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HbA1c assays

Thyparambil Aravindakshan Pramodkumar, Ramamoorthy Jayashri, Kuppan Gokulakrishnan, Kaliyaperumal Velmurugan, Rajendra Pradeepa, Ranjit Mohan Anjana, Viswanathan Mohan
OBJECTIVE: 1,5 anhydroglucitol (1,5 AG) is emerging as a marker of short-term glycemic control. We measured levels of 1,5 AG, fructosamine (FA), and glycated hemoglobin (HbA1c) in Asian Indians with different degrees of glucose intolerance. MATERIALS AND METHODS: We recruited 210 individuals with normal glucose tolerance (NGT; n = 60), impaired glucose tolerance (IGT; n = 50), and Type 2 diabetes mellitus (T2DM; n = 100) from a large tertiary diabetes center in Chennai in Southern India...
September 2016: Indian Journal of Endocrinology and Metabolism
Melvin Khee Shing Leow
BACKGROUND AND PURPOSE: Glycated hemoglobin (HbA1c) reflects the cumulative glucose exposure of erythrocytes over a preceding time frame proportional to erythrocyte survival. HbA1c is thus an areal function of the glucose-time curve, an educationally useful concept to aid teaching and clinical judgment. METHODS: An ordinary differential equation is formulated as a parsimonious model of HbA1c. The integrated form yields HbA1c as an area-under-the-curve (AUC) of a glucose-time profile...
July 16, 2016: Acta Informatica Medica: AIM
Majid Valizadeh, Zahra Piri, Farnaz Mohammadian, Koorosh Kamali, Hamid Reza Amir Moghadami
BACKGROUND: Hypovitaminosis D has been associated with the development of gestational diabetes mellitus (GDM) in many observational studies. OBJECTIVES: We report the first study of the impact of prenatal vitamin D supplementation on postpartum dysglycemia in GDM patients in a randomized clinical trial. PATIENTS AND METHODS: Women with GDM at 12 - 32 weeks of gestation were assigned randomly to either the intervention group (in which serum 25-hydroxy vitamin D [25OHD] levels were measured immediately, n = 48) or the control group (in which the serum was stored and assayed at 6 - 12 weeks post-partum, n = 48)...
April 2016: International Journal of Endocrinology and Metabolism
Morten A Karsdal, Kim Henriksen, Federica Genovese, Diana J Leeming, Mette J Nielsen, Bente J Riis, Claus Christiansen, Inger Byrjalsen, Detlef Schuppan
AIMS/HYPOTHESIS: The treatment of type 2 diabetes with full peroxisome proliferator-activated receptor gamma (PPARγ) agonists improves insulin sensitivity, but is associated with weight gain, heart failure, peripheral oedema and bone loss. Endotrophin, the C-terminal fragment of the α3 chain of procollagen type VI (also called Pro-C6), is involved in both adipose tissue matrix remodelling and metabolic control. We established a serum assay for endotrophin to assess if this novel adipokine could identify type 2 diabetic patients who respond optimally to PPARγ agonists, improving the risk-to-benefit ratio...
September 8, 2016: Diabetologia
Yan Chao, Zemin Wan, Xiaobin Wu, Feng Qiu, Xinzhong Wu, Yunxiu Wang, Peifeng Ke, Jianhua Xu, Junhua Zhuang, Xianzhang Huang
Haemoglobinopathies may interfere with the hemoglobin A1 c (HbA1 c) measurement, leading to incorrect diagnosis and inappropriate treatment. It is essential that HbA1 c assays are capable of identifying haemoglobinopathies. We report two cases of haemoglobin New York (HbNY) discovered through HbA1 c analysis using capillary electrophoresis (Capillarys 2 Flex Piercing [C2FP], Sebia). We used these samples to evaluate the ability of three other HbA1 c assays to identify this variant: ion-exchange high performance liquid chromatography (HPLC; Variant II Turbo [VII-T], Bio-Rad); boronate affinity HPLC (Ultra(2), Trinity Biotech); and immunoassay (cobas c501 Tina-quant Generation 3, Roche Diagnostics)...
September 13, 2016: Annals of Clinical Biochemistry
Milton Fabian Suárez-Ortegón, Stela McLachlan, Sarah H Wild, José Manuel Fernández-Real, Caroline Hayward, Ozren Polašek
The metabolic syndrome (MetS) is known to be associated with elevated serum ferritin levels. The possible association with other Fe markers has been less well studied. We aimed to investigate the cross-sectional association of soluble transferrin receptor (sTfR) and ferritin levels with the MetS components, insulin resistance and glycosylated Hb (HbA1C). The sample consisted of 725 adults, aged 19-93 years (284 men, 151 premenopausal and 290 postmenopausal women), from the Croatian island of Vis. Serum sTfR and ferritin levels were measured by immunoturbidimetry and electrochemiluminescence assays, respectively...
October 2016: British Journal of Nutrition
Yuwadee Boonyasit, Wanida Laiwattanapaisal, Orawon Chailapakul, Jenny Emnéus, Arto R Heiskanen
An impedance-based label-free affinity sensor was developed for the recognition of glycated hemoglobin (HbA1c). Interdigitated gold microelectrode arrays (IDAs) were first modified with a self-assembled monolayer of cysteamine followed by cross-linking with glutaraldehyde and subsequent binding of 3-aminophenylboronic acid (APBA), which selectively binds HbA1c via cis-diol interactions. Impedance sensing was demonstrated to be highly responsive to the clinically relevant HbA1c levels (0.1%-8.36%) with a detection and quantitation limit of 0...
October 4, 2016: Analytical Chemistry
Shuichi Otabe, Hitomi Nakayama, Tsuyoshi Ohki, Eri Soejima, Yuji Tajiri, Kentaro Yamada
BACKGROUND: We aimed to determine whether the discrepancy between haemoglobin A1c values determined by high-performance liquid chromatography and enzymatic haemoglobin A1c measurements in diabetic patients was clinically relevant. METHODS: We randomly recruited 1421 outpatients undergoing diabetic treatment and follow-up who underwent at least three haemoglobin A1c measurements between April 2014 and March 2015 at our clinic. In 6369 samples, haemoglobin A1c was simultaneously measured by HA-8160 and MetaboLead (enzymatic assay), and the values were compared...
August 5, 2016: Annals of Clinical Biochemistry
Ju-Ming Lu, Li-Nong Ji, Yu-Feng Li, Quan-Min Li, Shan-Shan Lin, Xiao-Feng Lv, Li Wang, Yuan Xu, Xiao-Hui Guo, Qi-Yu Guo, Li Ma, Jin Du, Ying-Li Chen, Cui-Ling Zhao, Qiu-Lan Zhang, Qi-Mei She, Xiu-Min Jiao, Mei-Hua Lu, Rong-Qing Pan, Ying Gao
AIMS: This study was to determine whether serum glycated albumin (GA) was a better indicator of glycemic control than hemoglobin A1c (HbA1c) when starting a new treatment regimen for type 2 diabetes. METHODS: Newly diagnosed type 2 diabetes patients, or patients who had poor glycemic control with oral hypoglycemic agents, were enrolled at 10 hospitals in Beijing. Serum GA, HbA1c, fasting blood glucose (FBG), and C-peptide were assayed on Days 0, 14, 28, and 91 after treatment...
November 2016: Journal of Diabetes and its Complications
Andreas S Peters, Marieluise Lercher, Thomas H Fleming, Peter P Nawroth, Moritz S Bischoff, Susanne Dihlmann, Dittmar Böckler, Maani Hakimi
OBJECTIVE: Glyoxalase 1 (GLO1) is ubiquitously expressed in the cytosol of the cell and is the major opponent against the reactive metabolite methylglyoxal, which is involved in the development of atherosclerosis. Nondiabetic individuals with an increased hemoglobin A1c (HbA1c) level are at higher risk for development of cardiovascular diseases. As such, this study investigated whether there was an association between reduced GLO1 activity in atherosclerotic lesions of nondiabetic patients with an increased HbA1c level...
October 2016: Journal of Vascular Surgery
Alexandra Fouts, Laura Pyle, Liping Yu, Dongmei Miao, Aaron Michels, Jeffrey Krischer, Jay Sosenko, Peter Gottlieb, Andrea K Steck
OBJECTIVE: To explore whether electrochemiluminescence (ECL) assays can help improve prediction of time to type 1 diabetes in the TrialNet autoantibody-positive population. RESEARCH DESIGN AND METHODS: TrialNet subjects who were positive for one or more autoantibodies (microinsulin autoantibody, GAD65 autoantibody [GADA], IA-2A, and ZnT8A) with available ECL-insulin autoantibody (IAA) and ECL-GADA data at their initial visit were analyzed; after a median follow-up of 24 months, 177 of these 1,287 subjects developed diabetes...
October 2016: Diabetes Care
K Lian, X Guo, Q Huang, Z Tan, C Xia, R Wang, F Yi, W Li, J Liu, Y Li, S Guo, H Wang, Q Ji, L Tao
Aim: This study was to investigate the change of high-molecula-weight (HMW) adiponectin (APN) isoform, the association between type 2 diabetes mellitus (T2DM) and HMW APN isoform, the variation of Disulfide-bond A oxidoreductase-like protein (DsbA-L), the effect of HMW APN isoform on AMP-dependent protein kinase (AMPK) and endothelial nitric oxide synthase (eNOS) in Chinese T2DM. Method: 169 patients aged at (48.7±9.4) years and 107 healthy control subjects aged at (42.6±7.8) years took part in this study...
October 2016: Experimental and Clinical Endocrinology & Diabetes
(no author information available yet)
OBJECTIVE: The Diabetes Control and Complications Trial (DCCT)/Epidemiology of Diabetes Interventions and Complications (EDIC) study has demonstrated the beneficial effect of intensive therapy on atherosclerosis and clinical cardiovascular outcomes, while identifying hyperglycemia as a dominant risk factor for type 1 diabetes. The current analyses evaluate the extent to which glycemic exposure influences long-term changes in established risk factors for cardiovascular disease (CVD) among patients with type 1 diabetes...
September 2016: Diabetes Care
In-Seung Lee, Ki-Suk Kim, Kang-Hoon Kim, Jiyoung Park, Hyeon-Soo Jeong, Yumi Kim, Yun-Cheol Na, Seok-Geun Lee, Kwang Seok Ahn, Jun Hee Lee, Hyeung-Jin Jang
Type 2 diabetes mellitus (T2DM) is a metabolic syndrome that results from target-tissue resistance to insulin. Obesity is the condition of excess body fat accumulation. T2DM and obesity are both associated with hypertension, hyperlipidemia, and abdominal obesity. In Korean medicine, Yangkyuksanhwa-tang (YKSHT) has been prescribed for patients with T2DM. Oral glucose tolerance tests (OGTT), multiplex assays and hemoglobin A1C (HbA1C) assessments were performed to determine the anti-diabetic effects of YKSHT and two major compositions of YKSHT, Lonicera japonica Thunb...
July 14, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Yuichiro Nishida, Megumi Hara, Tatsuhiko Sakamoto, Koichi Shinchi, Sayo Kawai, Mariko Naito, Nobuyuki Hamajima, Aya Kadota, Sadao Suzuki, Rie Ibusuki, Akie Hirata, Miwa Yamaguchi, Nagato Kuriyama, Isao Oze, Haruo Mikami, Michiaki Kubo, Hideo Tanaka
OBJECTIVE: Inflammation is closely involved in the development of type 2 diabetes, and cigarette smoking acts as potent inducer of inflammation. We therefore investigated interactions between inflammation-related gene polymorphisms and cigarette smoking on glycated hemoglobin (HbA1c) in the Japanese general population. METHOD: We conducted a cross-sectional study using data collected from 2619 Japanese (1274 males and 1345 females) 40-69 years of age who participated in baseline survey of the Japan Multi-Institutional Collaborative Cohort (J-MICC) Study (2005-2008)...
June 2016: Preventive Medicine Reports
S Mariani, D Costantini, C Lubrano, S Basciani, C Caldaroni, G Barbaro, E Poggiogalle, L M Donini, A Lenzi, L Gnessi
BACKGROUND AND AIM: Obesity is increasing worldwide and is related to undesirable cardiovascular outcomes. Epicardial fat (EF), the heart visceral fat depot, increases with obesity and correlates with cardiovascular risk. SIRT1, an enzyme regulating metabolic circuits linked with obesity, has a cardioprotective effect and is a predictor of cardiovascular events. We aimed to assess the relationship of EF thickness (EFT) with circulating SIRT1 in patients with obesity. METHODS AND RESULTS: Sixty-two patients affected by obesity and 23 lean controls were studied...
June 16, 2016: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
Ilkka Penttilä, Karri Penttilä, Päivi Holm, Harri Laitinen, Päivi Ranta, Jukka Törrönen, Rainer Rauramaa
The formation of glycohemoglobin, especially the hemoglobin A1c (HbA1c) fraction, occurs when glucose becomes coupled with the amino acid valine in the β-chain of Hb; this reaction is dependent on the plasma concentration of glucose. Since the early 1970s it has been known that diabetics display higher values OF HbA1C because they have elevated blood glucose concentrations. Thus HbA1c has acquired a very important role in the treatment and diagnosis of diabetes mellitus. After the introduction of the first quantitative measurement OF HbA1C, numerous methods for glycohemoglobin have been introduced with different assay principles: From a simple mini-column technique to the very accurate automated high-pressure chromatography and lastly to many automated immunochemical or enzymatic assays...
June 26, 2016: World Journal of Methodology
Heather F de Vries McClintock, Kathleen B Boyle, Kathryn Rooney, Hillary R Bogner
OBJECTIVE: We examined the comparative effectiveness of an integrated intervention for Type 2 diabetes mellitus (T2DM) and depression by employing patient prioritized planning (PPP) to incorporate patients' financial, social, and emotional needs versus an integrated intervention alone. METHODS: A randomized controlled pilot trial randomly assigned 78 patients prescribed pharmacotherapy for T2DM in primary care to an integrated intervention for T2DM and depression employing PPP to incorporate patients' financial, social, and emotional needs (enhanced intervention) versus an integrated intervention alone (basic intervention)...
July 2016: American Journal of Health Behavior
Elena C Craciun, Daniel C Leucuta, Razvan L Rusu, Bianca A David, Victoria Cret, Eleonora Dronca
BACKGROUND: Paraoxonase-1 is an HDL-associated esterase that acts as an anti-atherogenic agent by protecting LDL from oxidation. This study investigates paraoxonase-1 activities in children and adolescents with type 1 diabetes mellitus and possible associations with other biochemical markers. PATIENTS AND METHODS: The study enrolled 82 children and adolescents with type 1 diabetes mellitus and 41 controls with similar age and gender distribution. Serum paraoxonase-1 arylesterase and salt-stimulated paraoxonase activities were assessed by measuring the rates of phenyl acetate and paraoxon hydrolysis, respectively; paraoxonase-1 lactonase activity and oxidized LDL were assessed by a pH-sensitive colorimetric assay and ELISA, respectively...
2016: Acta Biochimica Polonica
Federica Rigoldi, Ludovica Spero, Andrea Dalle Vedove, Alberto Redaelli, Emilio Parisini, Alfonso Gautieri
Enzymatic assays based on Fructosyl Amino Acid Oxidases (FAOX) represent a potential, rapid and economical strategy to measure glycated hemoglobin (HbA1c), which is in turn a reliable method to monitor the insurgence and the development of diabetes mellitus. However, the engineering of naturally occurring FAOX to specifically recognize fructosyl-valine (the glycated N-terminal residue of HbA1c) has been hindered by the paucity of information on the tridimensional structures and catalytic residues of the different FAOX that exist in nature, and in general on the molecular mechanisms that regulate specificity in this class of enzymes...
July 19, 2016: Molecular BioSystems
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