research
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