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https://www.readbyqxmd.com/read/28330329/diversity-of-bacteria-and-archaea-in-the-rhizosphere-of-bioenergy-crop-jatropha-curcas
#1
Garima Dubey, Bharati Kollah, Vijay Kumar Gour, Arvind Kumar Shukla, Santosh Ranjan Mohanty
Plant-microbial interaction in rhizosphere plays vital role in shaping plant's growth and ecosystem function. Most of the rhizospheric microbial diversity studies are restricted to bacteria. In natural ecosystem, archaea also constitutes a major component of the microbial population. However, their diversity is less known compared to bacteria. Experiments were carried out to examine diversity of bacteria and archaea in the rhizosphere of bioenergy crop Jatropha curcas (J. curcas). Samples were collected from three locations varying widely in the soil physico-chemical properties...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330327/role-of-gnb3-net-kcnj11-tcf7l2-and-grl-genes-single-nucleotide-polymorphism-in-the-risk-prediction-of-type-2-diabetes-mellitus
#2
REVIEW
Saliha Rizvi, Syed Tasleem Raza, Qamar Rahman, Farzana Mahdi
Type 2 diabetes (T2DM) is a polygenic metabolic disorder characterized by hyperglycemia occurring as a result of impaired insulin secretion or insulin resistance. Various environmental and genetic factors interact and increase the risk of T2DM and its complications. Among the various genetic factors associated with T2DM, single nucleotide polymorphism in different candidate genes have been studied intensively and the resulting genetic variants have been found to have either positive or negative association with T2DM thereby increasing or decreasing the risk of T2DM, respectively...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330319/roles-of-plant-hormones-and-anti-apoptosis-genes-during-drought-stress-in-rice-oryza-sativa-l
#3
Mohammad Ubaidillah, Fika Ayu Safitri, Jun-Hyeon Jo, Sang-Kyu Lee, Adil Hussain, Bong-Gyu Mun, Il Kyung Chung, Byung-Wook Yun, Kyung-Min Kim
We previously identified the rice (Oryza sativa) senescence-associated gene OsSAP which encodes a highly conserved protein involved in anti-apoptotic activity. This novel Bax suppressor-related gene regulates tolerance to multiple stresses in yeast. Here, we show the effects of drought stress on leaf and root tissues of plants over-expressing OsSAP in relation to the levels of phytohormones, abscisic acid (ABA), jasmonic acid (JA), indole-3-carboxylic acid (ICA), gibberellic acid (GA3), and zeatin. Results showed that rice plants over-expressing SAP were tolerant to drought stress compared to wild type and the plants over-expressing AtBI-1, which is a homolog of the human Bax inhibitor-1 in Arabidopsis...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330318/brevibacillus-borstelensis-and-streptomyces-albogriseolus-have-roles-to-play-in-degradation-of-herbicide-sulfosulfuron
#4
Ridhima Arya, Navnit Kumar Mishra, Anil K Sharma
Use of herbicides, in particular sulfosulfuron, at more than recommended doses has raised major concerns about the health hazards for animals and humans. In the present study, isolation of sulfosulfuron-degrading Brevibacillus borstelensis and Streptomyces albogriseolus from the field soils in the northwestern region of India was carried out where the use of sulfosulfuron is predominant, and further assessed for their potential to degrade sulfosulfuron individually and together in a consortium form under lab conditions...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330271/assessment-of-protein-silver-nanoparticles-toxicity-against-pathogenic-alternaria-solani
#5
Sobhy I I Abdel-Hafez, Nivien A Nafady, Ismail R Abdel-Rahim, Abeer M Shaltout, José-Antonio Daròs, Mohamed A Mohamed
Mycogenic synthesis of silver nanoparticles (AgNPs) was carried out in the present investigation using an aqueous extract of endophytic non-pathogenic Alternaria solani F10 (KT721914). The mycosynthesized AgNPs were characterized by means of spectroscopic and microscopic techniques. The surface plasmon resonance found at 430 nm confirmed the formation of stable AgNPs for several weeks at room temperature. Also, the results revealed the formation of spherical and monodispersed AgNPs with an average size of 14...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330270/expression-and-purification-of-a-gene-encoding-a-9-7%C3%A2-kda-pe-protein-of-mycobacterium-avium-subsp-paratuberculosis
#6
S Chandra Sekar, P P Goswami, R Deb
Mycobacterium avium subsp. paratuberculosis (Map) contains PE family antigens which are Proline and glutamic acid rich and may play important role as T-cell antigens. In the present study, the Map 1507 ORF encoding 9.7 kDa PE protein was amplified by polymerase chain reaction and cloned into E. coli vector pQE30 UA. The recombinant plasmid designated as pQ PE was transformed into E. coli M15 cells and induced with IPTG revealed the high level expression of 11.9 kDa His-fusion protein as estimated by migration in 15 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330265/isolation-and-characterization-of-plant-synergistic-bacteria-capable-of-degrading-xenobiotics-from-oil-spillage-sites
#7
Indranil De, Sarika Gupta
Oil spillage sites primarily contain various types of hydrocarbons, such as linear chain, polycyclic, and aromatic compounds, posing several detrimental effects on plants. Results from our previous study showed an alteration of various metabolomic parameters, indirectly resulting in an observable decline of growth in the mung seedlings upon incubation with phenol, toluene, xylene, and hexane. This study evaluates the role of these compounds upon plant growth and focusses to mitigate the effect of the same, using some isolated plant synergistic bacteria...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330249/emulsifying-activity-of-a-biosurfactant-produced-by-a-marine-bacterium
#8
K Abraham Peele, V Ravi Teja Ch, Vidya P Kodali
Biosurfactants produced by biofilm-forming bacteria have great applications in biotechnology, pharmaceutical, food engineering, bioremediation, and biohydrometallurgy industries. This study aimed to find out the bacteria that produce novel exopolymers (EPSs) which can find potential role in oil biodegradation. A screening procedure was performed to detect EPS-producing bacteria. The EPS producing isolate was identified as Acinetobacter species by 16S rDNA analysis. The polymer produced by the isolate has shown significant emulsification and surfactant activities, and the activities were compared to some of the commercial emulsifiers...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330216/potential-application-of-waste-from-castor-bean-ricinus-communis-l-for-production-for-xylanase-of-interest-in-the-industry
#9
Polyanna Nunes Herculano, Keila Aparecida Moreira, Raquel Pedrosa Bezerra, Tatiana Souza Porto, Cristina Maria de Souza-Motta, Ana Lúcia Figueiredo Porto
Xylanases activity (XY) from Aspergillus japonicus URM5620 produced by Solid-State Fermentation (SSF) of castor press cake (Ricinus communis) on different conditions of production and extraction by PEG/citrate aqueous two-phase system (ATPS) were investigated. XY production was influenced by substrate amount (5-10 g), initial moisture (15-35 %), pH (4.0-6.0) and temperature (25-35 °C), obtaining the maximum activity of 29,085 ± 1808 U g ds(-1) using 5.0 g of substrate with initial moisture of 15 % at 25 °C and pH 6...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330207/a-pmoa-based-study-reveals-dominance-of-yet-uncultured-type-i-methanotrophs-in-rhizospheres-of-an-organically-fertilized-rice-field-in-india
#10
Pranitha S Pandit, Dilip R Ranade, Prashant K Dhakephalkar, Monali C Rahalkar
Rice fields are one of the important sources of anthropogenic methane. Methanotrophs can oxidize up to 30 % of the produced methane and thus have a pivotal environmental role in methane mitigation. India occupies the largest region under rice cultivation; however, most of the studies done on methanotrophic communities have focused on the Northern region. We studied methanotrophic community of a flooded, organically fertilized rice field using pmoA clone library approach. Organic rice fields impose a more serious threat as they produce more methane...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330198/pretreatment-and-enzymatic-process-modification-strategies-to-improve-efficiency-of-sugar-production-from-sugarcane-bagasse
#11
Siddhartha Pal, Shereena Joy, Kalpana D Trimukhe, Pramod S Kumbhar, Anjani J Varma, Sasisanker Padmanabhan
Pretreatment and enzymatic hydrolysis play a critical role in the economic production of sugars and fuels from lignocellulosic biomass. In this study, we evaluated diverse pilot-scale pretreatments and different post-pretreatment strategies for the production of fermentable sugars from sugarcane bagasse. For the pretreatment of bagasse at pilot-scale level, steam explosion without catalyst and combination of sulfuric and oxalic acids at low and high loadings were used. Subsequently, to enhance the efficiency of enzymatic hydrolysis of the pretreated bagasse, three different post-pretreatment process schemes were investigated...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330190/phyto-synthesis-and-structural-characterization-of-catalytically-active-gold-nanoparticles-biosynthesized-using-delonix-regia-leaf-extract
#12
Preeti Dauthal, Mausumi Mukhopadhyay
Biological methods of nanoparticles synthesis are ecologically sound and sustainable alternative to the conventional methods. On the basis of aforesaid premise, the present study deals with the optimization and fabrication of gold nanoparticles (Au-NPs) using easily available bio-resource, Delonix regia leaf extract. The use of practically nontoxic natural extracts and water allows the synthesis pathways presented to be considered as ''green'' and so permitting the synthesized Au-NPs to be used in sensitive areas, such as bioremediation...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330185/agave-atrovirens-fibers-as-substrate-and-support-for-solid-state-fermentation-for-cellulase-production-by-trichoderma-asperellum
#13
Naivy Y Nava-Cruz, Juan C Contreras-Esquivel, Miguel A Aguilar-González, Alberto Nuncio, Raúl Rodríguez-Herrera, Cristóbal N Aguilar
Many efforts have been made to produce cellulase with better features and conditions, and filamentous fungi have played an important role in the bioprocess, growing in liquid and solid cultures with sugarcane bagasse, corn stover and others lignocellulosic materials. In the present study, Agave atrovirens fibers were partially characterized, thermal pretreated and used as support, substrate and inducer source for cellulolytic complex production by four strains of the genus Trichoderma, where T. asperellum was selected as the best option for this process after evaluating the enzyme activity and the invasion capacity on the pretreated Agave fibers...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330182/efficient-single-step-chromatographic-purification-of-recombinant-human-antithrombin-rhat-from-saccharomyces-cerevisiae
#14
Maheswara Reddy Mallu, Sandeep Vemula, Srinivasa Reddy Ronda
Antithrombin (AT) is a glycoprotein that inactivates the several physiological target enzymes of coagulation system. The effect of purification strategies plays a crucial role in getting maximum recovery of yield, purity and biological activity of recombinant human antithrombin (rhAT). In the present work, the task of purifying rhAT from Saccharomyces cerevisiae BY4741 has been carried out using two different approaches such as cross flow filtration (CFF) system and chromatography methods. In the first approach, the protein was concentrated and partially purified through CFF to achieve maximum recovery yield and purity of 87 and 94 %, respectively...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330180/biodegradation-of-propiconazole-by-newly-isolated-burkholderia-sp-strain-bbk_9
#15
Praveen Satapute, Basappa Kaliwal
The isolation of propiconazole (PCZ) degrading bacterium BBK_9 strain was done from paddy soil, and it was identified as Burkholderia sp. based on the morphological characteristics and biochemical properties combined with 16S rRNA gene sequencing analysis. It has been seen that the factors such as temperature and pH influence the biodegradation process. The role of plasmid was studied in the degradation process by plasmid curing method. The PCZ acts as the sole carbon source and as energy substrate which can be utilized by the strain for its growth in Mineral salt medium and degraded 8...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330176/molecular-dissection-of-pathway-components-unravel-atisine-biosynthesis-in-a-non-toxic-aconitum-species-a-heterophyllum-wall
#16
Varun Kumar, Nikhil Malhotra, Tarun Pal, Rajinder Singh Chauhan
Aconitum heterophyllum is an important component for various herbal drug formulations due to the occurrence of non-toxic aconites including marker compound, atisine. Despite huge pharmacological potential, the reprogramming of aconites production is limited due to lack of understanding on their biosynthesis. To address this problem, we have proposed here the complete atisine biosynthetic pathway for the first time connecting glycolysis, MVA/MEP, serine biosynthesis and diterpene biosynthetic pathways. The transcript profiling revealed phosphorylated pathway as a major contributor towards serine production in addition to repertoire of genes in glycolysis (G6PI, PFK, ALD and ENO), serine biosynthesis (PGDH and PSAT) and diterpene biosynthesis (KO and KH) sharing a similar pattern of expression (2-4-folds) in roots compared to shoots vis-à-vis atisine content (0-0...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330175/exploration-of-the-binding-modes-of-l-asparaginase-complexed-with-its-amino-acid-substrates-by-molecular-docking-dynamics-and-simulation
#17
Erva Rajeswara Reddy, Rajulapati Satish Babu, Potla Durthi Chandrasai, Pola Madhuri
Acute lymphocytic leukemia (ALL) is an outrageous disease worldwide. L-Asparagine (L-Asn) and L-glutamine (L-Gln) deamination plays crucial role in ALL treatment. Role of Erwinaze(®) (L-asparaginase from Erwinia chrysanthemi) in regulation of L-Asn and L-Gln has been confirmed by the experimental studies. Therapeutic research against ALL remained elusive with the lack of structural information on Erwinaze(®) enzyme. In this present study, homology model of the Erwinaze(®) was developed using MODELLER and the same was validated by various quality indexing tools...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330163/probing-an-optimal-class-distribution-for-enhancing-prediction-and-feature-characterization-of-plant-virus-encoded-rna-silencing-suppressors
#18
Abhigyan Nath, Karthikeyan Subbiah
To counter the host RNA silencing defense mechanism, many plant viruses encode RNA silencing suppressor proteins. These groups of proteins share very low sequence and structural similarities among them, which consequently hamper their annotation using sequence similarity-based search methods. Alternatively the machine learning-based methods can become a suitable choice, but the optimal performance through machine learning-based methods is being affected by various factors such as class imbalance, incomplete learning, selection of inappropriate features, etc...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330159/in-silico-characterization-and-differential-expression-pattern-analysis-of-conserved-hmg-coa-reductase-domain-isolated-from-aconitum-balfourii-stapf
#19
Eti Sharma, Saurabh Pandey, A K Gaur
The 3-hydroxy-3-methyl glutaryl CoA reductase (HMGR) is the key enzyme of mevalonate pathway in plants. A partial genomic DNA fragment encoding HMGR conserved domain (denoted as AbHMGR) is isolated from Aconitum balfourii Stapf. It comprises 871 bp encoding 290 amino acids. In silico analysis reveals that it had extensive similarities to other plant HMGR gene. Domain analysis of AbHMGR showed two highly conserved NADPH and HMG CoA domains. Docking study predicted inhibitor, substrate and cofactor binding sites in the protein...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330151/insight-into-thermophiles-and-their-wide-spectrum-applications
#20
REVIEW
Ridhi Mehta, Paavan Singhal, Hardeep Singh, Dhanashree Damle, Anil K Sharma
The deconstruction of biomass is a pivotal process for the manufacture of target products using microbial cells and their enzymes. But the enzymes that possess a significant role in the breakdown of biomass remain relatively unexplored. Thermophilic microorganisms are of special interest as a source of novel thermostable enzymes. Many thermophilic microorganisms possess properties suitable for biotechnological and commercial use. There is, indeed, a considerable demand for a new generation of stable enzymes that are able to withstand severe conditions in industrial processes by replacing or supplementing traditional chemical processes...
June 2016: 3 Biotech
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