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https://www.readbyqxmd.com/read/29777495/ameliorative-effect-of-ginseng-extract-on-phthalate-and-bisphenol-a-reprotoxicity-during-pregnancy-in-rats
#1
Islam M Saadeldin, Mohamed A Hussein, Aida Hamid Suleiman, Mahmoud G Abohassan, Mona M Ahmed, Amr A Moustafa, Abdullah F Moumen, Ayman Abdel-Aziz Swelum
Phthalates (such as DEHP) and bisphenol A (BPA) are widely used chemicals in plastics manufacturing and exert public health concerns as endocrine disrupters. This study was designed to investigate the deleterious effect of DEHP and BPA on endocrine profile of pregnant female rats and the combined treatment with ginseng extract (Panax ginseng). Seventy-two pregnant rats were divided into six groups (control, ginseng, DEHP, BPA, Gin + DEHP, and Gin + BPA), 12 females per each group. The drugs were supplemented from pregnancy day 0 until day 20...
May 18, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29776928/enrichment-of-clinically-relevant-organisms-in-spontaneous-preterm-delivered-placenta-and-reagent-contamination-across-all-clinical-groups-in-a-large-uk-pregnancy-cohort
#2
Lydia J Leon, Ronan Doyle, Ernest Diez-Benavente, Taane G Clark, Nigel Klein, Philip Stanier, Gudrun E Moore
In this study differences in the placental microbiota of term and preterm deliveries from a large UK pregnancy cohort were studied using 16S targeted amplicon sequencing. The impact of contamination from DNA extraction, PCR reagents, as well as those from delivery itself were also examined. A total of 400 placental samples from 256 singleton pregnancies were analysed and differences investigated between spontaneous preterm, non-spontaneous preterm, and term delivered placenta. DNA from recently delivered placenta was extracted, and screening for bacterial DNA was carried out using targeted sequencing of the 16S rRNA gene on the Illumina MiSeq platform...
May 18, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29754504/hominis-placenta-suppresses-acute-lung-inflammation-by-activating-nrf2
#3
Tae Ho Kim, Jun-Yong Choi, Kyun Ha Kim, Min Jung Kwun, Chang-Woo Han, Ran Won, Jung Ju Lee, Jong-In Kim, Myungsoo Joo
Hominis placenta (HP), a dried human placenta, has been known to target liver, lung, or kidney meridians, improving the functions associated with these meridians in traditional Chinese or Asian medicine (TCM). Since recent studies implicate an HP extract in suppressing inflammation, we investigated whether an aqueous HP extract can ameliorate inflammation that occurred in the lungs. When administered with a single intratracheal lipopolysaccharide (LPS), C57BL/6 mice developed an acute neutrophilic lung inflammation along with an increased expression of pro-inflammatory cytokine genes...
May 13, 2018: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/29740884/bovine-foetal-sex-determination-different-dna-extraction-and-amplification-approaches-for-efficient-livestock-production
#4
M Ristanic, Lj Stanisic, M Maletic, U Glavinic, V Draskovic, N Aleksic, Z Stanimirovic
Foetal sex determination using polymerase chain reaction (PCR) in mammals is based on the amplification of gender-specific foetal DNA sequences circulating in maternal blood. The bovine synepitheliochorial placenta does not allow a direct contact between the trophoblast and the maternal blood, resulting in difficult passage of foetal DNA and, consequently, its very small amounts in maternal bloodstream. Circulating cell-free foetal DNA (ccffDNA) encompasses short nucleotide fragments (300-600 bp) in maternal circulation...
May 8, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29739458/anti-stress-effects-of-human-placenta-extract-possible-involvement-of-the-oxidative-stress-system-in-rats
#5
Hyun-Jung Park, Hyun Soo Shim, Sunyoung Lee, Dae Hyun Hahm, Hyejung Lee, Chang Taek Oh, Hae Jung Han, Hyi Jeong Ji, Insop Shim
BACKGROUND: Human placenta hydrolysate (hPH) has been utilized to improve menopausal, fatigue, liver function. Its high concentration of bioactive substances is known to produce including antioxidant, anti-inflammatory and anti-nociceptive activities. However, its mechanisms of stress-induced depression remain unknown. METHODS: The present study examined the effect of hPH on stress-induced depressive behaviors and biochemical parameters in rats. hPH (0.02 ml, 0...
May 8, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29737550/the-association-of-the-placental-caspase-3-gene-polymorphisms-and-preeclampsia-susceptibility-and-in-silico-analysis
#6
Batool Teimoori, Atefeh Yazdi, Mahnaz Rezaei, Abbas Mohammadpour-Gharehbagh, Danial Jahantigh, Saeedeh Salimi
Preeclampsia is a pathologic complication of pregnancy, associated with increased apoptosis in the cytotrophoblasts as the main cause of this disorder. Caspase-3 is a key apoptosis-related enzyme that both mitochondrial and death receptor apoptotic pathways can activate. In this study, we aimed to investigate the effect of placental CASP-3 rs4647602 and rs4647610 polymorphisms on PE susceptibility. The placentas of 106 PE women and 115 normotensive pregnant women were collected. Genomic DNA was extracted from the placenta...
May 8, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29718759/increased-insulin-like-growth-factor-binding-protein-1-phosphorylation-in-decidualized-stromal-mesenchymal-cells-in-human-intrauterine-growth-restriction-placentas
#7
Sahil S Singal, Karen Nygard, Robert Gratton, Thomas Jansson, Madhulika B Gupta
Intrauterine growth restriction (IUGR) is often caused by placental insufficiency, which is believed to be associated with decreased delivery of oxygen and nutrients to the placental barrier. We recently reported that hypoxia and/or leucine deprivation triggered hyperphosphorylation of insulin-like growth factor binding protein-1 (IGFBP-1) in decidualized human immortalized endometrial stromal cells (HIESCs), resulting in decreased insulin-like growth factor-1 (IGF-1) bioactivity. To test the hypothesis that human IUGR is associated with increased decidual IGFBP-1 phosphorylation at discrete sites, we used IUGR and gestational age matched appropriate for gestational age (AGA) placentas ( n=5 each)...
May 1, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29715698/antenatal-vitamin-d-preserves-placental-vascular-and-fetal-growth-in-experimental-chorioamnionitis-due-to-intra-amniotic-endotoxin-exposure
#8
Michael W Cookson, Sharon L Ryan, Gregory J Seedorf, R Blair Dodson, Steve H Abman, Erica W Mandell
BACKGROUND:  Chorioamnionitis (CA) is associated with a high risk for the development of bronchopulmonary dysplasia (BPD) after preterm birth, but mechanisms that increase susceptibility for BPD and strategies to prevent BPD are uncertain. As a model of CA, antenatal intra-amniotic (IA) endotoxin (ETX) exposure alters placental structure, causes fetal growth restriction, increases perinatal mortality, and causes sustained cardiorespiratory abnormalities throughout infancy. Vitamin D (Vit D) has been shown to have both anti-inflammatory and proangiogenic properties...
May 1, 2018: American Journal of Perinatology
https://www.readbyqxmd.com/read/29677003/identification-of-a-novel-tcf4-isoform-in-the-human-corneal-endothelium
#9
Allen O Eghrari, Shivakumar Vasanth, Briana C Gapsis, Henry Bison, Ula Jurkunas, S Amer Riazuddin, John D Gottsch
PURPOSE: Alternative splice isoforms of TCF4, a gene implicated in Fuchs corneal dystrophy, have been identified in multiple human tissues outside of the eye. The aim of this study was to identify the transcriptional profile of TCF4 in the corneal endothelium. METHODS: We extracted RNA from the donor corneal endothelium and performed rapid amplification of cDNA ends. We tested the expression pattern of 1 newly identified isoform (7b) in a panel of cDNA derived from multiple human tissues and included cDNA from corneal endothelial (CE) and retinal pigment epithelial cell lines...
April 19, 2018: Cornea
https://www.readbyqxmd.com/read/29673796/cigarette-smoke-extract-interferes-with-placenta-macrophage-functions-a-new-mechanism-to-compromise-placenta-functions
#10
Rym Belhareth, Soraya Mezouar, Amira Ben Amara, Céline Chartier, Eya Ben Azzouz, Eric Chabrière, Mohamed Amri, Jean-Louis Mege
The success of pregnancy depends on the maternal immune system's ability to promote tolerance and host defense. This equilibrium is compromised in inflammatory and infectious impairment of placenta. Smoking during pregnancy exposes the fetus to severe complications which might result from an alteration in placenta macrophages (pMφ) functions. In this study, we assessed the effect of cigarette smoke extract (CSE) on the functions of third trimester pMφs.CSE inhibited particles uptake and the formation of multinucleated giant cells, a recently reported property of pMφs based on their ability to fuse in vitro...
April 16, 2018: Reproductive Toxicology
https://www.readbyqxmd.com/read/29609429/comparison-of-wound-healing-effects-between-oncorhynchus-keta-derived-polydeoxyribonucleotide-pdrn-and-oncorhynchus-mykiss-derived-pdrn
#11
Jong Hun Lee, Jin Woo Han, Jin Hwan Byun, Won Mi Lee, Min Ho Kim, Wen Hao Wu
BACKGROUND: Polydeoxyribonucleotide (PDRN) influencing cellular growth and differentiation is recognized to promote wound healing by stimulating tissue repair. Although PDRN can be extracted from human placentas, PDRN medications have recently been extracted from the semen of trout ( Oncorhynchus mykiss ) and salmon ( Oncorhynchus keta ). The present study was designed to evaluate the wound healing effects of O. keta -derived PDRN for injection (Rejuvenex) and PDRN cream (Rejuvenex Cream) in comparison with those of O...
March 2018: Archives of Craniofacial Surgery
https://www.readbyqxmd.com/read/29602980/placenta-previa-and-placental-abruption-after-assisted-reproductive-technology-in-patients-with-endometriosis-a-systematic-review-and-meta-analysis
#12
Maria Luisa Gasparri, Konstantinos Nirgianakis, Katayoun Taghavi, Andrea Papadia, Michael D Mueller
INTRODUCTION: Recent evidence suggests that assisted reproductive technology (ART) increases the risk of adverse pregnancy outcomes, including placental disorders. Similarly, endometriosis resulted detrimental on placenta previa. However, up to 50% of women with endometriosis suffer from infertility, thus requiring ART. The aim of our metanalysis is to compare women with and without endometriosis undergoing ART in terms of placenta disorders events, to establish if ART itself or endometriosis, as an indication to ART, increases the risk of placenta previa...
March 30, 2018: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29597262/maternal-choline-supplementation-during-normal-murine-pregnancy-alters-the-placental-epigenome-results-of-an-exploratory-study
#13
Sze Ting Cecilia Kwan, Julia H King, Jennifer K Grenier, Jian Yan, Xinyin Jiang, Mark S Roberson, Marie A Caudill
The placental epigenome regulates processes that affect placental and fetal development, and could be mediating some of the reported effects of maternal choline supplementation (MCS) on placental vascular development and nutrient delivery. As an extension of work previously conducted in pregnant mice, the current study sought to explore the effects of MCS on various epigenetic markers in the placenta. RNA and DNA were extracted from placentas collected on embryonic day 15.5 from pregnant mice fed a 1X or 4X choline diet, and were subjected to genome-wide sequencing procedures or mass-spectrometry-based assays to examine placental imprinted gene expression, DNA methylation patterns, and microRNA (miRNA) abundance...
March 28, 2018: Nutrients
https://www.readbyqxmd.com/read/29588451/identification-of-the-functional-pathways-altered-by-placental-cell-exposure-to-high-glucose-lessons-from-the-transcript-and-metabolite-interactome
#14
C H Hulme, A Stevens, W Dunn, A E P Heazell, K Hollywood, P Begley, M Westwood, J E Myers
The specific consequences of hyperglycaemia on placental metabolism and function are incompletely understood but likely contribute to poor pregnancy outcomes associated with diabetes mellitus (DM). This study aimed to identify the functional biochemical pathways perturbed by placental exposure to high glucose levels through integrative analysis of the trophoblast transcriptome and metabolome. The human trophoblast cell line, BeWo, was cultured in 5 or 25 mM glucose, as a model of the placenta in DM. Transcriptomic analysis using microarrays, demonstrated 5632 differentially expressed gene transcripts (≥± 1...
March 27, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29549185/identification-of-a-nf-%C3%AE%C2%BA-b-inhibition-site-on-the-proximal-promoter-region-of-human-organic-anion-transporting-polypeptide-1a2-coding-gene-slco1a2
#15
Zhaojian Xiang, Weike Li, Lixue Wang, Jicai Yi, Kaiwen Chen, Mei Hong
Organic anion transporting polypeptides (OATPs; gene symbol SLCO ) are membrane transporters that mediate the transport of wide ranges of compounds. The expression of different OATP members has been reported in the kidney, liver, placenta, brain, and intestine. Because of their broad substrate spectra and wide distribution within the human body, these transporters have been proposed to play key roles in the influx transport of many oral drugs. Inflammation is known to regulate the expression and functions of many drug-metabolizing enzymes and drug transporters...
May 2018: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/29540242/comparison-of-single-nucleotide-polymorphisms-in-the-3-untranslated-region-of-hla-g-in-placentas-between-spontaneous-preterm-birth-and-preeclampsia
#16
Ji Young Lee, Hyun Mi Kim, Mi Ju Kim, Hyun-Hwa Cha, Won Joon Seong
OBJECTIVE: To compare single nucleotide polymorphisms (SNPs) in the 3'-untranslated region (3'UTR) of human leukocyte antigen (HLA)-G in placentas between spontaneous preterm birth and preeclampsia pregnancies. RESULTS: Placental samples matched for gestational age were obtained from 20 cases of spontaneous preterm births and 19 cases of preeclampsia. Genomic deoxyribonucleic acid was extracted from placenta tissue and the 3'UTR region of HLA-G was amplified via polymerase chain reaction...
March 14, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29535770/placenta-and-placental-derivatives-in-regenerative-therapies-experimental-studies-history-and-prospects
#17
REVIEW
Olena Pogozhykh, Volodymyr Prokopyuk, Constança Figueiredo, Denys Pogozhykh
Placental structures, capable to persist in a genetically foreign organism, are a natural model of allogeneic engraftment carrying a number of distinctive properties. In this review, the main features of the placenta and its derivatives such as structure, cellular composition, immunological and endocrine aspects, and the ability to invasion and deportation are discussed. These features are considered from a perspective that determines the placental material as a unique source for regenerative cell therapies and a lesson for immunological tolerance...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29524345/development-and-validation-of-a-liquid-chromatography-tandem-mass-spectrometry-method-for-the-determination-of-nicotine-and-its-metabolites-in-placenta-and-umbilical-cord
#18
Lucía Paniagua-González, Cristian Jiménez-Morigosa, Elena Lendoiro, Marta Concheiro, Angelines Cruz, Manuel López-Rivadulla, Ana de Castro
Tobacco exposure during pregnancy is associated with obstetric and fetal complications. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine nicotine, cotinine, and hydroxycotinine (OH-cotinine) in placenta (PL) and umbilical cord (UC). Specimens were homogenized in water, followed by solid-phase extraction. Chromatographic separation was performed using an Atlantis® HILIC Silica column. Detection was accomplished in electrospray in positive mode. Method validation included: linearity (5 to 1000 ng/g), accuracy (86...
March 10, 2018: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29510809/aetiology-and-treatment-of-severe-postpartum-haemorrhage
#19
Hellen McKinnon Edwards
This thesis is comprised of three studies focusing on severe postpartum haemorrhage (PPH). PPH is a major cause of maternal morbidity and mortality worldwide. Risk factors include retained placenta, prolonged duration of the third stage of labour, previous caesarean section, and operative vaginal delivery. Occurrence and development of PPH are, however, unpredictable and can sometimes give rise to massive haemorrhage or even hysterectomy and maternal death. Severe haemorrhage can lead to coagulopathy causing further haemorrhage and requiring substitution with blood transfusions...
March 2018: Danish Medical Journal
https://www.readbyqxmd.com/read/29505656/association-of-the-length-of-the-third-stage-of-labor-and-blood-loss-following-vaginal-delivery
#20
Nader Z Rabie, Songthip Ounpraseuth, Dawn Hughes, Patrick Lang, Micah Wiegel, Everett F Magann
OBJECTIVES: The length of the third stage of labor is correlated with blood loss following a vaginal delivery. We aimed to accurately measure blood loss following a vaginal delivery and examine the relation between blood loss and length of the third stage of labor. METHODS: This was a prospective observational study of singleton pregnancies ≥24 weeks undergoing a vaginal delivery. Blood loss was meticulously measured and the length of the third stage of labor was recorded...
March 2018: Southern Medical Journal
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