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https://www.readbyqxmd.com/read/28411256/epigenetically-induced-ectopic-expression-of-uncx-impairs-the-proliferation-and-differentiation-of-myeloid-cells
#1
Giulia Daniele, Giogia Simonetti, Caterina Fusilli, Ilaria Iacobucci, Angelo Lonoce, Antonio Palazzo, Mariana Lomiento, Fabiana Mammoli, Rene' Massimiliano Marsano, Elena Marasco, Vilma Mantovani, Hilmar Quentmeier, Hans G Drexler, Jie Ding, Orazio Palumbo, Massimo Carella, Niroshan Nadarajah, Margherita Perricone, Emanuela Ottaviani, Carmen Baldazzi, Nicoletta Testoni, Cristina Papayannidis, Sergio Ferrari, Tommaso Mazza, Giovanni Martinelli, Clelia Tiziana Storlazzi
We here describe a leukemogenic role of the homeobox gene UNCX, activated by epigenetic modifications in acute myeloid leukemia. We found the ectopic activation of UNCX in a leukemia patient harboring a t(7;10)(p22;p14) translocation, in 22/61 of additional cases [a total of 23 positive patients out of 62 (37.1%)], and in 6/75 (8%) of acute myeloid leukemia cell lines. UNCX is embedded within a low-methylation region (canyon) and encodes for a transcription factor involved in somitogenesis and neurogenesis, with specific expression in the eye, brain, and kidney...
April 14, 2017: Haematologica
https://www.readbyqxmd.com/read/28301569/meiotic-failure-in-cyclin-a1-deficient-mouse-spermatocytes-triggers-apoptosis-through-intrinsic-and-extrinsic-signaling-pathways-and-14-3-3-proteins
#2
Sunil K Panigrahi, Marcia Manterola, Debra J Wolgemuth
Cyclin A1 (Ccna1), a member of the mammalian A type cyclins, is most abundantly expressed in spermatocytes and is essential for spermatogenesis in the mouse. Ccna1- deficient spermatocytes arrest at late meiotic prophase and undergo apoptosis. To further delineate the mechanisms and key factors involved in this process, we have examined changes in expression of genes involved in both intrinsic and extrinsic signaling pathways that trigger apoptosis in the mutant spermatocytes. Our results show that both pathways are involved, and that the factors involved in the intrinsic pathway were expressed earlier than those involved in the extrinsic pathway...
2017: PloS One
https://www.readbyqxmd.com/read/28285559/identification-and-validation-of-gene-expression-patterns-in-cystitis-glandularis-patients-and-controls
#3
Chen-Long Chu, Chen-Hui Zhao, Zhi-Wei Zhang, Ming-Wei Wang, Zhao-Hui Zhang, An-Qing Yang, Bin-Bin Ma, Cai-Feng Lu, Min Wu, Mei-Zhen Gu, Ren-Jie Cui, Zhi-Xiang Xin, Tao Huang, Wen-Long Zhou
Our aim was to investigate differences in gene expression in bladder tissues between cystitis glandularis (CG) patients and healthy controls. Subsequent RNA was isolated from urinary bladder samples from CG patients and healthy controls, followed by RNA sequencing analysis. There were 4263 differentially expressed genes in urinary bladder between CG patients and controls, and 8 genes were verified with real-time PCR, Western blot, and enzyme-linked immunosorbent assay (ELISA) analysis. Gene set enrichment analysis (GSEA) revealed that 25 signaling pathways were upregulated in CG patients, and 17 signaling pathways were found upregulated in healthy controls...
January 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28231331/mesenchymal-stromal-cells-mscs-induce-ex-vivo-proliferation-and-erythroid-commitment-of-cord-blood-haematopoietic-stem-cells-cb-cd34-cells
#4
Simone Perucca, Andrea Di Palma, Pier Paolo Piccaluga, Claudia Gemelli, Elisa Zoratti, Giulio Bassi, Edoardo Giacopuzzi, Andrea Lojacono, Giuseppe Borsani, Enrico Tagliafico, Maria Teresa Scupoli, Simona Bernardi, Camilla Zanaglio, Federica Cattina, Valeria Cancelli, Michele Malagola, Mauro Krampera, Mirella Marini, Camillo Almici, Sergio Ferrari, Domenico Russo
A human bone marrow-derived mesenchymal stromal cell (MSCs) and cord blood-derived CD34+ stem cell co-culture system was set up in order to evaluate the proliferative and differentiative effects induced by MSCs on CD34+ stem cells, and the reciprocal influences on gene expression profiles. After 10 days of co-culture, non-adherent (SN-fraction) and adherent (AD-fraction) CD34+ stem cells were collected and analysed separately. In the presence of MSCs, a significant increase in CD34+ cell number was observed (fold increase = 14...
2017: PloS One
https://www.readbyqxmd.com/read/28042535/effects-of-mono-2-ethylhexyl-phthalate-and-di-2-ethylhexyl-phthalate-administrations-on-oocyte-meiotic-maturation-apoptosis-and-gene-quantification-in-mouse-model
#5
Forouzan Absalan, Sadegh Saremy, Esrafil Mansori, Mahin Taheri Moghadam, Ali Reza Eftekhari Moghadam, Razie Ghanavati
OBJECTIVE: Phthalates, which are commonly used to render plastics into soft and flexible materials, have also been determined as developmental and reproductive toxicants in human and animals. The purpose of this study was to evaluate the effect of mono-(2- ethylhexyl) phthalate (MEHP) and di-(2-ethylhexyl) phthalate (DEHP) oral administrations on maturation of mouse oocytes, apoptosis and gene transcription levels. MATERIALS AND METHODS: In this experimental study, immature oocytes recovered from Naval Medical Research Institute (NMRI) mouse strain (6-8 weeks), were divided into seven different experimental and control groups...
2017: Cell Journal
https://www.readbyqxmd.com/read/27884605/arsenic-induced-activation-of-the-homeodomain-interacting-protein-kinase-2-hipk2-to-camp-response-element-binding-protein-creb-axis
#6
Kazunori Hashimoto, Yoshiaki Tsuji
Cyclic AMP-response element-binding protein (CREB) plays key transcriptional roles in cell metabolism, proliferation, and survival. Ser133 phosphorylation by protein kinase A (PKA) is a well-characterized CREB activation mechanism. Homeodomain-interacting protein kinase (HIPK) 2, a nuclear serine/threonine kinase, activates CREB through Ser271 phosphorylation; however, the regulatory mechanism remains uncharacterized. Transfection of CREB in HEK293 cells together with the kinase demonstrated that HIPK2 phosphorylated CREB at Ser271 but not Ser133; likewise, PKA phosphorylated CREB at Ser133 but not Ser271, suggesting two distinct CREB regulatory mechanisms by HIPK2 and PKA...
January 6, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/27802200/modern-molecular-biomarkers-of-head-and-neck-cancer-part-i-epigenetic-diagnostics-and-prognostics-systematic-review
#7
REVIEW
Gintaras Juodzbalys, David Kasradze, Marco Cicciù, Aurimas Sudeikis, Laurynas Banys, Pablo Galindo-Moreno, Zygimantas Guobis
INTRODUCTION: Nearly half of the head and neck cancer cases are diagnosed in late stages. Traditional screening modalities have many disadvantages. The aim of the present article was to review the scientific literature about novel head and neck cancer diagnostics - epigenetic biomarkers. EVIDENCE ACQUISITION: A comprehensive review of the current literature was conducted according to the PRISMA guidelines by accessing the NCBI PubMed database. Authors conducted the search of articles in English language published from 2004 to 2015...
2016: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/27669502/cyclin-a1-expression-and-paclitaxel-resistance-in-human-ovarian-cancer-cells
#8
Kuan-Chun Huang, Junzheng Yang, Michelle C Ng, Shu-Kay Ng, William R Welch, Michael G Muto, Ross S Berkowitz, Shu-Wing Ng
BACKGROUND: The development of intrinsic and acquired resistance to antineoplastic agents is a major obstacle to successful chemotherapy in ovarian cancers. Identification and characterisation of chemoresponse-associated biomarkers are of paramount importance for novel therapeutic development. METHODS: Global RNA expression profiles were obtained by high-throughput microarray analysis. Cell cycle, proliferation rate, and paclitaxel sensitivity of ovarian cancer cells harbouring cyclin A1-inducible expression construct were compared with and without tetracycline induction, as well as when the cyclin A1 expression was suppressed by short inhibiting RNA (siRNA)...
November 2016: European Journal of Cancer
https://www.readbyqxmd.com/read/27009662/quantitative-nanomechanical-mapping-of-marine-diatom-in-seawater-using-peak-force-tapping-atomic-force-microscopy-1
#9
Galja Pletikapić, Alexandre Berquand, Tea Mišić Radić, Vesna Svetličić
It is generally accepted that a diatom cell wall is characterized by a siliceous skeleton covered by an organic envelope essentially composed of polysaccharides and proteins. Understanding of how the organic component is associated with the silica structure provides an important insight into the biomineralization process and patterning on the cellular level. Using a novel atomic force microscopy (AFM) imaging technique (Peak Force Tapping), we characterized nanomechanical properties (elasticity and deformation) of a weakly silicified marine diatom Cylindrotheca closterium (Ehrenb...
February 2012: Journal of Phycology
https://www.readbyqxmd.com/read/26969498/photoreceptor-proliferation-and-dysregulation-of-cell-cycle-genes-in-early-onset-inherited-retinal-degenerations
#10
Kristin L Gardiner, Louise Downs, Agnes I Berta-Antalics, Evelyn Santana, Gustavo D Aguirre, Sem Genini
BACKGROUND: Mitotic terminally differentiated photoreceptors (PRs) are observed in early retinal degeneration (erd), an inherited canine retinal disease driven by mutations in the NDR kinase STK38L (NDR2). RESULTS: We demonstrate that a similar proliferative response, but of lower magnitude, occurs in two other early onset disease models, X-linked progressive retinal atrophy 2 (xlpra2) and rod cone dysplasia 1 (rcd1). Proliferating cells are rod PRs, and not microglia or Müller cells...
March 11, 2016: BMC Genomics
https://www.readbyqxmd.com/read/26921336/cyclin-a1-and-p450-aromatase-promote-metastatic-homing-and-growth-of-stem-like-prostate-cancer-cells-in-the-bone-marrow
#11
Regina Miftakhova, Andreas Hedblom, Julius Semenas, Brian Robinson, Athanasios Simoulis, Johan Malm, Albert Rizvanov, David M Heery, Nigel P Mongan, Norman J Maitland, Cinzia Allegrucci, Jenny L Persson
Bone metastasis is a leading cause of morbidity and mortality in prostate cancer. While cancer stem-like cells have been implicated as a cell of origin for prostate cancer metastasis, the pathways that enable metastatic development at distal sites remain largely unknown. In this study, we illuminate pathways relevant to bone metastasis in this disease. We observed that cyclin A1 (CCNA1) protein expression was relatively higher in prostate cancer metastatic lesions in lymph node, lung, and bone/bone marrow. In both primary and metastatic tissues, cyclin A1 expression was also correlated with aromatase (CYP19A1), a key enzyme that directly regulates the local balance of androgens to estrogens...
April 15, 2016: Cancer Research
https://www.readbyqxmd.com/read/26867769/association-between-the-cpg-island-methylator-phenotype-and-its-prognostic-significance-in-primary-pulmonary-adenocarcinoma
#12
Young Wha Koh, Sung-Min Chun, Young-Soo Park, Joon Seon Song, Geon Kook Lee, Shin Kwang Khang, Se Jin Jang
Aberrant methylation of promoter CpG islands is one of the most important inactivation mechanisms for tumor suppressor and tumor-related genes. Previous studies using genome-wide DNA methylation microarray analysis have suggested the existence of a CpG island methylator phenotype (CIMP) in lung adenocarcinomas. Although the biological behavior of these tumors varies according to tumor stage, no large-scale study has examined the CIMP in lung adenocarcinoma patients according to tumor stage. Furthermore, there have been no reported results regarding the clinical significance of each of the six CIMP markers...
August 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/26744873/dysregulated-module-approach-identifies-disrupted-genes-and-pathways-associated-with-acute-myelocytic-leukemia
#13
Y Fang, L-N Xie, X-M Liu, Z Yu, F-S Kong, N-X Song, F Zhou
OBJECTIVE: To identify disrupted genes and pathways involved in acute myelocytic leukemia (AML) by systematically tracking the dysregulated modules across normal and AML conditions. MATERIALS AND METHODS: In this study, we firstly integrated the protein interaction data and expression profiles to infer and reweight the normal and AML networks using Pearson correlation coefficient (PCC). Next, clustering-based on maximal cliques (CMC) approach and a maximum weight bipartite matching method were implemented to infer the condition-specific modules and capture the disturbed modules, respectively, from two conditional networks...
December 2015: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/26686387/re-expression-of-selected-epigenetically-silenced-candidate-tumor-suppressor-genes-in-cervical-cancer-by-tet2-directed-demethylation
#14
Christian Huisman, Monique G P van der Wijst, Matthijs Schokker, Pilar Blancafort, Martijn M Terpstra, Klaas Kok, Ate G J van der Zee, Ed Schuuring, G Bea A Wisman, Marianne G Rots
DNA hypermethylation is extensively explored as therapeutic target for gene expression modulation in cancer. Here, we re-activated hypermethylated candidate tumor suppressor genes (TSGs) (C13ORF18, CCNA1, TFPI2, and Maspin) by TET2-induced demethylation in cervical cancer cell lines. To redirect TET2 to hypermethylated TSGs, we engineered zinc finger proteins (ZFPs), which were first fused to the transcriptional activator VP64 to validate effective gene re-expression and confirm TSG function. ChIP-Seq not only revealed enriched binding of ZFPs to their intended sequence, but also considerable off-target binding, especially at promoter regions...
March 2016: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/26623041/diagnostic-value-of-cyclin-dependent-kinase-cyclin-dependent-kinase-inhibitor-expression-ratios-as-biomarkers-of-locoregional-and-hematogenous-dissemination-risks-in-oral-squamous-cell-carcinoma
#15
Masaki Nagata, Hiroshi Kurita, Kohya Uematsu, Shin Ogawa, Katsu Takahashi, Hideyuki Hoshina, Ritsuo Takagi
The aim of the present study was to investigate the diagnostic value of cell cycle-related genes in oral squamous cell carcinoma (OSCC) by examining the expression of the following genes in 77 OSCC tissues by quantitative polymerase chain reaction: Cyclin genes (CCNA1, CCND1, CCND2 and CCNE1), cyclin-dependent kinase (CDK) genes (CDK1, CDK2 and CDK4), CDK inhibitor genes (CDKN2A, CDKN1A, CDKN1B and CDKN1C), and integrin and associated genes that we previously reported (ITGA3, ITGB4, CD9 and JUP). The expression ratios of 66 combinations of the 11 cell cycle-related genes were analyzed to examine their associations with major clinical events using Mann-Whitney U and log-rank tests...
September 2015: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/26566865/hmgn1-acts-downstream-of-c-ebp%C3%AE-to-regulate-the-decidualization-of-uterine-stromal-cells-in-mice
#16
Dang-Dang Li, Zhan-Qing Yang, Chuan-Hui Guo, Liang Yue, Cui-Cui Duan, Hang Cao, Bin Guo, Zhan-Peng Yue
Although Hmgn1 is involved in the regulation of gene expression and cellular differentiation, its physiological roles on the differentiation of uterine stromal cells during decidualization still remain unknown. Here we showed that Hmgn1 mRNA was highly expressed in the decidua on days 6-8 of pregnancy. Simultaneously, increased expression of Hmgn1 was also observed in the artificial and in vitro induced decidualization models. Hmgn1 induced the proliferation of uterine stromal cells and expression of Ccna1, Ccnb1, Ccnb2 and Cdk1 in the absence of estrogen and progesterone...
2015: Cell Cycle
https://www.readbyqxmd.com/read/26566081/stability-of-methylation-markers-in-head-and-neck-squamous-cell-carcinoma
#17
Shama Virani, Emily Light, Lisa A Peterson, Maureen A Sartor, Jeremy M G Taylor, Jonathan B McHugh, Gregory T Wolf, Laura S Rozek
BACKGROUND: As cancer progresses, methylation patterns change to promote the tumorigenic phenotype. However, stability of methylation markers over time and the extent that biopsy samples are representative of larger tumor specimens are unknown. This information is critical for clinical use of such biomarkers. METHODS: Ninety-eight patients with tumor specimens from 2 timepoints were measured for DNA methylation in the promoter regions across 4 genes. RESULTS: There were no significant differences in overall methylation of CCNA1 (cyclin A1), NDN (necdin), deleted in colorectal carcinoma (DCC), and cluster of differentiation 1a (CD1A) within paired specimens (p values = ...
April 2016: Head & Neck
https://www.readbyqxmd.com/read/26518708/ndn-and-cd1a-are-novel-prognostic-methylation-markers-in-patients-with-head-and-neck-squamous-carcinomas
#18
Shama Virani, Emily Bellile, Carol R Bradford, Thomas E Carey, Douglas B Chepeha, Justin A Colacino, Joseph I Helman, Jonathan B McHugh, Lisa A Peterson, Maureen A Sartor, Jeremy Mg Taylor, Heather M Walline, Greg T Wolf, Laura S Rozek
BACKGROUND: HPV-associated HNSCCs have a distinct etiologic mechanism and better prognosis than those with non-HPV associated HNSCCs. However, even within the each group, there is heterogeneity in survival time. Here, we test the hypothesis that specific candidate gene methylation markers (CCNA1, NDN, CD1A, DCC, p16, GADD45A) are associated with tumor recurrence and survival, in a well-characterized, prospective, cohort of 346 HNSCC patients. METHODS: Kaplan-Meier curves were used to estimate survival time distributions...
October 30, 2015: BMC Cancer
https://www.readbyqxmd.com/read/26517242/jam3-methylation-status-as-a-biomarker-for-diagnosis-of-preneoplastic-and-neoplastic-lesions-of-the-cervix
#19
COMPARATIVE STUDY
Aijun Yin, Qing Zhang, Xiangnan Kong, Lin Jia, Ziyan Yang, Lihua Meng, Li Li, Xiao Wang, Yunbo Qiao, Nan Lu, Qifeng Yang, Keng Shen, Beihua Kong
DNA methylation is clinically relevant to important tumorigenic mechanisms. This study evaluated the methylation status of candidate genes in cervical neoplasia and determined their diagnostic performance in clinical practice. Cervical cancer and normal cervix tissue was used to select the top 5 discriminating loci among 27 loci in 4 genes (CCNA1, CADM1, DAPK1, JAM3), and one locus of JAM3 (region M4) was identified and confirmed with 267 and 224 cervical scrapings from 2 independent colposcopy referral studies...
December 29, 2015: Oncotarget
https://www.readbyqxmd.com/read/26447864/exclusive-association-of-p53-mutation-with-super-high-methylation-of-tumor-suppressor-genes-in-the-p53-pathway-in-a-unique-gastric-cancer-phenotype
#20
Mina Waraya, Keishi Yamashita, Akira Ema, Natsuya Katada, Shiro Kikuchi, Masahiko Watanabe
BACKGROUND: A comprehensive search for DNA methylated genes identified candidate tumor suppressor genes that have been proven to be involved in the apoptotic process of the p53 pathway. In this study, we investigated p53 mutation in relation to such epigenetic alteration in primary gastric cancer. METHODS: The methylation profiles of the 3 genes: PGP9.5, NMDAR2B, and CCNA1, which are involved in the p53 tumor suppressor pathway in combination with p53 mutation were examined in 163 primary gastric cancers...
2015: PloS One
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