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https://www.readbyqxmd.com/read/29142827/overexpression-of-the-primary-sigma-factor-gene-siga-improved-carotenoid-production-by-corynebacterium-glutamicum-application-to-production-of-%C3%AE-carotene-and-the-non-native-linear-c50-carotenoid-bisanhydrobacterioruberin
#1
Hironori Taniguchi, Nadja A Henke, Sabine A E Heider, Volker F Wendisch
Corynebacterium glutamicum shows yellow pigmentation due to biosynthesis of the C50 carotenoid decaprenoxanthin and its glycosides. This bacterium has been engineered for production of various non-native cyclic C40 and C50 carotenoids such as β-carotene, astaxanthin or sarcinaxanthin. In this study, the effect of modulating gene expression more broadly by overexpression of sigma factor genes on carotenoid production by C. glutamicum was characterized. Overexpression of the primary sigma factor gene sigA improved lycopene production by recombinant C...
June 2017: Metabolic Engineering Communications
https://www.readbyqxmd.com/read/29140702/influence-of-the-carotenoid-composition-on-the-conformational-dynamics-of-photosynthetic-light-harvesting-complexes
#2
Marijonas Tutkus, Jevgenij Chmeliov, Danielis Rutkauskas, Alexander V Ruban, Leonas Valkunas
Non-photochemical quenching (NPQ) is the major self-regulatory mechanism of green plant, performed on a molecular level to protect them from an over-excitation during the direct sunlight. It is believed that NPQ becomes available due to conformational dynamics of the light-harvesting photosynthetic complexes and involves a direct participation of carotenoids. In this work, we perform a single-molecule microscopy on major light-harvesting complexes (LHCII) from different Arabidopsis thaliana mutants exhibiting various carotenoid composition...
November 15, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29138423/the-photocycle-of-orange-carotenoid-protein-conceals-distinct-intermediates-and-asynchronous-changes-in-the-carotenoid-and-protein-components
#3
E G Maksimov, N N Sluchanko, Y B Slonimskiy, E A Slutskaya, A V Stepanov, A M Argentova-Stevens, E A Shirshin, G V Tsoraev, K E Klementiev, O V Slatinskaya, E P Lukashev, T Friedrich, V Z Paschenko, A B Rubin
The 35-kDa Orange Carotenoid Protein (OCP) is responsible for photoprotection in cyanobacteria. It acts as a light intensity sensor and efficient quencher of phycobilisome excitation. Photoactivation triggers large-scale conformational rearrangements to convert OCP from the orange OCP(O) state to the red active signaling state, OCP(R), as demonstrated by various structural methods. Such rearrangements imply a complete, yet reversible separation of structural domains and translocation of the carotenoid. Recently, dynamic crystallography of OCP(O) suggested the existence of photocycle intermediates with small-scale rearrangements that may trigger further transitions...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29137491/characterization-of-biochemical-traits-of-dog-rose-rosa-canina-l-ecotypes-in-the-central-part-of-iran
#4
Milad Javanmard, Hossein Ali Asadi-Gharneh, Pejman Nikneshan
Dog rose (Rosa canina L.) is a wild native species in Iran, with a significant genetic diversity. This plant serves as a rich source of vitamin C, anthocyanins, phenolic contents and carotenoids. Rose hips have been used in several food products, as well as perfumery and cosmetics industries. In this research, we investigate biochemical characteristics of five dog rose ecotypes (Kopehjamshid, Zarneh, Miyankish, Aghcheh and Sadeghiyeh), that were collected from the central part of Iran (Isfahan province). Amounts of vitamin C, total carotenoids, total phenolic contents, total anthocyanins, macro and micro minerals were measured...
November 14, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29137113/integrated-evaluation-of-the-potential-health-benefits-of-einkorn-based-breads
#5
Fabiana Antognoni, Roberto Mandrioli, Alessandra Bordoni, Mattia Di Nunzio, Blanca Viadel, Elisa Gallego, María Paz Villalba, Lidia Tomás-Cobos, Danielle Laure Taneyo Saa, Andrea Gianotti
Nowadays the high nutritional value of whole grains is recognized, and there is an increasing interest in the ancient varieties for producing wholegrain food products with enhanced nutritional characteristics. Among ancient crops, einkorn could represent a valid alternative. In this work, einkorn flours were analyzed for their content in carotenoids and in free and bound phenolic acids, and compared to wheat flours. The most promising flours were used to produce conventional and sourdough fermented breads. Breads were in vitro digested, and characterized before and after digestion...
November 11, 2017: Nutrients
https://www.readbyqxmd.com/read/29136934/differential-responses-of-the-green-microalga-chlorella-zofingiensis-to-the-starvation-of-various-nutrients-for-oil-and-astaxanthin-production
#6
Xuemei Mao, Tao Wu, Dongzhe Sun, Zhao Zhang, Feng Chen
Chlorella zofingiensis has been proposed as a potential producer of lipids and the high-value carotenoid astaxanthin. In this study, the responses of photoautotrophic C. zofingiensis with respect to growth, lipid profiles and astaxanthin accumulation were investigated upon the starvation of N (NS), P (PS) and S (SS). NS and SS stimulated triacylglycerol (TAG) accumulation, which reached 27% and 21% of dry weight (DW), respectively. Stresses also stimulated astaxanthin accumulation greatly, reaching 3.9 mg/g DW by NS...
October 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29134501/pharmacokinetic-properties-of-saffron-and-its-active-components
#7
Azar Hosseini, Bibi Marjan Razavi, Hossein Hosseinzadeh
Saffron as a medicinal plant has many therapeutic effects. Phytochemical studies have reported that saffron is composed of at least four active ingredients which include crocin, crocetin, picrocrocin and safranal. The carotenoids of saffron are sensitive to oxygen, light, heat and enzymatic oxidization. However, regulation of these factors is required for saffron quality. Some pharmacologic effects of saffron and its active compounds include cardioprotective, neuroprotective, memory enhancer, antidepressant and anxiolytic...
November 13, 2017: European Journal of Drug Metabolism and Pharmacokinetics
https://www.readbyqxmd.com/read/29132754/the-engineering-potential-of-rhodosporidium-toruloides-as-a-workhorse-for-biotechnological-applications
#8
REVIEW
Young-Kyoung Park, Jean-Marc Nicaud, Rodrigo Ledesma-Amaro
Moving our society towards a bioeconomy requires efficient and sustainable microbial production of chemicals and fuels. Rhodotorula (Rhodosporidium) toruloides is a yeast that naturally synthesizes substantial amounts of specialty chemicals and has been recently engineered to (i) enhance its natural production of lipids and carotenoids, and (ii) produce novel industrially relevant compounds. The use of R. toruloides by companies and research groups has exponentially increased in recent years as a result of recent improvements in genetic engineering techniques and the availability of multiomics information on its genome and metabolism...
November 10, 2017: Trends in Biotechnology
https://www.readbyqxmd.com/read/29131551/%C3%AE-cryptoxanthin-inhibits-angiogenesis-in-human-umbilical-vein-endothelial-cells-huvec-through-retinoic-acid-receptor-rar
#9
José Manuel Quesada-Gómez, Raquel Santiago-Mora, Mario Durán-Prado, Gabriel Dorado, Gema Pereira-Caro, José Manuel Moreno-Rojas, Antonio Casado-Díaz
SCOPE: β-Cryptoxanthin is an abundant carotenoid in fruits and vegetables that can be quantified in human blood-serum. Yet, contrary to other carotenoids, its effects on endothelial cells and angiogenesis remain unknown. METHODS AND RESULTS: Human umbilical-vein endothelial cells (HUVEC) were treated with 0.01, 0.1 or 1 μM of β-cryptoxanthin. Antioxidant activity was determined by its free radical scavenging and oxygen-radical absorbance capacity (ABTS and ORAC assays)...
November 13, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29128781/photosynthesis-impairments-and-excitation-energy-dissipation-on-wheat-plants-supplied-with-silicon-and-infected-with-pyricularia-oryzae
#10
Carlos Eduardo Aucique-Pérez, Paulo Eduardo de Menezes Silva, Wiler Ribas Moreira, Fábio Murilo DaMatta, Fabrício Ávila Rodrigues
Considering the effect of silicon (Si) in reducing the blast symptoms on wheat in a scenario where the losses in the photosynthetic capacity of the infected plants is lowered, this study investigated the ability of using the incident light, the chloroplastidic pigments (chlorophylls and carotenoids) alterations and the possible role of carotenoids on the process of light dissipation on wheat plants non-supplied (-Si) or supplied (+Si) with Si and inoculated or not with Pyricularia oryzae. For + Si plants, blast severity was reduced compared to -Si plants...
October 26, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29126411/thermal-conditions-during-early-life-influence-seasonal-maternal-strategies-in-the-three-spined-stickleback
#11
Sin-Yeon Kim, Neil B Metcalfe, Alberto da Silva, Alberto Velando
BACKGROUND: Conditions experienced by a female during early life may affect her reproductive strategies and maternal investment later in life. This effect of early environmental conditions is a potentially important mechanism by which animals can compensate for the negative impacts of climate change. In this study, we experimentally tested whether three-spined sticklebacks (Gasterosteus aculeatus) change their maternal strategy according to environmental temperatures experienced earlier in life...
November 10, 2017: BMC Ecology
https://www.readbyqxmd.com/read/29125620/metabolite-profiling-of-peppers-of-various-colors-reveals-relationships-between-tocopherol-carotenoid-and-phytosterol-content
#12
Tae Jin Kim, Jaehyuk Choi, Kil Won Kim, Soon Kil Ahn, Sun-Hwa Ha, Yongsoo Choi, Nam Il Park, Jae Kwang Kim
Peppers are widely consumed in Korea; the varietal development of peppers with increased content of beneficial plant metabolites is, therefore, of considerable interest. This requires a comprehensive understanding of the metabolic profile of pepper plants and the factors affecting this profile. To this end, we determined the content of various metabolites, such as hydrophilic and lipophilic compounds, phenolic acids, carotenoids, and capsaicinoids in peppers of various colors (green, red, pale green, and violet peppers) and in a high-pungency (green) pepper...
November 10, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/29124966/marine-natural-pigments-as-potential-sources-for-therapeutic-applications
#13
Panchanathan Manivasagan, Subramaniyan Bharathiraja, Madhappan Santha Moorthy, Sudip Mondal, Hansu Seo, Kang Dae Lee, Junghwan Oh
In recent years, marine natural pigments have emerged as a powerful alternative in the various fields of food, cosmetic, and pharmaceutical industries because of their excellent biocompatibility, bioavailability, safety, and stability. Marine organisms are recognized as a rich source of natural pigments such as chlorophylls, carotenoids, and phycobiliproteins. Numerous studies have shown that marine natural pigments have considerable medicinal potential and promising applications in human health. In this review, we summarize the marine natural pigments as potential sources for therapeutic applications, including: antioxidant, anticancer, antiangiogenic, anti-obesity, anti-inflammatory activities, drug delivery, photothermal therapy (PTT), photodynamic therapy (PDT), photoacoustic imaging (PAI), and wound healing...
November 10, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/29124852/occurrence-of-chlorophyll-allomers-during-virus-induced-mortality-and-population-decline-in-the-ubiquitous-picoeukaryote-ostreococcus-tauri
#14
Deborah J Steele, Susan A Kimmance, Daniel J Franklin, Ruth L Airs
During viral infection and growth limitation of the picoeukaryote Ostreococcus tauri, we examined the relationship between membrane permeability, oxidative stress and chlorophyll allomers (oxidation products). Chlorophyll allomers were measured in batch-cultures of O. tauri in parallel with maximum quantum efficiency of photosystem II photochemistry (Fv /Fm ), carotenoids, and reactive oxygen species and membrane permeability using fluorescent probes (CM-H2 DCFDA and SYTOX-Green). Viral infection led to mass cell lysis of the O...
November 10, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/29124704/biofilms-microbial-strategies-for-surviving-uv-exposure
#15
Carla C C R de Carvalho
Biofilm communities are an ingenious form of protection of microbial cells which have been evolving for billion of years. In general, ultraviolet (UV) radiation presents poor penetration in the matrix of biofilms and only the first few top layers of microbial cells are exposed to its deleterious effects. For further protection against UV radiation, exposed cells can produce specialized compounds such as mycosporine-like amino acids and carotenoid pigments. In this chapter, the adaptation mechanisms presented by biofilms against UV radiation are presented, as well as the application of UV light to monitor and destroy biofilms in man made surfaces...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29124652/red-shift-in-the-spectrum-of-a-chlorophyll-species-is-essential-for-the-drought-induced-dissipation-of-excess-light-energy-in-a-poikilohydric-moss-bryum-argenteum
#16
Yutaka Shibata, Ahmed Mohamed, Koichiro Taniyama, Kentaro Kanatani, Makiko Kosugi, Hiroshi Fukumura
Some mosses are extremely tolerant of drought stress. Their high drought tolerance relies on their ability to effectively dissipate absorbed light energy to heat under dry conditions. The energy dissipation mechanism in a drought-tolerant moss, Bryum argenteum, has been investigated using low-temperature picosecond time-resolved fluorescence spectroscopy. The results are compared between moss thalli samples harvested in Antarctica and in Japan. Both samples show almost the same quenching properties, suggesting an identical drought tolerance mechanism for the same species with two completely different habitats...
November 9, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/29120792/influence-of-different-cultivation-systems-on-bioactivity-of-asparagus
#17
Yang Gyu Ku, Dong Hyeon Kang, Choung Keun Lee, Si Young Lee, Chan Seok Ryu, Dong Eok Kim, Martin Polovka, Jacek Namieśnik, Shela Gorinstein
Evaluation of functional and bioactive compounds of Asparagus officinalis L. growing in conventional and organic greenhouse and conventional open - field farming was the aim of this research. Polyphenols in cladodes grown conventionally were higher than organic. Flavonoids and carotenoids were the highest in cladodes in open field. Organic spears were richer in total phenolics (+6.9% and +19.1%) and flavonoids (+4.7% and 16.8%) and showed higher in vitro averages of antioxidant activities by three radical scavenging assays (+12...
April 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29120782/targeted-secondary-metabolic-and-physico-chemical-traits-analysis-to-assess-genetic-variability-within-a-germplasm-collection-of-long-storage-tomatoes
#18
Laura Siracusa, Cristina Patanè, Valeria Rizzo, Salvatore Luciano Cosentino, Giuseppe Ruberto
"Long storage" tomato is a crop traditionally cultivated in the Mediterranean area under no water supply, that recently has attracted the interest of breeders for its high tolerance to drought and as potential genetic source in breeding programs for water stress resistance. A collection of 28 genotypes of "long storage" tomato (Solanum lycopersicum L.) was studied for carotenoid and polyphenol profile and content, vitamin C, and other physico-chemical traits of fruits. Tomato carotenoids and polyphenols were identified and quantified using high-performance liquid chromatography coupled with diode array detection and electrospray-mass spectrometry (HPLC/DAD/ESI-MS); nineteen different phenolic compounds and six different carotenoids, for a total of 25 markers, have been detected, quantified and used to discriminate among the different landraces to find out which could be the best candidate for a medium-to-large scale cultivation...
April 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29120759/untargeted-metabolomics-reveals-specific-withanolides-and-fatty-acyl-glycoside-as-tentative-metabolites-to-differentiate-organic-and-conventional-physalis-peruviana-fruits
#19
Sandra M Llano, Ana M Muñoz-Jiménez, Claudio Jiménez-Cartagena, Julián Londoño-Londoño, Sonia Medina
The agronomic production systems may affect the levels of food metabolites. Metabolomics approaches have been applied as useful tool for the characterization of fruit metabolome. In this study, metabolomics techniques were used to assess the differences in phytochemical composition between goldenberry samples produced by organic and conventional systems. To verify that the organic samples were free of pesticides, individual pesticides were analyzed. Principal component analysis showed a clear separation of goldenberry samples from two different farming systems...
April 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29120377/concentrations-of-carotenoids-and-tocopherols-in-breast-milk-from-urban-chinese-mothers-and-their-associations-with-maternal-characteristics-a-cross-sectional-study
#20
Yong Xue, Esther Campos-Giménez, Karine Meisser Redeuil, Antoine Lévèques, Lucas Actis-Goretta, Gerard Vinyes-Pares, Yumei Zhang, Peiyu Wang, Sagar K Thakkar
Milk composition remains the best estimate of infant requirements. The aims of this study were to quantify carotenoids and tocopherols in human milk from healthy Chinese mothers, and to explore their associations with lactation stage, region, socio-economic and obstetric characteristics, and dietary intake. Human milk was obtained from 509 healthy mothers, and concentrations of carotenoids and tocopherols were analyzed by Ultra High Performance Liquid Chromatography. The mothers' socio-economic and obstetric characteristics and dietary intake through a single 24-h dietary recall were evaluated...
November 9, 2017: Nutrients
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