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https://www.readbyqxmd.com/read/28109203/pedobacter-lusitanus-sp-nov-a-new-bacterial-species-isolated-from-sludge-of-a-deactivated-uranium-mine-in-portugal
#1
Cláudia Covas, Tânia Caetano, Andreia Cruz, Tiago Santos, Liliana Dias, Guenter Klein, Amir Abdulmawjood, Luiz Rodríguez-Alcalá, Lígia Pimentel, Ana Gomes, Ana Cristina Freitas, Alba Garcia-Serrano, Javier Fontecha, Sónia Mendo
Strain NL19T is a Gram negative aerobic bacterium that was isolated from sludge of a deactivated uranium mine in Portugal. 16S rRNA gene sequence analysis revealed that strain NL19T is a member of the genus Pedobacter and closely related to the strains Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and P. hartonius DSM 19033T. It has a DNA G+C content of 40.8 mol%, which agreed with the genus description. The main fatty acids include C16:1 9c, C14:1 9c, C4:0, Iso-C17:0, Iso-C17:0 3-OH, C16:0, Anteiso-C15:0 and Iso-C15:0 3-OH...
January 21, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109200/arcobacter-lekithochrous-sp-nov-a-new-species-isolated-from-a-molluscan-hatchery-in-norway
#2
Ana L Diéguez, Sabela Balboa, Thorolf Magnesen, Jesús L Romalde
Four bacterial strains, LFT 1.7T, LT2C 2.5, LT4C 2.8 and TM 4.6, were isolated from Great scallop (Pecten maximus) larvae and tank seawater in a Norwegian hatchery and characterized by a polyphasic approach including phenotypic, chemotaxonomic and genomic traits. All of them were Gram-negative and motile rods, oxidase and catalase positive and required sea salts for growth. Major fatty acids present were Summed Feature 3 (C16:1ω7c/C16:1 ω6c), Summed Feature 8 (C18:1ω7c or C18:1 ω6c), C16:0, C14:0, Summed Feature 2 (C14:0 3OH/C16:1 iso I), C12:0 3OH and C12:0...
January 19, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28109187/antimicrobial-and-antioxidant-activities-of-saccharomyces-cerevisiae-ifst062013-a-potential-probiotic
#3
Md Fakruddin, Md Nur Hossain, Monzur Morshed Ahmed
BACKGROUND: Probiotic yeast has become a field of interest to scientists in recent years. METHODS: Conventional cultural method was employed to isolate and identify yeast and standard methods were used to determine different probiotic attributes, antimicrobial and antioxidant properties. RESULTS: This study reports potential probiotic properties of a strain of S. cerevisiae IFST 062013 isolated from fruit. The isolate is tolerant to a wide range of temperature and pH, high concentration of bile salt and NaCl, gastric juice, intestinal environment, α-amylase, trypsin and lysozyme...
January 21, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28108409/sol-gel-synthesis-and-characterizations-of-hybrid-chitosan-peg-calcium-silicate-nanocomposite-modified-with-zno-nps-and-e102-for-optical-and-antibacterial-applications
#4
Ahmed M Youssef, Amany M El-Nahrawy, Ali B Abou Hammad
Hybrid Chitosan/Poly ethylene glycol/calcium silicate (CS/PEG/calcium silicate) nanocomposite modified with different two types, zinc oxide nanoparticles (ZnO-NPs) and tartrazine dye (E102) were prepared by sol gel method and the characterization of their structure and biological properties were carried out in order to evaluate the possible use in optical and biomedical fields. The hybrid CS/PEG/calcium silicate complex formations have been established by X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopic analysis...
January 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28108375/the-role-of-pattern-recognition-receptors-in-lung-sarcoidosis
#5
Esmaeil Mortaz, Ian M Adcock, Atefhe Abedini, Arda Kiani, Mehdi Kazempour-Dizaji, Masoud Movassaghi, Johan Garssen
Sarcoidosis is a granulomatous disorder of unknown etiology. Infection, genetic factors, autoimmunity and an aberrant innate immune system have been explored as potential causes of sarcoidosis. The etiology of sarcoidosis remains unknown, and it is thought that it might be caused by an infectious agent in a genetically predisposed, susceptible host. Inflammation results from recognition of evolutionarily conserved structures of pathogens (Pathogen-associated molecular patterns, PAMPs) and/or from reaction to tissue damage associated patterns (DAMPs) through recognition by a limited number of germ line-encoded pattern recognition receptors (PRRs)...
January 17, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28107612/hemin-binding-by-porphyromonas-gingivalis-strains-is-dependent-on-the-presence-of-a-lps
#6
Minnie Rangarajan, Joseph Aduse-Opoku, Nikolay A Paramonov, Ahmed Hashim, Michael A Curtis
Porphyromonas gingivalis is a Gram-negative black pigmenting anaerobe unable to synthesise haem (Fe (II)-protoporphyrin IX) or hemin (Fe(III)-protoporphyrin IX-Cl) which are important growth/virulence-factors, and must therefore derive them from the host P. gingivalis expresses several proteinaceous hemin -binding-sites which are important in the binding/ transport of haem/hemin from the host. P. gingivalis also synthesises several virulence factors, namely cysteine-proteases Arg- and Lys-gingipains and two lipopolysaccharides (LPS), O-LPS and A-LPS...
January 20, 2017: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/28107379/experimental-and-in-silico-investigation-of-anti-microbial-activity-of-1-chloro-2-isocyanatoethane-derivatives-of-thiomorpholine-piperazine-and-morpholine
#7
Charles O Nwuche, Oguejiofo T Ujam, Akachukwu Ibezim, Ifeoma B Ujam
The Antibiogram properties of 1-chloro-2-isocyanatoethane derivatives of thiomorpholine (CTC), piperazine (CPC) and morpholine (CMC) were evaluated by the approved agar well diffusion, the minimum inhibitory concentration (MIC) and in silico techniques. A total of fourteen microbial cultures consisting of ten bacteria and four yeast strains were used in the biological study while affinity of the compounds for DNA gyrase, a validated antibacterial drug target, was investigated by docking method. Results indicate that both thiomorpholine and piperazine had zero activity against the Gram negative organisms tested...
2017: PloS One
https://www.readbyqxmd.com/read/28106310/mechanism-of-type-iii-protein-secretion-regulation-of-flha-conformation-by-a-functionally-critical-charged-residue-cluster
#8
Marc Erhardt, Paige Wheatley, Eun A Kim, Takanori Hirano, Yang Zhang, Mayukh K Sarkar, Kelly T Hughes, David F Blair
The bacterial flagellum contains a specialized secretion apparatus in its base that pumps certain protein subunits through the growing structure to their sites of installation beyond the membrane. A related apparatus functions in the injectisomes of gram-negative pathogens to export virulence factors into host cells. This mode of protein export is termed type-III secretion (T3S). Details of the T3S mechanism are unclear. It is energized by the proton gradient; here, a mutational approach was used to identify proton-binding groups that might function in transport...
January 20, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28106112/a-conserved-tlr5-binding-and-activation-hot-spot-on-flagellin
#9
Wan Seok Song, Ye Ji Jeon, Byeol Namgung, Minsun Hong, Sung-Il Yoon
Flagellin is a bacterial protein that polymerizes into the flagellar filament and is essential for bacterial motility. When flagellated bacteria invade the host, flagellin is recognized by Toll-like receptor 5 (TLR5) as a pathogen invasion signal and eventually evokes the innate immune response. Here, we provide a conserved structural mechanism by which flagellins from Gram-negative γ-proteobacteria and Gram-positive Firmicutes bacteria bind and activate TLR5. The comparative structural analysis using our crystal structure of a complex between Bacillus subtilis flagellin (bsflagellin) and TLR5 at 2...
January 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28105330/genetic-markers-associated-with-resistance-to-beta-lactam-and-quinolone-antimicrobials-in-non-typhoidal-salmonella-isolates-from-humans-and-animals-in-central-ethiopia
#10
Tadesse Eguale, Josephine Birungi, Daniel Asrat, Moses N Njahira, Joyce Njuguna, Wondwossen A Gebreyes, John S Gunn, Appolinaire Djikeng, Ephrem Engidawork
BACKGROUND: Beta-lactam and quinolone antimicrobials are commonly used for treatment of infections caused by non-typhoidal Salmonella (NTS) and other pathogens. Resistance to these classes of antimicrobials has increased significantly in the recent years. However, little is known on the genetic basis of resistance to these drugs in Salmonella isolates from Ethiopia. METHODS: Salmonella isolates with reduced susceptibility to beta-lactams (n = 43) were tested for genes encoding for beta-lactamase enzymes, and those resistant to quinolones (n = 29) for mutations in the quinolone resistance determining region (QRDR) as well as plasmid mediated quinolone resistance (PMQR) genes using PCR and sequencing...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28105270/1-2-4-triazolidine-3-thiones-as-narrow-spectrum-antibiotics-against-multidrug-resistant-acinetobacter-baumannii
#11
William M Huggins, Bradley M Minrovic, Brendan W Corey, Anna C Jacobs, Roberta J Melander, Roger D Sommer, Daniel V Zurawski, Christian Melander
With only two new classes of antibiotics developed in the last 40 years, novel antibiotics are desperately needed to combat the growing problem of multidrug-resistant and extensively drug resistant bacteria, particularly Gram-negative bacteria. Described in this letter is the synthesis and antibiotic activity of 1,2,4-triazolidine-3-thiones as narrow spectrum antibiotics. Optimization of the 1,2,4-triazolidine-3-thione scaffold identified a small molecule with potent antibiotic activity against multiple strains of multidrug-resistant and extensively drug-resistant Acinetobacter baumannii...
January 12, 2017: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28104786/identification-of-a-chondroitin-synthase-from-an-unexpected-source-the-green-sulfur-bacterium-chlorobium-phaeobacteroides
#12
Dixy E Green, Paul L DeAngelis
Glycosaminoglycans [GAGs] are known to be present in all animals as well as some pathogenic microbes. Chondroitin sulfate is the most abundant GAG in mammals where it has various structural and adhesion roles. The Gram-negative bacteria Pasteurella multocida Type F and Escherichia coli K4 produce extracellular capsules composed of unsulfated chondroitin or a fructosylated chondroitin, respectively. Such polysaccharides that are structurally related to host molecules do not generally provoke a strong antibody response thus are thought to be employed as molecular camouflage during infection...
January 19, 2017: Glycobiology
https://www.readbyqxmd.com/read/28104665/draft-genome-sequences-of-three-aeromonas-hydrophila-isolates-from-catfish-and-tilapia
#13
Hasan C Tekedar, Salih Kumru, Safak Kalindamar, Attila Karsi, Geoffrey C Waldbieser, Tad Sonstegard, Steven G Schroeder, Mark R Liles, Matt J Griffin, Mark L Lawrence
Aeromonas hydrophila is a Gram-negative bacterium that is particularly adapted to freshwater environments and can cause severe infections in fish and humans. Here, we report the draft genomes of three A. hydrophila catfish and tilapia isolates.
January 19, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28104513/a-multi-center-study-of-bacterial-blood-stream-infections-in-pediatric-allogeneic-hematopoietic-cell-transplantation-recipients-the-role-of-acute-gastrointestinal-graft-versus-host-disease
#14
Prakash Satwani, Jason L Freedman, Sonali Chaudhury, Zhezhen Jin, Anya Levinson, Marc D Foca, Jennifer Krajewski, Indira Sahdev, Mala Kiran Talekar, Aliza Gardenswartz, Justin Silverman, Meghan Hayes, Christopher C Dvorak
Blood stream infections (BSI) caused by enteric organisms are associated with a particularly high mortality rate in allogeneic hematopoietic cell transplantation (AlloHCT) recipients. We conducted a retrospective multicenter study aiming to analyze the risk factors associated with antibiotic resistance and impact of BSI on transplant related mortality (TRM) in children following alloHCT. During the study period from 2004 to 2014, 395 children (mean age 9.4 years) with at least one BSI were included. The incidences of resistant gram-negative rods (GNR) were 20...
January 16, 2017: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/28103779/flavone-analogues-as-antimicrobial-agents
#15
Kamlesh Kumar Naik, Sivakumar Thangavel, Afroze Alam
BACKGROUND: Most of the available antimicrobial drugs have developed resistance, some of them suffer from severe toxicity, side effects. So, there is a need to discover novel compound(s) which should not only be potent, but also less toxic and cost effective. OBJECTIVES: The aim of the study is to develop new synthetic antimicrobial agents (Anti-bacterial and anti-fungal) such as 3-substituted flavone/flavanone derivatives, which should be significantly potent with low toxicity...
January 18, 2017: Recent Patents on Inflammation & Allergy Drug Discovery
https://www.readbyqxmd.com/read/28102305/structure-and-membrane-interactions-of-the-homodimeric-antibiotic-peptide-homotarsinin
#16
Rodrigo M Verly, Jarbas M Resende, Eduardo F C Junior, Mariana T Q de Magalhães, Carlos F C R Guimarães, Victor H O Munhoz, Marcelo Porto Bemquerer, Fábio C L Almeida, Marcelo M Santoro, Dorila Piló-Veloso, Burkhard Bechinger
Antimicrobial peptides (AMPs) from amphibian skin are valuable template structures to find new treatments against bacterial infections. This work describes for the first time the structure and membrane interactions of a homodimeric AMP. Homotarsinin, which was found in Phyllomedusa tarsius anurans, consists of two identical cystine-linked polypeptide chains each of 24 amino acid residues. The high-resolution structures of the monomeric and dimeric peptides were determined in aqueous buffers. The dimer exhibits a tightly packed coiled coil three-dimensional structure, keeping the hydrophobic residues screened from the aqueous environment...
January 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28102062/the-role-of-il-17-in-a-lipopolysaccharide-induced-rhinitis-model
#17
Jun Sang Bae, Ji Hye Kim, Eun Hee Kim, Ji Hun Mo
PURPOSE: Lipopolysaccharide (LPS) is a cell wall component of Gram-negative bacteria and important for pro-inflammatory mediators. This study aimed to establish a rhinitis model using ovalbumin (OVA) and LPS in order to evaluate the role of interleukin (IL)-17 in the pathogenesis of an LPS-induced non-eosionophilic rhinitis model. METHODS: Mice were divided into 4 groups and each group consisted of 10 mice (negative control group, allergic rhinitis model group, 1-μg LPS treatment group, and 10-μg LPS treatment group)...
March 2017: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/28101334/implementation-of-short-incubation-maldi-tof-ms-identification-from-positive-blood-cultures-in-routine-diagnostics-and-effects-on-empiric-antimicrobial-therapy
#18
Robin Köck, Jörg Wüllenweber, Dagmar Horn, Christian Lanckohr, Karsten Becker, Evgeny A Idelevich
BACKGROUND: Results of blood culture (BC) diagnostics should be swiftly available to guide treatment of critically ill patients. Conventional BC diagnostics usually performs species identification of microorganisms from mature solid medium colonies. Species identification might be speed up by using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) of biomass from shortly incubated solid media. METHODS: This single-center analysis compared the applicability of MALDI-TOF-based species identification from shortly incubated cultures in laboratory routine vs...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28100574/clostridium-hastiforme-bacteraemia-secondary-to-pyometra-in-a-64-year-old-woman
#19
Matilde Ørum, David Fuglsang-Damgaard, Hans Linde Nielsen
The Gram-negative, rod-shaped, anaerobe bacteria Tissierella praeacuta was first described in 1908 by Tissier. However, during the past decade, Clostridium hastiforme has been recognised as a later synonym of T. praeacuta. C. hastiforme/T. praeacuta has only rarely been described in previous literature as a cause of human infection. We present here a case report of C. hastiforme/T. praeacuta bacteraemia secondary to pyometra in a 64-year-old woman with a history of multiple sclerosis and an intrauterine device inserted three decades earlier...
January 18, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28099062/rectal-carriage-of-extended-spectrum-beta-lactamase-and-carbapenemase-producing-gram-negative-bacilli-in-intensive-care-units-in-tunisia
#20
Samia Hammami, Chaima Dahdeh, Kelthoum Mamlouk, Sana Ferjeni, Elaa Maamar, Zeineb Hamzaoui, Mabrouka Saidani, Salma Ghedira, Mohamed Houissa, Amin Slim, Ilhem Boutiba-Ben Boubaker
This study was conducted to evaluate the rate of fecal carriage of Gram-negative bacilli (GNB) resistant to third-generation cephalosporins (third GC) in patients hospitalized in the intensive care unit (ICU) of Charles Nicolle Hospital of Tunis and to identify the enzymatic mechanisms involved. From February to April 2014, rectal swabs were collected from all patients (n = 38) at admission and once weekly thereafter to identify acquisition. They were cultured on desoxycholate-lactose-agar plates supplemented with cefotaxime (2 mg/L)...
January 18, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
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