keyword
MENU ▼
Read by QxMD icon Read
search

fluid physiology

keyword
https://www.readbyqxmd.com/read/28231273/periovulatory-follicular-fluid-levels-of-estradiol-trigger-inflammatory-and-dna-damage-responses-in-oviduct-epithelial-cells
#1
Sergio E Palma-Vera, Jennifer Schoen, Shuai Chen
OBJECTIVE: Ovarian steroid hormones (mainly E2 and P4) regulate oviduct physiology. Serum-E2 acts on the oviduct epithelium from the basolateral cell compartment. Upon ovulation, the apical compartment of the oviduct epithelium is temporarily exposed to follicular fluid, which contains much higher levels of E2 than serum. The aim of this study was to evaluate the effects of human periovulatory follicular fluid levels of E2 on oviduct epithelial cells using two porcine in vitro models...
2017: PloS One
https://www.readbyqxmd.com/read/28229250/role-of-mirnas-in-human-disease-and-inborn-errors-of-metabolism
#2
Ana Rivera-Barahona, Belén Pérez, Eva Richard, Lourdes R Desviat
MicroRNAs (miRNAs) are short, noncoding RNAs that regulate gene expression posttranscriptionally by base pairing with target messenger RNAs (mRNAs). They are estimated to target ∼60% of all human protein-coding genes and are involved in regulating key physiological processes and intracellular signaling pathways. They also exhibit tissue specificity, and their dysregulation is linked to the progression of pathology. Identifying disease associated miRNAs and their respective targets provides novel molecular insight into disease, enabling the design of new therapeutic strategies...
February 22, 2017: Journal of Inherited Metabolic Disease
https://www.readbyqxmd.com/read/28228375/is-it-time-to-reinvent-basic-cell-culture-medium
#3
Turney J McKee, Svetlana V Komarova
The success of in vitro experiments depends largely on the quality of the cell culture media. Four synthetic media formulations, Dulbecco's Modified Minimum Essential Media (DMEM), RPMI 1640, Minimum Essential Media and its alpha modification (MEM), and Medium 199 (M199) are used in ~90% of published in vitro studies. We compared the levels of electrolytes and carbohydrates in these media formulations to physiological values in plasma and cerebrospinal fluid. The most commonly used media, DMEM and RPMI 1640, largely deviate from physiological levels of glucose...
February 22, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28225091/phase-separation-in-ceramide-np-containing-lipid-model-membranes-neutron-diffraction-and-solid-state-nmr
#4
Annett Schroeter, Sören Stahlberg, Barbora Školová, Stefan Sonnenberger, Adina Eichner, Daniel Huster, Kateřina Vávrová, Thomas Hauß, Bodo Dobner, Reinhard H H Neubert, Alexander Vogel
The stratum corneum is the outermost layer of the skin and protects the organism against external influences as well as water loss. It consists of corneocytes embedded in a mixture of ceramides, fatty acids, and cholesterol in a molar ratio of roughly 1 : 1 : 1. The unique structural and compositional arrangement of these stratum corneum lipids is responsible for the skin barrier properties. Many studies investigated the organization of these barrier lipids and, in particular, the exact conformation of ceramides...
February 22, 2017: Soft Matter
https://www.readbyqxmd.com/read/28223196/conversion-to-purpurogallin-a-key-step-in-the-mechanism-of-the-potent-xanthine-oxidase-inhibitory-activity-of-pyrogallol
#5
Sari Honda, Yuya Fukuyama, Hisashi Nishiwaki, Akiko Masuda, Toshiya Masuda
In this study, the mechanism of the xanthine oxidase (XO) inhibitory activity of pyrogallol, the main inhibitor found in roasted coffee, was investigated. Pyrogallol was unstable and readily converted to purpurogallin in a pH 7.4 solution, a physiological model of human body fluids. The XO inhibitory activity of the produced purpurogallin was higher than that of pyrogallol, as evidenced by comparing their IC50 values (0.2 µmol L(-1) for purpurogallin, 1.6 µmol L(-1) for pyrogallol). The XO activity of pyrogallol was enhanced by pre-incubation in pH 7...
February 18, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28219399/noninvasive-detection-of-alarming-intracranial-pressure-changes-by-auditory-monitoring-in-early-management-of-brain-injury-a-prospective-invasive-versus-noninvasive-study
#6
Fabrice Giraudet, François Longeras, Aurélien Mulliez, Aurélie Thalamy, Bruno Pereira, Paul Avan, Laurent Sakka
BACKGROUND: In brain-injured patients intracranial pressure (ICP) is monitored invasively by a ventricular or intraparenchymal transducer. The procedure requires specific expertise and exposes the patient to complications such as malposition, hemorrhage or infection. As inner-ear fluid compartments are connected to the cerebrospinal fluid space, ICP changes elicit subtle changes in the physiology of the inner ear. Notably, we previously demonstrated that the phase of cochlear microphonic potential (CM) generated by sound stimuli rotates with ICP...
February 21, 2017: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/28219319/biomarkers-in-alzheimer-s-disease-recent-update
#7
Sushil Sharma, Walter Lipincott
Alzheimer disease (AD) is an age-related neurodegenerative disorder, characterized by loss of memory and cognitive function. It is the common cause of dementia in elderly and is a global health concern as the population of people aged 85 and older, is growing alarmingly. Although pharmacotherapy for the treatment of AD has improved, lot of work remains to treat this devastating disease. AD pathology begins even before the onset of clinical symptoms. Because therapies could be more effective if implemented early in the disease progression, it is highly prudent to discover reliable biomarkers, to detect its exact pathophysiology during pre-symptomatic stage...
February 20, 2017: Current Alzheimer Research
https://www.readbyqxmd.com/read/28218404/topical-medication-instillation-techniques-for-glaucoma
#8
REVIEW
Li Xu, Xuemei Wang, Meijing Wu
BACKGROUND: Glaucoma is a leading cause of irreversible blindness worldwide and the second most common cause of blindness after cataracts. The primary treatment for glaucoma aims to lower intraocular pressure (IOP) with the use of topical medicines. Topical medication instillation techniques, such as eyelid closure and nasolacrimal occlusion when instilling drops, have been proposed as potential methods to increase ocular absorption and decrease systemic absorption of the drops. OBJECTIVES: To investigate the effectiveness of topical medication instillation techniques compared with usual care or another method of instillation of topical medication in the management of glaucoma or ocular hypertension...
February 20, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28217778/a-critical-insight-into-the-development-pipeline-of-microfluidic-immunoassay-devices-for-the-sensitive-quantitation-of-protein-biomarkers-at-the-point-of-care
#9
REVIEW
Ana I Barbosa, Nuno M Reis
The latest clinical procedures for the timely and cost-effective diagnosis of chronic and acute clinical conditions, such as cardiovascular diseases, cancer, chronic respiratory diseases, diabetes or sepsis (i.e. the biggest causes of death worldwide), involve the quantitation of specific protein biomarkers released into the blood stream or other physiological fluids (e.g. urine or saliva). The clinical thresholds are usually in the femtomolar to picolomar range, and consequently the measurement of these protein biomarkers heavily relies on highly sophisticated, bulky and automated equipment in centralised pathology laboratories...
February 20, 2017: Analyst
https://www.readbyqxmd.com/read/28217013/contributions-to-the-study-of-blood-brain-flux-using-radioactive-tracers
#10
Ioan Mureşan, Ioan Cosma
In this paper, we present an original radiocirculographic method for investigates of cerebral blood flow, which has proven to be very useful, simple, and efficient for studies of brain hemodynamics. Physical considerations on injected radioactive tracer in cardiovascular system, allowed us to state a relationship for the blood flux, F, valued as the amount of fluid-blood that traverses a vascular segment in unit time. All these theoretical facts, along with a host of remarkable clinical results, are presented in a doctoral thesis entitled "The cerebral Hemodynamics in Essential Hypertension and Arteriosclerosis" of the eminent doctor Ioan Mures,an, who died in 1984, at only 50 years old...
January 2017: World Journal of Nuclear Medicine
https://www.readbyqxmd.com/read/28214513/molecular-dynamics-simulation-study-of-the-effect-of-glycerol-dialkyl-glycerol-tetraether-hydroxylation-on-membrane-thermostability
#11
Carme Huguet, Susanne Fietz, Antoni Rosell-Melé, Xavier Daura, Lionel Costenaro
Archaeal tetraether membrane lipids span the whole membrane width and present two C40 isoprenoid chains bound by two glycerol groups (or one glycerol and calditol). These lipids confer stability and maintain the membrane fluidity in mesophile to extremophile environments, making them very attractive for biotechnological applications. The isoprenoid lipid composition in archaeal membranes varies with temperature, which has placed these lipids in the focus of paleo-climatological studies for about a decade. Non-hydroxylated isoprenoid archaeal lipids are typically used as paleo-thermometry proxies, but recently identified hydroxylated (OH) derivatives have also been proposed as temperature proxies...
February 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28213251/on-line-monitoring-of-in-vitro-oral-bioaccessibility-tests-as-front-end-to-liquid-chromatography-for-determination-of-chlorogenic-acid-isomers-in-dietary-supplements
#12
Daniel Kremr, David J Cocovi-Solberg, Petra Bajerová, Karel Ventura, Manuel Miró
A novel fully automated in-vitro oral dissolution test assay as a front-end to liquid chromatography has been developed and validated for on-line chemical profiling and monitoring of temporal release profiles of three caffeoylquinic acid (CQA) isomers, namely, 3-CQA,4-CQA and 5-CQA, known as chlorogenic acids, in dietary supplements. Tangential-flow filtration is harnessed as a sample processing approach for on-line handling of CQA containing extracts of hard gelatin capsules and introduction of protein-free samples into the liquid chromatograph...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28212219/drug-infusion-systems-technologies-performance-and-pitfalls
#13
Uoo R Kim, Robert A Peterfreund, Mark A Lovich
This review aims to broadly describe drug infusion technologies and raise subtle but important issues arising from infusion therapy that can potentially lead to patient instability and morbidity. Advantages and disadvantages of gravity-dependent drug infusion are described and compared with electromechanical approaches for precise control of medication infusion, including large-volume peristaltic and syringe pumps. This review discusses how drugs and inert carriers interact within infusion systems and outlines several complexities and potential sources of drug error...
February 16, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28211543/in-vitro-and-in-vivo-physiology-of-low-nanomolar-concentrations-of-zn-2-in-artificial-cerebrospinal-fluid
#14
Haruna Tamano, Ryusuke Nishio, Yukina Shakushi, Miku Sasaki, Yuta Koike, Misa Osawa, Atsushi Takeda
Artificial cerebrospinal fluid (ACSF), i.e., brain extracellular medium, which includes Ca(2+) and Mg(2+), but not other divalent cations such as Zn(2+), has been used for in vitro and in vivo experiments. The present study deals with the physiological significance of extracellular Zn(2+) in ACSF. Spontaneous presynaptic activity is suppressed in the stratum lucidum of brain slices from young rats bathed in ACSF containing 10 nM ZnCl2, indicating that extracellular Zn(2+) modifies hippocampal presynaptic activity...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28210935/the-effect-of-fluid-resuscitation-on-the-effective-circulating-volume-in-patients-undergoing-liver-surgery-a-post-hoc-analysis-of-a-randomized-controlled-trial
#15
Jaap Jan Vos, A F Kalmar, H G D Hendriks, J Bakker, T W L Scheeren
To assess the significance of an analogue of the mean systemic filling pressure (Pmsa) and its derived variables, in providing a physiology based discrimination between responders and non-responders to fluid resuscitation during liver surgery. A post-hoc analysis of data from 30 patients undergoing major hepatic surgery was performed. Patients received 15 ml kg(-1) fluid in 30 min. Fluid responsiveness (FR) was defined as an increase of 20% or greater in cardiac index, measured by FloTrac-Vigileo(®). Dynamic preload variables (pulse pressure variation and stroke volume variation: PPV, SVV) were recorded additionally...
February 16, 2017: Journal of Clinical Monitoring and Computing
https://www.readbyqxmd.com/read/28210225/increased-apoptosis-in-the-paraventricular-nucleus-mediated-by-at1r-ras-erk1-2-signaling-results-in-sympathetic-hyperactivity-and-renovascular-hypertension-in-rats-after-kidney-injury
#16
Hongguo Zhu, Lishan Tan, Yumin Li, Jiawen Li, Minzi Qiu, Lanying Li, Mengbi Zhang, Min Liang, Aiqing Li
Background: The central nervous system plays a vital role in the development of hypertension, but the molecular regulatory mechanisms are not fully understood. This study aimed to explore signaling in the paraventricular nucleus (PVN) which might contribute to renal hypertension. Methods: Renal hypertension model was established by five-sixth nephrectomy operation (5/6Nx) in male Sprague Dawley rats. Ten weeks afterwards, they were random assigned to no treatment, or intracerebroventricular injection (ICV) with artificial cerebrospinal fluid, losartan [angiotensin II receptor type 1 (AT1R) antagonist], farnesylthiosalicylic acid (Ras inhibitor), PD98059 (MEK inhibitor), or SB203580 (p38 inhibitor) and Z-DEVD-FMK (caspase-3 inhibitor)...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28209740/quantitative-mri-volumetry-diffusivity-cerebrovascular-flow-and-cranial-hydrodynamics-during-head-down-tilt-and-hypercapnia-the-spacecot-study
#17
Larry Allen Kramer, Khader M Hasan, Ashot E Sargsyan, Karina Marshall-Goebel, Jörn Rittweger, Dorit Donoviel, Saki Higashi, Benson Mwangi, Darius A Gerlach, Eric M Bershad
PURPOSE: In order to improve the pathophysiologic understanding of visual changes observed in astronauts, we aimed to use quantitative MRI to measure anatomic and physiologic responses during a ground-based spaceflight analogue (head down tilt, HDT) combined with increased ambient carbon dioxide (CO2). METHODS: Six healthy, male subjects participated in the double-blinded, randomized cross-over design study with two conditions: 26.5 h of -12° HDT with ambient air and with 0...
February 16, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28209725/soluble-trem2-induces-inflammatory-responses-and-enhances-microglial-survival
#18
Li Zhong, Xiao-Fen Chen, Tingting Wang, Zhe Wang, Chunyan Liao, Zongqi Wang, Ruizhi Huang, Daxin Wang, Xinxiu Li, Linbei Wu, Lin Jia, Honghua Zheng, Meghan Painter, Yuka Atagi, Chia-Chen Liu, Yun-Wu Zhang, John D Fryer, Huaxi Xu, Guojun Bu
Triggering receptor expressed on myeloid cells 2 (TREM2) is an innate immune receptor expressed in microglia in the brain. A soluble form of TREM2 (sTREM2) derived from proteolytic cleavage of the cell surface receptor is increased in the preclinical stages of AD and positively correlates with the amounts of total and phosphorylated tau in the cerebrospinal fluid. However, the physiological and pathological functions of sTREM2 remain unknown. Here, we show that sTREM2 promotes microglial survival in a PI3K/Akt-dependent manner and stimulates the production of inflammatory cytokines depending on NF-κB...
February 16, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28208688/the-impact-of-heat-exposure-and-sleep-restriction-on-firefighters-work-performance-and-physiology-during-simulated-wildfire-suppression
#19
Grace E Vincent, Brad Aisbett, Brianna Larsen, Nicola D Ridgers, Rod Snow, Sally A Ferguson
This study was designed to examine the effects of ambient heat on firefighters' physical task performance, and physiological and perceptual responses when sleep restricted during simulated wildfire conditions. Thirty firefighters were randomly allocated to the sleep restricted (n = 17, SR; 19 °C, 4-h sleep opportunity) or hot and sleep restricted (n = 13, HOT + SR; 33 °C, 4-h sleep opportunity) condition. Firefighters performed two days of simulated, intermittent, self-paced work circuits comprising six firefighting tasks...
February 12, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28207920/paper-based-microfluidic-system-for-tear-electrolyte-analysis
#20
Ali K Yetisen, Nan Jiang, Ali Tamayol, Guillermo U Ruiz-Esparza, Yu Shrike Zhang, Sofía Medina-Pando, Aditi Gupta, James S Wolffsohn, Haider Butt, Ali Khademhosseini, Seok-Hyun Yun
The analysis of tear constituents at point-of-care settings has a potential for early diagnosis of ocular disorders such as dry eye disease, low-cost screening, and surveillance of at-risk subjects. However, current minimally-invasive rapid tear analysis systems for point-of-care settings have been limited to assessment of osmolarity or inflammatory markers and cannot differentiate between dry eye subclassifications. Here, we demonstrate a portable microfluidic system that allows quantitative analysis of electrolytes in the tear fluid that is suited for point-of-care settings...
February 16, 2017: Lab on a Chip
keyword
keyword
117001
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"