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Gel electrophoresis

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https://www.readbyqxmd.com/read/28941388/high-frequency-of-s-aureus-mssa-mrsa-in-children-under-1-year-old-with-skin-and-soft-tissue-infections
#1
Lorena Salazar, Judy Natalia Jimenez Quiceno
OBJECTIVE: Staphylococcus aureus is responsible for a large number of infections in pediatric population; however, information about the behavior of such infections in this population is limited. The aim of the study was to describe the clinical, epidemiological, and molecular characteristics of infections caused by methicillin-susceptible and resistant S. aureus (MSSA-MRSA) in a pediatric population. METHOD: A cross-sectional descriptive study in patients from birth to 14 years of age from three high-complexity institutions was conducted (2008-2010)...
September 20, 2017: Jornal de Pediatria
https://www.readbyqxmd.com/read/28938767/isothermal-gelation-behavior-of-myofibrillar-proteins-from-white-and-red-chicken-meat-at-different-temperatures
#2
S Glorieux, L Steen, H Paelinck, I Foubert, I Fraeye
This paper provides insights in the isothermal gelation behavior of white and red chicken myofibrillar proteins (CMP) at different temperatures (20 to 80°C) and the underlying aggregation mechanism, allowing understanding of structure formation in poultry products during thermal processing. At low temperatures (20 to 60°C), an increase in aromatic surface hydrophobicity (SoANS) was found, suggesting potential formation of hydrophobic interactions between CMP. At higher temperatures (60 to 80°C), high SoANS and a significant decrease in total sulfhydryl amount (SH-amount) strongly indicate the presence of hydrophobic interactions and disulfide bonding, resulting in aggregation, as suggested by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...
October 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28938539/dissemination-of-macrolides-fusidic-acid-and-mupirocin-resistance-among-staphylococcus-aureus-clinical-isolates
#3
Xingmei Liu, Shanshan Deng, Jinwei Huang, Yaling Huang, Yu Zhang, Qin Yan, Yanhong Wang, Yanyue Li, Chengfu Sun, Xu Jia
As an increasingly common cause of skin infections worldwide, the prevalence of antibiotic-resistant Staphylococcus aureus (S. aureus) across China has not been well documented. This literature aims to study the resistance profile to commonly used antibiotics, including macrolides, fusidic acid (FA) and mupirocin, and its relationship to the genetic typing in 34 S. aureus strains, including 6 methicillin-resistant S. aureus (MRSA), isolated from a Chinese hospital. The MIC results showed 27 (79.4%), 1 (2.9%) and 6 (17...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28937612/houttuynia-cordata-facilitates-metformin-on-ameliorating-insulin-resistance-associated-with-gut-microbiota-alteration-in-oletf-rats
#4
Jing-Hua Wang, Shambhunath Bose, Soo-Kyoung Lim, AbuZar Ansari, Young-Won Chin, Han Seok Choi, Hojun Kim
Metformin and Houttuynia cordata are representative anti-diabetic therapeutics in western and oriental medicine, respectively. The current study examined the synergistic anti-diabetic effect of Houttuynia cordata extraction (HCE) and metformin combination in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Fecal microbiota were analyzed by denaturing gradient gel electrophoresis (DGGE) and real-time PCR. Combining HCE + metformin resulted in significantly ameliorated glucose tolerance (oral glucose tolerance test (OGTT))-the same as metformin alone...
September 22, 2017: Genes
https://www.readbyqxmd.com/read/28936772/proteome-characterization-of-copper-stress-responses-in-the-roots-of-sorghum
#5
Swapan Kumar Roy, Seong-Woo Cho, Soo Jeong Kwon, Abu Hena Mostafa Kamal, Dong-Gi Lee, Kabita Sarker, Moon-Soon Lee, Zhanguo Xin, Sun-Hee Woo
Copper (Cu) is a important micronutrient for plants, but it is extremely toxic to plants at high concentration and can inactivate and disturb protein structures. To explore the Cu stress-induced tolerance mechanism, the present study was conducted on the roots of sorghum seedlings exposed to 50 and 100 µM CuSO4 for 5 days. Accumulation of Cu increased in roots when the seedlings were treated with the highest concentration of Cu(2+) ions (100 μM). Elevated Cu concentration provoked notable reduction of Fe, Zn, Ca, and Mn uptake in the roots of sorghum seedlings...
September 21, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/28936573/evaluation-of-beauveria-bassiana-infection-in-the%C3%A2-hemolymph-serum-proteins-of-the-housefly-musca-domestica-l-diptera-muscidae
#6
Sapna Mishra, Peeyush Kumar, Anushree Malik
Beauveria bassiana plays a prominent role in biocontrol of houseflies, Musca domestica (L.). Thus, a deeper insight into immune response of M. domestica during B. bassiana infection was warranted to assist the production of more efficient mycoinsecticides. The present study investigates changes in protein profile of M. domestica hemolymph serum post B. bassiana infection using two-dimensional difference gel electrophoresis (2D-DIGE) followed by identification of selected proteins by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS)...
September 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28935871/evaluation-of-biochars-from-different-stock-materials-as-carriers-of-bacterial-strain-for-remediation-of-heavy-metal-contaminated-soil
#7
Ting Wang, Hongwen Sun, Xinhao Ren, Bing Li, Hongjun Mao
Two kinds of biochars, one derived from corn straw and one from pig manure, were studied as carriers of a mutant genotype from Bacillus subtilis (B38) for heavy metal contaminated soil remediation. After amendment with biochar, the heavy metal bioavailability decreased. Moreover, the heavy metal immobilization ability of the biochar was enhanced by combining it with B38. The simultaneous application of B38 and pig manure-derived biochar exhibited a superior effect on the promotion of plant growth and the immobilization of heavy metals in soil...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28935523/an-outbreak-of-burkholderia-cepacia-pseudobacteraemia-caused-by-intrinsically-contaminated-commercial-0-5-chlorhexidine-solution-in-neonatal-intensive-care-units
#8
Je Eun Song, Yee Gyung Kwak, Tae Hyun Um, Chong Rae Cho, Sollip Kim, In Sook Park, Jong Hee Hwang, Namhee Kim, Gang-Bok Oh
BACKGROUND: Burkholderia cepacia is intrinsically resistant to certain antiseptics. We experienced a sudden increase in the frequency of isolation of B. cepacia from blood cultures in a neonatal intensive care unit (NICU) of a university-affiliated hospital. AIM: An investigation was conducted to identify the source and to intervene in the ongoing infections. METHODS: The cases were defined as patients with positive blood cultures for B. cepacia in a NICU between November 2014 and January 2015...
September 18, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28934501/model-based-design-of-rna-hybridization-networks-implemented-in-living-cells
#9
Guillermo Rodrigo, Satya Prakash, Shensi Shen, Eszter Majer, José-Antonio Daròs, Alfonso Jaramillo
Synthetic gene circuits allow the behavior of living cells to be reprogrammed, and non-coding small RNAs (sRNAs) are increasingly being used as programmable regulators of gene expression. However, sRNAs (natural or synthetic) are generally used to regulate single target genes, while complex dynamic behaviors would require networks of sRNAs regulating each other. Here, we report a strategy for implementing such networks that exploits hybridization reactions carried out exclusively by multifaceted sRNAs that are both targets of and triggers for other sRNAs...
September 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28934288/purification-of-rabbit-serum-histidine-proline-rich-glycoprotein-via-preparative-gel-electrophoresis-and-characterization-of-its-glycosylation-patterns
#10
Anna Katharina Weyrauch, Mario Jakob, Angelika Schierhorn, Ralf Bernd Klösgen, Dariush Hinderberger
Histidine-Proline-rich Glycoprotein (HPRG) is a plasma protein of vertebrates and several marine bivalves. Due to its multidomain structure consisting of several regions HPRG can interact with a variety of ligands, however the exact physiological role has not been discovered yet. Past purification approaches out of plasma or serum often led to co-purification of other proteins so that for a profound understanding of the function it is important to obtain a protein of high purity. Recent purification strategies were based upon metale chelate affinity chromatography followed by anion exchange chromatography or size exclusion chromatography, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28934029/comparative-proteomics-of-streptococcus-pneumoniae-response-to-vancomycin-treatment
#11
Xiao-Yan Yang, Jing-Yu Xu, Qiu-Xia Wei, Xuesong Sun, Qing-Yu He
Streptococcus pneumoniae is a gram-positive pathogen that causes otitis media, pneumonia, meningitis, and other serious diseases. Vancomycin is one of the most important drugs currently used for the treatment of gram-positive bacterial infections, representing, importantly, the last line of defense against bacteria that have developed resistance to other antibiotics. While primary efforts of most investigations focused on the antibacterial mechanism of vancomycin, few studies have been performed to assess the tolerance mechanism of bacteria to vancomycin...
September 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28933779/genetically-encoded-releasable-photo-cross-linking-strategies-for-studying-protein-protein-interactions-in-living-cells
#12
Yi Yang, Haiping Song, Dan He, Shuai Zhang, Shizhong Dai, Xiao Xie, Shixian Lin, Ziyang Hao, Huangtao Zheng, Peng R Chen
Although protein-protein interactions (PPIs) have crucial roles in virtually all cellular processes, the identification of more transient interactions in their biological context remains challenging. Conventional photo-cross-linking strategies can be used to identify transient interactions, but these approaches often suffer from high background due to the cross-linked bait proteins. To solve the problem, we have developed membrane-permeable releasable photo-cross-linkers that allow for prey-bait separation after protein complex isolation and can be installed in proteins of interest (POIs) as unnatural amino acids...
October 2017: Nature Protocols
https://www.readbyqxmd.com/read/28933279/zinc-transporter-protein-tzn-1-may-also-play-a-role-in-conidiation-pathway-of-neurospora-crassa-an-insight-using-proteogenomic-approach
#13
Divya Rupa Venkata Pandala, Jogeswar Gadi, Kiranmayi Patnala
Zinc transporter (tzn-1) of Neurospora crassa plays a crucial role in conidiation pathway, as its removal results in aconidiation which was reported in our earlier studies. Present study depicts the role of tzn-1 in conidiation process, by comparing knockout (KO) mutants zinc transporter KO (Δtzn-1) and aconidiating gene KO (Δacon-3) with wild oak ridge (OR) 74 'A' strain by 'Proteo-genomic' approach. Firstly, nine commonly overexpressed protein spots from the two sets (Δtzn-1 to wild and Δacon-3 to wild) were analyzed by combining 2-Dimensional gel electrophoresis (2DE) with Matrix Associated Laser Desorption/Ionization mass spectrometry -Peptide Mass Fingerprint (MALDI-PMF)...
September 19, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28932887/microchip-capillary-gel-electrophoresis-combined-with-lectin-affinity-enrichment-employing-magnetic-beads-for-glycoprotein-analysis
#14
Nicole Y Engel, Victor U Weiss, Christian Wenz, Susanne Glück, Andreas Rüfer, Martin Kratzmeier, Martina Marchetti-Deschmann, Günter Allmaier
Due to the constant search for reliable methods to investigate glycoproteins in complex biological samples, an alternative approach combining affinity enrichment with rapid and sensitive analysis on-a-chip is presented. Glycoproteins were specifically captured by lectin-coated magnetic beads, eluted by competitive sugars, and investigated with microchip capillary gel electrophoresis (MCGE), i.e., CGE-on-a-chip. We compared our results to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) data, which turned out to be in very good agreement...
September 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28932837/enhanced-anodic-electrochemiluminescence-of-cdte-quantum-dots-based-on-electrocatalytic-oxidation-of-a-co-reactant-by-dendrimer-encapsulated-pt-nanoparticles-and-its-application-for-sandwiched-immunoassays
#15
Lu-Lu Ren, Hao Dong, Ting-Ting Han, Yun Chen, Shou-Nian Ding
Herein, we synthesized Pt dendrimer-encapsulated nanoparticles (Pt DENs) using amine-terminated sixth-generation polyamidoamine dendrimers. The enhanced and stable anodic electrochemiluminescence (ECL) of 3-mercaptopropionic acid-capped CdTe quantum dots (QDs) in a tripropylamine solution was achieved owing to Pt DENs. The reason may be that Pt DENs exhibit high catalytic electrochemical oxidation in the presence of tripropylamine and excellent conductive property. Inspired by this, Pt DENs were conjugated with Fe3O4@SiO2 nanoparticles and served as nano-carriers...
September 21, 2017: Analyst
https://www.readbyqxmd.com/read/28932219/improving-acetic-acid-production-by-over-expressing-pqq-adh-in-acetobacter-pasteurianus
#16
Xuefeng Wu, Hongli Yao, Lili Cao, Zhi Zheng, Xiaoju Chen, Min Zhang, Zhaojun Wei, Jieshun Cheng, Shaotong Jiang, Lijun Pan, Xingjiang Li
Pyrroquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) is a key enzyme in the ethanol oxidase respiratory chain of acetic acid bacteria (AAB). To investigate the effect of PQQ-ADH on acetic acid production by Acetobacter pasteurianus JST-S, subunits I (adhA) and II (adhB) of PQQ-ADH were over-expressed, the fermentation parameters and the metabolic flux analysis were compared in the engineered strain and the original one. The acetic acid production was improved by the engineered strain (61.42 g L(-1)) while the residual ethanol content (4...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28932152/human-salivary-protein-extraction-from-rnapro%C3%A2-sal%C3%A2-pure%C3%A2-sal%C3%A2-and-passive-drooling-method
#17
Zohaib Khurshid, Syed Faraz Moin, Rabia Sannam Khan, Muhammad Atif Saleem Agwan, Abdullah Hamed Alwadaani, Muhammad Sohail Zafar
OBJECTIVE: The aim of the current study was to carry out a preliminary validation of devices for standardized collection of whole mouth fluid (WMF) in comparison to the passive drooling method for protein analysis in healthy subjects. MATERIALS AND METHODS: A carefully designed sample collection/pretreatment protocol is crucial to the success of any saliva proteomics project. In this study, WMF was collected from healthy volunteers (n = 10, ages: 18-26 years). Individuals with any oral disease were excluded from the study group...
July 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28930631/direct-evidence-of-intrinsic-blue-fluorescence-from-oligomeric-interfaces-of-human-serum-albumin
#18
Arpan Bhattacharya, Soumitra Bhowmik, Amit K Singh, Prashant Kodgire, Apurba K Das, Tushar Kanti Mukherjee
The molecular origin behind the concentration-dependent intrinsic blue fluorescence of human serum albumin (HSA) is not known yet. This unusual blue fluorescence is believed to be a characteristic feature of amyloid-like fibrils of protein/peptide and originates due to the delocalization of peptide bond electrons through the extended hydrogen bond networks of cross--sheet structure. Herein, by combining the results of spectroscopy, size exclusion chromatography, native gel electrophoresis and confocal microscopy, we have shown that the intrinsic blue fluorescence of HSA exclusively originates from oligomeric interfaces devoid of any amyloid-like fibrillar structure...
September 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28930152/proteomic-analysis-of-the-chlorophyta-dunaliella-new-strain-al-1-revealed-global-changes-of-metabolism-during-high-carotenoid-production
#19
Faten Ben Amor, Fatma Elleuch, Hajer Ben Hlima, Matthieu Garnier, Bruno Saint-Jean, Mohamed Barkallah, Chantal Pichon, Slim Abdelkafi, Imen Fendri
The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions...
September 20, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28929967/-results-of-a-multicenter-study-investigating-plasmid-mediated-colistin-resistance-genes-mcr-1-and-mcr-2-in-clinical-enterobacteriaceae-%C3%A4-solates-from-turkey
#20
Ayşe Nur Sarı, Serap Süzük, Onur Karatuna, Dilara Öğünç, Ayşe Esra Karakoç, Zeynep Çizmeci, Hikmet Eda Alışkan, Füsun Cömert, Mustafa Zahir Bakıcı, Nezahat Akpolat, Fatma Feriha Çilli, Yasemin Zer, Aysel Karataş, Bahar Akgün Karapınar, Gülçin Bayramoğlu, Melda Özdamar, Fatma Kalem, Nuran Delialioğlu, Elif Aktaş, Nisel Yılmaz, Şaban Gürcan, Zeynep Gülay
Colistin is a polymyxin antibiotic which is considered as one of the last line agents against infections due to multidrug resistant or carbapenem resistant gram-negative pathogens. Colistin resistance is associated with chromosomal alterations which can usually cause mutations in genes coding specific two component regulator systems. The first plasmid-mediated colistin resistance gene, mcr-1 was described in Escherichia coli and Klebsiella pneumoniae isolates in December 2015 and followed by another plasmid-mediated colistin resistance gene mcr-2 in 2016...
July 2017: Mikrobiyoloji Bülteni
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