Júlia Solana-Balaguer, Núria Martín-Flores, Pol Garcia-Segura, Genís Campoy-Campos, Leticia Pérez-Sisqués, Almudena Chicote-González, Joaquín Fernández-Irigoyen, Enrique Santamaría, Esther Pérez-Navarro, Jordi Alberch, Cristina Malagelada
Extracellular vesicles (EVs) play a crucial role in intercellular communication, participating in the paracrine trophic support or in the propagation of toxic molecules, including proteins. RTP801 is a stress-regulated protein, whose levels are elevated during neurodegeneration and induce neuron death. However, whether RTP801 toxicity is transferred trans-neuronally via EVs remains unknown. Hence, we overexpressed or silenced RTP801 protein in cultured cortical neurons, isolated their derived EVs (RTP801-EVs or shRTP801-EVs, respectively), and characterized EVs protein content by mass spectrometry (MS)...
November 2023: Journal of Extracellular Vesicles