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https://www.readbyqxmd.com/read/28799927/heavy-metal-tolerance-and-removal-potential-in-mixed-species-biofilm
#1
Sandra Grujić, Sava Vasić, Ljiljana Čomić, Aleksandar Ostojić, Ivana Radojević
The aim of the study was to examine heavy metal tolerance (Cd(2+), Zn(2+), Ni(2+) and Cu(2+)) of single- and mixed-species biofilms (Rhodotorula mucilaginosa and Escherichia coli) and to determine metal removal efficiency (Cd(2+), Zn(2+), Ni(2+), Cu(2+), Pb(2+) and Hg(2+)). Metal tolerance was quantified by crystal violet assay and results were confirmed by fluorescence microscopy. Metal removal efficiency was determined by batch biosorption assay. The tolerance of the mixed-species biofilm was higher than the single-species biofilms...
August 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28799153/-fungi-in-the-gut-the-gut-mycobiome
#2
Herbert Hof
Many different fungi, including yeasts and molds, can be found in the intestinal tract of humans constituting the gut mycobiome. In case the bacterial flora is altered, the fungal flora may react inversely. By a so-called fungal diet, however, the composition of the mycobiome can hardly be influenced. Whereas some fungi are only transiently present in the gut after oral uptake, others, such as Candida, Saccharomyces, Rhodotorula, Trichosporon, Geotrichum, amongst others, are members of the residential, autochthonous gut flora...
August 2017: Zeitschrift Für Gastroenterologie
https://www.readbyqxmd.com/read/28771577/a-survey-of-the-mycobiota-associated-with-larvae-of-the-black-soldier-fly-hermetia-illucens-reared-for-feed-production
#3
Ilaria Varotto Boccazzi, Matteo Ottoboni, Elena Martin, Francesco Comandatore, Lisa Vallone, Thomas Spranghers, Mia Eeckhout, Valeria Mereghetti, Luciano Pinotti, Sara Epis
Feed security, feed quality and issues surrounding the safety of raw materials are always of interest to all livestock farmers, feed manufacturers and competent authorities. These concerns are even more important when alternative feed ingredients, new product developments and innovative feeding trends, like insect-meals, are considered. The black soldier fly (Hermetia illucens) is considered a good candidate to be used as feed ingredient for aquaculture and other farm animals, mainly as an alternative protein source...
2017: PloS One
https://www.readbyqxmd.com/read/28763240/dead-biomass-of-amazon-yeast-a-new-insight-into-bioremediation-and-recovery-of-silver-by-intracellular-synthesis-of-nanoparticles
#4
Marcia R Salvadori, Rômulo A Ando, Cláudio A Oller Nascimento, Benedito Corrêa
This investigation was undertaken to describe a natural process for the removal of silver and the simultaneous recovery of Ag/Ag2O nanoparticles by dead biomass of the yeast Rhodotorula mucilaginosa. The removal of silver ions from aqueous solution and the synthesis of Ag/Ag2O nanoparticles were analyzed based on physicochemical factors and equilibrium concentration, combined with transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), and infrared spectroscopy (FTIR)...
August 1, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28741338/fungi-of-the-genus-rhodotorula-isolated-from-the-oral-cavity-of-oncologic-patients-with-colorectal-cancer
#5
Piotr Troska, Ewa Sucharzewska, Maria Dynowska, Elżbieta Ejdys
This paper concerns the frequency of occurrence and enzymatic activity of fungi of the genus Rhodotorula isolated from the oral cavity of patients with colorectal cancer. Swabs from the oral cavity were subjected to standard mycological diagnostic procedures; enzymatic activity of the fungi was evaluated using and API Zym test by BioMereux. The fungi of the genus Rhodotorula were noted in 22.5% positive isolates originating from women and 25.7% positive isolates originating from men. They included mono-species isolates of: Rh...
2017: Annals of Parasitology
https://www.readbyqxmd.com/read/28736890/insect-frass-in-stored-cereal-products-as-a-potential-source-of-lactobacillus-sanfranciscensis-for-sourdough-ecosystem
#6
Federica Boiocchi, Davide Porcellato, Lidia Limonta, Claudia Picozzi, Ileana Vigentini, Daria Patrizia Locatelli, Roberto Foschino
AIM: Insect frass samples were collected from Drosophila melanogaster, Plodia interpunctella, Rhyzopertha dominica, Sitophilus granarius, S. oryzae, S. zeamais, Tribolium confusum and T. castaneum to elucidate if they can be the origin of Type I sourdough microorganisms (Lactobacillus sanfranciscensis and Candida milleri). METHODS AND RESULTS: Selective enrichments were carried out to isolate Lactic Acid Bacteria and yeast. A metagenetic analysis, targeted on bacterial 16S rRNA gene and fungal ITS region, was performed by using Illumina MiSeq protocol...
July 23, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28736146/lipid-accumulation-potential-of-oleaginous-yeasts-a-comparative-evaluation-using-food-waste-leachate-as-a-substrate
#7
Davidraj Johnravindar, Obulisamy Parthiba Karthikeyan, Ammaiyappan Selvam, Kumarasamy Murugesan, Jonathan W C Wong
In present study, the efficiency of three oleaginous yeasts i.e., Yarrowia lipolytica, Rhodotorula glutinis and Cryptococcus curvatus were compared for their lipid assimilation capacities using three different FW-leachates as a medium. The FW-leachates were collected from dry anaerobic digesters and diluted to achieve carbohydrate content of 25gL(-1) prior to yeast inoculations. Around 5% of yeast cultures were individually mixed in three different FW-leachate mediums and incubated under 30°C and 150rpm agitation for 6days...
June 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28730647/swimming-pools-and-fungi-an-epidemiology-survey-in-polish-indoor-swimming-facilities
#8
Marek Jankowski, Anna Charemska, Rafał Czajkowski
Environmental conditions for swimming facilities may support fungal growth and also may represent a biological risk for users. However, since previously published studies both cleaning procedures and sanitary regulations are likely to have been improved. The aim of the study was to examine whether attendance to public swimming pools was still a risk factor for fungal infection of the skin in Poland. We investigated the occurrence of mycotic species, in a sample from Polish swimming pools. Detection and identification of isolated species were achieved by cultural and morphological methods...
July 20, 2017: Mycoses
https://www.readbyqxmd.com/read/28729072/recycling-of-food-waste-for-fuel-precursors-using-an-integrated-bio-refinery-approach
#9
O P Karthikeyan, H T Nguyen Hao, Ali Razaghi, Kirsten Heimann
The main aim was to integrate FW-recycling with cultivation of Rhodotorula glutinis and anaerobic digestion (AD) for bio-energy and -fuel recovery. Mixed FW was mechanically macerated (Pcon) and hydrolysed (at 250gL(-1) water) via chemical (Ch), thermal (Th) and TCh (combined Ch and Th) treatments. Cleared hydrolysates from individual pre-treatment processes were used as culture medium for cultivation of R. glutinis, while the residual solids (RS) were subjected to AD. Pcon cultivation yielded maximal R. glutinis dry weight biomass (5...
June 26, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28713362/biocontrol-agents-increase-the-specific-rate-of-patulin-production-by-penicillium-expansum-but-decrease-the-disease-and-total-patulin-contamination-of-apples
#10
Xiangfeng Zheng, Qiya Yang, Xiaoyun Zhang, Maurice T Apaliya, Giuseppe Ianiri, Hongyin Zhang, Raffaello Castoria
Synthetic fungicides are commonly employed for the control of postharvest diseases of fruits. However, due to health concerns about the use of these chemicals, alternative control methods including biocontrol based on antagonistic yeasts are gaining in popularity. In this study, we investigated the effects of two biocontrol yeasts, Rhodotorula mucilaginosa strain 3617 and Rhodotorula kratochvilovae strain LS11, on blue mold and patulin (PAT) contamination caused by Penicillium expansum strains PY and FS7 in artificially inoculated Fuji apples stored at 20°C for 9 days...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28701186/the-oral-fungal-mycobiome-characteristics-and-relation-to-periodontitis-in-a-pilot-study
#11
Brandilyn A Peters, Jing Wu, Richard B Hayes, Jiyoung Ahn
BACKGROUND: The oral fungal microbiome (mycobiome) is not well characterized, particularly in relation to oral diseases such as periodontal disease. We aimed to describe and compare the oral mycobiome of subjects with and without periodontal disease. RESULTS: We characterized the oral mycobiome in 30 adult subjects (15 with periodontal disease, 15 with good oral health) by sequencing the taxonomically informative pan-fungal internal transcribed spacer (ITS) gene in DNA extracted from oral wash samples...
July 12, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28695189/risk-of-fungal-infection-to-dental-patients
#12
Jaqueline Lopes Damasceno, Rafael Aparecido Dos Santos, Amir Horiquini Barbosa, Luciana Assirati Casemiro, Regina Helena Pires, Carlos Henrique Gomes Martins
Fungi can cause various diseases, and some pathogenic fungi have been detected in the water of dental equipment. This environment offers suitable conditions for fungal biofilms to emerge, which can facilitate mycological contamination. This study verified whether the water employed in the dental units of two dental clinics at the University of Franca was contaminated with fungi. This study also evaluated the ability of the detected fungi to form biofilms. The high-revving engine contained the largest average amount of fungi, 14...
2017: TheScientificWorldJournal
https://www.readbyqxmd.com/read/28681129/simultaneous-production-of-intracellular-triacylglycerols-and-extracellular-polyol-esters-of-fatty-acids-by-rhodotorula-babjevae-and-rhodotorula-aff-paludigena
#13
Luis A Garay, Irnayuli R Sitepu, Tomas Cajka, Erin Cathcart, Oliver Fiehn, J Bruce German, David E Block, Kyria L Boundy-Mills
Microbial oils have been analyzed as alternatives to petroleum. However, just a handful of microbes have been successfully adapted to produce chemicals that can compete with their petroleum counterparts. One of the reasons behind the low success rate is the overall economic inefficiency of valorizing a single product. This study presents a lab-scale analysis of two yeast species that simultaneously produce multiple high-value bioproducts: intracellular triacylglycerols (TG) and extracellular polyol esters of fatty acids (PEFA), two lipid classes with immediate applications in the biofuels and surfactant industries...
July 5, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28669678/virulence-and-antifungal-therapy-of-murine-disseminated-infection-by-rhodotorula-mucilaginosa
#14
Pamela Thomson, Loida López-Fernández, Josep Guarro, Javier Capilla
Rhodotorula infections have emerged in recent years causing mainly fungemia associated to high mortality. We have evaluated the in vitro activity of nine antifungal drugs against four clinical strains of Rhodotorula mucilaginosa, being amphotericin B, voriconazole and posaconazole the most active compounds. The experimental virulence of this fungus and the efficacy of the three mentioned drugs were evaluated in disseminated infections in neutropenic mice. Infection resulted in a high fungal load in all the organs studied without evident particular tropism...
June 16, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28662427/oral-mycoses-in-avian-scavengers-exposed-to-antibiotics-from-livestock-farming
#15
Aida Pitarch, Concha Gil, Guillermo Blanco
The exposure to antimicrobial pharmaceuticals as environmental contaminants can exert direct and indirect detrimental effects on health of wildlife. Fungal infections pose a major threat to domestic, captive-housed wild and free-ranging wild animals worldwide. However, little is known about their role in disease in birds in the wild. Here, we evaluated the incidence of thrush-like lesions in the oral cavity of wild nestling cinereous vultures (Aegypius monachus), griffon vultures (Gyps fulvus), Egyptian vultures (Neophron percnopterus) and golden eagles (Aquila chrysaetos) exposed to veterinary antibiotics via the consumption of medicated livestock carcasses...
December 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28660362/antarctic-rocks-from-continental-antarctica-as-source-of-potential-human-opportunistic-fungi
#16
Vívian N Gonçalves, Fabio S Oliveira, Camila R Carvalho, Carlos E G R Schaefer, Carlos A Rosa, Luiz H Rosa
We assessed the diversity of culturable fungi associated with rocks of continental Antarctica to evaluate their physiological opportunistic virulence potential in vitro. The seventy fungal isolates obtained were identified as nine species of Acremonium, Byssochlamys, Cladosporium, Debaryomyces, Penicillium, and Rhodotorula. Acremonium sp., D. hansenii, P. chrysogenum, P. citrinum, P. tardochrysogenum, and R. mucilaginosa were able to grow at 37 °C; in addition, B. spectabilis displayed a high level of growth at 37 and 45 °C...
June 28, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/28648496/fruits-as-the-vehicle-of-drug-resistant-pathogenic-yeasts
#17
Hsiu-Jung Lo, Sheng-Hua Tsai, Wen-Li Chu, Yin-Zhi Chen, Zi-Li Zhou, Hong-Fu Chen, Ching-Fu Lee, Yun-Liang Yang
OBJECTIVE: We investigated the diversity and drug susceptibility of pathogenic yeasts on fruit surfaces. METHOD: Fruits were purchased from supermarkets and washed with buffer. The pellets were re-suspended in medium after centrifugation. The cell suspensions were plated onto CHROMagar Candida medium. Yeasts were identified by ribosomal DNA sequencing and their drug susceptibilities were determined by broth microdilution assay. RESULTS: Of 184 isolates, comprised of 55 species, from 22 different types of fruits, 29 species, including Candida famata, Candida fermentati, Candida guilliermondii, Candida intermedia, Candida krusei, Candida orthopsilosis, Candida parapsilosis, Candida pelliculosa, Candida tropicalis, and others have been reported to cause diseases in humans...
June 23, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28639705/cloning-and-characterization-of-iron-superoxide-dismutase-in-antarctic-yeast-strain-rhodotorula-mucilaginosa-an5
#18
Guangfeng Kan, Hua Wen, Xiaofei Wang, Ting Zhou, Cuijuan Shi
A novel superoxide dismutase gene from Antarctic yeast Rhodotorula mucilaginosa AN5 was cloned, sequenced, and then expressed in Escherichia coli. The R. mucilaginosa AN5 SOD (RmFeSOD) gene was 639 bp open reading frame in length, which encoded a protein of 212 amino acids with a deduced molecular mass of 23.5 kDa and a pI of 7.89. RmFeSOD was identified as iron SOD type with a natural status of homodimer. The recombinant RmFeSOD showed good pH stability in the pH 1.0-9.0 after 1 h incubation. Meanwhile, it was found to behave relatively high thermostability, and maintained more than 80% activity at 50 °C for 1 h...
June 22, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28615057/erratum-to-engineering-an-efficient-and-tight-d-amino-acid-inducible-gene-expression-system-in-rhodosporidium-rhodotorula-species
#19
Yanbin Liu, Chong Mei John Koh, Si Te Ngoh, Lianghui Ji
No abstract text is available yet for this article.
June 14, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28597157/optimization-of-cultivation-conditions-for-biotechnological-production-of-lipid-by-rhodotorula-kratochvilovae-syn-rhodosporidium-kratochvilovae-sy89-for-biodiesel-preparation
#20
Tamene Milkessa Jiru, Marizeth Groenewald, Carolina Pohl, Laurinda Steyn, Nicholas Kiggundu, Dawit Abate
Rhodotorula kratochvilovae (syn, Rhodosporidium kratochvilovae) SY89, an oleaginous yeast, isolated from Ethiopian soil, was grown under nitrogen-limited media. The capacity this with respect to biomass production, lipid yield and lipid content was evaluated. The influence of inoculum size, carbon sources, variations in glucose concentration, nitrogen sources, C/N ratio, pH, temperature, agitation, and aeration rate and incubation period were investigated. Inoculum size of 10% v/v, glucose as a carbon source at 50 g/L glucose, 0...
June 2017: 3 Biotech
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