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HbA1c assays

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https://www.readbyqxmd.com/read/27908354/a-test-in-context-hemoglobin-a1c-and-cardiovascular-disease
#1
REVIEW
M Odette Gore, Darren K McGuire
Measurement of glycated hemoglobin (HbA1c), the most widely accepted indicator of long-term glycemic exposure, is central for the diagnosis and management of diabetes mellitus. Levels of HbA1c track epidemiologically with diabetic complications, and glycemic control, as reflected by HbA1c reduction, results in decreased risk of microvascular complications, including diabetic kidney disease, neuropathy, and retinopathy. The relationship between HbA1c reduction and cardiovascular disease prevention in patients with diabetes is more complex, with data from large randomized trials published over the past decade providing clear evidence that lowering of HbA1c per se is an inadequate marker for a therapeutic regimen's impact on cardiovascular outcomes and patient survival...
December 6, 2016: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/27896445/serum-1-5-anhydroglucitol-level-as-a-screening-tool-for-diabetes-mellitus-in-a-community-based-population-at-high-risk-of-diabetes
#2
Yao Wang, Yuexing Yuan, Yanli Zhang, Chenghao Lei, Yi Zhou, Jiajia He, Zilin Sun
AIMS: Early diagnosis of diabetes yields significant clinical benefits; however, currently available diagnostic tools for community-based population are limited. This study aimed to assess the value of serum 1,5-anhydroglucitol (1,5-AG) for the diagnosis and screening of diabetes mellitus in a community-based population at high risk of diabetes. METHODS: In this diagnostic test, 1170 participants underwent a 75-g oral glucose tolerance test. Venous blood samples were collected for fasting blood glucose (FBG), 2-h postprandial blood glucose (PBG), and glycosylated hemoglobin A1c (HbA1c) measurements...
November 28, 2016: Acta Diabetologica
https://www.readbyqxmd.com/read/27878569/assay-of-hemoglobin-a1c-using-lectin-from-aleuria-aurantia
#3
Masato Kabata, Erina Hase, Kouta Kimura, Yuka Kobayashi, Yasushi Ueno, Kazuaki Yoshimune
Hemoglobin A1c (HbA1c) has an N-terminal fructosyl valine on the β-chain, and this modification is caused by the non-enzymatic glycosylation of hemoglobin (Hb). The relative concentration ratio of HbA1c to total Hb is an important biomarker for the diagnosis of diabetes. HbA1c-binding lectins were screened from 29 sources of lectin, and the lectin from Aleuria aurantia (AAL) was revealed to have higher affinity to HbA1c than to Hb. The concentration of HbA1c was determined by lectin-based enzyme-linked immunosorbent assay (ELISA) using the AAL lectin...
December 2016: AMB Express
https://www.readbyqxmd.com/read/27868236/national-continuous-surveys-on-internal-quality-control-for-hba1c-in-306-clinical-laboratories-of-china-from-2012-to-2016-continual-improvement
#4
Tingting Li, Wei Wang, Haijian Zhao, Falin He, Kun Zhong, Shuai Yuan, Zhiguo Wang
BACKGROUND: This study aimed to evaluate whether the quality performance of clinical laboratories in China has been greatly improved and whether Internal Quality Control (IQC) practice of HbA1c has also been changed since National Center for Clinical Laboratories (NCCL) of China organized laboratories to report IQC data for HbA1c in 2012. METHODS: Internal Quality Control information of 306 External Quality Assessment (EQA) participant laboratories which kept reporting IQC data in February from 2012 to 2016 were collected by Web-based EQA system...
November 21, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27853030/the-prevalence-and-characteristics-of-latent-autoimmune-diabetes-in-adults-subset-among-type-two-diabetes-mellitus-patients-in-northern-nigeria
#5
Salisu Babura Muazu, Innocent Okpe, Felicia Anumah
INTRODUCTION: Latent autoimmune diabetes in adult (LADA) is a form of Type 1 diabetes mellitus (T1DM) that occurs in adult or with advancing age. It commonly occurs in people aged ≥30 years and is characterized by initial response to oral hypoglycemic agents, lean body mass, and presence of glutamic acid decarboxylase autoantibody (GAD-Ab). It exhibits rapid deterioration of the pancreatic β-cells secretory function due to the destructive action of the autoantibodies. The prevalence of LADA among T2DM patients varies among population due to different diagnostic criteria, patients' characteristics, the assay used, and genetic predisposition...
October 2016: Annals of African Medicine
https://www.readbyqxmd.com/read/27819307/hyperglycaemia-induced-reciprocal-changes-in-mir-30c-and-pai-1-expression-in-platelets
#6
Mao Luo, Rong Li, Meiping Ren, Ni Chen, Xin Deng, Xiaoyong Tan, Yongjie Li, Min Zeng, Yan Yang, Qin Wan, Jianbo Wu
Type 2 diabetic mellitus (DM2) is associated with accelerated thrombotic complications and is characterized by high levels of plasminogen activator inhibitor-1 (PAI-1). Recent studies show that human platelets have high levels of miR-30c and synthesize considerable active PAI-1. The underlying mechanism of how PAI-1 expression is upregulated in DM2 is poorly understood. We now report that hyperglycaemia-induced repression of miR-30c increases PAI-1 expression and thrombus formation in DM2. Bioinformatic analysis and identification of miRNA targets were assessed using luciferase assays, quantitative real-time PCR and western blots in vitro and in vivo...
November 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27819006/associations-of-a-fabp-with-anthropometric-and-metabolic-indices-and-inflammatory-cytokines-in-obese-patients-with-newly-diagnosed-type-2-diabetes
#7
Guifen Niu, Jian Li, Huaiguo Wang, Yuan Ren, Jie Bai
The study aimed to evaluate the relationship between anthropometric and metabolic indices, inflammatory cytokines, and adipocyte fatty acid-binding protein (A-FABP) in obese patients with newly diagnosed type 2 diabetes. The study included 48 nonobese subjects with newly diagnosed type 2 diabetes, 42 obese subjects with newly diagnosed type 2 diabetes, 30 simple obese subjects, and 30 matched normal subjects. Serum A-FABP was assessed by enzyme-linked immunosorbent assay. Pearson's correlations and multiple linear regression stepwise analysis were used to analyze correlations of A-FABP with anthropometric and metabolic indices and inflammatory cytokines...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27812303/the-influence-of-sample-freezing-at-80-%C3%A2-c-for-2-12-weeks-on-glycated-haemoglobin-hba1c-concentration-assayed-by-hplc-method-on-bio-rad-d-10-%C3%A2-auto-analyzer
#8
Katarzyna Bergmann, Grazyna Sypniewska
INTRODUCTION: The aim of the study was to evaluate the effect of a single freeze/thaw cycle on HbA1c concentrations measured by commercially available HPLC method. MATERIALS AND METHODS: Study included 128 whole blood samples collected from diabetic patients (N = 60) and healthy volunteers (N = 68). HbA1c concentrations were measured in fresh blood samples. Then samples were frozen at - 80 °C for up to 12 weeks. HbA1c was assayed by ion-exchange HPLC method on Bio-Rad D-10(®) analyzer...
October 15, 2016: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/27782779/effects-of-endurance-training-on-the-skeletal-muscle-nitric-oxide-metabolism-in-insulin-independent-type-2-diabetic-men-a-pilot-study
#9
Christian Brinkmann, Benedikt Schulte-Körne, Marijke Grau, Sinja Obels, Roman Kemmerling, Thorsten Schiffer, Wilhelm Bloch, Klara Brixius
BACKGROUND: Increases in the amount of inducible nitric oxide synthase (iNOS) protein and abnormal production of nitric oxide (NO) in skeletal muscle have been suggested to be associated with peripheral insulin resistance in patients with type 2 diabetes mellitus (T2DM). This pilot study analyzed whether a 3-month endurance training can affect iNOS protein and NO metabolite levels in the vastus lateralis muscle of insulin-independent T2DM men, thereby affecting the patients` glycemic control...
October 26, 2016: Metabolic Syndrome and related Disorders
https://www.readbyqxmd.com/read/27781118/optimal-glycemic-and-hemoglobin-a1c-thresholds-for-diagnosing-diabetes-based-on-prevalence-of-retinopathy-in-an-iranian-population
#10
Naser Samadi Aidenloo, Alireza Mehdizadeh, Neda Valizadeh, Mohammad Abbaszadeh, Siavash Qarequran, Hamidreza Khalkhali
BACKGROUND: The use of glycemic thresholds for diabetes diagnosis is controversial. However, no information is available regarding glycemic and glycated hemoglobin (HbA1c) thresholds for detecting diabetic retinopathy (DR) in the Iranian population. OBJECTIVES: The main purpose of the current investigation was to examine the association of fasting plasma glucose (FPG) and HbA1c levels with diabetic retinopathy (DR), and to determine the relevant cut-off levels in an Iranian population...
August 2016: Iranian Red Crescent Medical Journal
https://www.readbyqxmd.com/read/27777960/the-type-2-deiodinase-thr92ala-polymorphism-is-associated-with-worse-glycemic-control-in-patients-with-type-2-diabetes-mellitus-a-systematic-review-and-meta-analysis
#11
Xiaowen Zhang, Jie Sun, Wenqing Han, Yaqiu Jiang, Shiqiao Peng, Zhongyan Shan, Weiping Teng
Objective. Type 2 deiodinase (Dio2) is an enzyme responsible for the conversion of T4 to T3. The Thr92Ala polymorphism has been shown related to an increased risk for developing type 2 diabetes mellitus (T2DM). The aim of this study is to assess the association between this polymorphism and glycemic control in T2DM patients as marked by the HbA1C levels. Design and Methods. The terms "rs225014," "thr92ala," "T92A," or "dio2 a/g" were used to search for eligible studies in the PubMed, Embase, and Cochrane databases and Google Scholar...
2016: Journal of Diabetes Research
https://www.readbyqxmd.com/read/27768851/analysis-of-hba1c-on-an-automated-multicapillary-zone-electrophoresis-system
#12
Niclas Rollborn, Torbjörn Åkerfeldt, Gunnar Nordin, Xiao Yan Xu, Aleksandra Mandic-Havelka, Lars-Olof Hansson, Anders Larsson
Hemoglobin A1c (HbA1c) is a frequently requested laboratory test and there is thus a need for high throughput instruments for this assay. We evaluated a new automated multicapillary zone electrophoresis instrument (Capillarys 3 Tera, Sebia, Lisses, France) for analysis of HbA1c in venous samples. Routine requested HbA1c samples were analyzed immunologically on a Roche c6000 instrument (n = 142) and then with the Capillarys 3 Tera instrument. The Capillarys 3 Tera instrument performed approximately 70 HbA1c tests/hour...
October 21, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/27761143/altered-concentrations-of-copper-zinc-and-iron-are-associated-with-increased-levels-of-glycated-hemoglobin-in-patients-with-type-2-diabetes-mellitus-and-their-first-degree-relatives
#13
Somayeh Atari-Hajipirloo, Neda Valizadeh, Mohammad-Hassan Khadem-Ansari, Yousef Rasmi, Fatemeh Kheradmand
BACKGROUND: The altered levels of some essential trace elements and antioxidant minerals have been observed in diabetic patients. OBJECTIVES: The aim of the present study was to compare the concentrations of essential trace elements, copper (Cu), zinc (Zn), and iron (Fe) in the serum of patients who have type 2 diabetes mellitus (T2DM) with those of their non-diabetic first-degree relatives (FDR) and control subjects. The association between glycated hemoglobin (HbA1c) and levels of metals was also evaluated...
April 2016: International Journal of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27730082/relationship-of-glycemic-control-markers-1-5-anhydroglucitol-fructosamine-and-glycated-hemoglobin-among-asian-indians-with-different-degrees-of-glucose-intolerance
#14
Thyparambil Aravindakshan Pramodkumar, Ramamoorthy Jayashri, Kuppan Gokulakrishnan, Kaliyaperumal Velmurugan, Rajendra Pradeepa, Ranjit Mohan Anjana, Viswanathan Mohan
OBJECTIVE: 1,5 anhydroglucitol (1,5 AG) is emerging as a marker of short-term glycemic control. We measured levels of 1,5 AG, fructosamine (FA), and glycated hemoglobin (HbA1c) in Asian Indians with different degrees of glucose intolerance. MATERIALS AND METHODS: We recruited 210 individuals with normal glucose tolerance (NGT; n = 60), impaired glucose tolerance (IGT; n = 50), and Type 2 diabetes mellitus (T2DM; n = 100) from a large tertiary diabetes center in Chennai in Southern India...
September 2016: Indian Journal of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27708483/glycated-hemoglobin-hba1c-clinical-applications-of-a-mathematical-concept
#15
Melvin Khee Shing Leow
BACKGROUND AND PURPOSE: Glycated hemoglobin (HbA1c) reflects the cumulative glucose exposure of erythrocytes over a preceding time frame proportional to erythrocyte survival. HbA1c is thus an areal function of the glucose-time curve, an educationally useful concept to aid teaching and clinical judgment. METHODS: An ordinary differential equation is formulated as a parsimonious model of HbA1c. The integrated form yields HbA1c as an area-under-the-curve (AUC) of a glucose-time profile...
July 16, 2016: Acta Informatica Medica: AIM
https://www.readbyqxmd.com/read/27679649/the-impact-of-vitamin-d-supplementation-on-post-partum-glucose-tolerance-and-insulin-resistance-in-gestational-diabetes-a-randomized-controlled-trial
#16
Majid Valizadeh, Zahra Piri, Farnaz Mohammadian, Koorosh Kamali, Hamid Reza Amir Moghadami
BACKGROUND: Hypovitaminosis D has been associated with the development of gestational diabetes mellitus (GDM) in many observational studies. OBJECTIVES: We report the first study of the impact of prenatal vitamin D supplementation on postpartum dysglycemia in GDM patients in a randomized clinical trial. PATIENTS AND METHODS: Women with GDM at 12 - 32 weeks of gestation were assigned randomly to either the intervention group (in which serum 25-hydroxy vitamin D [25OHD] levels were measured immediately, n = 48) or the control group (in which the serum was stored and assayed at 6 - 12 weeks post-partum, n = 48)...
April 2016: International Journal of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27631136/serum-endotrophin-identifies-optimal-responders-to-ppar%C3%AE-agonists-in-type-2-diabetes
#17
Morten A Karsdal, Kim Henriksen, Federica Genovese, Diana J Leeming, Mette J Nielsen, Bente J Riis, Claus Christiansen, Inger Byrjalsen, Detlef Schuppan
AIMS/HYPOTHESIS: The treatment of type 2 diabetes with full peroxisome proliferator-activated receptor gamma (PPARγ) agonists improves insulin sensitivity, but is associated with weight gain, heart failure, peripheral oedema and bone loss. Endotrophin, the C-terminal fragment of the α3 chain of procollagen type VI (also called Pro-C6), is involved in both adipose tissue matrix remodelling and metabolic control. We established a serum assay for endotrophin to assess if this novel adipokine could identify type 2 diabetic patients who respond optimally to PPARγ agonists, improving the risk-to-benefit ratio...
January 2017: Diabetologia
https://www.readbyqxmd.com/read/27629909/annals-express-identification-of-hb-new-york-by-hba1c-measurement-using-the-sebia-capillarys-2-flex-piercing-system
#18
Yan Chao, Zemin Wan, Xiaobin Wu, Feng Qiu, Xinzhong Wu, Yunxiu Wang, Peifeng Ke, Jianhua Xu, Junhua Zhuang, Xianzhang Huang
Haemoglobinopathies may interfere with the hemoglobin A1 c (HbA1 c) measurement, leading to incorrect diagnosis and inappropriate treatment. It is essential that HbA1 c assays are capable of identifying haemoglobinopathies. We report two cases of haemoglobin New York (HbNY) discovered through HbA1 c analysis using capillary electrophoresis (Capillarys 2 Flex Piercing [C2FP], Sebia). We used these samples to evaluate the ability of three other HbA1 c assays to identify this variant: ion-exchange high performance liquid chromatography (HPLC; Variant II Turbo [VII-T], Bio-Rad); boronate affinity HPLC (Ultra(2), Trinity Biotech); and immunoassay (cobas c501 Tina-quant Generation 3, Roche Diagnostics)...
September 13, 2016: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/27605239/soluble-transferrin-receptor-levels-are-positively-associated-with-insulin-resistance-but-not-with-the-metabolic-syndrome-or-its-individual-components
#19
Milton Fabian Suárez-Ortegón, Stela McLachlan, Sarah H Wild, José Manuel Fernández-Real, Caroline Hayward, Ozren Polašek
The metabolic syndrome (MetS) is known to be associated with elevated serum ferritin levels. The possible association with other Fe markers has been less well studied. We aimed to investigate the cross-sectional association of soluble transferrin receptor (sTfR) and ferritin levels with the MetS components, insulin resistance and glycosylated Hb (HbA1C). The sample consisted of 725 adults, aged 19-93 years (284 men, 151 premenopausal and 290 postmenopausal women), from the Croatian island of Vis. Serum sTfR and ferritin levels were measured by immunoturbidimetry and electrochemiluminescence assays, respectively...
October 2016: British Journal of Nutrition
https://www.readbyqxmd.com/read/27598723/boronate-modified-interdigitated-electrode-array-for-selective-impedance-based-sensing-of-glycated-hemoglobin
#20
Yuwadee Boonyasit, Wanida Laiwattanapaisal, Orawon Chailapakul, Jenny Emnéus, Arto R Heiskanen
An impedance-based label-free affinity sensor was developed for the recognition of glycated hemoglobin (HbA1c). Interdigitated gold microelectrode arrays (IDAs) were first modified with a self-assembled monolayer of cysteamine followed by cross-linking with glutaraldehyde and subsequent binding of 3-aminophenylboronic acid (APBA), which selectively binds HbA1c via cis-diol interactions. Impedance sensing was demonstrated to be highly responsive to the clinically relevant HbA1c levels (0.1%-8.36%) with a detection and quantitation limit of 0...
October 4, 2016: Analytical Chemistry
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