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https://www.readbyqxmd.com/read/29029742/preparative-isoelectric-focusing-of-microorganisms-in-cellulose-based-separation-medium-and-subsequent-analysis-by-cief-and-maldi-tof-ms
#1
Marie Horká, Karel Šlais, Jiří Šalplachta, Filip Růžička
Pre-separation and pre-concentration of bacteria is an important step especially when they are uncultured and bacterial concentration in the matrix is low. This study describes a preparative method based on isoelectric focusing of colored microorganisms in a cellulose-based separation medium from a high conductivity matrix. The isoelectric points found for the examined cells were 1.8 for Micrococcus luteus, 3.5 for Dietzia sp., and 4.7 for Rhodotorula mucilaginosa using capillary isoelectric focusing. The final positions of the zones of colored microbial cells in the cellulose-bed are indicated by colored pI markers...
October 16, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28965275/assessment-of-the-antifungal-activity-of-lactobacillus-and-pediococcus-spp-for-use-as-bioprotective-cultures-in-dairy-products
#2
Ines Lačanin, Jérôme Mounier, Audrey Pawtowski, Marta Dušková, Josef Kameník, Renáta Karpíšková
Fungi are commonly involved in dairy product spoilage and the use of bioprotective cultures can be a complementary approach to reduce food waste and economic losses. In this study, the antifungal activity of 89 Lactobacillus and 23 Pediococcus spp. isolates against three spoilage species, e.g., Yarrowia lipolytica, Rhodotorula mucilaginosa and Penicillium brevicompactum, was first evaluated in milk agar. None of the tested pediococci showed antifungal activity while 3, 23 and 43 lactobacilli isolates showed strong antifungal activity or total inhibition against Y...
September 30, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28930236/antibacterial-and-antifungal-activity-of-essential-oils-against-pathogens-responsible-for-otitis-externa-in-dogs-and-cats
#3
Valentina V Ebani, Simona Nardoni, Fabrizio Bertelloni, Basma Najar, Luisa Pistelli, Francesca Mancianti
Background: Essential oils (EOs) are recommended by some veterinarians to treat otitis externa in pets, but data about their efficacy in scientific literature are very scant. Methods: Nine commercial EOs, from roman chamomile (Anthemis nobilis L.), star anise (Illicium verum), lavender (Lavandula hybrida), litsea (Litsea cubeba (Lour.) Pers.), basil (Ocimum basilicum L.), oregano (Origanum vulgare L. subsp. hirticum), rosemary (Rosmarinus officinalis L.), clary sage (Salvia sclarea L.), and thyme (Thymus vulgaris L...
April 21, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28922488/isolation-of-rhodotorula-mucilaginosa-from-blood-cultures-in-a-tertiary-care-hospital
#4
Iker Falces-Romero, Emilio Cendejas-Bueno, María Pilar Romero-Gómez, Julio García-Rodríguez
Rhodotorula species have traditionally been considered as one of common non-virulent environmental inhabitant. They have emerged as an opportunistic pathogen, particularly in immunocompromised hosts and most infections have been associated with intravenous catheters in these patients. We review the isolates in blood cultures of Rhodotorula mucilaginosa in our Hospital. We describe the demographic and clinical features of the cases and the antifungal susceptibility profiles of the isolates. Selected patients had an isolation of R...
September 18, 2017: Mycoses
https://www.readbyqxmd.com/read/28894295/reversible-naftifine-induced-carotenoid-depigmentation-in-rhodotorula-mucilaginosa-a-j%C3%A3-rg-f-c-harrison-causing-onychomycosis
#5
Augustin C Moț, Marcel Pârvu, Alina E Pârvu, Oana Roşca-Casian, Nicoleta E Dina, Nicolae Leopold, Radu Silaghi-Dumitrescu, Cristina Mircea
Rhodotorula mucilaginosa was isolated from a patient with onychomycosis, and identification was confirmed by morphological and cultural characteristics as well as by DNA molecular analysis. Antifungal agents naftifine (10 mg/mL, active substance in Exoderil) and bifonazole (10 mg/mL, active substance in Canespor) were tested in different concentrations to assess in vitro effects on fungal growth and carotenoid synthesis. The antifungal mechanisms of action of naftifine and bifonazole against R. mucilaginosa isolates were similar and affected the biosynthetic pathway of ergosterol...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28867945/genetic-biochemical-nutritional-and-antimicrobial-characteristics-of-pomegranate-punica-granatum-l-%C3%A2-grown-in-istria
#6
Ana Miklavčič Višnjevec, Ajda Ota, Mihaela Skrt, Bojan Butinar, Sonja Smole Možina, Nina Gunde Cimerman, Marijan Nečemer, Alenka Baruca Arbeiter, Matjaž Hladnik, Marin Krapac, Dean Ban, Milena Bučar-Miklavčič, Nataša Poklar Ulrih, Dunja Bandelj
This study characterises the genetic variability of local pomegranate (Punica granatum L.) germplasm from the Slovenian and Croatian areas of Istria. The bioactive components and antioxidant and antimicrobial properties of ethanol and water extracts of different parts of pomegranate fruit were also determined, along with their preliminary nutritional characterisation. Twenty-six different genotypes identified with microsatellite analysis indicate the great diversity of pomegranate in Istria. The pomegranate fruit ethanol extracts represent rich sources of phenolic compounds (mean value of the mass fraction in exocarp and mesocarp expressed as gallic acid is 23 and 16 mg/g, respectively)...
June 2017: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/28864393/biosorbents-based-on-esterified-starch-carrying-immobilized-oil-degrading-microorganisms
#7
D V Tarabukin, M A Torlopov, T N Shchemelinina, E M Anchugova, N N Shergina, E I Istomina, V A Belyy
The complex sorbents based on hydrophobized starch, which contain oil-degrading microorganisms, have been proposed for effective sorption and utilization of petroleum-related pollutants. The sorbents were made on the base of benzoic, lauric and stearic acid esters of starch with degrees of substitution of 0.4-1.1. The esterification of starch was carried out by the reaction with acyl chlorides of the corresponding acids in an aqueous-organic medium. The structure of the esters was studied by SEM, IR and NMR spectroscopy...
August 31, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28833163/control-of-native-spoilage-yeast-on-dealcoholized-red-wine-by-preservatives-alone-and-in-binary-mixtures
#8
Marta Sánchez-Rubio, Kamal Guerrouj, Amaury Taboada-Rodríguez, Antonio López-Gómez, Fulgencio Marín-Iniesta
In order to preserve a commercial dealcoholized red wine (DRW), a study with 4 preservatives and binary mixtures of them were performed against 2 native spoilage yeasts: Rhodotorula mucilaginosa and Saccharomyces cerevisiae. Minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) for potassium sorbate, sodium benzoate, sodium metabisulfite and dimethyl dicarbonate (DMDC) were evaluated in DRW stored at 25 °C. MICs of potassium sorbate and sodium metabisulfite were 250 and 60 mg/kg, respectively for both target strains...
August 23, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28799927/heavy-metal-tolerance-and-removal-potential-in-mixed-species-biofilm
#9
Sandra Grujić, Sava Vasić, Ljiljana Čomić, Aleksandar Ostojić, Ivana Radojević
The aim of the study was to examine heavy metal tolerance (Cd(2+), Zn(2+), Ni(2+) and Cu(2+)) of single- and mixed-species biofilms (Rhodotorula mucilaginosa and Escherichia coli) and to determine metal removal efficiency (Cd(2+), Zn(2+), Ni(2+), Cu(2+), Pb(2+) and Hg(2+)). Metal tolerance was quantified by crystal violet assay and results were confirmed by fluorescence microscopy. Metal removal efficiency was determined by batch biosorption assay. The tolerance of the mixed-species biofilm was higher than the single-species biofilms...
August 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28799153/-fungi-in-the-gut-the-gut-mycobiome
#10
Herbert Hof
Many different fungi, including yeasts and molds, can be found in the intestinal tract of humans constituting the gut mycobiome. In case the bacterial flora is altered, the fungal flora may react inversely. By a so-called fungal diet, however, the composition of the mycobiome can hardly be influenced. Whereas some fungi are only transiently present in the gut after oral uptake, others, such as Candida, Saccharomyces, Rhodotorula, Trichosporon, Geotrichum, amongst others, are members of the residential, autochthonous gut flora...
August 2017: Zeitschrift Für Gastroenterologie
https://www.readbyqxmd.com/read/28771577/a-survey-of-the-mycobiota-associated-with-larvae-of-the-black-soldier-fly-hermetia-illucens-reared-for-feed-production
#11
Ilaria Varotto Boccazzi, Matteo Ottoboni, Elena Martin, Francesco Comandatore, Lisa Vallone, Thomas Spranghers, Mia Eeckhout, Valeria Mereghetti, Luciano Pinotti, Sara Epis
Feed security, feed quality and issues surrounding the safety of raw materials are always of interest to all livestock farmers, feed manufacturers and competent authorities. These concerns are even more important when alternative feed ingredients, new product developments and innovative feeding trends, like insect-meals, are considered. The black soldier fly (Hermetia illucens) is considered a good candidate to be used as feed ingredient for aquaculture and other farm animals, mainly as an alternative protein source...
2017: PloS One
https://www.readbyqxmd.com/read/28763240/dead-biomass-of-amazon-yeast-a-new-insight-into-bioremediation-and-recovery-of-silver-by-intracellular-synthesis-of-nanoparticles
#12
Marcia R Salvadori, Rômulo A Ando, Cláudio A Oller Nascimento, Benedito Corrêa
This investigation was undertaken to describe a natural process for the removal of silver and the simultaneous recovery of Ag/Ag2O nanoparticles by dead biomass of the yeast Rhodotorula mucilaginosa. The removal of silver ions from aqueous solution and the synthesis of Ag/Ag2O nanoparticles were analyzed based on physicochemical factors and equilibrium concentration, combined with transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), and infrared spectroscopy (FTIR)...
September 19, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28741338/fungi-of-the-genus-rhodotorula-isolated-from-the-oral-cavity-of-oncologic-patients-with-colorectal-cancer
#13
Piotr Troska, Ewa Sucharzewska, Maria Dynowska, Elżbieta Ejdys
This paper concerns the frequency of occurrence and enzymatic activity of fungi of the genus Rhodotorula isolated from the oral cavity of patients with colorectal cancer. Swabs from the oral cavity were subjected to standard mycological diagnostic procedures; enzymatic activity of the fungi was evaluated using and API Zym test by BioMereux. The fungi of the genus Rhodotorula were noted in 22.5% positive isolates originating from women and 25.7% positive isolates originating from men. They included mono-species isolates of: Rh...
2017: Annals of Parasitology
https://www.readbyqxmd.com/read/28736890/insect-frass-in-stored-cereal-products-as-a-potential-source-of-lactobacillus-sanfranciscensis-for-sourdough-ecosystem
#14
F Boiocchi, D Porcellato, L Limonta, C Picozzi, I Vigentini, D P Locatelli, R Foschino
AIM: Insect frass samples were collected from Drosophila melanogaster, Plodia interpunctella, Rhyzopertha dominica, Sitophilus granarius, Sitophilus oryzae, Sitophilus zeamais, Tribolium confusum and Tribolium castaneum to elucidate if they can be the origin of Type I sourdough micro-organisms (Lactobacillus sanfranciscensis and Candida milleri). METHODS AND RESULTS: Selective enrichments were carried out to isolate lactic acid bacteria (LBA) and yeast. A metagenetic analysis, targeted on bacterial 16S rRNA gene and fungal ITS region, was performed by using Illumina MiSeq protocol...
July 23, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28736146/lipid-accumulation-potential-of-oleaginous-yeasts-a-comparative-evaluation-using-food-waste-leachate-as-a-substrate
#15
Davidraj Johnravindar, Obulisamy Parthiba Karthikeyan, Ammaiyappan Selvam, Kumarasamy Murugesan, Jonathan W C Wong
In present study, the efficiency of three oleaginous yeasts i.e., Yarrowia lipolytica, Rhodotorula glutinis and Cryptococcus curvatus were compared for their lipid assimilation capacities using three different FW-leachates as a medium. The FW-leachates were collected from dry anaerobic digesters and diluted to achieve carbohydrate content of 25gL(-1) prior to yeast inoculations. Around 5% of yeast cultures were individually mixed in three different FW-leachate mediums and incubated under 30°C and 150rpm agitation for 6days...
June 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28730647/swimming-pools-and-fungi-an-epidemiology-survey-in-polish-indoor-swimming-facilities
#16
Marek Jankowski, Anna Charemska, Rafał Czajkowski
Environmental conditions for swimming facilities may support fungal growth and also may represent a biological risk for users. However, since previously published studies both cleaning procedures and sanitary regulations are likely to have been improved. The aim of the study was to examine whether attendance to public swimming pools was still a risk factor for fungal infection of the skin in Poland. We investigated the occurrence of mycotic species, in a sample from Polish swimming pools. Detection and identification of isolated species were achieved by cultural and morphological methods...
July 20, 2017: Mycoses
https://www.readbyqxmd.com/read/28729072/recycling-of-food-waste-for-fuel-precursors-using-an-integrated-bio-refinery-approach
#17
O P Karthikeyan, H T Nguyen Hao, Ali Razaghi, Kirsten Heimann
The main aim was to integrate FW-recycling with cultivation of Rhodotorula glutinis and anaerobic digestion (AD) for bio-energy and -fuel recovery. Mixed FW was mechanically macerated (Pcon) and hydrolysed (at 250gL(-1) water) via chemical (Ch), thermal (Th) and TCh (combined Ch and Th) treatments. Cleared hydrolysates from individual pre-treatment processes were used as culture medium for cultivation of R. glutinis, while the residual solids (RS) were subjected to AD. Pcon cultivation yielded maximal R. glutinis dry weight biomass (5...
June 26, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28713362/biocontrol-agents-increase-the-specific-rate-of-patulin-production-by-penicillium-expansum-but-decrease-the-disease-and-total-patulin-contamination-of-apples
#18
Xiangfeng Zheng, Qiya Yang, Xiaoyun Zhang, Maurice T Apaliya, Giuseppe Ianiri, Hongyin Zhang, Raffaello Castoria
Synthetic fungicides are commonly employed for the control of postharvest diseases of fruits. However, due to health concerns about the use of these chemicals, alternative control methods including biocontrol based on antagonistic yeasts are gaining in popularity. In this study, we investigated the effects of two biocontrol yeasts, Rhodotorula mucilaginosa strain 3617 and Rhodotorula kratochvilovae strain LS11, on blue mold and patulin (PAT) contamination caused by Penicillium expansum strains PY and FS7 in artificially inoculated Fuji apples stored at 20°C for 9 days...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28701186/the-oral-fungal-mycobiome-characteristics-and-relation-to-periodontitis-in-a-pilot-study
#19
Brandilyn A Peters, Jing Wu, Richard B Hayes, Jiyoung Ahn
BACKGROUND: The oral fungal microbiome (mycobiome) is not well characterized, particularly in relation to oral diseases such as periodontal disease. We aimed to describe and compare the oral mycobiome of subjects with and without periodontal disease. RESULTS: We characterized the oral mycobiome in 30 adult subjects (15 with periodontal disease, 15 with good oral health) by sequencing the taxonomically informative pan-fungal internal transcribed spacer (ITS) gene in DNA extracted from oral wash samples...
July 12, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28695189/risk-of-fungal-infection-to-dental-patients
#20
Jaqueline Lopes Damasceno, Rafael Aparecido Dos Santos, Amir Horiquini Barbosa, Luciana Assirati Casemiro, Regina Helena Pires, Carlos Henrique Gomes Martins
Fungi can cause various diseases, and some pathogenic fungi have been detected in the water of dental equipment. This environment offers suitable conditions for fungal biofilms to emerge, which can facilitate mycological contamination. This study verified whether the water employed in the dental units of two dental clinics at the University of Franca was contaminated with fungi. This study also evaluated the ability of the detected fungi to form biofilms. The high-revving engine contained the largest average amount of fungi, 14...
2017: TheScientificWorldJournal
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