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Panat Anuracpreeda, Amaya Watthanadirek, Runglawan Chawengkirttikul, Prasert Sobhon
A number of monoclonal antibodies (MoAbs) against the 16 kDa antigen of Paramphistomum gracile (16 kDaAgPg) were produced in vitro by hybridoma technique. Reactivity and specificity of these MoAbs were evaluated by ELISA and immunoblotting assays. Seven MoAb clones were selected from the stable hybridoma clones, namely 1D10, 2D7, 3B10, 3D9, 4F1, 4G4, and 5G12. It was found to be IgM and kappa light chain isotypes. By immunoblotting and ELISA, all MoAbs reacted with purified 16 kDaAgPg at molecular weight (MW) of 16 kDa and with the native 16 kDa antigen at MW of 16 kDa in the whole body (WB) and excretory-secretory (ES) fractions, but not with tegumental antigens (TA) of adult fluke...
October 22, 2016: Parasitology Research
Julia Angélica Gonçalves Silveira, Mirella Lauria D'Elia, Isabela de Oliveira Avelar, Lara Ribeiro de Almeida, Hudson Andrade Dos Santos, Danielle Ferreira de Magalhães Soares, Múcio Flávio Barbosa Ribeiro, Walter Dos Santos Lima, Roselene Ecco
An adult free-ranged female maned wolf was rescued from a periurban area subject to anthropogenic disturbances in the Minas Gerais, Brazil. The animal presented poor body condition and anemia. The clinical condition rapidly deteriorated culminating in dead and a necropsy was performed. The main gross lesions were marked anemia and blood content in the intestines accompanied by many types of parasites. The protozoa Rangelia vitalii was identified by histopathological analysis predominantly within the cytoplasm of endothelial cells of capillaries of the small intestine...
December 2016: International Journal for Parasitology. Parasites and Wildlife
Anders Dige, Tue Kruse Rasmussen, Peter Nejsum, Rikke Hagemann-Madsen, Andrew R Williams, Jørgen Agnholt, Jens F Dahlerup, Christian L Hvas
Helminthic therapy of immune-mediated diseases has gained attention in recent years, but we know little of how helminths modulate human immunity. In this study, we investigated how self-infection with Trichuris (T.) trichiura in an adult man without intestinal disease affected mucosal and systemic immunity. Colonic mucosal biopsies were obtained at baseline, during T. trichiura infection, and after its clearance following mebendazole treatment. Unexpectedly, the volunteer experienced a Campylobacter colitis following T...
October 15, 2016: Parasite Immunology
Whitni K Redman, Jay E Bryant, Gul Ahmad
AIM: This survey was carried out on the carcasses of 29 coyotes from Southeastern Nebraska and Shenandoah area of Iowa to document the helminths present in the intestinal track of these carnivorous animals. MATERIALS AND METHODS: A total of 29 adult coyote carcasses were generously donated in the autumn and winter (November-February) of 2014-2015 by trappers, fur buyers and hunters of Southeast Nebraska and Shenandoah area of Iowa. The intestine of individual animals were examined for the recovery of helminth parasites as per the established procedures...
September 2016: Veterinary World
Samuel Fuhrimann, Mirko S Winkler, Phuc Pham-Duc, Dung Do-Trung, Christian Schindler, Jürg Utzinger, Guéladio Cissé
BACKGROUND: Infections with intestinal parasites (helminths and intestinal protozoa) are endemic in Southeast Asia and inappropriate management and reuse of wastewater might exacerbate the risk of human infections. In rapidly growing urban settings, little is known about the extent of intestinal parasite infections. We assessed the point-prevalence and risk factors of intestinal parasite infections in population groups differently exposed to wastewater in urban and peri-urban transition zones in Hanoi, the capital of Vietnam...
October 10, 2016: Parasites & Vectors
Jürgen Schölmerich, Klaus Fellermann, Frank W Seibold, Gerhard Rogler, Jost Langhorst, Stefanie Howaldt, Gottfried Novacek, Andreas Munk Petersen, Oliver Bachmann, Harald Matthes, Norbert Hesselbarth, Niels Teich, Jan Wehkamp, Jochen Klaus, Claudia Ott, Karin Dilger, Roland Greinwald, Ralph Mueller
BACKGROUND & AIMS: To investigate the efficacy and safety of three different dosages of embryonated, viable eggs of Trichuris suis (TSO) versus placebo for induction of remission in mildly-to-moderately active ileocolonic, uncomplicated Crohn's disease (CD). METHODS: Adults with active CD (n=252) randomly received six fortnightly doses of 250, 2500 or 7500 TSO/15 ml suspension/day (TSO 250, TSO 2500, TSO 7500), or 15ml placebo solution/day, in a double-blind fashion, with four weeks' follow-up...
October 5, 2016: Journal of Crohn's & Colitis
M Bagayan, D Zongo, A Oueda, H Sorgho, B Savadogo, F Drabo, A Ouedraogo, J N Poda, B G Kabre, Y Zhang
To determine the current status of schistosomiasis and soil-transmitted helminth (STH) infections among schoolchildren after 10 years of mass treatment with praziquantel. Parasitological surveys were conducted in 2013 in 22 primary schools located in 11 regions of Burkina Faso. Urine filtration methods and Kato-Katz techniques for stool were used to detect the eggs of Schistosoma hæmatobium, Schistosoma mansoni, and STH. 3514 schoolchildren aged from 7 to 11 (1.415 ± 9) years participated in the study. The prevalence of S...
August 1, 2016: Médecine et Santé Tropicales
Jolanta Zdybel, Jacek Karamon, Mirosław Różycki, Ewa Bilska-Zając, Teresa Kłapeć, Tomasz Cencek
Because traditional methods used for sewage sludge parasitological examinations have low sensitivity, a new, highly effective method (own method - OM) was devised. The principle of this method is to eliminate the flocculent effect on the structure of sewage sludge by mechanically damaging floccules in the presence of surfactants and to increase the effectiveness of egg isolation processes in large volumes of liquids. The objective of this study was to estimate the effectiveness of the OM in detecting nematode eggs in sewage sludge samples containing flocculants...
September 28, 2016: Experimental Parasitology
Brandi T Wren, Melissa J Remis, Joseph W Camp, Thomas R Gillespie
There are many known benefits of social grooming among primates, including maintenance of social relationships, removal of ectoparasites, and improved physiological condition. Recently, however, researchers have noted that social grooming and social contact may also present a significant cost by facilitating transmission of some parasites and pathogens. We investigated whether the number of social grooming partners varied based on infection status for gastrointestinal parasites. We used focal animal sampling and continuous recording to collect data on the number of grooming partners for known individual vervet monkeys (Chlorocebus aethiops)...
September 29, 2016: Folia Primatologica; International Journal of Primatology
Giuseppina Ortu, Mohamad Assoum, Udo Wittmann, Sarah Knowles, Michelle Clements, Onésime Ndayishimiye, Maria-Gloria Basáñez, Colleen Lau, Archie Clements, Alan Fenwick, Ricardo J Soares Magalhaes
BACKGROUND: Soil-transmitted helminth (STH) infections are amongst the most prevalent infections in the world. Mass drug administration (MDA) programmes have become the most commonly used national interventions for endemic countries to achieve elimination. This paper aims to describe the effect of an 8-year MDA programme on the prevalence, intensity of infection and co-infection of STH in Burundi from 2007 to 2014 and critically appraise the trajectory towards STH elimination in the country...
2016: Parasites & Vectors
Bui Khac Hung, Nguyen Van De, Le Van Duyet, Jong-Yil Chai
To know the infection status of helminths in primary schoolchildren of southern parts of Vietnam, we performed an epidemiological study in Krong Pac district, Dak Lak Province, Vietnam. A total of 1,206 stool specimens were collected from ethnic Ede schoolchildren in 4 primary schools in 2015 and examined by the Kato-Katz technique. In addition, stool cultures were done by the Harada-Mori method to obtain hookworm larvae and then to clarify the species of hookworms infected. The results showed that the helminth infection rate was 25...
August 2016: Korean Journal of Parasitology
Pelagie M Boko, Moudachirou Ibikounle, Ablawa Onzo-Aboki, Jean-Jacques Tougoue, Yollande Sissinto, Wilfrid Batcho, Dorothe Kinde-Gazard, Achille Kabore
In 2013, Benin developed strategies to control neglected tropical diseases and one of the first step was the disease mapping of the entire country in order to identify endemic districts of schistosomiasis and soil transmitted helminths (STH). This study was carried out in 30 of the 77 districts of Benin. Of these 30 districts 22 were previously treated for Lymphatic Filariasis (LF) using the Ivermectin and Albendazole combination. In each district, five schools were selected and 50 children aged 8 to 14 years were sampled in each school, making a total of 250 children sampled in the district...
2016: PloS One
Fabienne Schubnell, Sereina von Ah, Robert Graage, Titus Sydler, Xaver Sidler, Daniela Hadorn, Walter Basso
In order to estimate the diversity, clinical involvement and zoonotic potential of parasites in pigs submitted for diagnosis to the PathoPig project of the Swiss Federal Food Safety and Veterinary Office, faeces (n=125) from suckling piglets (n=39), weaners (n=60) and piglets beginning fattening (n=26) from 74 Swiss farms were examined by 3 coproscopical methods (i.e. sedimentation/zinc chloride-flotation; SAFC and Ziehl-Neelsen staining). Samples microscopically positive for Cryptosporidium were further tested by PCR/sequencing for species assessment...
December 2016: Parasitology International
Steffen Rehbein, M Knaus, M Visser, R Rauh, S Yoon
Gastrointestinal and pulmonary nematode infections are affecting the health and productivity of grazing cattle worldwide. To evaluate the effects of a single treatment with ivermectin long-acting injection (IVM LAI; IVOMEC® GOLD, Merial; 3.15 % ivermectin w/v) in first-grazing season cattle, two studies were conducted under continued stocking conditions for 84 or 100 days in Bavaria, Germany. Each study involved 68 naturally infected, approximately 4- to 6-month-old Brown Swiss bull calves. Animals were blocked based on pretreatment body weights...
September 15, 2016: Parasitology Research
C Muñoz-Antoli, A Pavón, P Pérez, R Toledo, J G Esteban
Soil-transmitted helminth (STH) prevalence, intensity, polyparasitism and co-infections in 425 children from 3 schools of Laguna de Perlas (Nicaragua) were investigated. Single stool samples were analysed by the formalin-ether method and the Kato-Katz. A total of 402 (94.6%) children were infected. Trichuris trichiura 308 (72.4%), Ascaris lumbricoides 115 (27.1%) and Hookworms 54 (12.7%) were the most prevalent STHs. Polyparasitism (322; 75.8%) with two species was most prevalent (109; 25.6%). T. trichiura with A...
September 10, 2016: Journal of Tropical Pediatrics
Shahabeddin Sarvi, Ahmad Daryani, Mahdi Sharif, Mohammad Taghi Rahimi, Davood Azami, Zahra Marhaba, Ehsan Ahmadpour, Azadeh Mizani
An epidemiological study was conducted to determine the prevalence of helminthic parasites of domestic dogs using stool samples in north of Iran in 2013. Stool samples (n = 100) were collected from domestic dogs of different ages and genders. Different techniques including wet smear, formalin-ether sedimentation method and flotation technique in saturated zinc chloride solution were performed on collected stools to detect eggs and larval stage of helminthes parasites. The overall prevalence of helminthic parasites of examined stools of dogs was 57 %...
September 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
Bashir A Lone, M Z Chishti, Fayaz Ahmad, Hidayatullah Tak, Suhaib A Bandh, Abida Khan
One year crossectional survey was carried out to determine and describe the prevalence and intensity of gastrointestinal parasite infections in hangul (Cervus elaphus hanglu) in Dachigam National Park of Kashmir through faecal examinations. Out of 153 faecal samples examined, 82 (53.59 %) were found infected with GIT helminthes. In present study seven helminth species were found, including five nematode [Haemonchus contortus (55.39 %), Trichuris ovis (39.75 %), Dictyocaulus viviparus (28.4.00 %), Oesophogostomum circumcincta (13...
September 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
Nazima Gul, Hidayatullah Tak
The present study aims to determine the age- wise, sex- wise and month-wise prevalence along with seasonal fluctuations of Trichuris spp. in ovines and caprines slaughtered during a 12 month period in local abattoirs in Srinagar region from August 2011 to July 2012. Adult parasites were identified on the basis of morphological characters (Soulsby Helminths, arthropods and protozoa of domes- 229 ticated animals, CLBS and Bailliere Tinda, London,1982). The highest prevalence (66.6 %) was in the month of Jan, 2012 whereas prevalence was lowest in the month of August 2011...
September 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
Taís Rondello Bonatti, Regina Maura Bueno Franco
Biosolid is the product of the activated sludge treatment system and its final disposition is subject of ongoing discussion as this residue can therefore harbor a great number and variety of pathogens. This study was aimed to (1) monitor the presence of Giardia and Cryptosporidium in biosolid samples from a treatment plant in Campinas, SP, Brazil, (2) observe Giardia cyst wall morphological integrity in treated samples using scanning electron microscopy (SEM) and (3) verify the presence and viability of helminth eggs...
September 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
C M Krishna Murthy, Placid E D' Souza
The study was undertaken to know the current status of prevalence of gastrointestinal parasites of cattle and buffaloes in Bangalore, Karnataka. An overall prevalence of gastrointestinal parasites among cattle (75.2 %) and buffalos (76.8 %) was determined by coprological examination. The gastrointestinal parasites detected in cattle and buffalo were Strongyle (39.8 and 29.1 %), followed by Amphistome (24.4 and 23.1 %), Moniezia spp. (5.3 and 5.9 %), Fasciola spp. (4.1 and 15.6 %), Trichuris spp. (1.4 and 2...
September 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
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