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Sara Landolfo, Giuseppe Ianiri, Salvatore Camiolo, Andrea Porceddu, Giuliana Mulas, Rossella Chessa, Giacomo Zara, Ilaria Mannazzu
A molecular approach was applied to the study of the carotenoid biosynthetic pathway of Rhodotorula mucilaginosa. At first, functional annotation of the genome of R. mucilaginosa C2.5t1 was carried out and gene ontology categories were assigned to 4033 predicted proteins. Then, a set of genes involved in different steps of carotenogenesis was identified and those coding for phytoene desaturase, phytoene synthase/lycopene cyclase and carotenoid dioxygenase (CAR genes) proved to be clustered within a region of ~10 kb...
December 8, 2017: Microbiology
Raquel Quintilla, Anna Kolecka, Serge Casaregola, Heide M Daniel, Jos Houbraken, Markus Kostrzewa, Teun Boekhout, Marizeth Groenewald
Since food spoilage by yeasts causes high economic losses, fast and accurate identifications of yeasts associated with food and food-related products are important for the food industry. In this study the efficiency of the matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify food related yeasts was evaluated. A CBS in-house MALDI-TOF MS database was created and later challenged with a blinded test set of 146 yeast strains obtained from food and food related products...
November 24, 2017: International Journal of Food Microbiology
Yiqing Liu, Lin Wu, Xingwen Wu, Honghai Li, Qinhong Liao, Xiaojing Zhang, Zhiqiang Sun, Wenhua Li
Ginger is a perennial monocotyledonous herb, which can be used as both a vegetable and a medicinal plant. However, it is susceptible to various plant pathogens. Microbial diversity in soil is related closely to the health and productivity of plant crops including ginger. In the current study, we compared microbial diversity from soil samples under ginger cultivation (disease incidence of >50% [relatively unhealthy sample] versus disease incidence of <10% [relatively healthy sample]). The bacterial and fungal taxa were analyzed by Illumina-based sequencing, with 16S and ITS identification, respectively...
2017: Scientifica
Małgorzata J Biegańska, Magdalena Rzewuska, Iwona Dąbrowska, Bożena Malewska-Biel, Magdalena Ostrzeszewicz, Bożena Dworecka-Kaszak
This report describes the isolation of two environmental fungi: Rhodotorula mucilaginosa and Trichosporon jirovecii accompanied by Pseudomonas aeruginosa and Escherichia coli from a dog with bronchotracheitis. All microorganisms were isolated routinely from a mucopurulent discharge, obtained during bronchoscopy from laryngotracheal area. The initial identification of yeasts was confirmed by API Candida and by molecular analysis of internal transcribed spacer region. Administered antimicrobial treatment with Marbofloxacin and Canizol has brought the improvement in the dogs' health status...
December 1, 2017: Mycopathologia
M Barra, A Llanos-Rivera, F Cruzat, N Pino-Maureira, R R González-Saldía
Fish oil is used in the production of feed for cultured fish owing to its high polyunsaturated fatty acid content (PUFA). The over-exploitation of fisheries and events like "El Niño" are reducing the fish oil supply. Some marine microorganisms are considered potentially as alternative fatty acid sources. This study assesses a strain of Rhodotorula sp. (strain CNYC4007; 27% docosahexaenoic acid (DHA) of total fatty acids), as feed for fish larvae. The total length and ribonucleic acid (RNA)/deoxyribonucleic acid (DNA) ratio of Danio rerio larvae was determined at first feeding at six and 12 days old (post-yolk absorption larvae)...
December 1, 2017: Marine Drugs
Han Ming Gan, Bolaji N Thomas, Nicole T Cavanaugh, Grace H Morales, Ashley N Mayers, Michael A Savka, André O Hudson
In industry, the yeast Rhodotorula mucilaginosa is commonly used for the production of carotenoids. The production of carotenoids is important because they are used as natural colorants in food and some carotenoids are precursors of retinol (vitamin A). However, the identification and molecular characterization of the carotenoid pathway/s in species belonging to the genus Rhodotorula is scarce due to the lack of genomic information thus potentially impeding effective metabolic engineering of these yeast strains for improved carotenoid production...
2017: PeerJ
Luciana P Prendes, María G Merín, Ariel R Fontana, Rubén A Bottini, María L Ramirez, Vilma I Morata de Ambrosini
Epiphytic isolates with yeast characteristics from grapes of the Malbec cultivar were obtained in order to find antagonists against Alternaria alternata. From a total of 111 isolates, 82% corresponded to the yeast-like organism Aureobasidium pullulans and the rest to the non-Saccharomyces yeasts Hanseniaspora uvarum (6.3%), Metschnikowia pulcherrima or spp. (5.4%), Cryptoccocus laurentti II (2.7%), Starmerella bacilaris or Candida zemplinina (2.7%) and Rhodotorula spp. (0.9%). The 22.4% (15 out of 67) of epiphytic yeasts and yeast-like organisms evaluated were able to reduce A...
November 8, 2017: International Journal of Food Microbiology
P W Crous, M J Wingfield, T I Burgess, G E St J Hardy, P A Barber, P Alvarado, C W Barnes, P K Buchanan, M Heykoop, G Moreno, R Thangavel, S van der Spuy, A Barili, S Barrett, S O Cacciola, J F Cano-Lira, C Crane, C Decock, T B Gibertoni, J Guarro, M Guevara-Suarez, V Hubka, M Kolařík, C R S Lira, M E Ordoñez, M Padamsee, L Ryvarden, A M Soares, A M Stchigel, D A Sutton, A Vizzini, B S Weir, K Acharya, F Aloi, I G Baseia, R A Blanchette, J J Bordallo, Z Bratek, T Butler, J Cano-Canals, J R Carlavilla, J Chander, R Cheewangkoon, R H S F Cruz, M da Silva, A K Dutta, E Ercole, V Escobio, F Esteve-Raventós, J A Flores, J Gené, J S Góis, L Haines, B W Held, M Horta Jung, K Hosaka, T Jung, Ž Jurjević, V Kautman, I Kautmanova, A A Kiyashko, M Kozanek, A Kubátová, M Lafourcade, F La Spada, K P D Latha, H Madrid, E F Malysheva, P Manimohan, J L Manjón, M P Martín, M Mata, Z Merényi, A Morte, I Nagy, A-C Normand, S Paloi, N Pattison, J Pawłowska, O L Pereira, M E Petterson, B Picillo, K N A Raj, A Roberts, A Rodríguez, F J Rodríguez-Campo, M Romański, M Ruszkiewicz-Michalska, B Scanu, L Schena, M Semelbauer, R Sharma, Y S Shouche, V Silva, M Staniaszek-Kik, J B Stielow, C Tapia, P W J Taylor, M Toome-Heller, J M C Vabeikhokhei, A D van Diepeningen, N Van Hoa, Van Tri M, N P Wiederhold, M Wrzosek, J Zothanzama, J Z Groenewald
Novel species of fungi described in this study include those from various countries as follows: Australia: Banksiophoma australiensis (incl. Banksiophoma gen. nov.) on Banksia coccinea, Davidiellomycesaustraliensis (incl. Davidiellomyces gen. nov.) on Cyperaceae, Didymocyrtis banksiae on Banksia sessilis var. cygnorum, Disculoides calophyllae on Corymbia calophylla, Harknessia banksiae on Banksia sessilis, Harknessia banksiae-repens on Banksia repens, Harknessia banksiigena on Banksia sessilis var. cygnorum, Harknessia communis on Podocarpus sp...
June 2017: Persoonia
Iglė Vepštaitė-Monstavičė, Juliana Lukša, Ramunė Stanevičienė, Živilė Strazdaitė-Žielienė, Vyacheslav Yurchenko, Saulius Serva, Elena Servienė
The microbial assemblies on the surface of plants correlate with specific climatic features, suggesting a direct link between environmental conditions and microbial inhabitation patterns. At the same time however, microbial communities demonstrate distinct profiles depending on the plant species and region of origin. In this study, we report Next Generation Sequencing-based metagenomic analysis of microbial communities associated with apple and blackcurrant fruits harvested from Lithuania and the Czech Republic...
January 2018: Microbiological Research
Young-Kyoung Park, Jean-Marc Nicaud, Rodrigo Ledesma-Amaro
Moving our society towards a bioeconomy requires efficient and sustainable microbial production of chemicals and fuels. Rhodotorula (Rhodosporidium) toruloides is a yeast that naturally synthesizes substantial amounts of specialty chemicals and has been recently engineered to (i) enhance its natural production of lipids and carotenoids, and (ii) produce novel industrially relevant compounds. The use of R. toruloides by companies and research groups has exponentially increased in recent years as a result of recent improvements in genetic engineering techniques and the availability of multiomics information on its genome and metabolism...
November 10, 2017: Trends in Biotechnology
Jordana R P de Sousa, Vívian N Gonçalves, Rodrigo A de Holanda, Daniel A Santos, Cinthia F L G Bueloni, Adriana O Costa, Maria V Petry, Carlos A Rosa, Luiz H Rosa
We assessed the diversity of cultivable fungi in the ornithogenic soil nests of bird species like Phalacrocorax atriceps, Macronectes giganteus, Pygoscelis antarcticus, and Pygoscelis papua in the Antarctic islands. From 481 fungi isolated at 15 °C, only 50 displayed growth at 37 °C, and were identified as 14 species of 15 genera. Aspergillus fumigatus, Penicillium chrysogenum, and Rhodotorula mucilaginosa were the most abundant species obtained. Fifty taxa grew at 40 °C; displayed haemolytic and phospholipase activities; produced tiny spores, capsule, and melanin; showed growth at different pH; and showed resistance to amphotericin B...
December 2017: Fungal Biology
R D Pacheco-Cano, R Salcedo-Hernández, J E López-Meza, D K Bideshi, J E Barboza-Corona
AIMS: The objective of this study was to show whether the edible part of broccoli has antibacterial and antifungal activity against microorganism of importance in human health and vegetable spoilage, and to test if this effect was partially due to antimicrobial peptides. METHODS AND RESULTS: Crude extracts were obtained from florets and stems of broccoli cultivar Avenger and the inhibitory effect was demonstrated against pathogenic bacteria (Bacillus cereus, Staphylococcus xylosus, S...
November 7, 2017: Journal of Applied Microbiology
Krzysztof Szafrański, Jarosław Sławiński, Anna Kędzia, Ewa Kwapisz
Candidiasis represent a serious threat for patients with altered immune responses. Therefore, we have undertaken the synthesis of compounds comprising a pyridine-3-sulfonamide scaffold and known antifungally active 1,2,4-triazole substituents. Thus a series of novel 4-substituted N-(5-amino-1H-1,2,4-triazol-3-yl)pyridine-3-sulfonamides have been synthesized by multistep reactions starting from 4-chloropyridine-3-sulfonamide via N'-cyano-N-[(4-substitutedpyridin-3-yl)sulfonyl]carbamimidothioates which were further converted with hydrazine hydrate to the corresponding 1,2,4-triazole derivatives 26-36...
November 7, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Sangdon Ryu, Mi Ri Park, Brighton E Maburutse, Su Jin Lee, Woong Ji Lee, Soohyun Cho, Inho Hwang, Sangnam Oh, Younghoon Kim
Beef was dry aged for 40 to 60 days under controlled environmental conditions in a refrigerated room with a relative humidity of 75% to 80 % and air-flow. To date, there is little information on the microbial diversity and characteristics of dry aged beef. Here, we explored the effect of change in meat microorganisms on dry aged beef. Initially, total bacteria and LAB were significantly increased for 50 days during all dry aging periods. There was absence of representative foodborne pathogens as well as coliforms...
October 30, 2017: Journal of Microbiology and Biotechnology
Jia Wu, Adam Elliston, Gwenaelle Le Gall, Ian J Colquhoun, Samuel R A Collins, Jo Dicks, Ian N Roberts, Keith W Waldron
In addition to ethanol, yeasts have the potential to produce many other industrially-relevant chemicals from numerous different carbon sources. However there remains a paucity of information about overall capability across the yeast family tree. Here, 11 diverse species of yeasts with genetic backgrounds representative of different branches of the family tree were investigated. They were compared for their abilities to grow on a range of sugar carbon sources, to produce potential platform chemicals from such substrates and to ferment hydrothermally pretreated rice straw under simultaneous saccharification and fermentation conditions...
October 27, 2017: Scientific Reports
Dorota Kregiel, Steve A James, Anna Rygala, Joanna Berlowska, Hubert Antolak, Ewelina Pawlikowska
Yeast strains and acetic acid bacteria were isolated from spoiled soft drinks with characteristic flocs as a visual defect. Polymerase chain reaction and amplification of a partial region of the LSU rRNA gene identified the bacteria as Asaia spp. Sequence analysis of the D1/D2 region of the 26S rDNA in turn identified the yeast isolates as Wickerhamomyces anomalus, Dekkera bruxellensis and Rhodotorula mucilaginosa. The hydrophobicity and adhesion properties of the yeasts were evaluated in various culture media, taking into account the availability of nutrients and the carbon sources...
October 20, 2017: Antonie Van Leeuwenhoek
Xiangfeng Zheng, Qiya Yang, Lina Zhao, Maurice Tibiru Apaliya, Xiaoyun Zhang, Hongyin Zhang
The proteomic and lysine acetylation (Kac) changes, accompanying degradation of patulin in Rhodotorula mucilaginosa were analyzed using tandem mass tagging and N6-acetyllysine affinity enrichment followed by LC-MS/MS. Proteomic results showed that expression level of short-chain reductase protein and glutathione S-transferase involved in detoxification was significantly up-regulated. In addition, the expression levels of zinc-binding oxidoreductase and quinone oxidoreductase that are involved in antioxidant process, ABC transport and MFS transport responsible for chemical transport were activated when treated with patulin...
October 18, 2017: Scientific Reports
Marie Horká, Karel Šlais, Jiří Šalplachta, Filip Růžička
Pre-separation and pre-concentration of bacteria is an important step especially when they are uncultured and bacterial concentration in the matrix is low. This study describes a preparative method based on isoelectric focusing of colored microorganisms in a cellulose-based separation medium from a high conductivity matrix. The isoelectric points found for the examined cells were 1.8 for Micrococcus luteus, 3.5 for Dietzia sp., and 4.7 for Rhodotorula mucilaginosa using capillary isoelectric focusing. The final positions of the zones of colored microbial cells in the cellulose-bed are indicated by colored pI markers...
October 16, 2017: Analytica Chimica Acta
Ines Lačanin, Jérôme Mounier, Audrey Pawtowski, Marta Dušková, Josef Kameník, Renáta Karpíšková
Fungi are commonly involved in dairy product spoilage and the use of bioprotective cultures can be a complementary approach to reduce food waste and economic losses. In this study, the antifungal activity of 89 Lactobacillus and 23 Pediococcus spp. isolates against three spoilage species, e.g., Yarrowia lipolytica, Rhodotorula mucilaginosa and Penicillium brevicompactum, was first evaluated in milk agar. None of the tested pediococci showed antifungal activity while 3, 23 and 43 lactobacilli isolates showed strong antifungal activity or total inhibition against Y...
September 30, 2017: World Journal of Microbiology & Biotechnology
Valentina V Ebani, Simona Nardoni, Fabrizio Bertelloni, Basma Najar, Luisa Pistelli, Francesca Mancianti
Background: Essential oils (EOs) are recommended by some veterinarians to treat otitis externa in pets, but data about their efficacy in scientific literature are very scant. Methods: Nine commercial EOs, from roman chamomile (Anthemis nobilis L.), star anise (Illicium verum), lavender (Lavandula hybrida), litsea (Litsea cubeba (Lour.) Pers.), basil (Ocimum basilicum L.), oregano (Origanum vulgare L. subsp. hirticum), rosemary (Rosmarinus officinalis L.), clary sage (Salvia sclarea L.), and thyme (Thymus vulgaris L...
April 21, 2017: Medicines (Basel, Switzerland)
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