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https://www.readbyqxmd.com/read/29351348/interactions-of-2-o-methyl-oligoribonucleotides-with-the-rna-models-of-the-30s-subunit-a-site
#1
Maciej Jasiński, Marta Kulik, Monika Wojciechowska, Ryszard Stolarski, Joanna Trylska
Synthetic oligonucleotides targeting functional regions of the prokaryotic rRNA could be promising antimicrobial agents. Indeed, such oligonucleotides were proven to inhibit bacterial growth. 2'-O-methylated (2'-O-Me) oligoribonucleotides with a sequence complementary to the decoding site in 16S rRNA were reported as inhibitors of bacterial translation. However, the binding mode and structures of the formed complexes, as well as the level of selectivity of the oligonucleotides between the prokaryotic and eukaryotic target, were not determined...
2018: PloS One
https://www.readbyqxmd.com/read/29350745/tio2-nanoparticles-can-selectively-bind-cxcl8-impacting-on-neutrophil-chemotaxis
#2
J Batt, M Milward, I Chapple, M Grant, H Roberts, O Addison
The interaction between TiO2 nanoparticles (NPs) and inflammatory cytokines, including CXCL8, a clinically relevant pro-inflammatory chemokine was investigated. TiO2 is present in tissues adjacent to failing implanted Ti (titanium) devices. TiO2 NPs were shown to bind to CXCL8 in vitro, causing perturbation of quantification of CXCL8 by ELISA, in both simple and complex protein panels, in a dose-dependent manner. Binding between TiO2 NPs and CXCL8 was demonstrated by protein gel electrophoresis. TiO2 NPs were also shown to inactivate the chemoattractant property of CXCL8 in a dose-dependent manner, suggesting that the binding between TiO2 NPs and CXCL8 is likely to be clinically relevant...
January 19, 2018: European Cells & Materials
https://www.readbyqxmd.com/read/29350293/diversity-and-enzymatic-potentialities-of-bacillus-sp-strains-isolated-from-a-polluted-freshwater-ecosystem-in-cuba
#3
Jeny Adina Larrea-Murrell, Marcia Maria Rojas-Badia, Ivette García-Soto, Beatriz Romeu-Alvarez, Tristano Bacchetti, Annika Gillis, Ana Karina Boltes-Espinola, Mayra Heydrich-Perez, Daysi Lugo-Moya, Jacques Mahillon
Genotypic and phenotypic characterization of Bacillus spp. from polluted freshwater has been poorly addressed. The objective of this research was to determine the diversity and enzymatic potentialities of Bacillus spp. strains isolated from the Almendares River. Bacilli strains from a polluted river were characterized by considering the production of extracellular enzymes using API ZYM. 14 strains were selected and identified using 16S rRNA, gyrB and aroE genes. Genotypic diversity of the Bacillus spp. strains was evaluated using pulsed field gel electrophoresis...
January 19, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29345939/discovery-of-alternative-producers-of-the-enediyne-antitumor-antibiotic-c-1027-with-high-titers
#4
Xiaohui Yan, Hindra, Huiming Ge, Dong Yang, Tingting Huang, Ivana Crnovcic, Chin-Yuan Chang, Shi-Ming Fang, Thibault Annaval, Xiangcheng Zhu, Yong Huang, Li-Xing Zhao, Yi Jiang, Yanwen Duan, Ben Shen
The potent cytotoxicity and unique mode of action make the enediyne antitumor antibiotic C-1027 an exquisite drug candidate for anticancer chemotherapy. However, clinical development of C-1027 has been hampered by its low titer from the original producer Streptomyces globisporus C-1027. Here we report three new C-1027 alternative producers, Streptomyces sp. CB00657, CB02329, and CB03608, from The Scripps Research Institute actinomycetes strain collection. Together with the previously disclosed Streptomyces sp...
January 18, 2018: Journal of Natural Products
https://www.readbyqxmd.com/read/29345567/proteomic-analysis-of-vernalization-responsive-proteins-in-winter-wheat-jing841
#5
Yalan Feng, Beibei Kong, Jun Zhang, Xianni Chen, Jiale Yuan, Xingwang Tang, Chao Ma
Vernalization is one of the pivotal ways for plants to flower. As one of the major cereal crops, wheat is widely planted in the world. In the present study, we applied two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS) to analyze the changes in protein expression profiles in responding to vernalization in leaves of wheat seedling before (0d) and after (30d) of vernalization. The results indicated that among the 68 differentially expressed proteins that were successfully identified under vernalization, 36 were up-regulated whereas 32 were down-regulated at 0 d, respectively...
January 17, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29344948/glycation-of-ovalbumin-after-high-intensity-ultrasound-pretreatment-effects-on-conformation-igg-ige-binding-ability-and-antioxidant-activity
#6
Wenhua Yang, Zongcai Tu, Hui Wang, Lu Zhang, Qidong Song
BACKGROUND: Ovalbumin (OVA), a protein with excellent nutritional and processing properties, is the major allergen of hen egg white. Previous study showed that high-intensity ultrasound treatment increased the IgG and IgE binding abilities by unfolding the conformational structure of OVA. It may allow modifying the IgG and IgE binding of OVA by combining high-intensity ultrasound with other methods, such as glycation, a promising method for the improvement of protein properties. RESULTS: Glycation with mannose (M) after ultrasound pretreatment at 0-600 W significantly reduced the IgG and IgE binding abilities and dramatically enhanced the antioxidant activity of OVA-M conjugates, with the lowest values of IgG and IgE binding and highest values of antioxidant capacity observed at 600W...
January 18, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29344937/ndufaf3-variants-that-disrupt-mitochondrial-complex-i-assembly-may-associate-with-cavitating-leukoencephalopathy
#7
A Ishiyama, K Muramatsu, S Uchino, C Sakai, Y Matsushima, N Makioka, T Ogata, E Suzuki, H Komaki, M Sasaki, M Mimaki, Y-I Goto, I Nishino
Genetic abnormalities in mitochondrial complex assembling factors are associated with leukoencephalopathy. We present a one-year-old girl with consciousness disturbance after a respiratory infection. Brain MRI revealed leukoencephalopathy with bilaterally symmetrical hyperintensity in the substantia nigra, medial thalamic nuclei, and basal nuclei, as well as cavities in the cerebral white matter and corpus callosum. Lactate levels in the spinal fluid were high, while magnetic resonance spectroscopy of the cerebral white matter and basal nuclei showed high peak lactate levels, suggesting mitochondrial dysfunction...
January 17, 2018: Clinical Genetics
https://www.readbyqxmd.com/read/29344855/protocol-for-the-analysis-of-laser-capture-microdissected-fresh-frozen-tissue-homogenates-by-silver-stained-1d-sds-page
#8
DaRue A Prieto, Gordon Whitely, Donald J Johann, Josip Blonder
The heterogeneity present in solid tumors adds significant difficulty to scientific analysis and improved understanding. Fundamentally, solid tumor formation consists of cancer cells proper along with stromal elements. The burgeoning malignant process is dependent upon modified stromal elements. Collectively, the stroma forms an essential microenvironment, which is indispensable for the survival and growth of the malignant neoplasm. This cellular heterogeneity makes molecular profiling of solid tumors via mass spectrometry (MS)-based proteomics a daunting task...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29344805/salivary-biomarkers-for-oral-cancer-and-pre-cancer-screening-a-review
#9
REVIEW
J Kaur, R Jacobs, Y Huang, N Salvo, C Politis
OBJECTIVE: The objective of the study was to conduct a systematic review of the literature assessing potential salivary biomarkers of oral cancer and pre-cancer and discuss emerging issues and challenges in relation to oral cancer and pre-cancer diagnostics. MATERIALS AND METHODS: Search for articles involved the Medline, PubMed, and EMBASE. Specific terms were used from January 1995 to March 2017 by three experts. RESULTS: This search collected 270 articles, of which 105 articles such as reviews, case reports, news, letter to editor, etc...
January 17, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29344361/platelet-protein-biomarker-panel-for-ovarian-cancer-diagnosis
#10
Marta Lomnytska, Rui Pinto, Susanne Becker, Ulla Engström, Sonja Gustafsson, Christina Björklund, Markus Templin, Jan Bergstrand, Lei Xu, Jerker Widengren, Elisabeth Epstein, Bo Franzén, Gert Auer
Background: Platelets support cancer growth and spread making platelet proteins candidates in the search for biomarkers. Methods: Two-dimensional (2D) gel electrophoresis, Partial Least Squares Discriminant Analysis (PLS-DA), Western blot, DigiWest. Results: PLS-DA of platelet protein expression in 2D gels suggested differences between the International Federation of Gynaecology and Obstetrics (FIGO) stages III-IV of ovarian cancer, compared to benign adnexal lesions with a sensitivity of 96% and a specificity of 88%...
2018: Biomarker Research
https://www.readbyqxmd.com/read/29343880/novel-application-of-loop-mediated-isothermal-amplification-for-rapid-detection-of-gene-translocation
#11
Ibu Matsuzaki, Hideto Iguchi, Yurina Mikasa, Hiromu Morishita, Katsuya Okuda, Keita Nakaguchi, Yuki Mori, Yoshifumi Iwahashi, Kenji Warigaya, Masakazu Fujimoto, Fumiyoshi Kojima, Shin-Ichi Murata
Identification of fusion genes in cancer is essential for pathological diagnosis and clinical therapy. Although methods for detection of fusion genes, such as fluorescence in situ hybridization (FISH) and real-time polymerase chain reaction (PCR), have been developed in last two decades, these methods are not ideal for detection of these genetic alterations owing to their high cost and time-consuming procedures. In this study, we developed novel application for detection of gene translocations using loop-mediated isothermal amplification (LAMP)...
December 26, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29343665/molecular-characterization-of-gliadins-of-chinese-spring-wheat-in-relation-to-celiac-disease-elicitors
#12
Kanako Kawaura, Mayuko Miura, Yoko Kamei, Tatsuya M Ikeda, Yasunari Ogihara
The wheat seed storage proteins gliadin and glutenin are encoded by multigenes. Gliadins are further classified into α-, γ-, δ- and ω-gliadins. Genes encoding α-gliadins belong to a large multigene family, whose members are located on the homoeologous group 6 chromosomes at the Gli-2 loci. Genes encoding other gliadins are located on the homoeologous group 1 chromosomes at the Gli-1 loci. Two-dimensional polyacrylamide gel electrophoresis (2-DE) was used to characterize and profile the gliadins. The gliadins in aneuploid Chinese Spring wheat lines were then compared in this study...
January 17, 2018: Genes & Genetic Systems
https://www.readbyqxmd.com/read/29342895/optimization-of-extraction-conditions-and-characterization-of-pepsin-solubilised-collagen-from-skin-of-giant-croaker-nibea-japonica
#13
Fangmiao Yu, Chuhong Zong, Shujie Jin, Jiawen Zheng, Nan Chen, Ju Huang, Yan Chen, Fangfang Huang, Zuisu Yang, Yunping Tang, Guofang Ding
In the present study, response surface methodology was performed to investigate the effects of extraction parameters on pepsin-solubilised collagen (PSC) from the skin of the giant croaker Nibea japonica. The optimum extraction conditions of PSC were as follows: concentration of pepsin was 1389 U/g, solid-liquid ratio was 1:57 and hydrolysis time was 8.67 h. Under these conditions, the extraction yield of PSC was up to 84.85%, which is well agreement with the predict value of 85.03%. The PSC from Nibea japonica skin was then characterized as type I collagen by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...
January 14, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29342414/bacilli-community-of-saline-alkaline-soils-from-the-ararat-plain-armenia-assessed-by-molecular-and-culture-based-methods
#14
Hovik Panosyan, Anna Hakobyan, Nils-Kåre Birkeland, Armen Trchounian
The bacterial community composition in the A horizon of a natural saline-alkaline soil located in Ararat Plain (Armenia) was studied using molecular and culture-based methods The sequence analysis of a 16S rRNA gene clone library and denaturing gradient gel electrophoresis (DGGE) profiles indicated dominance of Firmicutes populations. The majority of the sequences of the bacterial 16S rRNA gene library were close relatives of representatives belonging to the genera Halobacillus (41.2%), Piscibacillus (23.5%), Bacillus (23...
January 5, 2018: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/29342075/enrichment-and-identification-of-the-most-abundant-zinc-binding-proteins-in-developing-barley-grains-by-zinc-imac-capture-and-nano-lc-ms-ms
#15
Giuseppe Dionisio, Mohammad Nasir Uddin, Eva Vincze
Background: Zinc accumulates in the embryo, aleurone, and subaleurone layers at different amounts in cereal grains. Our hypothesis is that zinc could be stored bound, not only to low MW metabolites/proteins, but also to high MW proteins as well. Methods: In order to identify the most abundant zinc binding proteins in different grain tissues, we microdissected barley grains into (1) seed coats; (2) aleurone/subaleurone; (3) embryo; and (4) endosperm. Initial screening for putative zinc binding proteins from the different tissue types was performed by fractionating proteins according to solubility (Osborne fractionation), and resolving those via Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) followed by polyvinylidene fluoride (PVDF) membrane blotting and dithizone staining...
January 17, 2018: Proteomes
https://www.readbyqxmd.com/read/29341915/influence-of-ligands-property-and-particle-size-of-gold-nanoparticles-on-the-protein-adsorption-and-corresponding-targeting-ability
#16
Wei Xiao, Jingyuan Xiong, Shuang Zhang, Yang Xiong, Huajin Zhang, Huile Gao
Nanoparticulated vesicles were widely used for carriers of drugs and imaging probes. To improve the targeting delivery efficiency of these vesicles, ligands were often functionalized onto their surfaces. However, the interaction between vesicles and plasma proteins may cover the ligands and hinder the targeting delivery. It is important to address the potential influence of ligands modification on plasma protein adsorption and the following targeting delivery. In this study, two common used ligands were chosen as the model: transferrin and RGD peptide...
January 13, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29341505/stability-integrity-and-recovery-rate-of-cellular-nucleic-acids-preserved-in-a-new-liquid-based-cytology-medium
#17
Guodong Zhao, Zhaocheng Liu, Yaping Tian, Minsheng Zhu, Shouli Wang, Hui Wang, Shen Gao, Yong Ma, Minxue Zheng
BACKGROUND: Liquid-based cytology (LBC) has replaced the conventional Papanicolaou test in cervical cancer screening. The cervical swab specimens collected in LBC media can also be used for additional analyses including high-risk HPV (HR-HPV) test, DNA methylation analysis, and HPV E6/E7 mRNA test. METHODS: The stability, integrity, and recovery rate of cellular DNA and RNA after storage at different conditions were evaluated by a quantitative real-time PCR (qPCR) based HR-HPV test, reverse transcription qPCR (RT-qPCR), and agarose gel electrophoresis...
January 16, 2018: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29341140/lipoprotein-modifications-by-gingipains-of-porphyromonas-gingivalis
#18
J Lönn, S Ljunggren, K Klarström-Engström, I Demirel, T Bengtsson, H Karlsson
BACKGROUND AND OBJECTIVE: Several studies have shown an association between periodontitis and cardiovascular disease (CVD). Atherosclerosis is the major cause of CVD, and a key event in the development of atherosclerosis is accumulation of lipoproteins within the arterial wall. Bacteria are the primary etiologic agents in periodontitis and Porphyromonas gingivalis is the major pathogen in the disease. Several studies support a role of modified low-density lipoprotein (LDL) in atherogenesis; however, the pathogenic stimuli that induce the changes and the mechanisms by which this occur are unknown...
January 17, 2018: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29340859/comparative-proteomics-of-chromium-transformed-beas-2b-cells-by-2d-dige-and-maldi-tof-tof-ms
#19
Jian Lu, Miaomiao Tang, Yi Liu, Jin Wang, Zhanao Wu
Chromium (Cr) is a highly toxic, common heavy metal used in industrial production. There are two types of Cr in nature: hexavalent chromium (Cr(VI)) and chromium trichloride (Cr(III)). Cr(III) is involved in the metabolism of sugars and lipids, whereas Cr(VI) is absorbed through the respiratory tract and skin and generates free radicals that result in secondary toxicity. Cr(VI) leads to cancer in the occupational population and is therefore recognized as a human carcinogen by the International Agency for Research on Cancer...
January 16, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29340527/cardiac-protein-expression-patterns-are-associated-with-distinct-inborn-exercise-capacity-in-non-selectively-bred-rats
#20
L P Ribeiro, L C Freitas-Lima, G B Naumann, S S Meyrelles, W Lunz, S F Pires, H M Andrade, J B T Carnielli, S G Figueiredo
In the present study, we successfully demonstrated for the first time the existence of cardiac proteomic differences between non-selectively bred rats with distinct intrinsic exercise capacities. A proteomic approach based on two-dimensional gel electrophoresis coupled to mass spectrometry was used to study the left ventricle (LV) tissue proteome of rats with distinct intrinsic exercise capacity. Low running performance (LRP) and high running performance (HRP) rats were categorized by a treadmill exercise test, according to distance run to exhaustion...
January 11, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
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