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https://www.readbyqxmd.com/read/28371463/microbial-cell-disruption-for-improving-lipid-recovery-using-pressurized-co2-role-of-co2-solubility-in-cell-suspension-sugar-broth-and-spent-media
#1
Md Shamim Howlader, William Todd French, Sara A Shields-Menard, Marta Amirsadeghi, Magan Green, Neeraj Rai
The study of in situ gas explosion to lyse the triglyceride-rich cells involves the solubilization of gas (e.g. carbon dioxide, CO2 ) in lipid rich cells under pressure followed by a rapid decompression, which allows the gas inside the cell to rapidly expand and rupture the cell from inside out. The aim of this study was to perform the cell disruption using pressurized CO2 as well as to determine the solubility of CO2 in Rhodotorula glutinis cell suspension, sugar broth media, and spent media. Cell disruption of R...
April 3, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28344645/mimicking-lichens-incorporation-of-yeast-strains-together-with-sucrose-secreting-cyanobacteria-improves-survival-growth-ros-removal-and-lipid-production-in-a-stable-mutualistic-co-culture-production-platform
#2
Tingting Li, Chien-Ting Li, Kirk Butler, Stephanie G Hays, Michael T Guarnieri, George A Oyler, Michael J Betenbaugh
BACKGROUND: The feasibility of heterotrophic-phototrophic symbioses was tested via pairing of yeast strains Cryptococcus curvatus, Rhodotorula glutinis, or Saccharomyces cerevisiae with a sucrose-secreting cyanobacterium Synechococcus elongatus. RESULTS: The phototroph S. elongatus showed no growth in standard BG-11 medium with yeast extract, but grew well in BG-11 medium alone or supplemented with yeast nitrogen base without amino acids (YNB w/o aa). Among three yeast species, C...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28341907/utilization-of-inulin-containing-waste-in-industrial-fermentations-to-produce-biofuels-and-bio-based-chemicals
#3
REVIEW
Stephen R Hughes, Nasib Qureshi, Juan Carlos López-Núñez, Marjorie A Jones, Joshua M Jarodsky, Luz Ángela Galindo-Leva, Mitchell R Lindquist
Inulins are polysaccharides that belong to an important class of carbohydrates known as fructans and are used by many plants as a means of storing energy. Inulins contain 20 to several thousand fructose units joined by β-2,1 glycosidic bonds, typically with a terminal glucose unit. Plants with high concentrations of inulin include: agave, asparagus, coffee, chicory, dahlia, dandelion, garlic, globe artichoke, Jerusalem artichoke, jicama, onion, wild yam, and yacón. To utilize inulin as its carbon and energy source directly, a microorganism requires an extracellular inulinase to hydrolyze the glycosidic bonds to release fermentable monosaccharides...
April 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28339535/fungemia-due-to-rare-opportunistic-yeasts-data-from-a-population-based-surveillance-in-spain
#4
Mario Fernández-Ruiz, Jesús Guinea, Mireia Puig-Asensio, Óscar Zaragoza, Benito Almirante, Manuel Cuenca-Estrella, José María Aguado
Fungemia due to rare yeasts constitutes an emerging but poorly investigated condition. Data on risk factors, clinical features, therapy, and outcome of episodes of fungemia due to rare (non-Candida, non-Cryptococcus) yeasts were analyzed in a population-based surveillance program conducted in 29 Spanish hospitals between May 2010 and April 2011. Species identification (DNA sequencing) and antifungal susceptibility testing (EUCAST and CLSI methods) were centrally performed. Fourteen out of 767 episodes of fungemia (1...
August 5, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28317762/evaluation-of-aroma-enhancement-for-ecolly-dry-white-wines-by-mixed-inoculation-of-selected-rhodotorula-mucilaginosa-and-saccharomyces-cerevisiae
#5
Xing-Chen Wang, Ai-Hua Li, Marta Dizy, Niamat Ullah, Wei-Xuan Sun, Yong-Sheng Tao
To improve the aroma profile of Ecolly dry white wine, the simultaneous and sequential inoculations of selected Rhodotorula mucilaginosa and Saccharomyces cerevisiae were performed in wine making of this work. The two yeasts were mixed in various ratios for making the mixed inoculum. The amount of volatiles and aroma characteristics were determined the following year. Mixed fermentation improved both the varietal and fermentative aroma compound composition, especially that of (Z)-3-hexene-1-ol, nerol oxide, certain acetates and ethyls group compounds...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28289902/discovery-of-synthesis-and-secretion-of-polyol-esters-of-fatty-acids-by-four-basidiomycetous-yeast-species-in-the-order-sporidiobolales
#6
Luis A Garay, Irnayuli R Sitepu, Tomas Cajka, Oliver Fiehn, Erin Cathcart, Russell W Fry, Atit Kanti, Agustinus Joko Nugroho, Sarah Asih Faulina, Sira Stephanandra, J Bruce German, Kyria L Boundy-Mills
Polyol esters of fatty acids (PEFA) are amphiphilic glycolipids produced by yeast that could play a role as natural, environmentally friendly biosurfactants. We recently reported discovery of a new PEFA-secreting yeast species, Rhodotorula babjevae, a basidiomycetous yeast to display this behavior, in addition to a few other Rhodotorula yeasts reported on the 1960s. Additional yeast species within the taxonomic order Sporidiobolales were screened for secreted glycolipid production. PEFA production equal or above 1 g L(-1) were detected in 19 out of 65 strains of yeast screened, belonging to 6 out of 30 yeast species tested...
March 13, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28275154/a-member-of-the-gut-mycobiota-modulates-host-purine-metabolism-exacerbating-colitis-in-mice
#7
Tyson R Chiaro, Ray Soto, W Zac Stephens, Jason L Kubinak, Charisse Petersen, Lasha Gogokhia, Rickesha Bell, Julio C Delgado, James Cox, Warren Voth, Jessica Brown, David J Stillman, Ryan M O'Connell, Anne E Tebo, June L Round
The commensal microbiota has an important impact on host health, which is only beginning to be elucidated. Despite the presence of fungal, archaeal, and viral members, most studies have focused solely on the bacterial microbiota. Antibodies against the yeast Saccharomyces cerevisiae are found in some patients with Crohn's disease (CD), suggesting that the mycobiota may contribute to disease severity. We report that S. cerevisiae exacerbated intestinal disease in a mouse model of colitis and increased gut barrier permeability...
March 8, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28272809/bioprospection-of-cold-adapted-yeasts-with-biotechnological-potential-from-antarctica
#8
María Martha Martorell, Lucas Adolfo Mauro Ruberto, Pablo Marcelo Fernández, Lucía Inés Castellanos de Figueroa, Walter Patricio Mac Cormack
The aim of this study was to investigate the ability to produce extracellular hydrolytic enzymes at low temperature of yeasts isolated from 25 de Mayo island, Antarctica, and to identify those exhibiting one or more of the evaluated enzymatic activities. A total of 105 yeast isolates were obtained from different samples and 66 were identified. They belonged to 12 basidiomycetous and four ascomycetous genera. Most of the isolates were ascribed to the genera Cryptococcus, Mrakia, Cystobasidium, Rhodotorula, Gueomyces, Phenoliferia, Leucosporidium, and Pichia...
March 8, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28253367/hg-tolerance-and-biouptake-of-an-isolated-pigmentation-yeast-rhodotorula-mucilaginosa
#9
Bing Liu, Chaogang Wang, Danxia Liu, Ning He, Xu Deng
A pigmented yeast R1 with strong tolerance to Hg2+ was isolated. Phylogenetic identification based on the analysis of 26S rDNA and ITS revealed R1 is a Rhodotorula mucilaginosa species. R1 was able to grow in the presence of 80 mg/L Hg2+, but the lag phase was much prolonged compared to its growth in the absence of Hg2+. The maximum Hg2+ binding capacity of R1 was 69.9 mg/g, and dead cells could bind 15% more Hg2+ than living cells. Presence of organic substances drastically reduced bioavailability of Hg2+ and subsequently decreased Hg2+ removal ratio from aqueous solution, but this adverse effect could be remarkably alleviated by the simultaneous process of cell propagation and Hg2+ biouptake with actively growing R1...
2017: PloS One
https://www.readbyqxmd.com/read/28242037/influence-of-high-power-ultrasound-on-selected-moulds-yeasts-and-alicyclobacillus-acidoterrestris-in-apple-cranberry-and-blueberry-juice-and-nectar
#10
Anet Režek Jambrak, Marina Šimunek, Silva Evačić, Ksenija Markov, Goran Smoljanić, Jadranka Frece
The purpose of this study was to investigate the effect of non-thermal technology, high power ultrasound (HPU) on inactivation of Aspergillus ochraceus 318, Penicillium expansum 565, Rhodotorula sp. 74, Saccharomyces cerevisiae 5 and Alicyclobacillus acidoterrestris DSM 3922 in clear juices and nectars from apple, blueberry and cranberry juice concentrate. Inoculated juice and nectars were treated by high power ultrasound (20kHz) according to procedure set by central composite design (CCD). Three operational parameters, amplitude (60, 90 and 120μm), temperature (20, 40 and 60°C), and treatment time (3, 6 or 9min) were varied in order to observe the influence of ultrasound and combination of ultrasound and slight heating (thermosonication) on growth and inactivation of selected microorganisms...
February 17, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28214272/fungal-monitoring-of-the-indoor-air-of-the-museo-de-la-plata-herbarium-argentina
#11
Andrea C Mallo, Lorena A Elíades, Daniela S Nitiu, Mario C N Saparrat
BACKGROUND: Biological agents, such as fungal spores in the air in places where scientific collections are stored, can attack and deteriorate them. AIMS: The aim of this study was to gather information on the indoor air quality of the Herbarium of Vascular Plants of the Museo de Ciencias Naturales de La Plata, Argentina, in relation to fungal propagules and inert particles. METHODS: This study was made using a volumetric system and two complementary sampling methods: (1) a non-viable method for direct evaluation, and (2) a viable method by culture for viable fungal propagules...
February 14, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28213136/systematic-development-of-a-two-stage-fed-batch-process-for-lipid-accumulation-in-rhodotorula-glutinis
#12
Eric Lorenz, Dennis Runge, Anna-Maria Marbà-Ardébol, Maximilian Schmacht, Ulf Stahl, Martin Senz
The application of oleaginous yeast cells as feed supplement, for instance in aqua culture, can be a meaningful alternative for fish meal and oil additives. Therefore, a two-stage fed-batch process split into growth and lipogenesis phase was systematically developed to enrich the oleaginous yeast Rhodotorula glutinis Rh-00301 with high amounts of lipids at industrial relevant biomasses. Thereby, the different carbon sources glucose, sucrose and glycerol were investigated concerning their abilities to serve as a suited raw material for growth and/or lipid accumulation...
March 20, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28168314/cocoa-butter-like-lipid-production-ability-of-non-oleaginous-and-oleaginous-yeasts-under-nitrogen-limited-culture-conditions
#13
Yongjun Wei, Verena Siewers, Jens Nielsen
Cocoa butter (CB) extracted from cocoa beans is the main raw material for chocolate production. However, growing chocolate demands and limited CB production has resulted in a shortage of CB supply. CB is mainly composed of three different kinds of triacylglycerols (TAGs), POP (C16:0-C18:1-C16:0), POS (C16:0-C18:1-C18:0), and SOS (C18:0-C18:1-C18:0). The storage lipids of yeasts, mainly TAGs, also contain relative high-level of C16 and C18 fatty acids and might be used as CB-like lipids (CBL). In this study, we cultivated six different yeasts, including one non-oleaginous yeast strain, Saccharomyces cerevisiae CEN...
February 6, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28155998/lipids-of-rhodotorula-mucilaginosa-iipl32-with-biodiesel-potential-oil-yield-fatty-acid-profile-fuel-properties
#14
Mahesh Khot, Debashish Ghosh
This study analyzes the single cell oil (SCO), fatty acid profile, and biodiesel fuel properties of the yeast Rhodotorula mucilaginosa IIPL32 grown on the pentose fraction of acid pre-treated sugarcane bagasse as a carbon source. The yeast biomass from nitrogen limiting culture conditions (15.3 g L(-1) ) was able to give the SCO yield of 0.17 g g(-1) of xylose consumed. Acid digestion, cryo-pulverization, direct in situ transesterification, and microwave assisted techniques were evaluated in comparison to the Soxhlet extraction for the total intracellular yeast lipid recovery...
April 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28144888/characterization-and-robust-nature-of-newly-isolated-oleaginous-marine-yeast-rhodosporidium-spp-from-coastal-water-of-northern-china
#15
Qiuzhen Wang, Yan Cui, Biswarup Sen, Wenmeng Ma, Rose Lynn Zheng, Xianhua Liu, Guangyi Wang
A total of ten marine yeast strains isolated from Bohai Sea, Northern China were identified to be members of three genera Rhodosporidium, Rhodotorula, and Cryptococcus. Two representative strains Rhodosporidium TJUWZ4 and Cryptococcus TJUWZA11 with high lipid content based on Nile red staining method were further characterized. A wide range of culture conditions (C and N sources, pH, temperature, salinity and C/N ratio) were tested to characterize the biomass and lipid production (yield and productivity) of these strains...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28030920/sandpits-as-a-reservoir-of-potentially-pathogenic-fungi-for-children
#16
Anna Wójcik, Joanna Błaszkowska, Piotr Kurnatowski, Katarzyna Góralska
INTRODUCTION AND OBJECTIVE: Fungi belonging to various physiological and morphological groups present in the environment are potential human pathogens. Some of them are considered as emerging pathogens. Therefore, their presence in children's playgrounds should be regarded as health risk factor. MATERIALS AND METHOD: Sixty-eight samples of sand collected from 17 sandpits of different localities in Łódź, Poland, in autumn 2010 and 2011, and in spring 2011 and 2012 were evaluated...
December 23, 2016: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/27940464/determination-of-fungal-community-diversity-in-fresh-and-traditional-chinese-fermented-pepper-by-pyrosequencing
#17
Lingyan Zhao, Yong Li, Liwen Jiang, Fangming Deng
Fermented pepper is one of the traditional Chinese fermented vegetables. The production mainly relies on the fermentation by natural microorganisms. This fermentation system is a unique and dynamic microecological environment, and involved microbial communities are very complex. In this study, 454 pyrosequencing was first used to investigate the fungal communities in fresh pepper and different fermentation phases. The results showed that fungal communities in fresh pepper (sample M_0) were more abundant than later fermented phases...
December 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27931081/presence-and-distribution-of-yeasts-in-the-reproductive-tract-of-healthy-female-horses
#18
A Azarvandi, A R Khosravi, H Shokri, M Talebkhan Garoussi, F Gharahgouzlou, G Vahedi, A Sharifzadeh
BACKGROUND: Yeasts are commensal organisms found in the reproductive and gastrointestinal tracts, and on the skin and other mucosa in mammals. OBJECTIVES: The purpose of this study was to isolate and identify the yeast flora in the caudal reproductive tract of healthy female horses. STUDY DESIGN: Longitudinal study. METHODS: A total of 453 samples were collected using double guarded swabs from the vestibule, clitoral fossa and vagina of 151 horses...
December 8, 2016: Equine Veterinary Journal
https://www.readbyqxmd.com/read/27895288/some-biological-activities-of-pigments-extracted-from-micrococcus-roseus-ptcc-1411-and-rhodotorula-glutinis-ptcc-5257
#19
Hossein Rostami, Hassan Hamedi, Mahmoud Yolmeh
The importance of replacing synthetic pigments with natural types is increasing day by day in the food industry due to the harmful effects of some synthetic pigments. Microorganisms are a major source of natural pigments, which nowadays have attracted the attention of researchers. In this study, carotenoid pigments were produced by Micrococcus roseus and Rhodotorula glutinis, and some of their biological properties such as antimicrobial, antioxidant, anticancer, and anti-inflammatory activities were evaluated...
December 2016: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/27887615/developing-a-set-of-strong-intronic-promoters-for-robust-metabolic-engineering-in-oleaginous-rhodotorula-rhodosporidium-yeast-species
#20
Yanbin Liu, Sihui Amy Yap, Chong Mei John Koh, Lianghui Ji
BACKGROUND: Red yeast species in the Rhodotorula/Rhodosporidium genus are outstanding producers of triacylglyceride and cell biomass. Metabolic engineering is expected to further enhance the productivity and versatility of these hosts for the production of biobased chemicals and fuels. Promoters with strong activity during oil-accumulation stage are critical tools for metabolic engineering of these oleaginous yeasts. RESULTS: The upstream DNA sequences of 6 genes involved in lipid biosynthesis or accumulation in Rhodotorula toruloides were studied by luciferase reporter assay...
November 25, 2016: Microbial Cell Factories
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