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https://www.readbyqxmd.com/read/29677589/generation-of-the-human-induced-pluripotent-stem-cell-hipsc-line-psmi002-a-from-a-patient-affected-by-the-jervell-and-lange-nielsen-syndrome-and-carrier-of-two-compound-heterozygous-mutations-on-the-kcnq1-gene
#1
Manuela Mura, Yee-Ki Lee, Monia Ginevrino, Rita Zappatore, Federica Pisano, Marina Boni, Federica Dagradi, Lia Crotti, Enza Maria Valente, Peter J Schwartz, Hung-Fat Tse, Massimiliano Gnecchi
We report the generation of human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a female patient carrier of the two compound heterozygous mutations c.568 C>T p.R190W (maternal allele), and c.1781 G>A p.R594Q (paternal allele) on the KCNQ1 gene, causing Jervell and Lange-Nielsen Syndrome (JLNS). To obtain hiPSCs, we used the classical approach of the four retroviruses each encoding for a reprogramming factor OCT4, SOX2, KLF4, cMYC. The obtained hiPSC clones display pluripotent stem cell characteristics, and differentiate into spontaneously beating cardiomyocytes (hiPSC-CMs)...
April 7, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29671115/utility-of-dna-rna-protein-and-functional-approaches-to-solve-cryptic-immunodeficiencies
#2
Margot A Cousin, Matthew J Smith, Ashley N Sigafoos, Jay J Jin, Marine I Murphree, Nicole J Boczek, Patrick R Blackburn, Gavin R Oliver, Ross A Aleff, Karl J Clark, Eric D Wieben, Avni Y Joshi, Pavel N Pichurin, Roshini S Abraham, Eric W Klee
PURPOSE: We report a female infant identified by newborn screening for severe combined immunodeficiencies (NBS SCID) with T cell lymphopenia (TCL). The patient had persistently elevated alpha-fetoprotein (AFP) with IgA deficiency, and elevated IgM. Gene sequencing for a SCID panel was uninformative. We sought to determine the cause of the immunodeficiency in this infant. METHODS: We performed whole-exome sequencing (WES) on the patient and parents to identify a genetic diagnosis...
April 18, 2018: Journal of Clinical Immunology
https://www.readbyqxmd.com/read/29669943/whole-exome-sequencing-uncovers-oxidoreductases-dhtkd1-and-ogdhl-as-linkers-between-mitochondrial-dysfunction-and-eosinophilic-esophagitis
#3
Joseph D Sherrill, Kiran Kc, Xinjian Wang, Ting Wen, Adam Chamberlin, Emily M Stucke, Margaret H Collins, J Pablo Abonia, Yanyan Peng, Qiang Wu, Philip E Putnam, Phillip J Dexheimer, Bruce J Aronow, Leah C Kottyan, Kenneth M Kaufman, John B Harley, Taosheng Huang, Marc E Rothenberg
Eosinophilic esophagitis (EoE) is an allergic inflammatory esophageal disorder with a complex underlying genetic etiology often associated with other comorbidities. Using whole-exome sequencing (WES) of 63 patients with EoE and 60 unaffected family members and family-based trio analysis, we sought to uncover rare coding variants. WES analysis identified 5 rare, damaging variants in dehydrogenase E1 and transketolase domain-containing 1 (DHTKD1). Rare variant burden analysis revealed an overabundance of putative, potentially damaging DHTKD1 mutations in EoE (P = 0...
April 19, 2018: JCI Insight
https://www.readbyqxmd.com/read/29669390/editorial-title-lessons-learned-from-a-failed-clinical-trial
#4
EDITORIAL
E F A Leijten, T R D J Radstake, K A Reedquist
Spleen tyrosine kinase (Syk), through its capacity to promote the pathogenic activation of B lymphocytes, myeloid cells, osteoclasts, and stromal fibroblast-like synoviocytes, has been subject to intense translational and clinical scrutiny as a potential therapeutic target in rheumatoid arthritis (RA) (1). Initial phase II clinical trials with the Syk inhibitor fostamatinib showed promising effectivity in treating RA patients that had displayed inadequate responses to methotrexate (2,3). However, concerns were raised of drug-dependent adverse effects in these studies, and fostamatinib did not improve disease in RA patients failing other biologic agents (4)...
April 18, 2018: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/29658303/-i-toxoplasma-gondii-i-infection-induces-the-formation-of-host-s-nuclear-granules-containing-poly-a-binding-proteins
#5
Karlee Fischer, Mikayla Roberts, Scott Roscoe, Yasin Avci, Sirinart Ananvoranich
To gain an insight into the mechanisms by which <i>Toxoplasma gondii</i> interacts with the host cells at the molecular level, human poly-(A) binding protein (PABP) is being investigated. PABP family is an indicator of translation in the cell, given its roles in mRNA stability, translational initiation and repression. Following a heat shock, the formation of PABP-containing granules was observed in both the cytoplasm and nucleus of human fibroblasts. This heat shock response does not affect the progression of <i>T...
April 15, 2018: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/29656749/tumor-associated-macrophages-support-the-growth-of-fgf9-induced-lung-adenocarcinoma-by-multiple-mechanisms
#6
Ahmed E Hegab, Mari Ozaki, Shizuko Kagawa, Junko Hamamoto, Hiroyuki Yasuda, Katsuhiko Naoki, Kenzo Soejima, Yongjun Yin, Tomonari Kinoshita, Tomonori Yaguchi, Yutaka Kawakami, David M Ornitz, Tomoko Betsuyaku
OBJECTIVES: Tumor-associated macrophages (TAMs) are known to promote tumorigenesis but the mechanism(s) remain elusive. We have developed a mouse model of lung cancer that is initiated through an inducible overexpression of fibroblast growth factor 9 (FGF9) in type-2 pneumocytes. Expression of FGF9 in adult lungs resulted in a rapid development of multiple adenocarcinoma-like tumor nodules, and is associated with an intense immunological reaction. The purpose of this study is to characterize the immune response to the FGF9-induced lung adenocarcinoma and to determine the contribution of TAMs to growth and survival of these tumors...
May 2018: Lung Cancer: Journal of the International Association for the Study of Lung Cancer
https://www.readbyqxmd.com/read/29653139/influence-of-zinc-oxide-quantum-dots-in-the-antibacterial-activity-and-cytotoxicity-of-an-experimental-adhesive-resin
#7
Isadora Martini Garcia, Vicente Castelo Branco Leitune, Fernanda Visioli, Susana Maria Werner Samuel, Fabrício Mezzomo Collares
OBJECTIVE: To evaluate the influence of zinc oxide quantum dots (ZnOQDs ) into an experimental adhesive resin regarding the antibacterial activity against Streptococcus mutans and the cytotoxicity against pulp fibroblasts. MATERIALS AND METHODS: ZnOQDs were synthesized by sol-gel process and were incorporated into 2-hydroxyethyl methacrylate (HEMA). An experimental adhesive resin was formulated by mixing 66.6 wt.% bisphenol A glycol dimethacrylate (BisGMA) and 33...
April 10, 2018: Journal of Dentistry
https://www.readbyqxmd.com/read/29651176/cloning-of-ompa-gene-from-acinetobacter-baumannii-into-the-eukaryotic-expression-vector-pbudce4-1-as-dna-vaccine
#8
Hossein Ansari, Abbas Doosti, Mohammad Kargar, Mahdi Bijanzadeh, Mojtaba Jaafarinia
Antibiotic resistant features of Acinetobacter baumannii is partly due to the decreased outer membrane proteins (OMPs) permeability. The OmpA is one of the most conserved proteins among A. baumannii with a considerable antigenic potential to stimulate the multidimensional immune system responses. The present study was aimed to clone the ompA gene into the eukaryotic expression vector with potential as DNA vaccine. The ompA gene of A. baumannii was amplified using polymerase chain reaction (PCR). The target DNA was cloned and sub-cloned into the pTZ57R/T and pBudCE4...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29650553/enduring-epigenetic-landmarks-define-the-cancer-microenvironment
#9
Ruth Pidsley, Mitchell G Lawrence, Elena Zotenko, Birunthi Niranjan, Aaron Statham, Jenny Song, Roman M Chabanon, Wenjia Qu, Hong Wang, Michelle Richards, Shalima S Nair, Nicola J Armstrong, Hieu T Nim, Melissa Papargiris, Preetika Balanathan, Hugh French, Timothy Peters, Sam Norden, Andrew Ryan, John Pedersen, James Kench, Roger J Daly, Lisa G Horvath, Phillip Stricker, Mark Frydenberg, Renea A Taylor, Clare Stirzaker, Gail P Risbridger, Susan J Clark
The growth and progression of solid tumors involves dynamic cross-talk between cancer epithelium and the surrounding microenvironment. To date, molecular profiling has largely been restricted to the epithelial component of tumors; therefore, features underpinning the persistent protumorigenic phenotype of the tumor microenvironment are unknown. Using whole-genome bisulfite sequencing, we show for the first time that cancer-associated fibroblasts (CAFs) from localized prostate cancer display remarkably distinct and enduring genome-wide changes in DNA methylation, significantly at enhancers and promoters, compared to nonmalignant prostate fibroblasts (NPFs)...
April 12, 2018: Genome Research
https://www.readbyqxmd.com/read/29649215/mobile-phone-specific-electromagnetic-fields-induce-transient-dna-damage-and-nucleotide-excision-repair-in-serum-deprived-human-glioblastoma-cells
#10
Halh Al-Serori, Franziska Ferk, Michael Kundi, Andrea Bileck, Christopher Gerner, Miroslav Mišík, Armen Nersesyan, Monika Waldherr, Manuel Murbach, Tamara T Lah, Christel Herold-Mende, Andrew R Collins, Siegfried Knasmüller
Some epidemiological studies indicate that the use of mobile phones causes cancer in humans (in particular glioblastomas). It is known that DNA damage plays a key role in malignant transformation; therefore, we investigated the impact of the UMTS signal which is widely used in mobile telecommunications, on DNA stability in ten different human cell lines (six brain derived cell lines, lymphocytes, fibroblasts, liver and buccal tissue derived cells) under conditions relevant for users (SAR 0.25 to 1.00 W/kg)...
2018: PloS One
https://www.readbyqxmd.com/read/29643956/serum-cytokines-in-early-prediction-of-anastomotic-leakage-following-low-anterior-resection
#11
Marek Zawadzki, Małgorzata Krzystek-Korpacka, Andrzej Gamian, Wojciech Witkiewicz
Introduction: Anastomotic leakage continues to be one of the most serious complications following low anterior resections. Early diagnosis of a leak is difficult but critical to minimize morbidity and mortality. Aim: To evaluate changes in serum concentrations of 27 different cytokines following low anterior resection, with the goal of finding new, early biomarkers of anastomotic leak. Material and methods: This is a prospective observational study that includes 32 patients undergoing elective low anterior resection for rectal cancer...
March 2018: Wideochirurgia i Inne Techniki Mało Inwazyjne, Videosurgery and Other Miniinvasive Techniques
https://www.readbyqxmd.com/read/29630097/effect-of-fgf2-on-the-activity-of-sprys-dusp6-erk-signaling-pathway-in-endometrial-glandular-epithelial-cells-of-endometriosis
#12
X-Y Yu, X-T Wang, Y-L Chu, H-M Qu, J Liu, Y-H Zhang, M-J Li
OBJECTIVE: We aimed at exploring the positive feedback loop in eutopic and ectopic endometrial glandular epithelial cells (EuECs and EECs) in endometriosis. MATERIALS AND METHODS: Normal epithelial cells (NECs), EuECs and EECs were treated with fibroblast growth factor (FGF)2, FGF2 neutralizing antibody, mitogen-activated protein kinases (MAPKs) inhibitors U0126 and PD98059. FGF2 protein level was detected by enzyme-linked immunosorbent assay (ELISA). The expressions of FGF2, FGF receptor 1 (FGFR1), extracellular signal-regulated kinase (ERK)1/2/pERK1/2 and Sproutys (SPRYs) (Sprouty1, Sprouty2, Sprouty4) and dual specificity phosphatase 6 (DUSP6) were detected by Western blot...
March 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29626695/multi-functional-electrospun-antibacterial-core-shell-nanofibrous-membranes-for-prolonged-prevention-of-post-surgical-tendon-adhesion-and-inflammation
#13
K T Shalumon, Chialin Sheu, Chih-Hao Chen, Shih-Heng Chen, Gils Jose, Chang-Yi Kuo, Jyh-Ping Chen
The possibility of endowing an electrospun anti-adhesive barrier membrane with multi-functionality, such as lubrication, prevention of fibroblast attachment and anti-infection and anti-inflammation properties, is highly desirable for the management of post-surgical tendon adhesion. To this end, we fabricated core-shell nanofibrous membranes (CSNMs) with embedded silver nanoparticles (Ag NPs) in the poly(ethylene glycol) (PEG)/poly(caprolactone) (PCL) shell and hyaluronic acid (HA)/ibuprofen in the core. HA imparted a lubrication effect for smooth tendon gliding and reduced fibroblast attachment, while Ag NPs and ibuprofen functioned as anti-infection and anti-inflammation agents, respectively...
April 4, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29625495/bevacizumab-promotes-t-cell-mediated-collagen-deposition-in-the-mouse-model-of-conjunctival-scarring
#14
Li-Fong Seet, Li Zhen Toh, Stephanie Chu, Sharon N Finger, Florent Ginhoux, Wanjin Hong, Tina T Wong
Purpose: We determine the effects of bevacizumab on collagen production in a mouse model of conjunctival scarring. Methods: Experimental surgery was performed as described for the mouse model of conjunctival scarring, and bevacizumab was introduced by conjunctival injection. The capacity of bevacizumab to recognize conjunctival VEGF-A was determined by ELISA. Col1a1 was measured by real-time PCR and immunoblotting. T cells and collagen were visualized by immunofluorescence and picrosirius red staining of bleb cryosections...
March 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29625013/successful-establishment-of-primary-type-2-alveolar-epithelium-with-3d-organotypic-co-culture
#15
Jennifer M S Sucre, Christopher S Jetter, Holli Loomans, Janice Williams, Erin J Plosa, John T Benjamin, Lisa R Young, Jonathan A Kropski, Carla L Calvi, Seunghyi Kook, Ping Wang, Linda Gleaves, Adel Eskaros, Laura Goetzl, Timothy S Blackwell, Susan H Guttentag, Andries Zijlstra
Alveolar type 2 (AT2) epithelial cells are uniquely specialized to produce surfactant in the lung and act as progenitor cells in the process of repair after lung injury. AT2 cell injury has been implicated in several lung diseases, including idiopathic pulmonary fibrosis and bronchopulmonary dysplasia. The inability to maintain primary AT2 cells in culture has been a significant barrier in the investigation of pulmonary biology. We have addressed this knowledge gap by developing a 3-dimensional organotypic co-culture using primary human fetal AT2 cells and pulmonary fibroblasts...
April 6, 2018: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/29621477/stromal-vascular-fraction-promotes-fibroblast-migration-and-cellular-viability-in-a-hyperglycemic-microenvironment-through-up-regulation-of-wound-healing-cytokines
#16
Shaun S Tan, Xin Yi Yeo, Zhen Chang Liang, Sunil K Sethi, Samuel S W Tay
Diabetic wounds have impaired healing and a propensity for further morbidity, which may result in amputations. Stromal vascular fraction (SVF) is an autologous source of heterogeneous cell population obtained from adipose tissue, which is rich in stem cells and presents little immunogenicity to the host. In this study, we hypothesized that murine fibroblasts subjected to hyperglycemic conditions co-treated with SVF exhibit greater functional activity through the colorimetric MTT assay and a cell-monolayer in-vitro scratch assay...
April 2, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29620451/flavonoids-from-plinia-cauliflora-mart-kausel-myrtaceae-with-antifungal-activity
#17
Tatiana M Souza-Moreira, Juliana A Severi, Edvânio Ramos Rodrigues, Mayara Ivani de Paula, Jolindo A Freitas, Wagner Vilegas, Rosemeire C L R Pietro
Plinia cauliflora is a plant species with edible fruits but its chemical composition is not totally known yet although former studies showed its potential as antifungal agent. This work aimed the chemical analysis of the leaves, the activity against fungi species and evaluated cytotoxicity. Extract was obtained with 70% ethanol. An ethyl acetate fraction was obtained and glycosylated quercetin and myricetin were isolated. Samples were tested against Candida species, dermatophytes and entomopathogenic fungi...
April 5, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29616048/tnfsf14-light-exhibits-inflammatory-activities-in-lung-fibroblasts-complementary-to-il-13-and-tgf-%C3%AE
#18
Ricardo da Silva Antunes, Amit K Mehta, Lisa Madge, Joel Tocker, Michael Croft
The cytokine TNFSF14 [homologous to Lymphotoxin, exhibits Inducible expression and competes with HSV Glycoprotein D for binding to HVEM, a receptor expressed on T lymphocytes (LIGHT)] has been shown in mouse models to be important for development of lung tissue remodeling that is characteristic of asthma, idiopathic pulmonary fibrosis (IPF), and systemic sclerosis (SSc). However, its cellular targets are not fully delineated. In the present report, we show that LTβR and HVEM, the receptors for LIGHT, are constitutively expressed in primary human lung fibroblasts (HLFs)...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29614500/precursor-t-lymphoblastic-lymphoma-associated-with-t-8-9-p11-2-q33-a-case-report-and-review-of-the-literature
#19
Hui Lv, Shaoyan Hu, Jun Lu, Qin Zhai, Zong Zhai, Zhizhuo Du, Yina Sun, Jie Li, Hailong He, Yi Wang
The 8p11 myeloproliferative syndrome (EMS) is an aggressive neoplasm associated with chromosomal translocations involving the fibroblast growth factor receptor 1 (FGFR1) tyrosine kinase gene on chromosome 8p11-12. A new case of a 9-year-old boy with leukocytosis, eosinophilia, and general lymphadenopathy is reported in this study. Bone marrow examination showed eosinophilic hyperplasia, with blast cells amounting to 6-7%. Karyotyping revealed cytogenetic abnormalities, including t(8;9)(p11.2;q3?3). Fluorescence in situ hybridization for the FGFR1 gene rearrangement yielded positive results...
April 4, 2018: Acta Haematologica
https://www.readbyqxmd.com/read/29611009/development-of-electrospun-chitosan-polyethylene-oxide-fibrinogen-biocomposite-for-potential-wound-healing-applications
#20
Tony T Yuan, Ann Marie DiGeorge Foushee, Monica C Johnson, Angela R Jockheck-Clark, Jonathan M Stahl
Normal wound healing is a highly complex process that requires the interplay of various growth factors and cell types. Despite advancements in biomaterials, only a few bioactive wound dressings reach the clinical setting. The purpose of this research was to explore the feasibility of electrospinning a novel nanofibrous chitosan (CS)-fibrinogen (Fb) scaffold capable of sustained release of platelet-derived growth factor (PDGF) for the promotion of fibroblast migration and wound healing. CS-Fb scaffolds were successfully electrospun using a dual-spinneret electrospinner and directly evaluated for their physical, chemical, and biological characteristics...
April 2, 2018: Nanoscale Research Letters
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