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https://www.readbyqxmd.com/read/29792358/in-vitro-activities-of-ceiba-speciosa-a-st-hil-ravenna-aqueous-stem-bark-extract
#1
Juliana Andréa Dörr, Shanna Bitencourt, Luísa Bortoluzzi, Celso Alves, Joana Silva, Stefani Stoll, Susete Pinteus, Aline Augusti Boligon, Roberto Christ Vianna Santos, Stefan Laufer, Rui Pedrosa, Márcia Inês Goettert
Several species of the genus Ceiba (Malvaceae) are ethnopharmacologically used. Thus, this study aimed to investigate the in vitro beneficial properties of the aqueous stem bark extract of Ceiba speciosa. The extract presented a great amount of phenolic compounds (117.4 ± 6.2 mg GAE/g). The antioxidant activity was assessed by DPPH (IC50  = 42.87 μg/mL), ORAC (2351.17 μmol TE/g) and FRAP (235.94 μM FeSO4 /g) methods. In addition, the extract reduced MCF-7 cell viability as assessed by MTT. However, it prevented mitochondrial membrane depolarization and reduced caspase-9 activity induced by hydrogen peroxide...
May 24, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29790302/bioactive-constituents-of-juniperus-turbinata-gussone-from-la-maddalena-archipelago
#2
A Venditti, F Maggi, L Quassinti, M Bramucci, G Lupidi, L Ornano, M Ballero, C Sanna, M Bruno, S Rosselli, A Bianco
In this paper is reported a comprehensive phytochemical study of Juniperus turbinata (Cupressaceae) collected from La Maddalena Archipelago (Sardinia, Italy). Both the essential oil and the ethanolic extract were analyzed. The essential oil appears to belong to a new chemotype compared to other Mediterranean juniper accessions, as it was favored by geographic isolation of the isles. It showed a low content of monoterpene hydrocarbons and α-terpineol, ent-manoyl oxide 1,10-di-epi-cubenol as the major constituents...
May 22, 2018: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29788718/morphology-and-physical-properties-of-hydrophilic-polymer-modified-lipids-in-supported-lipid-bilayers
#3
Yasuhiro Kakimoto, Yoshihiro Tachihara, Yoshiaki Okamoto, Keisuke Miyazawa, Takeshi Fukuma, Ryugo Tero
Lipid molecules such as glycolipids which are modified with hydrophilic biopolymers participate in the biochemical reactions occurring on cell membranes. Their functions and efficiency are determined by the formation of microdomains and their physical properties. We investigated the morphology and properties of domains induced by the hydrophilic-polymer-modified lipid applying poly-ethylene glycol (PEG)-modified lipid as a model modified lipid. We formed supported lipid bilayers (SLBs) using a 0-10 mol % range of PEG-modified lipid concentration (CPEG)...
May 23, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29787901/profiling-of-phenolic-compounds-and-antioxidant-properties-of-european-varieties-and-cultivars-of-vicia-faba-l-pods
#4
Inês M Valente, Margarida R G Maia, Nertila Malushi, Hugo M Oliveira, Lumturi Papa, José A Rodrigues, António J M Fonseca, Ana R J Cabrita
Vicia faba L. pods are a by-product generated from the industrial processing of beans for human and animal consumption. As phenolic compounds may play important roles in health, the present work envisaged the phenolic characterization of seven European varieties and cultivars of V. faba (major and minor) pods and the assessment of their antioxidant activity. The V. faba methanolic extracts were characterized by HPLC-DAD-MS/MS for identification of polyphenolic compounds. The total phenolic content and antioxidant capacity of the extracts were evaluated by colorimetric methods (Folin-Ciocalteu, DPPH scavenging capacity assay, and FRAP assay)...
May 19, 2018: Phytochemistry
https://www.readbyqxmd.com/read/29787846/chemical-study-antioxidant-anti-hypertensive-and-cytotoxic-cytoprotective-activities-of-centaurea-cyanus-l-petals-aqueous-extract
#5
Graziela Bragueto Escher, Jânio Sousa Santos, Neiva Deliberali Rosso, Mariza Boscacci Marques, Luciana Azevedo, Mariana Araújo Vieira do Carmo, Heitor Daguer, Luciano Molognoni, Leonardo do Prado-Silva, Anderson Souza Sant'Ana, Marcia Cristina da Silva, Daniel Granato
This study aimed to optimise the experimental conditions of extraction of the phytochemical compounds and functional properties of Centaurea cyanus petals. The following parameters were determined: the chemical composition (LC-ESI-MS/MS), the effects of pH on the stability and antioxidant activity of anthocyanins, the inhibition of lipid peroxidation, antioxidant activity, anti-hemolytic activity, antimicrobial, anti-hypertensive, and cytotoxic/cytoprotective effect, and the measurements of intracellular reactive oxygen species...
May 19, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29784332/whey-acerola-flavoured-drink-submitted-ohmic-heating-bioactive-compounds-antioxidant-capacity-thermal-behavior-water-mobility-fatty-acid-profile-and-volatile-compounds
#6
Leandro P Cappato, Marcus Vinicius S Ferreira, Jeremias Moraes, Roberto P S Pires, Ramon S Rocha, Ramon Silva, Roberto P C Neto, Maria Inês B Tavares, Mônica Q Freitas, Flavio N Rodrigues, Veronica M A Calado, Renata S L Raices, Marcia C Silva, Adriano G Cruz
Whey acerola-flavoured drink was subjected to Ohmic Heating (OH) under different operational conditions (45, 60, 80 V at 60 Hz and 10, 100, 1000 Hz with 25 V, 65 °C/30 min) and conventional pasteurization (65 °C/30 min). Bioactive compounds (total phenolics, DPPH, FRAP, ACE levels), fatty acid profile, volatile compounds (CG-MS), thermal behaviors (DSC) and water mobility (TD-NMR) were performed. Reduction of frequency (1000-10 Hz) resulted in a lower bioactive compounds and antioxidant capacity of the samples, except for the DPPH values...
October 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29781181/effects-of-phosphatidylserine-containing-supported-lipid-bilayers-on-the-polarization-of-macrophages
#7
Hongxuan Quan, Yongjoon Kim, Hee Chul Park, Hyeong-Cheol Yang
Placement of dental implants initiates inflammatory foreign body response, in which macrophages play a central role and affect the subsequent tissue healing process such as bone formation. The purpose of this study was to fabricate phosphatidylserine (PS)-containing supported lipid bilayers (SLBs) on a titanium surface to regulate the polarization of macrophages, a critical factor that affects following tissue healing and regeneration. The fluorescent recovery after photobleaching (FRAP) images showed that the percentage of PS had a critical influence on the fluidity, and 20% PS had the highest fluidity...
May 20, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29781105/nonmuscle-myosin-ii-in-cardiac-and-skeletal-muscle-cells
#8
Jushuo Wang, Yingli Fan, Jean M Sanger, Joseph W Sanger
De novo assembly of contractile myofibrils begins with the formation of premyofibrils where filaments of non-muscle myosin (NM II), and actin organize in sarcomeric patterns with Z-Bodies containing muscle-specific alpha-actinin. Interactions of muscle specific myosin (MM II) with NM II occur in a nascent myofibril stage that precedes the assembly of mature myofibrils. By the final stage of myofibrillogenesis, the only myosin II present in the mature myofibrils is MM II. In this current study of myofibril assembly, the three vertebrate isoforms of NM II (A, B, and C) and sarcomeric alpha-actinin, ligated to GFP family proteins, were coexpressed in avian embryonic skeletal and cardiac muscle cells...
May 21, 2018: Cytoskeleton
https://www.readbyqxmd.com/read/29777902/chemical-characterization-and-cerebroprotective-effect-of-methanolic-root-extract-of-colebrookea-oppositifolia-in-rats
#9
Gollapalle Lakshminarayanashastry Viswanatha, Marikunte V Venkataranganna, Nunna Bheema Lingeswara Prasad, Shylaja Hanumanthappa
ETHNOPHARMACOLOGICAL RELEVANCE: Colebrookea oppositifolia Smith is one of the extensively used plants to treat neurological conditions such as epilepsy by the various ethnic communities in sub-Himalayan regions of India such as Bhoxa, Tharu and nomadic Gujjars. AIM OF THE STUDY: This study was conducted to evaluate the cerebroprotective effect of C. oppositifolia methanolic root (MeCO) extract in Wistar rats. MATERIALS AND METHODS: The MeCO was characterized for total phenolic content and later subjected for detailed liquid chromatography-mass spectrometry analysis...
May 16, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29773477/selenium-bioaccumulation-and-associated-nutraceutical-properties-in-calocybe-indica-mushroom-cultivated-on-se-enriched-wheat-straw
#10
Himanshi Rathore, Abhishek Sharma, Shalinee Prasad, Satyawati Sharma
Calocybe indica was cultivated on wheat straw enriched with various concentrations of sodium selenite (Na2 SeO3 ), (2.5-40 μg/ml). The content of selenium (Se) in fruit bodies increased linearly by increasing the amount of Na2 SeO3 , although the biomass yield inhibited above 5 μg/ml. The fruit bodies accumulated inorganic Se into organic forms by integrating the Se into proteins (56%-68%), polysaccharides (22%-29%), and nucleic acids (1.4%-2.7%). Maximum protein content (25.31 g/100 g) was observed in fruit bodies harvested from the substrate enriched with 10 μg/ml Se...
May 15, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29773188/the-toxic-effect-of-gallic-acid-on-biochemical-factors-viability-and-proliferation-of-rat-bone-marrow-mesenchymal-stem-cells-was-compensated-by-boric-acid
#11
Mohammad Hussein Abnosi, Somayeh Yari
OBJECTIVE: Gallic acid (GA) and boron are found in many plants. Our previous studies showed 6 ng/ml boric acid (BA) had positive effect on biochemistry of rat bone marrow mesenchymal stem cells (MSCs) and their osteogenic differentiation. Therefore, we investigate the effect of different doses of GA alone and in the presence of BA on MSCs. MATERIALS AND METHODS: the viability of MSCs was assayed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and trypan blue at 12, 24 and 36 h in presence of different concentration of GA...
July 2018: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/29772811/phenolic-compositions-and-antioxidant-activities-differ-significantly-among-sorghum-grains-with-different-applications
#12
Shuyu Shen, Rui Huang, Charlie Li, Wenyan Wu, Honglin Chen, John Shi, Shiguo Chen, Xingqian Ye
Sorghum grains with different applications had different phenolic profiles, which were corresponded to various antioxidant capacities. In this study, total phenolic, proanthocyanidins and flavonoids contents, as well as contents of individual phenolic compounds from sorghum grains with various applications were determined, and their antioxidant capacities were evaluated. Total phenolic contents (TPC) and total proanthocyanidins contents (TPAC) showed strong correlation with antioxidant activities ( r > 0...
May 17, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29772655/antioxidant-activity-and-hepatoprotective-potential-of-quercetin-7-rhamnoside-in-vitro-and-in-vivo
#13
Zhi-Qiang Huang, Pan Chen, Wei-Wei Su, Yong-Gang Wang, Hao Wu, Wei Peng, Pei-Bo Li
Hypericum japonicum is traditionally used as a folk medicine to treat cholestasis and hepatitis. Quercetin 7-rhamnoside (Q7R) is one of the main flavonoid components of Hypericum japonicum and has been rarely studied. The aim of the present study was to evaluate the antioxidant activity and hepatoprotective potential of Q7R. In the in vitro experiments, DPPH, ABTS and ferric reducing antioxidant power (FRAP) assays were first performed to assess the antioxidant properties of Q7R, and then a H₂O₂-induced oxidative damage cellular model was used to determine the cytoprotective and antioxidant properties of Q7R in human liver L-02 cells...
May 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29772520/-valeriana-wallichii-extract-inhibits-tert-booh-induced-oxidative-damage-and-cytotoxicity
#14
Vishal Jain, Ruma Dutta, Doddamane Thippeswamy Maheshwari, Darmendra Kumar Meena, Kshipra Misra, Mysore Sadashiva Yogendra Kumar
Oxidative s tress is well known to be involved in pathophysiology of several disorders. Valeriana wallichii (VW) root extracts is known for its expedient activities but its antioxidant and cytoprotective efficacy need to be explored further. In present study tertiary-butyl hydroperoxide (tert-BOOH) was used to induce oxidative stress in C6 glioma cells. Antioxidant activity of the VW root extracts were evaluated by chemical assays i.e . DPPH, ABTS and FRAP assay. Further effect of VW on tert-BOOH induced oxidative stress and mitochondrial damage was studied...
June 1, 2018: Frontiers in Bioscience (Elite Edition)
https://www.readbyqxmd.com/read/29770169/dietary-total-antioxidant-capacity-and-dietary-polyphenol-intake-and-prevalence-of-metabolic-syndrome-in-polish-adults-a-nationwide-study
#15
Małgorzata Elżbieta Zujko, Anna Waśkiewicz, Anna Maria Witkowska, Danuta Szcześniewska, Tomasz Zdrojewski, Krystyna Kozakiewicz, Wojciech Drygas
Specific classes and subclasses of polyphenols have been studied for their potential effects on noncommunicable diseases, but studies on association between dietary polyphenol intake (DPI) and dietary total antioxidant capacity (DTAC) and MetS (metabolic syndrome) are scarce. Therefore, the aim of this study was to determine associations between DTAC and DPI and the prevalence of MetS and its components in the Polish adult population. Subjects (5690) were participants of the Polish National Multicentre Health Examination Survey (WOBASZ II study) performed in 2013-2014...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29769067/antioxidant-capacity-of-cinnamon-extract-for-palm-oil-stability
#16
Muhammad Zia Shahid, Hafiza Saima, Adeela Yasmin, Muhammad Tahir Nadeem, Muhammad Imran, Muhammad Afzaal
BACKGROUND: Spices and their bioactive components are more promising attractions for their inclusion in diet-based regimes to improve human health. These are sources of natural antioxidants and play an important role in the chemoprevention of diseases and aging. The aim of the current study was to explore the antioxidant potential of cinnamon; a widely used spice throughout the world. METHODS: The current research was aimed to investigate the antioxidant potential of cinnamon extract...
May 16, 2018: Lipids in Health and Disease
https://www.readbyqxmd.com/read/29757201/antioxidant-structure%C3%A2-activity-relationship-analysis-of-five-dihydrochalcones
#17
Xican Li, Ban Chen, Hong Xie, Yuhua He, Dewei Zhong, Dongfeng Chen
The study determined the comparative antioxidant capacities of five similar dihydrochalcones: phloretin, phloridzin, trilobatin, neohesperidin dihydrochalcone, and naringin dihydrochalcone. In the ferric-reducing antioxidant power (FRAP) assay, the antioxidant activities of pairs of dihydrochalcones had the following relationship: phloretin > phloridzin, phloretin > trilobatin, trilobatin > phloridzin, trilobatin > naringin dihydrochalcone, and neohesperidin dihydrochalcone > naringin dihydrochalcone...
May 12, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29751607/effect-of-microwave-vacuum-drying-on-the-drying-characteristics-color-microstructure-and-antioxidant-activity-of-green-coffee-beans
#18
Wenjiang Dong, Ke Cheng, Rongsuo Hu, Zhong Chu, Jianping Zhao, Yuzhou Long
The aim of this study is to investigate the effect of microwave vacuum drying (MVD) on the drying characteristics and quality attributes of green coffee beans. We specifically focused on the effective moisture diffusion coefficient ( Deff ), surface temperature, glass transition temperature ( Tg ), water state, and microstructure. The kinetics of color changes during drying, total phenolic content (TPC), and antioxidant activity (DPPH, FRAP, and ABTS) were also characterized. Microwave power during MVD affected the porosity of coffee beans, their color, TPC, and antioxidant activity...
May 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29751487/preparation-and-antioxidant-activity-of-ethyl-linked-anthocyanin-flavanol-pigments-from-model-wine-solutions
#19
Lingxi Li, Minna Zhang, Shuting Zhang, Yan Cui, Baoshan Sun
Anthocyanin-flavanol pigments, formed during red wine fermentation and storage by condensation reactions between anthocyanins and flavanols (monomers, oligomers, and polymers), are one of the major groups of polyphenols in aged red wine. However, knowledge of their biological activities is lacking. This is probably due to the structural diversity and complexity of these molecules, which makes the large-scale separation and isolation of the individual compounds very difficult, thus restricting their further study...
May 3, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29739583/the-impact-of-high-pressure-processing-on-the-phenolic-profile-hydrophilic-antioxidant-and-reducing-capacity-of-pur%C3%A3-e-obtained-from-commercial-tomato-varieties
#20
Maja Jeż, Wiesław Wiczkowski, Danuta Zielińska, Ireneusz Białobrzewski, Wioletta Błaszczak
The effect of high hydrostatic pressure (HHP) (450-550-650 MPa/5-10-15 min) on polyphenols profile of purée obtained from commercial tomato varieties (Maliniak, Cerise, Black Prince and Lima) was investigated. Individual polyphenols, total phenolic index (TPI) were quantified using a mass spectrometer (HPLC-MS/MS). Photochemiluminescence (PCLACW), cyclic voltammetry (CV) and ferric reducing antioxidant power (FRAP) assays were used to determine the antioxidant capacity of the hydrophilic extract. The results demonstrated that at certain processing conditions, HHP may enhance or decrease the nutritional quality of tomato purée...
September 30, 2018: Food Chemistry
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