Ali Reza Ahmadi, Guang Song, Tianshun Gao, Jing Ma, Xiaomei Han, Ming-Wen Hu, Andrew M Cameron, Russell N Wesson, Benjamin Philosophe, Shane Ottmann, Elizabeth King, Ahmet Gurakar, Le Qi, Brandon Peiffer, James Burdick, Robert Anders, Zhanxiang Zhou, Hongkun Lu, Dechun Feng, Chien-Sheng Chen, Jiang Qian, Bin Gao, Heng Zhu, Zhaoli Sun
The pathogenesis of antibodies in severe alcoholic hepatitis (SAH) remains unknown. We analyzed immunoglobulins (Ig) in explanted livers from SAH patients (n=45) undergoing liver transplantation and tissues from corresponding healthy donors (HD, n=10) and found massive deposition of IgG and IgA isotype antibodies associated with complement fragment C3d and C4d staining in ballooned hepatocytes in SAH livers. Ig extracted from SAH livers, but not patient serum exhibited hepatocyte killing efficacy. Employing human and Escherichia coli K12 proteome arrays, we profiled the antibodies extracted from explanted SAH, livers with other diseases, and HD livers...
December 6, 2023: ELife