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He Fu, Jun-Jie Zhang, Ying Xu, Hong-Jun Chao, Ning-Yi Zhou
The ortho-nitrophenol (ONP) utilizer Alcaligenes sp. strain NyZ215, meta-nitrophenol (MNP) utilizer Cupriavidus necator JMP134 and para-nitrophenol (PNP) utilizer Pseudomonas sp. strain WBC-3 were assembled as a consortium to degrade three nitrophenol isomers in sequential batch reactors. Pilot test were conducted in flasks to demonstrate that a mixture of three mononitrophenols at 0.5 mM each could be mineralized by this microbial consortium within 84 hours. Interestingly, neither ONP nor MNP was degraded until PNP was almost consumed by strain WBC-3...
November 19, 2016: Letters in Applied Microbiology
Alejandro Muñoz de Las Heras, Diogo J Portugal-Nunes, Nathasha Rizza, Anders G Sandström, Marie F Gorwa-Grauslund
BACKGROUND: Poly-3-D-hydroxybutyrate (PHB) that is a promising precursor for bioplastic with similar physical properties as polypropylene, is naturally produced by several bacterial species. The bacterial pathway is comprised of the three enzymes β-ketothiolase, acetoacetyl-CoA reductase (AAR) and PHB synthase, which all together convert acetyl-CoA into PHB. Heterologous expression of the pathway genes from Cupriavidus necator has enabled PHB production in the yeast Saccharomyces cerevisiae from glucose as well as from xylose, after introduction of the fungal xylose utilization pathway from Scheffersomyces stipitis including xylose reductase (XR) and xylitol dehydrogenase (XDH)...
November 18, 2016: Microbial Cell Factories
Cengiz Tavusbay, Emrah Alper, Melek Gökova, Erdinç Kamer, Haldun Kar, Kemal Atahan, Oğuzhan Özşay, Özlem Gür, Necat Cin, Emir Çapkınoğlu, Evren Durak
BACKGROUND: Endoscopic retrograde cholangiopancreatography (ERCP)-related perforation is an infrequent complication. It is associated with significant morbidity and mortality. The present study is an evaluation of experience with management and outcomes of ERCP-related perforations and a review of relevant literature. METHODS: Between January 2008 and January 2015, a total of 9383 ERCPs were performed in endoscopy unit. A total of 29 perforations (0.33%) were identified and retrospectively reviewed...
September 2016: Ulusal Travma Ve Acil Cerrahi Dergisi, Turkish Journal of Trauma & Emergency Surgery: TJTES
Kajan Srirangan, Xuejia Liu, Tam T Tran, Trevor C Charles, Murray Moo-Young, C Perry Chou
While poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] is a biodegradable commodity plastic with broad applications, its microbial synthesis is hindered by high production costs primarily associated with the supplementation of related carbon substrates (e.g. propionate or valerate). Here we report construction of engineered Escherichia coli strains for direct synthesis of P(3HB-co-3HV) from an unrelated carbon source (e.g. glucose or glycerol). First, an E. coli strain with an activated sleeping beauty mutase (Sbm) operon was used to generate propionyl-CoA as a precursor...
November 7, 2016: Scientific Reports
Gültekin Övet, Necat Alataş, Mehmet Şentürk, Fatih Güzelkara
OBJECTIVE: It is an ongoing debate whether tympanoplasty should be performed in the pediatric age group. The purpose of this study was to analyze and compare the anatomical and functional outcomes of type I cartilage tympanoplasty in children and adults. MATERIALS AND METHODS: The file records of patients who underwent type 1 cartilage tympanoplasty were retrospectively reviewed. In total, 133 patients who underwent type 1 tympanoplasty for chronic otitis media were included...
August 1, 2016: Journal of International Advanced Otology
Ota Samek, Stanislav Obruča, Martin Šiler, Petr Sedláček, Pavla Benešová, Dan Kučera, Ivana Márova, Jan Ježek, Silva Bernatová, Pavel Zemánek
We report herein on the application of Raman spectroscopy to the rapid quantitative analysis of polyhydroxyalkanoates (PHAs), biodegradable polyesters accumulated by various bacteria. This theme was exemplified for quantitative detection of the most common member of PHAs, poly(3-hydroxybutyrate) (PHB) in Cupriavidus necator H16. We have identified the relevant spectral region (800-1800 cm(-1)) incorporating the Raman emission lines exploited for the calibration of PHB (PHB line at 1736 cm(-1)) and for the selection of the two internal standards (DNA at 786 cm(-1) and Amide I at 1662 cm(-1))...
October 28, 2016: Sensors
Hisashi Arikawa, Keiji Matsumoto
BACKGROUND: Cupriavidus necator has attracted much attention as a platform for the production of polyhydroxyalkanoate (PHA) and other useful materials. Therefore, an appropriate modulation of gene expression is needed for producing the desired materials effectively. However, there is insufficient information on the genetic engineering techniques required for this in C. necator. RESULTS: We found that the disruption of a potential ribosome binding site (RBS) in the phaC1 gene in C...
October 28, 2016: Microbial Cell Factories
Hülya Ayık Aydın, Aysel Derbent Uysal, Onur Erol, Hamit Yaşar Ellidağ, Aslı Bayındır, Necat Yılmaz
We aimed to determine the relationship between serum ghrelin levels and large-for-gestational-age (LGA) fetuses in patients with gestational diabetes mellitus (GDM). A case-control study was conducted in 32 women with GDM and LGA fetuses (GDM + LGA group), 35 women with GDM and appropriate-for-gestational-age (AGA) fetuses (GDM + AGA group), 32 women with normal glucose tolerance (NGT) and LGA fetuses (NGT + LGA group), and 31 women with NGT and AGA fetuses (NGT + AGA group). All participants were recruited at the time of GDM diagnosis between 24 and 30 weeks of pregnancy...
October 28, 2016: Gynecological Endocrinology
Siti Baidurah, Paramasivam Murugan, Lee Joyyi, Junya Fukuda, Masaya Yamada, Kumar Sudesh, Yasuyuki Ishida
Thermally assisted hydrolysis and methylation-gas chromatography (THM-GC) in the presence of an organic alkali was validated for the compositional analysis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] accumulated in whole bacterial cells. Recombinant Cupriavidus necator Re2058/pCB113 was grown in a batch fermentation with different concentration of palm oil and fructose in order to control the molar fraction of 3HHx in P(3HB-co-3HHx) produced in the cells. Trace amounts (30μg) of freeze-dried cells were directly subjected to THM-GC in the presence of tetramethylammonium hydroxide (TMAH) at 400°C...
October 14, 2016: Journal of Chromatography. A
Paramasivam Murugan, Lizhu Han, Chee-Yuen Gan, Frans H J Maurer, Kumar Sudesh
Bacterial polyhydroxyalkanoates (PHA) are expensive partly due to the recovery and purification processes. Thus, many studies have been carried out in order to minimize the cost. Here we report on the use of mealworm, which is the larva of mealworm beetle (Tenebrio molitor) to recover PHA granules from Cupriavidus necator. Mealworms were shown to readily consume the freeze-dried C. necator cells and excrete the PHA granules in the form of whitish feces. Further purification using water, detergent and heat resulted in almost 100% pure PHA granules...
October 13, 2016: Journal of Biotechnology
Yoong Kit Leong, John Chi-Wei Lan, Hwei-San Loh, Tau Chuan Ling, Chien Wei Ooi, Pau Loke Show
Polyhydroxyalkanoates (PHAs), a class of renewable and biodegradable green polymers, have gained attraction as a potential substitute for the conventional plastics due to the increasing concern towards environmental pollution as well as the rapidly depleting petroleum reserve. Nevertheless, the high cost of downstream processing of PHA has been a bottleneck for the wide adoption of PHAs. Among the options of PHAs recovery techniques, aqueous two-phase extraction (ATPE) outshines the others by having the advantages of providing a mild environment for bioseparation, being green and non-toxic, the capability to handle a large operating volume and easily scaled-up...
October 10, 2016: Journal of Bioscience and Bioengineering
Elizabeth C Wittenborn, Marco Jost, Yifeng Wei, JoAnne Stubbe, Catherine L Drennan
Polyhydroxybutyrate synthase (PhaC) catalyzes the polymerization of 3-(R)-hydroxybutyryl-coenzyme A as a means of carbon storage in many bacteria. The resulting polymers can be used to make biodegradable materials with properties similar to those of thermoplastics and are an environmentally friendly alternative to traditional petroleum-based plastics. A full biochemical and mechanistic understanding of this process has been hindered in part by a lack of structural information on PhaC. Here we present the first structure of the catalytic domain (residues 201-589) of the class I PhaC from Cupriavidus necator (formerly Ralstonia eutropha) to 1...
November 25, 2016: Journal of Biological Chemistry
Hamit Yasar Ellidag, Necat Yilmaz, Fatma Kurtulus, Ozgur Aydin, Esin Eren, Ayca Inci, Suleyman Dolu, Fatma Demet Arslan Ince, Özlem Giray, Aylin Yaman
BACKGROUND: The klotho (Klt)-fibroblast growth factor-23 (FGF-23)-vitamin D axis is the main component of calcium (Ca) and phosphorus (P) metabolisms; on the contrary, it is also secreted from the choroid plexus (CP). PURPOSE: This study is aimed at evaluating serum soluble Klt (sKlt), FGF-23, and 25-(OH)-vitamin D levels in multiple sclerosis (MS) patients. METHODS: Thirty-two relapsing-remitting MS patients (11 males and 21 females; mean age 38...
September 2016: Annals of Neurosciences
R G Yousuf, J B Winterburn
Poly-3-hydroxybutrate (PHB) is a biodegradable polymer synthesised via bacterial fermentation as a means of storing carbon and energy under unbalanced growth conditions. The production cost of petroleum-based plastics is currently lower than that for biopolymers, and the carbon source is the most significant contributor to biopolymer production cost. A feasibility study to assess the suitability of using a date seed derived media as an alternative for PHB production under various stress conditions was investigated...
September 29, 2016: Bioresource Technology
Denis Vadlja, Martin Koller, Mario Novak, Gerhart Braunegg, Predrag Horvat
Statistical distribution of cell and poly[3-(R)-hydroxybutyrate] (PHB) granule size and number of granules per cell are investigated for PHB production in a five-stage cascade (5CSTR). Electron microscopic pictures of cells from individual cascade stages (R1-R5) were converted to binary pictures to visualize footprint areas for polyhydroxyalkanoate (PHA) and non-PHA biomass. Results for each stage were correlated to the corresponding experimentally determined kinetics (specific growth rate μ and specific productivity π)...
December 2016: Applied Microbiology and Biotechnology
Hua Shen, Ioannis Kormpakis, Necat Havlioglu, Stephen W Linderman, Shelly E Sakiyama-Elbert, Isaac E Erickson, Thomas Zarembinski, Matthew J Silva, Richard H Gelberman, Stavros Thomopoulos
BACKGROUND: The clinical outcomes following intrasynovial flexor tendon repair are highly variable. Excessive inflammation is a principal factor underlying the formation of adhesions at the repair surface and affecting matrix regeneration at the repair center that limit tendon excursion and impair tendon healing. A previous in-vitro study revealed that adipose-derived mesenchymal stromal cells (ASCs) modulate tendon fibroblast response to macrophage-induced inflammation. The goal of the current study was therefore to explore the effectiveness of autologous ASCs on the inflammatory stage of intrasynovial tendon healing in vivo using a clinically relevant animal model...
September 27, 2016: Stem Cell Research & Therapy
Junfei Wei, Ashish Damania, Xin Gao, Zhuyun Liu, Rojelio Mejia, Makedonka Mitreva, Ulrich Strych, Maria Elena Bottazzi, Peter J Hotez, Bin Zhan
BACKGROUND: The intestine of hookworms contains enzymes and proteins involved in the blood-feeding process of the parasite and is therefore a promising source of possible vaccine antigens. One such antigen, the hemoglobin-digesting intestinal aspartic protease known as Na-APR-1 from the human hookworm Necator americanus, is currently a lead candidate antigen in clinical trials, as is Na-GST-1 a heme-detoxifying glutathione S-transferase. METHODS: In order to discover additional hookworm vaccine antigens, messenger RNA was obtained from the intestine of male hookworms, Ancylostoma ceylanicum, maintained in hamsters...
September 27, 2016: Parasites & Vectors
Ivana Seccareccia, Ákos T Kovács, Ramses Gallegos-Monterrosa, Markus Nett
Cupriavidus necator is a non-obligate bacterial predator of Gram-negative and Gram-positive bacteria. In this study, we set out to determine the conditions, which are necessary to observe predatory behavior of C. necator. Using Bacillus subtilis as a prey organism, we confirmed that the predatory performance of C. necator is correlated with the available copper level, and that the killing is mediated, at least in part, by secreted extracellular factors. The predatory activity depends on the nutrition status of C...
November 2016: Microbiological Research
Bui Khac Hung, Nguyen Van De, Le Van Duyet, Jong-Yil Chai
To know the infection status of helminths in primary schoolchildren of southern parts of Vietnam, we performed an epidemiological study in Krong Pac district, Dak Lak Province, Vietnam. A total of 1,206 stool specimens were collected from ethnic Ede schoolchildren in 4 primary schools in 2015 and examined by the Kato-Katz technique. In addition, stool cultures were done by the Harada-Mori method to obtain hookworm larvae and then to clarify the species of hookworms infected. The results showed that the helminth infection rate was 25...
August 2016: Korean Journal of Parasitology
Subhamay Panda, Santamay Panda, Leena Kumari
Parasitic worm infection of humans is one of the most commonly prevalent helminth infection that has imposed great impact on society and public health in the developing world. The two species of hookworm, namely Ancylostoma duodenale and Necator americanus may be primarily responsible for causing parasitic infections in human beings. The highly prevalent areas for Ancylostoma duodenale infections are mainly India, Middle East, Australia, northern Africa and other parts of the world. The serum arylesterases/paraoxonases are family of enzymes that is involved in the hydrolysis of a number of organophosphorus insecticides to the nontoxic products...
July 2016: International Journal of Pharma and Bio Sciences
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