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https://www.readbyqxmd.com/read/29676766/yeast-isolation-and-identification-in-water-used-in-a-brazilian-hemodialysis-unit-by-classic-microbiological-techniques-and-raman-spectroscopy
#1
Lilian Bueno Montanari, Flávio Garcia Sartori, Diórginis Bueno Montrazi Ribeiro, Luís Fernando Leandro, Regina Helena Pires, Márcia de Souza Carvalho Melhem, César Alexandre de Mello, Carlos Henrique Gomes Martins
The use of poorly treated water in hemodialysis centers may lead to fungal contamination, which poses a serious threat to immunologically debilitated hemodialysis patients. This study aimed to isolate and identify yeast species in the water of a Brazilian hemodialysis center by using classic microbiological techniques and Raman spectroscopy. For 12 months, a total of 288 water samples were collected from different points of the hemodialysis treatment distribution center. One hundred and forty-six yeast species were isolated and identified in the samples that tested positive for the presence of yeasts such as Candida parapsilosis (100 isolates, or 68...
April 2018: Journal of Water and Health
https://www.readbyqxmd.com/read/29661607/catheter-related-bloodstream-infection-due-to-rhodotorula-mucilaginosa-with-normal-serum-1%C3%A2-3-%C3%AE-d-glucan-level
#2
T Kitazawa, S Ishigaki, K Seo, Y Yoshino, Y Ota
Rhodotorula species are environmental basidiomycete yeasts that have emerged as a cause of fungemia in immunocompromised hosts. The insertion of a central venous catheter was identified as a major risk factor for Rhodotorula fungemia. Few cases reports have reported (1→3)-β-D-glucan testing at the onset of Rhodotorula mucilaginosa fungemia. We report a case of catheter-related bloodstream infection due to R. mucilaginosa. Serum β-D-glucan level was normal at the onset of the bloodstream infection. It took 5 days to culture the isolate...
April 13, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29602635/yeasts-and-filamentous-fungi-associated-with-some-dairy-products-in-egypt
#3
A-A H Moubasher, M A Abdel-Sater, Z S M Soliman
This work aimed to identify and evaluate the diversity of yeasts and filamentous fungi that contaminate some dairy products (fresh buffalo milk, plain yoghurt, butter and Kareisk cheese) in Assiut city, Egypt. The identification was based on phenotypic characteristics, and genotypically in case of yeasts. The pHs of all dairy products lie within the acidic range, but yoghurt registered the highest value. A total of 41 genera and 89 species+3 varieties were recovered from all dairy products investigated on DRBC (26 genera representing 59 species+1 variety), DG18 (32 genera, 56 species+2 varieties) and MY50G (31 genera, 64 species+2 varieties)...
March 27, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29587755/torulene-and-torularhodin-new-fungal-carotenoids-for-industry
#4
REVIEW
Anna M Kot, Stanisław Błażejak, Iwona Gientka, Marek Kieliszek, Joanna Bryś
Torulene and torularhodin represent the group of carotenoids and are synthesized by yeasts and fungi. The most important producers of these two compounds include yeasts of Rhodotorula and Sporobolomyces genera. The first reports confirming the presence of torulene and torularhodin in the cells of microorganisms date to the 1930s and 1940s; however, only in the past few years, the number of works describing the properties of these compounds increased. These compounds have strong anti-oxidative and anti-microbial properties, and thus may be successfully used as food, feedstock, and cosmetics additives...
March 27, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29551441/-a-skin-ulcer-infection-due-to-rhodotorula-mucilaginosa-in-an-immunocompromised-child
#5
H Tligui, W Oudaina, S El Ftouh, F Madda, L Hesseissen
Human cutaneous infections due to Rhodotorula species as an emerged pathogens yeasts are rarely reported, particularly in skin folds, with the ability to infect immunocompromised as well as immunocompetent patient. In present case, we describe a fungal cutaneous infection due to Rhodotorula mucilaginosa in immunocompromised child, the patient has been diagnosed with retinoblastoma; the diagnosis was established by the cytological and clinical examination. However, the cytological examination found fungal forms...
March 15, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29521624/functional-genomics-of-lipid-metabolism-in-the-oleaginous-yeast-rhodosporidium-toruloides
#6
Samuel T Coradetti, Dominic Pinel, Gina Geiselman, Masakazu Ito, Stephen Mondo, Morgann C Reilly, Ya-Fang Cheng, Stefan Bauer, Igor Grigoriev, John M Gladden, Blake A Simmons, Rachel Brem, Adam P Arkin, Jeffrey M Skerker
The basidiomycete yeast Rhodosporidium toruloides (a.k.a. Rhodotorula toruloides ) accumulates high concentrations of lipids and carotenoids from diverse carbon sources. It has great potential as a model for the cellular biology of lipid droplets and for sustainable chemical production. We developed a method for high-throughput genetics (RB-TDNAseq), using sequence-barcoded Agrobacterium tumefaciens T-DNA insertions. We identified 1337 putative essential genes with low T-DNA insertion rates. We functionally profiled genes required for fatty acid catabolism and lipid accumulation, validating results with 35 targeted deletion strains...
March 9, 2018: ELife
https://www.readbyqxmd.com/read/29520720/microbial-community-and-physicochemical-dynamics-during-the-production-of-chicha-a-traditional-beverage-of-indigenous-people-of-brazil
#7
Ludmila Vilela Resende, Leticia Kleinhans Pinheiro, Maria Gabriela da Cruz Pedroso Miguel, Cíntia Lacerda Ramos, Danielle Marques Vilela, Rosane Freitas Schwan
The microbial community of artisanal corn fermentation called Chicha were isolated, purified and then identified using protein profile by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) and confirmed by partial ribosomal gene sequencing. Samples from Chicha beverage were chemically characterized by gas and liquid chromatography (HPLC and GC-MS). Aerobic mesophilic bacteria (AMB) (35.8% of total of isolated microorganisms), lactic acid bacteria (LAB) (21.6%) and yeast (42...
March 8, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29519831/complete-genome-sequence-of-the-biocontrol-agent-yeast-rhodotorula-kratochvilovae-strain-ls11
#8
Cecilia Miccoli, Davide Palmieri, Filippo De Curtis, Giuseppe Lima, Giuseppe Ianiri, Raffaello Castoria
Rhodotorula kratochvilovae strain LS11 is a biocontrol agent (BCA) selected for its antagonistic activity against several plant pathogens both in the field and postharvest. Genome assembly includes 62 contigs for a total of 22.56 Mbp and a G+C content of 66.6%. Genome annotation predicts 7,642 protein-encoding genes.
March 8, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29509674/amperometric-self-referencing-ceramic-based-microelectrode-arrays-for-d-serine-detection
#9
Diana Campos-Beltrán, Åsa Konradsson-Geuken, Jorge E Quintero, Lisa Marshall
D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophrenia. Thus, it is of increasing interest to monitor the concentration of D-serine in vivo as a relevant player in dynamic neuron-glia network activity. Here we present a procedure for amperometric detection of D-serine with self-referencing ceramic-based microelectrode arrays (MEAs) coated with D-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO)...
March 6, 2018: Biosensors
https://www.readbyqxmd.com/read/29492690/both-plant-genotype-and-herbivory-shape-aspen-endophyte-communities
#10
Benedicte Riber Albrectsen, Abu Bakar Siddique, Vicki Huizu Guo Decker, Martin Unterseher, Kathryn M Robinson
Salicinoid phenolic glycosides are common defence substances in salicaceous trees and specialist leaf beetles use these compounds for their own defence against predators. Salicinoids vary qualitatively and qualitatively in aspen (Populus tremula) and this variation has a genetic basis. The foliar endophyte mycobiome is plentiful and we hypothesised that it is related to plant genotype, potentially mediated by salicinoid composition, and that interactions with the leaf beetle Chrysomela tremula may alter this relationship...
March 1, 2018: Oecologia
https://www.readbyqxmd.com/read/29466942/identification-of-novel-genes-in-the-carotenogenic-and-oleaginous-yeast-rhodotorula-toruloides-through-genome-wide-insertional-mutagenesis
#11
Yanbin Liu, Chong Mei John Koh, Sihui Amy Yap, Minge Du, Mya Myintzu Hlaing, Lianghui Ji
BACKGROUND: Rhodotorula toruloides is an outstanding producer of lipids and carotenoids. Currently, information on the key metabolic pathways and their molecular basis of regulation remains scarce, severely limiting efforts to engineer it as an industrial host. RESULTS: We have adapted Agrobacterium tumefaciens-mediated transformation (ATMT) as a gene-tagging tool for the identification of novel genes in R. toruloides. Multiple factors affecting transformation efficiency in several species in the Pucciniomycotina subphylum were optimized...
February 21, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29464844/dietary-live-yeast-and-increased-water-temperature-influence-the-gut-microbiota-of-rainbow-trout
#12
David Huyben, Li Sun, Richard Moccia, Anders Kiessling, Johan Dicksved, Torbjörn Lundh
AIMS: The objective was to determine the effects of dietary substitution of fishmeal with live yeast and increasing water temperature on the diversity and composition of gut microbiota in rainbow trout. METHODS AND RESULTS: Fish were fed either fishmeal or yeast (Saccharomyces cerevisiae) and reared in water temperatures of either 11 °C (cold) or 18 °C (warm) for six weeks. Luminal content and mucosa were collected from the distal gut and the load, diversity and species abundance of yeast and bacteria were analysed using agar plating, MALDI-TOF and rRNA gene amplicon sequencing...
February 21, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29453061/effect-of-u-vi-aqueous-speciation-on-the-binding-of-uranium-by-the-cell-surface-of-rhodotorula-mucilaginosa-a-natural-yeast-isolate-from-bentonites
#13
Margarita Lopez-Fernandez, Maria Romero-González, Alix Günther, Pier L Solari, Mohamed L Merroun
This study presents the effect of aqueous uranium speciation (U-hydroxides and U-hydroxo-carbonates) on the interaction of this radionuclide with the cells of the yeast Rhodotorula mucigilanosa BII-R8. This strain was isolated from Spanish bentonites considered as reference materials for the engineered barrier components of the future deep geological repository of radioactive waste. X-ray absorption and infrared spectroscopy showed that the aqueous uranium speciation has no effect on the uranium binding process by this yeast strain...
February 9, 2018: Chemosphere
https://www.readbyqxmd.com/read/29442377/a-cold-and-organic-solvent-tolerant-lipase-produced-by-antarctic-strain-rhodotorula-sp-y-23
#14
Abhas K Maharana, Shiv M Singh
Psychrotolerant yeast Rhodotorula sp. Y-23 was isolated from the sediment core sub-samples of Nella Lake, East Antarctica. Isolate was screened for lipase production using plate assay method followed by submerged fermentation. Production optimization revealed the maximum lipase production by using palmolein oil (5% v/v), pH 8.0 and inoculum size of 2.5% v/v at 15 °C. The potential inducers for lipase were 1% w/v of galactose and KNO3, and MnCl2(0.1% w/v). Final productions with optimized conditions gave 5...
February 14, 2018: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29433238/disinfection-efficacy-over-yeast-biofilms-of-juice-processing-industries
#15
María C Tarifa, Jorge E Lozano, Lorena I Brugnoni
Membrane separation systems represent a hot - spot for biofilm formation in juice industries. Sodium hypochlorite (NaOCl) has been traditionally the disinfectant of choice; however, its effectiveness over well-established biofilms is limited. In this work the study of biofilm formation on ultrafiltration membranes was proposed. The effectiveness of cleaning and disinfection procedures commonly used in juice industry was tested on the removal and killing of cells. The species used (Rhodotorula mucilaginosa, Candida krusei, Candida kefyr and Candida tropicalis) were isolated from ultrafiltration modules of a clarified apple juice industry...
March 2018: Food Research International
https://www.readbyqxmd.com/read/29428422/yeasts-from-skin-colonization-are-able-to-cross-the-acellular-dermal-matrix
#16
Isabele Carrilho Jarros, Érika Okuno, Maiara Ignacio Costa, Flávia Franco Veiga, Patricia de Souza Bonfim-Mendonça, Melyssa Fernanda Norman Negri, Terezinha Inez Estivalet Svidzinski
In recent decades, the prognosis for burn patients has improved considerably with the development of specialized care. The acellular dermal matrix (ADM) is a totally artificial acellular device that functions to control water loss, prevent penetration by bacteria and allow migration of endothelial cells and fibroblasts from patient tissues. However, little is known about its effectiveness against yeasts. The present study evaluated the capacity of colonization and migration of some human commensal yeasts. Three clinical isolates from skin scales, identified as Candida parapsilosis, Candida glabrata and Rhodotorula mucilaginosa, were used...
February 8, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29424627/bioprospecting-microbes-for-single-cell-oil-production-from-starchy-wastes
#17
Shivani Chaturvedi, Arti Kumari, Lata Nain, Sunil K Khare
Production of lipid from oleaginous yeast using starch as carbon source is not a common practice, therefore the purpose of this investigation was to explore the capability of starch assimilating microbes to produce oil, which was determined in terms of biomass weight, productivity & lipid yield. Saccharomyces pastorianus, Rhodotorula mucilaginosa, R. glutinis, and fungal isolate Ganoderma wiiroense were screened for the key parameters. The optimization was also carried out by one factor at a time approach. Considering the specific yield of lipid and cell dry weight yield, R...
February 9, 2018: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/29421668/first-report-of-sporadic-cases-of-candida-auris-in-colombia
#18
Claudia M Parra-Giraldo, Sandra L Valderrama, Gloria Cortes-Fraile, Javier R Garzón, Beatriz E Ariza, Florent Morio, Melva Y Linares-Linares, Andrés Ceballos-Garzón, Alejandro de la Hoz, Catalina Hernandez, Carlos Alvarez-Moreno, Patrice Le Pape
BACKGROUND: Candida auris is a recently reported Candida species which is phenotypically similar to Candida haemulonii and related to hospital outbreaks. This organism can be misidentified as C. haemulonii, C. famata, C. catenulata or Rhodotorula glutinis by phenotypic approaches. MALDI-TOF MS and DNA sequence analysis using ITS rDNA barcoding provide an accurate identification. CASE PRESENTATIONS: Three cases of C. auris infection in patients with risk factors for fungal infection (Admitted to the intensive care unit, lymphoma and HIV respectively, all three with previous antibiotic use) and not epidemiologically related...
February 5, 2018: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29414159/enhanced-single-cell-oil-production-by-mixed-culture-of-chlorella-pyrenoidosa-and-rhodotorula-glutinis-using-cassava-bagasse-hydrolysate-as-carbon-source
#19
Lu Liu, Junhui Chen, Phaik-Eem Lim, Dong Wei
The single cell oil (SCO) production by the mono and mixed culture of microalgae Chlorella pyrenoidosa and red yeast Rhodotorula glutinis was investigated using non-detoxified cassava bagasse hydrolysate (CBH) as carbon source. The results suggested that the two strains were able to tolerate and even degrade some byproducts presented in the CBH, and the mixed culture approach enhanced the degradation of certain byproducts. Biomass (20.37 ± 0.38 g/L) and lipid yield (10.42 ± 1.21 g/L) of the mixed culture achieved in the batch culture were significantly higher than that of the mono-cultures (p < 0...
February 2, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29387377/mycofloral-profile-and-the-radiation-sensitivity-d10-values-of-solar-dried-and-gamma-irradiated-pleurotus-ostreatus-jacq-ex-fr-kummer-fruitbodies-stored-in-two-different-packaging-materials
#20
Nii Korley Kortei, George Tawia Odamtten, Mary Obodai, Michael Wiafe-Kwagyan
The presence of fungi in our foods poses serious health risks as some genera of fungi may produce certain mycotoxins which have carcinogenic, mutagenic, teratogenic, and immunosuppressive effect on humans and animals alike. Fruitbodies of Pleurotus ostreatus were solar dried at a moisture content of 12.5 ± 0.2% and stored in polythene and polypropylene packs, gamma irradiated at doses of 0 (control), 1, 2, 3, 4, and 5 kGy at a dose rate of 1.7 kGy/hr from a Cobalt 60 source (SLL, 515, Hungary) and stored at room temperature 28-30°C for a period of 12 months...
January 2018: Food Science & Nutrition
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