keyword
MENU ▼
Read by QxMD icon Read
search

HbA1c assays

keyword
https://www.readbyqxmd.com/read/29233785/angiotensin-ii-receptor-blocker-valsartan-ameliorates-cardiac-fibrosis-partly-by-inhibiting-mir-21-expression-in-diabetic-nephropathy-mice
#1
Jinyang Wang, Lijun Duan, Yanbin Gao, Shuhong Zhou, Yongming Liu, Suhong Wei, Siqin An, Jing Liu, Liming Tian, Shaocheng Wang
Cardiac fibrosis with diabetic nephropathy (DN) is one of major diabetic complications. miR-21 and MMP-9 were closely associated with fibrosis diseases. Angiotensin II receptor blockers (ARB) have cardioprotective effects. However, it remains unclear whether miR-21 was involved in the mechanism of cardiac fibrosis with DN by target MMP-9 and ARB ameliorates cardiac fibrosis partly by inhibiting miR-21 expression. In this study, In Situ Hybridization(ISH), RT-PCR, cell transfection, western blotting and laser confocal telescope were used, respectively...
December 9, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29222856/homocysteine-as-a-non-classical-risk-factor-for-atherosclerosis-in-relation-to-pharmacotherapy-of-type-2-diabetes-mellitus
#2
Magdalena Borowska, Marzena Dworacka, Hanna Winiarska, Ewa Krzyżagórska
AIMS: The aim of our study was to evaluate which of the pharmacotherapeutic methods that are frequently used to treat type 2 diabetes is associated with the most beneficial profile in relation to pro-atherogenic homocysteine levels. PATIENTS AND METHODS: We measured the serum homocysteine level in 182 patients with type 2 diabetes treated with metformin (89), treated with insulin in combination with metformin (31), receiving sulfonylureas (31) and treated conventionally with insulin (31)...
December 7, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/29172486/a-stable-isotope-dilution-nanoflow-liquid-chromatography-tandem-mass-spectrometry-assay-for-the-simultaneous-detection-and-quantification-of-glyoxal-induced-dna-cross-linked-adducts-in-leukocytes-from-diabetic-patients
#3
Hauh-Jyun Candy Chen, Ya-Lang Chang, Yi-Chun Teng, Chiung-Fong Hsiao, Tsai-Shiuan Lin
Glyoxal is a bifunctional electrophile capable of cross-linking DNA. Although it is present in foods and from the environment, endogenous formation of glyoxal occurs through metabolism of carbohydratesand oxidation of lipids and nucleic acids. Plasma concentrations of glyoxal are elevatedin in diabetes mellitus patients compared to non-diabetics. The most abundant 2'-deoxyribonucleoside adducts cross-linked by glyoxal are dG-gx-dC, dG-gx-dA, and dG-gx-dG. These DNA cross-links can be mutagenic by damaging the integrity of the DNA structure...
November 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29162770/correlation-of-the-blood-test-results-obtained-between-assays-using-microliter-scale-fingertip-blood-samples-collected-with-a-novel-blood-collection-device-and-conventional-venous-blood-assays-a-secondary-publication-in-english
#4
Hajime Iwasawa, Tomoaki Nishimura, Shota Nemoto, Naoki Aikawa, Kiyoaki Watanabe
In this study, we investigated the correlations between biochemical and hematological test results obtained using microliter-scale fingertip blood samples collected with a newly developed blood collection device and those obtained using conventional venous blood. Eighty volunteer subjects were enrolled in this study. Blood samples were drawn from the fingertip of the ring finger by a single puncture, and 60-µL samples were promptly and accurately aspirated into a blood collection chip. Then the chip was tightly sealed in a chip container and was shaken to mix the contents without dispersion...
November 21, 2017: Keio Journal of Medicine
https://www.readbyqxmd.com/read/29160122/reduced-soluble-toll-like-receptors-2-in-type-2-diabetes
#5
Emanuela Zaharieva, Tsvetelina Velikova, Adelina Tsakova, Zdravko Kamenov
BACKGROUND: Soluble forms of Toll-like receptors (sTLR) 2 and 4 exert negative regulatory control on membrane-bound receptor activation. The study estimates the sTLR2 and sTLR4's serum levels in type 2 diabetes (T2D) and evaluates their relationship with metabolic and inflammatory parameters. SUBJECTS AND METHODS: Sixty three patients with T2D and 25 controls were enrolled. sTLR were assayed through ELISA. Inflammatory markers included Interleukin 6 (IL-6), high sensitivity C-reactive protein (hs-CRP) and tumour necrosis factor α...
November 21, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/29159931/targets-and-teamwork-understanding-differences-in-pediatric-diabetes-centers-treatment-outcomes
#6
Timothy C Skinner, Karin S Lange, Hilary Hoey, Henrik B Mortensen, Henk-Jan Aanstoot, Luis Castaňo, Soren Skovlund, Peter Gf Swift, Fergus J Cameron, Harry R Dorchy, Mark R Palmert, Eero Kaprio, Jean-Jacques Robert, Thomas Danne, Andreas Neu, Shlomit Shalitin, Francesco Chiarelli, Giovanni Chiari, Tatsuhiko Urakami, Pål R Njølstad, Premyslawa K Jarosz-Chobot, Edna F Roche, Cintia G Castro-Correia, Mirjana Kocova, Jan Åman, Eugen Schönle, Timothy G Barrett, Lynda Fisher, Carine E de Beaufort
OBJECTIVE: The reason for center differences in metabolic control of childhood diabetes is still unknown. We sought to determine to what extent the targets, expectations, and goals that diabetes care professionals have for their patients is a determinant of center differences in metabolic outcomes. RESEARCH DESIGN AND METHODS: Children, under the age of 11 with type 1 diabetes and their parents treated at the study centers participated. Clinical, medical, and demographic data were obtained, along with blood sample for centralized assay...
November 20, 2017: Pediatric Diabetes
https://www.readbyqxmd.com/read/29155151/hba1c-monitoring-interval-in-patients-on-treatment-for-stable-type-2-diabetes-a-ten-year-retrospective-open-cohort-study
#7
Sachiko Ohde, Gautam A Deshpande, Hiroshi Yokomichi, Osamu Takahashi, Tsuguya Fukui, Zentaro Yamagata
[Aims] This study aims to suggest an informative interval for HbA1c in DM patients with stable glycemic control, based on test characteristics of the HbA1C assay using the signal-to-noise ratio method. [Methods] This was a retrospective, open cohort study. Data were collected between January 2005 to December 2014 at a tertiary-level community hospital in Japan. All adult patients aged under 75 years, with stable glycemic control on a first pharmaceutical regimen for Type II diabetes, and at least two HbA1c measurements after they achieved glycemic stability, were included in the analysis...
November 16, 2017: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/29143878/sensitive-mass-spectrometric-assay-for-determination-of-15-deoxy-%C3%AE-12-14-prostaglandin-j2-and-its-application-in-human-plasma-samples-of-patients-with-diabetes
#8
Jakob Morgenstern, Thomas Fleming, Ivelina Kadiyska, Sebastian Brings, Jan Benedikt Groener, Peter Nawroth, Markus Hecker, Maik Brune
The determination of individual prostaglandins (PG) in humans is mainly performed in urine samples. The quantification of PGs in human plasma could improve the understanding of particular PG species under various physiological and pathological conditions. 15-Deoxy-Δ(12,14)-prostaglandin J2 (15d-PGJ2) is a dehydrated downstream product of PGD2 and is of high interest due to its recently discovered anti-inflammatory effects. Increasing availability of highly sensitive mass spectrometry allows the quantification of low abundant biomarkers like 15d-PGJ2 in human plasma samples...
November 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29122628/a-novel-curcumin-derivative-for-the-treatment-of-diabetic-neuropathy
#9
Daniel J Daugherty, Alexandra Marquez, Nigel A Calcutt, David Schubert
Neuropathy is a common complication of long-term diabetes. Proposed mechanisms of neuronal damage caused by diabetes that are downstream of hyperglycemia and/or loss of insulin signaling include ischemic hypoxia, inflammation and loss of neurotrophic support. The curcumin derivative J147 is a potent neurogenic and neuroprotective drug candidate initially developed for the treatment of neurodegenerative conditions associated with aging that impacts many pathways implicated in the pathogenesis of diabetic neuropathy...
November 6, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/29108839/association-between-a-mir499a-polymorphism-and-diabetic-neuropathy-in-type-2-diabetes
#10
Cinzia Ciccacci, Andrea Latini, Carla Greco, Cristina Politi, Cinzia D'Amato, Davide Lauro, Giuseppe Novelli, Paola Borgiani, Vincenza Spallone
AIMS: Diabetic polyneuropathy (DPN) and cardiovascular autonomic neuropathy (CAN) affect a large percentage of diabetic people and impact severely on quality of life. As it seems that miRNAs and their variations might play a role in these complications, we investigated whether the rs3746444 SNP in the MIR499A gene could be associated with susceptibility to DPN and/or CAN. METHODS: We analyzed 150 participants with type 2 diabetes. DNA was extracted from peripheral blood samples and genotyping was performed by TaqMan genotyping assay...
October 26, 2017: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/29077935/glycation-reduces-the-stability-of-apoai-and-increases-hdl-dysfunction-in-diet-controlled-type-2-diabetes
#11
Sangeeta R Kashyap, Abdullah Osme, Serguei Ilchenko, Makan Golizeh, Kwangwon Lee, Shuhui Wang, James Bena, Stephen F Previs, Jonathan D Smith, Takhar Kasumov
Context: Hyperglycemia plays a key role in the pathogenesis of cardiovascular complications of diabetes. T2DM is associated with high density lipoprotein (HDL) dysfunction and increased degradation of ApoAI. The mechanism(s) of these changes are largely unknown. Objective: To study the role of hyperglycemia-induced glycation on ApoAI kinetics and stability in patients with diet-controlled T2DM. Design: 2H2O-metabolic labeling approach was used to study ApoAI turnover in patients with diet-controlled T2DM (N= 9 (5F), 59...
October 25, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29075072/evaluation-of-coagulation-profile-in-children-with-type-1-diabetes-mellitus-using-rotational-thromboelastometry
#12
Cigdem Binay, Ayse Bozkurt Turhan, Enver Simsek, Ozcan Bor, Olga Meltem Akay
The prothrombotic state in type 1 diabetes mellitus (T1DM) has been reported as a plausible cause of vascular complications. Rotational thromboelastometry (ROTEM) assay enables the global assessment of coagulation status. This study aimed to assess hypercoagulability in children with T1DM using ROTEM. A total of 43 T1DM children (20 females and 23 males) aged 2-18 years and age- and sex-matched 30 healthy control subjects were enrolled in the study group. ROTEM assays [intrinsic TEM (INTEM) and extrinsic TEM (EXTEM)] were used to measure and analyze coagulation time (CT), clot formation time, maximum clot firmness (MCF)...
December 2017: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/29067244/pre-heparin-lipoprotein-lipase-mass-as-a-potential-mediator-in-the-association-between-adiponectin-and-hdl-cholesterol-in-type-2-diabetes
#13
Raelene E Maser, M James Lenhard, Ryan T Pohlig, P Babu Balagopal
AIM: Lipoprotein lipase (LPL) is a major enzyme in lipid metabolism. Dyslipidemia, characterized by decreased high-density lipoprotein cholesterol (HDL-C), is prevalent in persons with type 2 diabetes mellitus (T2DM). The aim of this study was to determine whether pre-heparin LPL mass mediates the association between adiponectin and HDL-C in individuals with T2DM. METHODS: Pre-heparin LPL mass was measured via an enzyme-linked immunosorbent assay, adiponectin by radioimmunoassay, and HDL-C was determined enzymatically...
March 2017: Journal of Clinical & Translational Endocrinology
https://www.readbyqxmd.com/read/29065866/serum-vaspin-concentration-in-elderly-patients-with-type-2-diabetes-mellitus-and-macrovascular-complications
#14
Wei Yang, Yun Li, Tian Tian, Li Wang, Pearl Lee, Qi Hua
BACKGROUND: Adipose tissue, an endocrine organ of the body, is involved in some obesity-related disease states such as insulin resistance, diabetes mellitus, and atherosclerosis. Vaspin is a novel adipocyte with insulin sensitizing effects. In this study, we planned to estimate serum vaspin concentrations as related to glycemic status and the presence of macrovascular complications among elderly patients with type-2 diabetes mellitus (T2DM). METHODS: A total of 230 elderly patients with T2DM were evaluated...
October 24, 2017: BMC Endocrine Disorders
https://www.readbyqxmd.com/read/29032524/patterns-and-levels-of-platelet-glycosylation-in-patients-with-coronary-heart-disease-and-type-2-diabetes-mellitus
#15
Liping Li, Chenxue Qu, Xuelian Wu, Juhua Dai, Yao Lu, Yan Gong, Ran You, Yaqi Liu
Coronary heart disease (CHD) and diabetes mellitus (DM) have high reactivity of platelets and an increased risk of thrombosis. Platelet glycosylation is closely related to platelet function and survival. However, the alteration of platelet glycosylation in CHD and T2DM still remains unknown. Platelet samples were obtained from 55 healthy controls and 102 patients, including 33 CHD, 30 T2DM and 39 CHD complicated with T2DM (CHD + T2DM). Platelet glycosylation was detected using eight-lectin based assay by flow cytometry...
October 14, 2017: Journal of Thrombosis and Thrombolysis
https://www.readbyqxmd.com/read/29028997/serum-quantitative-proteomic-analysis-reveals-soluble-egfr-to-be-a-marker-of-insulin-resistance-in-male-mice-and-humans
#16
Mayu Kyohara, Jun Shirakawa, Tomoko Okuyama, Ayuko Kimura, Yu Togashi, Kazuki Tajima, Hisashi Hirano, Yasuo Terauchi
To identify circulating factors as candidates involved in type 2 diabetes (T2DM), we conducted two different quantitative proteomic analyses: 1) db/db mouse sera were compared with db/+ mouse sera obtained at 4, 8, 12, and 24 weeks of age, and 2) db/db mouse sera from animals treated with liraglutide were compared with sera from animals without liraglutide treatment. 20 and eight proteins were differentially expressed in db/db mouse sera in the first experiment and in db/db mouse sera after liraglutide treatment in the second experiment, respectively...
October 5, 2017: Endocrinology
https://www.readbyqxmd.com/read/29022248/coxsackievirus-b4-as-a-causative-agent-of-diabetes-mellitus-type-1-is-there-a-role-of-inefficiently-treated-drinking-water-and-sewage-in-virus-spreading
#17
Waled M El-Senousy, Adel Abdel-Moneim, Mahmoud Abdel-Latif, Mohamed H El-Hefnawy, Rehab G Khalil
This study proposed to detect the enterovirus (EV) infection in children with type 1 diabetes mellitus (T1D) and to assess the role of insufficiently treated water and sewage as sources of viral spreading. Three hundred and eighty-two serum specimens of children with T1D, one hundred serum specimens of children who did not suffer from T1D as control, and forty-eight water and sewage samples were screened for EV RNA using nested RT-PCR. The number of genome copies and infectious units of EVs in raw and treated sewage and water samples were investigated using real-time (RT)-PCR and plaque assay, respectively...
October 11, 2017: Food and Environmental Virology
https://www.readbyqxmd.com/read/28994284/gastro-resistant-insulin-receptor-binding-peptide-from-momordica-charantia-improved-the-glucose-tolerance-in-streptozotocin-induced-diabetic-mice-via-insulin-receptor-signaling-pathway
#18
Hsin-Yi Lo, Chia-Cheng Li, Feng-Yuan Chen, Jaw-Chyun Chen, Chien-Yun Hsiang, Tin-Yun Ho
Momordica charantia is a commonly used food and has been used for the management of diabetes. Our previous study has identified an insulin receptor (IR)-binding protein (mcIRBP) from Momordica charantia. Here we identified the gastro-resistant hypoglycemic bioactive peptides from protease-digested mcIRBP. By in vitro digestion and IR kinase activity assay, we found that a 9-amino-acid-residue peptide, mcIRBP-9, was a gastro-resistant peptide that enhanced IR kinase activities. mcIRBP-9 activated IR signaling transduction pathway, which resulted in the phosphorylation of IR, the translocation of glucose transporter 4, and the uptake of glucose in cells...
October 17, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28977159/is-fibroblast-growth-factor-23-a-new-cardiovascular-risk-marker-in-gestational-diabetes
#19
Muhammed Kizilgul, Seyfullah Kan, Selvihan Beysel, Mahmut Apaydin, Ozgur Ozcelik, Mustafa Caliskan, Mustafa Ozbek, Seyda Ozdemir, Erman Cakal
OBJECTIVE: This study was designed to compare the serum levels of fibroblast growth factor 23 (FGF23) among patients with gestational diabetes mellitus (GDM) and healthy pregnant women, and to evaluate the association between hormonal and metabolic parameters. SUBJECTS AND METHODS: A total of 82 pregnant women were consecutively enrolled in the study. Of these, 46 were diagnosed as having GDM; the remaining 36 healthy pregnant women served as controls in a cross-sectional study design...
September 18, 2017: Archives of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28960774/the-bdnf-val66met-polymorphism-is-associated-with-lower-bmi-lower-postprandial-glucose-levels-and-elevated-carbohydrate-intake-in-children-and-adolescents
#20
A Kalenda, K Landgraf, D Löffler, P Kovacs, W Kiess, A Körner
BACKGROUND: The amino acid-changing exonic variant rs6265 (Val66Met polymorphism) in the brain-derived neurotrophic factor (BDNF) has been linked to obesity in several genotype-phenotype association studies. OBJECTIVE: To identify metabolic factors by which this effect might be conveyed, we aimed to investigate its correlation with (i) obesity, (ii) metabolic parameters, (iii) serum levels of BDNF and (iv) measures of energy intake in children and adolescents. METHODS: We genotyped the variant in 2131 subjects (age 6-18 years) and checked for an association with obesity...
September 27, 2017: Pediatric Obesity
keyword
keyword
117106
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"