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https://www.readbyqxmd.com/read/29149812/architectures-and-mechanical-properties-of-drugs-and-complexes-of-surface-active-compounds-at-air-water-and-oil-water-interfaces
#1
Dipak K Sarker
BACKGROUND: Drugs can represents a multitude of compounds from proteins and peptides, such as growth hormones and insulin and on to simple organic molecules such as flurbiprofen, ibuprofen and lidocaine. Given the chemical nature of these compounds two features are always present. A portion or portions of the molecule that has little affinity for apolar surfaces and media and the opposite a series of part or one large part that has considerable affinity for hydrophilic, polar or charged media and surfaces...
November 17, 2017: Current Drug Discovery Technologies
https://www.readbyqxmd.com/read/29148062/flagellar-incorporation-of-proteins-follows-at-least-two-different-routes-in-trypanosomes
#2
Laetitia Vincensini, Thierry Blisnick, Eloïse Bertiaux, Sebastian Hutchinson, Christina Georgikou, Cher-Pheng Ooi, Philippe Bastin
BACKGROUND INFORMATION: Eukaryotic cilia and flagella are sophisticated organelles composed of several hundreds of proteins that need to be incorporated at the right time and the right place during assembly. RESULTS: Two methods were used to investigate this process in the model protist Trypanosoma brucei: inducible expression of epitope-tagged labelled proteins and fluorescence recovery after photobleaching (FRAP) of fluorescent fusion proteins. This revealed that skeletal components of the radial spokes (RSP3), the central pair (PF16) and the outer dynein arms (DNAI1) are incorporated at the distal end of the growing flagellum...
November 16, 2017: Biology of the Cell
https://www.readbyqxmd.com/read/29140789/mechanism-and-dynamics-of-inpp5e-transport-into-and-inside-the-ciliary-compartment
#3
Stefanie Kristine Kösling, Eyad Kalawy Fansa, Stefano Maffini, Alfred Wittinghofer
The inositol polyphosphate 5´-phosphatase E (INPP5E) localizes to cilia. We showed that the carrier protein phosphodiesterase 6 delta subunit (PDE6δ) mediates the sorting of farnesylated INPP5E into cilia due to high affinity binding and release by Arl3 GTP. However, the dynamics of INPP5E transport into and inside the ciliary compartment are not fully understood. Here we first investigate the movement of INPP5E using live cell fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) analysis...
August 28, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/29139323/jaboticaba-plinia-peruviana-extract-nanoemulsions-development-stability-and-in-vitro-antioxidant-activity
#4
Letícia Mazzarino, Heloísa da Silva Pitz, Ana Paula Lorenzen Voytena, Adriana Carla Dias Trevisan, Rosa Maria Ribeiro-Do-Valle, Marcelo Maraschin
OBJECTIVE: The aim of this work is to develop and characterize nanoemulsions containing jaboticaba extract (Plinia peruviana) aiming pharmaceutical and cosmetic applications. METHODS: Nanoemulsions were prepared by high-pressure homogenization method using different concentrations of components (oil, surfactant, and extract) and homogenization pressures, in order to optimize the preparation conditions. Both unloaded and extract-loaded nanoemulsions were characterized according their size, polydispersity, zeta potential, pH, morphology, and physical stability...
November 15, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/29137494/trh-receptor-mobility-in-the-plasma-membrane-is-strongly-affected-by-agonist-binding-and-by-interaction-with-some-cognate-signaling-proteins
#5
Radka Moravcova, Barbora Melkes, Jiri Novotny
OBJECTIVES: Extensive research has been dedicated to elucidating the mechanisms of signal transduction through different G protein-coupled receptors (GPCRs). However, relatively little is known about the regulation of receptor movement within the cell membrane upon ligand binding. In this study we focused our attention on the thyrotropin-releasing hormone (TRH) receptor that typically couples to Gq/11 proteins. METHODS: We monitored receptor diffusion in the plasma membrane of HEK293 cells stably expressing yellow fluorescent protein (YFP)-tagged TRH receptor (TRHR-YFP) by fluorescence recovery after photobleaching (FRAP)...
November 14, 2017: Journal of Receptor and Signal Transduction Research
https://www.readbyqxmd.com/read/29136948/gastroprotective-effect-and-mode-of-action-of-methanol-extract-of-sphenodesme-involucrata-var-paniculata-c-b-clarke-munir-lamiaceae-leaves-on-experimental-gastric-ulcer-models
#6
Puthanpura Sasidharan Sreeja, Karuppusamy Arunachalam, Sathyanarayanan Saikumar, Muniyandi Kasipandi, Sivaraj Dhivya, Rajan Murugan, Thangaraj Parimelazhagan
Sphenodesme involucrata var. paniculata (C. B. Clarke) Munir, endemic to South Asia, is used by tribal for alleviation from abdominal disorders, inflammation and body pain. However, the gastroprotective properties of this species have not yet been studied. The leaves of S. involucrata were extracted by Soxhlet extraction using different solvents successively and the extracts were analyzed for antioxidant and anti-Helicobacter pylori activities using different in vitro assays. The chemical composition of methanol extract of S...
November 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29132911/antioxidant-anti-inflammatory-and-gastroprotective-activity-of-filipendula-ulmaria-l-maxim-and-filipendula-vulgaris-moench
#7
Stevan Samardžić, Jelena Arsenijević, Dragana Božić, Marina Milenković, Vele Tešević, Zoran Maksimović
ETHNOPHARMACOLOGICAL RELEVANCE: Meadowsweet (Filipendula ulmaria (L.) Maxim.) and dropwort (Filipendula vulgaris Moench) are herbaceous perennials employed in folk medicine for their antirheumatic, antipyretic and anti-ulcer properties. AIM OF THE STUDY: To assess ethnomedicinal claims through investigation of antioxidant, anti-inflammatory and gastroprotective effects of F. ulmaria and F. vulgaris lyophilized flower infusions (LFIs) as well as the F. vulgaris isolated flavonoids spiraeoside, kaempferol 4'-O-glucoside, astragalin 2''-O-gallate, mixture of hyperoside 2''-O-gallate and isoquercitrin 2''-O-gallate, and a tannin tellimagrandin II...
November 10, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29129982/antioxidant-capacity-and-major-phenol-compounds-of-horticultural-plant-materials-not-usually-used
#8
Stina C M Burri, Anders Ekholm, Åsa Håkansson, Eva Tornberg, Kimmo Rumpunen
Horticultural plant materials not usually used from onion, carrot, beetroot, sea buckthorn, black and red currants as well as a wastewater powder from olive oil production were analyzed for total phenols content (FC), ferric reducing ability of plasma (FRAP), radical scavenging capacity (ABTS), and for major phenolic compounds by HPLC-MS. Antioxidant capacity and phenol content varied significantly between species and cultivars, with extracts of sea buckthorn leaves being superior. In different species, different phenolic compounds were closely associated with FRAP, ABTS and FC...
November 2017: Journal of Functional Foods
https://www.readbyqxmd.com/read/29128698/seminal-plasma-antioxidants-are-directly-involved-in-boar-sperm-cryotolerance
#9
Junwei Li, Isabel Barranco, Asta Tvarijonaviciute, Manuel F Molina, Emilio A Martinez, Heriberto Rodriguez-Martinez, Inmaculada Parrilla, Jordi Roca
Boar ejaculates are ejected in fractions with a specific composition in terms of sperm numbers and seminal plasma (SP), which is reflected in the varying sperm cryotolerance observed among different fractions. As boar sperm are particularly sensitive to oxidative stress, this study evaluated the role of SP antioxidants in the observed differences in sperm cryotolerance among ejaculate fractions. Ten ejaculates from five boars were manually collected in fractions: the first 10 mL of the sperm-rich fraction (SRF), the rest of the SRF and the post-SRF...
October 31, 2017: Theriogenology
https://www.readbyqxmd.com/read/29128095/olive-oil-polyphenols-extracts-inhibit-inflammatory-markers-in-j774a-1-murine-macrophages-and-scavenge-free-radicals
#10
Marwa Abdallah, Stefania Marzocco, Simona Adesso, Mokhtar Zarrouk, Mokhtar Guerfel
Here we evaluate the olive oil antiradical and anti-inflammatory potential through its polyphenols extracts and examine the influence of olive maturity on olive oil quality properties, polyphenols composition and biological potentials. Samples have been obtained from minor Tunisian olive cultivars (Chemchali, Fouji and Zarrazi) at different maturity indices. Principal quality properties were evaluated and polyphenols analysis was carried out by Folin Ciocalteu reagent and HPLC-UV-MS. Antiradical activity was examined by DPPH and FRAP scavenging assays while J774A...
November 8, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/29126990/a-proanthocyanidin-rich-extract-from-cassia-abbreviata-exhibits-antioxidant-and-hepatoprotective-activities-in-vivo
#11
Mansour Sobeh, Mona F Mahmoud, Mohamed A O Abdelfattah, Haroan Cheng, Assem M El-Shazly, Michael Wink
ETHNOPHARMACOLOGICAL RELEVANCE: Cassia abbreviata is a small to medium sized branched umbrella-shaped deciduous tree. It is widely spread in the tropics, especially in Africa, having a long history in traditional medicine for the treatment of numerous conditions such as headaches, diarrhea, constipation, some skin diseases, malaria, syphilis, pneumonia, stomach troubles, uterine pains, and against gonorrhea. AIM OF THE STUDY: We investigated the phytochemical constituents of a root extract from Cassia abbreviata using HPLC-PDA-ESI-MS/MS...
November 7, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29125176/hplc-dad-esi-ms-ms-analysis-of-fruits-from-firmiana-simplex-l-and-evaluation-of-their-antioxidant-and-antigenotoxic-properties
#12
Mosad Ahmed Ghareeb, Tamer Mohamed, Amal Mohamed Saad, Laila Abdel-Ghany Refahy, Mansour Sobeh, Michael Wink
OBJECTIVES: The secondary metabolites of the fruits of Firmiana simplex (L.) were analysed by LC-DAD-ESI-MS/MS; furthermore, we evaluated their antioxidant and antigenotoxic properties. METHODS: The antioxidant activity was investigated using the 2,2'-diphenyl-1-picrylhydrazyl radical (DPPH), the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) and the ferric reducing antioxidant power (FRAP) assays. The antigenotoxic potential was determined via the comet assay...
November 10, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/29119242/dietary-antioxidant-capacity-and-risk-of-type-2-diabetes-in-the-large-prospective-e3n-epic-cohort
#13
Francesca Romana Mancini, Aurélie Affret, Courtney Dow, Beverley Balkau, Fabrice Bonnet, Marie-Christine Boutron-Ruault, Guy Fagherazzi
AIMS/HYPOTHESIS: Recent evidence suggests that oxidative stress may contribute to the pathogenesis of type 2 diabetes. The diet, and especially fruit and vegetables, contains a variety of compounds with antioxidant activity, which may have cumulative/synergistic antioxidant effects. The total antioxidant capacity, an index derived from dietary intake, is a single estimate of antioxidant capacity from all dietary antioxidants. The main aim of this study was to investigate the relationship between total antioxidant capacity and risk of type 2 diabetes...
November 9, 2017: Diabetologia
https://www.readbyqxmd.com/read/29118465/antioxidant-and-anti-inflammatory-activities-in-extracts-from-minke-whale-balaenoptera-acutorostrata-blubber
#14
Mari Johannessen Walquist, Svein Kristian Stormo, Ida-Johanne Jensen, Bjarne Østerud, Karl-Erik Eilertsen
Intake of long-chain omega-3 polyunsaturated fatty acids (LC-n3-PUFA) is commonly recognized to reduce cardiovascular disease (CVD). In previous studies, cold-pressed whale oil (CWO) and cod liver oil (CLO) were given as a dietary supplement to healthy volunteers. Even though CWO contains less than half the amount of LC-n3-PUFA of CLO, CWO supplement resulted in beneficial effects on anti-inflammatory and CVD risk markers compared to CLO. In the present study, we prepared virtually lipid-free extracts from CWO and CLO and evaluated the antioxidative capacity (AOC) and anti-inflammatory effects...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/29116293/antioxidant-effect-of-morus-nigra-on-chagas-disease-progression
#15
Michelly Cristina Montenote, Vithor Zuccaro Wajsman, Yoichi Takaki Konno, Paulo César Ferreira, Regildo Márcio Gonçalves Silva, Altino Luiz Silva Therezo, Luciana Pereira Silva, Luciamáre Perinetti Alves Martins
Considering the widespread popular use of Morus nigra and the amount of scientific information on its antioxidant and anti-inflammatory activity, the effectiveness of this phytotherapeutic compound in the parasitemia progression during the acute phase of Chagas disease and its role in the development of the inflammatory process as well as its effects on the oxidative damage in the chronic phase of infection were evaluated. Thus, 96 male Swiss mice were randomly divided into eight groups, four groups were uninfected controls, and four groups were intraperitoneally infected with 5...
November 6, 2017: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/29109370/olive-olea-europaea-l-biophenols-a-nutriceutical-against-oxidative-stress-in-sh-sy5y-cells
#16
Syed Haris Omar, Philip G Kerr, Christopher J Scott, Adam S Hamlin, Hassan K Obied
Plant biophenols have been shown to be effective in the modulation of Alzheimer's disease (AD) pathology resulting from free radical-induced oxidative stress and imbalance of the redox chemistry of transition metal ions (e.g., iron and copper). On the basis of earlier reported pharmacological activities, olive biophenols would also be expected to have anti-Alzheimer's activity. In the present study, the antioxidant activity of individual olive biophenols (viz. caffeic acid, hydroxytyrosol, oleuropein, verbascoside, quercetin, rutin and luteolin) were evaluated using superoxide radical scavenging activity (SOR), hydrogen peroxide (H₂O₂) scavenging activity, and ferric reducing ability of plasma (FRAP) assays...
October 29, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29098872/bioactive-compounds-content-and-their-biological-properties-of-acetone-extract-of-cuscuta-reflexa-roxb-grown-on-various-host-plants
#17
K Tanruean, P Poolprasert, J Kumla, N Suwannarach, S Lumyong
The present study is aimed to evaluate the total phenolic and flavonoid contents, and free phenolic compounds of acetone extract of Cuscuta reflexa grown on five different hosts: Coccinia grandis, Ficus racemosa, Samanea saman, Streblus asper and Zollingeria dongnaiensis, and to explore the antioxidant activities, α-glucosidase and tyrosinase inhibitory properties of the extracts. The highest level of total phenolic and flavonoid contents were observed in the extract of C. reflexa that was grown on S. asper (65...
November 3, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29091464/effect-of-four-weeks-ingestion-of-tomato-based-carotenoids-on-exercise-induced-inflammation-muscle-damage-and-oxidative-stress-in-endurance-runners
#18
David C Nieman, Courtney L Capps, Christopher R Capps, Zack L Shue, Jennifer E McBride
This double blind, randomized, placebo controlled crossover trial determined if ingestion of a supplement containing a tomato extract with 11 mg lycopene, phytoene, and phytofluene (T-LPP) for 4 weeks would attenuate inflammation, muscle damage, and oxidative stress post-exercise and during recovery from a 2-h running bout that included 30 minutes of -10% downhill running. Study participants ingested the T-LPP supplement or placebo with the evening meal for 4 weeks prior to running 2 h at high intensity. Blood samples and delayed-onset of muscle soreness (DOMS) ratings were taken pre- and post-4-weeks supplementation, and immediately following the 2-h run, and then 1-h-, 24-h-, and 48-h post-run...
November 1, 2017: International Journal of Sport Nutrition and Exercise Metabolism
https://www.readbyqxmd.com/read/29088986/a-comparison-of-native-and-non-urate-total-antioxidant-capacity-of-fasting-plasma-and-saliva-among-middle-aged-and-older-subjects
#19
Anna Gawron-Skarbek, Anna Prymont-Przymińska, Agnieszka Sobczak, Agnieszka Guligowska, Tomasz Kostka, Dariusz Nowak, Franciszek Szatko
OBJECTIVES: As plasma and salivary total antioxidant capacity (TAC) is mainly contributed by uric acid (UA), the present study measures non-urate TAC (Nu-TAC). The aim of the study was to correlate plasma native TAC, Nu-TAC and UA with their salivary analogues, and compare the UA contribution in both body fluids using two different methods. METHODS: The study involved 55 middle-aged and older subjects (66.7 ± 4.5 years). TAC was determined simultaneously with two methods (ferric reducing ability of plasma - FRAP, 2...
October 31, 2017: Redox Report: Communications in Free Radical Research
https://www.readbyqxmd.com/read/29080914/antioxidant-activity-of-omega-3-derivatives-and-their-delivery-via-nanocages-and-nanocones-dft-and-experimental-in-vivo-investigation
#20
Houshang Najafi, Saeed Changizi-Ashtiyani, Meysam Najafi
The antioxidant properties of omega-3 were investigated via experimental in vivo and theoretical methods. For experimental evaluation, oxidative stress was induced by 30 min bilateral renal ischemia and 24 h of reperfusion in male Sprague Dawley rats. The oxidative stress was evaluated through measuring malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP) levels in renal tissue. In theoretical methods, the reaction enthalpies of antioxidant mechanisms of omega-3 were calculated and the effects of NHMe, OMe, OH, Cl, and Me substituents on its antioxidant activity were investigated...
October 28, 2017: Journal of Molecular Modeling
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