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https://www.readbyqxmd.com/read/29232490/co2-to-terpenes-autotrophic-and-electroautotrophic-%C3%AE-humulene-production-with-cupriavidus-necator
#1
Thomas Krieg, Anne Sydow, Sonja Faust, Ina Huth, Dirk Holtmann
Herein, we demonstrate that CO2 can be converted by an engineered "Knallgas" bacterium (Cupriavidus necator) to the terpene α-humulene. Heterologous expression of the mevalonate pathway and α-humulene synthase resulted in a production of approx. 10 mg α-humulene per gram cell dry mass (CDM) under heterotrophic conditions. This first example of chemolithoautotrophic production of a terpene from carbon dioxide, hydrogen and oxygen is a promising starting point for the production of different high value terpene compounds from abundant and simple raw materials...
December 12, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29217186/biosynthesis-of-silver-nanoparticles-and-polyhydroxybutyrate-nanocomposites-of-interest-in-antimicrobial-applications
#2
J L Castro-Mayorga, F Freitas, M A M Reis, M A Prieto, J M Lagaron
This study deals with the optimization and scaling up of the production of poly(3-hydroxybutyrate), PHB, nanocomposites containing biosynthesized silver nanoparticles (AgNPs) to generate materials with antimicrobial performance. First, a comparative study of the chemical and biological synthesis of AgNPs during the fermentation process of Cupriavidus necator at shake flask-scale was carried out. These experiments demonstrated the inherent capacity of C. necator to reduce the silver salt and produce AgNPs without the need for adding a reducing agent and, that the method of synthesis (with or without reducing agent) affects the dispersion of the AgNPs and their antimicrobial performance...
December 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29216182/the-physicochemical-fingerprint-of-necator-americanus
#3
Veeren M Chauhan, David J Scurr, Thomas Christie, Gary Telford, Jonathan W Aylott, David I Pritchard
Necator americanus, a haematophagous hookworm parasite, infects ~10% of the world's population and is considered to be a significant public health risk. Its lifecycle has distinct stages, permitting its successful transit from the skin via the lungs (L3) to the intestinal tract (L4 maturing to adult). It has been hypothesised that the L3 larval sheath, which is shed during percutaneous infection (exsheathment), diverts the immune system to allow successful infection and reinfection in endemic areas. However, the physicochemical properties of the L3 larval cuticle and sheath, which are in direct contact with the skin and its immune defences, are unknown...
December 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29199089/applying-asymptotic-methods-to-synthetic-biology-modelling-the-reaction-kinetics-of-the-mevalonate-pathway
#4
Mohit P Dalwadi, Marco Garavaglia, Joseph P Webb, John R King, Nigel P Minton
The mevalonate pathway is normally found in eukaryotes, and allows for the production of isoprenoids, a useful class of organic compounds. This pathway has been successfully introduced to Escherichia coli, enabling a biosynthetic production route for many isoprenoids. In this paper, we develop and solve a mathematical model for the concentration of metabolites in the mevalonate pathway over time, accounting for the loss of acetyl-CoA to other metabolic pathways. Additionally, we successfully test our theoretical predictions experimentally by introducing part of the pathway into Cupriavidus necator...
November 30, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/29157661/analysis-of-codon-usage-pattern-of-mitochondrial-protein-coding-genes-in-different-hookworms
#5
Bornali Deb, Arif Uddin, Gulshana Akthar Mazumder, Supriyo Chakraborty
The phenomenon of unequal usage of synonymous codons encoding an amino acid in which some codons are more preferred to others is the codon usage bias (CUB) and it is species specific. Analysis of CUB helps in understanding evolution at molecular level and acquires significance in mRNA translation, design of transgenes and new gene discovery. In our current study, we analyzed synonymous codon usage pattern and the factors influencing it on mitochondrial protein coding genes of 6 different hookworms i.e. Ancylostoma ceylanicum, Ancylostoma duodenale, Necator americanus, Ancylostoma tubaeforme, Ancylostoma caninum and Uncinaria sanguinis as no work was reported yet...
November 17, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29114490/evaluation-of-the-utility-of-conventional-polymerase-chain-reaction-for-detection-and-species-differentiation-in-human-hookworm-infections
#6
Meenachi Chidambaram, Subhash Chandra Parija, Pampa Ch Toi, Jharna Mandal, Dhanalakshmi Sankaramoorthy, Santosh George, Mailan Natarajan, Shashiraja Padukone
Background: Human hookworm infection is caused mainly by Necator americanus and Ancylostoma duodenale. Among the zoonotic hookworm species, only Ancylostoma ceylanicum causes potent human infections where dogs and cats act as reservoir of infection. Hence, species differentiation is imperative because the eradication of both anthroponotic and zoonotic hookworm depends on the concurrent human and animal health programs, hygienic practices, and mass drug administration for humans and dogs...
July 2017: Tropical Parasitology
https://www.readbyqxmd.com/read/29114485/laboratory-diagnosis-of-soil-transmitted-helminthiasis
#7
Sumeeta Khurana, Shveta Sethi
Soil-transmitted helminths (STHs) include, i.e., hookworms (Ancylostoma duodenale, Necator americanus), roundworm (Ascaris lumbricoides), whipworm (Trichuris trichiura) and Strongyloides stercoralis. Globally, around 1.5 billion people are infected with STHs. STHs contribute to significant impairment of mental and physical growth, especially in developing countries. Unfortunately, these infections mostly remain undiagnosed due to lack of trained personnel and appropriate technologies. Intermittent shedding of eggs or larvae further makes the diagnosis difficult...
July 2017: Tropical Parasitology
https://www.readbyqxmd.com/read/29114484/epidemiology-and-clinical-features-of-soil-transmitted-helminths
#8
Subhash Chandra Parija, Meenachi Chidambaram, Jharna Mandal
Soil-transmitted helminths (STH) consist of Ascaris lumbricoides, Trichuris trichiura, and hookworm (Necator americanus and Ancylostoma duodenale). It affects nearly 1.7 billion people globally in which Ascaris contributes nearly 1.2 billion cases. The main mode for transmission of Ascaris and Trichuris is through contaminated food and water, whereas hookworm transmitted by skin penetration. STH were mainly seen in areas with poverty, overcrowding, and poor sanitation. The prevalence is more in rural areas compared to urban areas...
July 2017: Tropical Parasitology
https://www.readbyqxmd.com/read/29113820/biorefinery-production-of-poly-3-hydroxybutyrate-using-waste-office-paper-hydrolysate-as-feedstock-for-microbial-fermentation
#9
Annamalai Neelamegam, Huda Al-Battashi, Saif Al-Bahry, Sivakumar Nallusamy
Waste paper, a major fraction of municipal solid waste, has a potential to serve as renewable feedstock for the biorefineries of fuels, chemicals and materials due to rich in cellulose and abundant at low cost. This study evaluates the possibility of waste office paper (WOP) to serve as a potential feedstock for the biorefinery production of poly (3-hydroxybutyrate). In this study, the WOP was pretreated, enzymatically saccharified and the hydrolysate was used for PHB production. The hydrolysate mainly consists of glucose (22...
November 4, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/29108026/status-of-soil-transmitted-helminth-infections-in-schoolchildren-in-laguna-province-the-philippines-determined-by-parasitological-and-molecular-diagnostic-techniques
#10
Mary Lorraine S Mationg, Catherine A Gordon, Veronica L Tallo, Remigio M Olveda, Portia P Alday, Mark Donald C Reñosa, Franziska A Bieri, Gail M Williams, Archie C A Clements, Peter Steinmann, Kate Halton, Yuesheng Li, Donald P McManus, Darren J Gray
BACKGROUND: Soil-transmitted helminths (STH) are the most common parasitic infections in impoverished communities, particularly among children. Current STH control is through school-based mass drug administration (MDA), which in the Philippines is done twice annually. As expected, MDA has decreased the intensity and prevalence of STH over time. As a result, the common Kato Katz (KK) thick smear method of detecting STH is less effective because it lacks sensitivity in low intensity infections, making it difficult to measure the impact of deworming programs...
November 6, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29101024/an-integrative-study-on-biologically-recovered-polyhydroxyalkanoates-phas-and-simultaneous-assessment-of-gut-microbiome-in-yellow-mealworm
#11
Su Yean Ong, Hui-Pheng Kho, Sebastian L Riedel, Seok-Won Kim, Chee-Yuen Gan, Todd D Taylor, Kumar Sudesh
Polyhydroxyalkanoates (PHAs) are produced in microbes as a source of carbon and energy storage. They are biodegradable and have properties similar to synthetic plastics, which make them an interesting alternative to petroleum-based plastics. In this study, a refined method of recovering PHA from Cupriavidus necator biomass was proposed by incorporating the use of the yellow mealworm (the larval phase of the mealworm beetle, Tenebrio molitor) as partial purification machinery, followed by washing of the fecal pellets with distilled water and sodium hydroxide...
October 31, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/29054557/optimization-of-hexanoic-acid-production-in-recombinant-escherichia-coli-by-precise-flux-rebalancing
#12
Seong Gyeong Kim, Sungho Jang, Jae Hyung Lim, Byoung Seung Jeon, Jungyeon Kim, Kyoung Heon Kim, Byoung-In Sang, Gyoo Yeol Jung
The aim of this study is to demonstrate that rebalancing of metabolic fluxes at acetyl-CoA branch node can substantially improve the titer and productivity of hexanoic acid in recombinant Escherichia coli strains. First, a hexanoic acid-producing E. coli strain was constructed by expressing genes encoding β-ketothiolase (BktB) from Cupriavidus necator and acetyl-CoA transferase (ACT) from Megasphaera sp. MH in a butyric acid producer strain. Next, metabolic flux was optimized at the acetyl-CoA branch node by fine-tuning the expression level of the gene for acetyl-CoA acetyltransferase (AtoB)...
October 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29047387/isothermal-diagnostic-assays-for-the-detection-of-soil-transmitted-helminths-based-on-the-smartamp2-method
#13
Nour Rashwan, Aïssatou Diawara, Marilyn E Scott, Roger K Prichard
BACKGROUND: Diagnosis of soil-transmitted helminths (STHs) has traditionally relied on stool microscopy, which has a number of critical deficiencies. Molecular diagnostics are powerful tools to identify closely related species, but the requirement for costly equipment makes their implementation difficult in low-resource or field settings. Rapid, sensitive and cost-effective diagnostic tools are crucial for accurate estimation of STH infection intensity in MDA programmes in which the goal is to reduce morbidity following repeated rounds of chemotherapy...
October 18, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29022146/cupriavidus-malaysiensis-sp-nov-a-novel-poly-3-hydroxybutyrate-co-4-hydroxybutyrate-accumulating-bacterium-isolated-from-the-malaysian-environment
#14
Hema Ramachandran, Nur Asilla Hani Shafie, Kumar Sudesh, Mohamad Noor Azizan, Mohamad Isa Abdul Majid, Al-Ashraf Abdullah Amirul
Bacterial classification on the basis of a polyphasic approach was conducted on three poly(3 hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] accumulating bacterial strains that were isolated from samples collected from Malaysian environments; Kulim Lake, Sg. Pinang river and Sg. Manik paddy field. The Gram-negative, rod-shaped, motile, non-sporulating and non-fermenting bacteria were shown to belong to the genus Cupriavidus of the Betaproteobacteria on the basis of their 16S rRNA gene sequence analyses...
October 11, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29016326/human-intestinal-parasite-burden-and-poor-sanitation-in-rural-alabama
#15
Megan L McKenna, Shannon McAtee, Patricia E Bryan, Rebecca Jeun, Tabitha Ward, Jacob Kraus, Maria E Bottazzi, Peter J Hotez, Catherine C Flowers, Rojelio Mejia
Hookworm infection affects 430 million people worldwide, causing iron deficiency, impaired cognitive development, and stunting in children. Because of the environmental conditions needed for the hookworm life-cycle, this parasite is endemic to resource-limited countries. Necator americanus was endemic in the southern United States before improvement of sewage disposal systems and eradication programs. With continued poverty, poor sanitation, and an environment suitable for the hookworm life-cycle in some regions of the southern United States, a current prevalence study using modern molecular diagnostics is warranted...
November 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28988032/expanding-the-genetic-tool-box-for-cupriavidus-necator-by-a-stabilized-l-rhamnose-inducible-plasmid-system
#16
Anne Sydow, Andreas Pannek, Thomas Krieg, Ina Huth, Stéphane E Guillouet, Dirk Holtmann
The Gram negative bacterium Cupriavidus necator is well known for the accumulation of poly(3-hydroxybutyrate) and its fast lithoautotrophic growth, leading in high cell densities. Although the host was engineered for the heterologous production of diverse chemicals and biopolymers in recent years, tool box of stabilized inducible expression systems is still limited. To avoid plasmid loss during fermentation processes and to allow expression of complex proteins, a tunable L-rhamnose inducible system was established and characterized using enhanced green fluorescent protein (eGFP)...
October 4, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28987366/the-impact-of-gallic-acid-on-the-methotrexate-induced-kidney-damage-in-rats
#17
Halil Asci, Ozlem Ozmen, Hamit Yasar Ellidag, Bunyamin Aydin, Ercan Bas, Necat Yilmaz
Prolonged use of an antineoplastic agent methotrexate (MTX), can cause numerous side effects such as nephrotoxicity. The aim of this study was to examine the effects of MTX on kidneys and demonstrate the protective effects of gallic acid (GA). Twenty-four, male, rats distributed into three groups. Each groups consisted eight rats and only saline was administered to the control group. The MTX group received a single dose (20 mg/kg) MTX intraperitoneally. The MTX + GA group received same dose MTX and 100 mg/kg GA orally during the 7 days...
October 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28968014/-profiles-of-intestinal-polyparasitism-in-a-community-of-the-colombian-amazon-region
#18
Julián A Fernández-Niño, Claudia I Astudillo-García, Laura María Segura, Natalia Gómez, Ángela Skantria Salazar, Juan Hember Tabares, Cristian Andrés Restrepo, Miguel Ángel Ruiz, Myriam Consuelo López, Patricia Reyes
INTRODUCTION: Intestinal parasite infections and polyparasitism are highly prevalent in the most vulnerable populations of developing countries due to environmental, biological and social determinants common in the transmission of parasites. Children between 1 and 15 years of age are the most affected population. OBJECTIVE: To describe the prevalence and profiles of intestinal polyparasitism in 1 to 15 year-old children from native communities in the Colombian Amazon region...
September 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28955607/improved-fed-batch-production-of-high-purity-phb-poly-3-hydroxy-butyrate-by-cupriavidus-necator-mtcc-1472-from-sucrose-based-cheap-substrates-under-response-surface-optimized-conditions
#19
Pinaki Dey, Vivek Rangarajan
Experimental investigations were carried out for Cupriavidus necator (MTCC 1472)-based improved production of poly-3 hydroxy butyrate (PHB) through induced nitrogen limiting fed-batch cultivation strategies. Initially Plackett-Burman design and response surface methodology were implemented to optimize most influencing process parameters. With optimized process parameter values, continuous feeding strategies ware applied in a 5-l fermenter with table sugar concentration of 100 g/l, nitrogen concentration of 0...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28952552/the-molecular-level-characterization-of-biodegradable-polymers-originated-from-polyethylene-using-non-oxygenated-polyethylene-wax-as-a-carbon-source-for-polyhydroxyalkanoate-production
#20
Brian Johnston, Guozhan Jiang, David Hill, Grazyna Adamus, Iwona Kwiecień, Magdalena Zięba, Wanda Sikorska, Matthew Green, Marek Kowalczuk, Iza Radecka
There is an increasing demand for bio-based polymers that are developed from recycled materials. The production of biodegradable polymers can include bio-technological (utilizing microorganisms or enzymes) or chemical synthesis procedures. This report demonstrates the corroboration of the molecular structure of polyhydroxyalkanoates (PHAs) obtained by the conversion of waste polyethylene (PE) via non-oxygenated PE wax (N-PEW) as an additional carbon source for a bacterial species. The N-PEW, obtained from a PE pyrolysis reaction, has been found to be a beneficial carbon source for PHA production with Cupriavidus necator H16...
August 28, 2017: Bioengineering
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