keyword
MENU ▼
Read by QxMD icon Read
search

uridine monophosphate

keyword
https://www.readbyqxmd.com/read/29283068/influence-of-genetic-polymorphisms-on-mycophenolic-acid-pharmacokinetics-and-patient-outcomes-in-renal-transplantation
#1
Miao Guo, Zi-Jie Wang, Hai-Wei Yang, Ling Meng, Ruo-Yun Tan, Min Gu, Ji-Fu Wei
Mycophenolic acid (MPA) is an immunosuppressive drug widely used in the treatment of organ transplantation and autoimmune diseases. Pharmacokinetics and pharmacodynamics of MPA varies between individuals, the potential reasons being the genetic polymorphisms in key enzymes, drug transporters and target proteins of MPA, involving uridine diphosphate glucuronosyltransferase enzymes, organic anion transport polypeptides, multidrug resistance-associated protein 2, inosine monophosphate dehydrogenase and others...
December 27, 2017: Current Drug Metabolism
https://www.readbyqxmd.com/read/29273880/computational-analysis-of-the-effect-of-polymerase-acidic-pa-gene-mutation-f35l-in-the-2009-pandemic-influenza-a-h1n1-virus-on-binding-aspects-of-mononucleotides-in-the-endonuclease-domain
#2
Dipali Bhoye, Sarah S Cherian
An F35L mutation in the N-terminal domain of the polymerase acidic protein (PA-Nter), which contains the active site of the endonuclease, has been reported to result in higher polymerase activity in mouse-adapted strains of the 2009 pandemic influenza A H1N1 virus. We modeled wild and mutant complexes of uridine 5'-monophosphate (UMP) as the endonuclease substrate and performed molecular dynamics simulations. The results demonstrated that the F35L mutation could result in a changed orientation of a helix containing active site residues and improve the ligand affinity in the mutant strain...
December 22, 2017: Archives of Virology
https://www.readbyqxmd.com/read/29214233/supramolecular-assembly-of-uridine-monophosphate-ump-and-thymidine-monophosphate-tmp-with-a-dinuclear-copper-ii-receptor
#3
Md Mhahabubur Rhaman, Douglas R Powell, Md Alamgir Hossain
Understanding the intermolecular interactions between nucleotides and artificial receptors is crucial to understanding the role of nucleic acids in living systems. However, direct structural evidence showing precise interactions and bonding features of a nucleoside monophosphate (NMP) with a macrocycle-based synthetic molecule has not been provided so far. Herein, we present two novel crystal structures of uridine monophosphate (UMP) and thymidine monophosphate (TMP) complexes with a macrocycle-based dinuclear receptor...
November 30, 2017: ACS Omega
https://www.readbyqxmd.com/read/29205045/colon-ascendens-stent-peritonitis-casp-induces-excessive-inflammation-and-systemic-metabolic-dysfunction-in-a-septic-rat-model
#4
Limin Zhang, Yuan Tian, Jianfen Yang, Jieshou Li, Huiru Tang, Yulan Wang
The colon ascendens stent peritonitis (CASP) surgery creates a leakage of intestinal contents, resulting in polymicrobial sepsis associated with a high risk for postoperative multiple organ failure and death in surgical patients. To evaluate the effects of CASP on multiple organs, we analyzed the systemic metabolic consequences in liver, kidney, lung and heart of rats after CASP by employing a combination of metabolomics, clinical chemistry and biological assays. We found that CASP surgery after 18 h caused significant elevations of lipid, amino acids, acetate, choline, PC and GPC in rat liver together with significant depletion of glucose and glycogen...
December 5, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29187207/metabolomic-effects-of-ceo2-sio2-and-cuo-metal-oxide-nanomaterials-on-hepg2-cells
#5
Kirk T Kitchin, Steve Stirdivant, Brian L Robinette, Benjamin T Castellon, Xinhua Liang
BACKGROUND: To better assess potential hepatotoxicity of nanomaterials, human liver HepG2 cells were exposed for 3 days to five different CeO2 (either 30 or 100 μg/ml), 3 SiO2 based (30 μg/ml) or 1 CuO (3 μg/ml) nanomaterials with dry primary particle sizes ranging from 15 to 213 nm. Metabolomic assessment of exposed cells was then performed using four mass spectroscopy dependent platforms (LC and GC), finding 344 biochemicals. RESULTS: Four CeO2, 1 SiO2 and 1 CuO nanomaterials increased hepatocyte concentrations of many lipids, particularly free fatty acids and monoacylglycerols but only CuO elevated lysolipids and sphingolipids...
November 29, 2017: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/29176591/identifying-metabolites-by-integrating-metabolome-databases-with-mass-spectrometry-cheminformatics
#6
Zijuan Lai, Hiroshi Tsugawa, Gert Wohlgemuth, Sajjan Mehta, Matthew Mueller, Yuxuan Zheng, Atsushi Ogiwara, John Meissen, Megan Showalter, Kohei Takeuchi, Tobias Kind, Peter Beal, Masanori Arita, Oliver Fiehn
Novel metabolites distinct from canonical pathways can be identified through the integration of three cheminformatics tools: BinVestigate, which queries the BinBase gas chromatography-mass spectrometry (GC-MS) metabolome database to match unknowns with biological metadata across over 110,000 samples; MS-DIAL 2.0, a software tool for chromatographic deconvolution of high-resolution GC-MS or liquid chromatography-mass spectrometry (LC-MS); and MS-FINDER 2.0, a structure-elucidation program that uses a combination of 14 metabolome databases in addition to an enzyme promiscuity library...
November 27, 2017: Nature Methods
https://www.readbyqxmd.com/read/29174536/monophosphorylation-by-deoxycytidine-kinase-affects-apparent-cellular-uptake-of-decitabine-in-hct116-colon-cancer-cells
#7
Kumiko Ueda, Ayasa Masuda, Misaki Fukuda, Shota Tanaka, Mika Hosokawa, Seigo Iwakawa
Decitabine (DAC), a nucleoside-related DNA methylation inhibitor, is taken up into cancer cells via equilibrative nucleoside transporter 1 (ENT1), and is then monophosphorylated by deoxycytidine kinase (dCK). In the present study, we examined the contribution of dCK to the uptake of DAC in HCT116 colon cancer cells. Irinotecan and etoposide inhibited the uptake of [3H]-uridine and [3H]-DAC at 10 s and 5 min, while cytarabine and gemcitabine only inhibited that of [3H]-DAC at 5 min. Irinotecan and etoposide inhibited [3H]-DAC uptake in negative control small interfering RNA (siRNA)- or dCK siRNA-transfected cells at 10 s, whereas cytarabine and gemcitabine did not...
October 10, 2017: Drug Metabolism and Pharmacokinetics
https://www.readbyqxmd.com/read/29160924/accelerated-untargeted-metabolomics-analysis-of-cutaneous-t-cell-lymphoma-reveal-metabolic-shifts-in-plasma-and-tumor-adjacent-skins-of-xenograft-mice
#8
Yunchen Le, Xiaoyan Shen, Hongyan Kang, Qizheng Wang, Kejia Li, Jie Zheng, Yunqiu Yu
Cutaneous T-cell lymphoma (CTCL) is a heterogeneous group of skin-homing T-cell neoplasms. Clinical management is stage based but diagnosis and prognosis could be extremely challenging. The presented study aims to explore the metabolic profiling of CTCL by an accelerated untargeted metabolomics data analysis tool "mummichog" to facilitate the discoveries of potential biomarkers for clinical early-stage diagnosis, prognosis and treatments in CTCL. Ultra-high-performance liquid chromatography-quadrupole time-of-flight (UHPLC-QTOF) based untargeted metabolomics were conducted on the skin and plasma of CTCL mice...
November 21, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/29124970/a-review-on-the-nucleic-acid-constituents-in-mushrooms-nucleobases-nucleosides-and-nucleotides
#9
Chia-Wei Phan, Joon-Keong Wang, Shiau-Chuen Cheah, Murali Naidu, Pamela David, Vikineswary Sabaratnam
Mushrooms have become increasingly important as a reliable food source. They have also been recognized as an important source of bioactive compounds of high nutritional and medicinal values. The nucleobases, nucleosides and nucleotides found in mushrooms play important roles in the regulation of various physiological processes in the human body via the purinergic and/or pyrimidine receptors. Cordycepin, a 3'-deoxyadenosine found in Cordyceps sinensis has received much attention as it possesses many medicinal values including anticancer properties...
November 10, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/29099122/the-intrinsic-basicity-of-the-phosphate-backbone-exceeds-that-of-uracil-and-thymine-residues-protonation-of-the-phosphate-moiety-is-preferred-over-the-nucleobase-for-pdthd-and-purd
#10
R R Wu, L A Hamlow, C C He, Y-W Nei, G Berden, J Oomens, M T Rodgers
The gas-phase conformations of the protonated forms of thymidine-5'-monophosphate and uridine-5'-monophosphate, [pdThd+H](+) and [pUrd+H](+), are investigated by infrared multiple photon dissociation (IRMPD) action spectroscopy and electronic structure calculations. The IRMPD action spectra of [pdThd+H](+) and [pUrd+H](+) are measured over the IR fingerprint and hydrogen-stretching regions using the FELIX free electron laser and an OPO/OPA laser system. Low-energy conformations of [pdThd+H](+) and [pUrd+H](+) and their relative stabilities are computed at the MP2(full)/6-311+G(2d,2p)//B3LYP/6-311+G(d,p) and B3LYP/6-311+G(2d,2p)//B3LYP/6-311+G(d,p) levels of theory...
November 22, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29090559/-liver-metabonomics-of-acute-gouty-arthritis-treated-by-diosocorea-nipponica
#11
Shu-Min Liu, Ning Zhang, Dong-Hua Yu, Yu Wang, Qi Zhou, Fang Lu
To explore the prevention and protection effect of Diosocorea nipponica (DNM)) on acute gouty arthritis (AGA) rats based on liver metabonomics, and find potential biomarkers and related pathways. AGA model rats were induced by monosodium urate crystal suspension. UPLC-TOF-MS coupled with pattern recognition technique was employed to find out the potential biomarkers and related metabolic pathways. Eleven common potential biomarkers were identified. Among the potential intervention targets in normal rats given by DNM, 4 biomarkers were up-regulated, and the other 4 targets were down regulated...
May 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29048817/-determination-of-five-nucleotides-in-infant-formula-milk-powder-by-modified-high-performance-liquid-chromatography
#12
Jiong Li, Jiaofei Wang, Hongyu Qiu, Wei Li
A modified high performance liquid chromatographic (HPLC) method was developed for the determination of the five nucleotides (uridine monophosphate (UMP), adenosine monophosphate (AMP), inosine monophosphate (IMP), guanosine monophosphate (GMP) and cytidine monophosphate (CMP)) in infant formula milk powder. The samples were extracted by water, deproteinized by acetic acid and purified with an HLB SPE cartridge. The analytes were separated by a Waters XBrigde Amide column (150 mm×4.6 mm, 3.5 μ m). Acetonitrile, 10 mmol/L sodium dihydrogen phosphate aqueous solution and 0...
August 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29023666/nutritional-intervention-and-neurodevelopmental-outcome-in-infants-with-suspected-cerebral-palsy-the-dolphin-infant-double-blind-randomized-controlled-trial
#13
Morag J Andrew, Jeremy R Parr, Christine Montague-Johnson, Karen Laler, Cathy Qi, Bonny Baker, Peter B Sullivan
AIM: To investigate whether docosahexaenoic acid (DHA), choline, and uridine-5-monophosphate (UMP) supplementation improves neurodevelopmental outcome in infants with suspected cerebral palsy (CP) versus a comparison group of children. METHOD: Infants aged 1 to 18 months with suspected CP were recruited from UK child development centres. Participants received daily treatment or control supplementation for 2 years (double-blind randomized control design). Stratification was by age, sex, predominant pattern of motor involvement (four limbs or other), and visual impairment (or not)...
October 12, 2017: Developmental Medicine and Child Neurology
https://www.readbyqxmd.com/read/28835346/the-discovery-of-idx21437-design-synthesis-and-antiviral-evaluation-of-2-%C3%AE-chloro-2-%C3%AE-c-methyl-branched-uridine-pronucleotides-as-potent-liver-targeted-hcv-polymerase-inhibitors
#14
François-René Alexandre, Eric Badaroux, John P Bilello, Stéphanie Bot, Tony Bouisset, Guillaume Brandt, Sylvie Cappelle, Christopher Chapron, Dominique Chaves, Thierry Convard, Clément Counor, Daniel Da Costa, David Dukhan, Marion Gay, Gilles Gosselin, Jean-François Griffon, Kusum Gupta, Brenda Hernandez-Santiago, Massimiliano La Colla, Marie-Pierre Lioure, Julien Milhau, Jean-Laurent Paparin, Jérôme Peyronnet, Christophe Parsy, Claire Pierra Rouvière, Houcine Rahali, Rachid Rahali, Aurélien Salanson, Maria Seifer, Ilaria Serra, David Standring, Dominique Surleraux, Cyril B Dousson
Herein we describe the discovery of IDX21437 35b, a novel RPd-aminoacid-based phosphoramidate prodrug of 2'-α-chloro-2'-β-C-methyluridine monophosphate. Its corresponding triphosphate 6 is a potent inhibitor of the HCV NS5B RNA-dependent RNA polymerase (RdRp). Despite showing very weak activity in the in vitro Huh-7 cell based HCV replicon assay, 35b demonstrated high levels of active triphosphate 6 in mouse liver and human hepatocytes. A biochemical study revealed that the metabolism of 35b was mainly attributed to carboxyesterase 1 (CES1), an enzyme which is underexpressed in HCV Huh-7-derived replicon cells...
September 15, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28762967/multi-temperature-study-of-potassium-uridine-5-monophosphate-electron-density-distribution-and-anharmonic-motion-modelling
#15
Katarzyna N Jarzembska, Katarzyna Řlepokura, Radosław Kamiński, Matthias J Gutmann, Paulina M Dominiak, Krzysztof Woźniak
Uridine, a nucleoside formed of a uracil fragment attached to a ribose ring via a β-N1-glycosidic bond, is one of the four basic components of ribonucleic acid. Here a new anhydrous structure and experimental charge density distribution analysis of a uridine-5'-monophosphate potassium salt, K(UMPH), is reported. The studied case constitutes the very first structure of a 5'-nucleotide potassium salt according to the Cambridge Structural Database. The excellent crystal quality allowed the collection of charge density data at various temperatures, i...
August 1, 2017: Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials
https://www.readbyqxmd.com/read/28713420/modeling-the-metabolism-of-arabidopsis-thaliana-application-of-network-decomposition-and-network-reduction-in-the-context-of-petri-nets
#16
Ina Koch, Joachim Nöthen, Enrico Schleiff
Motivation:Arabidopsis thaliana is a well-established model system for the analysis of the basic physiological and metabolic pathways of plants. Nevertheless, the system is not yet fully understood, although many mechanisms are described, and information for many processes exists. However, the combination and interpretation of the large amount of biological data remain a big challenge, not only because data sets for metabolic paths are still incomplete. Moreover, they are often inconsistent, because they are coming from different experiments of various scales, regarding, for example, accuracy and/or significance...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28559281/uridine-monophosphate-synthetase-enables-eukaryotic-de-novo-nad-biosynthesis-from-quinolinic-acid
#17
Melanie R McReynolds, Wenqing Wang, Lauren M Holleran, Wendy Hanna-Rose
NAD(+) biosynthesis is an attractive and promising therapeutic target for influencing health span and obesity-related phenotypes as well as tumor growth. Full and effective use of this target for therapeutic benefit requires a complete understanding of NAD(+) biosynthetic pathways. Here, we report a previously unrecognized role for a conserved phosphoribosyltransferase in NAD(+) biosynthesis. Because a required quinolinic acid phosphoribosyltransferase (QPRTase) is not encoded in its genome, Caenorhabditis elegans are reported to lack a de novo NAD(+) biosynthetic pathway...
July 7, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28542039/role-of-the-uridine-cytidine-kinase-2-mutation-in-cellular-sensitiveness-toward-3-ethynylcytidine-treatment-of-human-cancer-cells
#18
Akira Sato, Takeshi Takano, Akiko Hiramoto, Tomoharu Naito, Akira Matsuda, Masakazu Fukushima, Yusuke Wataya, Hye-Sook Kim
A nucleosidic medicine, 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine [3'-ethynylcytidine (ECyd)], is a potent inhibitor of RNA polymerase I and shows anticancer activity to various human solid tumors in vitro and in vivo. ECyd is phosphorylated to 3'-ethyntlcytidine 5'-monophosphate by uridine/cytidine kinase 2 (UCK2) and subsequently further to diphosphate and triphosphate (3'-ethyntlcytidine 5'-diphosphate, 3'-ethyntlcytidine 5'-triphosphate). 3'-Ethyntlcytidine 5'-triphosphate is an active metabolite that can inhibit RNA polymerase I competitively, causing cancer cell death...
August 2017: Anti-cancer Drugs
https://www.readbyqxmd.com/read/28520982/genome-wide-mrna-processing-in-methanogenic-archaea-reveals-post-transcriptional-regulation-of-ribosomal-protein-synthesis
#19
Lei Qi, Lei Yue, Deqin Feng, Fengxia Qi, Jie Li, Xiuzhu Dong
Unlike stable RNAs that require processing for maturation, prokaryotic cellular mRNAs generally follow an 'all-or-none' pattern. Herein, we used a 5΄ monophosphate transcript sequencing (5΄P-seq) that specifically captured the 5΄-end of processed transcripts and mapped the genome-wide RNA processing sites (PSSs) in a methanogenic archaeon. Following statistical analysis and stringent filtration, we identified 1429 PSSs, among which 23.5% and 5.4% were located in 5΄ untranslated region (uPSS) and intergenic region (iPSS), respectively...
July 7, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28472077/improvement-of-uridine-production-of-bacillus-subtilis-by-atmospheric-and-room-temperature-plasma-mutagenesis-and-high-throughput-screening
#20
Xiaoguang Fan, Heyun Wu, Guoliang Li, Hui Yuan, Hongchao Zhang, Yanjun Li, Xixian Xie, Ning Chen
In the present study, a novel breeding strategy of atmospheric and room temperature plasma (ARTP) mutagenesis was used to improve the uridine production of engineered Bacillus subtilis TD12np. A high-throughput screening method was established using both resistant plates and 96-well microplates to select the ideal mutants with diverse phenotypes. Mutant F126 accumulated 5.7 and 30.3 g/L uridine after 30 h in shake-flask and 48 h in fed-batch fermentation, respectively, which represented a 4.4- and 8.7-fold increase over the parent strain...
2017: PloS One
keyword
keyword
116950
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"