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https://www.readbyqxmd.com/read/28584920/co-culture-of-dedifferentiated-and-primary-human-chondrocytes-obtained-from-cadaveric-donor-enhance-the-histological-quality-of-repair-tissue-an-in-vivo-animal-study
#1
Anell Olivos-Meza, Cristina Velasquillo Martínez, Brenda Olivos Díaz, Carlos Landa-Solís, Mats Brittberg, Raul Pichardo Bahena, Carmina Ortega Sanchez, Valentin Martínez, Enrique Alvarez Lara, José Clemente Ibarra-Ponce de León
To compare the quality of the repair tissue in three-dimensional co-culture of human chondrocytes implanted in an in vivo model. Six cadaveric and five live human donors were included. Osteochondral biopsies from the donor knees were harvested for chondrocyte isolation. Fifty percent of cadaveric chondrocytes were expanded until passage-2 (P2) while the remaining cells were cryopreserved in passage-0 (P0). Fresh primary chondrocytes (P0f) obtained from live human donors were co-cultured. Three-dimensional constructs were prepared with a monolayer of passage-2 chondrocytes, collagen membrane (Geistlich Bio-Gide(®)), and pellet of non-co-cultured (P2) or co-cultured chondrocytes (P2 + P0c, P2 + P0f)...
June 5, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28582970/a-novel-constructed-spt15-mutagenesis-library-of-saccharomyces-cerevisiae-by-using-gtme-technique-for-enhanced-ethanol-production
#2
Ashraf A M M El-Rotail, Liang Zhang, Youran Li, Shuang Ping Liu, Gui Yang Shi
During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the Saccharomyces cerevisiae genome via screening the gTME approach in order to obtain a new mutant S. cerevisiae diploid strain. The vector pYX212 was utilized to transform these genes into the control diploid strain S...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28578293/bio-inspired-crosslinking-and-matrix-drug-interactions-for-advanced-wound-dressings-with-long-term-antimicrobial-activity
#3
Chetna Dhand, Mayandi Venkatesh, Veluchami Amutha Barathi, Sriram Harini, Samiran Bairagi, Eunice Goh Tze Leng, Nandhakumar Muruganandham, Kenny Zhi Wei Low, Mobashar Hussain Urf Turabe Fazil, Xian Jun Loh, Dinesh Kumar Srinivasan, Shou Ping Liu, Roger W Beuerman, Navin Kumar Verma, Seeram Ramakrishna, Rajamani Lakshminarayanan
There is a growing demand for durable advanced wound dressings for the management of persistent infections after deep burn injuries. Herein, we demonstrated the preparation of durable antimicrobial nanofiber mats, by taking advantage of strong interfacial interactions between polyhydroxy antibiotics (with varying number of OH groups) and gelatin and their in-situ crosslinking with polydopamine (pDA) using ammonium carbonate diffusion method. Polydopamine crosslinking did not interfere with the antimicrobial efficacy of the loaded antibiotics...
May 26, 2017: Biomaterials
https://www.readbyqxmd.com/read/28464773/the-role-of-atypical-antipsychotics-in-sexuality-road-to-recovery-in-schizophrenia
#4
Raja Lope Adam, Hatta Sidi, Marhani Midin, Hazli Zakaria, Srijit Das, Khairi Che Mat
Sexuality is an important dimension in human beings as a form of expression of individuality. For many decades, sexual functioning has been a neglected area among patients suffering from schizophrenia. It was a presumption that patients with schizophrenia could be asexual and this could be secondary to overwhelming situations of delusion, hallucination, hostility and negative symptoms among others. The deficient in sexual functioning are due to innate factors, i.e. negative symptoms (apathy, avolition and amotivation) and also as a result of prefrontal dysfunction, i...
May 2, 2017: Current Drug Targets
https://www.readbyqxmd.com/read/27995589/comparative-of-fibroblast-and-osteoblast-cells-adhesion-on-surface-modified-nanofibrous-substrates-based-on-polycaprolactone
#5
Fereshteh Sharifi, Shiva Irani, Mojgan Zandi, Masoud Soleimani, Seyed Mohammad Atyabi
One of the determinant factors for successful bioengineering is to achieve appropriate nano-topography and three-dimensional substrate. In this research, polycaprolactone (PCL) nano-fibrous mat with different roughness modified with O2 plasma was fabricated via electrospinning. The purpose of this study was to evaluate the effect of plasma modification along with surface nano-topography of mats on the quality of human fibroblast (HDFs) and osteoblast cells (OSTs)-substrate interaction. Surface properties were studied using scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle, Fourier-transformation infrared spectroscopy...
December 2016: Progress in biomaterials
https://www.readbyqxmd.com/read/27847250/recyclable-saccharomyces-cerevisiae-loaded-nanofibrous-mats-with-sandwich-structure-constructing-via-bio-electrospraying-for-heavy-metal-removal
#6
Shangjing Xin, Zhaoyang Zeng, Xue Zhou, Wenjing Luo, Xiaowen Shi, Qun Wang, Hongbing Deng, Yumin Du
Biosorbents, such as algae and yeast, have been applied in heavy metal adsorption due to their low cost and efficacy. However, they cannot be recycled and reused after direct application, which may cause a secondary pollution. In this study, we used bio-electrospraying technique to immobilize Saccharomyces cerevisiae (a byproduct from food fermentation) onto the surface of poly(ε-caprolactone)/chitosan/rectorite ternary composites based nanofibrous mats. This technique not only combined the advantages of both S...
February 15, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27779795/dna-extraction-from-benthic-cyanobacteria-comparative-assessment-and-optimization
#7
COMPARATIVE STUDY
V Gaget, A Keulen, M Lau, P Monis, J D Brookes
AIMS: Benthic Cyanobacteria produce toxic and odorous compounds similar to their planktonic counterparts, challenging the quality of drinking water supplies. The biofilm that benthic algae and other micro-organisms produce is a complex and protective matrix. Monitoring to determine the abundance and identification of Cyanobacteria, therefore, relies on molecular techniques, with the choice of DNA isolation technique critical. This study investigated which DNA extraction method is optimal for DNA recovery in order to guarantee the best DNA yield for PCR-based analysis of benthic Cyanobacteria...
January 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27475728/bio-inspired-in-situ-crosslinking-and-mineralization-of-electrospun-collagen-scaffolds-for-bone-tissue-engineering
#8
Chetna Dhand, Seow Theng Ong, Neeraj Dwivedi, Silvia Marrero Diaz, Jayarama Reddy Venugopal, Balchandar Navaneethan, Mobashar H U T Fazil, Shouping Liu, Vera Seitz, Erich Wintermantel, Roger W Beuerman, Seeram Ramakrishna, Navin K Verma, Rajamani Lakshminarayanan
Bone disorders are the most common cause of severe long term pain and physical disability, and affect millions of people around the world. In the present study, we report bio-inspired preparation of bone-like composite structures by electrospinning of collagen containing catecholamines and Ca(2+). The presence of divalent cation induces simultaneous partial oxidative polymerization of catecholamines and crosslinking of collagen nanofibers, thus producing mats that are mechanically robust and confer photoluminescence properties...
October 2016: Biomaterials
https://www.readbyqxmd.com/read/27443952/chemical-mimicking-of-bio-assisted-aluminium-extraction-by-aspergillus-niger-s-exometabolites
#9
Katarína Boriová, Martin Urík, Marek Bujdoš, Ivana Pifková, Peter Matúš
Presence of microorganisms in soils strongly affects mobility of metals. This fact is often excluded when mobile metal fraction in soil is studied using extraction procedures. Thus, the first objective of this paper was to evaluate strain Aspergillus niger's exometabolites contribution on aluminium mobilization. Fungal exudates collected in various time intervals during cultivation were analyzed and used for two-step bio-assisted extraction of alumina and gibbsite. Oxalic, citric and gluconic acids were identified in collected culture media with concentrations up to 68...
November 2016: Environmental Pollution
https://www.readbyqxmd.com/read/27338013/design-of-functionalized-biodegradable-pha-based-electrospun-scaffolds-meant-for-tissue-engineering-applications
#10
Daniel Grande, Julien Ramier, Davy Louis Versace, Estelle Renard, Valérie Langlois
Modification of electrospun nanofibrous poly(3-hydroxyalkanoate) (PHA)-based mats was implemented through two routes to obtain biomimetic scaffolds meant for tissue engineering applications. The first strategy relied on a physical functionalization of scaffolds thanks to an original route which combined both electrospinning and electrospraying, while the second approach implied the chemical modification of fiber surface via the introduction of reactive functional groups to further conjugate bioactive molecules...
July 25, 2017: New Biotechnology
https://www.readbyqxmd.com/read/27307116/international-charter-of-principles-for-sharing-bio-specimens-and-data
#11
Deborah Mascalzoni, Edward S Dove, Yaffa Rubinstein, Hugh Js Dawkins, Anna Kole, Pauline McCormack, Simon Woods, Olaf Riess, Franz Schaefer, Hanns Lochmüller, Bartha M Knoppers, Mats Hansson
No abstract text is available yet for this article.
July 2016: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/27185121/development-of-polyamide-6-6-chitosan-electrospun-hybrid-nanofibrous-scaffolds-for-tissue-engineering-application
#12
Bishnu Kumar Shrestha, Hamouda M Mousa, Arjun Prasad Tiwari, Sung Won Ko, Chan Hee Park, Cheol Sang Kim
The development of biofunctional and bioactive hybrid polymeric scaffolds seek to mitigate the current challenges in the emerging field of tissue engineering. In this paper, we report the fabrication of a biomimetic and biocompatible nanofibrous scaffolds of polyamide-6,6 (PA-6,6) blended with biopolymer chitosan via one step co-electrospinning technique. Different weight percentage of chitosan 10wt%, 15wt%, and 20wt% were blended with PA-6,6, respectively. The nanocomposite electrospun scaffolds mats enabled to provide the osteophilic environment for cells growth and biomineralization...
September 5, 2016: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27120656/removal-of-pharmaceutical-residues-using-ozonation-as-intermediate-process-step-at-link%C3%A3-ping-wwtp-sweden
#13
Christian Baresel, Jonas Malmborg, Mats Ek, Robert Sehlén
Pilot tests as basis for the design, implementation and operation of a future full-scale oxidation plant completing the existing sewage treatment in Linköping, Sweden, were performed. Using an ozonation step between bio-sedimentation and post-denitrification processes, the primary goal was the removal of the highest priority substances to effluent water levels that will not cause adverse effects in the recipient considering the natural dilution. The study included initial emission screenings, dose control trials, treatment performance studies and eco-toxicity studies...
2016: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/26706521/photocatalytic-self-cleaning-poly-l-lactide-materials-based-on-a-hybrid-between-nanosized-zinc-oxide-and-expanded-graphite-or-fullerene
#14
Daniela Virovska, Dilyana Paneva, Nevena Manolova, Iliya Rashkov, Daniela Karashanova
New self-cleaning materials of polymer fibers decorated with a hybrid between nanosized zinc oxide and expanded graphite (EG) or fullerene (C60) were obtained. The new materials were prepared by applying electrospinning in conjunction with electrospraying. Poly(l-lactide) (PLA) was selected as a biocompatible and (bio)degradable polymer carrier. PLA solution was electrospun in combination with electrospraying of a suspension that contained the ZnO/EG or ZnO/C60 hybrid. Mats with different content of EG or C60 were obtained...
March 2016: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/26614558/polydioxanone-based-bio-materials-for-tissue-engineering-and-drug-gene-delivery-applications
#15
REVIEW
Nowsheen Goonoo, Roubeena Jeetah, Archana Bhaw-Luximon, Dhanjay Jhurry
Since the commercialization of polydioxanone (PDX) as a biodegradable monofilament suture by Ethicon in 1981, the polymer has received only limited interest until recently. The limitations of polylactide-co-glycolide (PLGA) coupled with the growing need for materials with enhanced features and the advent of new fabrication techniques such as electrospinning have revived interest for PDX in medical devices, tissue engineering and drug delivery applications. Electrospun PDX mats show comparable mechanical properties as the major structural components of native vascular extracellular matrix (ECM) i...
November 2015: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/26478309/osteogenesis-of-human-adipose-derived-stem-cells-on-poly-dopamine-coated-electrospun-poly-lactic-acid-fiber-mats
#16
Chi-Chang Lin, Shu-Juan Fu
Electrospinning is a versatile technique to generate large quantities of micro- or nano-fibers from a wide variety of shapes and sizes of polymer. The aim of this study is to develop functionalized electrospun nano-fibers and use a mussel-inspired surface coating to regulate adhesion, proliferation and differentiation of human adipose-derived stem cells (hADSCs). We prepared poly(lactic acid) (PLA) fibers coated with polydopamine (PDA). The morphology, chemical composition, and surface properties of PDA/PLA were characterized by SEM and XPS...
January 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/26358740/sinus-floor-augmentation-using-straumann%C3%A2-boneceramic%C3%A2-and-bio-oss%C3%A2-in-a-split-mouth-design-and-later-placement-of-implants-a-5-year-report-from-a-longitudinal-study
#17
Arne Mordenfeld, Christer Lindgren, Mats Hallman
BACKGROUND: Straumann® BoneCeramic™ is a synthetic biphasic calcium phosphate (BCP) aimed for sinus floor augmentation. Long-term follow-up of implants placed in BCP after sinus augmentation is still missing. PURPOSE: The primary aim of the study was to compare survival rates and marginal bone loss of Straumann SLActive implants placed in either BCP (test) or Bio-Oss® (DBB) (control) after sinus floor augmentation. The secondary aim was to calculate graft sinus height at different time points...
October 2016: Clinical Implant Dentistry and related Research
https://www.readbyqxmd.com/read/26314367/fluorescence-recovery-after-photobleaching-in-material-and-life-sciences-putting-theory-into-practice
#18
Niklas Lorén, Joel Hagman, Jenny K Jonasson, Hendrik Deschout, Diana Bernin, Francesca Cella-Zanacchi, Alberto Diaspro, James G McNally, Marcel Ameloot, Nick Smisdom, Magnus Nydén, Anne-Marie Hermansson, Mats Rudemo, Kevin Braeckmans
Fluorescence recovery after photobleaching (FRAP) is a versatile tool for determining diffusion and interaction/binding properties in biological and material sciences. An understanding of the mechanisms controlling the diffusion requires a deep understanding of structure-interaction-diffusion relationships. In cell biology, for instance, this applies to the movement of proteins and lipids in the plasma membrane, cytoplasm and nucleus. In industrial applications related to pharmaceutics, foods, textiles, hygiene products and cosmetics, the diffusion of solutes and solvent molecules contributes strongly to the properties and functionality of the final product...
August 2015: Quarterly Reviews of Biophysics
https://www.readbyqxmd.com/read/25794811/optimization-and-characterization-of-bio-oil-produced-by-microwave-assisted-pyrolysis-of-oil-palm-shell-waste-biomass-with-microwave-absorber
#19
Faisal Mushtaq, Tuan Amran Tuan Abdullah, Ramli Mat, Farid Nasir Ani
In this study, solid oil palm shell (OPS) waste biomass was subjected to microwave pyrolysis conditions with uniformly distributed coconut activated carbon (CAC) microwave absorber. The effects of CAC loading (wt%), microwave power (W) and N2 flow rate (LPM) were investigated on heating profile, bio-oil yield and its composition. Response surface methodology based on central composite design was used to study the significance of process parameters on bio-oil yield. The coefficient of determination (R(2)) for the bio-oil yield is 0...
August 2015: Bioresource Technology
https://www.readbyqxmd.com/read/25299642/stoichiometry-of-root-and-leaf-nitrogen-and-phosphorus-in-a-dry-alpine-steppe-on-the-northern-tibetan-plateau
#20
Jiangtao Hong, Xiaodan Wang, Jianbo Wu
Leaf nitrogen (N) and phosphorus (P) have been used widely in the ecological stoichiometry to understand nutrient limitation in plant. However,few studies have focused on the relationship between root nutrients and environmental factors. The main objective of this study was to clarify the pattern of root and leaf N and P concentrations and the relationships between plant nitrogen (N) and phosphorus (P) concentrations with climatic factors under low temperature conditions in the northern Tibetan Plateau of China...
2014: PloS One
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