Yoshitaka Shinno, Hisanori Takenobu, Ryuichi P Sugino, Yuki Endo, Ryu Okada, Masayuki Haruta, Shunpei Satoh, Kyosuke Mukae, Dilibaerguli Shaliman, Tomoko Wada, Jesmin Akter, Kiyohiro Ando, Atsuko Nakazawa, Hideo Yoshida, Miki Ohira, Tomoro Hishiki, Takehiko Kamijo
In the present study, we found that EZH1 depletion in MYCN-amplified neuroblastoma cells resulted in significant cell death as well as xenograft inhibition. EZH1 depletion decreased the level of H3K27me1; the interaction and protein stabilization of MYCN and EZH1 appear to play roles in epigenetic transcriptional regulation. Transcriptome analysis of EZH1-depleted cells resulted in down-regulation of the cell cycle progression-related pathway. In particular, GSEA revealed down-regulation of reactome E2F-mediated regulation of DNA replication along with key genes of this process, TYMS, POLA2, and CCNA1...
September 2, 2022: Cancer Science