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HbA1c assays

Steven D Chessler, Donald E Lee
A 29-year-old woman was referred for new-onset diabetes mellitus after her glycosylated haemoglobin (HbA1c) was found to be 10.2%. Three years earlier, the patient's HbA1c-measured by the same clinical laboratory-had been 5.5%. The newer HbA1c was discordant with fasting glucose levels and a lack of diabetes-associated symptoms. The laboratory reported that their assay methodology remained unchanged and also that no haemoglobin variants were detected. Further investigation, however, revealed, first, that the patient carried a haemoglobin alpha chain mutation (Hb Wayne) that can sometimes cause assay interference and, second, that although the laboratory's assay methodology had not changed, their assay instrument had...
June 14, 2018: BMJ Case Reports
Jagjit Kaur, Cheng Jiang, Guozhen Liu
Measurement of glycosylated hemoglobin (HbA1c) is a gold standard procedure for assessing long term glycemic control in individuals with diabetes mellitus as it gives the stable and reliable value of blood glucose levels for a period of 90-120 days. HbA1c is formed by the non-enzymatic glycation of terminal valine of hemoglobin. The analysis of HbA1c tends to be complicated because there are more than 300 different assay methods for measuring HbA1c which leads to variations in reported values from same samples...
June 7, 2018: Biosensors & Bioelectronics
Gabriel Waari, Joseph Mutai, Joseph Gikunju
Introduction: Medication non-adherence is a common problem facing health care providers treating adult type 2 diabetes mellitus patients. Poor glycaemic control associated with increased morbidity and mortality are resulting consequences. The objective of this study was to assess medication adherence among Type 2 diabetes mellitus patients. Methods: This is a cross-sectional study conducted at Kenyatta National Hospital from November 2015 to January 2016. 290 Type 2 diabetic patients were enrolled...
2018: Pan African Medical Journal
Mary Ellen Vajravelu, Joyce M Lee
PURPOSE OF REVIEW: Because the incidence of type 2 diabetes and prediabetes in children is rising, routine screening of those at risk is recommended. In 2010, the ADA made the recommendation to include hemoglobin A1c (HbA1c) as a diagnostic test for diabetes, in addition to the oral glucose tolerance test or fasting plasma glucose. Our objective was to assess the pediatric literature with regard to HbA1c test performance and discuss advantages and disadvantages of use of the test for diagnostic purposes...
June 4, 2018: Current Diabetes Reports
Daniela Lucchesi, Simona Georgiana Popa, Veronica Sancho, Laura Giusti, Monia Garofolo, Giuseppe Daniele, Laura Pucci, Roberto Miccoli, Giuseppe Penno, Stefano Del Prato
BACKGROUND: High-density lipoproteins (HDLs) can exert anti-atherogenic effects. On top of removing excess cholesterol through reverse cholesterol transport, HDLs play beneficial actions on endothelial function and integrity. In particular, HDLs are strong determinant of endothelial progenitor cells (EPCs) number and function. To gain further insights into such an effect we characterized in vitro functionality of circulating "early" EPCs obtained from 60 type 2 diabetes individuals with low HDL-cholesterol (HDL-C) and 59 with high HDL-C levels...
June 5, 2018: Cardiovascular Diabetology
Eunjin Sohn, Junghyun Kim, Chan-Sik Kim, Kyuhyung Jo, Jin Sook Kim
Mesangial cell proliferation contributes to the development of glomerulosclerosis in diabetic nephropathy. This study was aimed at determining whether Osteomeles schwerinae (OSSC) extract can ameliorate renal damage in Spontaneously Diabetic Torii (SDT) rats. OSSC extract (100 and 250 mg/kg/day) was administered to the SDT rats through oral gavage for 17 weeks. At the end of the experiment, glucose, HbA1c, and albuminuria were measured. In addition, the levels of mesangial proliferation-related proteins were determined by western blotting and immunohistochemistry...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Xingxing He, Yifei Mo, Xiaojing Ma, Lingwen Ying, Wei Zhu, Yufei Wang, Yuqian Bao, Jian Zhou
BACKGROUND: Recent studies have discussed the relationship between body mass index (BMI) and glycated albumin (GA) level. However, the extent of the influence of BMI on GA remains uncertain. We investigated the associations between BMI and GA, glycated hemoglobin A1c (HbA1c ) and GA/HbA1c , and to analyze the influence of obesity on GA, HbA1c , and GA/HbA1c in both Chinese diabetic and non-diabetic populations. METHODS: A total of 2562 participants, including 1177 men and 1385 women (age 20-80 y), were enrolled...
May 23, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Rahmoun Mohammed Nadjib, Ghembaza Amine, Habi Mohammed Amine
The understanding of the relationship between the standard values ​​of glycated hemoglobin (HbA1c) and related parameters of the subject (age, sex, BMI, and complications etc …) could be a good track for following and the screening of diabetes. In this side, we recruited ten non diabetic subjects as witnesses and ninety diabetic type 2. Diabetic subjects were assayed for their blood glucose and glycated hemoglobin levels and a calculation of their body mass index. Our results showed that the diabete is more common in women than men...
May 21, 2018: Diabetes & Metabolic Syndrome
Adel M A Assiri, Hala F M Kamel, Abeer A ALrefai
The interaction of advanced glycation end products (AGE) and their receptors promote vascular complications of diabetes in hemodialysis (HD) patients. The soluble form of the receptor for the advanced glycation end-products (sRAGE) has been studied as a vascular biomarker in various diseases with controversial results. Our aim was to evaluate the association of the serum levels of the AGEs and their receptor sRAGE with cardiovascular disease (CVD) and the cardiovascular risk factors among HD patients. There were 130 HD patients and 80 age and gender matched control subjects were involved; 31...
May 22, 2018: Medical Sciences: Open Access Journal
Nguyen Linh Toan, Nguyen Van Hoan, Doan Viet Cuong, Nguyen Viet Dung, Phan The Dung, Ngo Thu Hang, Do Thi Huyen Dieu, Dang Thanh Chung, Ho Anh Son, Pham Xuan Phong, George Binh Lenon, Doan Van De, Hoang Van Tong
Background: Adipokines are involved in the pathogenesis of metabolic disorders including obesity and type 2 diabetes mellitus (T2DM). This study investigates the levels of leptin, resistin, visfatin, secreted frizzled-related protein 5 (SFRP5), monocyte chemoattractant protein-1 (MCP-1) and retinol-binding protein 4 (RBP4) and their correlations with clinical parameters of overweight and T2DM. Methods: We recruited overweight 50 patients with T2DM, 88 non-overweight patients with T2DM, 29 overweight and 100 non-overweight individuals devoid of T2DM for this study...
2018: Diabetology & Metabolic Syndrome
Clemens Höbaus, Maximilian Tscharre, Carsten Thilo Herz, Gerfried Pesau, Thomas Wrba, Renate Koppensteiner, Gerit-Holger Schernthaner
BACKGROUND AND AIMS: YKL-40 is an inflammatory marker secreted by macrophages and is expressed in atherosclerotic plaques. YKL-40 increases in coronary artery disease (CAD) with poor coronary collateral vessel development. Higher levels are linked to reduced survival in CAD patients. Studies evaluating YKL-40 in patients with peripheral arterial disease (PAD) are scarce. This study aims to elucidate a possible link between YKL-40 and PAD severity as well as cardiovascular long-term mortality...
May 9, 2018: Atherosclerosis
Duncan Thomas, Teresa Seeman, Alan Potter, Peifeng Hu, Eileen Crimmins, Elizabeth Henny Herningtyas, Cecep Sumantri, Elizabeth Frankenberg
Glycated hemoglobin (HbA1c) measured using high-performance liquid chromatography (HPLC) assays with venous blood and dried blood spots (DBS) are compared for 143 paired samples collected in Aceh, Indonesia. Relative to gold-standard venous-blood values, DBS-based values reported by the HPLC are systematically upward biased for HbA1c<8% and the fraction diabetic (HbA1c ≥ 6.5%) is overstated almost five-fold. Inspection of chromatograms from DBS assays indicates the % glycosylated calculated by the HPLC excludes part of the hemoglobin A which is misidentified as a hemoglobin variant...
January 2018: Biodemography and Social Biology
Rania N Sabry, Maged A El Wakeel, Ghada M El-Kassas, Ahmed F Amer, Wael H El Batal, Salwa Refat El-Zayat, Mohamed Abou-El-Asrar
INTRODUCTION: Type 1 diabetes mellitus (T1DM) is one of the most common chronic diseases in children that may be complicated by micro or macrovascular complications. Measurement of the carotid intima-media thickness (CIMT) allows the early detection of atherosclerotic alterations of blood vessels that may complicate T1DM. SUBJECTS AND METHODS: This study is a case-control study. Participants were classified into two groups. The first group included 40 children with T1DM and the second group included 30 matched healthy controls...
April 15, 2018: Open Access Macedonian Journal of Medical Sciences
Minghuan Suo, Dongmei Wen, Weijia Wang, Decai Zhang, Shengnan Xu, Xia Wang, Ting Hu
Background : Hemoglobin (Hb) A1c , a biochemical marker widely used in monitoring diabetes mellitus, can be quantitatively measured by various examining systems. However, significant errors still exist. In this study, we evaluated the HbA1c level in five patients with compound heterozygotes by five different examining systems and our goal is to identify the existence of erroneous HbA1c  measurement. Methods:  Blood samples collected from normal (no hemoglobin variants) and abnormal (compound heterozygotes) patients were analyzed by capillary electrophoresis technique and sequence analysis...
May 2, 2018: Bioscience Reports
Eric Lontchi-Yimagou, Jérôme Legoff, Jean-Louis Nguewa, Philippe Boudou, Eric V Balti, Jean Jacques Noubiap, Vicky Kamwa, Barbara Atogho-Tiedeu, Marcel Azabji-Kenfack, Eric N Djahmeni, Martine Etoa, Gaelle Lemdjo, Vanessa Balla, Mesmin Y Dehayem, Fabienne Foufelle, Jean-Claude Mbanya, Jean-Francois Gautier, Eugene Sobngwi
BACKGROUND: Viruses have been considered as potential triggers for diabetes development. This study sought to assess insulin secretion and insulin sensitivity in Human Herpes Virus type 8 (HHV8) infected and non-infected sub-Saharan African people with diabetes. METHODS: We consecutively enrolled 173 people with non-autoimmune diabetes: 124 with type 2 diabetes (T2D), and 49 with ketosis-prone diabetes (KPD) admitted in hyperglycaemic crisis. KPD was further sub-classified as new-onset ketotic phase KPD (n=34) or non-ketotic phase KPD (n=15)...
April 29, 2018: Journal of Diabetes
Deborah J Wiebe, Ashley C Baker, Yana Suchy, Tammy K Stump, Cynthia A Berg
OBJECTIVE: To examine whether individual differences and day-to-day fluctuations in diabetes goal planning are associated with Type 1 diabetes (T1D) management during late adolescence, and whether lapses in daily diabetes goal planning are more disruptive to diabetes management among those with poorer executive functioning (EF). METHOD: Late adolescents with T1D (N = 236, Mage = 17.77 years) completed survey measures assessing individual differences in levels of diabetes goal planning and adherence, as well as survey and performance-based measures of EF; glycemic control was assessed through glycated hemoglobin (HbA1c) assays...
April 26, 2018: Health Psychology: Official Journal of the Division of Health Psychology, American Psychological Association
Jianxun Feng, Chang Lu, Qin Dai, Junqin Sheng, Min Xu
BACKGROUND/AIMS: Amniotic fluid stem cells (AFSCs) transplantation is a promising therapeutic strategy for diabetic nephropathy. Sirtuin3 (SIRT3) is a novel mitochondrial protective factor. In the present study, we aimed to investigate whether SIRT3 protects against hyperglycemia-induced AFSCs damage and enhances the therapeutic efficiency of AFSCs in diabetic nephropathy. METHODS: To establish the diabetic nephropathy model, db/ db mice were used. AFSCs were obtained and transplanted into the kidney tissue of db/ db mice...
April 19, 2018: Cellular Physiology and Biochemistry
I L Oikonomidis, T K Tsouloufi, N Soubasis, M Kritsepi-Konstantinou
Haemoglobin A1c (HbA1c), the major fraction of glycated haemoglobin, is widely used for the diagnosis and monitoring of diabetes mellitus in human beings. However, there is a paucity of literature on the most reliable methods available for measurement of canine HbA1c. The aim of this study was to validate a new automated capillary electrophoresis assay for canine HbA1c, to generate a reference interval and to assess the overlap performance of the assay for the diagnosis of diabetes mellitus. Eighty-three blood samples treated with EDTA were included in the study, comprising 63 from healthy dogs and 20 from diabetic dogs...
April 2018: Veterinary Journal
Abrar Yousef Almusharraf, Shimaa Eissa, Mohammed Zourob
The authors describe the identification of an effective binding region of aptamers against glycated (HbA1c) and total haemoglobin (tHb) by using a fluorometric assay. Truncation of the originally selected aptamers from 60 to 46 and 34 nucleotides for HbA1c and tHb, respectively, enhances the affinity for their targets. Moreover, shortening the aptamer sequences leads to a better conformational change after target binding which enabled the integration of the aptamers in a graphene oxide (GO)-based fluorometric assay...
April 19, 2018: Mikrochimica Acta
Dalia H El-Lebedy, Alshaymaa A Ibrahim, Ingy O Ashmawy
AIMS: Vaspin and irisin are novel cytokines proposed as potential new biomarkers of insulin resistance and endothelial dysfunction. This work is to investigate circulating levels of vaspin and irisin in patients with type 2 diabetes mellitus (T2DM) with and without cardiovascular disease (CVD) to study potential association with disease risk. MATERIALS AND METHODS: Circulating levels of vaspin and irisin were assayed in serum from 135 T2DM patients (with and without CVD) and 70 control subjects by ELISA...
April 12, 2018: Diabetes & Metabolic Syndrome
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