Read by QxMD icon Read


Ruo Dan Liu, Xin Qi, Ge Ge Sun, Peng Jiang, Xi Zhang, Li Ang Wang, Xiao Lin Liu, Zhong Quan Wang, Jing Cui
At the intestinal stage of a Trichinella spiralis (T. spiralis) infection, the excretory-secretory (ES) antigens produced by adult worms (AWs) result in an early exposure to the host's immune system and elicit the production of specific antibodies; the AW ES proteins might provide early diagnostic markers of trichinellosis. The aim of this study was to identify early serodiagnostic markers from T. spiralis AW ES antigens. T. spiralis AWs were collected at 72h post infection, and their ES antigens were analysed by SDS-PAGE and Western blot...
October 11, 2016: Veterinary Parasitology
Miriam Maas, Sanne van den End, Annika van Roon, Jaap Mulder, Frits Franssen, Cecile Dam-Deisz, Margriet Montizaan, Joke van der Giessen
The recent invasion of the raccoon dog in the Netherlands may be associated with the risk of introduction and spread of zoonotic pathogens. The aim of this study was to assess whether Echinococcus multilocularis and Trichinella spp. infections are present in Dutch raccoon dogs. Between 2013 and 2014, nine raccoon dogs, mainly road kills, were collected for necropsies. One raccoon dog tested repeatedly positive in the qPCR for E. multilocularis. The positive raccoon dog was collected in the province of Flevoland, which is not a known endemic region for E...
December 2016: International Journal for Parasitology. Parasites and Wildlife
José Luis Muñoz-Carrillo, José Jesús Muñoz-Escobedo, Claudia H Maldonado-Tapia, Francisca Chávez-Ruvalcaba, María Alejandra Moreno-García
During the course of infection with Trichinella spiralis, an inflammatory response is triggered at the intestinal level in the host, playing a crucial role in the expulsion and elimination of the parasite. However, several studies have demonstrated that this inflammatory response is harmful to the host; hence the importance of studying molecules with therapeutic potential like resiniferatoxin, which is known to have an anti-inflammatory effect both in-vitro and in-vivo. In this article, we evaluated the anti-inflammatory activity of resiniferatoxin during the intestinal phase of T...
October 15, 2016: Parasite Immunology
Kazunobu Asano, Zhiliang Wu, Piyarat Srinontong, Takahide Ikeda, Isao Nagano, Hirokuyi Morita, Yoichi Maekawa
Infectious microorganisms often modify host immunity to escape from immune elimination. Trichinella is a unique nematode of the helminth family, which parasitize the inside of host muscle cells without robust eliminative reactions. There are two main species in genus Trichinella, encapsulated (T. spiralis(Ts), T. britovi) and non-encapsulated (T. pseudospiralis(Tp)). It has already been established that Trichinella infection affects host immune responses in several experimental immune diseases in animal models; however, most of those studies were done using Ts infection...
October 10, 2016: Infection and Immunity
Brian Lassen, Marilin Janson, Arvo Viltrop, Kädi Neare, Pirje Hütt, Irina Golovljova, Lea Tummeleht, Pikka Jokelainen
We investigated Estonian population and its selected subgroups for serological evidence of exposure to Ascaris lumbricoides, Echinococcus spp., Taenia solium, Toxocara canis, Toxoplasma gondii, and Trichinella spiralis. Serum samples from 999 adults representing general population, 248 children aged 14-18, 158 veterinarians, 375 animal caretakers, and 144 hunters were tested for specific immunoglobulin G antibodies against the selected parasites using commercial enzyme immunoassays (ELISA). Sera yielding positive or twice grey zone Echinococcus spp, T...
2016: PloS One
Liang Ming, Ruo Yu Peng, Lei Zhang, Chun Li Zhang, Pin Lv, Zhong Quan Wang, Jing Cui, Hui Jun Ren
As we all know, invasion of host intestinal epithelium is very important for T. spiralis to complete successfully their life cycle. However, the mechanisms that the intestinal infective larvae (IIL) invade and migrate in the intestinal epithailial cells (IECs) remain unclear until now. The related researches have been hindered since a readily operable in vitro normal model. In our earlier study, an in vitro normal IEC invasion model was established for the first time, and the abilities of the normal IECs to initiate mucosal inflammatory responses to invasion by the IIL in vitro were evaluated in this study...
October 4, 2016: Experimental Parasitology
Aleksandra Cybulska, Aleksandra Kornacka, Justyna Bień, Katarzyna Goździk, Elżbieta Kalisińska, Natalia Łanocha-Arendarczyk, Halina Budis, Bogumiła Pilarczyk, Władysław Cabaj, Bożena Moskwa
Trichinellosis is one of the most widespread parasitic zoonoses. Trichinella Owen, 1835 nematodes are found in pigs, horses, and humans in the domestic cycle, and in many carnivores and omnivores in the sylvatic cycle, such as wild boars, red foxes, raccoon dogs, and wolves. Carnivores are known to be involved in the circulation of Trichinella nematodes and they act as a reservoir in the sylvatic environment. The aim of this study was to determine the occurrence of Trichinella spp. infection in red foxes in Poland...
October 5, 2016: Vector Borne and Zoonotic Diseases
Peter Messiaen, Annemie Forier, Steven Vanderschueren, Caroline Theunissen, Jochen Nijs, Marjan Van Esbroeck, Emmanuel Bottieau, Koen De Schrijver, Inge C Gyssens, Reinoud Cartuyvels, Pierre Dorny, Jeroen van der Hilst, Daniel Blockmans
Trichinellosis is a rare parasitic zoonosis caused by Trichinella following ingestion of raw or undercooked meat containing Trichinella larvae. In the past five years, there has been a sharp decrease in human trichinellosis incidence rates in the European Union due to better practices in rearing domestic animals and control measures in slaughterhouses. In November 2014, a large outbreak of trichinellosis occurred in Belgium, related to the consumption of imported wild boar meat. After a swift local public health response, 16 cases were identified and diagnosed with trichinellosis...
September 15, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
M Miterpáková, D Antolová, Z Hurníková, N Březinová, V Čabanová, K Reiterová
Trichinellosis is constantly present in the sylvatic cycle in Slovakia, with several sporadic human outbreaks registered since the 1960s. The largest outbreak of trichinellosis occurred in 1998 and was related to the consumption of dog meat that had been added to smoked pork sausages. The aim of this study was to estimate the seroprevalence of Trichinella infection in domestic dogs in various regions of Slovakia. Out of 439 dogs tested, 56 (12.8%) were classified as a seropositive based on the enzyme-linked immunosorbent assay (ELISA), with confirmation by Western blotting...
September 29, 2016: Journal of Helminthology
Peng Jiang, Xi Zhang, Li Ang Wang, Lu Hong Han, Mei Yang, Jiang Yang Duan, Ge Ge Sun, Xin Qi, Ruo Dan Liu, Zhong Quan Wang, Jing Cui
The aim of this work was to investigate the current situation of Trichinella infection from domestic pigs in the historical endemic areas of Henan province, central China. A total of 823 diaphragm samples from the indoor-raised pigs were collected in five cities of Henan during 2014-2015 and examined by artificial digestion method. The overall prevalence of Trichinella infection in pigs was 0.61 % (5/823). Trichinella larvae were detected in 0.91 % (5/550) of pigs from Nanyang city of Henan. The larval burden in infected animals was 0...
September 7, 2016: Parasitology Research
Magdalena Dabrowska, Marek Skoneczny, Zbigniew Zielinski, Wojciech Rode
BACKGROUND: The nurse cell (NC) constitutes in mammalian skeletal muscles a confined intracellular niche to support the metabolic needs of muscle larvae of Trichinella spp. encapsulating species. The main biological functions of NC were identified as hypermitogenic growth arrest and pro-inflammatory phenotype, both inferred to depend on AP-1 (activator protein 1) transcription factor. Since those functions, as well as AP-1 activity, are known to be regulated among other pathways, also by Wnt (Wingless-Type of Mouse Mammary Tumor Virus Integration Site) signaling, transcription profiling of molecules participating in Wnt signaling cascades in NC, was performed...
2016: Parasites & Vectors
Lei Wang, Xiaohuan Wang, Kuo Bi, Ximeng Sun, Jing Yang, Yuan Gu, Jingjing Huang, Bin Zhan, Xinping Zhu
BACKGROUND: Our previous studies showed that Trichinella spiralis paramyosin (TsPmy) is an immunomodulatory protein that inhibits complement C1q and C8/C9 to evade host complement attack. Vaccination with recombinant TsPmy protein induced protective immunity against T. spiralis larval challenge. Due to the difficulty in producing TsPmy as a soluble recombinant protein, we prepared a DNA vaccine as an alternative approach in order to elicit a robust immunity against Trichinella infection...
September 2016: PLoS Neglected Tropical Diseases
Dalia S Ashour, Dina M Abou Rayia, Abeer E Saad, Reda H El-Bakary
Most of the drugs used for the treatment of trichinellosis show a limited bioavailability and a high degree of resistance. Therefore, this study aimed to characterize the anthelmintic potential activity of nitazoxanide (NTZ) in a rat model of experimental trichinellosis. Animals were divided into three groups; group I, infected and non-treated; group II, received NTZ for three days post-infection (dpi) and group III, received NTZ 30 dpi for 14 consecutive days. Treatment efficacy was assessed by Trichinella spiralis adult and larval counts, histopathological studies of the small intestine and muscles and inducible nitric oxide synthase (iNOS) expression in the small intestine...
August 30, 2016: Experimental Parasitology
M V Reichard, K Logan, M Criffield, J E Thomas, J M Paritte, D M Messerly, M Interisano, G Marucci, E Pozio
Trichinella species are zoonotic nematodes that infect wild carnivores and omnivores throughout the world. We examined the prevalence and species of Trichinella infections in cougars (Puma concolor couguar) from Colorado, United States. Tongues from cougars were examined by pepsin-HCl artificial digestion to detect Trichinella spp. larvae. The species or genotype of individual worms was identified by multiplex polymerase chain reaction (PCR). Trichinella spp. larvae were detected in 17 of 39 cougars (43.6% (28...
May 6, 2016: Journal of Helminthology
Takeshi Hatta, Kenkichi Imamura, Takehisa Yamamoto, Makoto Matsubayashi, Naotoshi Tsuji, Toshiyuki Tsutsui
This study presents the results of a large-scale one-year survey of Trichinella spp. in Japanese wild boar (Sus scrofa). We analyzed the tongues of 1168 wild boars captured by hunters in 30 prefectures of Japan, most of which were boar habitats, from October 2014 to January 2015. The samples were digested, and the Trichinella spp. muscle larvae prevalences were examined. Pooled samples from 10 individuals (15 g per head) and 117 randomly selected samples (10% of the total samples) that were processed individually showed no larval infection...
August 31, 2016: Japanese Journal of Infectious Diseases
Hui-Hui Li, Wen-Xin He, Di Song, Qi Wu, Nan Li, Yong-Kun Wan, Hui Zhang, Da-Peng Qiu, Liang Chu, Li-Yuan Wang, Xiao-di Yang, Qiang Fang
OBJECTIVE: To observe the effect of Trichinella spiralis and its worm-derived proteins on cecal ligation and puncture (CLP)-induced sepsis in mice. METHODS: Eighty male BALB/c mice were randomly divided into sham-operated group, CLP group, Trichinella spiralis muscle larvae (ML) pre-infection group (ML+CLP group), soluble muscle larvae proteins (SMP) treatment group (SMP+CLP group) and excretory-secretory proteins (MES) treatment group (MES+CLP group). In ML+CLP group, the mice were orally infected with 300 Trichinella spiralis muscle larvae at 28 days before CLP and those in the other groups were intraperitioneally injected with PBS or SMP (25 µg/mice) or MES (25µg/mice) 30 min after CLP...
August 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Irina M Odoevskaya, Sergei E Spiridonov
Partial nucleotide sequences of the two mitochondrial genes (cytB and COI) were obtained for 13 Trichinella isolates from different regions of the Russian Federation. All the cytB mtDNA sequences were identical with the sequences of T. nativa from Canada, showing no nucleotide differences between isolates. Analysis of partial COI mtDNA sequence confirmed the species identification and revealed differences between the studied isolates. Two T. nativa haplotypes, represented in the studied material by four samples, demonstrate limited geographical distribution...
August 16, 2016: Veterinary Parasitology
Szu-Wei Huang, Catherine Walker, Joanne Pennock, Kathryn Else, Werner Muller, Michael J D Daniels, Carolina Pellegrini, David Brough, Gloria Lopez-Castejon, Sheena M Cruickshank
Infection and injury of the gut are associated with cell damage and release of molecules such as extracellular ATP which is recognised by the purinergic P2X7 receptor (P2X7R). P2X7R is widely expressed in the gut by antigen presenting cells (APC) and epithelial cells, but the role of the P2X7R on epithelial cells is poorly understood. We investigated P2X7R in intestinal epithelium in vitro and in vivo using two model infections- Toxoplasma gondii and Trichinella spiralis. LPS and ATP treatment of intestinal epithelial cells and infection with T...
August 25, 2016: Immunology and Cell Biology
Everett K Henry, Chandler B Sy, Juan M Inclan-Rico, Vanessa Espinosa, Saleena S Ghanny, Daniel F Dwyer, Patricia Soteropoulos, Amariliz Rivera, Mark C Siracusa
Type 2 cytokine responses are necessary for the development of protective immunity to helminth parasites but also cause the inflammation associated with allergies and asthma. Recent studies have found that peripheral hematopoietic progenitor cells contribute to type 2 cytokine-mediated inflammation through their enhanced ability to develop into mast cells. In this study, we show that carbonic anhydrase (Car) enzymes are up-regulated in type 2-associated progenitor cells and demonstrate that Car enzyme inhibition is sufficient to prevent mouse mast cell responses and inflammation after Trichinella spiralis infection or the induction of food allergy-like disease...
August 22, 2016: Journal of Experimental Medicine
Pietro Badagliacca, Daria Di Sabatino, Stefania Salucci, Gianfranco Romeo, Micaela Cipriani, Nadia Sulli, Francesca Dall'Acqua, Marco Ruggieri, Paolo Calistri, Daniela Morelli
: During the period 2004-2014 in the Abruzzi region (Central Italy), muscle samples gathered from hunted wild boars (n=16,323) and retrieved from carcasses of other susceptible wild mammals (n=838) and birds (n=438) were tested for Trichinella larvae according to European Union regulations. Although no positive samples were found from wild birds, 91 wild mammals tested positive. Six species were found to harbor Trichinella spp. infections, namely wolf (Canis lupus, 59 positive samples out of 218), red fox (Vulpes vulpes, 24/480), wild boar (Sus scrofa, 3/16,323), stone marten (Martes foina, 2/27), pine marten (Martes martes, 2/6) and wildcat (Felis silvestris, 1/8)...
July 26, 2016: Veterinary Parasitology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"