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https://www.readbyqxmd.com/read/29048704/hydroxyapatite-decreases-cytotoxicity-of-a-glass-ionomer-cement-by-calcium-fluoride-uptake-in-vitro
#1
Casiano Del Angel-Mosqueda, Rene Hernandez-Delgadillo, Osvelia E Rodríguez-Luis, María T Ramírez-Rodríguez, Silvia Munguía-Moreno, Norma V Zavala-Alonso, Juan M Solís-Soto, María A A Nakagoshi-Cepeda, Rosa I Sánchez-Nájera, Sergio E Nakagoshi-Cepeda, Claudio Cabral-Romero
BACKGROUND: Glass ionomer cements (GICs) are widely used in dentistry because of their remineralizing and cariostatic potential induced by fluoride. In vitro studies have reported cell toxicity triggered by GICs; however, the influence of hydroxyapatite (HAp) must be considered. The aim of this study was to evaluate the effect of HAp in decreasing the cytotoxicity of the GIC 3M Vitrebond in vitro. METHODS: Samples of 3M Vitrebond (powder, liquid and light-cured) were incubated in Dulbecco's modified Eagle's medium-Ham's F12 (DMEM-F12) for 24 hours at 37°C...
October 14, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/29047238/identification-of-10-candidate-biomarkers-distinguishing-tuberculous-and-malignant-pleural-fluid-by-proteomic-methods
#2
Chang Youl Lee, Ji Young Hong, Myung Goo Lee, In Bum Suh
PURPOSE: Pleural effusion, an accumulation of fluid in the pleural space, usually occurs in patients when the rate of fluid formation exceeds the rate of fluid removal. The differential diagnosis of tuberculous pleurisy and malignant pleural effusion is a difficult task in high tuberculous prevalence areas. The aim of the present study was to identify novel biomarkers for the diagnosis of pleural fluid using proteomics technology. MATERIALS AND METHODS: We used samples from five patients with transudative pleural effusions for internal standard, five patients with tuberculous pleurisy, and the same numbers of patients having malignant effusions were enrolled in the study...
November 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/29043413/cholecystokinin-receptor-antagonist-alters-pancreatic-cancer-microenvironment-and-increases-efficacy-of-immune-checkpoint-antibody-therapy-in-mice
#3
Jill P Smith, Shangzi Wang, Sandeep Nadella, Sandra A Jablonski, Louis M Weiner
Advanced pancreatic ductal adenocarcinoma (PDAC) has typically been resistant to chemotherapy and immunotherapy; therefore, novel strategies are needed to enhance therapeutic response. Cholecystokinin (CCK) has been shown to stimulate growth of pancreatic cancer. CCK receptors (CCKRs) are present on pancreatic cancer cells, fibroblasts, and lymphocytes. We hypothesized that CCKR blockade would improve response to immune checkpoint antibodies by promoting influx of tumor-infiltrating lymphocytes (TILs) and reducing fibrosis...
October 17, 2017: Cancer Immunology, Immunotherapy: CII
https://www.readbyqxmd.com/read/29038882/etelcalcetide-a-novel-calcimimetic-prevents-vascular-calcification-in-a-rat-model-of-renal-insufficiency-with-secondary-hyperparathyroidism
#4
Longchuan Yu, James E Tomlinson, Shawn T Alexander, Kelly Hensley, Chun-Ya Han, Denise Dwyer, Marina Stolina, Charles Dean, William G Goodman, William G Richards, Xiaodong Li
Etelcalcetide, a novel peptide agonist of the calcium-sensing receptor, prevents vascular calcification in a rat model of renal insufficiency with secondary hyperparathyroidism. Vascular calcification occurs frequently in patients with chronic kidney disease (CKD) and is a consequence of impaired mineral homeostasis and secondary hyperparathyroidism (SHPT). Etelcalcetide substantially lowers parathyroid hormone (PTH) and fibroblast growth factor-23 (FGF23) levels in SHPT patients on hemodialysis. This study compared the effects of etelcalcetide and paricalcitol on vascular calcification in rats with adenine-induced CKD and SHPT...
October 16, 2017: Calcified Tissue International
https://www.readbyqxmd.com/read/29038621/tweak-fn14-activation-participates-in-skin-inflammation
#5
REVIEW
Qilu Liu, Shengxiang Xiao, Yumin Xia
Tumor necrosis factor- (TNF-) like weak inducer of apoptosis (TWEAK) participates in multiple biological activities via binding to its sole receptor-fibroblast growth factor-inducible 14 (Fn14). The TWEAK/Fn14 signaling pathway is activated in skin inflammation and modulates the inflammatory responses of keratinocytes by activating nuclear factor-κB signals and enhancing the production of several cytokines, including interleukins, monocyte chemotactic protein-1, RANTES (regulated on activation, normal T cell expressed and secreted), and interferon gamma-induced protein 10...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/29037555/a-genome-wide-comparison-of-mesenchymal-stem-cells-derived-from-human-placenta-and-umbilical-cord
#6
Sen-Wen Teng, Yu-Shu Lo, Wei-Ting Liu, Yogi Hsuan, Willie Lin
OBJECTIVE: The human umbilical cord and placenta have been considered as attractive alternative sources for noninvasive isolation of human mesenchymal stem cells (hMSCs). Different sources of MSC may have individual differentiation potential and phenotype. In this study, we compared the genome-wide expression data of umbilical cord and placenta derived hMSCs to identify specific differential expression genes (DEGs) and corresponding functions. MATERIALS AND METHODS: We collected human placental tissues and umbilical cord from healthy full-term placenta (n = 17)...
October 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29036539/immune-cells-and-autoantibodies-in-pulmonary-arterial-hypertension
#7
Cheng Li, Pingping Liu, Rong Song, Yiqing Zhang, S Lei, Shangjie Wu
Analyses of immunity in pulmonary arterial hypertension (PAH) support the notion that maladaptation of the immune response exists. Altered immunity is an increasingly recognized feature of PAH. Indeed, a delicate balance between immunity and tolerance exists and any disturbance may result in chronic inflammation or autoimmunity. This is suggested by infiltration of various immune cells (e.g. macrophages, T and B lymphocytes) in remodeled pulmonary vessels. In addition, several types of autoantibodies directed against antinuclear antigens, endothelial cells (ECs) and fibroblasts have been found in idiopathic and systemic sclerosis-associated PAH...
September 27, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29035246/effect-of-extracts-of-the-endemic-plants-centaurea-lydia-and-phlomis-nissolii-on-toxoplasma-gondii
#8
Zeynep Özlem Doğan Şığva, Ezgi Eylül Hasvatan, Gizem Gülen, Remzi Uslu, Beyza Eryıldız, Cenk Durmuşkahya, Hüsniye Kayalar, Ahmet Özbilgin, Metin Korkmaz, Cumhur Gündüz
OBJECTIVE: This study aimed to investigate the potential antitoxoplasma activities of extracts of the endemic plants Centaurea lydia and Phlomis nissolii in a fibroblast cell culture infected with T. gondii trophozoites. METHODS: WI-38 cell lines treated with plant extracts (55 μg/mL each) and an untreated control were infected with 5×105 T. gondii trophozoites, and the number of parasites in the medium was determined on days 7, 14, and 24. RESULTS: No cytotoxic effects of C...
September 2017: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/29035086/notch-signaling-is-essential-for-maturation-self-renewal-and-tri-differentiation-of-in-vitro-derived-human-neural-stem-cells
#9
Katari Venkatesh, L Vinod Kumar Reddy, Salar Abbas, Madhubanti Mullick, Erfath Thanjeem Begum Moghal, Janardhana Papayya Balakrishna, Dwaipayan Sen
Although neural stem cells (NSCs) have potential applications in treating neurological disorders, much still needs to be understood about the differentiation biology for their successful clinical translation. In this study, we aimed at deriving NSCs from human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) and explored the role of Notch signaling in the differentiation process. The hUCB-MSCs were characterized as per guidelines of the International Society of Cellular Therapy. NSCs were successfully generated from hUCB-MSCs by using epidermal and fibroblast growth factors under serum-free conditions...
October 16, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/29034889/generation-of-a-pig-induced-pluripotent-stem-cell-pipsc-line-from-embryonic-fibroblasts-by-incorporating-lin28-to-the-four-transcriptional-factor-mediated-reprogramming-vsmui001-d
#10
Warunya Chakritbudsabong, Ladawan Sariya, Sirikron Pamonsupornvichit, Rassmeepen Pronarkngver, Somjit Chaiwattanarungruengpaisan, Joao N Ferreira, Piyathip Setthawong, Praopilas Phakdeedindan, Mongkol Techakumphu, Theerawat Tharasanit, Sasitorn Rungarunlert
Pig induced pluripotent stem cell (piPSC) line was generated from embryonic fibroblast cells using retroviral transduction approaches carrying human transcriptional factors: OCT4, SOX2, KLF4, c-MYC and LIN28. The generated piPSC line, VSMUi001-D, was positive for alkaline phosphatase activity and expressed the pluripotency associated transcription factors including OCT4, SOX2, NANOG and surface markers SSEA-1, all iPSC hallmarks of authenticity. Furthermore, VSMUi001-D exhibited a normal karyotype and formed embryoid bodies in vitro and teratomas in vivo...
October 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29034882/generation-of-human-induced-pluripotent-stem-cell-lines-from-human-dermal-fibroblasts-using-a-modified-rna-system
#11
Kyung-Ok Uhm, Gue Youn Go, So-Jung Kim, Eun Hee Jo, Hye Young Choi, Young Sam Im, Hye-Yeong Ha, Jung-Hyun Kim, Soo Kyung Koo
We generated human induced pluripotent stem cells (KSCBi002-B and KSCBi002-B-1) from the dermal fibroblasts of a donor using a modified RNA-based gene delivery method. According to GTG-banding analysis, the generated KSCBi002-B line has a cytogenetic abnormality (46,XY, t(1;4)(q21;q25)) that is distinct from that of the donor, whereas KSCBi002-B-1 has a normal karyotype (46,XY). These cell lines can be useful as a model for characterizing the hiPSCs generated by a non-viral and non-integrative system, or as a chromosomal balanced translocation model...
October 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29034589/involvement-of-local-renin-angiotensin-system-in-immunosuppression-of-tumor-microenvironment
#12
Kenta Nakamura, Tomonori Yaguchi, Gaku Ohmura, Asuka Kobayashi, Naoshi Kawamura, Takashi Iwata, Yukiko Kiniwa, Ryuhei Okuyama, Yutaka Kawakami
To improve the current cancer immunotherapies, strategies to modulate various immunosuppressive cells including myeloid derived suppressor cells (MDSCs) which were shown to be negative factors in the immune-checkpoint blockade therapy, need to be developed. In this study, we have evaluated the role of local renin-angiotensin system (RAS) in the tumor immune-microenvironment using murine models bearing tumor cell lines in which RAS was not involved in their proliferation and angiogenetic ability. Administration of angiotensin II receptor blockers (ARBs) to C57BL/6 mice bearing murine colon cancer cell line MC38 resulted in significant enhancement of tumor antigen gp70 specific T cells...
October 16, 2017: Cancer Science
https://www.readbyqxmd.com/read/29027526/-investigation-of-the-structure-of-a-functionally-intact-xenopericardial-bioconduit-after-long-term-implantation
#13
R A Mukhamadiyarov, N V Rutkovskaya, I V Milto, O D Sidopova, Yu А Kudryavtseva, L S Barbarash
AIM: to investigate the cellular composition of a functionally intact xenopericardial valve in a recipient with acquired mitral defect after long-term implantation. MATERIAL AND METHODS: A Uniline bioconduit (BC) ('Neocor', Kemerovo) removed from the heart in the mitral position at 7.2 years after implantation was investigated. Heart valve leaflets were fixed in a buffered 4% paraformaldehyde solution and imbedded in paraffin or epoxy resin. Slices made from the paraffin samples were stained with hematoxylin and eosin or underwent immunohistochemical (IHC) examination for typing endothelial cells, smooth muscle cells, macrophages, fibroblasts, and T and B lymphocytes...
2017: Arkhiv Patologii
https://www.readbyqxmd.com/read/29026003/neutralization-of-either-il-17a-or-il-17f-is-sufficient-to-inhibit-house-dust-mite-induced-allergic-asthma-in-mice
#14
Pauline Chenuet, Louis Fauconnier, Fahima Madouri, Tiffany Marchiol, Nathalie Rouxel, Aurélie Ledru, Pascal Mauny, Rachel Lory, Catherine Uttenhove, Jacques van Snick, Yoichiro Iwakura, Franco di Padova, Valérie Quesniaux, Dieudonnée Togbe, Bernhard Ryffel
T helper (Th)17 immune response participates in allergic lung inflammation and asthma is reduced in the absence of interleukin (IL)-17 in mice. Since IL-17A and IL-17F are induced and bind the shared receptor IL-17RA, we asked whether both IL-17A and IL-17F contribute to house dust mite (HDM) induced asthma. We report that allergic lung inflammation is attenuated in absence of either IL-17A or IL-17F with reduced airway hyperreactivity, eosinophilic inflammation, goblet cell hyperplasia, cytokine and chemokine production as found in absence of IL-17RA...
October 15, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/29024986/stromal-fibroblasts-from-perimenopausal-endometrium-exhibit-a-different-transcriptome-than-those-from-the-premenopausal-endometrium
#15
David W Erikson, Fatima Barragan, Terhi T Piltonen, Joseph C Chen, Shaina Balayan, Juan C Irwin, Linda C Giudice
Human endometrium undergoes extensive regeneration on a cyclic basis in premenopausal women and likely occurs through the contribution of stem/progenitor cells. Menopause results in the permanent cessation of menstrual cycles and is preceded by perimenopause, a period of several years in which endocrine and biological changes occur and is a period of risk for endometrial proliferative disorders. The objectives of this study were to identify mesenchymal stem cells (eMSC) and stromal fibroblasts (eSF) in endometrium of perimenopausal women and perform expression profile analysis of perimenopausal eMSC and eSF to gain insight into the biology of stem/progenitor and lineage cell populations during the transition to menopause...
August 19, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28990218/a-polysaccharide-from-dictyophora-indusiata-inhibits-the-immunosuppressive-function-of-cancer-associated-fibroblasts
#16
Shuyu Han, Chaoxiong Ma, Minghua Hu, Yuanyuan Wang, Fangli Ma, Ning Tao, Zhihai Qin
Reversing the function of cancer-associated fibroblasts (CAFs) may improve the efficacy of cancer therapy. Here, we isolated a novel polysaccharide from Dictyophora indusiata (ZSP4) and examined its effects on the function of prostate CAFs. The supernatant of prostate CAFs can stimulate the proliferation of immune cells and inhibit the growth of CD4+/CD8+ T cells. However, after ZSP4 stimulation, the functions of prostate CAFs were inhibited. The mechanism experiment shows that ZSP4 can stimulate prostate CAFs by down-regulating the expression of α-smooth muscle actin...
October 8, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28988012/healing-activity-of-proteolytic-fraction-p1g10-from-vasconcellea-cundinamarcensis-in-a-cutaneous-wound-excision-model
#17
K M Freitas, L S Barcelos, M V Caliari, C E Salas, M T P Lopes
The proteolytic enzymes from Vasconcellea cundinamarcensis have demonstrated efficacy to accelerate healing of skin lesions. We report here the efficacy of the proteolytic fraction - P1G10 during repair of excisional wounds in rodent model and analyze possible mediators involved. Using 0.05% P1G10 we observed on day 3rd increased wound contraction accompanied by an increase in activated neutrophils and VEGF relative to the control. On day 7th neutrophils returned to normal levels, and at 0.01% P1G10, an increase in NAG activity used to monitor monocyte/macrophage, was observed...
October 5, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28987475/current-perspectives-on-the-role-of-cd8-t-cells-in-systemic-sclerosis
#18
REVIEW
Patrizia Fuschiotti
Despite long-standing recognition of the importance of T cells in systemic sclerosis (SSc; scleroderma), the role of CD8(+) T cells in disease pathogenesis has not been well studied. Our work has shown that over-production of the pro-fibrotic cytokine IL-13 by peripheral blood effector/memory CD8(+) T cells is critical for predisposing patients to more severe forms of cutaneous fibrosis. Moreover, IL-13-producing CD8(+) T cells induce a pro-fibrotic phenotype in normal and SSc dermal fibroblasts, and exhibit a strong cytotoxic activity ex vivo...
October 5, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28982850/comparison-of-cold-atmospheric-plasma-devices-efficacy-on-osteosarcoma-and-fibroblastic-in-vitro-cell-models
#19
COMPARATIVE STUDY
Denis Gümbel, Bettina Suchy, Lasse Wien, Nadine Gelbrich, Matthias Napp, Axel Kramer, Axel Ekkernkamp, Georg Daeschlein, Matthias B Stope
BACKGROUND/AIM: Cold atmospheric plasma (CAP) attenuates tumor cell proliferation and induces apoptosis in various cell lines. While exerting marginal effects on non-neoplastic cells this unfolds promising applications in cancer therapy. The aim of the study was to analyse the effects of different CAP sources and application times on osteosarcoma (OS) cells and non-malignant fibroblast cell proliferation. MATERIALS AND METHODS: U2-OS and 3-T-3 fibroblasts were treated with three different approved medical devices...
October 2017: Anticancer Research
https://www.readbyqxmd.com/read/28982589/identification-of-a-novel-loss-of-function-phex-mutation-ala720ser-in-a-sporadic-case-of-adult-onset-hypophosphatemic-osteomalacia
#20
Katarzyna Goljanek-Whysall, Andreas Tridimas, Rachel McCormick, Nicki-Jayne Russell, Melissa Sloman, Alan Sorani, William D Fraser, Fadil M Hannan
Adults presenting with sporadic hypophosphatemia and elevations in circulating fibroblast growth factor-23 (FGF23) concentrations are usually investigated for an acquired disorder of FGF23 excess such as tumor induced osteomalacia (TIO). However, in some cases the underlying tumor is not detected, and such patients may harbor other causes of FGF23 excess. Indeed, coding-region and 3'UTR mutations of phosphate-regulating neutral endopeptidase (PHEX), which encodes a cell-surface protein that regulates circulating FGF23 concentrations, can lead to alterations in phosphate homeostasis, which are not detected until adulthood...
October 3, 2017: Bone
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