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https://www.readbyqxmd.com/read/28544846/genomic-dna-interactions-mechanize-peptidotoxin-mediated-anti-cancer-nanotherapy
#1
Santosh K Misra, Aaron S Schwartz-Duval, Dipanjan Pan
Host defense peptides (HDPs) are a class of evolutionarily conserved substances of the innate immune response that has been identified as major players in the defense system in many living organisms. Some of the HDPs are also referred as peptidotoxins which offer immense potential for anti-cancer therapy. However, their therapeutic potential is yet to be fully translated mainly due to their off-target toxicity. Here we show that their nano-enabled delivery may become beneficial in controlling their delivery in intra-cellular space...
May 25, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28544236/clinical-and-laboratory-phenotype-variability-in-type-2m-von-willebrand-disease
#2
Ashley L Doruelo, Sandra L Haberichter, Pamela A Christopherson, Lisa N Boggio, Sweta Gupta, Steven R Lentz, Amy D Shapiro, Robert R Montgomery, Veronica H Flood
BACKGROUND: Von Willebrand factor (VWF) is a multimeric coagulation factor that tethers platelets to injured subendothelium. Type 2M von Willebrand Disease (VWD) is characterized by a qualitative defect in VWF with preserved multimer distribution. OBJECTIVES: Through the Zimmerman Program for the Molecular and Clinical Biology for VWD, five VWF sequence variations were studied in subjects diagnosed with type 2M VWD. METHODS: Bleeding phenotype was assessed using the ISTH bleeding assessment tool...
May 20, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28542601/the-epidemiology-of-staphylococcus-aureus-carriage-in-patients-attending-inner-city-sexually-transmitted-infections-and-community-clinics-in-calgary-canada
#3
Alejandra Ugarte Torres, Angel Chu, Ron Read, Judy MacDonald, Daniel Gregson, Thomas Louie, Johanna Delongchamp, Linda Ward, Joann McClure, Kunyan Zhang, John Conly
BACKGROUND: Although the nares represent the most common carriage site for traditional hospital-associated strains of Staphylococcus aureus (SA), the predominant site of carriage of SA in the community is less certain. METHODS: We conducted a cross-sectional study in 285 patients attending sexually transmitted diseases and inner-city clinics to evaluate the prevalence, body site colonisation and risk factors associated with carriage of methicillin susceptible SA (MSSA)...
2017: PloS One
https://www.readbyqxmd.com/read/28542566/proteomic-analysis-in-the-dufour-s-gland-of-africanized-apis-mellifera-workers-hymenoptera-apidae
#4
Aparecida das Dores Teixeira, Patricia D Games, Benjamin B Katz, John M Tomich, José C Zanuncio, José Eduardo Serrão
The colony of eusocial bee Apis mellifera has a reproductive queen and sterile workers performing tasks such as brood care and foraging. Chemical communication plays a crucial role in the maintenance of sociability in bees with many compounds released by the exocrine glands. The Dufour's gland is a non-paired gland associated with the sting apparatus with important functions in the communication between members of the colony, releasing volatile chemicals that influence workers roles and tasks. However, the protein content in this gland is not well studied...
2017: PloS One
https://www.readbyqxmd.com/read/28542247/genetic-polymorphism-of-merozoite-surface-protein-2-msp-2-in-plasmodium-falciparum-isolates-from-pawe-district-north-west-ethiopia
#5
Hussein Mohammed, Moges Kassa, Ashenafi Assefa, Mekonnen Tadesse, Amha Kebede
BACKGROUND: In malaria endemic regions, Plasmodium falciparum infection is characterized by extensive genetic diversity. Describing this diversity provides important information about the local malaria situation. This study was conducted to evaluate the extent of genetic diversity of P. falciparum in Pawe district, North West Ethiopia, using the highly polymorphic merozoite surface protein 2 gene. METHODS: Atotal of 92 isolates from patients with uncomplicated P...
2017: PloS One
https://www.readbyqxmd.com/read/28540712/harnessing-the-power-of-pcr-molecular-fingerprinting-methods-and-next-generation-sequencing-for-understanding-structure-and-function-in-microbial-communities
#6
Sujal Phadke, Andreia Filipa Salvador, Joana Isabel Alves, Orianna Bretschger, Maria Madalena Alves, Maria Alcina Pereira
Polymerase chain reaction (PCR) is central to methods in molecular ecology. Here, we describe PCR-dependent approaches useful for investigating microbial diversity and its function in various natural, human-associated, and built environment ecosystems. Protocols routinely used for DNA extraction, purification, cloning, and sequencing are included along with various resources for the statistical analysis following gel electrophoresis-based methods (DGGE) and sequencing. We also provide insights into eukaryotic microbiome analysis, sample preservation techniques, PCR troubleshooting, DNA quantification methods, and commonly used ordination techniques...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28540349/dataset-of-protein-species-from-human-liver
#7
Stanislav Naryzhny, Maria Maynskova, Victor Zgoda, Alexander Archakov
This article contains data related to the research article entitled "Zipf׳s law in proteomics" (Naryzhny et al., 2017) [1]. The protein composition in the human liver or hepatocarcinoma (HepG2) cells extracts was estimated using a filter-aided sample preparation (FASP) protocol. The protein species/proteoform composition in the human liver was determined by two-dimensional electrophoresis (2-DE) followed by Electrospray Ionization Liquid Chromatography-Tandem Mass Spectrometry (ESI LC-MS/MS). In the case of two-dimensional electrophoresis (2-DE), the gel was stained with Coomassie Brilliant Blue R350, and image analysis was performed with ImageMaster 2D Platinum software (GE Healthcare)...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28539717/caspase-mediated-apoptotic-effects-of-ebenus-boissieri-barbey-extracts-on-human-cervical-cancer-cell-line-hela
#8
Ece Simsek, Nilufer Imir, Esra Arslan Aydemir, Ramazan Suleyman Gokturk, Erdem Yesilada, Kayahan Fiskin
BACKGROUND: Ebenus boissieri Barbey is an Antalya, Turkey-endemic plant belonging to Fabaceae family. The aerial parts and the roots of E. boissieri Barbey were used in this study. OBJECTIVE: In the present study, we have examined the apoptotic effects of hydroalcoholic extracts of E. boissieri Barbey in human cervical cancer cell line HeLa. MATERIALS AND METHODS: To determine the cytotoxic effect, cells were treated with various concentrations of extracts for 24, 48, and 72 h incubation periods...
April 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28539344/investigation-of-kingella-kingae-invasive-infections-outbreaks-in-day-care-facilities-assessment-of-a-rapid-genotyping-tool-targeting-the-dna-uptake-sequence
#9
Philippe Bidet, Violaine Tran Quang, Pablo Yagusky, André Birgy, Stéphane Bonacorsi, Romain Basmaci
Objectives Outbreaks of Kingella kingae invasive infections have recently been reported in day-care centers. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) revealed that although the invasive strains had a widespread dissemination in the day-care population, less virulent strains were also found to be circulating in the facility. However, these typing tools are costly, time-consuming and labor-intensive, and provide delayed results. A study was conducted to assess the performance of a rapid and cost-effective genotyping tool targeting the DNA uptake sequence (DUS) in the investigation of outbreaks of K...
May 24, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28539041/outer-membrane-vesicles-derived-from-salmonella-enteritidis-protect-against-the-virulent-wild-type-strain-infection-in-a-mouse-model
#10
Qiong Liu, Jie Yi, Kang Liang, Xiangmin Zhang, Qing Liu
Food-borne contamination and salmonellosis caused by Salmonella Enteritidis (S. Enteritidis) are a significant threat to human health and poultry enterprises. Outer membrane vesicles (OMVs), which are naturally secreted by gram-negative bacteria, could be a good vaccine option because they have many biologically active substances, including lipopolysaccharides (LPS), outer membrane proteins (OMPs), and phospholipids, as well as periplasmic components. In the present study, we purified OMVs derived from S. Enteritidis and analyzed their characteristics through silver staining and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...
May 25, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28536716/on-chip-hydrodynamic-chromatography-of-dna-through-centimeters-long-glass-nanocapillaries
#11
Lian Duan, Levent Yobas
This study demonstrates hydrodynamic chromatography of DNA fragments in a microchip. The microchip contains a highly regular array of nanofluidic channels (nanocapillaries) that are essential for resolving DNA in this chromatography mode. The nanocapillaries are self-enclosed robust structures built inside a doped glass layer on silicon using low-resolution photolithography and standard semiconductor processing techniques. Additionally, the unique nanocapillaries feature a cylindrical inner radius of 600 nm maintained over a length scale of 5 cm...
May 24, 2017: Analyst
https://www.readbyqxmd.com/read/28535932/charting-novel-allergens-from-date-palm-pollen-phoenix-sylvestris-using-homology-driven-proteomics
#12
Bodhisattwa Saha, Naren Pandey, Swati Gupta Bhattacharya
Pollen grains from Phoenix sylvestris (date palm), a commonly cultivated tree in India has been found to cause severe allergic diseases in an increasing percentage of hypersensitive individuals. To unearth its allergenic components, pollen protein were profiled by two-dimensional gel electrophoresis followed by immunoblotting with date palm pollen sensitive patient sera. Allergens were identified by MALDI-TOF/TOF employing a layered proteomic approach combining conventional database dependent search and manual de novo sequencing followed by homology-based search as Phoenix sylvestris is unsequenced...
May 20, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28535790/clonal-replacement-of-epidemic-kpc-producing-klebsiella-pneumoniae-in-a-hospital-in-china
#13
Yuying Liang, Xiuyun Yin, Lijun Zeng, Shuiping Chen
BACKGROUND: Klebsiella pneumoniae is a frequent nosocomial pathogen causing difficult-to-treat infections worldwide. The prevalence of Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae (KPC-KP) is increasing in China. The aim of this study was to investigate the molecular epidemiology of KPC-KP in a nosocomial outbreak. METHODS: Fifty-four KPC-KP isolates were consecutively collected between November 2013 and August 2014 during a KPC-KP outbreak in a tertiary care hospital in Beijing, China...
May 23, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28534993/analysis-of-the-cdr3-length-repertoire-and-the-diversity-of-t-cell-receptor-%C3%AE-and-%C3%AE-chains-in-swine-cd4-and-cd8-t-lymphocytes
#14
Chun-Yan Wang, Yong-Xiang Fang, Guo-Hua Chen, Huai-Jie Jia, Shuang Zeng, Xiao-Bing He, Yuan Feng, Shou-Jie Li, Qi-Wang Jin, Wen-Yu Cheng, Zhi-Zhong Jing
The T cell receptor (TCR) is a complex heterodimer that recognizes fragments of antigens as peptides and binds to major histocompatibility complex molecules. The TCR α and β chains possess three hypervariable regions termed complementarity determining regions (CDR1, 2 and 3). CDR3 is responsible for recognizing processed antigen peptides. Immunoscope spectratyping is a simple technique for analyzing CDR3 polymorphisms and sequence length diversity, in order to investigate T cell function and the pattern of TCR utilization...
May 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28534953/proteomic-analysis-of-differentially-expressed-proteins-in-kidneys-of-brain-dead-rabbits
#15
Ling Li, Ning Li, Chongxiang He, Wei Huang, Xiaoli Fan, Zibiao Zhong, Yanfeng Wang, Qifa Ye
A large number of previous clinical studies have reported a delayed graft function for brain dead donors, when compared with living relatives or cadaveric organ transplantations. However, there is no accurate method for the quality evaluation of kidneys from brain‑dead donors. In the present study, two‑dimensional gel electrophoresis and MALDI‑TOF MS‑based comparative proteomic analysis were conducted to profile the differentially‑expressed proteins between brain death and the control group renal tissues...
May 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28533374/unique-clade-of-alphaproteobacterial-endosymbionts-induces-complete-cytoplasmic-incompatibility-in-the-coconut-beetle
#16
Shun-Ichiro Takano, Midori Tuda, Keiji Takasu, Naruto Furuya, Yuya Imamura, Sangwan Kim, Kosuke Tashiro, Kazuhiro Iiyama, Matias Tavares, Acacio Cardoso Amaral
Maternally inherited bacterial endosymbionts in arthropods manipulate host reproduction to increase the fitness of infected females. Cytoplasmic incompatibility (CI) is one such manipulation, in which uninfected females produce few or no offspring when they mate with infected males. To date, two bacterial endosymbionts, Wolbachia and Cardinium, have been reported as CI inducers. Only Wolbachia induces complete CI, which causes 100% offspring mortality in incompatible crosses. Here we report a third CI inducer that belongs to a unique clade of Alphaproteobacteria detected within the coconut beetle, Brontispa longissima This beetle comprises two cryptic species, the Asian clade and the Pacific clade, which show incompatibility in hybrid crosses...
May 22, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28532454/proteomic-profiling-of-peripheral-blood-neutrophils-identifies-two-inflammatory-phenotypes-in-stable-copd-patients
#17
Adèle Lo Tam Loi, Susan Hoonhorst, Corneli van Aalst, Jeroen Langereis, Vera Kamp, Simone Sluis-Eising, Nick Ten Hacken, Jan-Willem Lammers, Leo Koenderman
BACKGROUND: COPD is a heterogeneous chronic inflammatory disease of the airways and it is well accepted that the GOLD classification does not fully represent the complex clinical manifestations of COPD and this classification therefore is not well suited for phenotyping of individual patients with COPD. Besides the chronic inflammation in the lung compartment, there is also a systemic inflammation present in COPD patients. This systemic inflammation is associated with elevated levels of cytokines in the peripheral blood, but the precise composition is unknown...
May 22, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28532090/in-situ-synthesis-of-bilayered-gradient-poly-vinyl-alcohol-hydroxyapatite-composite-hydrogel-by-directional-freezing-thawing-and-electrophoresis-method
#18
Cui Su, Yunlan Su, Zhiyong Li, Muhammad Abdul Haq, Yong Zhou, Dujin Wang
Bilayered poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) composite hydrogels with anisotropic and gradient mechanical properties were prepared by the combination of directional freezing-thawing (DFT) and electrophoresis method. Firstly, PVA hydrogels with aligned channel structure were prepared by the DFT method. Then, HA nanoparticles were in situ synthesized within the PVA hydrogels via electrophoresis. By controlling the time of the electrophoresis process, a bilayered gradient hydrogel containing HA particles in only half of the gel region was obtained...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531233/in-silico-development-and-characterization-of-tri-nucleotide-simple-sequence-repeat-markers-in-hazelnut-corylus-avellana-l
#19
Gehendra Bhattarai, Shawn A Mehlenbacher
Plant genomes are now sequenced rapidly and inexpensively. In silico approaches allow efficient development of simple sequence repeat markers, also known as microsatellite markers, from these sequences. A search of the genome sequence of 'Jefferson' hazelnut (Corylus avellana L.) identified 8,708 tri-nucleotide simple sequence repeats with at least five repeat units, and stepwise removal of the less promising sequences led to the development of 150 polymorphic markers. Fragments in the 'Jefferson' sequence containing tri-nucleotide repeats were used as references and aligned with genomic sequences from seven other cultivars...
2017: PloS One
https://www.readbyqxmd.com/read/28530597/dna-agarose-gel-electrophoresis-for-antioxidant-analysis-development-of-a-quantitative-approach-for-phenolic-extracts
#20
Sara Silva, Eduardo M Costa, Sandra Vicente, Mariana Veiga, Conceição Calhau, Rui M Morais, Manuela E Pintado
Most of the fast in vitro assays proposed to determine the antioxidant capacity of a compound/extract lack either biological context or employ complex protocols. Therefore, the present work proposes the improvement of an agarose gel DNA electrophoresis in order to allow for a quantitative estimation of the antioxidant capacity of pure phenolic compounds as well as of a phenolic rich extract, while also considering their possible pro-oxidant effects. The result obtained demonstrated that the proposed method allowed for the evaluation of the protection of DNA oxidation [in the presence of hydrogen peroxide (H2O2) and an H2O2/iron (III) chloride (FeCl3) systems] as well as for the observation of pro-oxidant activities, with the measurements registering interclass correlation coefficients above 0...
October 15, 2017: Food Chemistry
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