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Mehran Peyvasteh, Shahnam Askarpour, Nasrollah Ostadian, Mohammad-Reza Moghimi, Hazhir Javaherizadeh
Background: Hirschsprung's disease is the most common cause of pediatric intestinal obstruction. Contrast enema is used for evaluation of the patients with its diagnosis. Aim: To evaluate sensitivity, specificity, positive predictive value, and negative predictive value of radiologic findings for diagnosis of Hirschsprung in patients underwent barium enema. Methods: This cross sectional study was carried out in Imam Khomeini Hospital for one year starting from 2012, April...
July 2016: Arquivos Brasileiros de Cirurgia Digestiva: ABCD, Brazilian Archives of Digestive Surgery
Alexander von Luhmann, Heidrun Wabnitz, Tilmann Sander, Klaus-Robert Muller
OBJECTIVE: For the further development of the fields of telemedicine, neurotechnology and Brain-Computer Interfaces (BCI), advances in hybrid multimodal signal acquisition and processing technology are invaluable. Currently, there are no commonly available hybrid devices combining bio-electrical and bio-optical neurophysiological measurements (here Electroencephalography (EEG) and functional Near Infrared Spectroscopy (fNIRS)). Our objective was the design of such an instrument, and that in a miniaturized, customizable and wireless form...
September 9, 2016: IEEE Transactions on Bio-medical Engineering
J E Nobles, V Zagorodnii, A Hutchison, Z Celinski
We report the development of a test setup designed to provide a variable frequency biasing signal to a vector network analyzer (VNA). The test setup is currently used for the testing of liquid crystal (LC) based devices in the microwave region. The use of an AC bias for LC based devices minimizes the negative effects associated with ionic impurities in the media encountered with DC biasing. The test setup utilizes bias tees on the VNA test station to inject the bias signal. The square wave biasing signal is variable from 0...
August 2016: Review of Scientific Instruments
Karthiyaini Damodharan, Sasikumar Arunachalam Palaniyandi, Seung Hwan Yang, Joo-Won Suh
We characterized the probiotic properties of Lactobacillus helveticus strains KII13 and KHI1 isolated from fermented cow milk by in vitro and in vivo studies. The strains exhibited tolerance to simulated oro-gastrointestinal (OGT) condition, adherence to Caco-2 cells, and antimicrobial activity. Both the L. helveticus strains produced bioactive tripeptides isoleucyl-prolyl-proline (IPP) and valyl-prolyl-proline (VPP) during fermentation of milk. KII13 showed higher in vitro cholesterol-lowering activity (47%) compared to KHI1 (28%) and L...
July 19, 2016: Journal of Microbiology and Biotechnology
Shiro Miyayama, Masashi Yamashiro, Keiichi Nagai, Akira Yokka, Miki Yoshida, Naoko Sakuragawa
AIM: To evaluate the performance of novel virtual parenchymal perfusion (VPP) software in conventional transcatheter arterial chemoembolization (cTACE) for hepatocellular carcinoma (HCC). METHODS: VPP was retrospectively applied to 43 HCCs treated with cTACE. The virtual embolized area (VEA) was estimated after positioning a virtual injection point on images of non-selective cone-beam computed tomography during hepatic arteriography (CBCTHA), at the same position in superselective cTACE...
June 28, 2016: Hepatology Research: the Official Journal of the Japan Society of Hepatology
Jun Li, Cangran Guo, Zhongshuai Wang, Kai Gao, Xudong Shi, Jing Liu
BACKGROUND: We proposed a method of using electrical stimulation for treatment of malignant melanoma through directly spray-printing liquid metal on skin as soft electrodes to deliver low intensity, intermediate frequency electric fields. METHODS: With patterned conductive liquid metal components on mice skin and under assistance of a signal generator, a sine wave electrical power with voltage of 5 V and 300 kHz could be administrated on treating malignant melanoma tumor...
December 2016: Clinical and Translational Medicine
Christoffer Karlsson, James Nicholas, Drew Evans, Maria Forsyth, Maria Strømme, Martin Sjödin, Patrick C Howlett, Cristina Pozo-Gonzalo
Liquid-solution polymerization and vapor-phase polymerization (VPP) have been used to manufacture a series of chloride- and tosylate-doped poly(3,4-ethylenedioxythiophene) (PEDOT) carbon paper electrodes. The electrochemistry, specific capacitance, and specific charge were determined for single electrodes in 1-ethyl-3-methylimidazolium dicyanamide (emim dca) ionic liquid electrolyte. VPP-PEDOT exhibits outstanding properties with a specific capacitance higher than 300 F g(-1) , the highest value reported for a PEDOT-based conducting polymer, and doping levels as high as 0...
August 23, 2016: ChemSusChem
Lien-Kai Chang, Mi-Ching Tsai
Most screw-thread-type ultrasonic motors are designed to be two-phase driven. This paper aims to present a novel single phase driven design that generates the required wobble motion, thus significantly simplifying the driving circuit of the ultrasonic motor. The proposed single-phase driven screw-thread-type ultrasonic motor works with two orthogonal bending modes generated by an asymmetric stator design. The novel stator design can improve the vibration displacement and further enhance the performance of the single phase driven motor...
May 2016: Review of Scientific Instruments
Maryam Khoshouei, Stefan Pfeffer, Wolfgang Baumeister, Friedrich Förster, Radostin Danev
Cryo-electron tomography (CET) and subtomogram analysis allow studying the structures of macromolecular complexes in their natural context. The radiation sensitivity of vitrified biological specimens and the resulting low signal-to-noise ratio (SNR) in CET limit the amount of structural information that can be mined from tomographic data. The Volta phase plate (VPP) has emerged as an effective means to increase the SNR and hence contrast compared to 'conventional' defocus-based phase contrast transmission electron microscopy (CTEM)...
May 25, 2016: Journal of Structural Biology
Petr Chlanda, Elena Mekhedov, Hang Waters, Cindi L Schwartz, Elizabeth R Fischer, Rolf J Ryham, Fredric S Cohen, Paul S Blank, Joshua Zimmerberg
Influenza A virus hemagglutinin (HA) changes conformation and drives membrane fusion of viral and endosomal membrane at low pH. Membrane fusion proceeds through an intermediate called hemifusion(1,2). For viral fusion the hemifusion structures are not determined(3). Here, influenza virus-like particles (VLP)(4) carrying wild-type (WT) HA or HA hemifusion mutant G1S(5) and liposome mixtures were studied at low pH by Volta phase plate (VPP) cryo-electron tomography (cET) which improves signal-to-noise ratio close to focus...
2016: Nature Microbiology
Raúl Menor Almagro, Juan Francisco Rodríguez Gutiérrez, María Auxiliadora Martín-Martínez, María José Rodríguez Valls, Concepción Aranda Valera, José Luís de la Iglesia Salgado
OBJECTIVE: To determine the dilution titles at antinuclear antibodies (ANA) by indirect immunofluorescence observed in cell substrate HEp-2 and its association with the diagnosis of systemic connective tissue disease in ANA test requested by a Rheumatology Unit. METHOD: Samples of patients attended for the first time in the rheumatology unit, without prior ANA test, between January 2010 and December 2012 were selected. The dilution titers, immunofluorescence patterns and antigen specificity were recorded...
May 21, 2016: Reumatología Clinica
Alice M Proverbio, Jessica Galli
Event-related potentials (ERPs) were recorded in 26 right-handed students while they detected pictures of animals intermixed with those of familiar objects, faces and faces-in-things (FITs). The face-specific N170 ERP component over the right hemisphere was larger in response to faces and FITs than to objects. The vertex positive potential (VPP) showed a difference in FIT encoding processes between males and females at frontal sites; while for men, the FIT stimuli elicited a VPP of intermediate amplitude (between that for faces and objects), for women, there was no difference in VPP responses to faces or FITs, suggesting a marked anthropomorphization of objects in women...
September 2016: Social Cognitive and Affective Neuroscience
Susan Faja, Geraldine Dawson, Elizabeth Aylward, Ellen M Wijsman, Sara Jane Webb
OBJECTIVE: To test differences in neural sensitivity to facial expressions, including expressions with open versus closed mouths, exhibited by (1) adults with autism spectrum disorder (ASD) compared to neurotypical adults, and by (2) short versus long serotonin transporter allele (SLC6A4) carriers. METHODS: Event related potentials (ERPs) to happy, fearful, and neutral expressions were collected from neurotypical adults (n=25) and adults with ASD (n=27)-of whom 32 had short and 13 had homozygous long SLC6A4 alleles...
June 2016: Clinical Neurophysiology: Official Journal of the International Federation of Clinical Neurophysiology
Renny Edwin Fernandez, Anil Koklu, Amin Mansoorifar, Ali Beskok
We report dielectrophoretic (DEP) assembly of biological cells and microparticles using platinum-black electrodeposited conductive textile fiber. The three-dimensional conductive structures with high aspect ratios were found to facilitate high electric field regions, as revealed by scanning electron microscope characterization. The effective conducting area (Aeff) and its stability of thread electrodes were estimated using electrochemical methods. Potential of platinum black electrodeposited thread as 3-D electrodes for creating high gradient electrical field for dielectrophoretic assembly of microspheres and Saccharomyces cerevisiae (yeast cells) into 1D and two-dimensional structures over long ranges under the application of low voltages (4-10 Vpp) has been demonstrated...
May 2016: Biomicrofluidics
Farzin Forooghian, Peter J Kertes, Kenneth T Eng, David A Albiani, Andrew W Kirker, Andrew B Merkur, Nader Fallah, Sijia Cao, Jing Cui, Chris Or, Joanne A Matsubara
OBJECTIVE: Our previous work has shown that, after intravitreal bevacizumab (IVB) administration, decreases in the levels of vascular endothelial growth factor (VEGF)-A and placental growth factor (PlGF), along with increases in the levels of interleukin (IL)-8 and transforming growth factor (TGF)-β2, can be observed. It is not yet known if similar changes occur after intravitreal ranibizumab (IVR). The purpose of this study was to examine intraocular cytokine changes after IVR. DESIGN: Prospective clinical study...
April 2016: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
Miseon Shim, Do-Won Kim, Sunkyung Yoon, Gewnhi Park, Chang-Hwan Im, Seung-Hwan Lee
BACKGROUND: Deficits in facial emotion processing is a major characteristic of patients with panic disorder. It is known that visual stimuli with different spatial frequencies take distinct neural pathways. This study investigated facial emotion processing involving stimuli presented at broad, high, and low spatial frequencies in patients with panic disorder. METHODS: Eighteen patients with panic disorder and 19 healthy controls were recruited. Seven event-related potential (ERP) components: (P100, N170, early posterior negativity (EPN); vertex positive potential (VPP), N250, P300; and late positive potential (LPP)) were evaluated while the participants looked at fearful and neutral facial stimuli presented at three spatial frequencies...
June 2016: Journal of Affective Disorders
P Rossi, L Borghi, R Ferro, R Mencarelli
OBJECTIVE: The objectives of this study were to evaluate, in a selected HR-HPV positive population, the clinical performance of the p16/ki67 immunostaining in all the cytological diagnoses, as a reflex test of triage HPV-cytology, and assess the usefulness of p16/ki67-staining to classify CIN1 according to its risk of progression/regression in order to plan a personalized follow-up. METHODS: Our analysis was in consecutive cases of 1136 women aged 25-64 years, asymptomatic, HR-HPV DNA HC2 tested positive in a HPV-screening program, from February to December 2011...
September 2015: Pathologica
Haiyan Wu, Li Luo, Junqiang Dai, Suyong Yang, Naiyi Wang, Yue-Jia Luo
Research on familiar face recognition has largely focused on the neural correlates of recognizing a beloved partner or family member. However, no research has explored the effect of marriage style on the recognition of a beloved partner's face, especially in matriarchal societies. Here, we examined the time course of event-related potentials (ERP) in response to the face of a beloved partner, sibling, or unknown person in a sample of individuals from the matriarchal Mosuo tribe. Two groups were assessed: intermarriage and walking marriage groups (i...
2016: Frontiers in Psychology
Po-Fu Yang, Chih-Hung Wang, Gwo-Bin Lee
Cell fusion is a critical operation for numerous biomedical applications including cell reprogramming, hybridoma formation, cancer immunotherapy, and tissue regeneration. However, unstable cell contact and random cell pairings have limited efficiency and yields when utilizing traditional methods. Furthermore, it is challenging to selectively perform cell fusion within a group of cells. This study reports a new approach called optically-induced cell fusion (OICF), which integrates cell-pairing microstructures with an optically-induced, localized electrical field...
2016: Scientific Reports
Yu Ting Ma, Ce Wang, Xin Tao Yan, Zhi Hua Feng
A valve-type piezoelectric diaphragm pump using secondary resonant vibrator is introduced in this paper. The secondary resonant vibrator, which is mainly composed of a first vibrator and a second vibrator, is used to coordinate the frequency incompatibility between piezoelectric elements and check valves. The intermittent vibration of the first vibrator excites the resonant vibration of the second vibrator. The diaphragm in the pump chamber moves with the second vibrator, resulting in chamber volume and pressure variations...
January 2016: Review of Scientific Instruments
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