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https://www.readbyqxmd.com/read/28632749/using-a-health-rating-system-to-evaluate-the-usefulness-of-caenorhabditis-elegans-as-a-model-for-anthelmintic-study
#1
Kathryn J Weaver, Cassandra J May, Brian L Ellis
Soil-transmitted helminths (STHs) are intestinal parasitic nematodes that infect humans, and are transmitted through contaminated soil. These nematodes include the large roundworm (Ascaris lumbricoides), whipworm (Trichuris trichiura), and hookworm (Ancylostoma ceylanicum, Ancylostoma duodenale, and Necator americanus). Nearly 1.5 billion people (~24% of the population) worldwide are infected with at least one species of these parasites, burdening the poor, in particular, children and pregnant women. To combat these diseases, the WHO only recognizes four anthelmintic drugs, including the preferred drug, albendazole, for mass drug administration (MDA)...
2017: PloS One
https://www.readbyqxmd.com/read/28628835/low-frequency-sonic-waves-assisted-cloud-point-extraction-of-polyhydroxyalkanoate-from-cupriavidus-necator
#2
Sivananth Murugesan, Regupathi Iyyaswami
Low frequency sonic waves, less than 10kHz were introduced to assist cloud point extraction of polyhydroxyalkanoate from Cupriavidus necator present within the crude broth. Process parameters including surfactant system variables and sonication parameters were studied for their effect on extraction efficiency. Introduction of low frequency sonic waves assists in the dissolution of microbial cell wall by the surfactant micelles and release of cellular content, polyhydroxyalkanoate granules released were encapsulated by the micelle core which was confirmed by crotonic acid assay...
June 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28610937/hookworm-infections-among-migrant-workers-in-malaysia-molecular-identification-of-necator-americanus-and-ancylostoma-duodenale
#3
Norhidayu Sahimin, Yvonne Ai Lian Lim, Benacer Douadi, Mohd Khairul Nizam Mohd Khalid, John-James Wilson, Jerzy M Behnke, Siti Nursheena Mohd Zain
Ongoing urbanisation of the working population as well as cross-border migration of workers particularly into large cities has contributed to the development and growth of urban slums. These deprived areas are conducive for the transmission of intestinal pathogens including hookworm. The aim of this study was to determine both the prevalence and species identity of hookworm infections among the migrant worker community in Malaysia. A total of 388 faecal samples were collected from migrant workers between September 2014 and August 2015, representing workers from five employment sectors: construction, manufacturing, agriculture and plantations, food services and domestic services...
June 10, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28591561/over-expression-of-groesl-in-cupriavidus-necator-for-heterotrophic-and-autotrophic-isopropanol-production
#4
Jillian Marc, Estelle Grousseau, Eric Lombard, Anthony J Sinskey, Nathalie Gorret, Stéphane E Guillouet
We previously reported a metabolic engineering strategy to develop an isopropanol producing strain of Cupriavidus necator leading to production of 3.4gL(-1) isopropanol. In order to reach higher titers, isopropanol toxicity to the cells has to be considered. A toxic effect of isopropanol on the growth of C. necator has been indeed observed above a critical value of 15gL(-1). GroESL chaperones were first searched and identified in the genome of C. necator. Native groEL and groES genes from C. necator were over-expressed in a strain deleted for PHA synthesis...
June 4, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28588567/biofilm-vs-planktonic-lifestyle-consequences-for-pesticide-2-4-d-metabolism-by-cupriavidus-necator-jmp134
#5
Thomas Z Lerch, Claire Chenu, Marie F Dignac, Enrique Barriuso, André Mariotti
The development of bacterial biofilms in natural environments may alter important functions, such as pollutant bioremediation by modifying both the degraders' physiology and/or interactions within the matrix. The present study focuses on the influence of biofilm formation on the metabolism of a pesticide, 2,4-dichlorophenoxyacetic acid (2,4-D), by Cupriavidus necator JMP134. Pure cultures were established in a liquid medium with 2,4-D as a sole carbon source with or without sand grains for 10 days. Bacterial numbers and 2,4-D concentrations in solution were followed by spectrophotometry, the respiration rate by gas chromatography and the surface colonization by electron microscopy...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28585326/chicken-feather-hydrolysate-as-inexpensive-complex-nitrogen-source-for-pha-production-by-cupriavidus-necator-on-waste-frying-oils
#6
P Benesova, D Kucera, I Marova, S Obruca
The chicken feather hydrolysate (FH) has been tested as potential complex nitrogen source for the production of polyhydroxyalkanoates by Cupriavidus necator H16 when waste frying oil was used as a carbon source. The addition of FH into the mineral salt media with decreased inorganic nitrogen source concentration improved the yields of biomass and polyhydrohyalkanoates. The highest yields were achieved when 10 vol. % of FH prepared by microwave assisted alkaline hydrolysis of 60 g l(-1) feather was added. In this case the poly(3-hydroxybutyrate) (PHB) yields were improved by more than about 50% as compared with control cultivation...
June 5, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28577450/n-acetylcysteine-induced-vasodilatation-is-modulated-by-katp-channels-na-k-atpase-activity-and-intracellular-calcium-concentration-an-in-vitro-study
#7
Özden Vezir, Ülkü Çömelekoğlu, Nehir Sucu, Ali Erdinç Yalın, Şakir Necat Yılmaz, Serap Yalın, Fatma Söğüt, Selma Yaman, Kezban Kibar, Merih Akkapulu, Meryem İlkay Koç, Didem Seçer
BACKGROUND: In this study, we aimed to investigate the role of ATP-sensitive potassium (KATP) channel, Na(+)/K(+)-ATPase activity, and intracellular calcium levels on the vasodilatory effect of N-acetylcysteine (NAC) in thoracic aorta by using electrophysiological and molecular techniques. METHODS: Rat thoracic aorta ring preparations and cultured thoracic aorta cells were divided into four groups as control, 2mM NAC, 5mM NAC, and 10mM NAC. Thoracic aorta rings were isolated from rats for measurements of relaxation responses and Na(+)/K(+)-ATPase activity...
March 31, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28553300/run1-and-ren1-pyramiding-in-grapevine-vitis-vinifera-cv-crimson-seedless-displays-an-improved-defense-response-leading-to-enhanced-resistance-to-powdery-mildew-erysiphe-necator
#8
Mario Agurto, Rudolf O Schlechter, Grace Armijo, Esteban Solano, Carolina Serrano, Rodrigo A Contreras, Gustavo E Zúñiga, Patricio Arce-Johnson
Fungal pathogens are the cause of the most common diseases in grapevine and among them powdery mildew represents a major focus for disease management. Different strategies for introgression of resistance in grapevine are currently undertaken in breeding programs. For example, introgression of several resistance genes (R) from different sources for making it more durable and also strengthening the plant defense response. Taking this into account, we cross-pollinated P09-105/34, a grapevine plant carrying both RUN1 and REN1 pyramided loci of resistance to Erysiphe necator inherited from a pseudo-backcrossing scheme with Muscadinia rotundifolia and Vitis vinifera 'Dzhandzhal Kara,' respectively, with the susceptible commercial table grape cv...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28542260/hookworm-infection-is-associated-with-decreased-cd4-t-cell-counts-in-hiv-infected-adult-ugandans
#9
Bozena M Morawski, Miya Yunus, Emmanuel Kerukadho, Grace Turyasingura, Logose Barbra, Andrew Mijumbi Ojok, Andrew R DiNardo, Stefanie Sowinski, David R Boulware, Rojelio Mejia
Most studies evaluating epidemiologic relationships between helminths and HIV have been conducted in the pre-ART era, and evidence of the impact of helminth infections on HIV disease progression remains conflicting. Less is known about helminth infection and clinical outcomes in HIV-infected adults receiving antiretroviral therapy (ART). We sampled HIV-infected adults for eight gastrointestinal parasites and correlated parasitic infection with demographic predictors, and clinical and immunologic outcomes. Contrasting with previous studies, we measured parasitic infection with a quantitative, highly sensitive and specific polymerase chain reaction (PCR) method...
May 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28509826/sclerostin-antibody-treatment-enhances-rotator-cuff-tendon-to-bone-healing-in-an-animal-model
#10
Shivam A Shah, Ioannis Kormpakis, Necat Havlioglu, Michael S Ominsky, Leesa M Galatz, Stavros Thomopoulos
BACKGROUND: Rotator cuff tears are a common source of pain and disability, and poor healing after repair leads to high retear rates. Bone loss in the humeral head before and after repair has been associated with poor healing. The purpose of the current study was to mitigate bone loss near the repaired cuff and improve healing outcomes. METHODS: Sclerostin antibody (Scl-Ab) treatment, previously shown to increase bone formation and strength in the setting of osteoporosis, was used in the current study to address bone loss and enhance rotator cuff healing in an animal model...
May 17, 2017: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28496205/characterisation-of-a-3-hydroxypropionic-acid-inducible-system-from-pseudomonas-putida-for-orthogonal-gene-expression-control-in-escherichia-coli-and-cupriavidus-necator
#11
Erik K R Hanko, Nigel P Minton, Naglis Malys
3-hydroxypropionic acid (3-HP) is an important platform chemical used as a precursor for production of added-value compounds such as acrylic acid. Metabolically engineered yeast, Escherichia coli, cyanobacteria and other microorganisms have been developed for the biosynthesis of 3-HP. Attempts to overproduce this compound in recombinant Pseudomonas denitrificans revealed that 3-HP is consumed by this microorganism using the catabolic enzymes encoded by genes hpdH, hbdH and mmsA. 3-HP-inducible systems controlling the expression of these genes have been predicted in proteobacteria and actinobacteria...
May 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28495518/prenatal-exposure-to-low-dose-diclofenac-sodium-does-not-affect-total-neuron-numbers-in-spinal-segment-t13-in-rats
#12
Murat Çetin Ragbetli, Mikail Kara, Neşe Çölçimen, Necat Koyun, Gamze Çakmak, Veysel Akyol, Omur Gulsum Deniz, Kıymet Kübra Yurt
The main purpose of the present study was to investigate the effects of diclofenac sodium (DS) on the total number of neurons in segment T13 of the spinal cord of offspring of pregnant rats using stereological methods. Eighteen adult female Wistar albino rats weighing 150-200g were used. Pregnant female rats were divided into three groups; a control group, a sham group and a DS (1mg/kg, intramuscular) exposed group. The DS and sham groups received injection from the 5(th) day of gestation to the 19(th). 28 days after birth, the offspring rats were perfused with 4% buffered formalin...
May 8, 2017: Journal of Chemical Neuroanatomy
https://www.readbyqxmd.com/read/28489889/an-environmental-assessment-and-risk-map-of-ascaris-lumbricoides-and-necator-americanus-distributions-in-manufahi-district-timor-leste
#13
Rebecca Wardell, Archie C A Clements, Aparna Lal, David Summers, Stacey Llewellyn, Suzy J Campbell, James McCarthy, Darren J Gray, Susana V Nery
BACKGROUND: In Timor-Leste there have been intermittent and ineffective soil-transmitted helminth (STH) deworming programs since 2004. In a resource-constrained setting, having information on the geographic distribution of STH can aid in prioritising high risk communities for intervention. This study aimed to quantify the environmental risk factors for STH infection and to produce a risk map of STH in Manufahi district, Timor-Leste. METHODOLOGY/PRINCIPAL FINDINGS: Georeferenced cross-sectional data and stool samples were obtained from 2,194 participants in 606 households in 24 villages in the Manufahi District as part of cross sectional surveys done in the context of the "WASH for Worms" randomised controlled trial...
May 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28476592/biosynthesis-of-p-3hb-co-3hhx-with-improved-molecular-weights-from-a-mixture-of-palm-olein-and-fructose-by-cupriavidus-necator-re2058-pcb113
#14
Paramasivam Murugan, Chee-Yuen Gan, Kumar Sudesh
A combination of palm olein (POl) and fructose was used as carbon source for the biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] by recombinant Cupriavidus necator Re2058/pCB113. Cultures grown using 5g/L PO alone as carbon source produced cell dry weight (CDW) of 5.13g/L, 67% PHA/CDW and accumulated a copolymer containing 27mol% 3HHx in shake flask cultures. When cultures were grown in 5g/L fructose alone as the carbon source they produced CDW of 2.32g/L, 11% PHA/CDW and accumulated only poly(3-hydroxybutyrate) [P(3HB)] homopolymer...
May 2, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28470373/expression-patterns-and-promoter-characteristics-of-the-vitis-quinquangularis-vqsts36-gene-involved-in-abiotic-and-biotic-stress-response
#15
Xiangjing Yin, Li Huang, Xiuming Zhang, Chunlei Guo, Hao Wang, Zhi Li, Xiping Wang
Resveratrol is a stilbene compound that is synthesized by plants in response to biotic stress and has been linked to health benefits associated with the consumption of certain foods and food products, such as grapes and wine. The final step in the biosynthesis of resveratrol is catalyzed by the enzyme stilbene synthase (STS). Here, we assessed the expression of two STS genes (VqSTS36 and VpSTS36) from the wild grape species Vitis quinquangularis (accession 'Shang-24'; powdery mildew (PM) resistant) and Vitis pseudoreticulata (accession 'Hunan-1'; PM susceptible) following infection by Uncinula necator (Schw...
May 4, 2017: Protoplasma
https://www.readbyqxmd.com/read/28456470/simple-and-rapid-method-for-isolation-and-quantitation-of-polyhydroxyalkanoate-by-sds-sonication-treatment
#16
Hisashi Arikawa, Shunsuke Sato, Tetsuya Fujiki, Keiji Matsumoto
We developed a new method for isolation and quantitation of polyhydroxyalkanoate (PHA) from culture broth. In this method, the cells were sonicated in sodium dodecyl sulfate (SDS) solution and centrifuged to recover PHA. The recovered PHA was rinsed with deionized water and ethanol, and then weighed after drying. Hazardous chemicals such as chloroform, methanol, and sulfuric acid were not used, and no expensive analytical instruments were needed. We applied this method to Cupriavidus necator culture broths that included various amounts of poly(3-hydroxybutyrate) (PHB) or poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) from flasks and jar fermentors...
April 26, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28446233/no-impact-of-strongylid-infections-on-the-detection-of-plasmodium-spp-in-faeces-of-western-lowland-gorillas-and-eastern-chimpanzees
#17
Mwanahamisi I Mapua, Barbora Pafčo, Jade Burgunder, Ilona Profousová-Pšenková, Angelique Todd, Chie Hashimoto, Moneeb A Qablan, David Modrý, Klára J Petrželková
BACKGROUND: Although a high genetic diversity of Plasmodium spp. circulating in great apes has been revealed recently due to non-invasive methods enabling detection in faecal samples, little is known about the actual mechanisms underlying the presence of Plasmodium DNA in faeces. Great apes are commonly infected by strongylid nematodes, including hookworms, which cause intestinal bleeding. The impact of strongylid infections on the detection of Plasmodium DNA in faeces was assessed in wild, western, lowland gorillas from Dzanga Sangha Protected Areas, Central African Republic and eastern chimpanzees from Kalinzu Forest Reserve, Uganda...
April 26, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28434228/rapid-biodegradation-of-the-herbicide-2-4-dichlorophenoxyacetic-acid-by-cupriavidus-gilardii-t-1
#18
Xiangwei Wu, Wenbo Wang, Junwei Liu, Dandan Pan, Xiaohui Tu, Pei Lv, Yi Wang, Haiqun Cao, Yawen Wang, Rimao Hua
Phytotoxicity and environmental pollution of residual herbicides have caused much public concern during the past several decades. An indigenous bacterial strain capable of degrading 2,4-dichlorophenoxyacetic acid (2,4-D), designated T-1, was isolated from soybean field soil and identified as Cupriavidus gilardii. Strain T-1 degraded 2,4-D 3.39 times more rapidly than the model strain Cupriavidus necator JMP134. T-1 could also efficiently degrade 2-methyl-4-chlorophenoxyacetic acid (MCPA), MCPA isooctyl ester, and 2-(2,4-dichlorophenoxy)propionic acid (2,4-DP)...
May 10, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28401146/effects-of-bunch-rot-botrytis-cinerea-and-powdery-mildew-erysiphe-necator-fungal-diseases-on-wine-aroma
#19
Angela Lopez Pinar, Doris Rauhut, Ernst Ruehl, Andrea Buettner
This study aimed to characterize the effects of bunch rot and powdery mildew on the primary quality parameter of wine, the aroma. The influence of these fungal diseases was studied by comparative Aroma Extract Dilution Analyses (AEDA) and sensory tests. The effect of bunch rot was investigated on three grape varieties, namely White Riesling, Red Riesling and Gewürztraminer and that of powdery mildew on the hybrid Gm 8622-3; thereby, samples were selected that showed pronounced cases of infection to elaborate potential currently unknown effects...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28390393/regulatory-monocytes-in-helminth-infections-insights-from-the-modulation-during-human-hookworm-infection
#20
Lívia Silva Araújo Passos, Pedro Henrique Gazzinelli-Guimarães, Tiago Antônio de Oliveira Mendes, Ana Clara Gazzinelli Guimarães, Denise da Silveira Lemos, Natasha Delaqua Ricci, Ricardo Gonçalves, Daniella Castanheira Bartholomeu, Ricardo Toshio Fujiwara, Lilian Lacerda Bueno
BACKGROUND: While the macrophage polarization is well characterized in helminth infections, the natural heterogeneity of monocytes with multiple cell phenotypes might influence the outcome of neglected diseases, such hookworm infection. Here, we report the profile of monocytes in human hookworm infections as a model to study the regulatory subpopulation of monocytes in helminth infections. METHODS: Blood samples were collected from 19 Necator americanus-infected individuals and 13 healthy individuals...
April 8, 2017: BMC Infectious Diseases
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